Risk Assessmen Guidelines

Faculty of Engineering

Activity or Experiment-Based Risk Assessment Form Department: Division of Bioengineering Name of Experiment/Activity: UV crosslinking of polyacrylamide gel Location: E3A-07-02 Name of Person in-charge: Zhu Qingdi Name of PI: Dr. Dieter Trau Last Review Date: 250308 Next Review Date: 250310

1. Hazard Identification 2. Risk Evaluation & Control Existing Risk Additional / Possible No. Task Hazards Control S L R New Risk S L R Action By Deadline Consequences (if any) Control 1. Place Sample Electricity Electrical Distance, keep 3 1 3 into the Shock/Fire wire untangled UVCrosslinker InvolatileToxic Toxic effects Gloves 3 2 6 Use containers 3 1 3 Zhu 260308 Samples: to contain the Qingdi Acrylamide etc. sample when exposing Glass Slides and Breakage causing Gloves 1 2 2 Glass Masks cuts 2 Turn on the UV exposure UV leakage or UV Auto-cut-off of the 2 2 4 Log on book, 2 1 2 Zhu 260308 crosslinker and overheating UV when door prevent long Qingdi exposure opened time, high E exposure Conducted by: (Name, designation) Approved by: (Name, designation) Signature: Signature: Date: Date: Risk Assessment Matrix

Likelihood Severity Remote (1) Occasional (2) Frequent (3) Minor (1) 1 2 3 Moderate (2) 2 4 6 Major (3) 3 6 9

Severity Categories Level Human (Impact to Biological Impact Environmental Property Damage (S$) Physical Being) Damage

(1) Minor No Injury or light injury May not cause human disease, if does, the disease Reversible Up to $5,000 requiring only first aid is unlikely to spread to the community and there is treatment (MC < 4 days usually effective prophylaxis or treatment available; MC) (2) Moderate Any injury/ill health Can cause severe human disease, not ordinarily Reversible but takes $5,001 to $50,000 leading to ≥ 4 days MC or spread by casual contact from one individual to years ≥ 1 day hospitalisation or another; it may spread to the community, but there leads to temporary is usually effective prophylaxis or treatment disability available

(3) Major Fatality, permanent Can cause lethal human disease, may be readily Irreversible More than $50,000 Disability or life transmitted from one individual to another, or from threatening disease animal to human or vice-versa directly or indirectly, or casual contact, it may spread to the community; usually no effective prophylaxis or treatment available

Likelihood Categories Level Events Frequency (1) Remote Undesired event which may occur but unlikely, once in 5 years (2) Possible Undesired event which is probable, once in a year (3) Frequent Undesired event which probably occur in most circumstances, once or more in a month

Risk Level and Acceptability Criteria Acceptability Risk Score Risk Level Recommended Actions of Risk No additional risk control measures required. To continue to monitor to ensure risk do not escalate to higher level. <3 Low Risk Acceptable 3 – 4 Medium Risk Moderately Acceptable to carry out the work activity; however, task need to be reviewed to bring risk level to As Low As Reasonably Acceptable Practicable.

Interim control measures such as administrative controls can be implemented. Supervisory oversight required. Job must not be carried out until risk level is brought to at least medium risk level.

Risk controls should not be overly dependant on personal protective equipment. Controls measures should focus on Elimination, substitution and engineering controls. >4 High Risk Not Acceptable Immediate Management intervention required to ensure risk being brought down to at least medium level before work can be commenced.