Name______Date______Period______Genetic Technology WebQuest

LinkLink #1 #1: http://www.ornl.gov/sci/techresources/Human_Geno me/home.shtml 1. When was the Human Genome Project completed?

2. Fill in the blanks that address the goals of the human genome project:  identify all the approximately ______genes in human DNA,  determine the sequences of the ______chemical base pairs that make up human DNA,  store this information in ______,  improve tools for ______,  transfer related technologies to the ______,  Address the ______(ELSI) that may arise from the project.  Link #2: http://www.vtaide.com/png/cloning.htm  Central Dogma of Biochemistry 3.Link Finish #2 this sentence: The central theme underlying all of biochemistry is that…

4. Which molecule serves as an intermediate between a gene and a protein?

Link #3 Link #3: Go http://learn.genetics.utah.edu/content/cloning/clickandclone/ (If this link doesn’t work- you can Google “Mimi the Mouse” and it is the first link). 5. What are you asked to remove from the egg?

6. What are you asked to remove from the somatic cell?

7. Where are you asked to put the nucleus from the somatic cell?

8. How is Mimi involved in the process of cloning Mimi?

9. What color is the baby mouse (pup)?

10. When and where was this procedure used in science? What did scientists learn from their research?

LinkLink #4 #4 Go back to http://www.medicalnewstoday.com/info/stem_cell/ Name______Date______Period______

11. Where are stem cells?

12. What are the two types of stem cells and what are the differences between them?

LinkLink #5 #6: http://www.dnai.org/d/index.html  DNA Applications  Human Application Click on “Profiling”

15. List some places you could take a DNA sample from.

Click on “Family” 16. Describe the first practical test of DNA fingerprinting.

Go through the slides, click on A: Try the comparison.

17. What was the outcome of this case?

 Click on the “Murder” Read the case of the state of Florida vs. Jones and Reesh, Murder at Rodman Dam, 1988

18. Click on Letter A to match the fingerprints yourself. Who raped Perry?

LinkLink #6 #7: http://learn.genetics.utah.edu/content/labs/gel/ Continue to click through the animation to answer the following questions. 19. What does the liquid contain?

20. How can we sort the DNA samples even though we can’t see them?

21. How can we make DNA move through the gel? 22. What kind of charge is on the end with the wells? ______

23. What size strands move most quickly through the gel?

24. What shows up as bands in the gel? Now it’s your turn… Name______Date______Period______

25. What are the 5 steps to making a gel electrophoresis? (Hint: look at the top right of the screen).

26. What is needed to make the gel?

27. What does the buffer do for the gel?

28. What forms the holes, or “wells” in gel?

29. Explain the purpose of putting the “DNA Standard” into one of the wells.

30. What kind of charge does DNA have?

31. What lets us know that the current is running through the gel?

32. What do we add to the DNA in order to see the bands?

33. How do we measure the estimated length of the bands?

33. What is you estimate for the size of the first band? Second band? Third band? Name______Date______Period______

LinkLink #7 #8: //http://www.csa.com/discoveryguides/gmfood/overview.php

34. Describe what “GM” means.

35. What is used to create GMOs?

36. . What were the two most harvested crops in America in 2000?

37. According to data taken in 2000, who was the leading producer of transgenic crops?

38. What are the possible outcomes (positive or negative) for using GMOs?

39. What do scientists predict are future transgenic crops and what is their purpose?

40. What percentage of cross contamination is accepted in GMO?

41. Out of all of the possible benefits to GM crops, 42. Out of all the possible controversies of GM

which three do you think are most important? crops, which three do you think are most

 important?

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Extra Credit:  Take “The Cloning Quiz” on the following link: http://www.vtaide.com/png/cloning.htm Score: ______ Try “Is it cloning or not?” from the following link: http://learn.genetics.utah.edu/content/tech/cloning/cloningornot/  How’d you do? OR  Go to http://www.ornl.gov/sci/techresources/Human_Geno me/home.shtml  Research  Gene Gateway  Genetic Disorder Guide  Genes and Disease. Look up the one of the following genetic disorders and briefly describe it below: (i.e. what causes it, how does it affect the individual, what is the mode of inheritance, can it be cured, etc).  List of Diseases to research: Hemophilia, muscular dystrophy, color blindness, Huntington’s, PKU, Tay-Sachs, Sickle Cell Anemia, and Cystic Fibrosis.