Supplementary Table 1. F Values Obtained from the Analysis of Variance of Data Shown in Table 1

30 60 kDa s ue 25

val 20

nsi 15

ed ed 10

eg Int 0

PR114 PR121 PR122 PR118

s 70 ue 33 kDa

val 60 31 kDa ty ty 50

nsi 40 de

ed ed 30 rat 20

eg 10 Int 0

PR114 PR121 PR122 PR118 Supplementary Figure 1. Densitometry scanning of 60kDa, 33 kDa and 31 kDa (glutelin acidic subunits). Total proteins extracted from the ripened seeds harvestedfrom the indicated cultivars after different dates of transplantationwere separated by SDS-PAGE. AlphaEaseFC version 6.0.0 software wasused for densitometry scanning. s 30 28 kDa

val 26 kDa

ty ty 20

ed ed 10

s PR114 PR121 PR122 PR118

eg Int

PR114 PR121 PR122 PR118 Supplementary Figure 2. Densitometry scanning of globulins (28 kDa and 26 kDa), glutelin basic subunits (24 kDa and 22 kDa).

ue 40 20 kDa

35val 16 kDa

ty ty 30 15 kDa

nsi 25 de 20

ed ed 15

10rat eg Int 5 0

PR114 PR121 PR122 PR118 Supplementary Figure 3. Densitometry scanning of prolamines of 20 kDa, 16 kDa and 15 kDa. V m

Time (min)

Supplementary Figure 4. Chromatogram of amino acids standard.

Supplementary Table 1. F values obtained from the analysis of variance of data shown in Table 1

F-values

Source L* PR a*PR b*PR Whiteness HRY Chalky AC F BV S λmax S grains Cultivar 57690** 12582** 106834** 14241** 245541** 406** 106404** 2686** 501.56**

TD 18676** 2115** 4489** 11554** 86300** 154** 10941** 2065** 170.91** Cultivar 10983** 1143** 50276** 3972** 7298** 8.38** 13277** 2455** 52.73** × TD

TD= transplantation date; *= p ≤ 0.05; **= p ≤ 0.005; NS=non-significant; HRY= head rice yield; F= flour; AC= amylose content; BV= blue value; S= starch. Supplementary Table 2. F-values obtained from the analysis of variance of data shown in Table 2

Source F-values AAs Cultivar TD Cultivar x TD Asp 1668** 387** 1460** Glut 409** 2874** 696** Aspr 151** 273** 45** Ser 1688** 7425** 2873** Glu 3727** 2290** 681** His 1040** 1792** 386** Thr 960** 7852* 4277** Arg 138** 5630** 1914** GABA 11** 45.78** 10.54** Tyr 91.50** 294** 91** Cys 1106** 2504** 151** Val 1626** 5789** 464** Met 484** 1762** 277** Try 68.25** 665** 413** Phe 909** 2309** 53.58** I-Leu 507** 1552* 363** Lys 99.66** 244** 3.70** Pro 3067** 13859** 7232** TD= transplantation date; *= p ≤ 0.05; **= p ≤ 0.005; NS=nonsignificant; Tyr= tyrosine; Cys= cystine; Val= valine; Met= methionine; Try= Tryptophan, Phe= phenyl- alanine; I-leu= isoleucine; Lys= lysine; Pro= proline; Asp= aspartic acid; Glut= glutamic acid; Aspr= asparagine; Glu= glutamine; His= histidine; Thr= threonine; Arg=arginine; GABA=λ-amino butyric acid. Supplementary Table 3. F values obtained from the analysis of variance of data shown in Table 3

Source Fvalues

GS GS GS GS To Tp Tc PT PV BD SB FV (0-5μm) (5-10 μm) (10-20 μm) (20-60 μm) (˚C) (˚C) (˚C)

Cultivar 420689** 14814** 65824** 34882** 79358** 122044** 57.61** 110311** 227092** 478818** 530523** 39071**

TD 203249** 25879** 43718** 8430** 3353** 14240** 11.13** 603046** 2723969** 1102569* 216112** 99323** Cultivar 183304** 6543** 9084** 3363** 5372** 10681** 9.26** 235163** 93172** 38311** 648560** 13853** × TD

TD= transplantation date; *= p ≤ 0.05; **= p ≤ 0.005; NS=non significant; To= onset temperature; Tp =peak temperature; Tc=conclusion

temperature; ΔHgel= enthalpy of gelatinization, GS= Granule size; PT= pasting temperature; PV= peak viscosity; FV= final viscosity; BD= breakdown viscosity; SB= setback viscosity.