University of Louisville s6

Department: Date SOP was written: Principal Investigator: Building: Laboratory: Primary Emergency Contact (Name and Number): Secondary Emergency Contact (Name and Number):

Hydrogen Gas Synonyms: Dihydrogen; o-Hydrogen; p-Hydrogen; Molecular hydrogen; H2; UN 1049

Clemson University Standard Operating Procedure

1. Working Principle: Enter what general purpose this chemical will be having in this laboratory. Examples include DNA purification procedures, preservation of biological material, and as a solvent in organic synthesis.

2. Physical and Chemical Properties

CAS #: 1333-74-0 Molecular Formula: H2 Physical State (Form): Compressed Gas Potential Hazards: Extremely flammable gas, Contains gas under pressure, Simple Asphyxiant Incompatibilities: Oxidizing Agents, Oxygen Gas May form explosive mixtures in Air.

3. Health Effects

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MERGEFORMATINET

Signal word: Danger

Precautionary Statement(s): P210 Keep away from heat/sparks/open flames/hot surfaces. No smoking. P377 Leaking gas fire: Do not extinguish, unless leak can be stopped safely. P381 Eliminate all ignition sources if safe to do so. P410 + P403 Protect from sunlight. Store in a well-ventilated place.

Inhalation: May displace oxygen and cause rapid suffocation Vapors may cause dizziness or asphyxiation without warning

Skin exposure: Contact with rapidly expanding gas may cause burns or frostbite

Eye exposure: Contact with rapidly expanding gas may cause burns or frostbite

Additional Information: Extremely flammable gas Contains gas under pressure; may explode if heated Burns with invisible flame May form explosive mixtures in Air May displace Oxygen and cause rapid suffocation

4. Regulatory Review:

Hydrogen Gas contains no substances with occupational exposure limit value.

Last Modified: July 2017 Hydrogen Gas is a Simple Asphyxiant, therefore all work must be performed in a well ventilated area with an emergency air supply procedure in place.

5. Controlling Exposure:

All procedures involving Hydrogen Gas must be performed in a properly functioning chemical fume hood. The fume hood is labeled-

Designated Area Danger Hydrogen Gas Extremely flammable gas, Contains gas under pressure, Simple Asphyxiant AUTHORIZED PERSONNEL ONLY

Storage: Store away from incompatible materials. Such as oxidizers including oxygen gas.

6. Personal Protective Equipment (PPE)

Respiratory Protection: Respiratory protection should not be necessary for lab personnel if the appropriate safe work practices and use proper ventilation are followed.

Hand Protection: Nitrile gloves are recommended. Gloves must be inspected prior to use. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices.

Always check with your glove manufacturer to make sure that the gloves are compatible with the hazardous material in the laboratory. Check these websites by glove manufacturers to ensure that the glove material will offer adequate protection.

Chemical Resistant Glove Directory - http://www.chemrest.com/

Microflex - http://www.microflex.com/products/~/media/files/literature/domestic%20reference %20materials/dom_reference_chemical%20resistance.ashx

Ansell - http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf

Eye Protection: ANSI approved safety glasses or chemical splash goggles.

Skin and Body Protection: Flame- resistant Lab coats should be worn and buttoned. Sleeves should be sufficient to prevent skin exposure while wearing gloves. Lab personnel should also wear full length pants (or equivalent) and close-toed shoes.

7. Spill Control:

Last Modified: July 2017 In case of critical cylinder failure, immediately evacuate area and ensure others are aware of the release. If there is an imminent threat of a fire, pull the nearest fire alarm station to evacuate the building and dial 911.

8. Waste Disposal:

Return empty gas cylinders to manufacturer if leased. If the gas cylinder is not leased, contact Research Safety for further direction. For questions regarding hazardous waste disposal contact the Hazardous Materials Manager, 633-6357.

9. Emergency Procedures:

In Case of Accidental Release: Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Remove all sources of ignition. Evacuate personnel to safe areas. Beware of vapors accumulating to form explosive concentrations. Vapors can accumulate in low areas.

In Case of Critical Cylinder Failure: In case of catastrophic cylinder failure, immediately evacuate area and ensure others are aware of the release. If there is an imminent threat of a fire, pull the nearest fire alarm station to evacuate the building and dial 911. Contact CUFD at 911 or 656-2222.

Inhalation: If inhaled, remove to fresh air. If breathing becomes difficult, seek medical attention. Contact CUFD at 911 or 656-2222.

Skin/Clothing Contact: Remove contaminated clothing and rinse body thoroughly in emergency shower for at least 15 minutes. Then seek medical attention. CUFD at 911 or 656-2222.

Eye Contact: Rinse eyeball and inner surface of eyelid in the emergency eyewash station for at least 15 minutes. Then seek medical attention. CUFD at 911 or 656-2222.

Ingested: Do NOT induce vomiting. If swallowed, wash out mouth with water. Then seek medical attention immediately. Contact CUFD at 911 or 656-2222.

10.Gas Cylinder Handling and Storage:

Precautions for safe handling:  Avoid inhalation of vapors or mist.  Store and use away from heat, sparks, open flame or any other ignition source – No Smoking  Take measures to prevent the buildup of electrostatic charge; Use explosion-proof electrical equipment.  Empty containers retain product residue and can be hazardous.  Do not puncture or incinerate container.  Use equipment rated for cylinder pressure.  Close valves after each use and when empty.  Protect cylinders from physical damage; do not drag, roll, slide, or drop.

Conditions for Safe Storage:

Last Modified: July 2017  Store in a segregated and approved area.  Store away from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials.  Eliminate all ignition sources.  Keep containers tightly closed and sealed until read for use.  Cylinders should be stored upright, with valve protection cap in place, and firmly secured to prevent falling or being knocked over.

See section 5 of the Lab Safety Manual for additional Compress Gas Cylinder Safety information.

11.Lab-Specific Procedures: Enter the specific instructions the laboratory personnel will have to use when working with the hazardous chemical. This may include solvent preparation and usage, etc.

12.Documentation of Training (signature of all users is required)  Prior to conducting any work with Hydrogen Gas, designated personnel must provide training to his/her laboratory personnel specific to the hazards involved in working with this substance, work area decontamination, and emergency procedures.

 The Principal Investigator must ensure that his/her laboratory personnel have completed all required safety training.

I have read and understand the content of this SOP: Name Signature Date

Last Modified: July 2017 Last Modified: July 2017