Characterization and Expression Profile Analysis of a Sucrose Synthase Gene from Common
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1 Characterization and expression profile analysis of a sucrose synthase gene 2 from common bean (Phaseolus vulgaris L.) during seed development
3Ghassen Abid (1, 2)*, Yordan Muhovski2, Jean-Marie Jacquemin2, Dominique Mingeot2, 4Khaled Sassi3, André Toussaint1, Jean-Pierre Baudoin1
5(1) University of Liège-Gembloux Agro-Bio Tech. Unit of Tropical Crop Husbandry and Horticulture, 6Gembloux Agricultural University, Passage des Déportés 2, B-5030 Gembloux, Belgium.
7(2) Department of Life Sciences, Unit of Breeding and Biodiversity, Walloon Agricultural Research 8Centre, Chaussée de Charleroi, 234, B-5030 Gembloux, Belgium.
9(3) University of Carthage, National Agronomy Institute of Tunisia (INAT), Department of Agronomy 10and Plant Biotechnology, Laboratory of Agronomy, Avenue Charles Nicolle, 43, 1082 -Tunis- 11Mahrajène, Tunisia.
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13*Author for correspondence: Ghassen Abid
14 Unit of Tropical Crop Husbandry and Horticulture
15 Passage des Déportés, 2
16 5030 Gembloux
17 Belgium
18 Tel : 003281/622110
19 Fax : 003281/614544
20 E-mail : [email protected]
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24 25Supplementary data
26Supplement Fig. 1 Multiple alignment of the deduced amino acid sequences of Pv_BAT93 27Sus and Sus sequences from other species. The GenBank database accession numbers of the 28displayed Sus genes are: Sus from Ps, Pisum sativum (CAA09910); Pv_BAT93, Phaseolus 29vulgaris (cultivar BAT93) (ACP17902); Gh, Gossypium hirsutum (AAD28641); Ta, Triticum 30aestivum (CAA04543); Pv, Phaseolus vulgaris (AAN76498); and St, Solanum tuberosum 31(CAD61188). The alignment was produced by the alignment program of BoSS, which uses 32the ClustalW algorithm. The identical amino acids are indicated by a black background. 33Similar amino acids are highlighted by a gray background. The conserved blocks and
34invariant residues are shown above the alignments. Boxed Ser11 residue indicates the putative 35phosphorylation site conserved in almost all Sus described. Bold underline indicates the 36putative transmembrane domains
37Supplement Fig. 2 A neighbor-joining tree derived from the phylogenetic comparisons of 38Sus genes collected in an international database (GenBank). The numbers along the branches 39are bootstrap values (1000 replicates). The scale bar indicates the genetic distance for 0.02 40amino acid substitution/site. The GenBank accession numbers for the sequences are: 41At_SUS1, Arabidopsis thaliana SUS1 (At5g20830); At_SUS2, Arabidopsis thaliana SUS2 42(At5g49190); At_SUS4, Arabidopsis thaliana SUS4 (At3g43190); At_SUS3, Arabidopsis 43thaliana SUS3 (NM_116461); Citu_SUS1, Citrus unshiu (AB022092); Gh, Gossypium 44hirsutum (U73588); Gm, Glycine max (AF030231); Hv, Hordeum vulgare SUS1 (X65871); 45Le_SUS1, Lycopersicum esculentum SUS1 (L19762); Le_SUS2, Lycopersicum esculentum 46SUS2 (AJ011319), Ms, Medicago sativa (AF049487); Os_SUS1, Oryza sativa SUS1 47(X64770); Os_SUS2, Oryza sativa SUS2 (X59046), Os_SUS3, Oryza sativa SUS3 (L03366); 48Ps, Pisum sativum (AJ01208); Pt_SuSY1, Populus trichocarpa SuSY1 (XM_002324100); 49Pt_SuSY2, Populus trichocarpa SuSY2 (XM_002326273); Pv, Phaseolus vulgaris 50(AF315375); Pv_BAT93, Phaseolus vulgaris (cultivar BAT93) (FJ853595); St_SUS3, 51Solanum tuberosum SUS3 (U24088); St_SUS4, Solanum tuberosum SUS4 (U24087); 52Ta_SUS1, Triticum aestivum SUS1 (AJ001117); Ta_SUS2, Triticum aestivum SUS2 53(AJ000153); Vf, Vicia faba (X 69773); Vr, Vigna radiata (D10266); Zm_SH1, Zea mays 54SH1 (X02400); Zm_SUS1, Zea mays SUS1 (L22296); and Zm_SUS3, Zea mays SUS3 55(AY059416) 56Supplement Fig. 3 Southern-blot analysis of common bean genomic DNA. A 387 bp Sus 57cDNA fragment was [α-32P] labeled to probe a blot of common bean genomic DNA at high 58stringency. DNA samples (10 µg) were digested with the following restriction endonucleases: 59EcoRI (E), HindIII (H), and XbaI (Xb). HindIII-digested bacteriophage λ DNA was used as a 60size marker. Length markers are given on the left in kilobases
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