Human Anatomy and Physiology II Lecture (BIOL 252-171)

Microbiology Lab (BIOL 2230) – Fall 2013 PREP SHEETS

WK Topics Orgs Media 8 Gram Stain & Microscope Practice Per Table (Gram Per Student: Stain Controls): 1 NAP (streak) 10/16 Students will Gram stain one slide using control organisms plus their assigned organism. NAS of E. coli 1 CIT slant NAS of S. aureus 1 PAD slant Streaking Practice 1 UREA slant Students will streak ONE plate from their assigned Each student will 1 BE slant organism. be given ONE of 1 SIM tube the following Biochemical Testing II: Enzymes organisms. All For Class Cultures :  Catalase (5-4) biochemical 19 NAS 5 TGB  S. aureus testing will be performed on each  E. faecalis organism.  Oxidase (5-5) Instructor Cultures:  E. coli NAS for None  P. aeruginosa Biochem:  Citrate Utilization (5-7)  P.  E. coli aeruginosa-2  E. aerogenes slants  Phenylalanine Deamination (5-9)  A.  E. coli faecalis-2 slants  P. vulgaris  E. coli  Bile Esculin Hydrolysis (5-10)  E.  E. coli aerogenes  P. vulgaris  E.  K. pneumoniae cloacae-2  Urea Hydrolysis (5-12) slants  E. coli  K.  P. vulgaris pneumoniae  K. pneumoniae  K. oxytoca  Sulfur Indole Motility (SIM) (5-17)  P.  E. coli mirabilis  S. flexneri  P.  S. typhimurium vulgaris  K. pneumoniae  S. marcescens  S. typhimurium  S. flexneri  M. luteus  S. aureus  S. epidermidis  S. saprophyticu s

Thioglycollate Broths for Biochem:  S. pyogenes  S. agalactiae  S. peumonia e  S. bovis  E. faecalis