Online Resources for “A sea lamprey sex pheromone mixture increases trap catch relative to a single synthesized component in specific environments” by Johnson et al.

Online Resource Fig 1. Ocqueoc River experimental site where sea lampreys were targeted on spawning areas with pheromone-baited traps. Red lines indicate PIT antennas, blue lines indicate net leads directing lamprey into the trap, and blue rectangles indicate mini-hoop traps.

1 ONLINE RESOURCE TABLE 1. MINERS AND ROCK RIVER ODORANT APPLICATION SUMMARY Average difference in Average concentration 3kPZS pumped per per Average 3kPZS Concentration River Treatment Year Trialsa pumped (g)b day (mg)c day (mg)d (M)e 6.09E-15 Miners 3kPZS 2012 41 0.193 4.8 (0.91) ( 8.97E-14) 5.05E-13 ( 1.01E-13) SMW 41 0.195 4.9 ( 1.15) NAf 5.11E-13 ( 1.25E-13)

8.09E-15 Miners 3kPZS 2013 49 0.392 8.2 ( 3.94) (3.59E-14) 4.86E-13 ( 6.56E-14) SMW 49 0.383 8 ( 3.58) NAf 4.79E-13 ( 4.43E-14)

7.07E-15 Rock 3kPZS 2013 47 0.401 8.5 ( 2.92) ( 3.57E-14) 5.10E-13 ( 6.1E-14) SMW 47 0.403 8.6 ( 2.63) NAf 5.17E-13 ( 5.2E-14)

a Total successful trials of the experiment excluding failures.

b Total amount of 3kPZS pumped to each river each year.

c Average amount of 3kPZS pumped each day standard deviation.

d The difference between the two solutions standard deviation.

e Average amount of 3kPZS concentration pumped over the experiment standard deviation.

f NA, not applicable

ONLINE RESOURCE TABLE 2. CANDIDATE MODELS EXPLAINING THE VARIABILITY IN WHICH

TRAP SEA LAMPREYS WERE CAPTURED IN AT THE MINERS AND ROCK RIVERS DURING 2012 AND

2013

Model - Fixed Effectsab Model - Random Effects logLik AIC Delta i Wi Rankc 0.0 SMW -442.7 (df=2) 889.4 25.50 0 8 0.0 SMW + Sex -439.5 (df=5) 889.01 25.11 0 7 0.0 SMW+Trapdate -434.1 (df=3) 874.17 10.27 1 3 0.0 SMW+Trapdate+Sex -431.2 (df=6) 874.33 10.43 1 4 0.0 SMW+Trapdate+SMW:Trapdate -433.7 (df=4) 875 11.10 1 5 0.0 SMW Stream -440.9 (df=3) 887.8 23.90 0 6 SMW+Trapdate Stream -428.0 (df=4) 863.9 0.00 0.6 1

2 9 0.2 SMW+Trapdate Stream+Year -428.0 (df=5) 865.9 2.00 9 2

a SMW was applied to one trap and the other trap was 3kPZS-baited.

b Trapdate is the day of the trapping season where day one was the first day a sea lamprey was captured in that stream and year.

c Ranks were determined by weighted AIC (Wi) which were calculated from differences (Deltai) in Akaike’s Information Criterion (AIC) values.

ONLINE RESOURCE TABLE 3. OCQUEOC RIVER ODORANT APPLICATION SUMMARY Average Average difference 3kPZS pumped per pumped per Average 3kPZS River Treatment Year Trialsa pumped (g)b trial (mg)c trial (mg)d Concentration (M)e

Ocqueoc 3kPZS 2012 10 0.144 14.4 ( 0.78) 0.5 ( 0.8) 5.82E-13 ( 1.5E-13)

SMW 10 0.149 14.9 ( 0.20) NAf 5.97E-13 ( 1.3E-13)

Ocqueoc 3kPZS 2013 23 0.63 27.4 (2.31) 0.6 (3.2) 9.62E-13 ( 3.56E-13)

SMW 23 0.618 26.9 ( 2.44) NAf 9.42E-13 ( 3.49E-13)

a Total successful trials of the experiment excluding failures.

b Total amount of 3kPZS pumped to each river each year.

c Average amount of 3kPZS pumped each day standard deviation.

d The difference between the two solutions standard deviation.

e Average amount of 3kPZS concentration pumped over the experiment standard deviation.

f NA, not applicable

ONLINE RESOURCE TABLE 4. CANDIDATE MODELS EXPLAINING THE VARIABILITY IN WHICH TRAP SEA LAMPREYS WERE CAPTURED IN AT THE OCQUEOC RIVER DURING 2012 Model - Fixed Effectsab logLik AIC Delta i Wi Rankc

3 SMW -164.3 (df=2) 332.59 11.52 0.01 7 SMW + Sex -157.6 (df=5) 325.11 4.04 0.14 2 SMW + Sex + SMW:Sex -155.8 (df=8) 327.63 6.56 0.05 3 SMW + Trapdate -162.3 (df=3) 330.57 9.50 0.01 4 SMW + Trapdate + SMW:Trapdate -161.8 (df=4) 331.65 10.58 0.01 6 SMW + Sex + Trapdate -154.5 (df=6) 321.07 0.00 0.78 1 SMW + Sex + Trapdate + SMW:Sex:Trapdate -154.5 (df=6) 330.76 9.69 0.01 5

a SMW was applied to one trap and the other trap was 3kPZS-baited.

b Trapdate is the day of the trapping season where day one was the first day a sea lamprey was captured in that stream and year.

c Ranks were determined by weighted AIC (Wi) which were calculated from differences (Deltai) in Akaike’s Information Criterion (AIC) values.

ONLINE RESOURCE TABLE 5. OCQUEOC RIVER TOTAL SPAWNING NESTS OBSERVATIONS OVER THE COURSE OF THE EXPERIMENTAL TRIALS # males Total Total Active observe # females Average males per Average females per Year nests nestsa d observed active nestb active nestb 2012 240 95 118 82 1.31 ( 0.59) 0.72 ( 0.69) 2013 148 67 70 106 1.04 ( 0.55) 1.58 ( 1.36)

a Sea lampreys that were present at spawning nests during observations.

b Total number of males/females divided by total active nests standard deviation.

ONLINE RESOURCE TABLE 6. CANDIDATE MODELS EXPLAINING THE VARIABILITY IN WHICH TRAP SEA LAMPREYS WERE CAPTURED IN AT THE OCQUEOC RIVER DURING 2013

Model - Fixed Effectsab logLik AIC Delta i Wi Rankc SMW -58.2 (df=2) 120.43 120.43 0.58 1 SMW + Sex -56.7 (df=5) 123.39 123.39 0.22 3 SMW + Trapdate -58.1 (df=3) 122.3 122.30 0.35 2

a SMW was applied to one trap and the other trap was 3kPZS-baited.

b Trapdate is the day of the trapping season where day one was the first day a sea lamprey was captured in that stream and year.

4 c Ranks were determined by weighted AIC (Wi) which were calculated from differences (Deltai) in Akaike’s Information Criterion (AIC) values.

5