Direct Electrochemistry and Electrocatalysis of Microperoxidase-11 Immobilized on Porous
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A study on the direct electrochemistry and electrocatalysis of microperoxidase-11 immobilized
on a porous network-like gold film: Sensing of hydrogen peroxide
Qian-Li Zhang,a,b Ai-Jun Wang,a Zi-Yan-Meng,a Ya-Hui Lu,c Hong-Jun Lin,a Jiu-Ju Fenga* aCollege of Chemistry and Life Science, College of Geography and Environmental Science, Zhejiang
Normal University, Jinhua 321004, China bSchool of Chemistry and Biological Engineering, Suzhou University of Science and Technology,
Suzhou, 215009, China cSchool of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007,
China
*Corresponding author: [email protected], Tel./Fax: +86 579 82282269
B 350 ) A (
t 0 n
e r r u C -350
-700 0.0 0.5 1.0 1.5 Potential(V)
Fig. S1. The CVs of the porous network-like Au film modified electrode in 0.5 M H2SO4 solution.
Scan rate: 100 mV·s–1.
1
A h -4 A
-2 /
a t n e
r 0 r u
C 2
4
-0.8 -0.6 -0.4 -0.2 0.0 Potential / V
1.6 B ) A
μ 0.8 (
t n
e r
r 0.0 u C -0.8
28 56 84 112 -1 Scan rate ( mV s )
Fig. S2 (A): Typical CVs on the MP-11/cysteamine/Cu@Au/GCE at different scan rates (curve a-h):
10, 20, 30, 40, 50, 60, 70, and 100 mV·s–1 in 20 mM phosphate solution (pH 7.0). (B): The oxidation and reduction currents vs. scan rates.
20 h 15
A 10
/
t 5 n a e
r r 0 u C -5
-10
-15 -0.8 -0.6 -0.4 -0.2 0.0 0.2 0.4 Potential / V
Fig. S3 Typical CVs on the MP-11/cysteamine/Cu/GCE electrode with different scan rates (curve a-
2 h): 10, 20, 30, 40, 50, 60, 70, and 100 mV·s–1 in 20 mM phosphate solution (pH 7.0).
3
6 A
3 ) A μ
( t
n 0
e r r u C -3
-6 3 4 5 6 7 8 9 pH
-0.18 B Oxidation potential Reduction potential -0.24 ) V (
l a
i -0.30 t n
e t o
P -0.36
-0.42
3.0 4.5 6.0 7.5 9.0 pH
Fig. S4 (A) The oxidation and reduction currents vs. pH. (B) The oxidation and reduction potentials vs. pH.
4
6
3 ) A
μ 0 (
t
n a e r r -3 u C
-6 f -0.8 -0.6 -0.4 -0.2 0.0 Potential (V)
Fig. S5 Typical CVs towards the catalytic reduction of H2O2 on the MP-
11/cysteamine/Cu@Au/GCE in 20 mM N2 saturated phosphate solution with different
concentrations of H2O2 (curve a-f): 0, 0.010, 0.019, 0.039, 0.058, and 0.078 mM. Scan rate: 100
mV·s–1.
45 without H2O2 with 0.039 mM H O 30 2 2 )
A 15 (
t
n e
r 0 r u C -15
-30 -0.8 -0.6 -0.4 -0.2 0.0 0.2 0.4 potential (v)
Fig. S6 Typical CVs of the catalysis of H2O2 on the MP-11/cysteamine/Cu/GCE in 20 mM N2 saturated phosphate solution (pH 7.0) in the absence and presence of 0.039 mM H2O2. Scan rate: 100 mV·s-1.
5 Table S1 Examples of heme proteins based mediator-free H2O2 biosensors.
Materials Stability Linear ranges Detection Refs.
limit
MP-11/Au film 94% (2 weeks) 10 µM ~ 14 mM 0.4 μM Our work
−4 Fe3O4@ SiO2@hemin 85% (4 weeks) 1.0 µM ~ 0.16 mM 2.2×10 [1]
HRP/AuNPs 83 % (12 weeks) 8.0 µM ~ 3.0 mM 2.0 μM [2]
Cyt c/Ag nanocorals 95% (2 weeks) 1.8 μM ~ 23 mM 1.8 μM [3]
Hb/WO3 94% (2 weeks) 3.7µM ~ 0.56 mM 1.5 μM [4]
HRP/GNPs-TNTs 90% (3 days) 5.0 µM ~1.0 mM 2.1 μM [5]
Mb/ZrO2/chitosan 90% (2 weeks) 10.0 µM ~ 1.5 mM 4.0 μM [6]
{Hb/CMK-3}n no change (30 days) 1.2 ~ 57 µM 0.6 μM [7]
Mb/CDA-[bmim]BF4 95% (2 weeks) 5.0~100 μM 2.0 μM [8]
HRP/PTBA PBCs > 90% (1 month) 1 ~ 300 μM 1 μM [9]
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Horseradish peroxidase-catalyzed synthesis of poly(thiophene-3-boronic acid) biocomposites for mono-/bi-enzyme immobilization and amperometric biosensing. Biosens Bioelectron: ASAP.
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