Florida State University

Young Scholars Program Biochemistry Schedule - Summer 2013 Lectures and Labs: 2:00-5:00 pm, 2061 King Life Sciences Building

Monday Tues Wednesday Thurs Friday June 17 18 19 20 21 Chromosome Lab Fruit fly Lab Start blood cultures Observe flies Start crosses 1 & 2

24 25 26 27 28 Chromosome Lab Chromosome Lab Chromosome Lab Process cells Group A: Prepare slides Group B: Prepare slides Group A: 2:00 Stain with Geimsa Stain with Geimsa Group B: 3:30 Observe slides Observe slides Fruit fly Lab Fruit fly Lab Group A: Remove parents Group B: Remove parents July 1 2 3 4 5 Chromosome Lab Plasmid Lab Plasmid Lab Prepare slides for Isolate plasmid DNA Restriction enzyme trypsin staining digestion Fruit fly Lab Observe progeny cross 2 Start cross 3 8 9 10 11 12 Plasmid Lab ABO Lab ABO Lab Gel of digests Group B: Cheek DNA Qiagen column ABO Lab prep and PCR Plasmid Lab Group A: Cheek Discussion of results DNA prep and PCR Fruit fly Lab Remove parents 15 16 17 18 19 ABO Lab ABO Lab Fruit fly Lab Gel of PCR Prepare dilutions for Observe progeny of sequencing cross 3 Chromosome Lab Observe trypsin slides? 22 23 24 25 26 - Exam Chromosome Lab ABO Lab Prepare karyotypes? Results of sequencing Blood typing