Melanoma Associated Antigen (Mutated) 1 (36%)

Table S1 Summary of amino acid sequence homology analysis by NCBI Protein Blast Program with 124 tryptic peptides identified from CA215 by MALDI-TOF MS Amino Acid Sequence of Tryptic Peptides Sequence Homology Searched (%) 1. AAAGGLAMLTSMRPTLCSR Immunoglobulin heavy chain variable region (68%)

ICAM-3 (36%)

2. ADQGSETPSCLEVQCWGSR CD55 antigen, decay accelerating factor for complement (Cromer blood group), isoform CRA_j (52%)

Protocadherin alpha-10 isoform 3 precursor (36%)

3. AEALRTLTK Nestin (100%)

Microtubule-based motor (77%)

4. AFGSAAAFCWLCPCRCWK Adhesion molecule with Ig-like domain 1 (27%)

5. AGLLCHLDDACISNPCHK Delta and Notch-like epidermal growth factor-related receptor precursor (72%)

Dynein heavy chain 14, axonemal isoform 1 (83%)

Cadherin, EGF LAG seven-pass G-type receptor 1 (flamingo homolog, Drosophila), isoform CRA_a (66%)

6. ALACSGMKIGR ligatin, isoform CRA_c (54%)

TCR (72%)

MHC class I antigen (90%)

7. ANESGLAMTDISSLASQNK Melanoma associated antigen (mutated) 1 (36%) Tubulin gamma-1 chain (57%)

1 Cathepsin F (63%)

8. ANPQCMGQNHK Prolow-density lipoprotein receptor-related protein 1 precursor (90%)

9. APQLLCYAATNLQSGGPSR Anti-tetanus toxoid immunoglobulin light chain variable region (100%)

10. APTVVLHMTK Ig heavy chain variable region, VH3 family (70%)

Mucin 5AC protein (70%)

NF-IL6-beta protein (80%) 11. AQCVVSLAPR Interleukin-1 receptor accessory protein isoform 3 (50%)

MEGF10 protein, isoform CRA_b (100%)

Obscurin (80%)

12. ATVASGISPGWMGLDCGPESNK Circulating B cell antibody heavy chain variable region (59%)

Immunoglobulin E heavy chain variable region (50%)

Multiple epidermal growth factor-like domains protein 11 precursor (40%)

13. ATVMGSKCHR Mucin-16 (70%)

14. AVMNGLAPGSNGQDKDMDLTK Cellular myosin heavy chain (38%)

Fc receptor-like A (100%)

15. AVPSAEPQAGSPMTLSCQTK Leukocyte immunoglobulin-like receptor subfamily A member 2 soluble isoform 2 (85%) 16. CDVLK WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2, isoform CRA_b (100%) 17. CNGLMTIVFEENSKVTVPHMIK Immunoglobulin lambda chain variable region (59%)

2 18. DGLLYDTLVLINLESCDKK Nuclear receptor ROR-gamma isoform b (47%)

19. DHPYISEIK Fat-like cadherin FATJ protein (77%)

20. EAHFVRTSGK neural cell adhesion molecule 1, isoform CRA_h (70%)

21. EEAGKEEPPPLTPPAR T-box 3 (ulnar mammary syndrome), isoform CRA_d (100%)

22. EGAAGAFLPACK Multiple epidermal growth factor-like domains protein 8 (66%)

Immunoglobulin heavy chain variable region (58%)

23. EGGTQKTSTLPAVLYSCGICK Immunoglobulin heavy chain variable region (28%)

24. EMTDATNEEETEIKEDTK Ryanodine receptor 2 (cardiac), isoform CRA_c (77%)

25. GDGTSQGCAVPVESDPRHPR Pro-epidermal growth factor isoform 3 preproprotein (65%)

Titin (90%)

26. GDRVTITWR Immunoglobulin kappa chain variable region (100%)

Immunoglobulin light chain variable region (77%)

27. GELSPGHFLSGFSAAGASV Ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin), isoform CRA_b (31%)

28. GLCASAPFSCCLEQDVSRK T cell receptor beta variable 19 (36%)

Immunoglobulin mu heavy chain (63%)

29. GLEWVSAVSGSGTTTYYADSVK Immunoglobulin heavy chain variable region (100%)

Immunoglobulin E heavy chain variable region

3 (100%)

Immunoglobulin alpha heavy chain variable region (100%)

Anti-GM2 glanglioside IgM heavy chain variable region (100%)

30. GQPTYQICPRIIPTTESSCR NWD1 protein (30%)

31. GSSGSSGKCETK N.A.

32. GTEGLCPLLCHADGTPLGAGAR Sialic acid-binding Ig-like lectin 6 isoform 6 precursor (63%)

CD33L1 [Homo sapiens] >gb|AAK83655.1| sialic acid binding immunoglobulin-like lectin 6 (63%)

CD33L2 (63%)

Latent TGF-beta binding protein-4 (31%) 33. HESHRAVEFYQVVCVVFVFYYAK Receptor-type tyrosine-protein phosphatase beta isoform a (34%)

Protein tyrosine phosphatase, receptor type, B (34%)

34. HIISHSGNNPYGCEECGK CLL-associated antigen KW-4 splice variant 2 (94%)

35. IGTMVEDHR Titin (100%)

Complement component C8 beta chain preproprotein (55%)

36. KGGHSSPASSDK Mast cell-expressed membrane protein (75%)

37. KGYIPSYLDK Thyroid hormone receptor beta isoform (100%)

Immunoglobulin kappa 1 light chain (70%)

38. KHPSTVQVK T-cell leukemia translocation altered gene, isoform CRA_b (88%)

4 B-cell receptor CD22 isoform 3 precursor (77%)

V-set and immunoglobulin domain-containing protein 10-like (88%)

39. KTDQMVFHLETNSNSESHDK Protein tyrosine phosphatase, non-receptor type 20A (55%)

Breast cancer-associated antigen SGA-72M (60%)

40. LEPGNCKADETASK Protein-coupled receptor 112 (100%)

Cancer antigen 1 (71%)

41. LGLRCVTCNYCSQLCK Deformed epidermal autoregulatory factor 1 variant (93%)

42. LNTPTDSTLWVSLCCPR Titin isoform novex-2 (64%)

CDC42 binding protein kinase alpha (DMPK-like) (52%)

43. LSPLESRPPCPAGLVR Immunoglobulin kappa light chain variable region (100%)

Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1, isoform CRA (68%)

Discoidin domain receptor family, member 1 (56%)

Receptor tyrosine kinase (56%)

44. LSTGVFGGGTK Immunoglobulin lambda light chain VLJ region (100%)

Immunoglobulin V lambda/J lambda light chain (90%)

45. MAYLDQTLAGPSGTR Immunoglobulin kappa chain variable region (53%)

46. MCSCPISSPGHIKGLGQEETPVR Interleukin 23 receptor (30%)

5 Kainate receptor subunit KA2a (65%)

Glutamate receptor subunit (47%)

47. MIDSVMSCQQLTGLK Dynein heavy chain 2, axonemal (93%)

Dynein heavy chain domain 3, isoform CRA_c (86%)

48. MMGSTHIYDMSTVMSR Progestin and adipoQ receptor family member III, isoform CRA_b (50%)

49. MPIMLRSSNCVLTGK Vascular endothelial growth factor D preproprotein (73%)

T cell receptor alpha-chain V-region (V-J-C) precursor (73%)

50. MSLEWLVAWSWSLDGLR CD19 antigen, isoform CRA_c (52%)

51. MSLLHKHQESGCSGCPR Relaxin receptor 2 isoform 1 (35%)

52. MSPSLFLPAMKEGCNAYK Nebulin isoform 2 (94%)

53. MSSNCTSTTAVAVAPLSASK Ig heavy chain (40%)

Mucin-19-like (100%)

54. MSSPGPSQPPAEDPPWPAR Myopodin protein (84%)

55. MSTMRLLTLALLFSCSVAR Fibroblast growth factor receptor 4, isoform CRA_a (47%)

56. MSTRYHQAASDSYLELLK Dynein, axonemal, heavy chain 11 (100%)

57. MVKSYFCYAEQELMK Insulin-like growth factor 2 mRNA binding protein 1,

6 isoform CRA_b (46%)

58. MVMHARSGGNLEVMGLMLGK Receptor-type protein tyrosine phosphatase beta, RPTP beta, PTP zeta [human, brainstem, Peptide, 2307 aa] (45%）

Receptor-type tyrosine-protein phosphatase U isoform 2 precursor (65%)

Interleukin-20 receptor subunit beta precursor (70%)

59. NTDEPMGITLKMLNHCIVAR 55 kDa erythrocyte membrane protein isoform 4 (70%)

60. PATAPSCPSGSR Epidermal growth factor receptor isoform a variant (66%)

Mucin 5 (83%)

61. SAEPRGGVQVTTQQPR Cingulin (56%)

62. SEMVLYIQSEPVSQDAKPTGHNR Cell adhesion kinase beta (30%)

63. SESEEALPLDLDKNIHTPVSRPNR Major histocompatibility complex, class I, E (83%)

64. SGRPEELVSCADCGRSGGSGR Retinoid X receptor, alpha, isoform CRA_a (71%)

65. SISRPSSLRMSR Breast cancer nuclear receptor-binding auxiliary protein (66%)

66. SIVHPSYNSNTLNNDIMLIK Cathepsin 6 preprotein (75%) 67. SLSCESLWPIEAR Latrophilin-3 precursor (92%) 68. SMPTNCVWLDGLSSNVSDQYLTR Vascular endothelial growth factor receptor 1 isoform 4 precursor (73%)

Dynein heavy chain domain 1 isoform 1 (39%)

Cadherin-7 (56%)

Neuronal cell adhesion molecule isoform E precursor

7 (47%)

Immunoglobulin heavy chain (56%)

69. SPAQYQVVLSERLQALVNSLCAGQSP Tumor necrosis factor receptor superfamily member EDAR precursor (46%)

Trabeculin-alpha (100%)

70. SSGTSYPDVLK Immunoglobulin heavy chain variable region (100%)

Immunoglobulin gamma 1 heavy chain variable region (72%)

71. STNLPRNPSMADYEAR Protocadherin-8 isoform 2 precursor (50%)

72. TAAEADMDAAAGPPVSTRQSK Nuclear receptor co-repressor (100%)

73. TAKGSTGMEILLSTLENTK Immunoglobulin A heavy chain variable region (94%)

Immunoglobulin heavy chain variable region VH (78%)

Immunoglobulin gamma 3 heavy chain variable region (94%）

74. TAKPASQEHARCSAIGTGESPK Profilaggrin (86%) 75. TATIIIHANASMSHTILLK Cadherin-related family member 4 precursor (36%)

76. TGDPSPCPTPSHFDGVCDISCCEVK Mucin-2 precursor (96%) 77. TRTSFTAEQLYR Coronin, actin binding protein, 1B, isoform CRA_c (58%)

78. TSGATQTTVGDALFTR MHC class I antigen (56%)

Interleukin-9 (50%)

79. TSTGAVRMQPK Immunoglobulin gamma 2 heavy chain variable region (81%)

80. TVAMAGVAAMTAVTR HLA-B associated transcript 3 (100%)

8 81. TVCVLSERR Protocadherin Fat 4 precursor (66%)

82. TVGVIVSREAMTGR Cadherin-like 23 (71%)

Interleukin-1 receptor associated kinase-2 (57%)

83. TYFCQQSYTTPLTFGGGTK Immunoglobulin kappa light chain variable region (100%)

IgM monoclonal antibody light chain variable region (100%)

84. VCHSNLIESR B-cell lymphoma 6 protein isoform 2 (50%)

Olfactory receptor, family 52, subfamily E, member 8 (100%)

85. VHTGEKPYGCSECEKNLGK Zinc finger protein 614 (100%)

86. VSSHSSQATKDSGVGLK Immunoglobulin epsilon heavy chain variable region (41%)

87. YLAAAFPSACGK Activin A receptor, type IB, isoform CRA_c (50%)

88. APAHHPAAISTAK MHC class I antigen (38%)

89. APWWSSCRWR Leukocyte immunoglobulin-like receptor subfamily B member 5 (50%)

Carcinoembryonic antigen-related cell adhesion molecule 8 precursor (100%)

90. AEDTAVYYCAKJLTIR Immunoglobulin heavy chain variable region (100%)

91. AELGGLLSPR Immunoglobulin heavy chain subgroup VIII V-D-J region (70%)

9 92. AETLVFSTHAVISMR Immunoglobulin heavy chain variable region (66%)

93. DGSISILGSDDATTCHIVVLR Colony stimulating factor 1 receptor (61%)

Interleukin 1 recepter-like 2 (47%)

94. GDRVTITWR Immunoglobulin kappa chain variable region (100%)

95. GGNSGGSSSICYVLLGFIGTSK T cell receptor beta variable 7 (68%)

96. GLECIGYMYSSGSSFYNPSLKSR Immunoglobulin mu heavy chain variable region (100%)

97. GLEWVSAVSGSGTTTYYADSUK Immunoglobulin mu heavy chain variable region (100%)

98. GLSDSVRSCR Thymocyte antigen CD1d (60%)

99. KAPQVYTIPPKE Anti-colorectal carcinoma heavy chain (100%)

100. KAPQVYTIPPKEQMAKD Anti-colorectal carcinoma heavy chain (100%)

101. KCELNCQAMGYR Immunoglobulin A heavy chain variable region (58%)

102. KNTQPIMDTDGSYFVYSKL Immunoglobulin heavy chain (100%)

103. KSSGTSYPDVLKC T cell receptor delta chain variable (61%)

104. KVTCVVVDISKD Hepatitis B virus receptor binding protein (75%)

10 Immunoglobulin heavy chain (75%)

Anti-colorectal carcinoma heavy chain (100%)

105. RLGEDNINVVEGNEQFISASKS T cell receptor variable alpha chain (50%)

106. LSSVTAADTNVYYCAR Immunoglobulin mu heavy chain variable region (100%)

107. RSVSELPIMHQDWLNGKE Hepatitis B virus receptor binding protein (100%)

108. RTLYLQMNSLR Immunoglobulin epsilon heavy chain variable region (90%)

109. SLPGSPKDSSHLLSPLR Immunoglobulin heavy chain (VH4) variable region (41%)

110. SLVVAAVAPDNRNPAFTTMGWLFLK Immunoglobulin heavy chain variable region (52%)

B-cell receptor CD22 isoform 3 precursor (40%)

111. TAKGSTGMEILLSTLENTK Immunoglobulin A heavy chain variable region (94%)

112. TVFSDSQLSGLEK MHC class II transactivator (92%)

T-cell receptor alpha chain V region-human (fragment) (46%)

113. APTVVLMMTK Protein tyrosine kinase, receptor type, D (80%)

MHC class II antigen (80%)

114. ATSRGCITIIGGGDTATCCAK Ephrin type-A receptor 5 (47%)

11 Mucin-17 protein (33%)

115. GPLCGCCPGRSSQK Interleukin-4 receptor alpha chain (64%)

Immunoglobulin variable region (57%)

116. KAPSILSVLK Interleukin-12 receptor, beta 1, isoform CRA_b (90%)

117. KDVLTITLTPKV Truncated soluble vascular endothelial growth factor receptor

Immunoglobulin lambda-light chain variable region (75%)

118.KGHQDSCPFELTACPNEGCTSQVPR TNF receptor-associated factor 6 (68%)

119. KVCNYVSWIKQ CDC20-like protein, isoform CRA_g (81%)

120. LSGSCRSTDSLHPCPPTALPR Microtubule-actin crosslinking factor 1, isoform CRA_i (80%)

Mucin-3B, partial (80%)

121. MAYLQQTLAGPSGTR TNF receptor-associated factor 1 isoform b (40%)

Immunoglobulin kappa chain variable region (53%)

122. MSSNCTSTTAVAVAPLSASK TNF receptor superfamily , member 11a precursor variant (95%)

Mucin-19-like (100%)

12 123. RVNSAAFPAPIEKT Mutant anaplastic lymphoma receptor tyrosine kinase (71%)

124. TLSGSAALWAESEK Ephrin type-A receptor 10 isoform 3 (90%)

Immunoglobulin rearranged L-chain V-region (57%)