Table S1 Summary of amino acid sequence homology analysis by NCBI Protein Blast Program with 124 tryptic peptides identified from CA215 by MALDI-TOF MS Amino Acid Sequence of Tryptic Peptides Sequence Homology Searched (%) 1. AAAGGLAMLTSMRPTLCSR Immunoglobulin heavy chain variable region (68%)
ICAM-3 (36%)
2. ADQGSETPSCLEVQCWGSR CD55 antigen, decay accelerating factor for complement (Cromer blood group), isoform CRA_j (52%)
Protocadherin alpha-10 isoform 3 precursor (36%)
3. AEALRTLTK Nestin (100%)
Microtubule-based motor (77%)
4. AFGSAAAFCWLCPCRCWK Adhesion molecule with Ig-like domain 1 (27%)
5. AGLLCHLDDACISNPCHK Delta and Notch-like epidermal growth factor-related receptor precursor (72%)
Dynein heavy chain 14, axonemal isoform 1 (83%)
Cadherin, EGF LAG seven-pass G-type receptor 1 (flamingo homolog, Drosophila), isoform CRA_a (66%)
6. ALACSGMKIGR ligatin, isoform CRA_c (54%)
TCR (72%)
MHC class I antigen (90%)
7. ANESGLAMTDISSLASQNK Melanoma associated antigen (mutated) 1 (36%) Tubulin gamma-1 chain (57%)
1 Cathepsin F (63%)
8. ANPQCMGQNHK Prolow-density lipoprotein receptor-related protein 1 precursor (90%)
9. APQLLCYAATNLQSGGPSR Anti-tetanus toxoid immunoglobulin light chain variable region (100%)
10. APTVVLHMTK Ig heavy chain variable region, VH3 family (70%)
Mucin 5AC protein (70%)
NF-IL6-beta protein (80%) 11. AQCVVSLAPR Interleukin-1 receptor accessory protein isoform 3 (50%)
MEGF10 protein, isoform CRA_b (100%)
Obscurin (80%)
12. ATVASGISPGWMGLDCGPESNK Circulating B cell antibody heavy chain variable region (59%)
Immunoglobulin E heavy chain variable region (50%)
Multiple epidermal growth factor-like domains protein 11 precursor (40%)
13. ATVMGSKCHR Mucin-16 (70%)
14. AVMNGLAPGSNGQDKDMDLTK Cellular myosin heavy chain (38%)
Fc receptor-like A (100%)
15. AVPSAEPQAGSPMTLSCQTK Leukocyte immunoglobulin-like receptor subfamily A member 2 soluble isoform 2 (85%) 16. CDVLK WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2, isoform CRA_b (100%) 17. CNGLMTIVFEENSKVTVPHMIK Immunoglobulin lambda chain variable region (59%)
2 18. DGLLYDTLVLINLESCDKK Nuclear receptor ROR-gamma isoform b (47%)
19. DHPYISEIK Fat-like cadherin FATJ protein (77%)
20. EAHFVRTSGK neural cell adhesion molecule 1, isoform CRA_h (70%)
21. EEAGKEEPPPLTPPAR T-box 3 (ulnar mammary syndrome), isoform CRA_d (100%)
22. EGAAGAFLPACK Multiple epidermal growth factor-like domains protein 8 (66%)
Immunoglobulin heavy chain variable region (58%)
23. EGGTQKTSTLPAVLYSCGICK Immunoglobulin heavy chain variable region (28%)
24. EMTDATNEEETEIKEDTK Ryanodine receptor 2 (cardiac), isoform CRA_c (77%)
25. GDGTSQGCAVPVESDPRHPR Pro-epidermal growth factor isoform 3 preproprotein (65%)
Titin (90%)
26. GDRVTITWR Immunoglobulin kappa chain variable region (100%)
Immunoglobulin light chain variable region (77%)
27. GELSPGHFLSGFSAAGASV Ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin), isoform CRA_b (31%)
28. GLCASAPFSCCLEQDVSRK T cell receptor beta variable 19 (36%)
Immunoglobulin mu heavy chain (63%)
29. GLEWVSAVSGSGTTTYYADSVK Immunoglobulin heavy chain variable region (100%)
Immunoglobulin E heavy chain variable region
3 (100%)
Immunoglobulin alpha heavy chain variable region (100%)
Anti-GM2 glanglioside IgM heavy chain variable region (100%)
30. GQPTYQICPRIIPTTESSCR NWD1 protein (30%)
31. GSSGSSGKCETK N.A.
32. GTEGLCPLLCHADGTPLGAGAR Sialic acid-binding Ig-like lectin 6 isoform 6 precursor (63%)
CD33L1 [Homo sapiens] >gb|AAK83655.1| sialic acid binding immunoglobulin-like lectin 6 (63%)
CD33L2 (63%)
Latent TGF-beta binding protein-4 (31%) 33. HESHRAVEFYQVVCVVFVFYYAK Receptor-type tyrosine-protein phosphatase beta isoform a (34%)
Protein tyrosine phosphatase, receptor type, B (34%)
34. HIISHSGNNPYGCEECGK CLL-associated antigen KW-4 splice variant 2 (94%)
35. IGTMVEDHR Titin (100%)
Complement component C8 beta chain preproprotein (55%)
36. KGGHSSPASSDK Mast cell-expressed membrane protein (75%)
37. KGYIPSYLDK Thyroid hormone receptor beta isoform (100%)
Immunoglobulin kappa 1 light chain (70%)
38. KHPSTVQVK T-cell leukemia translocation altered gene, isoform CRA_b (88%)
4 B-cell receptor CD22 isoform 3 precursor (77%)
V-set and immunoglobulin domain-containing protein 10-like (88%)
39. KTDQMVFHLETNSNSESHDK Protein tyrosine phosphatase, non-receptor type 20A (55%)
Breast cancer-associated antigen SGA-72M (60%)
40. LEPGNCKADETASK Protein-coupled receptor 112 (100%)
Cancer antigen 1 (71%)
41. LGLRCVTCNYCSQLCK Deformed epidermal autoregulatory factor 1 variant (93%)
42. LNTPTDSTLWVSLCCPR Titin isoform novex-2 (64%)
CDC42 binding protein kinase alpha (DMPK-like) (52%)
43. LSPLESRPPCPAGLVR Immunoglobulin kappa light chain variable region (100%)
Carbohydrate (keratan sulfate Gal-6) sulfotransferase 1, isoform CRA (68%)
Discoidin domain receptor family, member 1 (56%)
Receptor tyrosine kinase (56%)
44. LSTGVFGGGTK Immunoglobulin lambda light chain VLJ region (100%)
Immunoglobulin light chain variable region (100%)
Immunoglobulin V lambda/J lambda light chain (90%)
45. MAYLDQTLAGPSGTR Immunoglobulin kappa chain variable region (53%)
46. MCSCPISSPGHIKGLGQEETPVR Interleukin 23 receptor (30%)
5 Kainate receptor subunit KA2a (65%)
Glutamate receptor subunit (47%)
47. MIDSVMSCQQLTGLK Dynein heavy chain 2, axonemal (93%)
Dynein heavy chain domain 3, isoform CRA_c (86%)
48. MMGSTHIYDMSTVMSR Progestin and adipoQ receptor family member III, isoform CRA_b (50%)
49. MPIMLRSSNCVLTGK Vascular endothelial growth factor D preproprotein (73%)
T cell receptor alpha-chain V-region (V-J-C) precursor (73%)
50. MSLEWLVAWSWSLDGLR CD19 antigen, isoform CRA_c (52%)
Immunoglobulin heavy chain variable region (76%)
51. MSLLHKHQESGCSGCPR Relaxin receptor 2 isoform 1 (35%)
52. MSPSLFLPAMKEGCNAYK Nebulin isoform 2 (94%)
53. MSSNCTSTTAVAVAPLSASK Ig heavy chain (40%)
Immunoglobulin heavy chain variable region (50%)
Mucin-19-like (100%)
54. MSSPGPSQPPAEDPPWPAR Myopodin protein (84%)
55. MSTMRLLTLALLFSCSVAR Fibroblast growth factor receptor 4, isoform CRA_a (47%)
56. MSTRYHQAASDSYLELLK Dynein, axonemal, heavy chain 11 (100%)
57. MVKSYFCYAEQELMK Insulin-like growth factor 2 mRNA binding protein 1,
6 isoform CRA_b (46%)
58. MVMHARSGGNLEVMGLMLGK Receptor-type protein tyrosine phosphatase beta, RPTP beta, PTP zeta [human, brainstem, Peptide, 2307 aa] (45%）
Receptor-type tyrosine-protein phosphatase U isoform 2 precursor (65%)
Interleukin-20 receptor subunit beta precursor (70%)
59. NTDEPMGITLKMLNHCIVAR 55 kDa erythrocyte membrane protein isoform 4 (70%)
60. PATAPSCPSGSR Epidermal growth factor receptor isoform a variant (66%)
Mucin 5 (83%)
61. SAEPRGGVQVTTQQPR Cingulin (56%)
62. SEMVLYIQSEPVSQDAKPTGHNR Cell adhesion kinase beta (30%)
63. SESEEALPLDLDKNIHTPVSRPNR Major histocompatibility complex, class I, E (83%)
64. SGRPEELVSCADCGRSGGSGR Retinoid X receptor, alpha, isoform CRA_a (71%)
65. SISRPSSLRMSR Breast cancer nuclear receptor-binding auxiliary protein (66%)
66. SIVHPSYNSNTLNNDIMLIK Cathepsin 6 preprotein (75%) 67. SLSCESLWPIEAR Latrophilin-3 precursor (92%) 68. SMPTNCVWLDGLSSNVSDQYLTR Vascular endothelial growth factor receptor 1 isoform 4 precursor (73%)
Dynein heavy chain domain 1 isoform 1 (39%)
Cadherin-7 (56%)
Neuronal cell adhesion molecule isoform E precursor
7 (47%)
Immunoglobulin heavy chain (56%)
69. SPAQYQVVLSERLQALVNSLCAGQSP Tumor necrosis factor receptor superfamily member EDAR precursor (46%)
Trabeculin-alpha (100%)
70. SSGTSYPDVLK Immunoglobulin heavy chain variable region (100%)
Immunoglobulin gamma 1 heavy chain variable region (72%)
71. STNLPRNPSMADYEAR Protocadherin-8 isoform 2 precursor (50%)
72. TAAEADMDAAAGPPVSTRQSK Nuclear receptor co-repressor (100%)
73. TAKGSTGMEILLSTLENTK Immunoglobulin A heavy chain variable region (94%)
Immunoglobulin heavy chain variable region VH (78%)
Immunoglobulin gamma 3 heavy chain variable region (94%）
74. TAKPASQEHARCSAIGTGESPK Profilaggrin (86%) 75. TATIIIHANASMSHTILLK Cadherin-related family member 4 precursor (36%)
76. TGDPSPCPTPSHFDGVCDISCCEVK Mucin-2 precursor (96%) 77. TRTSFTAEQLYR Coronin, actin binding protein, 1B, isoform CRA_c (58%)
78. TSGATQTTVGDALFTR MHC class I antigen (56%)
Interleukin-9 (50%)
79. TSTGAVRMQPK Immunoglobulin gamma 2 heavy chain variable region (81%)
80. TVAMAGVAAMTAVTR HLA-B associated transcript 3 (100%)
8 81. TVCVLSERR Protocadherin Fat 4 precursor (66%)
82. TVGVIVSREAMTGR Cadherin-like 23 (71%)
Interleukin-1 receptor associated kinase-2 (57%)
83. TYFCQQSYTTPLTFGGGTK Immunoglobulin kappa light chain variable region (100%)
Immunoglobulin light chain variable region (100%)
IgM monoclonal antibody light chain variable region (100%)
84. VCHSNLIESR B-cell lymphoma 6 protein isoform 2 (50%)
Olfactory receptor, family 52, subfamily E, member 8 (100%)
85. VHTGEKPYGCSECEKNLGK Zinc finger protein 614 (100%)
86. VSSHSSQATKDSGVGLK Immunoglobulin epsilon heavy chain variable region (41%)
87. YLAAAFPSACGK Activin A receptor, type IB, isoform CRA_c (50%)
88. APAHHPAAISTAK MHC class I antigen (38%)
89. APWWSSCRWR Leukocyte immunoglobulin-like receptor subfamily B member 5 (50%)
Carcinoembryonic antigen-related cell adhesion molecule 8 precursor (100%)
90. AEDTAVYYCAKJLTIR Immunoglobulin heavy chain variable region (100%)
91. AELGGLLSPR Immunoglobulin heavy chain subgroup VIII V-D-J region (70%)
9 92. AETLVFSTHAVISMR Immunoglobulin heavy chain variable region (66%)
93. DGSISILGSDDATTCHIVVLR Colony stimulating factor 1 receptor (61%)
Interleukin 1 recepter-like 2 (47%)
Immunoglobulin heavy chain variable region (33%)
94. GDRVTITWR Immunoglobulin kappa chain variable region (100%)
95. GGNSGGSSSICYVLLGFIGTSK T cell receptor beta variable 7 (68%)
Immunoglobulin heavy chain variable region (40%)
96. GLECIGYMYSSGSSFYNPSLKSR Immunoglobulin mu heavy chain variable region (100%)
97. GLEWVSAVSGSGTTTYYADSUK Immunoglobulin mu heavy chain variable region (100%)
98. GLSDSVRSCR Thymocyte antigen CD1d (60%)
Immunoglobulin heavy chain variable region (70%)
99. KAPQVYTIPPKE Anti-colorectal carcinoma heavy chain (100%)
Immunoglobulin heavy chain (66%)
100. KAPQVYTIPPKEQMAKD Anti-colorectal carcinoma heavy chain (100%)
Immunoglobulin heavy chain (88%)
101. KCELNCQAMGYR Immunoglobulin A heavy chain variable region (58%)
102. KNTQPIMDTDGSYFVYSKL Immunoglobulin heavy chain (100%)
103. KSSGTSYPDVLKC T cell receptor delta chain variable (61%)
104. KVTCVVVDISKD Hepatitis B virus receptor binding protein (75%)
10 Immunoglobulin heavy chain (75%)
Anti-colorectal carcinoma heavy chain (100%)
105. RLGEDNINVVEGNEQFISASKS T cell receptor variable alpha chain (50%)
106. LSSVTAADTNVYYCAR Immunoglobulin mu heavy chain variable region (100%)
107. RSVSELPIMHQDWLNGKE Hepatitis B virus receptor binding protein (100%)
Immunoglobulin heavy chain (100%)
108. RTLYLQMNSLR Immunoglobulin epsilon heavy chain variable region (90%)
Immunoglobulin heavy chain variable region (100%)
109. SLPGSPKDSSHLLSPLR Immunoglobulin heavy chain (VH4) variable region (41%)
110. SLVVAAVAPDNRNPAFTTMGWLFLK Immunoglobulin heavy chain variable region (52%)
B-cell receptor CD22 isoform 3 precursor (40%)
111. TAKGSTGMEILLSTLENTK Immunoglobulin A heavy chain variable region (94%)
112. TVFSDSQLSGLEK MHC class II transactivator (92%)
T-cell receptor alpha chain V region-human (fragment) (46%)
113. APTVVLMMTK Protein tyrosine kinase, receptor type, D (80%)
MHC class II antigen (80%)
Immunoglobulin heavy chain variable region (70%)
114. ATSRGCITIIGGGDTATCCAK Ephrin type-A receptor 5 (47%)
Immunoglobulin heavy chain variable region (57%)
11 Mucin-17 protein (33%)
115. GPLCGCCPGRSSQK Interleukin-4 receptor alpha chain (64%)
Immunoglobulin variable region (57%)
116. KAPSILSVLK Interleukin-12 receptor, beta 1, isoform CRA_b (90%)
117. KDVLTITLTPKV Truncated soluble vascular endothelial growth factor receptor
Anti-colorectal carcinoma heavy chain (100%)
Immunoglobulin lambda-light chain variable region (75%)
118.KGHQDSCPFELTACPNEGCTSQVPR TNF receptor-associated factor 6 (68%)
Immunoglobulin heavy chain variable region (32%)
119. KVCNYVSWIKQ CDC20-like protein, isoform CRA_g (81%)
Immunoglobulin heavy chain variable region (90%)
120. LSGSCRSTDSLHPCPPTALPR Microtubule-actin crosslinking factor 1, isoform CRA_i (80%)
Immunoglobulin light chain variable region (38%)
Mucin-3B, partial (80%)
121. MAYLQQTLAGPSGTR TNF receptor-associated factor 1 isoform b (40%)
Immunoglobulin kappa chain variable region (53%)
122. MSSNCTSTTAVAVAPLSASK TNF receptor superfamily , member 11a precursor variant (95%)
Mucin-19-like (100%)
Immunoglobulin heavy chain variable region (50%)
12 123. RVNSAAFPAPIEKT Mutant anaplastic lymphoma receptor tyrosine kinase (71%)
Anti-colorectal carcinoma heavy chain (100%)
Immunoglobulin heavy chain (64%)
124. TLSGSAALWAESEK Ephrin type-A receptor 10 isoform 3 (90%)
Immunoglobulin rearranged L-chain V-region (57%)
13