Additional File 3. Figures of Catalytic Motifs and Active Sites of Selected Protease Families

Additional file 3. Figures of catalytic motifs and active sites of selected protease families

Figure S1. A22 protease family catalytic motif (active site shown in black)

....|....|....|....|....|....|....|....|....|...... |....|....|....|....|.... 460 470 480 490 680 690 700 Schistosoma_155880 LNNFLNNQNVRH----TFFINQ-----VDDLRFNRGALLNVGALESLEA TLVG----GMSNSFWGWGREDDEFQIRLK C_elegans_IMP3 [Q18408] SGNWISNDILAFASIY-VVCR------IQAVSYQTAIIFVIGMSLFDLF QSLAALDIMVPGVFLNVVLKYSSMYDTNL Schistosoma_154770 IG-WPLQSVIGM------FIAVIISSALIIPSVKVGTLLFTVFMIYDIF SLLGFGDAVIPGIFIQFLAFYDAC-WRTP C_elegans_IMP2 [P49049] ---WITNNIIGV-----SFSLGI--ERLHLASFKAGSLLLVGLFFYDIF SMLGLGDIVIPGIFIALLRRFDYRVVQTT Human_HM13 [Q8TCT9] ---WIANNLFGL-----AFSNGV--ELLHLNNVSTGCILLGGLFIYDVF AMLGLGDVVIPGIFIALLLRFDISLKKNT Mouse_HM13 [Q9D8V0] ---WIANNLFGL-----AFSNGV--ELLHLNNVSTGCILLGGLFIYDIF AMLGLGDIVIPGIFIALLLRFDISLKKNT Human_SPPL3 [Q8TCT6] ---WLLMDALAM-----GLCAMI--AFVRLPSLKVSCLLLSGLLIYDVF SMLGIGDIVMPGLLLCFVLRYDNYKKQAS Mouse_SPPL3 [Q9CUS9] ---WLLMDALAM-----GLCAMI--AFDRLPSLKVSCLLLSGLLIYDVF SMLGIGDIVMPGLLLCFVLRYDNYKKQAS C_elegans_IMP1 [Q93346] A--FILLDVINM-----ALCHVL--KCLRLPSLKWISILMLCMFVYDAF TILGLGDIVMPGYLVAHCFTMNGFSERVR Human_SPPL2A [Q8TCT8] WA-WILQDILGI-----AFCNLI--KTLKLPNFKSCVILLGLLLLYDVF SILGFGDIIVPGLLIAYCRRFDVQTGSSY Mouse_SPPL2A [Q9JJF9] WA-WILQDILGI-----AFCNLI--KTMKLPNFMSCVILLGLLLIYDVF SVLGFGDIIVPGLLIAYCRRFDVQTGSSI Human_SPPL2B [Q8TCT7] WA-WVLQDALGI-----AFCYML--KTIRLPTFKACTLLLLVLFLYDIF SLLGFGDILVPGLLVAYCHRFDIQVQSSR Mouse_SPPL2B [Q8BXJ5] WA-WVLQDTLGI-----AFCYML--KTIRLPTFKACTLLLLVLFIYDIF SLLGFGDILVPGLLVAYCHRFDIQVQSSR Human_SPPL2C [Q8IUH8] WA-WLLQDTLGI-----SYCFVL--HRVRLPTLKNCSSFLLALLAFDVF SILGFGDIVVPGFLVAYCCRFDVQVCSRQ Mouse_SPPL2B [A2A6C4] WA-WLLQDTLGV-----AYCFVL--RRVRLPTFKNCTLFLLALLAFDVF SILGFGDIVVPGFLVAYCHRFDMQVQSRQ C_elegans_SPE4 [Q01608] KTTWFVLWIVLFWDLFAVLAMGPLKKVQEKASDYSKCVLNLIMFSANEK LRLGFGDFVFYSLLIGQAAASGCPFAV-- C_elegans_HOP1 [O02100] WTVWMALTAISFWDIVAVLTCGPLKMLVETANRRGDDKFPAILYNSSSY IRLGMGDFVFYSLMLGNTVQT--CPLPTV Schistosoma_153960 WTCWLVLAALSVWDIVAVLCNGPLRLLVEMAHERQQPLFPALLYSTTTV VKLGLGDFVFYSLLIGRATLD--GDAVTV C_elegans_SEL12 [P52166] WTVWFVLFVISVWDLVAVLTKGPLRYLVETAQERNEPIFPALIYSSGVI VKLGLGDFIFYSVLLGKASSY--FDWNTT Human_PSEN2 [P49810] WSAWVILGAISVYDLVAVLCKGPLRMLVETAQERNEPIFPALIYSSAMV VKLGLGDFIFYSVLVGKAAATGSGDWNTT Mouse_PSEN2 [Q61144] WSAWVILGAISVYDLVAVLCKGPLRMLVETAQERNEPIFPALIYSSAMV VKLGLGDFIFYSVLVGKAAATGNGDWNTT Human_PSEN1 [P49768] WTAWLILAVISVYDLVAVLCKGPLRMLVETAQERNETLFPALIYSSTMV VKLGLGDFIFYSVLVGKASATASGDWNTT Mouse_PSEN1 [P49769] WTAWLILAVISVYDLVAVLCKGPLRMLVETAQERNETLFPALIYSSTMV VKLGLGDFIFYSVLVGKASATASGDWNTT Figure S2. C14 protease family (caspase) catalytic motif (active site shown in black)

|....|....|....|....|....|....|....|....|....|....|....|....|....|....| 135 145 155 165 175 185 195 Fruit fly_DRONC [Q9XYf4] TECFVMVLMTHGNSVE---GKEKVEFCDGSVV-DMQKIKDHFQTAKCPYLVNKPKVLMFPFCRGDEYDL-G Human_CASP1 [P29466] SDSTFLVFMSHGIREG---ICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGV-- Mouse_CASP1 [P2952] SDSTFLVFMSHGIQEG---ICGTTYSNEVSDILKVDTIFQMMNTLKCPSLKDKPKVIIIQACRGEKQGV-V Mouse_CASP12 [O08736] SDSTFLVFMSHGILEG---ICGVKHRNKKPDVLHDDTIFKIFNNSNCRSLRNKPKILIMQACRGRYNGT-- Mouse_CASP4 [P70343] SDSTFLVLMSHGTLHG---ICGTMHSEKTPDVLQYDTIYQIFNNCHCPGLRDKPKVIIVQACRGGNSGE-- Mouse_CASP11 [NP_031635] SDSTFLVLMSHGTLHG---ICGTMHSEKTPDVLQYDTIYQIFNNCHCPGLRDKPKVIIVQACRGGNSGE-- Human_CASP4 [P49662] SDSTFLVLMSHGILEG---ICGTVHDEKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGANRGE-- Human_CASP5 [P51878] SDSTFLVLMSHGILEG---ICGTAHKKKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGEKHGE-- Human_CASP2 [P42575] TDSCIVALLSHGVE------GAIYGVDGKLL-QLQEVFQLFDNANCPSLQNKPKMFFIQACRGDETDR-G Mouse_CASP2 [P29594] TDSCVVALLSHGVE------GGIYGVDGKLL-QLQEVFRLFDNANCPSLQNKPKMFFIQACRGDETDR-G Schistosoma_141270 YDCLVICLMSHGTI------GRIYGVDGNSL-SIHELTSIFTADNCPSLAGKPKLFFIQACRGEDYQK-G Schistosoma_032000 VHAAVLIILAHGLE------HHIIASDGIHV-SIDELVGCFTNKRCPLLAGKPKLILIQACRGEERNHNG Human_CASP14 [P31994] VSCAFVVLMAHGRE------GFLKGEDGEMV-KLENLFEALNNKNCQALRAKPKVYIIQACRGEQRDP-G Fruit fly_DREDD [ABI74776] -DSLVVFILSHGFE------EAVYASNSIAM-KITDIEDLLCSYD--TLYYKPKLLIIQACQEKLVHK-- A_aegyptii_DREDD [Q8IRY7] HCSLVICLLSHGQE------GKVYGSNSIPV-SVKAIERKMAARK---LTGKPKLLFVQACQGSGLQT-A Fruit fly_DAMM [Q9NBW3] -AGFVLFILSHGDRK------EKILACDHREY-HLDD-DVLFPLFRNPTLSGKPKILIVQACKGPLRAD-- Fruti fly_STRICTA [Q7KHK9] KSALVLVILSHGTRH------DQIAAKDDDYSLDDDVVFPILRNR---TLKDKPKLIFVQACKGDCQLG-G Human_CASP10 [Q92851] GDCFVFCILTHGRF------GAVYSSDEALI-PIREIMSHFTALQCPRLAEKPKLFFIQACQGEEIQP-S Human_CASP9 [P55211] LDCCVVVILSHGCQASHLQFPGAVYGTDGCPV-SVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDH-G Mouse_CASP9 [NP_056458] LDCFVVVILSHGCQASHLQFPGAVYGTDGCSV-SIEKIVNIFNGSGCPSLGGKPKLFFIQACGGEQKDH-G Human_CASP8 [Q14790] MDCFICCILSHGDK------GIIYGTDGQEA-PIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQK-G Mouse_CASP8 [O89110] KDCFICCILSHGDK------GVVYGTDGKEA-SIYDLTSYFTGSKCPSLSGKPKIFFIQACQGSNFQK-G Schistosoma_172010 HDCFACVILSHGDEG------GLIYATDGSI--PVDRIIAPFRGDQCLDLRGKPKLFFIQACRGMALDD-G Fruit fly_CASP1 [NP_524551] SDCILVAILSHGEM------GYIYAKDTQY--KLDNIWSFFTANHCPSLAGKPKLFFIQACQGDRLDG-G Fruit fly_DC[1 [NP_426974] NDCLAVAILSHGEH------GYLYAKDTQY--KLDNIWHYFTATFCPSLAGKPKLFFIQACQGDRLDG-G Human_CASP6 [P55212] ADCFVCVFLSHGEG------NHIYAYDAKI--EIQTLTGLFKGDKCHSLVGKPKIFIIQACRGNQHDVPV Mouse_CASP6 [O08738] ADCFICVFLSHGEG------NHVYAYDAKI--EIQTLTGLFKGDKCQSLVGKPKIFIIQACRGSQHDVPV Schistosoma_028500 YDSFIFVMLSHGDN------NIIYANDGEVLTSY--IMAFFRGDRCPSLIAKPKLFFFQACRGAAFDK-G Human_CASP7 [P55210] AACFACILLSHGEE------NVIYGKDGVT--PIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDD-G Mouse_CASP7 [P77864] SACFACVLLSHGEE------DLIYGKDGVT--PIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDD-G Human_CASP3 [P42574] RSSFVCVLLSHGEE------GIIFGTNGPV--DLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDC-G Mouse_CASP3 [70677] RSSFVCVILSHGDE------GVIYGTNGPV--ELKKLTSFFRGDYCRSLTGKPKLFIIQACRGTELDC-G C_elegans_CED3 [P42573] -DSAILVILSHGEE------NVIIGVDDIPI-STHEIYDLLNAANAPRLANKPKIVFVQACRGERRDN-G C_elegans_CSP1 [O18203] -DSIILFLLSHGDGA------GSVFGIDDMPV-NVMEVSTYL--AYHQNLLLKPKWVAVSACRGGKLNM-G C_elegans_CSP2 [Q9ZP5] -DSLIITIMSHGDQ------GLLYGVDGVPV-QMLDIIDLMCTA---SLAKKPKWLMCVCCRGDRIDR-A C_elegans_CSP3 [Q9XWE0] ------IDNFFDRI------PKFFQFMKSK------

Figure S3. M50 protease family catalytic motifs (active site shown in black)

|....|....|....|....|....|....|....|....|....|....|....|....| 265 275 285 295 305 315 325 Fruit fly_MBTPS2 [Q7JZ56] GVNLPLEEIGYYITTLVLCLVVHEMGHALAAVMEDVPVTGFGIKFIFCLPLAYTELSHDHL C_elegans_MBTPS2 [Q9U227] GFNLPWGHIPIFMLVLIVAAVFHELGHAWAATSNGVTVNGFGIFILAVYPGAFTDIEAVTL Schistosoma_054310 GINLPTSHLGFYALTLLICAFIHEAGHALAAVRERVRLHGFGIFVFGFYPGAFVDLNAADL Mouse_MBTPS2 [Q8CHX6] GINLPVNQLTYFFAAVLISGVVHEIGHGIAAIREQVRFNGFGIFLFIIYPGAFVDLFTTHL Human_MBTPS2 [O43462] GINLPVNQLTYFFTAVLISGVVHEIGHGIAAIREQVRFNGFGIFLFIIYPGAFVDLFTTHL Frog_MBTPS2 [A4IGW4] GVNLPISQLSYFFSAILISGVLHEVGHGVAAVRESVRFNGFGMFIFIVYPGAFVDLFTTHL Chicken_MBTPS2 [XP_425566] GVNLPVSQLTYFFSAILISGVIHEVGHGVAAIREQVRFNGFGIFIFIVYPGAFVDLFTTHL

Figure S4. M16 protease family (mitochondrial processing protease, subunit beta) catalytic motif (active site shown in black) ..|....|....|....|....|....|....|....|....| ....|....|....|....|... 115 125 135 145 155 185 195 Schistosoma_MPPB [009650.2] GVAHFLEHMAFKGTEKRSQQSLELEVEDKGAHLNAYTSREMTV LKNSKFESSQVERERGVILREME C_elegans_MPPB [Q23295] GTAHFLEHMAFKGTPRRTRMGLELEVENIGAHLNAYTSRESTT LLNSSLATKDIEAERGVIIREME Human_MPPB [O75439] GTAHFLEHMAFKGTKKRSQLDLELEIENMGAHLNAYTSREQTV IQNSTLGEAEIERERGVILREMQ Chimpanzee_MPPB [Q5REK3] GTAHFLEHMAFKGTKKRSQLDLELEIENMGAHLNAYTSREQTV IQNSTLGEAEIERERGVILREMQ Cow_MPPB [Q3SZ71] GTAHFLEHMAFKGTKKRSQLDLELEIENMGAHLNAYTSREQTV IQNSTLGEAEIERERGVILREMQ Mouse_MPPB [Q9CXT8] GTAHFLEHMAFKGTKKRSQLDLELEIENMGAHLNAYTSREQTV IQNSTLGEAEIERERGVILREMQ Frog_MPPB [Q0V9F0] GTAHFLEHMAFKGTKNRSQLDLELEIENMGAHLNAYTSREQTV IQNSTLGEAEIERERGVILREMQ A_thaliana_MPPB [Q42290] GTAHFLEHMIFKGTDRRTVRALEEEIEDIGGHLNAYTSREQTT LQNSKFEEQRINRERDVILREMQ S_cerevisiae_MPPB [P10507] GTAHFLEHLAFKGTQNRSQQGIELEIENIGSHLNAYTSRENTV LTKSVLDNSAIERERDVIIRESE Figure S5. S01 protease family catalytic motif (active site shown in black)

|....|....|....| .|....|....|. |....|....|.... 50 60 175 185 355 365 A_gambiae_SP24D [Q17004] GSLIESRWVLTAAHCV DVALLQL-QLSLP VN-NGACNGDSGGP- C_elegans_LAT2 [NP_001040724] GTGTAFAWIFTILNC- NMIKVNGHSYPST IERMSHASSDPRGS- Schistosoma_006510 GSLVSTRAVLTAGHC- DIATVML-AQMVN FG-QITAPGDSGGP- Schistosoma_119130 GSLVSTRAVLTAGHC- DIAIVML-AQMVN FG-QLPAPGDSGGP- Schistosoma_006520 GSLVSSRAVLTAGHC- DMAIITL-TNLVN SK-QIAGPGDSGGP- Schistosoma_112090 GSLVSTRAVLTAGHC- DIATVML-AQMVN FG-QITAPGDSGGP- C_elegans_TRY3 [NP_500999] ATVIDDFWLVTAAHC- DIALLRI-SSDLS -YLHGTAPGDSGGP- C_elegans_TRY4 [NP_508030] GSIISPYHIITAAHG- DWAIVEV-EKRIH YSAPRTCHGDSGGG- C_elegans_TRY7 [NP_491910] GTIVSPRHILIATHC- DFAIVHL-YEELT ASLNISLKGDSGGG- C_elegans_TRY6 [NP_491898] GTLTSPRHILTATHCA DIMIIEL-SEDVE TRTSVACPGDSGAGG Fruit fly_STUBBLE [Q05319] GALINENWIATAGHC- DLALVKL-EQPLE TGGQDSCQGDSGGP- Schistosoma_002150 GTLIAPQWILTAAHC- DIALLRL-SEPVK -KNKDTCAGDSGGG- Human_CFB [P00751] GAVVSEYFVLTAAHC- DVALIKL-KNKLK YADPNTCRGDSGGP- Human_C2 [P06681] GALISDQWVLTAAHC- DIALLKL-AQKVK QEDESPCKGESGGA- Schistosoma_030350 GSLISAQWVMTAAHC- DIALLRL-QTPAN QGGIDACQGDSGGP- Human_ELA1 [P08218] GSLIANSWVLTAAHC- DIALLKL-ANPVS DGVICTCNGDSGGP- Human_HGF [P14210] GSLIKESWVLTARQC- DLVLMKL-ARPAV KIGSGPCEGDYGGP- Human_PLG [P00747] GTLISPEWVLTAAHC- DIALLKL-SSPAV AGGTDSCQGDSGGP- Human_LPA [P08519] GTLISPEWVLTAAHC- DIALLKL-SRPAV ARGTDSCQGDSGGP- Human_DF [P00746] GVLVAEQWVLSAAHC- DLLLLQL-SEKAT SNRRDSCKGDSGGP- Human_GZMA [P12544] GALIAKDWVLTAAHC- DLKLLQL-TEKAK RGGRDSCNGDSGSP- Human_CMA1 [P23946] GFLIRRNFVLTAAHC- DIMLLKL-KEKAS RKTKSAFKGDSGGP- Human_CTSG [P08311] GFLVREDFVLTAAHC- DIMLLQL-SRRVR RERKAAFKGDSGGP- Human_GRZB [P10144] GFLIQDDFVLTAAHC- DIMLLQL-ERKAK EIKKTSFKGDSGGP- Human_AZU1 [P20160] GALIHARFVMTAASC- DLMLLQL-DREAN TRRGGICNGDGGTP- Human_ELA2 [P08246] ATLIAPNFVMSAAHC- DIVILQL-NGSAT GRQAGVCFGDSGSP- Human_PRTN3 [P24158] GTLIHPSFVLTAAHC- DVLLIQL-SSPAN RRKAGICFGDSGGP- Schistosoma_103680 ASLISSQWLLTAAHC- DYALIKI-VSPIQ MGGKDSCQGDSGSP- C_elegans_TRY2 [NP_501379] ASILDKTHLITAAHC- DIAILEIPYPGIE EGGIDSCQGDSGGP- C_elegans_TRY1 [NP_494910] GSLIDPNFVLTAAHC- DFAIMRI-HPPVN YGKIDSCQGDSGGP- Human_PRSS8 [Q16651] GSLVSEQWVLSAAHC- DIALLQL-SRPIT EGGKDACQGDSGGP- Human_PRSS7 [P98073] ASLVSSDWLVSAAHC- DIAMMHL-EFKVN EGGIDSCQGDSGGP- Human_KLKB1 [P03952] GSLIGHQWVLTAAHC- DIALIKL-QAPLN EGGKDACKGDSGGP- Human_F11 [P03951] GSIIGNQWILTAAHC- DIALLKL-ETTVN EGGKDACKGDSGGP- Figure S6. S54 protease family (rhomboid) catalytic motif (active site shown in black)

|....|....|....|....|....|....|....|....|....|....| |....|....|.... 830 840 850 860 870 890 940 950 Fruit fly_RHOM6 [NP_788038] NAWL------QPHLHLM------GASAGVYAMLGSHVPHLVLNFSQLS-- NRNPRTSLEAHIGG— C_elegans_ROM1 [NP_498029] QYAI------DPNSLLV------GASAGVYALIFAHVANVILNWHEMP-- NDCDSVSHLAHIAG— Fruit fly_RHOM4 [NP_525084] TSLT------SPRIFLA------GASGGVYALITAHIATIIMNYSEME-- DQHDQIGYVAHLSG— Schistosoma_020090 HSVS------DPFVLLA------GASGGCYALIGAHLATVIMNWDIMQ-E DERTRVGFSAHFGG— Human_RHBDL2 [NP_060291] SSIF------DPLRYLV------GASGGVYALMGGYFMNVLVNFQEMI-- EDGSPVSFAAHIAG— Fish_RHBDL2 [NP_957498] SSIF------DPFSALV------GASGGVYALMGGYFMNAIVNFREMR-- EAGLKVSFVAHIGG— Human_RHBDL1 [NP_003952] VSIT------DMRAPVV------GGSGGVYALCSAHLANVVMNWAGMR-- ASGPQPSFMAHLAG— Mouse_RHBDL1 [NP_659065] VSIT------DMRAPVV------GGSGGVYALCSAHLANVVMNWAGMR-- ASGPQPSFMAHLAG— Fish_RHBDL3 [CAM16836] VSVT------DMTAPVV------GSSGGVYALVSAHLANVVMNWSGMK-- PPCPNPSFVAHLGG— Human_RHBDL3[NP_612201] VSVA------DMTAPVV------GSSGGVYALVSAHLANIVMNWSGMK-- PPCPHPSFVAHLGG— Mouse_RHBDL3 [NP_631974] VSVA------DMTAPVV------GSSGGVYALVSAHLANIVMNWSGMK-- PPCPHPSFVAHLGG— Fruit fly_RHOM2 [NP_788450] TSIF------DPDVFLV------GASGGVYALLAAHLANVLLNYHQMR-- ETAGAVSYVAHLAG— Fruit fly_RHOM1 [NP_52388] TSVV------DSEVFLV------GASGGVYALLAAHLANITLNYAHMK-- AKGPQVSYIAHLTG— Fruit fly_RHOM3 [NP_524790] TSVV------DSEVFLV------GASGGVYALLAAQLASLLLNFGQMR-- QTRPSVSYIAHMTG— S_cerevisiae_PARL [NP_011615] SLWYPKLARLAIVGPSL------GASGALFGVLG------CFSY--- LRWGSFDYAAHLGG— Schistosoma_032420 SLLN—-KLLRRSTFPSL------GASGGICAIIG------ALSML-- SRRSALDHAAHAGG— C_elegans_ROM5 [NP_491125] SIVD-KAVVRSPIRAL------GASGAILAALT------YTCMQ-- LRFRLFDHAAHLGG— Fruit fly_PARL [NP_523704] SVLY—-KAATSQAGMSL------GASGAIMTLLA------YVCTQ-- MGWKFFDHAAHLGG— Human_PARL [NP_061092] SYVG—-KVATGRYGPSL------GASGAIMTVLA------AVCTK-- LGWKFFDHAAHLGG— Mouse_PARL [NP_001005767] SYVC—-KVATGRYGPSL------GASGAIMTVLA------AVCTK-- LGWKFFDHAAHLGG— S_cerevisiase_RBD2 [NP_015078] CLLG---KLLYPEALVA------GASGWCFTLFAYYSFKESQIRPRTR-- ---PGSSFWGHFFG— C_elegans_RHBDF1 [NP_503031] SAIF------VPYYPTV------GPSSAQCGVFSSVVVELWHFRHLLD-P -----IDNWSHLFG— C_elegans_RHBDF2 [NP_001076719] SAIF------VPYNPAV------GPSSAQCGILAAVIVECCDNRRIIKEF -----VDNWAHLFG— Schistosoma_008620 SGIF------LPYQVET------GPTGAQFALLGISLVDLIHCWQFLA-H -----IDNYANAGS— Mouse_RHBDF2 [NP_766160] SAIF------LPYRAEV------GPAGSQFGLLACLFVELFQSWQLLE-R -----IDNIAHIFG— Human_RHBDF2 [NP_078875] SAIF------LPYRAEV------GPAGSQFGLLACLFVELFQSWPLLE-R -----IDNIAHIFG— Fish_RHBDF [NP_001002228] SAIF------LPYRAEV------GPAGSQFGILACLFVELIQSWQILA-Q -----IDNFAHISG— Human_RHBDF1 [NP_071895] SAIF------LPYRAEV------GPAGSQFGILACLFVELFQSWQILA-R -----IDNFAHISG— Mouse_RHBDF1 [NP_034247] SAIF------LPYRAEV------GPAGSQFGILACLFVELFQSWQILA-R -----IDNFAHISG— Fish_RHBDL4 [NP_001017614] LPIA------NRYACWVELVLIHIMNPGTSFV---GHLSGILVGLLYTT-G PYAPNTNYEQHYYGA Human_RHBDL4 [NP_115652] FPVP------NRFACWVELVAIHLFSPGTSFA---GHLAGILVGLMYTQ-G PHGRPDHYEEAPRN— Mouse_RHBDL4 [NP_084053] FPVP------NRFACWAELVAIHFCTPGTSFA---GHLAGILVGLMYTQ-G ADGRPVTYDATYRN—