Supplementary Figure 1: In situ hybridization of miR-21 in CC10-IL13 bitransgenic mouse lung. Expression of miR-21 in CC10-IL13 bitransgenic mouse lungs, were determined by LNA-based in situ hybridization. Left: 200X field, LNA- anti-miR-21. Right: serial sections at 600X; right top: LNA-anti-miR-21, right middle: anti-CD68, right bottom: LNA-scrambled control probe. miR-21 positive cells are indicated by red arrows.

Supplementary Figure 2: Cell counts of BALF cells in saline and Aspergillus fumigatus challenged mice. Data are represented as mean ± S.E.M. Supplementary Table I: IL-13 up-regulated with predicted NFIB binding sites in the 1KB region up-stream of 1st exon.

Gene Name Ensembl ID Description Acrv1 ENSMUSG00000032110 Acrosomal vesicle 1 A disintegrin-like and metallopeptidase (reprolysin type) with Adamts19 ENSMUSG00000053441 thrombospondin type 1 motif, 19 A disintegrin-like and metallopeptidase (reprolysin type) with Adamts4 ENSMUSG00000006403 thrombospondin type 1 motif, 4 Adra1a ENSMUSG00000045875 Adrenergic receptor, alpha 1a Agr2 ENSMUSG00000020581 Anterior gradient 2 (Xenopus laevis) Aldoc ENSMUSG00000017390 Aldolase 3, C isoform Anxa2 ENSMUSG00000032231 A2 Ascl2 ENSMUSG00000009248 Achaete-scute complex homolog-like 2 (Drosophila) Asf1b ENSMUSG00000005470 ASF1 anti-silencing function 1 homolog B (S. cerevisiae) Atf4 ENSMUSG00000042406 Activating transcription factor 4 B3galt4 ENSMUSG00000067370 UDP-Gal:betaGalNAc beta 1,3-galactosyltransferase, polypeptide 4 Btrc ENSMUSG00000025217 Beta-transducin repeat containing protein Cabc1 ENSMUSG00000026489 Chaperone, ABC1 activity of bc1 complex like (S. pombe) Camp ENSMUSG00000038357 Cathelicidin antimicrobial peptide Cd82 ENSMUSG00000027215 CD82 antigen Cd83 ENSMUSG00000015396 CD83 antigen Cdc2l6 ENSMUSG00000038481 Cell division cycle 2-like 6 (CDK8-like) Cdc6 ENSMUSG00000017499 Cell division cycle 6 homolog (S. cerevisiae) Cdca5 ENSMUSG00000024791 Cell division cycle associated 5 Cdk2ap1 ENSMUSG00000029394 CDK2 (cyclin-dependent kinase 2)-associated protein 1 Cfi ENSMUSG00000058952 Complement component factor i Cilp ENSMUSG00000042254 Cartilage intermediate layer protein, nucleotide pyrophosphohydrolase Cit ENSMUSG00000029516 Citron Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal Cited4 ENSMUSG00000070803 domain, 4 Clca2 ENSMUSG00000028262 Chloride channel calcium activated 2 Csf1 ENSMUSG00000014599 Colony stimulating factor 1 (macrophage) Cx3cr1 ENSMUSG00000052336 Chemokine (C-X3-C) receptor 1 Cxcl9 ENSMUSG00000029417 Chemokine (C-X-C motif) ligand 9 Cxcr6 ENSMUSG00000048521 Chemokine (C-X-C motif) receptor 6 Cycs ENSMUSG00000063694 Cytochrome c, somatic Cyp1a2 ENSMUSG00000032310 Cytochrome P450, family 1, subfamily a, polypeptide 2 Dag1 ENSMUSG00000039952 Dystroglycan 1 Ddx4 ENSMUSG00000021758 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 Dio2 ENSMUSG00000007682 Deiodinase, iodothyronine, type II Dlx3 ENSMUSG00000001510 Distal-less homeobox 3 Dmp1 ENSMUSG00000029307 Dentin matrix protein 1 Dnaja3 ENSMUSG00000004069 DnaJ (Hsp40) homolog, subfamily A, member 3 Dus4l ENSMUSG00000020648 Dihydrouridine synthase 4-like (S. cerevisiae) Ehf ENSMUSG00000012350 Ets homologous factor Enc1 ENSMUSG00000041773 Ectodermal-neural cortex 1 Enpp1 ENSMUSG00000037370 Ectonucleotide pyrophosphatase/phosphodiesterase 1 Fanca ENSMUSG00000032815 Fanconi anemia, complementation group A Fbxo44 ENSMUSG00000029001 F-box protein 44 Fcer1g ENSMUSG00000058715 Fc receptor, IgE, high affinity I, gamma polypeptide Fgf4 ENSMUSG00000050917 Fibroblast growth factor 4 Fgl2 ENSMUSG00000039899 Fibrinogen-like protein 2 Fn1 ENSMUSG00000026193 Fibronectin 1 Fosl2 ENSMUSG00000029135 Fos-like antigen 2 Foxf2 ENSMUSG00000038402 Forkhead box F2 Foxh1 ENSMUSG00000033837 Forkhead box H1 Foxm1 ENSMUSG00000001517 Forkhead box M1 Gadd45g ENSMUSG00000021453 Growth arrest and DNA-damage-inducible 45 gamma Gfi1b ENSMUSG00000026815 Growth factor independent 1B Glra1 ENSMUSG00000000263 Glycine receptor, alpha 1 subunit Gprc5c ENSMUSG00000051043 G protein-coupled receptor, family C, group 5, member C Grik2 ENSMUSG00000056073 Glutamate receptor, ionotropic, kainate 2 (beta 2) Habp2 ENSMUSG00000025075 Hyaluronic acid binding protein 2 Hoxc5 ENSMUSG00000022485 Homeo box C5 Hs6st3 ENSMUSG00000053465 Heparan sulfate 6-O-sulfotransferase 3 Hspa5 ENSMUSG00000026864 Heat shock protein 5 Icam4 ENSMUSG00000001014 Intercellular adhesion molecule 4, Landsteiner-Wiener blood group Id2 ENSMUSG00000020644 Inhibitor of DNA binding 2 Il1r2 ENSMUSG00000026073 Interleukin 1 receptor, type II Il6 ENSMUSG00000025746 Interleukin 6 Itga5 ENSMUSG00000000555 Integrin alpha 5 (fibronectin receptor alpha) Itih4 ENSMUSG00000021922 Inter alpha-trypsin inhibitor, heavy chain 4 Kcnj10 ENSMUSG00000044708 Potassium inwardly-rectifying channel, subfamily J, member 10 Lass6 ENSMUSG00000027035 Longevity assurance homolog 6 (S. cerevisiae) Lcn2 ENSMUSG00000026822 Lipocalin 2 Lgals1 ENSMUSG00000068220 Lectin, galactose binding, soluble 1 Lhx2 ENSMUSG00000000247 LIM homeobox protein 2 Lrrtm1 ENSMUSG00000060780 Leucine rich repeat transmembrane neuronal 1 Ly6h ENSMUSG00000022577 Lymphocyte antigen 6 complex, locus H Mab21l1 ENSMUSG00000056947 Mab-21-like 1 (C. elegans) Mab21l2 ENSMUSG00000057777 Mab-21-like 2 (C. elegans) V-maf musculoaponeurotic fibrosarcoma oncogene family, protein B Mafb ENSMUSG00000074622 (avian) Magel2 ENSMUSG00000056972 Melanoma antigen, family L, 2 Map4k1 ENSMUSG00000037337 Mitogen activated protein kinase kinase kinase kinase 1 Masp1 ENSMUSG00000022887 Mannan-binding lectin serine peptidase 1 Mfsd2 ENSMUSG00000028655 Major facilitator superfamily domain containing 2 Mtf2 ENSMUSG00000029267 Metal response element binding transcription factor 2 Mxd1 ENSMUSG00000001156 MAX dimerization protein 1 Nkx2-2 ENSMUSG00000027434 NK2 transcription factor related, locus 2 (Drosophila) Nmnat3 ENSMUSG00000032456 Nicotinamide nucleotide adenylyltransferase 3 Nov ENSMUSG00000037362 Nephroblastoma overexpressed gene Nr5a1 ENSMUSG00000026751 Nuclear receptor subfamily 5, group A, member 1 Ntn2l ENSMUSG00000079662 Netrin 2-like (chicken) Ntng2 ENSMUSG00000035513 Netrin G2 Ntrk3 ENSMUSG00000059146 Neurotrophic tyrosine kinase, receptor, type 3 Odf1 ENSMUSG00000061923 Outer dense fiber of sperm tails 1 Orm1 ENSMUSG00000039196 Orosomucoid 1 Pcdhgc4 ENSMUSG00000023036 Protocadherin gamma subfamily A Pdxp ENSMUSG00000068221 Pyridoxal (pyridoxine, vitamin B6) phosphatase Phkb ENSMUSG00000036879 Phosphorylase kinase beta Pipox ENSMUSG00000017453 Pipecolic acid oxidase Pitx3 ENSMUSG00000025229 Paired-like homeodomain transcription factor 3 Pml ENSMUSG00000036986 Promyelocytic leukemia Pou3f2 ENSMUSG00000052621 POU domain, class 3, transcription factor 2 Ppp1r3f ENSMUSG00000039556 Protein phosphatase 1, regulatory (inhibitor) subunit 3F Proteoglycan 4 (megakaryocyte stimulating factor, articular superficial Prg4 ENSMUSG00000006014 zone protein) Ptpn2 ENSMUSG00000024539 Protein tyrosine phosphatase, non-receptor type 2 Ptpn5 ENSMUSG00000030854 Protein tyrosine phosphatase, non-receptor type 5 Rad51 ENSMUSG00000027323 RAD51 homolog (S. cerevisiae) Rcor2 ENSMUSG00000024968 REST corepressor 2 Rnf182 ENSMUSG00000044164 Ring finger protein 182 Rsad2 ENSMUSG00000020641 Radical S-adenosyl methionine domain containing 2 S100a8 ENSMUSG00000056054 S100 calcium binding protein A8 (calgranulin A) Sall1 ENSMUSG00000031665 Sal-like 1 (Drosophila) Sall3 ENSMUSG00000024565 Sal-like 3 (Drosophila) Sall4 ENSMUSG00000027547 Sal-like 4 (Drosophila) Shh ENSMUSG00000002633 Sonic hedgehog Slamf9 ENSMUSG00000026548 SLAM family member 9 Slc16a4 ENSMUSG00000027896 Solute carrier family 16 (monocarboxylic acid transporters), member 4 Slc16a6 ENSMUSG00000041920 Solute carrier family 16 (monocarboxylic acid transporters), member 6 Solute carrier family 7 (cationic amino acid transporter, y+ system), Slc7a11 ENSMUSG00000027737 member 11 Smad2 ENSMUSG00000024563 MAD homolog 2 (Drosophila) Sox9 ENSMUSG00000000567 SRY-box containing gene 9 Stac ENSMUSG00000032502 Src homology three (SH3) and cysteine rich domain Syt12 ENSMUSG00000049303 XII Tbx21 ENSMUSG00000001444 T-box 21 Tes ENSMUSG00000029552 Testis derived transcript Thrsp ENSMUSG00000035686 Thyroid hormone responsive SPOT14 homolog (Rattus) Timp2 ENSMUSG00000017466 Tissue inhibitor of metalloproteinase 2 Ube2t ENSMUSG00000026429 Ubiquitin-conjugating enzyme E2T (putative) Ubtf ENSMUSG00000020923 Upstream binding transcription factor, RNA polymerase I Vcam1 ENSMUSG00000027962 Vascular cell adhesion molecule 1 Wdr12 ENSMUSG00000026019 WD repeat domain 12 Ybx2 ENSMUSG00000018554 Y box protein 2 Zfpm2 ENSMUSG00000022306 Zinc finger protein, multitype 2 Zic4 ENSMUSG00000036972 Zinc finger protein of the cerebellum 4