A Survey of Genes Encoding H2O2-Producing GMC Oxidoreductases in 10 Polyporales Genomes

Mycologia, 107(6), 2015, pp. 1105–1119. DOI: 10.3852/15-027 # 2015 by The Mycological Society of America, Lawrence, KS 66044-8897

A survey of genes encoding H2O2-producing GMC oxidoreductases in 10 Polyporales genomes

Patricia Ferreira1 Key words: brown-rot fungi, evolutionary relation- Departamento de Bioquímica y Biología Molecular y Celular ships, GMC oxidoreductases, sequenced genome analysis, and BIFI, Universidad de Zaragoza, E-50009 Zaragoza, white-rot fungi Spain INTRODUCTION Juan Carro1 Ana Serrano Although species from several Basidiomycota (and Angel T. Martínez2 some Ascomycota) orders contribute to lignocellulose Centro de Investigaciones Biológicas, CSIC, Ramiro de decay, the ability to degrade wood is a typical feature Maeztu 9, E-28040 Madrid, Spain of the order Polyporales. This capability was an essential evolutionary trait acquired by ancestral basidiomycetes in the later Carboniferous period (Floudas et al. Abstract: The genomes of three representative Poly- 2012), when the amount of carbon fixed by photo- porales (Bjerkandera adusta, Phlebia brevispora and a synthesis strongly increased due to colonization of member of the Ganoderma lucidum complex) recently land ecosystems by vascular plants. Nowadays fungal were sequenced to expand our knowledge on the diversity and distribution of genes involved in degradation of decay of wood represents a natural model for the sus- plant polymers in this Basidiomycota order, which tainable use of plant resources in lignocellulose biore- includes most wood-rotting fungi. Oxidases, including fineries (Martínez et al. 2009, Ragauskas et al. 2014). members of the glucose-methanol-choline (GMC) oxi- The first basidiomycete genome to be sequenced doreductase superfamily, play a central role in the was that of Phanerochaete chrysosporium (5 Phanerodontia above degradative process because they generate extra- chrysosporium) (Martinez et al. 2004) due to the interest cellular H2O2 acting as the ultimate oxidizer in both in this white-rot fungus of the order Polyporales as a white-rot and brown-rot decay. The survey was com- model lignin-degrading organism (Kersten and Cullen pleted by analyzing the GMC genes in the available 2007). Wood attack by white-rot fungi is based on their genomes of seven more species to cover the four Poly- ability to degrade the recalcitrant polymer of lignin in porales clades. First, an in silico search for sequences a process that was defined as an enzymatic “combus- encoding members of the aryl-alcohol oxidase, glucose tion” (Kirk and Farrell 1987) and combines extracellu- oxidase, methanol oxidase, pyranose oxidase, cello- lar oxidases and peroxidases (Kersten and Cullen biose dehydrogenase and pyranose dehydrogenase 2007, Ruiz-Dueñas and Martínez 2009). With a few families was performed. The curated sequences were exceptions corresponding to poor wood rotters (e.g. subjected to an analysis of their evolutionary relation- species of Jaapiales and Cantharellales), the presence ships, followed by estimation of gene duplication/ of lignin peroxidase (LiP3, EC 1.11.1.14), manganese reduction history during fungal evolution. Second, the molecular structures of the near one hundred peroxidase (MnP, EC 1.11.1.13) or versatile peroxidase GMC oxidoreductases identified were modeled to (VP, EC 1.11.1.16) genes is a constant characteristic of gain insight into their structural variation and expected all typical white-rot fungi based on comparative gen- catalytic properties. In contrast to ligninolytic peroxi- ome analysis (Floudas et al. 2012, 2015; Ruiz-Dueñas dases, whose genes are present in all white-rot Polypor- et al. 2013). The diversity, distribution and evolution- ales genomes and absent from those of brown-rot ary relationships of ligninolytic peroxidases in the species, the H2O2-generating oxidases are widely dis- order Polyporales has been studied (Ruiz-Dueñas et al. tributed in both fungal types. This indicates that the 2013). GMC oxidases provide H2O2 for both ligninolytic per- Brown-rot fungi have developed an alternative strat- oxidase activity (in white-rot decay) and Fenton attack egy, based on Fenton chemistry, to overcome the lig- on cellulose (in brown-rot decay), after the transition nin barrier (Baldrian and Valaskova 2008). H2O2 between both decay patterns in Polyporales occurred. reduction by ferrous iron yields hydroxyl free radical, which is able to access, oxidize and depolymerize wood cellulose with a more or less limited modification of lignin (Kirk 1975, Martínez et al. 2011, Yelle et al. Submitted 7 Feb 2015; accepted for publication 7 Jul 2015. 1 These two authors contributed equally to this paper. 2011). In 2009 the genome of Rhodonia placenta (syn.: 2 Corresponding author. E-mail: [email protected] Postia placenta) was sequenced as the model brown-rot

1105 1106 MYCOLOGIA fungus to increase our understanding of this type of (Ruiz-Dueñas et al. 2013, Hori et al. 2013, Mgbeahur- wood decay (Martinez et al. 2009). uike et al. 2013, Syed et al. 2013, Kovalchuk et al. Several oxidases have been related to wood biode- 2013) as an example of genome-enabled mycology to gradation as a source of extracellular H2O2 (from O2 gain insight into the biology and evolution of fungi reduction), including glucose oxidase (GOX, EC (Hibbett et al. 2013). 1.1.3.4) (Kelley and Reddy 1986), methanol oxidase (MOX, EC 1.1.3.13, also known as ethanol/alcohol MATERIALS AND METHODS oxidase) (Nishida and Eriksson 1987, Daniel et al. 2007), aryl-alcohol oxidase (AAO, EC 1.1.3.7) (Guillén Genome sequencing.—The genomic sequences of B. adusta et al. 1990), pyranose 2-oxidase (P2O, EC 1.1.3.10) (HHB-12826-SP), P. brevispora (HHB-7030-SS6) and Ganoderma (Daniel et al. 1992) and glyoxal oxidase (GLX, EC sp. (10597-SS1) were obtained at the Joint Genome Institute 1.1.3.−) (Kersten and Kirk 1987). Although the invol- (JGI), as part of the Saprotrophic Agaricomycotina Project vement of some other intracellular oxidases has been coordinated by D.S. Hibbett (Clark University, USA). The genomes were produced as described by Binder et al. (2013), and suggested (Greene and Gould 1984, Kelley and Reddy the gene prediction is available at http://genome.jgi.doe.gov/ 1986), wood decay is an extracellular process and Bjead1_1; http://genome.jgi.doe.gov/Gansp1 and http:// secreted oxidases are more likely involved. Alternative genome.jgi.doe.gov/Phlbr1, respectively. mechanisms for H2O2 generation have been suggested, including Mn(III)-mediated oxidation of Genome screening for GMC gene families in Polyporales.—The glyoxylic/oxalic acids (Urzúa et al. 1998). above genomes, plus those of Dichomitus squalens, Fomitopsis GLX belongs to the superfamily of copper-radical oxi- pinicola, Gelatoporia subvermispora (syn.: Ceriporiopsis subvermis- dases (Whittaker et al. 1996) whose distribution in Poly- pora), P. chrysosporium, R. placenta, Trametes versicolor and Wol- porales genomes has been reported (Kersten and fiporia cocos (5 Wolfiporia extensa) available at the JGI Cullen 2014). In contrast all other oxidases mentioned MycoCosm portal (http://genome.jgi.doe.gov/programs/ above are flavooxidases from the GMC oxidoreductase fungi) (Grigoriev et al. 2012) were screened for genes of superfamily whose first three members were GOX, the AAO, MOX, GOX, CDH, P2O and PDH families in the GMC superfamily. Among the above genomes, those from MOX and choline dehydrogenase (Cavener 1992). the Antrodia clade (F. pinicola, R. placenta, W. cocos) corre- Two additional GMC enzymes, which are inefficient spond to wood decay by brown-rot species while the other reducing O2 to H2O2, are cellobiose dehydrogenase species (B. adusta, D. squalens, Ganoderma sp., G. subvermispora, (CDH, EC 1.1.99.18) and pyranose dehydrogenase P. chrysosporium, P. brevispora and T. versicolor) cause white-rot (PDH, EC 1.1.99.29) (Zámocký et al. 2006, Kruså et al. decay of wood. 2008, Peterbauer and Volc 2010). All members of the The screening for each of the GMC families was performed GMC superfamily share similar structural features by querying an entire set of filtered model proteins for each (Wierenga et al. 1986, Kiess et al. 1998). Recently several of the genomes with the following (GenBank) reference GMCs have been classified in the so-called subfamilies sequences: (i) AAO from Pleurotus eryngii (AAC72747); (ii) AA3_1 (CDH), AA3_2 (AAO/GOX), AA3_3 (MOX) MOX from Gloeophyllum trabeum, Pichia methanolica and Candida boidinii (ABI14440, AF141329 and Q00922); (iii) GOXs from and AA3_4 (P2O) of the CAZy database (Levasseur et al. ‐ 2013), but this nomenclature is not used here. Talaromyces flavus, Penicillium expasum, Penicillium amagasa kiense, Aspergillus niger and Botryotinia fuckeliana (AAB09442, Three representative Polyporales—Bjerkandera adu- ABN79922, AAD01493, AAF59929 and CAD88590); (iv) CDHs sta, Ganoderma sp. (G. lucidum complex) and Phlebia bre- — from P. chrysosporium, G. subvermispora, Coniophora puteana, Pycno- vispora were sequenced (Hibbett et al. 2013) and porus cinnabarinus (syn.: Trametes cinnabarina)andT. versicolor their different GMC gene families are analyzed here. (CAA61359, ACF60617.1, BAD32781 AAC32197, AAC50004); Bjerkandera adusta is a strong lignin degrader, which (v) P2Os from T. versicolor, Peniophora sp., P. chrysosporium, Lyo- produces AAO (Muheim et al. 1990) together with lig- phyllum shimeji and G. trabeum (BAA11119, AAO13382, ninolytic peroxidases (Kimura et al. 1991, Heinfling AAS93628, BAD12079 and ACJ54278); (vi) PDHs from Leucoa- et al. 1998). Some species of Ganoderma cause extensive garicus meleagris (syn.: Agaricus meleagris), Agaricus xanthodermus wood delignification (González et al. 1986; Martínez and Agaricus bisporus (AAW82997, AAW92123 and AAW92124). et al. 1995, 2011) and little is known about GMC pro- — duction by these fungi (Peláez et al. 1995, Ralph et al. Sequence analysis. The genomic sequences with the highest 1996). Finally, P. brevispora was investigated for wood similarities with the reference sequences for the different GMC families first were examined for the automatically biopulping due to selective lignin removal (Akhtar annotated introns, searching for consensus 59–39 and lariat et al. 1993, Fonseca et al. 2014). Moreover, seven addi- sequences (Ballance 1986), as well as for the annotation tional sequenced Polyporales genomes were screened of N- and C-termini. The presence/absence of secretion sig- and included in the present comparative analysis of nal peptides predicted by the JGI automatic annotation pipe- GMC-encoding genes. The present study is part of a line was manually revised to detect possible mistakes (e.g. wider genomic project covering other gene families in neighbor introns) that could result in inaccurate FERREIRA ET AL.: GMC GENES IN POLYPORALES GENOMES 1107

TABLE I. Inventory of 95 genes from six GMC families in the genomes of 10 Polyporales species (BJEAD, B. adusta; PHLBR, P. brevispora; PHACH, P. chrysosporium; DICSQ, D. squalens; GANSP, Ganoderma sp., TRAVE, T. versicolor; GELSU, G. subvermispora; FOMPI, F. pinicola; RHOPL, R. placenta; and WOLCO, W. cocos) from four clades, producing white-rot and brown-rot decay of wood

Phlebioid Core polyporoid Gelato-poria Antrodia

Clade BJEAD PHLBR PHACH DICSQ GANSP TRAVE GELSU FOMPI RHOPL WOLCO AAO 11 3 3 8 7 3 4 1 2 0 MOX 5 6 3 4 4 4 1 4 4 4 GOX 0 0 1 0 0 0 0 0 2 0 CDH 1 1 1 1 1 1 1 0 0 0 P2O 1 1 1 0 0 1 0 0 0 0 PDH 0 0 0 0 0 0 0 0 0 0 All GMCs 18 11 9 13 12 9 6 5 8 4 Ecology White rot Brown rot

Four allelic variants (SUPPLEMENTARY TABLE I) are excluded from the inventory. predictions, followed by inspection of the eventually revised Reconciliation analyses.—The histories of gene duplication and sequences with the Signal P 4.0 server (www.cbs.dtu.dk/ losses for total GMCs (and the individual families) were services/SignalP-4.0) (Petersen et al. 2011). Moreover, other inferred with Notung 2.6 (Durand et al. 2006). The gene servers as TargetP 1.1 (Emanuelsson et al. 2000), WoLF tree was used as input and combined with a Polyporales phy- PSORT (Horton et al. 2007) and TMHMM 2.0 were used to logenetic tree (Binder et al. 2013) from TreeBASE (www. confirm the secreted nature of proteins as well as to predict treebase.org, tree ID Tr67497). The estimated numbers of their putative subcellular locations. Predictions were con- gene duplications and deletions on each branch were used firmed by multiple alignment with MUSCLE (Edgar 2004) to hypothesize the number of sequences at the ancestral and by the comparison with reference sequences. Multiple nodes. Two different threshold levels (30% and 90%) were alignments also were used for analysis of motifs conserved used to assess the significance of the predictions obtained. in GMC proteins (the ADP-binding domain and, at least, one of the two characteristic Prosite PS00623 and PS00624 RESULTS sequences) (Cavener 1992). The sequences that lacked these GMC conserved motifs were discarded. GMC gene families in three recently sequenced and other Poly- Finally, molecular models of 94 out of the 95 GMC porales genomes.—A total of 41 GMC genes—21 AAO, 15 sequences (references in SUPPLEMENTARY TABLE I) could be MOX, 3 CDH and 2 P2O genes (TABLE I)—were iden- generated at the Swiss-Model server (www.swissmodel. tified in the recently sequenced genomes of B. adusta, expasy.org), which selected the most adequate templates Ganoderma sp. and P. brevispora. Family classification (Bordoli et al. 2009). For AAO, MOX, GOX, CDH and P2O was completed by inspection of the enzyme molecular sequences, the crystallographic structures of P. eryngii AAO models described below for characteristic flavin envir- (PDB 3FIM), Arthrobacter globiformis choline oxidase (PDB onment and catalytic residues (Gadda 2008, Hernán- 3LJP, note that no MOX crystal structure is available), A. niger GOX (PDB 1CF3), P. chrysosporium CDH (PDB dez-Ortega et al. 2012a, Wongnate and Chaiyen 2013, 1KDG) and Aspergillus oryzae P2O (PDB 1TTO), respectively, Romero and Gadda 2014). The genome of B. adusta were used as templates. Strictly conserved histidine and histi- has the highest number of GMC genes (a total of dine/asparagine residues at the active site (Hernández- 18), while similar numbers (11–12 genes) were found Ortega et al. 2012c, Wongnate et al. 2014) were searched in the two other genomes (TABLE I). No GOX or for in all the models, and sequences lacking these residues PDH genes were found in any case and P2O genes were discarded. also were absent from the Ganoderma sp. genome. AAO genes are the most abundant GMC genes in — GMC evolutionary history. The evolutionary history of the B. adusta and Ganoderma sp. (11 and 7, respectively) (95) GMC sequences obtained was estimated with RaxML while MOX genes are the most abundant in P. brevis- 7.7.1 (Stamatakis et al. 2008) from the multiple alignment pora (six genes). None of the 41 GMC genes identified obtained with MEGA 5 (Tamura et al. 2011) (alignment in in the three genomes had been cloned and deposited SUPPLEMENTARY FIG. 1). For evolutionary tree construction, a maximal likelihood with clustering method was used, in databases (TABLE I). with the WAG model of amino acid substitutions, and the Annotated genomes from seven more species of gaps treated as deletions (a 100-iteration bootstrap was per- Polyporales were included for a wider comparison. formed). Identity degrees among all the above sequences The resulting 10 genomes include representatives of were obtained after pairwise alignment with Clustal W2. the Phlebioid (B. adusta, P. brevispora, P. chrysosporium), 1108 MYCOLOGIA

FIG. 1. Ribbon models for the molecular structures of representative members of the five GMC oxidoreductase families found in 10 Polyporales genomes (flavin and heme cofactors are shown as sticks). A. AAO of B. adusta (JGI protein ID 245059) indicating the position of four b-sheets, individual b-strands and 19 a-helices. B. MOX (monomer) of F. pinicola (JGI protein ID 156775). C. GOX (monomer) of P. chrysosporium (JGI protein ID 131961). D. CDH of G. subvermispora (JGI protein ID 84792) (flavin domain in the left and heme domain in the right. E. P2O (monomer) of B. adusta (JGI protein ID 34622). The molecular models were built with crystal structures of related proteins as templates. core Polyporoid (D. squalens, Ganoderma sp., T. versico- more abundant in the genomes of white-rot (av. 5.7 lor) Gelatoporia (G. subvermispora) and Antrodia (F. pini- genes/genome) than brown-rot (av. 1.0 gene/genome) cola, R. placenta, W. cocos) clades (Binder et al. 2005). species. Moreover, CDH genes were present in all the The number of genes of the different GMC families in white-rot genomes (one copy per genome) but absent each of the genomes is included herein (TABLE I), up from the brown-rot genomes. Finally, P2O genes also to a total of 95 (JGI protein ID references are included were absent from the brown-rot genomes and no PDH [SUPPLEMENTARY TABLE I], as well as the existence of genes were found in any of the genomes. alleles and recognized signal peptides; and the complete sequences are provided in the alignment [SUPPLE- Structural modeling of GMC oxidoreductases from Polyporales MENTARY FIG. 1]). MOX genes are equally present in the genomes.—Most of the predicted GMC sequences (94 white-rot and brown-rot genomes (average 4.0–4.4 of 95) were modeled with related crystal structures genes/genome) while those of AAOs are nearly sixfold as templates. Five representative structures (FIG.1) FERREIRA ET AL.: GMC GENES IN POLYPORALES GENOMES 1109

FIG. 2. Detail of active-site residues in the molecular models for the ﬁve Polyporales GMCs (FIG. 1). A. B. adusta AAO. B. F. pinicola MOX. C) P. chrysosporium GOX. D. G. subvermispora CDH. E. B. adusta P2O. Residue numbering corresponds to the putative mature proteins. FAD and the selected residues are shown as sticks. The N390-T402 loop of AAO is shown in A. correspond to B. adusta AAO (A), F. pinicola MOX (FIG 2A), F. pinicola MOX His535 (FIG.2B),P. chrysos- (B), R. placenta GOX (C), G. subvermispora CDH (D) porium GOX His538 (FIG.2C),G. subvermispora CDH and B. adusta P2O (E) mature proteins. All these His688 (FIG. 2D) and B. adusta P2O His540 (FIG. GMCs show a common folding with the lower 2E). A second conserved histidine in AAO and domain harboring the FAD cofactor. Specific fea- GOX (His541 and His581 in FIG. 2A, C, respectively) tures are present in AAO, which possesses a loop par- is replaced by an asparagine in MOX, CDH and P2O tially covering the entrance to the active site (FIG.2A, proteins (Asn 578, Asn731, Asn583; FIG.2B,D,E, left); and CDH, which has a heme domain connected respectively). An aromatic residue often precedes by an unstructured linker (FIG. 1D). Of interest, the fully conserved histidine, being a tryptophan in AAOs and CDHs are known as monomeric proteins AAO (Trp496) and MOX (Trp534) and a phenylala- while GOXs, P2Os and MOXs form oligomers nine in GOX (Phe537), while a leucine (Leu539) (Romero and Gadda 2014). One large b-sheet is pre- and an asparagine (Asn687) occupy this position in sent in both the FAD-binding (sheet A) and the sub- the P2Os and CDHs, respectively (FIG.2).Atthe strate-binding (sheet C) domains, the former being opposite (si) side of the isoalloxazine ring another accompanied by two small sheets (B, D) and the lat- aromatic residue, which points toward the active ter by only one (sheet E) (FIG.1A).Similarnumbers site, is conserved, being a phenylalanine in AAO of a-helices exist in the FAD-binding and the sub- (Phe90) and a tyrosine in MOX (Tyr99) and GOX strate-binding domains (9–10 in AAO), some (Tyr90) (FIG. 2A–C). An asparagine preceding the ofthem(e.g.AAOhelices1,4,10)conservedin latter position is conserved in all the Polyporales most GMCs. All the predicted models present the GMCs (Asn89, Asn98, Asn89, Asn322 in FIG.2AAO, ADP-binding bab motif near their N-termini (SUP- GOX, MOX, CDH, respectively) with the exception PLEMENTARY FIG. 2A) and the GMC signatures 1 of P2Os. This asparagine residue, also conserved in and 2 (Prosite PS00623 and PS00624, respectively; other GMCs, is involved in flavin bent conformation SUPPLEMENTARY FIG.2B,C),withtheonlyexception (Kiess et al. 1998). of P2O that lacks signature 1. The FAD flavin ring enters the GMC upper Evolutionary history of GMC oxidoreductases in the Polypor- domain, where several residues form a substrate- ales genomes.—The evolutionary history of the 95 binding site at the re-side of the isoalloxazine ring GMCs identified in the 10 Polyporales genomes (FIG. 2). They include a histidine strictly conserved (five allelic variants, SUPPLEMENTARY TABLE I, in the superfamily (SUPPLEMENTARY FIG. 1multiple excluded) was inferred by comparing their predicted alignment), corresponding to B. adusta AAO His497 amino-acid sequences (mature proteins). It is worth 1110 MYCOLOGIA

B. adusta AAOs are included in a 13-member subgroup (a, 100% bootstrap), suggesting recent duplication. In contrast MOXs include a subgroup (b, 100% bootstrap) of 10 sequences, each from one of the genomes. These 10 sequences share an insertion and a slightly longer C-terminus (SUPPLEMENTARY FIG. 1) involved in oligomerization and/or secretion of the enzymes through a unique secretory pathway (Danneel et al. 1994), suggesting a common origin of these genes. At the basal nodes the well supported (100% bootstrap) P2O (four sequences) and CDH (seven sequences) families appear unrelated between them and with the rest of the GMCs. The distant position of the latter families and the related- ness between AAOs, GOXs and MOXs agree with the pairwise identity values across and within gene families (SUPPLEMENTARY FIG. 3). In fact the average pairwise (interfamily) identity between P2O and CDH sequences is 8% and, among them and the rest of the families, range between 11% and 14%. These values are significantly lower than those between AAO and MOX (25% interfamily average), GOX and MOX (24% interfamily average) and AAO and GOX sequences (31% interfamily average). On the other hand the pairwise (intrafamily) identities within the CDH and P2O families are higher, 73% and 51%, respectively; whereas AAOs, GOXs and MOXs show values of 46%, 30% and 57%, respectively.

GMC gene duplication and loss during diversification of Polyporales.—The expansion or reduction in the number of GMC genes upon evolution of Polyporales was investigated by reconciliation of the evolutionary tree of the 95 GMC genes (FIG. 3) and the phylogenetic tree of the 10 species of Polyporales (from TreeBASE) using Notung. The results (using two different threshold levels) suggest that the ancestors of Polyporales FIG. 3. Maximal likelihood evolutionary tree of the 95 had a high number of GMC genes, more than found GMC sequences (ﬁve allelic variants listed in SUPPLEMENTARY in any of the extant species or the predicted intermedi- TABLE I excluded) from 10 Polyporales genomes (different color labels), prepared with RaxML (with gaps treated as ate ancestors (FIG. 4). Therefore during GMC evolu- deletions). The AAO, MOX, P2O and CDH groups (and the tion 14 contraction events and two expansions (from a and b subgroups mentioned in the text) are shown, nodes d to node g and from node e to node h) were together with a few GOX sequences related to AAOs. predicted. A similar tendency was observed for each Numbers at nodes indicate bootstrap values. Those modeled of the individual GMC families (SUPPLEMENTARY FIG. sequences (FIGS. 1, 2) are indicated by arrows. Abbreviations 4A–E) with a total of 39 contractions and seven expan- of the fungal species are provided (TABLE I) as are complete sions. In this case expansions resulted in higher AAO amino-acid sequences (SUPPLEMENTARY FIG. 1). (in node g and in B. adusta;SUPPLEMENTARY FIG. 4A), GOX (in R. placenta;SUPPLEMENTARY FIG. 4C) and noting that all the sequences from each of the GMC P2O (in node c;SUPPLEMENTARY FIG. 4E) gene num- families cluster together in the maximal likelihood bers (often after previous contractions) than predicted tree (FIG. 3). The two main groups correspond to for the initial Polyporales ancestor. The stronger con- the 39 MOXs and the 42 AAOs (100% and 79% boot- traction of GMC gene numbers was evident in the strap, respectively), with the only 3 GOXs distantly Antrodia clade, resulting in only 4–5 genes in W. cocos associated to the AAOs. Of interest, 10 of the 11 and F. pinicola, and the largest expansion was observed FERREIRA ET AL.: GMC GENES IN POLYPORALES GENOMES 1111

generates H2O2 by redox-cycling of anisaldehyde (Guillén and Evans 1994), an extracellular fungal metabolite (Gutiérrez et al. 1994). Subsequent studies focused on the Pleurotus eryngii enzyme, which was cloned and sequenced (Varela et al. 1999), heterolo- gously expressed (Varela et al. 2001, Ruiz-Dueñas et al. 2006), crystallized (Fernández et al. 2009) and its reaction mechanisms investigated by a variety of techni- ques (Ferreira et al. 2005, 2006, 2009, 2010, 2015; Hernández-Ortega et al. 2011a, b, 2012b, c). Then a Polyporales AAO was isolated from B. adusta (Muheim et al. 1990). Although the above enzymes are known as secreted proteins (Hernández-Ortega et al. 2012a), recognized signal peptides are missing from four of the 42 sequences from the 10 Polyporales genomes, including one sequence from B. adusta and two from D. squalens and P. chrysosporium. The latter is in agreement with the description of an intracellular AAO in this fungus (Asada et al. 1995). AAO activity has been detected in cultures of a few other Polyporales species (Peláez et al. 1995), although FIG. 4. Estimated range of GMC gene copies at the ancestral nodes (and extant species) of the represented a Southern blot (using a P. eryngii probe) did not detect phylogeny of Polyporales taken from Binder et al. (2013) the corresponding gene in many of these (Varela et al. after reconciliation with the gene evolutionary history (FIG. 2000), suggesting gene variability among different 3) using Notung (Durand et al. 2006). Branches and fungi. AAO activity in B. adusta (Romero et al. 2010), numbers after gene expansion and contraction are in black whose sequence corresponds to BJEAD_171002 from and gray, respectively (for reconciliation of the individual the JGI genome, has been characterized largely show- GMC families, see SUPPLEMENTARY FIG. 4). ing higher activity on p-hydroxy and chlorinated benzyl alcohols than Pleurotus AAO (Romero et al. 2009). p-Hydroxybenzyl alcohols are the typical substrates of in B. adusta (Phlebioid clade) with 18 GMC genes, vanillyl alcohol oxidase, a flavoenzyme from a different including 11 AAOs (FIG. 4). Of interest, most of the superfamily (Leferink et al. 2008), but they are not effi- remaining GMC genes in the Antrodia clade corre- ciently oxidized by Pleurotus AAO, whose best sub- spond to the MOX family (4/5 in F. pinicola, 4/8 in strates are p-methoxylated benzyl alcohols (Guillén R. placenta and 4/4 in W. cocos). et al. 1992, Ferreira et al. 2005). Therefore the best characterized Polyporales AAO shows catalytic properties intermediate between Agaricales AAO and vanillyl- DISCUSSION alcohol oxidase. The higher activity of B. adusta AAO The global reaction in initial wood decay by white-rot on chlorinated benzyl alcohols, which was noticed first and brown-rot basidiomycetes is iron-catalyzed oxida- by de Jong et al. (1994), is related to the ability of this tion of lignin or polysaccharides, respectively, by species to synthesize 3-chloro-p-methoxybenzaldehyde H2O2 generated by oxidases (from the GMC and/or (de Jong et al. 1992, de Jong and Field 1997). Redox the copper-protein radical superfamilies). In white- cycling of this and related chlorinated compounds pro- 3+ rot decay this reaction is catalyzed by Fe in the vides a continuous source of H2O2 to B. adusta peroxi- heme cofactor of ligninolytic peroxidases, while in dases (de Jong et al. 1994), similar to the Pleurotus 2+ brown-rot decay free Fe reduces H2O2 forming the anisaldehyde redox cycling. Chloroaromatics also highly reactive hydroxyl radical (Martínez et al. 2005; could help wood colonization due to their antibiotic Kersten and Cullen 2007; Baldrian and Valaskova properties. 2008, 2009). The information available on the presence and relevance of GMC families in Polyporales Glucose oxidase.—In contrast to AAO, which has been species is discussed below. reported rarely in ascomycetes (Goetghebeur et al. 1992), GOX has been largely studied in A. niger (Fre- Aryl-alcohol oxidase.—AAO first was isolated from Pleuro- derick et al. 1990) and other ascomycetous fungi but tus species (Agaricales) (Bourbonnais and Paice 1988; rarely in basidiomycetes (Danneel et al. 1993). This is Guillén et al. 1990; Sannia et al. 1991, 1992) where it the protein with the largest sequence identity with 1112 MYCOLOGIA

AAO, as shown in the gene tree, both sharing the gen- been demonstrated and operation of an alternative eral folding and active-site residues (Hecht et al. 1993, secretion mechanism was proposed (Daniel et al. Wohlfahrt et al. 1999, Witt et al. 2000). 2007). The rationale for MOX involvement in brown- GOX is widely used in biosensors and other biotech- rot decay is that demethoxylation, resulting in metha- nological applications (Bankar et al. 2009), but its nol release, was reported first by Kirk (1975) and con- involvement in lignocellulose degradation was dis- firmed by 2D-NMR analyses (Martínez et al. 2011) as carded because the best known representatives are the main lignin modification in brown-rot decay. confirmed intracellular enzymes. However, two of the only three GOX sequences identified in the Polypor- Pyranose and cellobiose dehydrogenases.—PDH and CDH ales genomes include a typical signal peptide, suggest- use electron acceptors different from O2 and therefore ing participation in the extracellular attack on do not contribute to H2O2 supply. However, they oxidize lignocellulose. plant carbohydrates and participate in electron transfer to other lignocellulose-degrading oxidoreductases. Pyranose 2-oxidase.—P2O, which differs from GOX in PDH catalyzes the same oxidations of P2O but uses glucose oxidation at the C2 (instead of the C1) posi- quinones as electron acceptors, being an enzyme of tion, is known as a secreted enzyme (Daniel et al. interest in biotechnology (Peterbauer and Volc 1994) involved in lignocellulose degradation (Nyan- 2010). The first PDH was isolated from Agaricus bis- hongo et al. 2007). This oxidoreductase first was inves- porus (Volc et al. 1997) and also found in related spe- tigated in P. chrysosporium (Artolozaga et al. 1997), and cies (Kujawa et al. 2007, Kittl et al. 2008) including these studies suggested that P2O rather than GOX is L. meleagris where it was thoroughly investigated (Tan secreted during wood decay (Volc et al. 1996). How- et al. 2013; Krondorfer et al. 2014a, b). Screening for ever, none of the four genes found in the Polyporales PDH revealed its exclusive presence in the above and genomes have a recognized signal peptide, in agree- other litter-degrading Agaricales (Volc et al. 2001), ment with the sequence obtained by Koker et al. an observation that is consistent with its absence from (2004) for the cloned P2O gene from P. chrysosporium. all the (wood-rotting) Polyporales genomes analyzed. Therefore if secreted this would be by an alternative CDH includes both flavin and heme domains, the mechanism, as suggested for MOX (see below). former being able to oxidize cellobiose to cellobiolac- + P2O is produced by other Polyporales, including tone by transferring the electrons to Fe3 via the Trametes multicolor (5 Trametes ochracea) (Leitner et al. heme domain (Henriksson et al. 2000, Zámocký et al. 2001), and most recent P2O research focuses on this 2006). CDH first was described in P. chrysosporium enzyme, whose reaction mechanisms have been eluci- (whose conidial state was referred as Sporotrichum pul- dated in a variety of crystallographic, spectroscopic, verulentum in some of these studies) (Ayers et al. directed mutagenesis, isotope labeling and kinetic stu- 1978, Bao et al. 1993). The ancestral fusion between dies (Hallberg et al. 2004; Sucharitakul et al. 2008; the two CDH domains and the subsequent evolution Prongjit et al. 2009, 2010; Pitsawong et al. 2010; Wong- in different fungi has been discussed (Zámocký et al. nate et al. 2011, 2014). 2004). One CDH gene was present in the genomes of the seven white-rot Polyporales analyzed and absent Methanol oxidase.—MOX is mostly known as a peroxyso- from the three brown-rot Polyporales genomes, in mal enzyme in methylotrophic ascomycetous yeasts, agreement with Hori et al. (2013), in which CDH was such as Pichia pastoris or C. boidinii (Ozimek et al. found only in white-rot genomes. However, this GMC 2005). The first basidiomycete MOX was purified and seems to be present in other brown-rot fungi, as characterized from P. chrysosporium (Nishida and Eriks- revealed by its early description in C. puteana (order son 1987) and it is also known from Phlebiopsis gigantea Boletales) (Schmidhalter and Canevascini 1993) and (Danneel et al. 1994). MOX was proposed as the main its detection in the genomes of brown-rot fungi from oxidase in brown-rot decay based on biochemical char- other Agaricomycotina orders (Floudas et al. 2012). acterization and expression analyses in Gloeophyllum Its ability to generate hydroxyl radical by simulta- 3+ trabeum (Daniel et al. 2007). The corresponding gene neous Fe and O2 reduction has been suggested (Kre- is present in the genome of R. placenta (Martinez et al. mer and Wood 1992), but O2 reduction by CDH is + 2009) and was overexpressed in wood-containing cul- inefficient and only takes place in the absence of Fe3 . tures of this brown-rot fungus and also in those of the However, recent studies showed that CDH increases white-rot P. chrysosporium (Vanden Wymelenberg et al. the cellulolysis yield and contributes to the action 2010). of lytic polysaccharide monooxygenase (Langston et al. The MOX gene of G. trabeum and other basidiomy- 2011). cetes does not include a recognized signal peptide. CDH from P. chrysosporium experiences proteolytic However, the extracellular location of MOX has cleavage in cultures releasing the flavin domain (Wood FERREIRA ET AL.: GMC GENES IN POLYPORALES GENOMES 1113 and Wood 1992), which was described as a different Polyporales included loss of the ligninolytic peroxi‐ enzyme, cellobiose-quinone oxidoreductase (Wester- dase genes, which are not required because lignin mark and Eriksson 1974). However, the physiological remained polymeric in brown-rotted wood. However, significance of such cleavage and the role of cellobiose- extracellular H2O2, used as peroxidase-activating sub- quinone oxidoreductase under natural conditions is strate in white-rot decay, also plays an important role unknown (Raices et al. 2002). in brown-rot decay as the precursor of the hydroxyl radical formed by Fenton reaction. Therefore it seems GMC oxidoreductases in Polyporales: final evolutionary/eco- that the same H O -generating oxidase types present — 2 2 logical remarks. The total number of GMC genes in white-rot fungi remained in the derived brown-rot cloned to date from species of the order Polyporales species. During evolution some differences in the fre- is fewer than 10: from P. chrysosporium, P. cinnabarinus, quency of the individual GMC families appeared. In Pycnoporus sanguineus (syn.: Trametes sanguinea), T. this way MOX genes are the most abundant GMC ochracea and T. versicolor (Leitner et al. 1998, Raices genes in the brown-rot Polyporales while AAO genes et al. 1995, Dumonceaux et al. 1998, Moukha et al. are the most abundant in the white-rot species (up to 1999, Vecerek et al. 2004, de Koker et al. 2004, Sulej 11 copies in B. adusta). Finally, the number of CDH et al. 2013). However, the present survey of GMC genes predicted in the ancestor of Polyporales dimin- genes from a broader sampling including 10 Polypor- ished, but all the white-rot species maintain one CDH ales genomes (from different clades and survival strate- gies) reveals nearly 100 GMC genes representing five gene, which contributes to polysaccharide degradation of the six best-known families (no PDH genes present). by these fungi. However, CDH genes disappeared in The GMC superfamily is thought to have evolved brown-rot fungi, where Fenton chemistry is the main from an old common ancestor, which very likely exhib- mechanism for polysaccharide attack. ited broad substrate specificity and poor kinetic para- meters and gave rise to more specialized and efficient ACKNOWLEDGMENTS enzymes as evolution proceeded (Cavener 1992). The present study suggests that this diversification took This work was supported by the INDOX (www.indoxproject. place at a more ancestral stage of fungal evolution, eu; KBBE-2013-7-613549) European project and by the Span- ish HIPOP (BIO2011-26694) project. The work conducted by with predominant gene loss among members of the the U.S. Department of Energy Joint Genome Institute was Polyporales. This resulted in two main GMC types supported by the Office of Science of the U.S. Department (groups) corresponding to AAO and MOX, with an , of Energy under Contract DE-AC02-05CH11231, in the average of 4 gene copies per genome, and three frame of the JGI Saprotrophic Agaricomycotina project coor- small groups corresponding to P2O, CDH and GOX dinated by D.S. Hibbett (Clark University, USA). J.M. Barrasa – (neighbor to the AAO group) with 0 1 copies per gen- (University of Alcalá, Spain) is acknowledged for useful com- ome, in agreement with Zámocký et al. 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TABLE SI. JGI (www.jgi.doe.gov) references (protein ID #) for the 95 GMC genes (plus 5 alleles) identified in the genomes of ten wood-rotting Polyporales (for species abbreviations see TABLE I; the existence of alleles and recognized signal peptides is indicated, see notes below) ------White-rot ------Brown-rot ------BJEAD PHLBR PHACH DICSQ GANSP TRAVE GELSU FOMPI RHOPL WOLCO 52991# 22550 6199 96414 67648 40237 84544 40728 44654* 66377 131358 37188 102587 67654 176148 117387 54008 71431 164178 135972 103879 85135 133945 118493 55496 114902 86071 117498 137959 58266*

114954 160139 124428 156054 160546 130042 AAO 171002 171752 138009 171059 182736 183896 245049 245297 34705 27956 5574 149587 114505 144610 80773 90445 55972 24953 45314 29466 6010 157363 116436 167157 127556 56055* 25722

143000 128210 126879 173648 116439 170473 129478 106935 121505 227734 128980 181599 130292 43286 156775 118723 132654 MOX 241975 157963 126217* 157964 129158 129841* 131961 108849

GOX 128830

45135 160653 11098 153749 86428 73596 84792 CDH

34622 123747 137275 174721 P2O

* RHOPL_129841, RHOPL_126217 and RHOPL_56055 are allelic variants of MOX RHOPL_118723, RHOPL_129158 and RHOPL_55972, respectively; while RHOPL_44654 and RHOPL_58266 were considered as variants of AAO RHOPL_55496. # The protein models including a recognized signal peptide are underlined.

1 FIG. S1. Alignment of the complete 95 GMC sequences. Numbers correspond to amino

2 acidic sequences in mature proteins (signal peptides numbered using negative values).

3 The variable conservation of residues equivalent to AAO/GOX/MOX/P2O/CDH

4 N89/N89/N98/S128/N302 (highlighted in blue), F90/Y90/F99/F131/G303 (in red),

5 W496/F537/W534/L539/N687 and H497/H538/H535/H540/H688 (in yellow), and

6 H541/H581/N578/N583/N731 (in green), located near the flavin ring (see FIG. 2), is

7 indicated. The insertions and C-termini of MOX sequences belonging to subcluster b

8 (see FIG. 3) is highlighted in purple.

10 FIG. S2. Sequence logo of the ADP-binding motif (A), with consensus sequence [DP]-x-

11 [VIL]-[VI]-x-G-x-G-x(2)-[GA]-x(3)-A-X-[RKT]-L-x(7)-[VT]-x(2)-[LIV]-E-x-G, and

12 GMC signatures 1 (B) and 2 (C), with consensus sequences [GA]-[RKNC]-x-[LIVW]-

13 G(2)-[GST](2)-x-[LIVM]-[NH]-x(3)-[FYWA]-x(2)-[PAG]-x(5)-[DNESHQA] and

14 [GS]-[PSTA]-x(2)-[ST]-[PS]-x-[LIVM](2)-x(2)-S-G-[LIVM]-G respectively, in 95

15 GMC sequences (TABLE SI) from 10 Polyporales genomes. The overall height of each

16 stack represents the sequence conservation at that position, and the height of each letter

17 reflects the relative frequency of the corresponding amino acid. Residues in A, B and C

18 correspond to positions 2-33, 73-100 and 264-283, respectively in B. adusta AAO (JGI

19 protein ID 245059), and equivalent positions in the other GMCs.

21 FIG. S3. Pairwise identity matrix among the 95 GMC sequences included in Fig. S1.

22 Numbers indicate percentage of identities and colors range from green (most similar) to

23 red (lest similar).

24 2

25 FIG. S4. Estimated range of individual AAO (A), MOX (B), GOX (C), CDH (D) and

26 P2O (E) gene copies, and total GMC from sum of family gene numbers (F), at the

27 ancestral nodes (and extant species) of the represented phylogeny of Polyporales taken

28 from Binder et al. (2013) after reconciliation with the gene phylogeny (FIG. 2) using

29 Notung (Durand et al. 2006). Branches and numbers after gene expansion and

30 contraction are in blue and red color, respectively. See Fig. 4 for gene reconciliation in

31 the whole GMC superfamily. TRAVE_174721 0 ------0 PHLBR_123747 0 ------0 PHACH_137275 0 ------0 BJEAD_34622 0 ------0 GELSU_84792 -20 -MFGRFLLALLPLVGSVLSQSGSSYTDPDNGFVFNGITDPVYGVTYGVVFPEPSSSGTYPDEFIGEIVAPLTAEWIGVSFGGAMLDCLLLVAWPNEDSIVASTRYATDYVQPTEYDGP-V 98 TRAVE_73596 -21 MKFKSLLLSLLPLVGSVYSQVAAPYVDSGNGFVFDGVTDPVHSVTYGIVLPQAST----STEFIGEFVAPNEAQWIGLALGGAMIGNLLLVAWPDGNKIVSSPRYATGYTLPAAYAGP-T 94 DICSQ_153749 -21 MKSKRLLFSLLPFVGTAFAQVAAPYTDSGNGFVFDGITDPTYGVTYGIVLPQANT----STEFIGEIVAPIAAKWVGVAFGGAMIGDLLLVAWPNGNDIVASTRWATDYIQPTEYDGP-T 94 GANSP_86428 -21 MKLKRSLLSLLPIVGSALAQVAAPYTDPGNGFVFDGITDAVYGVQYGIVLPQADS----STEFIGEIVAPIAAKWIGWAFGGAMIGDLLLVAWPNGNDIVASTRYATAYAQPTAYDGP-T 94 PHLBR_160653 -20 -MLRRSLLTLLPFIGTALSQSASTFVDPVNGYQFTGLTDPVHDVTYGFTFPPLPTSGSDSTEFIGEIVAPIDSQWIGLALGGDMIQDLLLVAWPNDGDIVFSTRWATDYIQPVAYTGDAT 99 BJEAD_45135 -20 -MLRRSLFALLPLVGTAFSQLASQFTDPGNGFQFTGLTDPVHSVTYGFVFPPLATSGAQSTEFIGEIVAPLAAKWVGVALGGAMNGDLLLMAWPNGNDIVFSTRFSTSYALPPPYTGDAV 99 PHACH_11098 -20 -MLGRSLLALLPFVGLAFSQSASQFTDPTTGFQFTGITDPVHDVTYGFVFPPLATSGAQSTEFIGEVVAPIASKWIGIALGGAMNNDLLLVAWANGNQIVSSTRWATGYVQPTAYTGTAT 99 GANSP_114505 0 ------0 DICSQ_181599 0 ------0 TRAVE_144610 0 ------0 PHLBR_128980 0 ------0 BJEAD_34705 0 ------0 PHACH_126879 0 ------0 FOMPI_127556 0 ------0 GELSU_80773 0 ------0 RHOPL_118723 0 ------0 WOLCO_24953 0 ------0 TRAVE_43286 0 ------0 DICSQ_149587 0 ------0 GANSP_116439 0 ------0 DICSQ_173648 0 ------0 GANSP_116436 0 ------0 PHLBR_157963 0 ------0 PHLBR_157964 0 ------0 PHLBR_128210 0 ------0 BJEAD_143000 0 ------0 BJEAD_45314 0 ------0 PHACH_5574 0 ------0 BJEAD_241975 0 ------0 PHLBR_29466 0 ------0 RHOPL_129158 0 ------0 RHOPL_55972 0 ------0 BJEAD_227734 0 ------0 PHLBR_27956 0 ------0 PHACH_6010 0 ------0 DICSQ_157363 0 ------0 GANSP_130292 0 ------0 TRAVE_167157 0 ------0 TRAVE_170473 0 ------0 RHOPL_106935 0 ------0 WOLCO_121505 0 ------0 WOLCO_132654 0 ------0 FOMPI_90445 0 ------0 WOLCO_25722 0 ------0 FOMPI_129478 0 ------0 FOMPI_156775 0 ------0 DICSQ_160139 0 ------0 GANSP_124428 0 ------0 DICSQ_171752 0 ------0 GANSP_67648 0 ------0 DICSQ_102587 0 ------0 GANSP_67654 0 ------0 GANSP_85135 0 ------0 DICSQ_160546 0 ------0 PHLBR_22550 0 ------0 TRAVE_176148 0 ------0 GANSP_117498 0 ------0 DICSQ_182736 0 ------0 TRAVE_40237 0 ------0 TRAVE_133945 0 ------0 GANSP_130042 0 ------0 GANSP_138009 0 ------0 DICSQ_103879 0 ------0 DICSQ_96414 0 ------0 FOMPI_40728 0 ------0 RHOPL_55496 0 ------0 GELSU_118493 0 ------0 RHOPL_54008 0 ------0 GELSU_137959 0 ------0 GELSU_117387 0 ------0 GELSU_84544 0 ------0 PHACH_37188 0 ------0 PHACH_135972 0 ------0 DICSQ_86071 0 ------0 BJEAD_156054 0 ------0 BJEAD_171059 0 ------0 BJEAD_114954 0 ------0 BJEAD_183896 0 ------0 BJEAD_114902 0 ------0 BJEAD_52991 0 ------0 BJEAD_245297 0 ------0 BJEAD_71431 0 ------0 BJEAD_171002 0 ------0 BJEAD_245049 0 ------0 BJEAD_66377 0 ------0 PHACH_6199 0 ------0 PHLBR_131358 0 ------0 PHLBR_164178 0 ------0 RHOPL_108489 0 ------0 PHACH_131961 0 ------0 RHOPL_128830 0 ------0

TRAVE_174721 1 ------MSTSSS---DPFFNFTKSSFRSAA------AQKASATSLPPLPG------PDKKVPGM 43 PHLBR_123747 1 ------MV------FYSVHDHG 10 PHACH_137275 1 ------MF------LDTTPFRA 10 BJEAD_34622 1 ------M------RLHSACQQ 9 GELSU_84792 99 LTTLPSSYVNSTHWKYVYRCQNCTTWQG----GGISLGGTGVLAWAYSNVGVDDPSDPESDFLEHTDFGFFGENFGQAE--NANYNNYVNGNPGTPTSTPPTTSPTGPTTTSPASPPTAS 212 TRAVE_73596 95 ITQLPSSSVNSTHWKFVFRCQNCTAWNG----GSIDPSGTGVFAWAFSNVAVDDPSDPNSSFAEHTDFGFFGINFPDAQ--SSNYQNYLAGNAGTPPPTSVPSGP---SSTTTTTGPTAT 205 DICSQ_153749 95 LTTLPSSLVNSTHWKYVYRCQNCTSWQGG---GGIDPTGTGVFAWAYSSVGVDDPSDPESTFQEHTDFGFFGINFPDAQ--NSNYQNYLQGNPGTPPSSTTTTTT---STSTTTTGPTAS 206 GANSP_86428 95 LTTLPSSSVNSTHWKYVFRCQNCTSWEGG---GGINPTGTGVFAWAYSNIGVDDPSDPNSTFQEHTDFGFYGINFPDAQ--NANYQNYLQGNPGTPPTTTTTTTS----TSTSTTAPTVT 205 PHLBR_160653 100 VTTISS-SINSTYWRWVFRCEGCTSWTG----GGIDVDSEGVLAWAFSNIAVDDPSDPESTFQEHTDFGFFGIDYSTAHVSSSTYSGYLNGQGGSSSPPTTSSAPPSQ-TSSAPPGPTTP 213 BJEAD_45135 100 LTTLPSSSVNSTHWKWVFRCQGCTQWSSGASSGGIDATSQGVLAWAMSSAAVDTPADPNSTFKEHTDFGFFGIDYSTTH--NANYQNYLQGNAGTPGGPSGPGTPT---TTATSTGPTVS 214 PHACH_11098 100 LTTLPETTINSTHWKWVFRCQGCTEWNNG---GGIDVTSQGVLAWAFSNVAVDDPSDPQSTFSEHTDFGFFGIDYSTAH--SANYQNYLNGDSGNPTTTSTKPTST---SSSVTTGPTVS 211 GANSP_114505 1 ------MGH 3 DICSQ_181599 1 ------MGH 3 TRAVE_144610 1 ------MGH 3 PHLBR_128980 1 ------MGQ 3 BJEAD_34705 1 ------MGH 3 PHACH_126879 1 ------MGH 3 FOMPI_127556 1 ------MH 2 GELSU_80773 1 ------MVH 3 RHOPL_118723 1 ------MH 2 WOLCO_24953 1 ------MH 2 TRAVE_43286 1 ------MASPSLH 7 DICSQ_149587 1 ------MTTPQL 6 GANSP_116439 1 ------MAAQQL 6 DICSQ_173648 1 ------MTTPQL 6 GANSP_116436 1 ------MDSPRV 6 PHLBR_157963 1 ------MSAPPLI 7 PHLBR_157964 1 ------MPAQPLI 7 PHLBR_128210 0 ------M 0 BJEAD_143000 1 ------MASAS 5 BJEAD_45314 1 ------MTSSP 5 PHACH_5574 1 ------MSS 3 BJEAD_241975 1 ------MSTTN 5 PHLBR_29466 1 ------MASA 4 RHOPL_129158 1 ------MSE 3 RHOPL_55972 0 ------0 BJEAD_227734 1 ------MASPQ 5 PHLBR_27956 1 ------MSHTPS 6 PHACH_6010 1 ------MASPS 5 DICSQ_157363 1 ------MS 2 GANSP_130292 1 ------MS 2 TRAVE_167157 1 ------MAD 3 TRAVE_170473 1 ------MT 2 RHOPL_106935 1 ------MAGA 4 WOLCO_121505 1 ------MS 2 WOLCO_132654 1 ------MANSTNP 7 FOMPI_90445 1 ------MPS 3 WOLCO_25722 1 ------MSCSS 5 FOMPI_129478 1 ------MAISL 5 FOMPI_156775 1 ------MTTS 4 DICSQ_160139 -38 ------MITPSFLQAALLFL--AVAAPQAA------YATLYQSA------DAAAAKTS -3 GANSP_124428 -18 ------MD------RAPSPTGF------HDAAAQLA -3 DICSQ_171752 -35 ------MFSHSVRAALL-----LSALSSSS------LGALLQSA------DDAADQLT -3 GANSP_67648 -30 ------MLATRLLAL--FAYCAAQ------ALSAIV------ESPTSEIL -3 DICSQ_102587 -30 ------MFSSPLLCL--YVFCITH------VLGAIF------ESPTPEIL -3 GANSP_67654 -35 ------MLGFGGILRKRPFLI---LALTTA------HAVGVV------FDGPTDIL -3 GANSP_85135 -30 ------MIRGTVLTF--AFSIVAS------ALGALY------ERPTQEVL -3 DICSQ_160546 -29 ------MLHKTGLVL---AFSVAS------VLAALH------QGPTAEVL -3 PHLBR_22550 -31 ------MGHLTALSVLFLSLI------VASRAAIY------ESPTQLPS -2 TRAVE_176148 -31 ------MLSRPLLPLGLLCLL------GQSSASLL------TDHTLVAH -2 GANSP_117498 -31 ------MLLRHSSLIGLLCFL------QQGSALLL------ADPSQVAK -2 DICSQ_182736 -31 ------MSIRRSFLIALLCFL------ENGLAALL------TDHTRLTK -2 TRAVE_40237 -40 ------MGFKCSLTGLLTLTLATIFAVQVL------PQARAALY------TDPSALPA -3 TRAVE_133945 -39 ------MGSKRSPTNLLTLTL-TLFAAAVL------PHARAALF------TDPSALPQ -3 GANSP_130042 -38 ------MFSPNLAITTLV---PLALGLVAFP------TSTRAALF------TNYADLPT -3 GANSP_138009 -31 ------MGGLA---TVALLLAAGL------GSTRAALF------TDPANLPS -3 DICSQ_103879 -32 ------MRKNLA---IFAFALAANL------ELTSAALF------TNPADVPT -3 DICSQ_96414 -32 ------MHRSFL---VVLSAVAANL------GLASAALF------TDPADILA -3 FOMPI_40728 -32 ------MRSFLALTALIAPTLW------RLTEAALY------TDPSLLPQ -2 RHOPL_55496 -32 ------MLVSLLFLSTIAASRW------SLARGALY------TDPSALPQ -2 GELSU_118493 -31 ------MRLLFDC-LLAISIWA------PTTICMLH------TNPSQLEK -2 RHOPL_54008 0 ------0 GELSU_137959 -29 ------MFWLTLCGIVLSI------QCIHSTVL------TDPAQLHK -2 GELSU_117387 -29 ------MLLPSLISALLIL------QSAQAELF------TDPSQLTK -2 GELSU_84544 -35 ------MVHCHTLRVLAAAGALLVL------PTVHAALY------TDPAKLPK -2 PHACH_37188 1 ------AQLPA 5 PHACH_135972 -34 ------MALALRP------VFASLITLLTA------TLANAALY------TDASQLPD -2 DICSQ_86071 1 ------M 1 BJEAD_156054 -32 ------MAVLR------TICVGLALSTS------LVQGATFF------TEFSQLPS -2 BJEAD_171059 -29 ------MSMSR------LLSLVLLA------TAVRGAVY------TDASQLPT -2 BJEAD_114954 0 ------0 BJEAD_183896 -29 ------MQMIW------AILWLLAA------QAALAALY------TDVNQLPG -2 BJEAD_114902 -29 ------MGL------RT-ALTFALFS------SVARGALF------TDVKQLPS -2 BJEAD_52991 -31 ------MSVLW------KC-TVLTTLLG------LAHAATPH------TVPNKQRA -2 BJEAD_245297 -29 ------MRLASPL-VA------LAGA------GLASAAVY------TDIKQLPT -2 BJEAD_71431 -36 ------MGFARSL-VQ---KIILAGVVARL------VSVRAALY------TDASQLPT -2 BJEAD_171002 -39 ------MDSSKFR-SKRFAALFASILLNSG------LTNAVTFF------TDASQLPA -2 BJEAD_245049 -38 ------MSPSSRF-SKRF-GVLLAAILANS------GVANAGLF------TDASQLPS -2 BJEAD_66377 -31 ------MRSHALVGAGLLSSL------GTVALGRI------LTSPEQIQ -2 PHACH_6199 1 ------MTDRRI------YTSFADLR 13 PHLBR_131358 -32 ------MA-PWTYFLVAALGL-SSI------IPASASIL------TSPTQIK -2 PHLBR_164178 -37 ------MSSFR-CLSQFVLLSSLLLLSP------LPVSSTLY------QSPSDLTT -2 RHOPL_108489 -48 ------MRAVSLF-----L--AA-VPLASV------AGYSTGRHPDAYHDFHERELLRRNIVYDGSIA -2 PHACH_131961 1 ------MPF------VATEDLAD 11 RHOPL_128830 -39 ------MDAPTVF-----L--VFILAVFTL------RAQTCKPLPNPPSE------SDIESFLR -2

TRAVE_174721 44 DIKYDVVIVGSGPIGCTYARELV--EAGYKVAMFDI-----GEIDS------GLKIGAHK---KNTVEYQKNIDKFVNVIQGQLMSVSVPVN 119 PHLBR_123747 11 DMETDVFIAGSGPIGAVYAKKCV--DAGLRVIMVEV-----GAARHS----PFTSDSMTSKPVVPHDASHLRSVEFRPGHVLIPGYHK---KNQIEYQKDIDRF-----GALSTVSIPTS 111 PHACH_137275 11 DEPYDVFIAGSGPIGATFAKLCV--DANLRVCMVEI-----GAA--D----SFTSKPMKGD------PNAPRSVQFGPGQVPIPGYHK---KNEIEYQKDIDRFVNVIKGALSTCSIPTS 108 BJEAD_34622 10 DIVTDVFIAGSGPIGATFAKKFV--DAGLHVVMAEI-----GAA--D----SFVSRPAKGAT--SPGGKEPYSVTFAPGEVIVPGYHK---KNEIEYQKDIDRFVNVIKGALSTVSVPSS 111 GELSU_84792 213 ATPYDYIIVGAGAGGIIAADRLS--QNNKKVLLLER-----GGPSTGETGGTYVADWAEGTN------LTKFDIPGLFESMFDDPDPW------YWCSDVT-- 294 TRAVE_73596 206 ATPFDYIVVGAGPGGLVTADRLS--EAGKKVLLLER-----GGPSTAETGGTYDATWAKSAN------LTKFDVPGLFETLFTDTNPF------WWCKDTN-- 287 DICSQ_153749 207 ATPYDYIIVGAGPGGIIAADRLS--EAGKKVLLLER-----GGPSTAETGGTYDAPWTQSAN------LTKFDVPGLFESLFTDPNDW------WWCKDIT-- 288 GANSP_86428 206 ATPYDYIIVGAGPGGIITADRLS--EAGKKVLLLER-----GGPSTAETGGTYDAPWAQSAN------LTKFDVPGLFESMFTDSNAF------WWCKDIN-- 287 PHLBR_160653 214 AVAYDYVVVGGGPGGIIAADRLS--EAGKKVLLLER-----GGPSTAETGGNYVAPWASAAGSN------LTKFDIPGLFESMFTDPDDW------WWCKDVT-- 297 BJEAD_45135 215 ATPFDYIIVGAGPAGIIAADRLS--ETGKKVLLLER-----GGPSTQETGGTYTSPWVKAAGSD------LTKFDIPGQFESMFTDTNPY------WWCKDVT-- 298 PHACH_11098 212 ATPYDYIIVGAGPGGIIAADRLS--EAGKKVLLLER-----GGPSTKQTGGTYVAPWATSSG------LTKFDIPGLFESLFTDSNPF------WWCKDIT-- 293 GANSP_114505 4 PEEVDVIVCGGGPAGSVVAGRLAYADPTLKVMLIEG-----GANNRD------DPWVYRPGIYVRNMQRDGINDKATFYVDTQKSSHLRGRRSI-- 86 DICSQ_181599 4 PEEVDVIVCGGGPAGSVVAGRLAYADPTLKVMLIEG-----GANNRD------DPWVYRPGIYVRNMQRDGVNDKATFYTDTMKSSYLRGRQAI-- 86 TRAVE_144610 4 PEEVDVIVAGGGPAGCVVAGRLAYADPTLKVMLIEG-----GANNRD------DPWVYRPGIYVRNMQRDGVNDKATFYEDSMQSSHLRGRRSI-- 86 PHLBR_128980 4 PEEVDVIVCGGGPAGCVVAGRLAYADPNLKVMLIEG-----GANNRD------DPWVYRPGIYVRNMQRNGINDKATFYTDTMESSYLRGRRSI-- 86 BJEAD_34705 4 PEEVDVIVVGGGPAGCVVAGRLAYADPDLKVMLIEG-----GANNRD------DPWVYRPGIYVRNMQKNGINDKATFYTDTQQSSHLRGRRSI-- 86 PHACH_126879 4 PEEVDVIVCGGGPAGCVVAGRLAYADPTLKVMLIEGTSGHCGANNRD------DPWVYRPGIYVRNMQRNGINDKATFYTDTMASSYLRGRRSI-- 91 FOMPI_127556 3 PEEVDVIVCGGGPAGCVVAGRLAYADPNLKVMLIEG-----GANNRD------DPWVYRPGIYVRNMQNDGVNDKATFYTDSMQSPFMRGRRSI-- 85 GELSU_80773 4 PEEVDVIVCGGGPAGCVVAGRLAYADPTLKVMLIEG-----GANNRD------DPWVYRPGIYVKNMQRDGVNDKATFYTDTMESSYMRGRKSI-- 86 RHOPL_118723 3 PEEVDVIVCGGGPAGSVVAGRLAYADPTLKVMLIEG-----GANNRD------DPWVYRPGIYVRNMQNDGVNDKATFYTDTQQSSHLRGRRSI-- 85 WOLCO_24953 3 PEEVDVIVAGGGPAGCVVAGRLAYADPTLKVMLIEG-----GSNNRD------DPWVYRPGIYVRNMQIDGVNDKATFYTDTMQSPFMRGRRSI-- 85 TRAVE_43286 8 EEEFDIILAGGGTAAGVIAGRLSSACPSLRILMLET-----GPHTQE------DLAHVQPARFLSHLRPDTIT---AKHVVARPSEHLDGRSLI-- 87 DICSQ_149587 7 FDEYDIIFAGGGTTAGIIVGRLAAADPSLRILILET-----GPHTQD------DLSHTQPARFLTHLQPGSKT---IRNVVAKPSKHLNGRELA-- 86 GANSP_116439 7 FEEYDIIFAGGGTTAGIVAGRFAAADPSLRILILEA-----GPHTQD------DLAHIQPVRYLSHLQPGSKT---VRNVIARSSEHLDGRELA-- 86 DICSQ_173648 7 FDEYDIIFAGGGTAAGVIVGRLAAADPSLRILILEI-----GPTVRD------NLAHIQPGRFLTHLLPESQT---VKHVIGKPSEHLNGRQLS-- 86 GANSP_116436 7 LDEYDIIIAGGGTAAGIIFGRLAAADPSLRILMLEI-----GPTTKD------DLAHLQPARFLTHLTPDSKT---IKHVVGKPSEHLNGRQLA-- 86 PHLBR_157963 8 ESEYDLIIAGGGTAGCIVAGRLAAAAPQLKILILEI-----GPGTKD------DPAHVQLGRFTSHYVPTAKT---IRFHFSQPSEALAGRSVP-- 87 PHLBR_157964 8 EAEYDLIVAGGGTAGCIVAGRLAAAAPDLKILVLEA-----GPSTKE------EPAHIQPARFITHYIPTAKT---VRFHVSQPSEALGGRALP-- 87 PHLBR_128210 2 PSEYDVILSGGGTASCVIAGRLADANPSLKILVVEA-----GPHTLN------EPAHVQPARFGTHLVPGSKT---MTFHVTAPEEAMGGRRSV-- 81 BJEAD_143000 6 VQEFDVIFAGGGTAACLVASRLADADPSLKILVVEA-----GPHIQD------DLAHLQPARFLSHLVPDSKV---TTYVVGNPEPQLNGIQKI-- 85 BJEAD_45314 6 PEEFDIIFAGGGTAGCLVASRLADADPTLNILVVEA-----GPHIQD------DLSHLQPARFLSHLTPGSKT---ATFMVANPEPQLGGIQKI-- 85 PHACH_5574 4 STEYDVIFAGGGTSACLIASRLADADPTLRILILEA-----GGHTQD------DLAHVQPARYLSHLAPTSRT---VSFMVANPEPALLGRQTV-- 83 BJEAD_241975 6 NAEYDLIFAGGGTTSCLVAGRLADADPTLRILIVEA-----GPHTKD------DLAHTQPARYLSHLRPDSNT---VTFVVANPEKELRDRQTI-- 85 PHLBR_29466 5 NAEFDIIFVGGGTTSCLIAGRLADADPSLKILLVEA-----GPHTKD------DLAHTQPARYLQHLVPDSKT---ITYIVANKEKELGDRQVI-- 84 RHOPL_129158 4 SLEYDVIVAGGGTVGCVIAGRLAAADPNLKILVIES-----GPPVRE------NQEHIIPAKFLSHLLPTSTT---VKFHVGKPSEELAGRTPI-- 83 RHOPL_55972 0 ------GGTSGCVIASRIADADPTKKILVVEA-----GPPVRD------DMAHIVPARYLSHVLPTSNT---LKIHVGKKSE-VLGRAPA-- 69 BJEAD_227734 6 RDEYDIVFVGAGTSGGVAAGRLAAADPSLRILMIES-----GPHVEQ------NDSFVQPARCLSHLRPDNPI---LKVHVSKVSDYLGGRPQV-- 85 PHLBR_27956 7 LAEYDIIFAGAGTSGGVAAGRLAAADPSLKILMVEA-----GPHVRE------DDAFVQPARCLTHLRADNPI---LKVHVSKPSEYLGGRSQV-- 86 PHACH_6010 6 LDEYDIIFAGAGTSGGVAAGRLAAADPSLKILLIDA-----GPHVKE------DDNFVQPAKCLSHLRPDNPI---WKVHVSNVSEYLGGRPQI-- 85 DICSQ_157363 3 HNTFDIIIAGGGTSGLIIASRLATADPSLSILVVEA-----GAPTRD------DPQHIQPVRYLHHLRPDSTT---VKFHVGRESAALGGRAPV-- 82 GANSP_130292 3 QHTFDIIVAGGGTSGLVLAGRLAAADPSLSILVVEA-----GPQTRD------DPLHLQPARYPYHLRPESTT---VKFNVGRESAALGGRAPI-- 82 TRAVE_167157 4 SATYDIIVAGGGTAGCVLAGRLAAADPSLSILVIEA-----GPTTRE------DPLHTQPARYLHHLRPESET---VKFHVGRESAALGGRAPV-- 83 TRAVE_170473 3 QATYDIIIAGGGTCGCIIAGRLAAADPSLSILVIES-----GPPTRG------DPLHIQPAHYLHHLRPDSTT---VKFQVGRESAALGGRAPI-- 82 RHOPL_106935 5 ELEFDIIVAGGGTTGCVVAGRLAAADPSLSILILEA-----GPPTHE------DLAHVQPARYLTHLLPGSQT---VKRHVGKESADVGGRAQI-- 84 WOLCO_121505 3 NMIVDVIVAGGGTAGGVIAGRLAAADPTLSILVIEA-----GPPTYE------DLAHVQPARYLTHLLPESKT---VKLYAGKYSEALGGRTPI-- 82 WOLCO_132654 8 VSDYDIVIAGGGTCACVIASRLSSADPNLRILMLEA-----GPPTHE------DLAHVQPARFLTHLRPTSTT---VKFMVGKKSNDLGGKAPI-- 87 FOMPI_90445 4 KDEYDIVIAGGGTAGLVVASRLSEADPSLSILVLEA-----GPLTYE------DPAHVQPARYLSHLLPGSKT---VRFYESKKMDQLGGRSVV-- 83 WOLCO_25722 6 GREVDIIIAGGGTSGCVIASRLAEADPSLRILILEA-----GPPTLD------DPAHIQPARYLSHLLPGSIT---TRAIVGKESEHLGGRAPV-- 85 FOMPI_129478 6 PIEADIIIAGGGTAGCVVASRLAAADPDLRILIIEA-----GPPTLE------DLAHVQPARFLSHLSPGSIT---AKFNVGKESEELGGRAPI-- 85 FOMPI_156775 5 PVEVDIIIAGGGTTGCVIAGRLAAADPSLRIAIIEA-----GPPTLD------DLAHVQPARYLSHYLPGSET---VKFNVGKESADLGGRAPI-- 84 DICSQ_160139 -2 RTKYDYIIVGAGAAGGVLANRLS-EDSTKKVLLIEA-----GSSDYN------NTNIRIPWLAP--ALTGSTF---DWNYTTTPQVGLNDRSIG-- 75 GANSP_124428 -2 STPYDYIVVGAGAAGGVLANRLT-EDGTTRVLLIEA-----GSSDYN------DTNIQIPWLAT--VLSHSQF---DWNYTTVPQEGMDNRSIA-- 75 DICSQ_171752 -2 STHYDYIIVGAGAAGGALANRLT-EDGSTKVLLIEA-----GSSDYN------NTNIEVPWLAP--VLTHSQF---DWNYTTTPQEGLDNRSIA-- 75 GANSP_67648 -2 TKNYDIIIVGAGAGGAVMANRLS-EDPRTKVLLIEA-----GGSDFM------NLNISAPGRSS--ALSRSRF---DWNFTTTPQAGLNNRSVP-- 75 DICSQ_102587 -2 NSKYDIIIIGAGAGGAVMANRLT-EDNSTKVLLIEA-----GGSDFM------NTNILAPGLAT--SLSRSKF---DWNFTTTPQVGLNNRSVQ-- 75 GANSP_67654 -2 NSIYDVVIVGGGAGGAVMASRLS-EDPGTRVLLIEA-----GSGDFN------NLNITIPGRAE--SLIRSTF---DWNFTTTPQAALNNRQIL-- 75 GANSP_85135 -2 DGTYDFIIVGAGAGGAVMASRLS-EDPHMRVLLIEA-----GGSDFE------NLNISVPGRAS--TLLRSEF---DWNFTTTPQVGLDNRPIL-- 75 DICSQ_160546 -2 NKDYDFVVVGAGAGGGVMASRLT-EDPLTRVLLIEA-----GGSDFE------NLNISVPGRSS--TLPMSAF---DWNFTTMAQPGLNNRPVG-- 75 PHLBR_22550 -1 -TKYDFIVVGGGGGGNAVANRLT-ENPDFTVLVLEA-----GVSNEG------LLDYIVPFFTV-FTRSPNPQ---DWNYTTVPQTGLNNRTLF-- 76 TRAVE_176148 -1 -KAYDFIVVGGGTAGSVLANRLT-EHAGVKVLLIEA-----GVNNAAG------PN------VDEIQIPYFVS---QIDATF---DWNYTTVAQGALQNRTIP-- 77 GANSP_117498 -1 -KSYDFVIIGGGAAGSVLAHRLT-EHSGTNVLLVEA-----GGSNTDG------PG------INDIQVPYFVV---DIPPSF---DWNYTTVPQRALQNRTLS-- 77 DICSQ_182736 -1 -KSYDFIVVGGGTAGSVLANRLT-EQPEVNVLVVEA-----GRSNIDG------PD------LDDIQVPYFVA---DIPASF---DWNYTTVPQRALQNRTLA-- 77 TRAVE_40237 -2 HKEYDYIIVGAGPSGSVLASRLT-EDARTNVLLIEA-----GPNDAG------ELSLEIPFLAA-QLQPNKEF---DWNYTTVPQAGLVGRTVP-- 76 TRAVE_133945 -2 YKAYDYIIVGAGPSGSVLASRLT-ENAHTNVLLIEA-----GPNDAG------EFDLEVPLLAS-QLQPNTAF---DWNYTTVAQSGLDGRTVP-- 76 GANSP_130042 -2 SKQYDYIVVGAGPGGSVIASRLS-ENPGTNVLLLEA-----GPSDED------VFAIQVPLLAT-GLQPNTLY---DWNYTTTPQPGLDGRDVV-- 76 GANSP_138009 -2 NQRYDYIVVGAGPGGSVVASRLS-EDSSINVLLVEA-----GPNDEG------VLPIEVPYLGY-TLQPNTTY---DWNYTTTTQTGLDGRIVL-- 76 DICSQ_103879 -2 NKQYQYVVVGAGPGGSVVASRLS-EDPHTNVLLLEA-----GPSDEG------VLQIEVPYLAL-ELQPNTTY---DWNYTTVPQTGLGGRAIA-- 76 DICSQ_96414 -2 DKQYHYIVVGAGPGGSVVASRLS-EDPQTNVLLIEA-----GPSDEG------VLPIEVPYLAY-ELQPNTTY---DWNYTTTPQTGLDGRAVI-- 76 FOMPI_40728 -1 -LRYDFIIVGAGTAGNVLANRLS-ENAAYTVLVVEA-----GGSDEE------VFAIQVPFLDS-TLSSDASI---IWNYTTTAQTGLFNRSIS-- 77 RHOPL_55496 -1 -TIYDFVIVGGGTAGNVLANRLS-EIPSFSVLVIEA-----GPSFTT------VFADEVPFLDM-TLTPDTSI---TWNYTTTEQSNLDNRIIT-- 77 GELSU_118493 -1 -TEYDFIIVGAGTAGNVIANRLT-EESSFTVLIVES-----GISNAG------VIADEIPYLAT-TLLPTSSL---TWNYTSTAQSGLNGRTIP-- 77 RHOPL_54008 1 ------AGTAGLVIANRLS-EIPSNSVLVIEA-----GISNLA------DPSDEVPFLDS-TLSPDTIL---TWNYTTTPQSGLNDRTLP-- 68 GELSU_137959 -1 -TEYDFIVVGAGTAGNVIAARLT-EEQSFSVLIIEA-----GISNED------ILETEVPLLSL-ALSPNTSV---TWNFTTTPQIGLNGRAIP-- 77 GELSU_117387 -1 -SEYDFIIVGAGTAGNVIAARLT-EDSSLDVLVVEA-----GISNVG------ILAAEVPFLGQ-TLSPDTNV---TWNFTTTPQAGLDNRVLQ-- 77 GELSU_84544 -1 -SEYDFIVIGAGTAGNVIANRLT-EEQQFSVLVIEA-----GISNEG------IIAAEVPFLGS-TLSPNSSV---TWNYTSTPQTGLNGRAIP-- 77 PHACH_37188 5 -TTFDFIVVGGGTAGNVVASRLS-EDPRHSVLLIEA-----GDWNKD------VLGVEVPFLAP-TNLANTSL---LWDYMTEPQPGLDNRTLF-- 82 PHACH_135972 -1 -NTYDFVVVGAGTAGNVMAARLS-ENPKFKVLVIEA-----GISNEG------VRNVEVPFLAT-QNIPATPV---TWNYTTVPQAGLDNRTVA-- 77 DICSQ_86071 1 -DVYDFVIVGAGAGGNVIAARLT-EDPRFSVLLIEA-----GISHEG------VLPVEIPFLAP-TSFPNTSV---TWNYSTTPQEGLNQRVLP-- 79 BJEAD_156054 -1 -STYDFIIIGAGAAGNVMAARLT-ENPKFNVLVIEA-----GISNEG------ILSLQVPFIAS-SNVPATAV---TWNYTTVPQTGLRDRVLT-- 77 BJEAD_171059 -1 -TTFDYVVVGAGSAGNVIASRLS-EDPHSSVLVIEA-----GVTNEG------VTTVAAPFLCV-KNIPFSAY---TWNFTTVPQTSLEDRALT-- 77 BJEAD_114954 1 ------AGAGGNVLARRLT-EDPNCTVLVIEA-----GISHEG------LETVESPFLLL-ENIPMSPI---TWNYTTVPQVGLNNRILT-- 68 BJEAD_183896 -1 -RSFDFVIVGAGAAGNVLARRLT-ENPSVSVLVIEA-----GISNEG------ILSIEVPFLLF-KNLPQTRV---TWNYSTVPQVGLNDRVLS-- 77 BJEAD_114902 -1 -STYDFVVVGAGSAGNVIAARLT-ENPKVSVLLIEA-----GISNDG------VLGVECPFLAP-SNLPNSSV---TWNYTTTPQTSLNNRVLG— 77 BJEAD_52991 -1 -DVFDFVIVGGGTAGNAIAARLT-EDPNCSVLIIEA-----GINNTG------VLGVDIPFLAP-ANLPNSSV---TWNYSTIPQAALNNRILT-- 77 BJEAD_245297 -1 -TTFDFVVIGGGSAGNVIASRLT-EDPRCTVLVIEA-----GGTHEG------VLSVAVPFLSV-SNLPFSEV---TWNYTTTPQAALNQRILP-- 77 BJEAD_71431 -1 -DTFDFVVIGGGAAGNVIATRLS-ENPQTSVLVIEA-----GGTNEG------VLSVEVPGLAG-ENLPFSEV---TWNFTTVPQSSLNQRVLP-- 77 BJEAD_171002 -1 -TVYDFIVVGAGNAGNVVAARLT-EDWKTTVLLIEA-----GISHEG------IDSVAVPGLVL-DNLPFSPV---MWNYTTVPQAALDNRPIP-- 77 BJEAD_245049 -1 -TTYDFVVVGAGNAGNVIAARLT-EDENTTVLLIEA-----GISHEG------ILSIAAPGLTI-QNLPFSPY---TWNYTTVPQPTLNDREIA-- 77 BJEAD_66377 -1 -ATYDFVIVGAGVGGSVLANRLT-EDASVTVLVVEA-----GGMDEG------PLGIEVPFLRP--SLAHSPL---DWNYTTVPQPSLDGRRFP-- 76 PHACH_6199 14 -PDYDFVIIGAGIGGSVLANRLS-EDPSVTVLVVEA-----GADDSD------NQQMQVPFMGV--RLAGGAA---DWKFVTTPQKGMAGRELA-- 90 PHLBR_131358 -1 -STYDFIIIGGGTAGNVLANRLS-SNKAVNVLVIEA-----GGSDVG------DVGIEVPFLGT--SLPNTPV---DWNYTTTTQLGAGFREIH-- 76 PHLBR_164178 -1 -TEYDFVIVGGGTAGLVLANRLT-EDSSVNVLVVEA-----GGSFDG------NLGIEVPFLGT--SLSGTSV---DWNYTTVPQTGYNNRTIP-- 76 RHOPL_108489 -1 -SSYDYVIVGGGTAGLVLASRLS-ENSNTSVLVLEA-----GDTGEAV------QDKIDIPSYTYYNSLVGSSY---DWAYETVAQPDADNRQIS-- 78 PHACH_131961 11 -VQPDYIVVGGGTAGLTLAARLT-EDPAITVLVVEA-----GVHHG-P------TPEIDVPGYMG-RTMTNPKF---DWTFFSVPQKRANDRVVL-- 88 RHOPL_128830 -1 -VDYDYVIIGGGTAGLALAARLS-EDPEVHVAVLEA-----GYFHL-G------DPIVDVPEYVD-EAAGNSSY---DWGFSTTPQAYAGGRNLS-- 76

TRAVE_174721 120 TLVIDTLSPTSWQAS-----SFFVRNGSNPEQDPLRNLSGQAVTRVVGGMSTH------WTCATPRFDREQ------R------PLLVKDD------T------DADDA 193 PHLBR_123747 112 NVNIPTVDPDSFQATLVSFSTPFLFMGRNPAQNPFENLGAEAVTRGVGGMTTH------WTCATPRLNPHI------ER------PVLDKDP------A------TNEK 190 PHACH_137275 109 NNHIATLDPSVVSNS---LDKPFISLGKNPAQNPFVNLGAEAVTRGVGGMSTH------WTCATPEFFAPADFNAPHRER------PKLSTDA------A------EDAR 191 BJEAD_34622 112 NENVATLDPLSFQNT---KSKPFVSLGKNPAQNPFLNLGAEAVTRGVGGMTTH------WTCATPELNPYI------ER------PVLDPDS------A------KNDQ 187 GELSU_84792 295 ------FYAGCLLGGGTSV--NGALYWYPTDTDFSTAR------GWPSSWSNHQEYTNAMT--QRLPSTDHP------350 TRAVE_73596 288 ------FFAGCLLGGGTSV--NGALYWYPNSRDFSTAS------GWPSSWGNHQPFTDKLK--QRLPSTDHP------343 DICSQ_153749 289 ------FFAGCLLGGGTSV--NGALYWYPADSDFSTEN------GWPQSWANHQPYTSKLQ--QRLPSTDHP------344 GANSP_86428 288 ------VFAGCLLGGGTSI--NGALYWYPPDSDFRTAN------GWPNSWGNHAPYTSKLK--QRLPSTDHP------343 PHLBR_160653 298 ------VFAGCLLGGGTSI--NGALYWYPNSNDFSTGV------GWPSSWTNAEPYTNQLI--ARLPSTDAP------353 BJEAD_45135 299 ------VFAGCLLGGGTSI--NGALYWYPNSADFSPSI------GWPKSWSNHGTFTNKLK--QRIPSTDHA------354 PHACH_11098 294 ------VFAGCLVGGGTSV--NGALYWYPNDGDFSSSV------GWPSSWTNHAPYTSKLS--SRLPSTDHP------349 GANSP_114505 86 ------VPCANILGGGSSI--NFQMYTRASASDWDDFK----M-EGW--TAEDLTPLMKRLE--NYQKPVNN------141 DICSQ_181599 86 ------VPCANILGGGSSI--NFQMYTRASASDWDDFK----M-EGW--TANDLLPLMKRLE--NYQKPVNN------141 TRAVE_144610 86 ------VPCANILGGGSSI--NFQMYTRASASDWDDFK----T-EGW--TAEDLFPLMKRVE--NYQKPTNN------141 PHLBR_128980 86 ------VPCANILGGGSSI--NFQMYTRASASDWDDFK----T-EGW--TAKDLLPLMKRLE--NYQKPCNN------141 BJEAD_34705 86 ------VPCANILGGGSSI--NFQMYTRASASDWDDFK----T-EGW--TCKDLHPLMKRLE--NYQKPCNN------141 PHACH_126879 91 ------VPCANILGGGSSI--NFQMYTRASASDWDDFK----T-EGW--TCKDLLPLMKRLE--NYQKPCNN------146 FOMPI_127556 85 ------VPCANILGGGSSI--NFQMYTRASASDWDDFK----T-EGW--TAQDLIPLMKRLE--RYQKPVNN------140 GELSU_80773 86 ------VPCANILGGGSSI--NFQMYTRASASDWDDFK----T-EGW--TCKDLVPLMKRLE--NYQKPVNN------141 RHOPL_118723 85 ------VPCANILGGGSSI--NFQMYTRASASDWDDFK----T-EGW--TAKDLVPLMKRLE--NYQKPANN------140 WOLCO_24953 85 ------VPCANILGGGSSI--NFQMYTRASASDWDDFK----T-EGW--TAKDLLPLMKRLE--NYQKPANN------140 TRAVE_43286 87 ------VQCGQCVGGGSSV--NFTMYTRAPASDFDDWANVYNN-PGW--AFKDVLPLIKKIE--RYQNSPGVEH------148 DICSQ_149587 86 ------VQCGQCVGGGSSV--NFAMYTRAPASDYDDWARLYDN-PGW--SWADLLPLMKKAE--TYQNAPNR------145 GANSP_116439 86 ------VQCGQCVGGGSSV--NFAMYTRAAASDYDDWASVHEN-PGW--SWADLLPLIKKAE--TYQNHPNR------145 DICSQ_173648 86 ------TQCGQCVGGGSSV--NFMKYTRAPASDYDDWAHLYNN-PGW--SFHELLSFIKKVE--TYQNVPNR------145 GANSP_116436 86 ------VQCGQCVGGGSSV--NFMMYTRAPASDYDDWAKVHDN-PGW--GFDDLLPLIKKAE--TYQVAPDQ------145 PHLBR_157963 87 ------VMAGSCLGGGSSV--NFGMYTRGSASDYDTWEQTHAN-PGW--GFKDLLPLFKKTE--TYQVKPDM------146 PHLBR_157964 87 ------VIAGSCLGGGSSINCKFGMYTRAAASDYDAWEQTYAN-PGW--GFKDLLPLFNKLE--TYQVRSNA------148 PHLBR_128210 81 ------VPCAQAVGGGSTV--NFMMYTRTSASDFDDWEKF-DN-SGW--GSKDLIPLVKKSE--TYQVAPGK------139 BJEAD_143000 85 ------VPTGHCVGGGSSI--NFMMYTRAAASDYDDWERVYGN-KGW--GSKDLIPFLKKVE--TYEVAPGK-D------145 BJEAD_45314 85 ------VPTGHCVGGGSSI--NFMMYTRASASDYDDWETVYDN-KGW--GSKELIPFLKKVE--TYEVSPGK-D------145 PHACH_5574 83 ------VPCGNCVGGGSSV--NFMMYTRAAASDYDDWEQKYEN-PGW--GAKEIIPLLKKTE--TYEVAPGR-D------143 BJEAD_241975 85 ------VPTGHCVGGGSSV--NFMMYTRAAASDYDDWENTFKN-PGW--GSKDIIPLIRKTE--TYEVAPGK-D------145 PHLBR_29466 84 ------VPTGHCVGGGSSI--NFMMYTRACASDYDDWEKKFNN-PGW--GSDVIVEMIKKTE--TYQVKPDQ-N------144 RHOPL_129158 83 ------VPAGACLGGGSSV--NFMMYTRASASDYDDWEKLYSN-PGW--GSADLLQYLKKVE--TNQALPDK------142 RHOPL_55972 69 ------VSHAQCLGGASSI--NFMMYTRASASDYDTWSRVYKN-PGW--ASADLLPLLKKYE--TYQCQEGL------128 BJEAD_227734 85 ------VTCAHAVGGGSSI--NFTMYNRAIASDFDDWEAEHKN-PGW--GAKDIVPLLKKME--TYQVEPGH-E------145 PHLBR_27956 86 ------VTCAHVVGGGSSV--NFTMYNRAVASDFDDWENIYGN-PGW--GSKDIVPLLRKTE--TYQVAPGK-E------146 PHACH_6010 85 ------VTCPHVVGGGSSV--NFTMYNRAVASDFDDWEQVHKN-PGW--GARDLVPLLKKTE--TYQVAPGK-D------145 DICSQ_157363 82 ------VPCGQCLGGGSSV--NFAMYTRAAASDYDDWTTVYGN-PGW--SSTELLPLLRKCE--TYEVAPGK------141 GANSP_130292 82 ------VPTGQCLGGGSSI--NFAMYTRAAASDYDDWAVVHGN-PGW--SSKDLLPLLRKCE--TYEVAPGK------141 TRAVE_167157 83 ------VPCGQCLGGGSSV--NFAMYTRAAASDYDDWATVYGN-KGW--SAADLLPLLRKCE--TYQLHPDK------142 TRAVE_170473 82 ------VPCGQCLGGGSSV--NFTMYTRASASDYDDWENVYGN-PGW--GSADLLPLLRKCE--TYQVEPGK------141 RHOPL_106935 84 ------VPSGHCLGGGSSV--NFAMYARAAASDYDDWANVYGN-PGW--GSEDLLPLLRKIG------K------136 WOLCO_121505 82 ------VPCGQCLGGGSSV--NFAMYARASASDYDDWESIYDN-PGW--GSRDLLPLLRKTE--TYQAQEGK------141 WOLCO_132654 87 ------VPRGACLGGGSSV--NFTMYTRPSASDFDDWEKMWGN-PGW--GCTSLMPFLKKLE--TYQAVPDL------146 FOMPI_90445 83 ------VPCGQCLGGGSSV--NFTVYARPAASDFDDWETTYGN-AGW--SSEDLIPLLKKSE--TYQAEGSP------142 WOLCO_25722 85 ------ILCGQCLGGGSSV--NFAMYARPAASDYDDWESIHGN-PGW--GSKDLIPFLKMTE--TYQVQENC------144 FOMPI_129478 85 ------VPCGQCLGGGSSI--NFTMYSRPSPSDYDEWEKVYSN-KGW--GSRDLIPLLQKME--TYQVAPDR------144 FOMPI_156775 84 ------VLSGQCLGGGSSV--NFAMYTRPSQSDYDDWETVWGN-EGW--SSRELIPLLQKME--TYQVAPGQ------143 DICSQ_160139 76 ------YARGKVLGGSTSI--NYMIYTRAAEDDYNRWASVTGD-SGW--NWNNMFKYFLKLE--DFTNSPQVVS------AST 139 GANSP_124428 76 ------YARGKVLGGSTSI--NYMIYTRGSEDDWNRFASAAVD-DGW--SWSSILPYAKKFM------126 DICSQ_171752 76 ------YARGKVLGGSTSI--NYMIYTRAAQDDWNRFASVTGD-DGW--NWSNILSYAKKLE--AFQNSPNVQN------ATS 139 GANSP_67648 76 ------YPRGFVLGGSTAI--NLMIYSRGTIDDFNRFADVSGD-KGW--SWTRLLPFIFKVD--KMTAPTDGHN------TTG 139 DICSQ_102587 76 ------YPRGFVLGGSTAI--NLMIYSRGTIDDFNRVANVSGD-EGW--SWNEMLPFIFKVD--NMTTPTDGHN------TTG 139 GANSP_67654 76 ------YPRGFVLGGSTAI--NIMAFCRGTVDDYDRWANVTGD-EGW--SWKSLTPFILKVD--RMTAPVDHHN------PSG 139 GANSP_85135 76 ------YPRGFVLGGSTAI--NQMAFCRGTEDDYDRWANVTGD-DGW--SWKKLLPFILQVD--RMTAPADRHD------TTG 139 DICSQ_160546 76 ------YPRGFVLGGSTAL--NQMVFSRGTKDDYDRWANVTGD-DGW--SFKNLLPFIFAVD--RMTAPVDHHN------TIG 139 PHLBR_22550 77 ------YLRAKMLGGCTSH--NGMAYVRGSRDDWDRYAWVTGD-PGW--SWDAIQPYIRKNE--HWMPPTDHHN------TTG 140 TRAVE_176148 78 ------YPRGHVLGGSTST--NFMFYTRGSSDEFNKLARVSGD-DGW--SWKQILPYILKTE--TLTPPADGHN------TVG 141 GANSP_117498 78 ------YPRGHVLGGSTST--NFMFYTRGSSDEFDRLARISGD-SGW--SWKAMLPYIRKTE--TLTPPADGHD------TRG 141 DICSQ_182736 78 ------YPRGHVLGGSSST--NFMFYTRGSSDEFDRLARISGD-EGW--SWKAMLPYILKSE--TLTPPADDHN------TTG 141 TRAVE_40237 77 ------YSRGRVLGGSTSI--NFMIYTRGSKDDFNNYAALSGD--DW--SWDALQPYFKKLE--NLVPPVDGHD------TSG 139 TRAVE_133945 77 ------YPRGRVLGGGTSI--NFMIYTRGSKDDFDNYAALTGD-EGW--SWNALQPYFKKLE--HLVPPADGHD------TTG 140 GANSP_130042 77 ------YPRGRVLGGSSSI--NLMIWTRSSQDDFDRYATVSGD-SGW--SWDAIEPYYKKAE--QFVPPADHHD------TSN 140 GANSP_138009 77 ------YPRGRVLGGSSSI--NLMVWTRSSRDDFDRYASFSGD-SGW--SWDAVEPYFRKIE--HLVPPVDHHN------TSG 140 DICSQ_103879 77 ------YTRGHVLGGSSSI--NIMAWTRSSLDDFNRYAAVSGD-DGW--SWDAIEPYFKKIE--HFVPPVDHHN------TSG 140 DICSQ_96414 77 ------YSRGRVLGGSSSI--NVMAYTRSSRDDFDRYAAVGGD-QGW--SWDAIEPYYRKLE--HLVPPVDHHN------TSG 140 FOMPI_40728 78 ------YPRGRVLGGSSSI--NYMVWTRGSKDDWDRYAEFTED-DGW--SWDAMLPYMIKSE--RLVPPSSGRN------TTG 140 RHOPL_55496 78 ------YPRGRVLGGSSVV--NLLVWTRGSEDDYNRLAAATGE-SGW--GWNSMLDYMMKSE--ELVPPSDHMD------TAG 140 GELSU_118493 78 ------YPRGRALGGSSTI--NLQAWTRASQDDWNRFASFTGD-NGW--SWDAMLPYMKKSE--SLVAPPDHHN------TSG 140 RHOPL_54008 69 ------YPRGRVLGGSSTI--NFQIWTRGSVDDYNRYANVTGD-PGW--SWNEILPYMKKSE--SLVPPSDHHN------TTG 132 GELSU_137959 78 ------YPRGRVLGGSSTI--NFMIWTRASEDDWDRFAESTGD-LGW--SWDAMLPYMKKSE--QLVLPADRHN------TTG 140 GELSU_117387 78 ------YPRGRVLGGSSTI--NFEIWTRASEDDWNRFANVTGD-NGW--SWNEILPYMKKSE--SLVAPADHHN------TTG 140 GELSU_84544 78 ------YPRGRVLGGSSTI--NFEIWTRASKDDWDRFAEFTGD-EGW--SWDSILPFMKKSE--SLVASTDHHN------TAG 140 PHACH_37188 83 ------YPRGKLLGGSSSI--NFLAYTRGADEEYDQWAELTGD-AGW--SWENMSQYYYKNS--RLVPPADGHD------TTG 146 PHACH_135972 78 ------YPRGRVLGGSSSI--NFLAYTRGSDAEFDRWANLTGD-EGW--SWANLLPYYLKNA--RLVPPADHHN------TSG 140 DICSQ_86071 80 ------YPRGRVLGGSTSI--NFMAYTRGSNEEFDRWAAITKD-DGW--SWKNVEIYYLRNS--KLVPRADNQS------CAG 142 BJEAD_156054 78 ------YPRGRVVGGTASI--NFMTYTRGSNDEYDRWATLTGD-SGW--SWKNLSPYYFKTT--RLAPSVDGHD------TTG 140 BJEAD_171059 78 ------YARGRILGGSSSL--NFMTYTRGSNDDYDRWANLTED-DTW--SWDNLNDYYLRSS--RLVPAKD-QS------NVG 139 BJEAD_114954 69 ------YPRGRLLGGSTSI--NFMTYTRGANEDYDRWANITHD-KGW--AWDNLDKYYRRVSFVRLVPSEG-RN------TTG 133 BJEAD_183896 78 ------YSRGRVLGGSSSI--NFMTYTRGSDADYDRWANLTGD-DGW--SWRNLEKYYRRSS--RLAPTPG-RN------TTG 169 BJEAD_114902 78 ------YSRGRVLGGSSSI--NFMTYTRGSDAEYDRWAQLTGD-SGW--SWSNVSTYYFKSS--QLVASAR-N------TTG 138 BJEAD_52991 78 ------YPRGRVLGGSSSI--NFFTYTRGADEEYDRWANITGD-EGW--AWKNVAPFYLKSS--KLVPSQQ-GS------LAG 139 BJEAD_245297 78 ------YSRGRVLGGSSSL--NFMVYTRGANDEYDRWANLTGD-DGW--KWENVEQFYFKNS--RLVPPQDGHN------TTG 140 BJEAD_71431 78 ------YSRGRILGGSSSL--NFMVYTRGADAEYDRWAQITGD-SGW--AWENLAPYYLKNS--RLTPPQDGHN------TTG 140 BJEAD_171002 78 ------YAKGRVVGGSSSV--NFMEYTRSSNTEYDRWANLTGD-AGW--SWKNMEQYYLKNS--RLVPPQDGHN------TTG 140 BJEAD_245049 78 ------YTKGRIIGGSSSV--NFMAYTRGADDEYDRWANLTGD-DGW--AWKNMEAYHLKNE--RLVPPRDGHN------TTG 140 BJEAD_66377 77 ------FSRGKAIGGTTVI--NFHTWNIGSNDTWDRWADITDD-QAW--AWKNAQTYFRRTS--HYAHLPDGSSYV-----QPVEE 143 PHACH_6199 91 ------CARGRTLGGSTRI--NLMTWNNGGAELWDHWADITGD-KGW--SWKSAQHYYKKAC--HLVPPADGRD------TQG 154 PHLBR_131358 77 ------YTRGKVLGGSSCL--NLLTWNICSNDLWNHWASLTGD-SGW--AWDNVKQYYLKTS--SLVPPADGHN------DAG 139 PHLBR_164178 77 ------YARGHVLSGSSSI--NLLTYNRGSNGVWDRLADASGD-AGW--SWDSMEPYYMMSS--RLVPPADDHN------TTG 139 RHOPL_108489 79 ------WPRGKVLGGSSAI--NGMYLVRPSELEINAWASLVPNGSIW--NWDNMYAAMKKSE--TYTPPLSNVQ------DTAKI 145 PHACH_131961 89 ------QPRGKGLGGSSLI--NYLGIFRPSKIELDAIEELG-N-PGW--NWDSLLHYMKKAR--NAAECLQPPDVSRTNAL----K 156 RHOPL_128830 77 ------LPRGKMLGGSSGI--NGMAWNRASRAEYDAWRTFAPE-NDW--TWDGLLPFFKKSE--NLSLSPSDPYPGITPMEKAQAV 149

TRAVE_174721 194 EWDRLYTKAESYFKTGTDQFKESIRHNLVLNKLAEEY---KGQRDFQQIPLAATR-RSPTFVEW------SSANTVFDLQNRPNTDAPNERFNLFPAVACERVVRNT---- 290 PHLBR_123747 191 IWDDLYTEAEEIIGTSRTQFNKSIRQTLVLEALGE-----TPNRAFVPLPLACHRLDDPGYVEW------HATDRILEELF--TDLEKSKRFTLMTNHICTKVSMVP---- 284 PHACH_137275 192 IWKDLYAQAKEIIGTSTTEFDHSIRHNLVLRKYNDIFQKENVIREFSPLPLACHRLTDPDYVEW------HATDRILEELF--TDPVKRGRFTLLTNHRCTKLVFKH---- 290 BJEAD_34622 188 LWAALYKEARALIGTSEKEFDQSIRHQLVLQTYQ---KNHGTSRVFKPLPLACHRLDDREYVEW------HATDRILETLF--TDPVKRAKFTLLTNHRCTRVIPTH---- 283 GELSU_84792 351 ----STDGE------RYLEQSAQVAM-QLLNAQGYY------QATINDSPDSKDH----VYGYS------AFDFINGKRGGVVATYL-QTANQRSNFVYKDYTLVSSVVRNG---- 434 TRAVE_73596 344 ----SADGQ------RYLEQSATVVQ-QLLSGQGYS------QITINDNPDSKDH----VFGFS------AFDFLNGQRAGPVATYF-ETALARKNFVYKDNVLVTQVIRNG---- 427 DICSQ_153749 345 ----STDGK------RYLEESANVVV-QLLSKQGYS------NITINDNPNSKDH----VYGYS------AFDFINGERAGPVATYF-QTAKARPNFTYKDYVLVSQVVRNG---- 428 GANSP_86428 344 ----STDGK------RYLEESAAIVA-QLLNGQGYS------NITINDNPDSKDH----VYGYS------AFDFIGGERGGPVATYF-QTAKARPNFTYKQYVLVSQVIRNG---- 429 PHLBR_160653 354 ----SVDGK------RYLEESATLVG-QFLSSQGYS------QITINDNPNYKDH----VYGYS------AFDFIGGKRGGPVATYF-QTASARPNFTYKDFVLVSNVVRNG---- 437 BJEAD_45135 355 ----STDGK------RYLTQSADVVM-SMLKNQGYQ------NITINDNPDYKDH----AMGYS------AFTFIDGKRGGPVATYF-QTASARKNLVYKDFTLVTNVVRNG---- 438 PHACH_11098 350 ----STDGQ------RYLEQSFNVVS-QLLKGQGYN------QATINDNPNYKDH----VFGYS------AFDFLNGKRAGPVATYL-QTALARPNFTFKTNVMVSNVVRNG---- 433 GANSP_114505 141 ----DTHGYDGPIAISNGGQIQPVAQ-DFLRAAHAI------GVPYSDDIQDLKT--AHGAEIW------AKYINRHTGRRSDAATAYVHSVMDVQSNLFLRCNARVSRVLFDA---- 236 DICSQ_181599 141 ----DTHGYDGPIAISNGGQIQPVAQ-DFLRAAHAI------GVPYSDDIQDLKT--AHGAEIW------AKYINRHTGRRSDAATAYVHSVMDVQSNLYLRCNARVNRVLFDG---- 236 TRAVE_144610 141 ----DTHGYDGPIAISNGGQITPVAQ-DFLRASHAI------GIPYSDDIQDLKT--SHGAEIW------AKYINRHTGRRSDAATAYVHSVMDVQNNLFLRCNARVSRVLFDG---- 236 PHLBR_128980 141 ----DTHGYDGPIAISNGGQITPVAQ-DFLRASHAI------GVPFSD----LDT--AHGAEIW------AKYVNRHTGRRSDAATAYVHSVMDVQSNLYLRTNARVSRVLFDA---- 232 BJEAD_34705 141 ----DTHGYDGPIGISNGGQIMPVAQ-DFLRASHAI------GIPYSDDIQDLTT--AHGAEIW------AKYINRHTGRRSDAATAYVHSVMDVQTNLYLRCNARGSRVLFDA---- 236 PHACH_126879 146 ----DTHGYDGPIAISNGGQIMPVAQ-DFLRAAHAIAM----LTPCTDDIQDLTT--AHGAEIW------AKYINPHTGRRSDAATAYVHSVMDVQDNLFLRCNARVSRVLFDD---- 243 FOMPI_127556 140 ----DTHGYDGPIAISNGGQIQKVAH-DFLRASHAV------GIPFSDDIQDLKT--AHAAEIW------AKYINRETGRRSDAATAYVHSVQDVQDNLYLRTDARVSRVLFDA---- 235 GELSU_80773 141 ----DTHGYDGPIAISNGGQITPVAQ-DFLRASHAI------GVPFSDDIQDLTT--AHGAEIW------AKYINRHTGRRSDAATAYVHSVMDVQDNLYLRTNARVSRVLFDA---- 236 RHOPL_118723 140 ----DTHGYDGPIAISNGGQIQPVAQ-DFLRASHAI------GIPYSDDIQDLNT--AHGAEIW------AKYINRHTGRRSDAATAYVHSVMDVQDNLYLRCNARVNRVLFDA---- 235 WOLCO_24953 140 ----DTHGYDGPIAISNGGQIQPVAQ-DFLRAAHAI------GVPFSDDIQDLTT--GHGAEIW------AKYINRHTGRRSDAATAYVHSVMDVQDNLYLRCNARVSRILFDA---- 235 TRAVE_43286 148 ----HTHGYDGPLKVSYGGYRNAVGE-DYLATVAQYDT----GRPIVNDINTMVE-DVNRFERY------PKWIDEQTGRRSDVPHHYVYNNASYGSNLQLRAACTVKRVIMEN---- 246 DICSQ_149587 145 ----PTHGYSGPLKVSYGGKRTNVGE-DYIATVSKYDK----TRVIVDDANRM------FW------PKWIDGNSGHRSDVAHNYLYQVLKTGTNVHLLPGYFVKKVIIED---- 235 GANSP_116439 145 ----PTHGYSGPLKVSYGGAPTNVGL-DYMATVGKYDQ----TRAVVDDANRMVD-DVNRHEFW------PKWIDGKTGRRSDVAHNYLYNVLATSTNVHLLAGHFVKRIIIED---- 243 DICSQ_173648 145 ----PTHGYDGPLKVSYGGALTSVGT-DFVETVLRYDR----TRTAVDDANSMVQ-DVNRIEPW------PKWIDGKTGRRSDVAHHYIYPALDKSKNVHLLPGCAVKRVVIEE---- 243 GANSP_116436 145 ----PTHGYNGPLKVSYGGARTHIGE-DFISTIVQYDK----TRAVVDDANGMIM-DVNGLQPW------PKWIDGKTGRRSDVAHNYIYPALERSNNVRLLAGCAVKKVIIEG---- 243 PHLBR_157963 146 ----PTHGYSGPLKVSYGSLCTDIGK-EFLSAIGQTDPNR--SGDSSRDATDFHE--VNVYNQW------AKWSDEKTGRRSDVPHHYIYNQEA-NTNLHVVTGVNVLRVVLED---- 244 PHLBR_157964 148 ----PNHGNSGPLKVSYGGVYTDIGK-QFSQTVGQVDPKR--PGDNSRDATDFYE--VNVFNRW------AKWIDEESGRRSDVAHHYIYNQD--SKNLHVVTGASATRVIFEG---- 245 PHLBR_128210 139 ----DTHGYSGPLRVSYGSFFTNIGR-DFLDVAPKYDT----SRGLTEDPNTMHA-----EGIN------RWVDAETGTRSDTAHHYLYKKNH--SNVTIITGHLVKRVVFEY---- 230 BJEAD_143000 145 ----ETHGYSGPLKVSYGGWFSNVGK-DYLEVGTKYE-----KRGISDDGNGLFE--CDKYARW------PKWIGQKTGMRSDVAHHFIYNKTR--KNLTLVSGYLVKRVIFEY---- 239 BJEAD_45314 145 ----ETHGYSGPLKVSYGGWFSNVGK-DYLEAGSKYE-----KREFCEDGNGPFE--SNKYARW------PKWIGQKTGVRSDVAHHFIYDKTR--KNLSVVSGYLVKRVLFEG---- 239 PHACH_5574 143 ----DVHGYSGPLRVSYGGIFTNVGK-HFLEVGAAYDP----QRGTTDDPNNLYD--VDKYGRW------QKWISAKTGTRSDVPHHFIYNKKH--PNITLTVGHLVKRVLFED---- 238 BJEAD_241975 145 ----TVHGYSGPLKVSYGGAFTSVGK-DFLDIGPKYDT----KRGAIDDPNNLFE--VDKFGRW------QKWIDGKTGKRSDVPHHFIYNKDH--KNITLLTGQLVKRVIFEG---- 240 PHLBR_29466 144 ----HNHGYTGPLKVSYGGIFPNVGK-DFLAVGEKYGVKHDPTRVSTDDPNGLFEPYVNKFGRW------QKWISAEDGKRQDVPHHFIYNKSH--PNITITTGHLVKRVIFEN---- 245 RHOPL_129158 142 ----PTHGYEGPLKVSFGGIYADAGK-QFLEIAEQFDK----DRPVVDDMNDLSN--SNAYARW------AKWIDCKTGRRQDVPHNYIYNQID-TTGLDVRTGYTVKRILFDG---- 238 RHOPL_55972 128 ----ETHGYSGPLKVSYGGIQTEIGT-QFLEVASGLL-----KRPVVDDFNDLRQ--ANAVSRW------PKWINNKTGRRQDVPHNYLYDKDR-TTGLDILTGFVVKRVLVEN---- 223 BJEAD_227734 145 ----DVHGYTGPLKVSHGGKFTNIAQ-DFLAVAKDFDK----TRAHTEDLNMIHN--SNQYGRM------QKWIDGETGKRSDVAHHYIYNNMK-KTGLEILTGTLVRRVLFDG---- 241 PHLBR_27956 146 ----DVHGYTGPLGVSYGGKFTNIGQ-DFLDVAAKFDK----SRSIIEDPNGVYT--SNAYGRM------QKWIDGKTGKRSDVAHNYIYNH----PSIEILTDSLVKRIIFEG---- 239 PHACH_6010 145 ----DVHGYDGPLKVSYGGKFTNIGQ-DFLEVAKGFDP----AREHTEDPNQIYK--SNAYGK------WIDGVTGKRSDVAHHYLYNNTK-NTGLEILTGTLVKRVIFEG---- 238 DICSQ_157363 141 ----PTHGYSGPLKVSYGGAFMDIGK-EFLEVAAGYDK----ARGLTDDVNGLFE--TNKYGVSS------VSWIDSKTGARSDIPHQYIYHRDF--PNLHILTGYHVQRVIVKD---- 237 GANSP_130292 141 ----PTHGYSGPLKVSYGGAFLEAAK-EFMEVAAQYDS----TRGFTDDINGLFE--TNKYGVD------YRWIDSKTGVRSDIPHHYIYHRDL--KNLHILTGYHVKRVVVEG---- 236 TRAVE_167157 142 ----PTHGYSGPLKVSYGGFSSEVGE-EFLNVAAQYDK----TRGLTDDVNNLYD--CNKYGKW------QKWIDAKTGTRSDVPHNYIYPQ-SANTNLNILTGHHVKQVILKD---- 238 TRAVE_170473 141 ----PTHGYSGPLKISQGGFFTETGK-EFLDVAAQYDK----TRGQTDDVNGLFE--CDKYGVART----ITCRWIDAKTGTRSDVPHHYIYNQDFREDQVRILTGYDVKKILFEG---- 242 RHOPL_106935 136 ----ETHGYSGPLKVSYGGHFTNVGQ-QWLQVATQYDK----ERTVTDDPNDLHT--CNAYGFHWVTMPMCDIRWIDAETGRRSDVPHHYIYGRGL--RNLTIMTGWNVKRVLIEN---- 239 WOLCO_121505 141 ----PNHGYSGPLGVSYGGQFTNLGQ-QWLGVATQYDK----ERTFTDDPNGLYS--CNSYGSLTI------HRWINARTGRRSDVPHNYLYNQAE-NKNLKILTSHHVKRIIFEYER-- 241 WOLCO_132654 146 ----PTHGYDGPLKVSLTRTPNVLAD-EFLAVASQYDT----YRSYTNDPSGIFD--VNAYARW------PRWIDEKTGRRSDVPHHYLYNQGE-KKNLEIKTGHLVKRVIFEG---- 242 FOMPI_90445 142 ----DTHGYVGPLKVSYGGLYTNVGQ-QFLEVAARYDK----KRTLIDDPNGLFK--CNAYGRW------QKYISADDGRRSDVPHHYIYNRDF--PKLAILTGYLVKRVIFEG---- 237 WOLCO_25722 144 ----ATHGYSGPLGISYGGLFTNVGK-QFLEVAAQYDT----ARTTTDDINGMYE--CNAYGRW------QKFIDARTGRRSDVPHNYLYNHNY--QNVEILTGYLVKRVLFEG---- 239 FOMPI_129478 144 ----PTHGYEGPLNISHGGHFTSIAR-DFLEVGAQYDT----GRGSTDDPNDMIG--IN------AYINEDTGGRSDVPHNYIYNHQF--DNLEILTGYHIKRVLVED---- 233 FOMPI_156775 143 ----PTHGYDGPLKISHGGVYTNVGK-QFLDVAVQYDK----TRGTTDDPSDMIG--INAYGRW------QKYIDKEKGHRSDVPHNYIYNRQL--DNLEILTGYHVKRVIIEN---- 238 DICSQ_160139 140 KFVPSLHNTTGPLGAGLPEVTLATDN-IGLQAQQQLSA----EFAYNQDVNSGNM-----IGFSWT----PF---SIQ-NGARSDSARDYI-QPALSRSNLDVIVNAQVTKVVQTTTS-- 238 GANSP_124428 137 ---AAVHGYNGPAGAGLPQVSLAIDQ-LGLNAQQELVS----EFKYNQDVNSGDM-----IGFSAS----IDFRSRIQ-DGARSNSARDYI-QPALPRSNLDVLVNTQVIKVLKTGAD-- 235 DICSQ_171752 140 KFVANIHGTNGPVGAGLPQVSLPIDQ-IGLNAQQELSS----EFKYNQDVNSGNM-----IGFSWT----PF---AIQ-DGARSNSARDYI-QPALGRSNLDVLVNTQVTKVLQTGTD-- 238 GANSP_67648 140 QFIPALHSTRGVVDISVPGESLAIDV-RGLGASAELSQ----QFPFNLDYNSGDT-----IGLSWT----QS---TIH-SGRRVTSATSYL-AEAFNRTNLDVLVNTRVTKIIPVGSV-- 238 DICSQ_102587 140 QFNPALHNTDGVVDISVPGVSLDIDA-RGLNASQELPD----EFPFNLDYNSGNT-----TGFSWT----QA---TIH-DGRRTTSATSYL-AEAFNRTNLDILVNTRVTKIAPVGEV-- 238 GANSP_67654 140 QFNPALHN-DGVVPISVEGFSLGTDS-RVIETTQELPD----QFPFNLDYNSGDT-----VGFGWI----QS---TIQ-NGRRVSAATSYL-AAALARPNLDVVVNTRVTKVLPVGYE-- 237 GANSP_85135 140 QFNPAIHK-NGVVPISVEGFPLEINP-RVINTTQELAE----QFPFNLDYNSGDT-----IGFGWT----QS---TIH-DGHRVTSATSYL-ARAFDRPNLDILVNTRVTKVLPVGRE-- 237 DICSQ_160546 140 QFNPAIHK-NGVVPISVEGFPLEINS-RVIDTTRELAE----QFPFNLDYNSGNT-----IGFGWT----QN---TIH-DGHRVTSASSYL-AQAINRPNLDVVVNTRVTKVVSVGRE-- 237 PHLBR_22550 141 QFNPAVHGFHGITAVTLPGFKQEIDP-LMLEVTQQLND----SFPFNLDSNSGYP-----LGIGWG----PA---TTR-HGRRDSSATSYLGPQFIGRPNLHVLLHAQVTRLVQTAS--- 239 TRAVE_176148 142 QVDARIHGKSGPVLTSLPGNASVLDS-RVIQTTSQLS-----EFPFNLDMNSGNP-----LGVGWL----QS---TIG-HGVRSSAATAFLTEETLKRSNLDVLIGTRVTRLIQSKS--S 240 GANSP_117498 142 QVNPAVHGTTGPVLTSLPGNASVLDT-RVLQTTAELA-----EFPFNLDMNSGNP-----LGVGWL----QS---TIG-HGVRSSAATAFLTQDTLNRKNLDVLIGTRVTRLLRTGW--D 240 DICSQ_182736 142 QVDPAVHGKTGPVLTSLPGNASVLDS-RVLQTTSELS-----EFPFNLDMNSGNP-----LGVGWL----QS---TIG-HGVRSSAATAFLAQETINRKNLDVLIGTRVTRLLQTER--K 238 TRAVE_40237 140 EILPNIHGTSGPLKISLPGVPLPSDP-RVIQATQELRS----EFPFNVDMSSGDP-----LGIGWS----PG---TFG-GGARVSASVAYL-QPVLGRRNMDVLVNTTVTKLIHTGT--E 238 TRAVE_133945 141 EVLPSIHGTSGPLNISLPGAPLPSDS-RVIQTTQELSS----EFPFNEDMSSGNP-----LGIGWM----QG---TFG-GGARVSASVAYL-QPVLGRSNLDVLVDTTVTKLTQTGT--K 239 GANSP_130042 141 EINIGIHGTCGPVNITLPNAPYPIDS-RVNATLGELSD----QYPYNKDMNSGNP-----LGVGWT----QS---TYG-NGVRSSASSSYI-RPVMSRPNLDVLVNTVVTKVINTGRDAL 241 GANSP_138009 141 QVDFGIHGHHGPVNITVANVPFPIDP-HVIGTTQDLSE----EFPYNGDMNSGDP-----IGIGWT----QG---TYG-NGVRSSSTSSYI-RPVLTRPNLDVLVNTIATKVITIGPEAE 241 DICSQ_103879 141 QIDIQIHGTRGPLNITVNNTPYPIDS-HVINTTQELSD----EYPFNIDMNSGHP-----LGIGWA----QS---ATG-NGMRSSASSSYI-RPVLSRPNLDVLVNTVATRLLRSGHDNV 241 DICSQ_96414 141 QVEWQIHGTRGPLNITVANAPFPIDS-HVVATTQELSE----EYPYNEDMNSGYP-----LGIGWA----QA---TYG-NGIRATASSSYI-RPVISRPNLDVLINTITTKILRSRHSDE 241 FOMPI_40728 141 EIDVSLHGHKGPVNISLASATTPIDG-RILETTHDLPQ----LFPYNLDMNSGDP-----LGVGYT----QS---SITSAGRRANSAVSYL-RPALERPNLHVLIQTQVTKLVASETL-V 241 RHOPL_55496 141 KVDSSVHGTNGPIHISLEGYPVNIDQ-RIIGMTNLTPP----VFDYVEDMNAGFP-----IGLGWA----QS---AIGTNGHRSDSATGYL-DPVLGRANLHILAETTVTKLISTGT--R 241 GELSU_118493 141 EIIPSLHGTSGPVQISLGGFPMEIDQ-RVFDTTEDLPS----EFPFNEDMNSGSP-----LGIGRL----PF---SVDTEGRRSSSATAYL-QPVLNRSNIDVLVMTQVTKLIASGT--T 240 RHOPL_54008 133 QVDPLVHGTSGPVQISLAGYSTGIDQ-RVIETSHELQH----EFPYNIDYNAGYP-----LGLGWS----VG---AVSTSGRRSSSATAYL-DPILSRSNLDVLIQTQVTKLVSQNS--T 232 GELSU_137959 141 EIEPDFHGFHGPIDIGLPGFSFETDP-LVINTTKELPD----EFPFNEDMNSGFP-----LGVGWT----QS---SIDSQGRRSSSATGYL-GPALNRSNLDVLISTQVTRLISTRT--V 240 GELSU_117387 141 EVNPAVHGTNGPIQVSLPGFPSEIDQ-LIINTTEELPS----AFPFNEDVNSGVP-----LGVGWT----QG---SIGTNGHRSSSATAYL-EPVLNRANLDVLIMTTVTKLVTSSN--A 240 GELSU_84544 141 EVDQSVHGHSGPIQVSLGGFPTEIDQ-RITNTTKQFPN----EFPFNIDMNSGTP-----LGVGWT----QD---SIGTDGHRSSSATGYL-SPALNRSNLDVLITTTVTQLLTSGAK-V 241 PHACH_37188 147 QVDPAHHGY-GPLEISVPGYPSEIDD-MVIGTSKAPGA----EVPYNLDVNSGHP-----LGLSYL----QS---TVG-RGARSSSATAYI-HPSLGRKNLHVLVSHTVTRLMPVGST-- 244 PHACH_135972 141 EVIPADHGY-GPLEMSLPGFPTTADQ-KVVATTQVNGS----LFPYNIDLNSGNS-----VGIGLI----QS---TIG-HGERSSSATAYL-EPALNRPNLHLLIENTVTRLVSTKGS-- 238 DICSQ_86071 143 LVNPADHGF-GPVDVSLPGFPTEIDG-RFVDASKSSGS----QFPFSIDLNSGNA-----LGFEGV---VQN---AIG-RGARSSSATAYL-EPALNRSNLHVLIENTVTKLISTGSV-- 241 BJEAD_156054 141 QVDPKVHGN-GPVDVSVYNFASKTDS-LVVGTGKT-DP----EFPFNLDIQSGNP-----LGVSYA----QS---TVG-NGERSNSATGYL-TPALKRKNLHVLIQNTVTRLVSTGSD-- 237 BJEAD_171059 140 LVDPAVHGY-GPIEVSISDFRTELDA-RIVNTSKT-DP----EFPFNLDFQSGNS-----VGVAVA----QS---TIG-NGTRSSSATAYL-EPALSRRNLHVLIENTVTRLMPTGKE-- 236 BJEAD_114954 134 LVDPAAHGF-GPVEVSISDFVSELDD-RVVNTSKT-VP----EFPFNIDFQSGNS-----VGVSLA----QS---AIS-NGTRSSSATAYL-EPALNRTNLHVLIQNTVTKLIHTGFE-- 230 BJEAD_183896 170 LVDPAVHGH-GPVEVSLSSVVSELDN-RVLNTSMT-VP----EFPFNLDIQSGNS-----VGVSLE----QC---TIG-NGTRSSSAVAYL-EPALRRKNLHVLVQHTVTRLISTGHE-- 236 BJEAD_114902 139 LVDPNDHGN-GPIDVSLSDFPSQLDD-RVVQTAKT-TA----EFPFNIDFQSGNS-----VGVSPA----QN---TIG-HGVRSSSATAYL-EPALGRSNLDVLITNTVTRLIATGNV-- 235 BJEAD_52991 140 NFDSRFHGN-GPVEVSLPDFPTELDE-RVRNTAMT-TP----EFPFNIDFQSGNP-----IGVSAL---PQF---AIG-NGVRSSSATAYL-DPALHRPNLHVVTERTVTRLIQTGTN-- 237 BJEAD_245297 141 QVIPEVHGF-GPVEISLPDFPSVLDQ-RIIDTASAPGA----EFPFQEDFQAGDG-----TGISLV----QS---TVG-NGARSSSAVAYL-APALNRPNMHVLLHNTVTRLVKTGSS-- 238 BJEAD_71431 141 QVNPLVHGD-GPIEVSLPGFPSVLDN-RILDTVRAPGA----EFPFIEDFQSGNS-----VGVALV----QS---TIG-DGARSSSAAAYL-QPAISRSNLYVLVQNTVTRLVQTEST-- 238 BJEAD_171002 141 DVDPSVHGY-GPVPLSLVGTPNPLDA-RMRAATQAPGA----EFPFQLDMNGGDM-----IGFGIV----QS---TIL-NGERMHSGKAFL-EPAMSRPNLHVLIENTVTRLIQTKTT-- 238 BJEAD_245049 141 QVEPSVHGN-GPIPISLAGWPNPVDP-RILETVQAPAT----VFPFQPDMNAGST-----IGFGIV----QS---TVD-MGERIHSGIAFL-EPALKRPNLDVLIENQVTRLIQTGF--- 237 BJEAD_66377 144 FVNPDVYGD-GPIGVSAPGQFLPLDE-AVVRASKERSG----KWSWNRDANGGNG-----VGVMMM----QN---SVN-GGKRNDAVTTYL-RPAANRTNLDILINTHVTKVLPSVTNKN 242 PHACH_6199 155 FVEPSAHGD-GPLSVSVPGYSTPLDK-IIFDAMGGLGG----TWSFNSDFNAGTC-----LGTGYM----QS---SIG-SGERSDAASAYL-YPASTRPNVDILVNTQVTKLIPLPG--- 251 PHLBR_131358 140 EVIPSVHGD-GPVDVSVPGFPLPLDS-LVINASHQLGG----RWTYNEDINAGNG-----LGTAYM----QS---SVG-GGQRSHAATAYL-HPVDNRTNLDILINTQVTRILRSGT-D- 237 PHLBR_164178 140 EVIPSAHGD-GPVQVSLAGFPLEIDN-YVMNASMELDG----RFAFNEDFNDGCT-----VGISYI----QN---TIG-GGERSSSGTAYY-DPVQNRTNLHVLINTRATRLINTAAGD- 238 RHOPL_108489 146 EYSNSSHGFSGPLHASYPGYMMPQVG-DWTPSLNNI------DIMTSPNPDGGDG-----WGAFVA---TSA---INPANWTRSYSRSAYI-DPLPPRANLAILPNATVTRIVFANA--- 243 PHACH_131961 157 YAMKPDLEYHGPIKNSFGPVLGEMHM-KLFDSAQAL------GIPRNPETGNGRN-----DGCMTS---LLS---VDATTAKRSYAASAYL-EPNLDRSNLLVLTEAYVTKVLLEEN--- 254 RHOPL_128830 150 KILPKVDGFSGPIVASYNSHYFEDAP-LLVETLNAI------GIPTNPDSQSGNS-----TGIFNT---LAS---LDRRTGTRSYAAMGYY-CDQPVRANLHILVDAQVTKIHFVEG--- 247

TRAVE_174721 291 ----SNSEIESLHIHDLISG------DRFEIKADVFVLTA--GAVHNAQLLVNSGFGQL------GRPDPANPPRLLPSLGSYITEQSLVFCQTVMSTELIDSVKSDMI 381 PHLBR_123747 285 RLKGP------GGEQDQYEAYVIAC--GAVGTAQVLANSQRPEPLLD------GENEDDKTIIETPLTPNLGSYITEQPMTFCQVVLNADIIAKVDTE-- 368 PHACH_137275 291 YRPGEENEVDYALVEDLLPHMQNPGNPASVKKIYARSYVVAC--GAVATAQVLANSHIPPDDVVIPFPGGEKGSGGGERDATIPTPLMPMLGKYITEQPMTFCQVVLDSSLMEVVRNP-- 406 BJEAD_34622 284 FNPGQPNEVGYAEVESLLPRTEGRRGSST-FAIRAKIYVIAC--GAVGSAQVLANSNVNPETKGPFYD-----PNRDMTEDIIDTRLMPNLGRYMTEQPMTFCQVVLNSGLINSVDDD-- 393 GELSU_84792 435 ---SQILGVQTNNTA-IGPN------GFIPLNPNGRVILSA--GSFGTPRILFQSGIGPTDMLQTVQQNAAVA--ANMPAESDWINLP-VGMNVSDNPSINLVFTHPS--IDAYDNW-- 534 TRAVE_73596 428 ---STILGVRTNDNT-LGPD------GIVPLNPNGRVILSG--GSFGTPRILFQSGIGPTDMLQTVQSNAQAA--ANLPPQSEWINLP-VGQSVSDNPSINLVFTHPS--IDAYDNW-- 527 DICSQ_153749 429 ---ATITGVRTNDTS-LGPN------GIVPLNPNGRVILSA--GSFGTPRILFQSGIGPTDMIQAVQANPTAG--ANLPAQSDWINLP-VGQGVSDNPSINLVFTHPS--IDAYENW-- 528 GANSP_86428 430 ---STITGVRTNDTS-LGPN------GIIPLNPNGRVVLSA--GSFGTPRILFQSGIGPSDMLQTVQSNPTAN--ANLPPQSQWIDLP-VGQGVSDNPSINLVFTHPS--IDAYDNW-- 527 PHLBR_160653 438 ---SQITGVQTNDTS-LGPN------GVVPLTPNGRVILSA--GSFGTPRILFQSGIGPSDMISLVQGNADAA--TSLPPANEFIDLP-VGMNVQDNPSINLVFTHPS--INSYDNW-- 537 BJEAD_45135 439 ---SQITGVQTNDTS-LGPN------GIIPLTPNGRVILSA--GSFGTPRILIQSGIGPSDQIQLVQNNADAA--SRLPPSNQFINLP-VGFNAQDNPSINLVFTHPS--IDAYDNW-- 538 PHACH_11098 434 ---SQILGVQTNDPT-LGPN------GFIPVTPKGRVILSA--GAFGTSRILFQSGIGPTDMIQTVQSNPTAA--AALPPQNQWINLP-VGMNAQDNPSINLVFTHPS--IDAYENW-- 533 GANSP_114505 236 --NNKAVGVAYVPSRNRANNADV----LETVVKARKMVVLSS--GTLGTPQILERSGVGNAEMLNKLNIPVV------SDLPGVGEEYQDHYTTLSIYRVSN-ETQTLDDF-- 332 DICSQ_181599 236 --NNKAVGVAYVPSRNRANNGQV----VETIVKARKMVVLSS--GTLGTPQILERSGVGNAELLNKLNIPVV------SDLPGVGEEYQDHYTTLSIYRVSN-ETSTLDDF-- 332 TRAVE_144610 236 --NNKAVGVAYVPSRNRTHGGQV----QETIVKARKMVVLSS--GTLGTPQILERSGVGNAELLNKLNIPVV------SDLPGVGEEYQDHYTTLSVYRVSN-ETQTLDDF-- 332 PHLBR_128980 232 --NNKAVGVAYVPAKGRTHNNEI----QETVVKARKMVVLSS--GTLGTPQILERSGVGNGDLLRQLGVKVV------SDLPGVGEEYQDHYTTLTLYRVSN-ETVTTDDF-- 328 BJEAD_34705 236 --NNKAVGVAYVPSRNRANAGEV----QETIVKARKMVVISS--GTLGTPQILERSGVGNGELLNQLGIKVV------SDLPGVGEQYQDHYTTLSIYRVSN-ETMTADDF-- 332 PHACH_126879 243 --NNKAVGVAYVPSRNRTHGGKL----HETIVKARKMVVLSS--GTLGTPQILERSGVGNGELLRQLGIKIV------SDLPGVGEQYQDHYTTLSIYRVSN-ESITTDDF-- 339 FOMPI_127556 235 --NNKAVGVAYVPSRNRASGGKV----QETIVKARKMVVISS--GTLGTPQILERSGVGNAELLRDLGIKVV------SDLPGVGEEYQDHYTTLSLFRVSN-ETETTDDF-- 331 GELSU_80773 236 --NNKAVGVAYVPSRNRAHGGKV----VETIVKARKMVVLSS--GTLGTPQILERSGVGNGEMLNQLGIKVV------SDLPGVGEEYQDHYTTLSIYRVSN-DTLTTDDF-- 332 RHOPL_118723 235 --NNKAVGVAYVPSRNRTSGGKV----HETIVKARKMVVLSS--GTLGTPQILERSGVGNAELLSQLGIKVV------SDLPGVGEEYQDHYTTLSLYRVSN-DTQTTDDF-- 331 WOLCO_24953 235 --NNKAVGVAYVPSRNRTHGGKV----QETIVKARKMVVLSS--GTLGTPQILERSGVGNAELLRSLDIKVV------SDLPGVGEEYQDHYTTLTLYRVSN-ETETTDDF-- 331 TRAVE_43286 246 ---GRAVGVEYMHNPRINADADN----VLHIAKAKRMVIVSA--GTFGSPGILERSGIGAPGILKQNGVEPI------VDLPGVGENYNDHPLLFLPFYAAE-GTVTLDGI-- 341 DICSQ_149587 235 ---GRATGVEFVRNAQVFPDVDG----TIRVAQSKQLVVLSA--GAFGSAAILERSGIGRKDVLERNGIEQK------VNLPMVGENYNDHHLFFLNFHASE-DAETIDGI-- 330 GANSP_116439 243 ---GRAAGVEFVRNAQVLPDASD----APRTARAKKLVVLSA--GGFGSPAILERSGIGKKDVLERSGIKQV------VDLPMVGENYNDHHLMFLPFHASE-DAETIDGV-- 338 DICSQ_173648 243 ---GKATGVEFLHNILLSSDADT----TPRIVRASKLVVISA--GTFGSPGILERSGIGSKDILDRNSIKQV------VDLPGVGENYNDHPLIFVPFHAKQ-DADTLDGI-- 338 GANSP_116436 243 ---GKATGVEYVLNSQVLPGADK----MPRIARATKLVILSA--GAFGSPGILERSGIGAKDILARNDIKQV------VDLPGVGENYNDHPLIFIPFHAKE-DADTLDGI-- 338 PHLBR_157963 244 ---NRAVGVEYQWNSTVLSDVDC----EVHTVRARKMVVISS--GAFGTPAILERSGIGGKAVLEKLNIPIK------VDLEGVGIDYQDHNIMFIPYYARS-DTKTLDAL-- 339 PHLBR_157964 245 ---DRAVGVEYRWNHGVLPEADS----DVHTVRASRMVVLSS--GTFGSPAILERSGIGAKSLLEKLEIPVK------VNLEGVGHNYQDHNIVFTPYIASA-ETLTFDAI-- 340 PHLBR_128210 230 ---KRAVGIEYVENPRMYPNTTG----EVTFVRARRLVVVSA--GSLGSPGVLERSGIGAKSVLEKCGVKPI------ADLPGVGENYQDHNILFNACVASE-DSQTLDPI-- 325 BJEAD_143000 239 ---NRAVGIEYVENPRVYPNATTS---KVTTARARRLVVVSG--GSLGSPAILERSGIGAKSVLEKAGVKQI------VDLPGVGSDYQDHLVMFTPFLASS-ETSSIDAI-- 335 BJEAD_45314 239 ---TRAVGVEYVENPRVYPNATG----KVTTARAKRLVVVSG--GSLGSPAILERSGIGAKEVLDKAGVEQI------VELPGVGSEYQDHLVLFTPFLASP-ETSSIDGI-- 334 PHACH_5574 238 ---KRAVGVEYVQNKKVFPEAKP----DILVAKAKRLVVLSA--GTFGSPVILERSGIGAKDVLEKAGIPQL------VDLPGVGENYQDHQVIFAPYLASE-DSETIDGI-- 333 BJEAD_241975 240 ---KRAVGVEYQQNPRVYPNATK----DVVIARAKRLVVVSA--GTFGSPALLERSGIGAKKVLEKYGVPQL------VDLPGVGENYQDHQVIFAPYLASE-DADTIDGI-- 335 PHLBR_29466 245 ---GRAVGVEYVENPRVYPNATK----DVLIAKATRLVVVSA--GTFGTPGVLERSGIGAKHILEKYGVKQI------VDLPGVGENYQDHQVIFSPYLASE-ESDTIDAI-- 340 RHOPL_129158 238 ---TRATGVEFVPNARYNPDEDK----TPRIAKARKLVVLSA--GTFGTPSVLERSGVGAEKRLSALGVKTV------SDLPGVGENYQDHPIVFSQYFVDD-NADTLDGL-- 333 RHOPL_55972 223 ---GRAVGIEFLPDSRFHPQETQ----TPRIAKARRLVVLSA--GTFGSPVILERSGIGGAERLEKLGVDMV------ADLPGVGENYQDHPVVFTQYFAGD-EADTLDGL-- 318 BJEAD_227734 241 ---TRATGVEYVPNPIFQPNASME----GRTVRATKLVVISA--GTFGSPGVLERSGIGKRDVLEAAGVPVK------VDLPGVGENYQDHPTIFAPYLATP-DSDTLDGI-- 336 PHLBR_27956 239 ---KRAVGIEYVPNPVFQPNASPE----AKVVRAKKLVVVSA--GTFGSPGILERSGIGGKDVLEKAGVKQV------VDLPGVGENYQDHSTIFAPYLATA-DSFTLDGI-- 334 PHACH_6010 238 ---KRAVGVEYVANPIFQPEASKD---LVRTVRAKRLVVVSA--GTFGSPAILERSGIGAKDVLEKAGVPQL------VDLPGVGEHYQDHSTIFAPYLATA-DSFTLDGI-- 334 DICSQ_157363 237 ---GQATGIEYSPNKRYHSDAGQ----GVLSAIARRQVVISA--GTFGSPAILERSGIGSKSVLEKVGIMQI------VDLPGVGENYQDHQVLFAPYVIAK-EKDTLDGI-- 332 GANSP_130292 236 ---GRATGVEYIPNKRFHPHASL----EVISATARRLVIISA--GAFGSPAILERSGIGAKTVLTKVGVKQI------IDLPGVGENYQDHQTVFPPYAVAE-GTDTLDGI-- 331 TRAVE_167157 238 ---GVAVGVQYIPNTRFHPDAKI----EVLTALARRLVVLSA--GAFGSPAILERSGVGSGETLDKVGVKQV------VDLPGVGESYQDHQLILPPYISAT-TVNTLDAI-- 333 TRAVE_170473 242 ---KRAVGVEYIANSRFNPDAKP----EVLTATARRLVVISA--GAFGSPAILERSGVGSKEALAKVGVEQI------IDLPGVGESYQDHQVIFGPYVAAD-DVHTLDGI-- 337 RHOPL_106935 239 ---SRAVGVEFVPNKQLNPDTTQ----MVRTARARRMVVLSA--GAFGPGALGDRQREAAPGAWDRVSC------RPSRYHIVLFTPYHVAD-EAETLDGI-- 324 WOLCO_121505 241 --NKRAVGIEYIPNISVNQELLP----QVHTAHARRLIVVSA--GAFGSPAILERSGIGSPSILKKLEIDVK------VDLPGVGQNYQDHNVLFTPYLADD-NEDTLDSI-- 337 WOLCO_132654 242 ---TRAVGVEYVPDKNVSPDVPQ----QVYVARARRMVIVSA--GTFGSPSILERSGIGAASLLQGLGISTL------VDLPGVGENYQDHQGLASPYLVDE-SVPTIDGI-- 337 FOMPI_90445 237 ---ARAVGVEYSANARFHPDEKR----GIQVARARKLVLVSA--GAFGSPAILERSGIGSSELLNRLQIEAV------VALPGVGDKYQDHQVVFAPYLVRD-EAETLDGI-- 332 WOLCO_25722 239 ---ERAVGVEYLPNTRFRPDEAAS---GERIVRARILVVVSA--GAFGSPGILERSGIGTSSLLVPLGITPL------VDLPGVGENYQDHNVIFAPYLADE-EAETIDGI-- 335 FOMPI_129478 233 ---GRATGVEILPNPLIRPTEEQ----TPRIAQARKLVLVSS--GAFGSPAILERSGIGQRARLQSLGIDVV------SDLPGVGENFQDHYVTYVPYIADN-SAETLDGI-- 328 FOMPI_156775 238 ---GRATGVEFLPNARFRPNEEQ----TIRTARARKLVIVSS--GTLGSPVILERSGIGERNRLQALGIDVV------VDVPAVGENFQDHNVIFAPYLADD-EAETLDGI-- 333 DICSQ_160139 239 GKTPVVRTVQFQTGS------GG----KSYSLTANREVILSA--GAIGSPQILLLSGIGPVAQSKSLGIKSL------VDLPDVGQNLQDHPLLTTNFQVSS--SDTLDNL-- 329 GANSP_124428 236 GGTPIVNGVQITTGP------NE----KRYNLTAAKEVILSA--GATGTPHILLLSGIGPADQLKTLSIESI------VDLPNVGQNMQDHPLLTTNFRVSS--NDTLDNL-- 316 DICSQ_171752 239 GNTPIVNAVQFTSGP------NE----KLYNLTANKEVILSA--GSIGTPQILLLSGIGPADQAKSLGIQSI------VDLPDVGQNMQDHPLLTTNFQVSS--NDTLDNL-- 329 GANSP_67648 239 NGAPDMRGVQFAQTA------NG----TVHTLKAAKEVILSA--GSIKSPHILMLSGIGNREHLSPFGIETV------VDLPGVGMNLQDHVFLGNSWLVNA--NFTLDDL-- 329 DICSQ_102587 239 NGVPDLRSVQFAQSA------NG----TLYTLEAAEEVILSA--GAVQSPHILMLSGIGNKDHISSFGIKTL------VDLPAVGTNMQDHVFLGNSWLVNS--NFTLDDL-- 329 GANSP_67654 238 EGKPVFRGAQLAQTA------DG----PIYTLNATKEVILSA--GSTNTPHILLLSGIGDSMHLSSFGIETL------VDLPSVGQNLQDHPFLANTWMVNS--SNTLDDL-- 328 GANSP_85135 238 DGRPVFRSVQFAQTA------DG----D--VQLATKEVILSA--GSLNTPHILMLSGIGDTSYLQSVGIKPI------VDLPAVGQNLHDHAFLGNNWIVNS--NNTLDNL-- 326 DICSQ_160546 238 QGKPVFRGVEIAQSA------DG----PVHTLKAKKEVILSA--GSLKTPHILMLSGIGDAAHLTALGIKPV------VNLPAVGQNLQDHVFLGNSWVANS--TNTLDDL-- 328 PHLBR_22550 240 ------KA----PKLQVTATKEVILSG--GAIGTPQILLNSGIGDAKELAAVGITPR------VHLPSVGKNLSVHVGAPVIHFVNS--TGTFDDV-- 315 TRAVE_176148 241 SATPTFLGVELAQTA------TG----PRVQLTAAKEVILSA--GSIGTPQILMLSGIGDKAELSAHKLATV------VELPDVGKNMQDHPWVPLQWQVNS--NDTLDTI-- 331 GANSP_117498 241 EGRPVFLGVELAQSA------AG----ARVHIKAAKEVILSA--GSIGTPQILMMSGIGDRAELAAHGIATL------VDAPDVGKHMQDHPWVPLQWQVNT--NDTLDSI-- 329 DICSQ_182736 239 DGKPVFLGVELAQCD------SG----TKVQIRAKKEVILSA--GSIGTPQILMLSGIGGKEELTRLGITTV------VDAPDVGKHMQDHPWVPLQWQVNT--TNTLETI-- 331 TRAVE_40237 239 YGQPVFREVQFAQSS------SA----PIFVLKAIKEVILAA--GAINTPQLLMLSGIGSAQTLRSLRIKPV------LDLPDVGQHLADHPLVTSQFGVTQSSDDIIDNF-- 331 TRAVE_133945 240 DGEPVFRGVQFAQSS------SA----PKFALNATQEVILAA--GSVNTPQLLMLSGIGPAKALKALGIKPI------LDLPDVGQHLADHPFLTNQFGVADSSDDLIDNV-- 332 GANSP_130042 242 TGLPVFSGVEVAQSA------TS----SVRAINATKEVILSA--GAINTPQILLLSGIGPSSPLAALGIKKT------VNIPAVGQNLVDHLLVTNQFNVAAPEDDFLESI-- 334 GANSP_138009 242 DGFPAFTGVEVARTS------AS----PTFTLMATKEIILSA--GAINTPQLLMLSGIGPSSHLASVGTKTL------VENPTVGQHLADHPRVANQFGVAATEDDLYDAI-- 334 DICSQ_103879 242 TGVPVFSGVEVAQSS------TS----PVFAFNATKEVILSA--GAVNTPQLLMLSGIGPSSHLSSLGIETV------VDHPSVGQNLSDHPGVGNQYSVAAAQDDTDDNL-- 334 DICSQ_96414 242 SGVPVFSGVEVAQSS------TS----PTFAFNATKEVILSA--GAINTPQLLLLSGIGPRAHLASLGIETV------VDLPAVGQNLMDHPRVGNQFSVASAQDDPGDTI-- 334 FOMPI_40728 242 NGTAAFTSVEVAKNA------SS----DRIVFQANKEVILSA--GAIGTPQILQLSGIGDPDKLRSVGITPL------VNLSDVGENLVDQPILGSQWFANS--TATNDQI-- 332 RHOPL_55496 242 NNEPVFTTIQMAQSS------TS----QTYTVHASKEIILSA--GSINTPQLMMLSGIGDSATLKSLAIESI------VNSPGVGQNLIDHPLITNNWLVNS--TNTNDEY-- 331 GELSU_118493 241 DGMPHFDAVQIAQSP------TS----PRFQISAKKEVILCA--GAVNTPQILQLSGVGDPTHLRAMGIEPV------VELSDVGQNLVDHPFLTLQWFANA--QGTTDVV-- 331 RHOPL_54008 233 GGSLSFRTVEMAQSS------TS----ERYTVSATKEVILSA--GTIGTAQILQLSGIGDPALLESVGVTPL------VNSPNVGQHLTDHPLLSNQWLVNT--SFTLDNV-- 323 GELSU_137959 241 DRLPNFNTVEVAQSP------TG----KRMNFTARKEVIVSA--GSVGTPQLLQLSGIGNATLLRSVDVKPI------VELDDVGQHLTDHPFLGNQWFSKS--NDSLESA-- 331 GELSU_117387 241 SGVPRFDTVEIAQSA------SS----QKFQVTATKEIILSA--GSVQTPQLLQLSGIGNSTLLSAVGITPL------VELPDVGQHLADHPFLGNHWFVNS--TDTLESV-- 331 GELSU_84544 242 KGQPHFDIVEMAQTP------TS----HRFTVRAANEIILSA--GSTNTPQLLLLSGIGPEAQLRAHGITPI------VNAPDVGQHLADHPFLGNHFFVNS--TSTLEGI-- 332 PHACH_37188 245 SGGPSFRRLSSTLKS------XA----KRSTATARKEVILSA--GAVNSPQLLMLSGIGDRQALASVGVTPV------VHLPDVGQHLSDHPYVSNYWTVSS--NMTLDNV-- 335 PHACH_135972 239 NGVPTFTGVEFAASA------SA----PRHTVTVKKEIILSA--GSIGTPQILLLSGVGNKTDLASIGIPSV------VDLPDVGQNLKDHPILSNYWQVTM--NDTFDDV-- 329 DICSQ_86071 242 KGAPSFRKVQFAPNA------TA----PYSLVTARKEVILSAVFPDVGTPQILLLSGIGDKDRLRALDIEPL------LDLPDVGQHLKDHPILANYWNVSS--NNTYDDL-- 334 BJEAD_156054 238 KGVPVFKSVEFAASE------QG----KRFTVTATKEIILSA--GSVGTPQILLLSGIGDAKELSKVGIKST------VNLPDVGKHLQDHPLVSNFFAVNS--NNTFDDV-- 328 BJEAD_171059 237 HGLPTFKKVEFAPSA------DA----HRSVVTANKEVILSA--GAIGSPQILMLSGIGDHDELAALGIKPQ------VNLPEVGKHLQDHPIMANYFTVTS--NDTFDDT-- 327 BJEAD_114954 231 NGLPTFKKVEFAPNA------DS----PRSTVYAKKEVLLCG--GAVNTPQLLMLSGIGDREEIIAMGIQPH------VHLPDVGKHLQDHPFVANYFTVNS--TTTFDTT-- 321 BJEAD_183896 237 KGLPTFKTVEFAQSS------DS----PRYRVSAAKEVILSA--GAVNTPQILMLSGIGDKEELASLGIRPL------VHIPDVGKHLQDHPIAANYFEVTT--ADVFDSA-- 327 BJEAD_114902 236 NRKPSFKKVEFAPSA------QA----ARSTVTARKEVVLSA--GSIGTPQILMLSGIGDANDLSGLNIPTS------VNLPDVGKNLQDHPILSNYFTVNS--NTTFDNV-- 326 BJEAD_52991 238 HGTPEFTVVEFAASQ------NG----PRQNVTARREIILAA--GAVNTPQVLMLSGIGDTSELIQFGIKPR------VHSPDVGKHLQDHPIMSNYWLVNS--TNTFDDV-- 328 BJEAD_245297 239 HGVPSFKKVEFAPSA------SA----KRSTVTARREVVLSA--GTIGTPQILMLSGIGNKTELAKVGVEST------VELPDVGHNLKDHPIMSNYFLVNT--TNTPDEF-- 329 BJEAD_71431 239 RNGPSFKTVEFAASA------SG----RRHTVTANNEVILSA--GTIGTAQILMLSGIGDREVLDTAGITTV------VELPDVGKNLKDHPILSNYWVANS--TETGDDT-- 329 BJEAD_171002 239 NGLPSFMKVEFARDA------LS----ARSVVTATKEVVLSA--GSIGTPQILMLSGIGDREELTAIGVNVT------VDNPAVGKHLKDHPIMANYFQVNS--NETYDDI-- 329 BJEAD_245049 238 GDTPSFMNVEFARSA------TS----KHTVVTAKKEVVLSA--GSMGTPHILMHSGIGDKDELTAVGINST------VHLPEVGKNLKDHPIMANYFEVNS--NDTYDDV-- 328 BJEAD_66377 243 TGEVLFSIVEMAQGP------TA----PRQIVEATKEIILAG--GVFNTPQILQLSGIGPRTLLESHGIPVH------IDNPEVGAHFSEHPMVPLYFRVNS--TETADSV-- 334 PHACH_6199 252 SELPDLRLVELAQTR------EG----PRHTVKASKEVLLAA--GVIGSPQILQLSGVGPADVLTAHGVPVL------VDNSSVGANYQEHVMVGLHFEVNS--PETWDPV-- 342 PHLBR_131358 238 GRIPAFREVEMAQSS------AG----QRYTVKATKEVLLAA--GVFGSPQILQLSGIGPQRVLRSVGITPI------VYNNDVGAGLADHPLVPLYYEVNS--NATWDNV-- 328 PHLBR_164178 239 EQTPVFDTVELEQ------SVLVNATNEVVLAA--GTIATPQLLQLSGVGDAGFLTGLGIEPV------VDLPDVGANLQDHPMAVAYLQVNS--SKGWDDV-- 324 RHOPL_108489 244 TGGLTATGVQYASQK------GA----TESTVSVNKEVILAA--GAIGSPQILMVSGVGPQDVLEAAGVAVQ------VALPGVGQHLQDHLYAEVTWKTNE--QTAGSMF-- 334 PHACH_131961 255 GDLRRAVGIEYLKGG------I-----QLRIDNVKRDVIVAA--GTLQTPQILELSGIGNPTILSQFGIETI------IDLPGVGENLQDHVGVTTIVEVNT--R------338 RHOPL_128830 248 EELVIATAVQFAVGP------T------SYVVNASREVILSA--GTIQTPQILELSDILRNNATFAAE------301

TRAVE_174721 382 IRGNPGDP------GYSVTYTPGA------STNKHPDWWNEK------VKNHMMQHQEDPLP-I--PFEDPEP------QVTTLF 439 PHLBR_123747 369 ------PHRPDWWVQG------VEDHIRQYPQDPIH-I--PFRDPEP------QITSPF 406 PHACH_137275 407 ------PWPGLDWWKEK------VARHVEAFPNDPIP-I--PFRDPEP------QVTIKF 445 BJEAD_34622 394 ------LENKPAWWREG------VEKHKADNPEDPIP-I--PFRDPEP------QVTTPF 432 GELSU_84792 535 ADVWTDPR------PADAAQYLAS-QSGVFAGASP--KLNFWRDYEGSDGI------QRSAQG-TVRPGAASV- 591 TRAVE_73596 528 ADVWSNPR------PADAQQYLQS-RSGVLAGASP--KLNFWRAYGGSDGI------TRYAQG-TVRPGAASV- 584 DICSQ_153749 529 ADVWSDPR------PADAQQYLTS-RSGVFAGASP--KLNFWRAYGGNDGF------TRYAQG-TVRPGAASV- 585 GANSP_86428 528 ADVWSDPR------PADAQQYLKD-RSGVFAGASP--KLNFWRAYGASDGV------TRYAQG-TVRPGAASV- 584 PHLBR_160653 538 ADVWTDPP------SADAQQYLSS-FSGILAGASP--KLNFWRAYSGGNI------TYYAQG-TVRPGAASV- 593 BJEAD_45135 539 ATVWTGPR------LADQQQYLKD-QSGVLASASP--KLNFWRAYSGSDGN------QRWAQG-TVRPGAASI- 595 PHACH_11098 534 ADVWSNPR------PADAAQYLAN-QSGVFAGASP--KLNFWRAYSGSDGF------TRYAQG-TVRPGAASV- 590 GANSP_114505 333 LRGDKDTQ------KELFAEWETSPEKARLASNAIDAGFKIRPTAAELQEMGP--EFN--ALWERYFKDKPDKPVMF 399 DICSQ_181599 333 LRGDKEVQ------KELFAEWETSPEKARLSSNAIDAGFKIRPTEEELKEMGP--EFN--ELWDRYFKDKPDKPVMF 399 TRAVE_144610 333 LRGVKDVQ------KELFAEWETSPTKARLSSNAIDAGFKIRPNEKELKEMGP--EFN--ELWDRYFKDKPDKPVMF 399 PHLBR_128980 329 LRGVKEVQ------KELFAEWETSPEKARLSSNFIDAGFKIRPTAEELKEMGP--EFN--ELWNRYFKDKPDKPVMF 395 BJEAD_34705 333 LRGVKEVQ------RELFQEWETSPEKARLSSNAIDAGFKIRPTEAELKEMGP--EFN--ELWDRYFKDKPDKPVMF 399 PHACH_126879 340 LRGVKDVQ------RELFTEWEVSPEKARLSSNAIDAGFKIRPTEEELKEMGP--EFN--ELWNRYFKDKPDKPVMF 406 FOMPI_127556 332 LRGVKEAQ------HELFSEWEQSPEKARLSSNSIDAGFKIRPTEEELKEMGP--EFN--ELFNRYFKDKPDKPVMF 398 GELSU_80773 333 LRGVKEVQ------QEIFQEWEVSPEKARLSSNSIDAGFKIRPTEEELKEMGP--EFN--ELWDRYFKDKPDKPVMF 399 RHOPL_118723 332 LRGVKEAQ------QEIFQEWETSPEKARLSSNSIDAGFKIRPTEEELKEMGP--EFN--ELWNRYFKDKPDKPVMF 398 WOLCO_24953 332 LRGVKEVQ------DKLFQEWETSPEKARLSSNCIDAGWKVRPTEEELKEMGP--EFN--ELWNRYFKDKPDKPVMF 398 TRAVE_43286 342 IRNDKEEI------EKWSTQWVSE-GKGLMATNGIDAGCKYRPTPAELEDIGP--TFK--ERWESFYANSPEKSILW 407 DICSQ_149587 331 VRNDPGEI------EKWSAQWSKD-GKGLMATNGCDGGMKYRPSPEELHKIGP--DFADSDLWRSFYAPSPDKPVLF 398 GANSP_116439 339 VRSDPNEI------QKWSAQWLED-GKGLMATNGCDGGMKYRPSPEELRAIGP--DFADSEIWKSFYAPSPDKAVLF 406 DICSQ_173648 339 VRGDADEL------EKWSAQWQQN-GQGLMATNGLDGGYKFRPSEKELRTIGP--EFA--ERWASYYASTPDRPIMW 404 GANSP_116436 339 VRGDPSEL------AKWSAKWLQD-NQGLMASNGVDGGMKSRPSEQELHAIGP--EFA--ERWASYYAGAPDKPIIW 404 PHLBR_157963 340 FRSEPDAV------TEAEEEWLKS-GQGLLATNGVDGGGRLRPSEQELEEWGP--EFK--QRWATEFAPYLDKAALW 405 PHLBR_157964 341 MRCEPDAV------AEAQEEWLAT-GKGLIATNGVDCGGKIRPTAQELEEWGV--EFK--KRWEAEFAPYLDKAPLW 406 PHLBR_128210 326 VRQTEPEY------STIVEWNEQWLKD-GTGLMTNNSVDAGIRLRPDERELEQIGP--EFT--ETWNELYANAPDKPVMW 394 BJEAD_143000 336 VRNEEPHF------EKWSKQWKQD-GSGLMATNSIDVAMRLRPTPEELKAIGP--EFQ--QRWNMYYAPSPDKPILL 401 BJEAD_45314 335 VRNEEPHF------EKWSKQWMAD-GTGLMATNSIDVAIRLRPTAEELKTIGP--DFH--QRWNTYFASAPDKPTTV 400 PHACH_5574 334 VRSEEAEI------TKWSAQWKQD-GSGLMSSNALDAGVKIRPTPDELKAIGP--AFT--QKWTEYYVPAPDKAVMW 399 BJEAD_241975 336 VRNNEADY------GKWSTQWLQD-GQGLMSSNALDAGVKLRPTADELAVIGP--AFT--EKWKQYYENVPDKPVMW 401 PHLBR_29466 341 VRSEEPVF------TTTSERWLKD-GQGLMSSNGLDAGVKIRPTPEELKAIGP--AFT--KKWEDYYLNAPDKPVMW 406 RHOPL_129158 334 IQGRPEEL------QKWGAQYMTD-GTGLLAHNAVEAGGKLRPSPQDLDAFGP--DFR--KRWDEFYAKYPDKPVLF 399 RHOPL_55972 319 MQGKPDEV------HRWTEQWAKD-GTGMLASNGIEAGSQLRLTPEDLKTLGP--EVQ--QRWAHDFADVSDKSIMC 384 BJEAD_227734 337 GRGDPLEV------KKFHEEWQKT-GKGMMAHNGLDAGLKLRPTEEELKELGP--TFQ--KRWKEYFEDKPDKPVLF 402 PHLBR_27956 335 GRGDPEEV------AKWHTQWLKD-GQGMMAHNGLDAGIKLRPNEEELKEFGP--EFK--QRWKKYFADKPDKPVLF 400 PHACH_6010 335 GRGDPEE------TASAGLDAGIKWRPSEQELQELGP--AFA--ERWKEYFADKPDKPVIF 385 DICSQ_157363 333 ASSNEAEL------QKWDAQWKKQ-GDGLMAHNGLDAGGKLRPSKDDLDVLGE--VFR--KRWLEYYVPAPDKPVMW 398 GANSP_130292 332 ATSDEAEL------QKWGAQWEKQ-RDGLMAHNGLDAGGKLRPLKEDLDVIGE--AFR--KRWLEYYVPSPDKPVIW 397 TRAVE_167157 334 TENDEAEI------KKWTKQWKEN-GSGLLATNGLEAGIKIRPSRKDLDVIGD--EFR--KRWLDYYAPAPDKPVLW 399 TRAVE_170473 338 VTDDKAEI------AKWTKEWKET-GSGLIAHNGLDAGVKMRPNEAELSQLGE--KFR--QRWQEFYVPAPDKPVLW 403 RHOPL_106935 325 VRSEESE------LNKCGIDAGVKLRPSERELAIIGP--EFR--QLWSEYYANAPDKPVMW 375 WOLCO_121505 338 LRGEPAEI------EKWSTQWLKD-GTGLMAHNGIDAGIKIRPSERELGTIGP--EFE--KRWASFYANAPDKPVMF 403 WOLCO_132654 338 VRGNTEEI------AKWTAQWLKD-GSGLMASNAIDAGVKYRPSDEELKEIGP--DFK--QHWNEYFADSPDKPVIW 403 FOMPI_90445 333 VRGDPDEV------EKWHAQWLKD-GTGLMAHNGIDAAGKLRPSPEELYTIGP--EFR--ERWETYFKDAPDKPVIW 398 WOLCO_25722 336 ARSDPQEL------DRWNSVWLES-GTGLMAHNSIDSGIKLRPTEEELKEIGP--DFE--HQWKNFYADAPDKPALW 401 FOMPI_129478 329 TRNDPTEI------ERWSTQWLKD-GSGLMASNGIDAGIKLRPSAEDLEVLGP--EFQ--EHWQRVFANALDKPISW 394 FOMPI_156775 334 VRNDEEDI------EKWTAQWLKD-GTGLMAHNGIDSGIKLRPSDEELKIIGP--EFT--EHWQHVFANAPDKPVLF 399 DICSQ_160139 330 ITNTT------FQTEQLAQWEAT-QTGDFTL-GACNQWAWERLPSNDTIF-K--SVSDPSS---G-PTAPHYQLIF 390 GANSP_124428 317 GLNTT------LQAEQLALWTNN-RTGDFTL-GACNQWAWQRLADDDPIF-MDSNVTDPSA---G-PTSPHFQLIF 379 DICSQ_171752 330 SLNTT------FQAEQLALWTNN-RTGDFTL-GACNQWAWQRLADDDPIF-K--NVSDPSA---G-PTSPHFQLIF 390 GANSP_67648 330 HRNAT------LASEQLQIWEAN-GTGLMGL-PPTNQFGWFRADPS--TF-KDLNATDPSA---G-PTSANFELII 390 DICSQ_102587 330 HRNAT------LSAEQLQIWEVN-GTGLIGL-PPTNQFAWLRVDPS--VF-QSLNATDPSA---G-TTSANFEMII 390 GANSP_67654 329 NRNAT------LAAEAFSLWETN-GTGPLSL-GGATQFGWLRIPEAETFF-RQLGVDDPSA---G-RTSAHFEHLP 391 GANSP_85135 327 RRNST------FAAQALALWQVN-GTGPLAV-GTGSQFGWLRVPQGIGFF-QSFGISDPSA---G-PTSAHFELLP 389 DICSQ_160546 329 HRNST------LAAEALALWKIN-GTGPLGL-GAASQFGWLRVPNATGFF-RSLGVEDPSA---G-ETSAHFEQIP 391 PHLBR_22550 316 IRNAT------FRNELLAQWNET-HGGRLGD-AYSTWTGFSRMPPNASIF-E--THPDPAA---G-PNSPHLQSNV 376 TRAVE_176148 332 NRDPT------LMNQLLALYDSK-KQGPVANNPGGNQIGWFRLPSNSSVL-K--QFGDPTA---G-PLSPHFELTF 393 GANSP_117498 330 NRNPD------LFNAALEIYNAT-KQGVIANNPGGNQIGWFRLPRNSSVL-E--QFGDASA---G-PSSPHFELTF 393 DICSQ_182736 332 NRNPD------IFNAAMAVYNAT-KQGVISNNPGGNQIGWFRLPPNSSVL-S--EFGDTTA---G-PLSPHFELTF 393 TRAVE_40237 332 SRNAT------FADALLQQWLSE-RQGVMTL-DGQTQIGWLRIPTNDSAF-D--GTPDPST---G-RLSPHYELFV 392 TRAVE_133945 333 SRNGT------FAGALVGEWLAA-KQGAMTV-GGQSQLGWLRIPANDSAF-K--GTSDPSA---G-PLSPHYEFLF 393 GANSP_130042 335 GRNAT------LLNDLLTEWETS-KQGPLGN-GPSNHVGWLRVPEDEQTW-A--AGEDPSA---G-PTSPHYELLF 395 GANSP_138009 335 VRNTT------LYNDLLTEWEMQ-KQGVMAN-GGSNHVGWLRIPQDDPVW-L--AETDPSA---G-PTSPHYEFLF 395 DICSQ_103879 335 VRNST------LFNELLQEWKDK-RLGTMTG-NGFNHIGWLRLPTDDPIW-K--TAQDPSA---G-PTAAQYEFLF 395 DICSQ_96414 335 GRNST------LFDELLQEWEEQ-RQGVMTN-GGTNHIGWLRLPADDPIW-E--TEEDPSA---G-PAAPHYEFLF 395 FOMPI_40728 333 TRDAT------LATELYAQWNAS-GTGPYAD-GSPPQLIWTRVSNESTFFND--TFPDPAS---G-PTSPHIEISV 394 RHOPL_55496 332 ARNAT------LAAEALVQWNAT-GTGPFVD-NGVEQIGWARVPES--IF-Q--QYGDPSA---G-QNSPNLEFII 390 GELSU_118493 332 ARNAT------VATELLQEWEET-GTGRYCD-PGTNQIAWLKVGAGLE------DASA---G-PTAAQIELMF 386 RHOPL_54008 324 ARNAT------LADELLQEWNTT-GTGVFVD-DGTNQIAWTRLANETEVF-K--NVTDPSA---G-PASPHIELIP 384 GELSU_137959 332 LRNST------LTLEELEQWETT-GTGRDVD-QGLNQLIWLRLPPDER------PSPDLSA---G-PFAPHFEMLP 389 GELSU_117387 332 ARNAT------LEEKDLEQWETT-GTGRFVD-AGANQIMFFRLPADEQ------PAEDVSA---G-PSAAQYEILP 389 GELSU_84544 333 ARNAT------LVADDLAQWEAN-GTGKFSD-PGANQIVWLRAPEQPP------PSLNAAA---G-PIAPQIEILP 390 PHACH_37188 336 SRDRA------VYDANMAQWLAN-RTGLFTA-TPGNTIAYCRIADEELAA-R--NMSDSAP---G-PNSPHYEMLF 396 PHACH_135972 330 YRDTS------VFNAKLAQWQSS-RTGLFAD-SPTNALGFLRIPDDAAIW-S--QFEDPAA---G-PNSGHFELLF 390 DICSQ_86071 335 RRNTT------LIEAAIAQWQTN-RTGRLVD-SPVQSLAFMRVPANSRIF-D--NVTDPSA---G-PHSGHFEFLF 395 BJEAD_156054 329 LRNQS------LFDIGLAQWQKN-HTGIFAN-CPGSALAFLRVPKNNSAV-K--EFGDPSA---G-AHTPHIEMIF 389 BJEAD_171059 328 LRNQT------RFNAFLEQWETS-RSGPFAD-SVGLAVAYLRLPDNSTIF-S--NFTDPAP---G-TTTPHFEMIW 388 BJEAD_114954 322 LRNKT------VFNTQLAEWRAT-RGGPFSD-GLGLGVAFLRIPSNSSVF-T--NFTDPAP---R-PISFGLSCRL 382 BJEAD_183896 328 LQNQT------LFDELMAQWQFN-RTGPFAY-TSGVALAFLRLPRNSSIF-A--NFPDPAP---H-ETTPHFEMLF 388 BJEAD_114902 327 VRDPN------VFAADLAQWQNN-HTGLFAN-SPANSVAFLRVPSSDPIF-T--MFKDPTP---AGNTTPHFEMIF 388 BJEAD_52991 329 IREPS------ILNANLAQWMTN-HTGLFAN-GPANAVGFLRIPNNSTIF-E--NFTDPAP---G--NTPHFEMIW 388 BJEAD_245297 330 ARNAS------LMDANLNEWMVN-RTGPLTD-TPATAVGFLRLPDNSSIF-E--KVPDPSA---G-PRAGHYEFVF 390 BJEAD_71431 330 LRDPE------VFERVLAEWQTN-RTGPLVD-TPATAVGFLRLPDNSSIF-Q--TVPDPSA---G-PGAGHYEL-- 388 BJEAD_171002 330 FRDRD------VSNTFLAQWKQN-RTGPMVA-SPSTGLGFFRLPDNDTIF-E--RFADPSP---G-EGAGHIELIL 390 BJEAD_245049 329 LRDPA------TFAAALEMWQTN-KTGPLVV-TPATALGFLRLPDDDPIF-Q--TVQDPTA---G-PGAGHIELIF 389 BJEAD_66377 335 FRDPA------VLGAKLEQWMTS-KTGLFSN-SPGNIQAYYRLDENDPLL-T--ENPEVAS---G-PNSPHFEAIF 395 PHACH_6199 343 LRNHA------AAAAAFARWTEQ-RQGLFVN-SPANTHSFVRLPDDKPPL-A--THPDPAA---G-PNSAHLEYVY 403 PHLBR_131358 329 LRDNN------VFNADLAQWMSN-RTGLFVD-SPGNTQSFFRIPDDDPIW-L--LYKDPAA---G-PNSAHMETIY 389 PHLBR_164178 325 LRNNT------VFDDYMAEWQAS-KQGLFVD-SPANVLAFMRLPSNASIF-E--EYPDPSP---D-QCSAHTELIF 385 RHOPL_108489 335 YGNYSNLNDVPTTSTPFLSFINSATAYVNFSALIAAPETYAQQIADAVDSSASTLV----PSQYSEV-VEG------YKAIYNLTAQKLLLS------SIGDMEILL 424 PHACH_131961 339 ----DETTDVLADPLMLVLVRALASEYCNIQSAQDGHSWKDQAHAQNTEVLANTLPSLKSGLEKQYA-IQRKLLNDKHQSQAEYVQLYNSIKVKLS------VSPPSRLLQF 439 RHOPL_128830 302 -----QKELYFANRTGLLAATDNTVTLIPLQSVVDDEEFASLLALFDHEAQKPGLS---QLQKLQYP-IQRSWLETGDGPYVELI------QW 379

TRAVE_174721 440 QP------SHPWHT------QIHRDAFSYGAVQ----QSIDSRLIVDWRFFGRTEPKEENKLWFSDKITDTYNMPQPTFDFRFPAGRTSKEAEDMMTDMCVMSAKIGGFLPGS 536 PHLBR_123747 407 SA------DHPWHT------QIHRDAFSYGAVA----ENIDPRLIVDFRFFGYVDPSESNKIVFQKYYSDAYDMPQPTFKFQMSSED-RKRARRMMDDMCKTALKIGGYLPGS 502 PHACH_137275 446 TE------EHPWHV------QIHRDAFSYGAVA----ENMDTRVIVDYRFFGYTEPQEANELVFQQHYRDAYDMPQPTFKFTMSQDD-RARARRMMDDMCNIALKIGGYLPGS 541 BJEAD_34622 433 TK------EHPWHT------QIHRDAFSYGAVA----ENIDTRLIVDFRFFGYAEPQECNRIVFQQHYTDAYGMPQPTFKFQLSEDD-RERCRRMMDDMCSIALEVGGFLPGS 528 GELSU_84792 592 ------N--TTLPYNASQIFTITVYLSSGITSRGRIGVTAG---LNAVALEDPWLTD--PVDKVVLIQAL------EDVISTLPSVPDLTMITP------666 TRAVE_73596 585 ------N--TSVAYNASEIFTITLYLSNGIQSRGRIGVDAA---LNAKALVNPWLTN--SVDKTVLLQAL------HDVTSTMKNVPGLTMITP------659 DICSQ_153749 586 ------N--TSLPYNASQIFTITVYLSQGIQSRGRVGITSG---LNAQAITNPWLTD--TEDKTILLQAL------HDVADNIKSIPNLTLITP------660 GANSP_86428 585 ------N--SSLPYNASNIFTITVYLSQGITSRGRIGIDAA---LNAKALSNPWLTD--PTDKTVLLQAL------HDVISNMDSISNLTLITP------659 PHLBR_160653 594 ------N--TSVPYNTTQLFTITLYLSTGIQSRGRIGIDAA---LRASPIQNPWLVE--PIDKVVLLQAL------NDVATNIKSIPNLTMITP------668 BJEAD_45135 595 ------N--SSLPYNASQIFTITVYLSTGIQSRGRVGIDAA---LRASPLVNPWLVD--PTDKKVLLQAL------NDVAASVNDVSGLTMITP------670 PHACH_11098 591 ------N--SSLPYNASQIFTITVYLSTGIQSRGRIGIDAA---LRGTVLTPPWLVN--PVDKTVLLQAL------HDVVSNIGSIPGLTMITP------665 GANSP_114505 400 GSIVGGA-YADHALLP--PGKYVTMFQYL-EYPASRGKIHIRAP-SPYVEPFFDSGFLNN--KADFAPIRWSY------KKTREVARRMDAFRGELASHHPRF 489 DICSQ_181599 400 GSIVAGA-YADHSLLP--PGKYVTMFQYL-EYPASRGKIHIKSA-NPYVEPFFDSGFMNN--KADFAPIRWSY------KKTREVARRMDAFRGELASHHPHF 489 TRAVE_144610 400 GSIVAGA-YADHALLP--PGKYVTMFQYL-EYPASRGKIHVKSA-NPYVEPFFDSGFMNN--KADFAPIRWSY------KVTREIARRMDAFRGELTSHHPHF 489 PHLBR_128980 396 GSIVSGA-YADHALLP--PGNYITMFQYL-EYPASRGKIHIRSS-NPYVEPFFDSGFLNN--KADFAPIRWSY------KKTREVARRMDAFRGELTSHHPHF 485 BJEAD_34705 400 GSIVAGA-YADHTLLP--PGNYITMFQYL-EYPASRGKIHIKSA-NPYVEPFFDSGFLNN--KADIAPIRWSY------KKTREVARRMDAYRGELTSHHPRF 489 PHACH_126879 407 GSIVAGA-YADHTLLP--PGKYITMFQYL-EYPASRGKIHIKSQ-NPYVEPFFDSGFMNN--KADFAPIRWSY------KKTREVARRMDAFRGELTSHHPRF 496 FOMPI_127556 399 GSIVGGA-YADHSLLP--PGKYITMFQYL-EYPASRGKIHVKSA-NPYVEPFFDSGFLNN--KADFAPIRWSY------KKTREIARRMDCFRGELTSHHPHF 488 GELSU_80773 400 GSIVAGA-YADHTLLP--PGKYITMFQYL-EYPASRGKIHVKSA-NPYVEPFFDSGFMNN--KADFAPIRWSY------KKTREVARRMDAYRGELTSHHPHF 489 RHOPL_118723 399 GSIVGGA-YADHSLLP--PGKYITMFQYL-EYPASRGKIHIKSA-NPYVEPFYDSGFLNN--KADFAPIRWSY------KKTREVARRMDCFRGELTSHHPHF 488 WOLCO_24953 399 ASIVGGA-YADHSLLP--PGKYITMFQYL-EYPASRGKIHIKAA-NPYAEPFFDSGFMNN--KADFAPIRWSY------KKTREVARRMDAFRGELTSHHPHF 488 TRAVE_43286 408 IGELAMY-VGPSQPSP--DVLCFCTGGFS-MYPSGIGSVHITSGEDPHAPVDFVSGVFSS--PDDLAVMRHMY------KRCREIGRRMSCFRGLYAPDHPTF 498 DICSQ_149587 399 IGMISQY-VGTAPPPP--DHKCYCTFAYL-TYPAGTGSVHITSADDVTAPVDFDSGVCSR--KEDFALMRHMY------KVCREYGRRMDSYWGEIAADHPKF 489 GANSP_116439 407 FGIISQY-IGTAPPPP--SHKCYSTFAYL-TYPAGTGSVHVTSADDVTAPVDFDSGVCSR--KEDVALMRHMY------KVCREYGRRMASYRGEIARDHPRF 497 DICSQ_173648 405 AGQISQY-VGLSPPAA--NHKAFCTGVYS-THPVGTGSVHITSAHDVHSPVDFDTGVLSR--KEDLAVMRYAW------KLAREYGRRMSSYRGEYAPDHPTF 495 GANSP_116436 405 TGEISTY-VGVRPPAA--NHKVYCTGAFS-LHPVGTGSVHITSA-EVGSPVDFDTGVFSR--NEDLAVMRHAW------KRGREYGRRMTSYRGEYAVDRPEF 494 PHLBR_157963 406 LGQGSML-IGVPPILP--PQNFFTLGYFN-LYPLARGSVHITSADDVSAPLDFKLGYLEN--VADVKLLVWAY------KYTREVARRMPSFRGEPSPVHPKF 496 PHLBR_157964 407 IGLGAML-IGDPTAVP--SQKYFTIGYFN-LYPLARGSVHITSK-DTDAPLDFKAGFLEN--IADVTPLIWGY------KYTREIARRMPSFRGEPAPLHPDF 496 PHLBR_128210 395 MGVISML-VGDILNAP--IGKYFSSSYYL-AYPSARGFVHITS-DDPTVLPHFETGYLKH--ADDVALLRWAY------KRSREISRRMPCYRGEYAPWHPRF 484 BJEAD_143000 402 HTALNVL-VGDPAG-V--QSKFFSMGTFT-CHPTGRGWIHIRDGQDPSVQPEFVSGFLST--PDDLALCKFAY------KRTREIARRMACFRGEHAAGHPQF 491 BJEAD_45314 401 HTALNVL-VGDPAG-V--QSQFYSMGTFV-THPTGRGWIHIRDGQDPSVQPEFVTGFLST--PDDMALCKFGY------KRTREIARRMACYRGEHAPGHPQF 490 PHACH_5574 400 FGPVSML-VGDPSACP--PRKYFSLGYYV-EHPSSLGFVHVRDGRDPAVPPAFETGFLRT--PDDFALLSWGY------KRSREYARRMRCYRGEYAPSHPQF 490 BJEAD_241975 402 IGPVSML-VGDPSTTP--NRKYFSVGYYV-EHPTSLGFVHIRDGSDPTVPPEFETGFLKT--ADDFALLKWGY------KRSREFARRMACYRGEYVPNHPQF 492 PHLBR_29466 407 FGPVSMF-VGDPTTSP--SRKYFSLGYYV-EHPTSVGFVHIRDGEDPSVPPEFETGYLKT--SDDFALLKWGY------KRARELARRMPCYRGEYVPNHPVF 497 RHOPL_129158 400 IACSAGF-VGNPMGVP--PRRYSTIAYAI-WYSSAVGYLHITDANDVDAPLDFDPITING--KEDIAAMRWAY------KHGRELARRMGLHRGEFDGGHPAY 490 RHOPL_55972 385 IACAAAF-LGNPVGMS--PRKYITIGYTV-WYSRAVGYLHATSADDVNSPLDFDPQTISG--PEDLALQRWAY------KQSRDIGRRMPLYRGENKGGHPAF 475 BJEAD_227734 403 MCPLSMF-IGDPLSVP--PRKYFCMGYFV-AYPSSRGSLHIKDGQDPSAPLDFDTAYCKN--DDDLAVLKFGY------KRTREFARRMACYEGEYTPAHPVF 493 PHLBR_27956 401 MCPLSMF-IGDPLSVL--PRKYFCMGFFP-GYPTSTGHVHIRSGEDIQAALDFESAYCKS--PEDMAVLRFGY------KLSREFARRLPCYEGEYTPKHPTF 491 PHACH_6010 386 FCPLSMF-IGDPTSVP--PRKYFCMGYFP-AYPKAMGHVHIRSG-DVSAALDFETAYCRA--PEDMAVLKFGY------KLTREFARRMPCYEGEYTANHPQF 475 DICSQ_157363 399 FGLLALY-LGDLSKVP--VDKAYSVGWYL-QHPASIGRLHITSRDNVEAPLDFHPGYLDR--PEDMAVHKWGY------KMSREFARRMPSYRGEVPGAHPAF 489 GANSP_130292 398 FGLVALY-LGDRSQVP--VNKSYAVGWYL-QHPTSIGYSHIMSHDDVEAPLDFHPGYLDS--PEDMAVHKWGY------KMSREFARRMPSYRGEVAGGHPAF 488 TRAVE_167157 400 IATLSVL-TGDRPASATIGGKYWSVGWYL-EHPTSIGFVHITSANDVEATSDFHPGFLDS--PEDMALHKWGY------KRTREYARRLPSYRGEVEGRHPAF 492 TRAVE_170473 404 MGTMSMF-LGDRSQSS--VKKGYSIGWYI-QYPMSLGHVHITSADDVQSPLDFHPGYLDS--PEEMTLHKWGY------KRSREFARRLPSYRGEVVASHPVF 494 RHOPL_106935 376 LGILAQY-VGDPSTVP--ARKYCCADYFL-DYPASIGYVHITST-DVDAPPDFDPKFLSC--PEDLALLRWGY------KHGREIIRRMPLYEGEYAPAHPAF 465 WOLCO_121505 404 LGILAMY-VGDHSTVP--DRKYFCMDYFL-DYPATVGYVHITSADDVNAPPDFDPNFLSS--PDDLALLRWGY------KHGRELSRRMPSYRGEYPPGHPVF 494 WOLCO_132654 404 LGPFAQY-AG-YAPNP--PNKCCNMGFFI-EYPQSRGSVHITSADDVQAPPDFDPQFLKH--KGDVALLRWAY------KRGREIARRMPSYRGEFAPDHPAF 493 FOMPI_90445 399 FGAVSEF-VGDPSTVP--NRKYASLGYYV-QYPLSTGYVHITSASDVSAVADFDPGFLLR--KEDLVLLRWGY------KHCREFARRMPLYCGEYLPKHPQF 489 WOLCO_25722 402 FGSLAMY-VGDPSTVP--PRKYYSIGYFL-EYPILSGYVHITSADDPYAAPDFDPKYLHD--AGDLALMRWGY------KRGREIARRMHLYRGEFLPSHPTF 492 FOMPI_129478 395 LGAVSLF-VGDHSIIP--AKKYYSMGYLI-LYPESIGHVHISSAEDVNAPQDFDPMYITK--RSDLAVLRWSY------KQSREIARRMACYRGECIQRHPVF 485 FOMPI_156775 400 LGPVSMF-VGDLSNVP--ARKYSSICYYL-EYPESIGYVHITSADDVTAPLDFDPKYMSK--PSDVAVMRWGY------KHGRELARRMASYRGEYADGHPAF 490 DICSQ_160139 391 SDLYIAFAG---GSRP--DGHFLTLISNL-YTPVSRGNITLRST-NAFDYPIINPGLLSDKGGFDIHAMTEAL------KAGRRFL-SAPAWKSWIVGEFGDS 479 GANSP_124428 380 SDLYIAFGGG--GAFP--SGHFLTLISNL-YTPMARGSLTLNTT-DPFTHPVINPGLLNDGAGFDIYTMRQAL------KAGRRSP-GAAAWKDWVVAEYGGS 469 DICSQ_171752 391 SDLFIAFSG---GAFP--SGHFLTLISNL-YTPTTRGSLTLNST-DPFTYPIINPGLLNDENGFDIYTMRQAL------KAGRRFL-AANAWKDWIISEFGES 479 GANSP_67648 391 SDNFASKRV---ALPA--EGRFLSMVTNV-VSPSSRGNISLASA-DPFDAPLVNPNLLGT--DVDLAIMRSAI------KAARAFV-AAPSWADYVISEFGAF 477 DICSQ_102587 391 SDNFASKRV---ALPA--EGRFLTFVTNV-VSPSSRGNISLASA-NPFDAPLINPNLLGT--DVDVAIMRSAI------KAARAFA-AAPAWSDYIISEFGAF 477 GANSP_67654 392 TNAFISTTE---ALPA--EGHFFTISVGV-MSPTARGNISLNSN-DPFDAPLINPALLEN--AVDLAIMREAI------KASRAFV-KAPAWSDYIVSEFGAF 478 GANSP_85135 390 VNGFVSKVL---PLPA--EGHFFSFITAL-ISPTARGNLTLNST-DPFDAPLINPNLLGT--PADLAIMREAV------KAARAFV-SAPTWSDYIIGEFGAF 476 DICSQ_160546 392 TNAFVSKTV---PLPA--EGHFFSIITAV-VSPTARGNVTLNST-NPFDAPLINPNLLGS--PVDVAIMREAV------KAARAFV-TAPTWSDYIVSEFGVF 478 PHLBR_22550 377 ENGAL------SPAP--TGHFISVGTNL-LTPTSRGSVTLNTS-NPFDPPLIDLACLTT--AFDVFGVREAI------KNARRLL-AAPAWAGYVLGAQGAL 459 TRAVE_176148 394 GNSFLSF-TQ--PVPD--TGNFMSMCVAL-VSPTSRGSVTLASA-SAFDAPLIDPAFMQT--ESDLAILTEAV------KAAHRFV-GAPAWKDFIIAPFADA 480 GANSP_117498 394 GNSFLSF-TQ--PPPS--TGSFMSMCIAL-VAPTSRGSVKLASA-SAFDAPLIDPAFMQT--RADVATLTEAV------KAAHRFA-AAPAWRDFVLAPFIDA 480 DICSQ_182736 394 GNSFLSF-TE--PVPA--TGNFMSMCVAL-VAPTSRGSVQLATT-SVFDAPLIDPAFMQT--PSDLATLTEAV------KAAHRFA-AASPWKDFIVTPFVDA 480 TRAVE_40237 393 VPGFMSS--SGTPTPA--AGFFNTFLTAL-VTPTSRGSFTLASD-DPFAAPVVDPNFLNT--PFDIAAMRFAV------RSAVRLA-SARAWRDFLTGPAAGF 480 TRAVE_133945 394 APGFVSS--GGTPAPT--TGFFNTFFTAL-VTPTSRGTVTLAST-DPFAAPIVDPNFLST--PFDIAAMRHAV------RSIVRFA-SAPAWSDFLTGPAADF 481 GANSP_130042 396 SPGFVST-IA--TTPA--TGNFMTVISVL-VSPSSSGSVTLASS-SPFDSPLINPAYLNT--TFDTQVMRAAI------RSAANFV-SADAWAGFVTGRAESF 482 GANSP_138009 396 RPGFTST-VPGTPVPP--TGYFMTISAVV-VSPSSTGSVTLSSG-SPFDPPIIDPAFLST--SFDVYAMRAAI------RSAARFV-SARTWDGFITGQASSF 484 DICSQ_103879 396 DDGYVA-----ATAPP--TGYFITVDTVL-VSPTSRGSITLASA-NPFDSPLIDPAFLNT--TLDIYVMRAAI------RSAAHFL-SAKTWDGFVTGQGGDF 480 DICSQ_96414 396 RPAFASS-IPGTPTPA--TGNFMTVLAVL-VSPTSTGSVTLSSA-SPFAPPVIDPAFLST--PFDVYTMRAAI------RSAARFVSSAQTWDGFVTGQGAGF 485 FOMPI_40728 395 ENGFISF-VQ--SLPA--TGNFISMVTIV-SSPASRGSVTLASS-DPFDFPLIDPGFFTD--PIDVDIMVEAI------RQSMEFL-RASAWTGYVLQEVSPF 481 RHOPL_55496 391 ANAWVSW-IQ--PRPA--TGNYLSVLTTV-VSPGSRGAVNLASN-SIWDMPLIDPGYFSD--PFDVAAMVHAI------NMSKTFV-QGPTWEGYVIDPVSEL 477 GELSU_118493 387 FDGFASL-VQ--AVPP--TGQYFTIGAAV-SSPFSRGSITLAST-DPFDFPLIDPGLLTD--SRDMSVMIQAV------RIGMKMV-EASAWNGYILGPATEL 473 RHOPL_54008 385 ADGYASF-VA--PLPE--TGNYLTILTNV-VSPASRGSVTLASD-DPFDDPLIDPGFYTN--PLDLYSMVEAI------KQARRFL-SASAWDGYVVSETSYL 471 GELSU_137959 390 ADGFGSF-IQ--PIPP--TGFFFTMFTAV-VSPVSRGSVTLASN-NPFDFPLIDPGLLSD--PTDVSIMVNAI------KTAARFL-QSPAWAGFIIEPTASL 476 GELSU_117387 390 VDGFVSF-IE--ATPD--TGNFLTLATAV-VSPASRGSVTLANN-DPFAKPIIDPGLLSD--PTDVDVMMQAV------KASLQFV-TAPAWEGFILSPAADL 476 GELSU_84544 391 VDGFVSF-VE--ATPD--TGFFLTLASIV-VSPLSRGSITLASA-DPFTSPLIDPGLLSS--PTDVSIMVDAI------KASLQLL-TASAWDGFVISPTADL 477 PHACH_37188 397 ADGWSAS-LN--SIPS--TGHYFTINTIV-VSPTSVGSITLRSP-DPFTPPRIDPAFLAT--EFDAFAMLAAV------RAARQYV-RTTPWAGFIVAPYGAV 483 PHACH_135972 391 LDGYAPA-----PAPG--PGNYFTINSAV-VTPESVGSVKLNSS-SPFAFPLIDPGFFTA--PFDIQAMVYAV------KAARAFM-ESAPWAGVALSRIGAV 475 DICSQ_86071 396 ADGYGAV-SV--PQPP--TGHFLTVFSAV-VAPTSVGSIKLASS-DPFDNPLIDPGFFST--EFDTLVMLEAI------KTARQFM-ALPAWGGLVSGRFGPV 482 BJEAD_156054 390 ANGYTTT-VD--PQPA--TGHFLSINTVA-VTPLSVGSIQLASA-DPFTFPLINPNLLDS--PFDQALMIESV------RTARRFV-QTKPWQGFIVSPIGRL 476 BJEAD_171059 389 ADGFASNEVA--QAPP--TGHFMTINTAI-MTPLSLGNLTLASA-DPFTFPTINPNFFES--QFDQFIMVEAV------KAARRFV-QAPAWDDYVVDRFGLI 476 BJEAD_114954 383 QDGLALSDVA--TTPP--TGNYLTVNVAV-MTPFSLGSVRLASS-DPFTFPLIDPNYFAS--PFDRLAIVEVV------KLARQFV-QTPAWDGFVMDRFGTV 470 BJEAD_183896 389 GDRFAATDVA--SRPP--TGNFITINTAV-VTPLSLGSVRLASA-DPFTFPLIDPNYFAS--PFDRFAMVEAV------KAARRFL-NAPAWKDLVVGRFGTV 476 BJEAD_114902 389 ADGFGQT-VL--SEPA--NGSYLTINTAV-VAPLSVGSLRLASS-DPFEFPLIDPNFFAS--PFDRHIMLQAV------KAARRFV-QTPAWNGFVTGLFGPV 475 BJEAD_52991 389 ADGFAQT-VL--TQPA--EGHFMTINTAV-VAPLSTGSVRLAST-DPFTFPLIDPNFFAS--PFDQAVMVESV------LAVRRFV-DTQPWADFILGRFGPV 475 BJEAD_245297 391 TNGFAPD-VA--TPPA--TGNFLTLHTAI-MSPTSVGSIKLNTS-NPFDFPLMDPNFCST--EFDMFTMLYAV------KAARRFL-QAAPWEGFITDRFGVA 477 BJEAD_71431 389 -NGFAPD-VA--SIPE--TGHFVTVHSAI-MTPTSTGSIRLASS-DPFEFPLIDPNFFST--EFDQFTMVEAV------KATRRFM-DAAPWQGFATSRTGTM 474 BJEAD_171002 391 SNQWTPE-VQ--IPPA--TGSFLTVHAVV-VSPASEGTVRLNST-NPFEFPLLDPQLLSS--EFDQHTIIHAA------RLARQFV-SSSPWADFVESRTGII 477 BJEAD_245049 390 SNDFVPQ-VQ--LVPP--TGNYMTICTIV-TSPTSTGSIHLNST-DPFAFPVMDPGFLAT--DFDMYVMKQAV------HAARAFV-QAGPWADFVTGRLGFV 476 BJEAD_66377 396 VSGFAPFGPI--PPPS--DGNWLTILVGL-MATTSRGHVNVSSP-DPFASPLIDPQILST--SFDKQALRAGA------RMILDFV-RADAFKDYILEPHDTL 483 PHACH_6199 404 CNGYAPFGGA--PPPA--EGNYMTILTGL-VSPVSRGTVCIRSP-DAFAAPQIDPALLTH--PFDASAMIHAV------ARAFALT-RDTVLARYVVRPVGTA 491 PHLBR_131358 390 VNAFAPFGTT--APPT--SGNYLTLLAAL-VAPMSRGTVLINSS-DPFEHPLIDPSLLTS--IFDVYAMVQSI------KGAQEFI-KAPVFKEYVGSAYGDL 477 PHLBR_164178 386 VDSFAPFGAV--AQPV--EGYFITILVAV-VSPLSRGTVKLASS-DPYNKPLIDPAFLTH--DFDTYAMVQAL------NDVDEIV-ASSAWAGYVTGPYGDL 473 RHOPL_108489 425 S------LTG--AGQY--EPQVISIQAAL-QHPFSQGRLYINSS-DIFEYPIIDPQYLSN--NADLVMLREGL------RFCRSLGNTAPLSAAMAEEISP-- 505 PHACH_131961 440 AGRQPMPYAP--PAEP--GKRYTSLFCAL-THPLSRGTVHLASA-DPLAPPAIDPNYFAN--AADLRLVVRTV------QYALKLYATPP-LADHVTRTLLPP 487 RHOPL_128830 380 SE-----GFY--DPQP--NESYIVILGGN-MHPTSRGSVHIDSS-DPFVHPVINPQFLSH--EFDVAVLLNVL------KFVQRIGNLEP-FATMIAAQTD-- 500

TRAVE_174721 537 L------P------QFMEPGLVLHLGGTHRMGFDEQ----EDKCCVNTDSRVFGFKNLFLGGCGNIPTAYGA 592 PHLBR_123747 503 E------P------QFMTPGLALHLAGTVRAGK------ETVADTYCKVWNFDNLYVGGNGVIPTGFAA 553 PHACH_137275 542 E------P------QFMTPGLALHLAGTTRCGLDTQ------KTVGNTHCKVHNFNNLYVGGNGVIETGFAA 595 BJEAD_34622 529 E------P------QFMTPGLPLHLAGTTRAGLDQK------TTVADTYSKVWHFSNLYVGGNGVISTGFGA 582 GELSU_84792 667 ------DSGMTLEEYVDLY-DPSTMCSNHWVGSAKMGTSSD------TAVVDENAKVFNTDNLFIIDASIVPSLPVG 730 TRAVE_73596 660 ------DNTMTLEQYVAAY-DPATMCSNHWVGAAKMGTSSS------TAVVDENAKVFNTDNLFIVDASIIPSLPIG 723 DICSQ_153749 661 ------DPTMTLEQYVDAY-DPSTMCSNHWVGSAKIGTSAS------NAVVDQNAKVFNTNNLFVVDASIIPALPIG 724 GANSP_86428 660 ------DATMTLEEYVDAY-DPGTMCSNHWVGSAKIGTSSS------TAVIDENAKVFNTDNLFIVDASIIPSLPMG 723 PHLBR_160653 669 ------DYTQTIEEYVDAY-DPGTMDSNHWIGSASIGNSSS------NSVVDENVKVWGTNNLFIIDASIIPSLPTG 732 BJEAD_45135 671 ------DRTMTIEQYVDAY-DPATMNSNHWVSTATIGQNAT------TAVVDENTKVFNTNNLFVVDASIIPHLPVG 734 PHACH_11098 666 ------DVTQTLEEYVDAY-DPATMNSNHWVSSTTIGSSPQ------SAVVDSNVKVFGTNNLFIVDAGIIPHLPTG 729 GANSP_114505 490 HPDSPAAARDIDLQTARELLPNGLTVGIHMGTWHTPSEPYDASKVHDDIKYTAEDDQAIDDWIADHVETTWHSLGTCAMKPREQ------GGVVDKRLNVYGTQNLKCVDLSICPDNLGT 603 DICSQ_181599 490 HPDSSAACRDIDIKTAREILPNSLTVGIHMGTWHRPSEPFDASKVHEDIKYTEEDDKAIDDWIADHVETTWHSLGTCAMKPREQ------GGVVDKRLNVYGTENLKCVDLSICPDNLGT 603 TRAVE_144610 490 HPASPAACRDIDIKTAREIYPDGLTVGIHMGTWHRASEPFDASKVHEDIKYTEEDDKAIDDWIADHVETTWHSLGTCAMKPREQ------GGVVDKRLSVYGTENLKCVDLSICPDNLGT 603 PHLBR_128980 486 HPASPAAVRDIDIETAKQLLPDGLTVGIHMGTWHRPGEPYNASKVHENIKYTKEDDQAIDDWIADHVETTWHSLGTCAMKPREQ------GGVVDKRLSVYGTTNLKCVDLSICPDNLGT 599 BJEAD_34705 490 HPASPAVAKDIDIETAKQIYPNGLTVGIHMGTWHTASEPYDAGKIHEDIKYTAEDDQAIDDWVADHVETTWHSLGTCAMKPREQ------GGVVDKRLNVYGTQNLKCVDLSICPDNLGT 603 PHACH_126879 497 HPASPAACKDIDIETAKQIYPDGLTVGIHMGSWHQPSEPYKHDKVIEDIPYTEEDDKAIDDWVADHVETTWHSLGTCAMKPREQ------GGVVDKRLNVYGTQNLKCVDLSICPDNLGT 610 FOMPI_127556 489 HPNSPAACKDIDLRTAKELLPDSLTVGIHMGTWHKPGDAYDAKKVHDDIVYSKEDDEAIDNWVADHVETTWHSLGTCAMKPREQ------GGVVDKRLSVYGTENLKCVDLSICPDNLGT 602 GELSU_80773 490 HPASPAACKDIDIQTAKGILPNGLTVGIHMGTWHKPGDVYDPSKVHEDIQYTKEDDEAIDNWVADHVETTWHSLGTCAMKPREQ------GGVVDKRLSVYGTENLKCVDLSICPDNLGT 603 RHOPL_118723 489 HPNSPAACHDIDLKTAKELLPNSLTVGIHMGTWHKPGDAYDPKKVHDDIVYTKEDDEAIDNWVADHVETTWHSLGTCAMKPREQ------GGVVDKRLSVYGTENLKCVDLSICPDNLGT 602 WOLCO_24953 489 HPNSPAACRDIDIKTAKELLPNGLTVGIHMGTWHKPGDAYDPKKVHEDIVYSKEDDEAIDNWVSDHVETTWHSLGTCAMKPREQ------GGVVDKRLNVYGTENLKCVDLSICPDNLGT 602 TRAVE_43286 499 PDGSAAHGPEA------LTGMPVDVHAPDLVYTEEDDKAIDVILKSHILTAWHSNGTCTMKPRDQ------GGVVDPRLNVYGAQGLKVADVSICPANVST 587 DICSQ_149587 490 AASSAAAVPKA------P--VRPVSIDAVDIVYSAEDERALDDFIRSKVQTAWHSLGTCAMKPHDK------GGVVDSRLNVYGVQGLKIADMSIAPSNVAT 577 GANSP_116439 498 APGSTAAVIQG------PEQAASVGTDTPDIVYSAEDDRAIETHIKANVQTAWHSLGTCAMKPREK------GGVVDSRLNVYGVRGLKVADMSIAPSNVGA 587 DICSQ_173648 496 PQDSEAAVSQ------DHNTPVAIDAPDIVYTKEDDEAIDDFLKARIITAWHSLGTCAMKPRDQ------FGVVNSRLTVYGVQNLKVADMSICPSNVAT 583 GANSP_116436 495 PAGSAAAVRP------EHSTPVSIDAPDIVYTEEDDEAINDYLKANIMTAWHSLGTCAMKPRDK------GGVVDSRLNVYGVQGLKVADMSICPSNVAT 582 PHLBR_157963 497 PDGSKASAIQE------GTPFAVDHPRLEYSAEDDKAIEQHIRESVGTSWHSLGTCAMKARTE------KGVVDSKLNVYGTKSLKVADMSICPSNVGG 583 PHLBR_157964 497 PPGSKAGLIAE------GVPFTIDYPRVEYTKEDDEAIDRYVRKLAATCWHSLGTCAMKPRAE------KGVVDSKLNVYGTKSLKVVDMSICPSNVGA 583 PHLBR_128210 485 PEGSAAAAGLD------VRPVAVDAPDLLYTAEDDKAIEQHMKEYLNTTWHALGTCAMKPREQ------GGVVDPRLNVYGVEGLKVADCSIPPSNVGT 571 BJEAD_143000 492 SEKSDAACKAA------SGPVPINAPDIRYSEEDDKVLVEYLKVAVNTTWHSLGTCAMKPRDQ------GGVVDSKLNVYGVDGLKVCDLSVAPGNVAA 578 BJEAD_45314 491 S--EQAACKAA------SGPVPLEAPDIEYTEEDEKALTEYLKVAINTTWHSLGTCAMKPREQ------GGVVDSKLNVYGVEGLKVCDLSIAPGNVSA 575 PHACH_5574 491 APASAARTG-E------SAPVPFDAPDIAYTEEDEKAIEEYTRKFVQTAWHSVSFDVFGQ------HLSIPPSNVAA 554 BJEAD_241975 493 AAGSKALCKGE------IQPAAVDAPDLVYTAEDEKEIEEYTRKFVATAWHSLGTCAMKPREN------GGVVDPRLNVYGVEGLKVADLSVPPANVAA 579 PHLBR_29466 498 PAGSAALCKGE------ISPAAFDAPDIEYTAEDEKAIEAYTRKYVATAWHSLGTCAMKPREQ------GGVVDHRLNVYGIEGLKVADLSIPPSNVAA 584 RHOPL_129158 491 PEGSAAAVRPS------AEPTPLDTPKLVYTAEDDAAIDLFNRQQVGTMWHSLGTCAMKPRAQ------GGVVDSKLNVYGVTGLKVADMSIAPSNVGA 577 RHOPL_55972 476 ASDSPAAVQVE------GQPIKLSDPVVQYSAADDMAIDAFHKEVVATMWHSLGTCAMKPREQ------GGVVDSALNVYGVKGLKVADMSIAPSNVGS 562 BJEAD_227734 494 DESSPARCTGDVDAKG------EQVTVKPVPVSAPRLAYSAEEDQTLDEYVRRAVVTAWHFIGTCAMKPREE------GGVLDPSLNVYGVQGLKVCDLSILPSNVCA 589 PHLBR_27956 492 NETSEALCRQD------LKSVSIDAPRLTYTQEDDVALDVYLRKAVVTGWHFMGTCAMKPRKS------GGVVDANLNVYGVEGLKVCDLSIPPSNVCA 578 PHACH_6010 476 AEDSPARCREG------IAPVPVDAPRLVYSAADDEALEAYLRK-----FGGMGTCAMKPREQ------GGVVDHNLNVWGTEGLKVCV-TVAAANVCQ 556 DICSQ_157363 490 SETSSVAPRLH------DSPVPNATPSLVYTEEDEKALEDWIRKTVATAWHSLGTCAMKSREK------GGVVDSRLNVYDVDGLKVADMSICPGNVSA 576 GANSP_130292 489 SEKSQATPRLY------DGPVPISAPKFQYTDEDEKAIEDYIRQAVRTAWHSLGTCAMKSREK------GGVVDSRLNVYGVDGLKVADMSICPSNVAA 575 TRAVE_167157 493 HPTSKVAAGAR------DGPVPILSPDLEYSAEDDRALEDYIRQVVATTWHSIGTCAMKKRED------GGVVDSRLNVYGVENLKVADLSICPGNVAA 579 TRAVE_170473 495 APSSKAATGPR------SGPVPVTAPDIEYTAEDEVALEAFIRRVVATAWHSIGTCAMKKRED------GGVVDSKLNVYGVEGLKVTDASICPGNVGA 581 RHOPL_106935 466 SEESTAACRLQ------RAPAAIGDPPIVYTPQDDLAIDKFVRENAGTTWHSLGTCAMKAREG------AGVVDSRLNVYGVQGLKVADLSIAPANVSA 552 WOLCO_121505 495 PEGSLAACRAD------RRPVEINDPDIQYTAKDDKAIDEHIRKVVMTTWHSLGTCAMKQREA------GGVVDSHLNVYGVEGLKVADLSIAPSNVGA 581 WOLCO_132654 494 SEGSQAFCNME------ASPVDLSEPNIEYTPEDDYAIDTNNRKHLNTIWHSLGTCAMKARKD------DGVVDSSLSVYGVTNLKVADMSIAPSNVSS 580 FOMPI_90445 490 PEGSQASCSAD------AVPVPMDAAPLQYTAEDDKAIDQYSRSAVQTSWHSLGTCAMKPRDQ------GGVVDARLNVYGVTGLKVADMSIAPGNVCA 576 WOLCO_25722 493 PEGSKAKCHDN------VRPVPVSAPEIEYTAEDNEAIDNYTRKMLGTTWHSLGTCAMKARDK------GGVVDSNLNVYGVTSLKVADMSIAPSNVSA 579 FOMPI_129478 486 PKGSSAACKED------AKPVDMHAPDIQYTEEDDKAIDEYTRKTVATSWHSLGTCAMKPRDQ------AGVVDSSLNVYGVSGLKVADMSIAPSNVGS 572 FOMPI_156775 491 PAGSQAVCSAS------AGPTAIQEVDIQYTENDDRAIDEYTRRIVGTAWHNLGTCAMKPRDK------QGVVDSALNVYGVSGLKVADMSIAPSNIGS 577 DICSQ_160139 480 A------SA--QTDAEIESFIRQNALVVNHVSGTVGMGKANT--IAKGSGALNPDLTVKGVIGLRVVDASAFPFIPAA 547 GANSP_124428 470 A------NA--TSDAAIDAYIRKNALVVNHVSGTVAMGRSGGGGAGKGAGALNPDLSVKGVKGLRVVDASVFPYIPAS 539 DICSQ_171752 480 A------NA--TSDADIDAYIRKNALVVNHVSGTVAMGKSGD--SSKGAGALNPDLSVKGVKGLRVVDASAFPHIPAA 547 GANSP_67648 478 A------NA--TTDEELNAYIRDNADTVDHPIGTVPMGKGPG------GALNADLTVRGTVGLRVVDASAFPFIPSG 540 DICSQ_102587 478 A------NA--TTDEELDAYIRDNADTVDHPVGTVPMGKGCE------GALNADLTVKGTAGLRVIDASAFPFVPSG 540 GANSP_67654 479 A------NA--TTDEALEAYIRANADTFDHPAGSVAMGKGDE------APLTPDLKVRGTLGLRVVDASAFPFIPSG 541 GANSP_85135 477 A------NA--TTDSALDAYIRNTSDTIDHPVGTVAMGNDAD------RALDSHLKVKGTAGLRVVDASAFPFIPSG 539 DICSQ_160546 479 A------NA--TTDDELEVYIRKNSDTVDHPVGTVAMGQGTN------APLDSQLRVKGTVGLRVVDASAFPFIPSG 541 PHLBR_22550 460 A------N--ATTDAQIEAFARANAAPDGHVVGTAAMSAADA-----GFGVVDPDLRVKGVEGLRVVDASVFPFIPSG 524 TRAVE_176148 481 A------Q--TTTDDDIRAYIRNMIATFRHPMGTATMSAEHD-----AAGVVNPDLRVKKISGLRIVDASVFPHIFGA 542 GANSP_117498 481 T------N--TTTDADIHAYIANQVATFRHPMGTARMSTAEL-----GAGVVDSHLLVENVVGLRIVDASIFPHIFGA 545 DICSQ_182736 481 V------N--TTTDVDIHAYITNLVTTFRHPMGTARMSTSEF-----GSGVVNSNLLVNGASGLRIVDASIFPHIFGA 545 TRAVE_40237 481 A------EVDIHDDDAVDAWARTQASTIFHPVGTARMGKCGD---A-HGAVVNPDLTVKGARGLRIVDASILPLIPAA 548 TRAVE_133945 482 A------DVDIDDDDSVDAWARAQASTIWHPVGTARMGKCGD-----AGAVVNPDLTVQGARGLRIVDASVLPLIPAA 548 GANSP_130042 483 A------DVDLGSDSEVDAWVRSQGTTIWHPTGTAKMGRCGD-----KNSVVDPDLRVKGTRGLRVVDASVLPFIPAA 549 GANSP_138009 485 A------DVDLSSNDAVDAWARSQASTIWHPTGTARMGGCGD-----EDSVVDPDLRVKGTKGLRVVDASVLPFIPAA 541 DICSQ_103879 481 A------NVDLDLDESVDAWARARAMTVWHPTGTAQMGKCND-----TGSVVDPDLRVKGTKGLRVVDASVFPYIPAS 547 DICSQ_96414 486 A------DVDLNDDAAVDKWARAQASTIWHPTGTARMGGCGY---VEEGSVVDPDLRVRGTEGLRVVDASVFPFIPAA 554 FOMPI_40728 482 A------Q--ASTDAELAAYARNYSATENHPVGTARMSSASS-----SEGVLTPDLRVKGTSGLRVVDASVLPFIPAA 546 RHOPL_55496 478 A------A--AQTYEELEAYARNQVTTIWHPVGTARMPANSS---D-LEAVVTSELLVKGASGLRVVDASVLPYIPAA 543 GELSU_118493 474 T------P--ASSDAEIANFARNFTSTEFHPAGSARMGPESG-----QEGVVTPDLKVKGAVGLRIVDASVFPYIPAS 538 RHOPL_54008 472 S------A--AQTDEQIAEYARNFTSTVFHPIGTARMAANDS-----NEGVVTPSLLVKGTDGLRIVDASVLPYIPAA 536 GELSU_137959 477 A------T--AIADGNFEDYARNFTSTEFHPVGTARMTRASS-----KTGVVNSNLQVKNTSGLRVVDASVFPFILAG 541 GELSU_117387 477 S------EA-TASDTALEAYVRNSTSTVFHPVGSARMASESS-----SDGVVTPSLLVRNVSGLRVVDASIFPFIPAG 542 GELSU_84544 478 A------G--AKTDAALAAYARNSTSTVFHPVGSARMGPENA---A-SGSVLTPSLLVKGVSGLRVVDASVFPFIPAG 543 PHACH_37188 484 G------TA--ESDAEIVAAVRRNTVTIWHPTRTARMAPAHA-----AWGVVDPRLRVKGVHGLRVVDASVIPVIPAG 548 PHACH_135972 476 G------EA--ESDEDIIAAIRANTRTIYHPTSSARMSPADA-----SWGVVDPELRVKGANGLRVVDASAFPSIPAV 540 DICSQ_86071 483 G------DA--ETDEEIIAAARVSVETIYHPVSTARMSPRDA-----SWGVVDPNLLVKGASGLRVVDASVFPTIPAA 547 BJEAD_156054 477 G------AA--QTDAEILNELRDLVVTVWHPTSTARMSPRNA-----TWGVVDPQLRVKGASGLRVVDASVMPFVPAA 541 BJEAD_171059 477 G------SA--QTDADILEAARKAIVTIWHPSCTARMSPTNA-----TWGVLDPQLRVKGTSGLRVVDASSFPVIPAG 541 BJEAD_114954 471 G------SA--NTDDEIIEAARKSIVTIWHPVSSARMSPARA-----KWGVLDPKLRVKGTSGLRVVDASAFPIAPAA 535 BJEAD_183896 477 G------AA--ETDEEIVEAARNAVVTIWHPVSTARMSPIGA-----PWGVLDPDLRVKNTEGLRVVDASAFPVIPAG 541 BJEAD_114902 476 S------SA--SSDADIIAASKDAVVTIWHPTSTAKMTSARS-----KSGVVDPSLLVKGVSGLRIVDASVFPVIPAA 540 BJEAD_52991 476 G------LS--NTTDDIIAASRDAIVSIWHPTSTARMSPKNA-----TWGVVDNELRLKGASGVRVVDASIFPVIPAG 540 BJEAD_245297 478 G------AA--NTDEEIMEAIRAGVVTIWHPTSTARMSPKGA-----NFGVVDPDLRLKKVDGVRVVDASVIPEIPAS 542 BJEAD_71431 475 G------MA--ETDDEILAAARAGVVTIWHPTSTARMSPKQA-----DWGVVDPDLLVKGVSGLRVVDASIFPEIPAV 539 BJEAD_171002 478 G------DT-DDDEADLLAAARQATVTIWHPMCTARMAPKGS-----TDGAIDPDLLVKGVSGLRVIDGAALPAIPAT 543 BJEAD_245049 477 G------DA--STEDEIATAARQATVSIWHPVGTTRMSPKGA-----DYGVLDPDLRVKGISGLRVVDGSALPEIPAV 541 BJEAD_66377 484 R------GVWWDPE------DNDDDAEARQAAYITKTAWPFNHPAGSCAMRAN------GGAVDARFRVKGARGLRVVDSSVFPAQIHC 554 PHACH_6199 492 A------ELDSSDAPRVERFVRENAVTINHPCGTCAMGAR------EGVVDAKLRVRGVSGVRVVDAAVFPTIPNC 555 PHLBR_131358 478 A------HA--TTDLQLAVFAAENAVTVNHPSGTCKMSSANS-----NDGVVDSQLRVKGVSGLRVVDASVFPSVPNC 542 PHLBR_164178 474 A------NA--TTDAGKLAFARANSFNVNHCVGTAYMSPANA-----SAGVVNPDLTVKGTSKLRVVDASVFPVIPPN 538 RHOPL_108489 506 ------GSSVQSDEQWETWLAQNSYTEYHPSCSCAMLPQSQ------GGVVDANLRVYGLANVRVADASVFPFQFSA 570 PHACH_131961 488 P------ETLARGEAGIAEYVKAHCGPVFHPVGTAAMMPRAD------GGVVDPALKVYGTSNLRVVDCSIVPLELSC 593 RHOPL_128830 501 ------PLTNLTNGDLESYVRNSAAGGDHLIGTAAMAPRDA------GGVVDSSLVVYGTANLRVVDASVIPLLIAC 565

TRAVE_174721 593 NPTLTAMSLAIKSCEYIKNNFTPSPFTDQAQ*------624 PHLBR_123747 554 NPTLTSICYALRGASKIIEKLNGNL*------579 PHACH_137275 596 NPTLTSICYAIRASNDIIAKFGRHRG------621 BJEAD_34622 583 NPTLTSLCFAIRAADSIIAKLKCHK*------608 GELSU_84792 731 NPHGTVMSAAEQAVANILALSGGP*------755 TRAVE_73596 724 NPQGVLMSAAEQAVSRILALAGGP*------748 DICSQ_153749 725 NPHGQLMSAAEQAAAKILALAGGP*------749 GANSP_86428 724 NPHGMLMSAAEQAVAKILALSGGP*------748 PHLBR_160653 733 NPHGLLMSAAEQAVARVLALAGGP*------757 BJEAD_45135 735 NPHGLLMSVAEQAAARIIALAGGP*------759 PHACH_11098 730 NPQGTLMSAAEQAAAKILALAGGP------753 GANSP_114505 604 NTYSSALLVGEKGADLIAEDLGLKLRFPHAPVPHAPVPVGKPATQLVR*------652 DICSQ_181599 604 NTYSSALLVGEKGADLIAEDLGLKLRLPHAPVPHAPIPTGKPASPQARR*------653 TRAVE_144610 604 NTYSSALLVGEKGADLIAEELGLKIRVPHAPVPHAPVPTGKPAAPLFR*------652 PHLBR_128980 600 NTYSSALLVGEKGADLIAEELGLKVRTPHAPVPHAPVPAGRPSTQQPR*------648 BJEAD_34705 604 NTYSSALLVGEKGASLIAEELGLKIRTPHAPVPHAPIPAGVPATQQPRHY*------654 PHACH_126879 611 NTYSSALLVGEKGADLIAEELGLKIKTPHAPVPHAPVPTGRPATQQVR*------659 FOMPI_127556 603 NTYSSALLVGEKGASLIAEELGLKLRFPHAPVPHAPIPAGKPATQVR*------650 GELSU_80773 604 NTYSSALLVGEKGADLIAEELGLKIRVPHAPVPHAPVPTGKPGTQQVR*------652 RHOPL_118723 603 NTYSSALLVGEKGADLIAEELGLKIRVPHAPVPHAPIPTGKPATQQLR*------651 WOLCO_24953 603 NTYSSALLVGEKGADLIAEELGLKIRHPHAPVPHAPIPVGKPATQLAR*------651 TRAVE_43286 588 NTYSTALTIGEKAAVLIAEELGITGV*------614 DICSQ_149587 578 NTYSTAITIGEKAAMIIAEDLGIKGV*------604 GANSP_116439 588 NTYSTAITIGEKAAVIIAEELGVQGV*------614 DICSQ_173648 584 NTYSTAVMIGEKAAAIIAEDLGLAEV*------610 GANSP_116436 583 NTYSTAVMVGEKAAVIIGEDLGIANV*------609 PHLBR_157963 584 NTYSTALVIGEKAAVIIGEELGITGV*------610 PHLBR_157964 584 NTYSTALVVGEKAAVIIADELGVPGV*------610 PHLBR_128210 572 NTYSTTLAIAEKAAVLIAEDLGIQV*------597 BJEAD_143000 579 NTYSTALLIAERAAVLFAEELGITGV*------605 BJEAD_45314 576 NTYSTALLIAERAAVLFAEELGVVGV*------602 PHACH_5574 555 NTYSTTLAVAEKAALIIAEELGIQGV*------581 BJEAD_241975 580 NTYSTTLAVAEKAALLIAEDLGVKGV*------606 PHLBR_29466 585 NTYSSTLAIAERAAVIIAEDLGVKGV*------611 RHOPL_129158 578 NTYSTALTIGEKAAAIIGEELGLTI*------603 RHOPL_55972 563 NTYSSAVLIGEKAATIIASELGVKI*------588 BJEAD_227734 590 NTYSTALVVGEKAAVIIGKELGVNVTPA*------618 PHLBR_27956 579 NTYSTSLVIGEKGAVIIANELGIDGI*------605 PHACH_6010 557 NTYSTALLVGEKGAVIIGNELGIAV*------582 DICSQ_157363 577 NTYSTAILIGEKAAVLIGQELGIHIDDGLTPLARL*------612 GANSP_130292 576 NTYSTAIVIGEKAAVIIAEELGIPLNDGSASKFLGKYPASKL*------618 TRAVE_167157 580 NTYSTAVLIGEKAAVIVAQELGISMEDAGPLAMRARL*------617 TRAVE_170473 582 NVYSTAVLIGEKAAMIIAEELGITLQDD*------610 RHOPL_106935 553 NTYSTAVLIGEKAANLIAEELNICTV*------579 WOLCO_121505 582 NTYSTALVIGEKAAQIIANELGVDDF*------608 WOLCO_132654 581 NTYSVALVIAEKAASLIMQELGI*------604 FOMPI_90445 577 NTYATALVIGEKAATIIMLELGIFGDHT*------605 WOLCO_25722 580 NTYSTALAIGEKAASIILHELGRRNSGCENPRQRCLL*------617 FOMPI_129478 573 NTYSSAVVIGEKAATIILAELVKQ*------597 FOMPI_156775 578 NAYSTAVVIGEKATTIILKELASQ*------602 DICSQ_160139 548 HTQVPTYALAERAADLIKACD*------569 GANSP_124428 540 HTMVPTYILAERASDLIK*------558 DICSQ_171752 548 HTMVPTYILAERAADLIKNSTSTASGSKSGSGNGAFTPSAT------LVSVFAPLVALVALLL*------605 GANSP_67648 541 HTQGPTYILAERAAALVKATISRGHGSRGIN*------572 DICSQ_102587 541 HTQGPTYILAERAAELVKASLGQQERSRHHRGGNKY*------577 GANSP_67654 542 HTQGPTYILAERAADLVRADLRT*------565 GANSP_85135 540 HTQGPTYILAERAAHLVRTDW*------561 DICSQ_160546 542 HTQGPTYILAERAAHLVRSSL*------563 PHLBR_22550 525 YTMFPTYMIAERGSDLIKASWQ*------547 TRAVE_176148 543 HLQAPVYAIAERAADLIKQAHGIPLSR*------573 GANSP_117498 546 HLQAPVYAIAERASDLVKRAHGIPL*------571 DICSQ_182736 546 HLQAPVYAIAERASDLIKKEHGVPLLS*------573 TRAVE_40237 549 HPQAAIYVFAERVADLIKNGTGRCE*------574 TRAVE_133945 549 HPQAAIYAFAERVADLIKNGTSSCE*------574 GANSP_130042 550 HPQAVVYAFAERAANLIKLGTHAC*------574 GANSP_138009 542 HPQAVVYAFAERAADLIRNAGNM*------564 DICSQ_103879 548 HPQAVVYAFAERAADLIKDGRRYC*------571 DICSQ_96414 555 HPQAAVYAFAERAADLIKSGRRFC*------579 FOMPI_40728 547 HTQASVYAVAERAADIIIESWA------568 RHOPL_55496 544 HTQAPVYALAERAADLIKVAWRV*------567 GELSU_118493 539 HLQACVYATAERAADLIKSDWL------560 RHOPL_54008 537 HPQACIYAVAERAADLIKAAWGESM*------562 GELSU_137959 542 HPQAGIYALAERAADLIKADWL------563 GELSU_117387 543 HPQAAVYAIAERAADLIKATWA------564 GELSU_84544 544 HPQASVYAVAERAADLIKACWA*------566 PHACH_37188 549 HPIAAIYILAERAADLIK------566 PHACH_135972 541 HPQAAVYILAERAADLVKQAWSH*------564 DICSQ_86071 548 HTMALVYIVAERAAELIKRTWD*------570 BJEAD_156054 542 HTTGPIYIIAERAADLVKQAWK*------564 BJEAD_171059 542 HPVAFIYVLAERAADLIKAAWK*------564 BJEAD_114954 536 HPMAFVYILAERASDLIKEAWSDYGDDY*------564 BJEAD_183896 542 HTVAFVYVLAERASDIIKEAWTHGGKST*------570 BJEAD_114902 541 HPVAFVYILAERAADLIKQTWNI*------564 BJEAD_52991 541 HPAAFLYIVAERAADLIKREWGLKL*------566 BJEAD_245297 543 HTVAPVYIIAERAAHLIKLSWAIQNT*------569 BJEAD_71431 540 HTVAPVYIVAERAADLIKAAWGL*------563 BJEAD_171002 544 HPMAAIYLLAERGSDLIKQKWKL*------567 BJEAD_245049 542 HTMAPIYLLAERGADLIKSAWGLS*------566 BJEAD_66377 555 HPQAVIYTLAERAADLVKEDYALESFGSSSEAQRVAHDEL*------595 PHACH_6199 556 HIQAVVYIIAERAADLVKAQYGLAQTPVGGGGHAEC*------592 PHLBR_131358 543 HIQAIVYTVAERAADLIMTAYGL*------566 PHLBR_164178 539 HPQALVYMVAERAADLIKDAYGLSS*------564 RHOPL_108489 571 HLQAPVYGLAEQAAELIRGTYVNADAANATSSASASSSAPSATTSASSRKSAATVLTPRVLPAALGAVAAAALAAIAL 648 PHACH_131961 594 HTQSIAYAIGEKAADILKAEIAAQA*------669 RHOPL_128830 566 HTQSTTYAIAEKAAEFIKHSK*------587