Polyclonal Antibody to CD349 / FZD9 / Frizzled-9 - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP31110PU-N Polyclonal Antibody to CD349 / FZD9 / Frizzled-9 - Aff - Purified

Alternate names: Fz-9, hFz9 Quantity: 0.1 mg Concentration: 0.5 mg/ml Background: Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The Frizzled 9 gene is located within the Williams syndrome common deletion region of chromosome 7. Frizzled 9 is critical for hippocampal development and is likely to be a contributing factor to the neurodevelopmental and behavioral phenotype of patients with Williams syndrome. Expressed predominantly in adult and fetal brain, testis, eye, skeletal muscle and kidney. Moderately expressed in pancreas, thyroid, adrenal cortex, small intestine and stomach. Detected in fetal liver and kidney. Uniprot ID: O00144 NCBI: NP_003499.1 GeneID: 8326 Host: Goat Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_003499.1. Genename: FZD9 AA Sequence: C-TKTDPSLENPTH

Format: State: Liquid purified Ig fraction Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide Buffer System: Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA Applications: Peptide ELISA: Detection Limit: 1/32000. Western Blot: Preliminary experiments in Kidney lysates from Human, Mouse and Rat gave no specific signal but low background (at antibody concentration up to 1 µg/ml). Immunohistochemistry on Paraffin Sections: 5-10 µg/ml. In paraffin embedded Human Testis shows acrosomal staining of spermatids. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: Recognizes CD349 / FZD9. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat. Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130809 1 / 2 AP31110PU-N: Polyclonal Antibody to CD349 / FZD9 / Frizzled-9 - Aff - Purified

General Readings: 1. Winn RA, Van Scoyk M, Hammond M, Rodriguez K, Crossno JT, Heasley LE, et al. Antitumorigenic effect of Wnt 7a and Fzd 9 in non-small cell lung cancer cells is mediated through ERK-5-dependent activation of peroxisome proliferator-activated receptor gamma. J Biol Chem. 2006 Sep 15;281(37):26943-50. Epub 2006 Jul 11. PubMed PMID: 16835228. Pictures: AP31110PU-N Frizzled-9 antibody staining of Formalin-Fixed, Paraffin Embedded Human Testis at 5 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130809 2 / 2