ZOOLOGIA 27 (5): 795–802, October, 2010 doi: 10.1590/S1984-46702010000500016 A new of the S. catharinae species group from Central Brazil (Amphibia: Anura: )

José P. Pombal Jr1; Ronald R. Carvalho Jr2; Marco Antônio S. Canelas3 & Rogério P. Bastos4

1 Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro. Quinta da Boa Vista, 20940-040 Rio de Janeiro, RJ, Brazil. E-mail: [email protected] 2 Táxon Meio Ambiente – Estudos e Projetos. Rua Marco Aurélio de Miranda 406/903, Buritis, 30575-210 Belo Horizonte, MG, Brazil. E-mail: [email protected] 3 Herpeto Consultoria Ambiental. Rua Caraça 539, Serra, 30220-260 Belo Horizonte, MG, Brazil. E-mail: [email protected] 4 Departamento de Ecologia, Instituto de Ciências Biológicas, Universidade Federal de Goiás. Caixa Postal 131, 74001-970 Goiânia, GO, Brazil. E-mail: [email protected]

ABSTRACT. A new species of Scinax Wagler, 1830 belonging to the S. catharinae species group is described from the state of Goiás, in the Cerrado Biome, Brazil. Scinax skaios sp. nov. is characterized by short snout-vent length (males ranging 23.2-29.6 mm; females 30.7-36.1 mm), snout subovoid in dorsal view and protruding in lateral view; no tubercle on canthus rostralis; an inverted triangular interorbital blotch; no externally expanded vocal sac; dorsal skin texture moderately rugose; absence of a thick externally well differentiated inguinal gland; hidden areas of the thigh with vermiculate color pattern. The descriptions of the vocalizations are provided. KEY WORDS. ; Cerrado; Dendropsophini; new species; Scinax skaios sp. nov.; vocalization.

Scinax Wagler, 1830 is the largest genus of Hylinae with kautskyi (Carvalho-e-Silva & Peixoto, 1991), S. littoralis (Pombal about 90 species occurring from eastern and southern Mexico & Gordo, 1991), S. longilineus (B. Lutz, 1968), S. luizotavioi to Argentina and Uruguay, Trinidad and Tobago, and St. Lucia (Caramaschi & Kisteumacher, 1989), S. machadoi (Bokermann Island (FROST 2010). New species are frequently described (e.g. & Sazima, 1973), S. obtriangulatus (B. Lutz, 1973), S. ranki PUGLIESE et al. 2004, 2009, LIMA et al. 2005, BRASILEIRO et al. 2007, (Andrade & Cardoso, 1987), S. rizibilis (Bokermann, 1964), S. DRUMMOND et al. 2007). FAIVOVICH et al. (2005) based on mo- strigilatus (Spix, 1824), S. trapicheiroi (B. Lutz, 1954), and S. lecular characters and some morphological features recognized tripui Lourenço, Nascimento & Pires, 2009. two clades: the S. catharinae clade and the S. ruber clade. Within During field work in central Brazil in 2005, we (RRC and the S. catharinae clade, they recognized the S. catharinae and MASC) collected specimens of the Scinax catharinae species the S. perpusillus species groups. As currently recognized, the group at Santa Rita do Novo Destino, state of Goiás, Central S. catharinae species group is the largest in the genus, com- Brazil. These are different from all other recognized taxa in the prising 28 species distributed in eastern and central Brazil, species group and are described herein. This new species and S. northeastern Argentina, southern Paraguay, and Uruguay centralis are the most inland taxa found in gallery forest from (FROST 2010). Most species are found in the Atlantic Rainforest Cerrado Domain, in Brazil (see POMBAL & BASTOS 1996). Domain (sensu AB’SÁBER 1977) and are typically forest dwell- ers. The species presently allocated to this species group are S. MATERIAL AND METHODS agilis (Cruz & Peixoto, 1983), S. albicans (Bokermann, 1967), S. angrensis (B. Lutz, 1973), S. argyreornatus (Miranda-Ribeiro, Specimens used in the description or examined for com- 1926), S. ariadne (Bokermann, 1967), S. aromothyella Faivovich, parisons are deposited in theAdolpho Lutz Collection (AL-MN, 2005, S. berthae (Barrio, 1962), S. brieni (De Witte, 1930), S. housed in Museu Nacional, Rio de Janeiro, Brazil), Museu de canastrensis (Cardoso & Haddad, 1982), S. carnevallii Ciências Naturais da PUC Minas (MCNAM, Belo Horizonte, (Caramaschi & Kisteumacher, 1989), S. catharinae (Boulenger, Minas Gerais, Brazil), and Museu Nacional (MNRJ, Rio de 1888), S. centralis Pombal & Bastos, 1996, S. flavoguttatus (A. Janeiro, Brazil). Lutz & B. Lutz, 1939), S. heyeri (Peixoto & Weygoldt in The following measurements were taken with calipers: Weygoldt, 1986), S. hiemalis (Haddad & Pombal, 1987), S. SVL (snout-vent length), HW (head width), HL (head length), humilis (B. Lutz, 1954), S. jureia (Pombal & Gordo, 1991), S. THL (thigh length), TBL (tibia length), and FL (foot length).

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Six others variables, ED (eye diameter), IOD (interorbital dis- Diagnosis. A small species (SVL of males 23.2-27.6 mm tance), IND (internarial distance), END (eye-nostril distance), and females 29.7-36.1 mm) belonging to the Scinax catharinae NSD (nostril-snout distance), and TD (tympanum diameter), species group, characterized by snout subovoid in dorsal view were measured using an ocular micrometer in a Zeiss stereomi- and protruding in lateral view; no tubercle on the canthus croscope. All measurements are in millimeters and followed rostralis; an inverted triangular interorbital blotch; no vocal DUELLMAN (2001) and CEI (1980). Webbing formula notation sac expanded externally; dorsal skin texture moderately rug- follows SAVAGE & HEYER (1967), as modified by MYERS & DUELLMAN ose; absence of a thick externally well differentiated inguinal (1982). The sex was determined by smaller size and forearm gland; hidden areas of thigh with vermiculate pattern. more robust in males and in females was made a small lateral Comparison with other species. Scinax skaios can be sepa- incision on abdomen to visualize the egg mass. rated from S. agilis, S. argyreornatus, S. berthae, S. centralis, and S. Vocalizations of two males were recorded with a Panasonic machadoi by the greater male SVL (male combined SVL 13.5- RQ-L309 portable tape recorder. The vocalizations were edited 22.2 mm in those species; BOKERMANN & SAZIMA 1973, CRUZ & at a sampling frequency of 22 kHz and 16 bits resolution with a PEIXOTO 1983, POMBAL & BASTOS 1996, FAIVOVICH 2005). By con- PC-Pentium computer and analyzed with Avisoft-Sonagraph trast, can be distinguished from S. ariadne and S. catharinae by Light and Cool Edit 96 software. The oscilogram and sonogram the smaller male SVL (male combined SVL 33.0-36.5; B. LUTZ was produced with 256 points, 50% overlap, and in flat top. 1973, where S. ariadne was called Hyla catharinae bocainensis). It can be separated from S. albicans, S. flavoguttatus, S. longilineus, and S. strigilatus by the smaller female (female combined SVL Scinax skaios sp. nov. 36.9-43.1; B. Lutz 1973, where S. albicans was called of Hyla catharinae opalina; ANDRADE & CARDOSO 1990, PIMENTA et al. 2007). Figs 1-6 Scinax skaios further differs from S. longilineus by the less rugose Holotype. MNRJ 54471, female, collected at Anglo Ameri- dorsal skin, (moderately rugose in S. skaios). The lack of thick, can do Brasil (15º05’17”S, 49º02’23”W), municipality of Santa externally well differentiated inguinal glands distinguishes S. Rita do Novo Destino, state of Goiás, Brazil, 28.VI.2005, Ronald skaios from S. centralis and S. hiemalis (POMBAL & BASTOS 1996, R. Carvalho Jr & Marco Antônio S. Canelas leg. Paratopotypes. HADDAD & POMBAL 1987). The slightly visible and inverted trian- MCNAM 6220-6221 (one female and one male), 22.VI.2005; gular interocular blotch differentiates the new species from S. MCNAM 6222-6223 (one male and one female), 28.VI.2005; angrensis, S. flavoguttatus, S. heyeri, S. humilis, S. kaustkyi, S. MCNAM 6225 (one female), 28.VI.2005; MCNAM 6227 (one littoralis, and S. tripui (in these species the interocular blotch is female), 28.VI.2005; MCNAM 6229-31 (three males), 28.VI.2005; W-shaped, sometimes interrupted; (B. LUTZ 1973, PEIXOTO & MCNAM 6233 (one female), 28.VI.2005; MNRJ 54472-74 (one WEYGODT 1987, CARAMASCHI & KISTEUMACHER 1989, CARVALHO E SILVA male and two females), 28.VI.2005. All collected at the type lo- & PEIXOTO 1991, POMBAL & GORDO 1991, LOURENÇO et al. 2009) and cality by the same collectors. from S. trapicheiroi, in which the interocular blotch is very large,

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Figures 1-2. Scinax skaios sp. nov., holotype, MNRJ 54471, female SVL 29.6 mm, in dorsal (1) and ventral (2) views.

ZOOLOGIA 27 (5): 795–802, October, 2010 A new Scinax of the S. catharinae species group from Central Brazil 797

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3

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Figures 3-6. Scinax skaios sp. nov., holotype (MNRJ 54471): dorsal (3) and lateral (4) views of head; ventral views of hand (5) and foot (6). Scale = 5 mm.

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extending well onto to the scapular region (B. LUTZ 1973). Fur- tubercles; outer metacarpal tubercle large and bifid; inner metac- ther, S. skaios differs from S. flavoguttaus and S. heyeri by lacking arpal tubercle large, nearly ovoid; finger discs medium-sized, vivid orange color on the hidden surfaces of the thigh (B. LUTZ nearly round; disc of finger I smaller than others; webbing be- 1973, PEIXOTO & WEYGOLDT 1987). The lack of an externally dis- tween fingers absent. Legs slender, tibia longer than thigh; sum tended vocal sac distinguishes S. aromothyella from the new spe- of thigh and tibia lengths greater than SVL; toes slender; toe cies (FAIVOVICH 2005). The absence of an enlarged nuptial pad length I

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Table I. Measurements (mm) of holotype (MNRJ 54474) and paratypes of S. skaios sp. nov. (M) male and (F) female. Individual Sex SVL HL HW ED TD IOD IND END NSD THL TBL FL MCNAM 6221 M 27.0 10.6 9.5 3.3 1.2 2.9 2.0 2.6 1.0 14.5 16.1 13.0 MCNAM 6222 M 26.0 10.2 9.2 3.5 1.2 3.0 2.2 2.3 1.1 12.5 14.1 11.6 MCNAM 6227 M 23.2 9.4 8.0 2.9 1.5 2.5 1.9 2.0 1.5 12.4 14.0 10.7 MCNAM 6229 M 25.2 9.6 9.0 3.2 1.3 3.0 2.0 2.3 1.1 12.4 15.7 11.7 MCNAM 6230 M 25.9 9.9 8.8 3.2 1.5 3.0 2.0 2.3 1.1 14.5 15.3 12.1 MCNAM 6231 M 26.7 10.1 9.1 3.2 1.3 2.8 2.3 2.4 1.0 13.3 15.7 12.6 MNRJ 54473 M 25.1 10.0 8.5 3.1 1.4 2.7 2.2 2.1 1.1 12.7 14.6 11.5 MNRJ 54474 M 25.1 9.7 8.8 3.2 1.1 3.1 2.2 2.1 1.1 13.0 15.0 11.4 MNRJ 54471 F 29.7 11.4 11.0 3.2 1.6 2.8 2.0 2.3 0.5 16.0 18.1 13.7 MCNAM 6220 F 33.9 12.2 10.5 3.2 1.6 3.1 2.5 3.0 1.2 16.3 19.1 14.7 MCNAM 6223 F 36.1 13.6 12.9 4.0 2.0 3.7 3.0 3.0 1.9 18.3 20.3 15.6 MCNAM 6225 F 31.5 11.8 10.7 3.5 1.6 3.2 2.4 3.2 1.2 16.2 18.4 13.7 MCNAM 6233 F 30.7 11.2 10.2 3.6 1.1 3.2 2.8 2.9 1.3 16.9 18.3 12.5 MNRJ 54472 F 31.2 11.8 10.9 3.4 1.6 3.4 2.6 2.9 1.2 15.9 18.4 14.2

sum varied from smooth to tuberculate. About 75% of speci- vocalizations. With one exception (see below), the advertisement mens (males and females) have a clear line between eyes. Su- call of Scinax skaios differs from all calls described so far by hav- pernumerary tubercles vary in number. ing more notes per call. The advertisement call of S. argyreornatus Vocalizations. Two types of vocalizations (Figs 8-11) were shares this feature, but it can be distinguished by its lower domi- recorded: a long call (here considered the advertisement call nant frequency (POMBAL et al. 1995). because it was more frequent) and a short call (named isolated and natural history. The Cerrado Biome covers call). Two advertisement calls were recorded from two males. about 2 million km2, representing 22% of Brazil, plus small ar- The duration of the two advertisement calls was 4.42 s and 7.9 eas in eastern Bolivia and northwestern Paraguay. It extends s, which presented 42 and 73 notes. Average note duration was from the southern borders of the Amazonian forest to areas in 0.27± 0.012 s (0.013-0.056, N = 10 notes), emitted on an inter- the southern states of São Paulo and Paraná. The distribution val of 0.087 ± 0.053 s (0.036-0.168 s, N = 10 intervals). The of the Cerrado is coincident with the plateau of central Brazil. calls presented 312 or 612 pulses and notes had 9.2 ± 3.33 pulses Narrow forest strips are called “gallery forest” because the (5-16, N = 10 notes). The dominant frequencies were 2.205 crowns of the trees form a gallery over the water of small rivers and 2.243 Hz. or rivulets. The central Brazilian riverine forests represent flo- Three isolated calls were recorded from two males and ristic intrusions of the Amazonian and/or Atlantic forest into showed pulsed structure with duration of 0.036 ± 0.017s (0.017- the Cerrado Domain (OLIVEIRA-FILHO & RATTER 2002). Scinax skaios 0.049s, N = 3 calls) and 14.43 ± 5,69 pulses (8-19 pulses, n = 3 is the second species of the S. catharinae species group recorded calls). The dominant frequency varied from 2.310 to 2.348 Hz only in the Cerrado biome (POMBAL & BASTOS 1996). Like S. cen- (X = 2,323 ± 52 Hz). Vocalizations of the following species of the tralis, S. skaios is encountered in gallery forests. Scinax catharinae group are described: S. albicans (HEYER 1980), S. Males vocalized close to the rivulets in the gallery forests argyreornatus (BOKERMANN 1966, POMBAL et al. 1995), S. canastrensis or in the cerrado near gallery forests. As vocalization site, the (CARDOSO & HADDAD 1982), S. catharinae (HEYER 1980), S. centralis males utilized branches of marginal vegetation, rocks or ground (POMBAL & BASTOS 1996), S. hiemalis (HADDAD & POMBAL 1987), S. (0-1.0 m above the water). The vocalizations could be heard machadoi (BOKERMANN & SAZIMA 1973), S. ranki (ANDRADE & CARDOSO staring around 21:00 h. The amplexus is axilar. 1987), S. rizibilis (BOKERMANN 1964, POMBAL et al. 1995, BASTOS & Distribution. The new species is known only from the HADDAD 2002). Vocalizations of species this group are complex type locality in the municipality of Santa Rita do Novo Destino, (POMBAL et al. 1995, POMBAL & BASTOS 1996). Given that the avail- state of Goiás, Brazil. able descriptions are based on different equipments and the Etymology. Skaios is a Greek word meaning western. Most acoustic parameters differ between studies (see POMBAL et al. 1995 of the species of the Scinax catharinae species group occur in for revision) we have avoided a detailed comparison among these the Atlantic Forest in eastern Brazil (POMBAL & BASTOS 1996).

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9 Figures 8-9. Spectrogram of the advertisement call and isolated call of S. skaios sp. nov. (MCNAM 6221) from type locality at municipal- ity of Santa Rita do Novo Destino, state of Goiás, Brazil, recorded on 22 June 2005. Air temperature 23.5° C.

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Figures 10-11. Waveform of the advertisement call and isolated call of S. skaios sp. nov. (MCNAM 6221) from type locality at municipal- ity of Santa Rita do Novo Destino, state of Goiás, Brazil, recorded on 22 June 2005. Air temperature 23.5° C. The time is 4.5s.

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ACKNOWLEDGEMENTS CEI, J.M. 1980. Amphibians of Argentina. Monitore Zoologico Italiano (N.S.), Monografia, 2: 1-609. We thank D.S. Fernandes, A.C.C. Lourenço, and M.W. CRUZ, C.A.G. & O.L. PEIXOTO. 1983 Uma nova espécie de Hyla Cardoso for helpful suggestions on the manuscript; P.R. do Estado do Espírito Santo, Brasil (Amphibia, Anura, Nascimento for the line drawings; D. Baêta and A.C.C. Lourenço Hylidae). Revista Brasileira de Biologia 42 (4): 721-724. for the pictures 1 and 2. We thank Anglo American do Brasil DRUMMOND, L.O.; D. BAÊTA & M.R.S. PIRES. 2007. A new species of for the license to access the study area and for financial sup- Scinax (Anura, Hylidae) of the S. ruber clade from Minas port. The Instituto Brasileiro do Meio Ambiente e dos Recursos Gerais, Brazil. Zootaxa 1612: 45-53. Naturais Renováveis/Centro de Conservação e Manejo de DUELLMAN, W.E. 2001. Hylid of Middle America. Répteis e Anfíbios (Ibama/RAN) provided capture/transporta- Lawrence, The Society for the Study of Amphibians and tion license 079/2005-CGFAU/LIC. The Conselho Nacional de Reptiles, 1158p. Desenvolvimento Científico e Tecnológico (CNPq) provided FAIVOVICH, J. 2005. A new species of Scinax (Anura: Hylidae) from fellowships to JPP and RPB. Financial supports were provided Misiones, Argentina. Herpetologica 61 (1): 69-77. by ANPCy PICT 2007/2202, CNPq, Funape/UFG, Fundação FAIVOVICH, J.; C.F.B. HADDAD; P.C.A. GARCIA; D.R. FROST; J.A. Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de CAMPBELL & W.C. WHEELER. 2005. Systematic review of the Janeiro (FAPERJ), and CNPq. family Hylidae, with special reference to Hylinae: phylogenetic analysis and taxonomic revision. Bulletin of LITERATURE CITED the American Museum of Natural History 294: 1-240. AB’SABER, A.N. 1977.Os domínios morfoclimáticos na América FROST, D. R. 2010. Species of the World: an Online do Sul: primeira aproximação. 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POMBAL JR, J.P. & R.P.BASTOS. 1996. Nova espécie de Scinax Wagler, and neotype designation for a “ghost” taxon. Zootaxa 1441: 1830 do Brasil Central (Amphibia, Anura, Hylidae). Bole- 35-49. tim do Museu Nacional (N.S.) Zoologia 371: 1-11. PUGLIESE, A.; D. BAÊTA, & J.P. POMBAL JR. 2009. A new species of POMBAL JR, J.P.; R.P.BASTOS & C.F.B. HADDAD. 1995. Vocalizações Scinax (Anura: Hylidae) from rocky montane fields in de algumas espécies do gênero Scinax (Anura, Hylidae) do Southeastern and Central Brazil. Zootaxa 2269: 53-64. sudeste do Brasil e comentários taxonômicos. Naturalia 20: PUGLIESE, A.; J.P. POMBAL JR & I. SAZIMA, I. 2004. A new species of 213-225. Scinax (Anura: Hylidae) from rocky montane fields of Serra POMBAL JR, J.P & M. GORDO. 1991. Duas novas espécies de Hyla do Cipó, Southeastern Brazil. Zootaxa 688: 1-15. da Floresta Atlântica no Estado de São Paulo (Amphibia, SAVAGE, J.M. & W.R. HEYER. 1967. Variation and distribution Anura). Memórias do Instituto Butantan 53(1): 135-144. in the tree-frog genus Phyllomedusa in Costa Rica, Cen- PIMENTA, B.V.S.; J. FAIVOVICH & J.P. POMBAL JR. 2007. On the identity tral America. Beitraege zur Neotropischen Fauna 5: 11- of Hyla strigilata Spix, 1824 (Anura: Hylidae): redescription 131.

Appendix 1. Addional examined specimens.

Scinax agilis: BRAZIL, Bahia: Belmonte MNRJ 46853-54; Espírito Santo: Setiba MNRJ 43094-95. Scinax albicans: BRAZIL, Rio de Janeiro: Duque de Caxias MNRJ 53670-87; Teresópolis MNRJ 53940-47. Scinax argyreornatus: BRAZIL, Bahia: Canavieiras MNRJ 40303- 08; Espírito Santo: Santa Teresa MNRJ 28360-72; Sooretama MNRJ 42910-17; Rio de Janeiro: Angra dos Reis MNRJ 49656; Itaguaí MNRJ 32036-42. Scinax ariadne: São Paulo: São José do Barreiro, Serra da Bocaina MNRJ 4051 (paratype). Scinax aromothyella: ARGENTINA, Misiones, Departamento de Guarany, San Vicente MNRJ 56445 (paratype). Scinax berthae: ARGENTINA: Buenos Aires MNRJ 3590 (paratype); BRAZIL, Rio Grande do Sul: Santa Maria MNRJ 39897; São Paulo, Botucatu MNRJ 40581. Scinax canastrensis: BRAZIL, Minas Gerais: São Roque de Minas MNRJ 4147 (holotype), MNRJ 4148 (paratype). Scinax carnevallii: BRAZIL, Minas Gerais: Caratinga MNRJ 4201-09 (paratypes); Marliéria MNRJ 4182 (holotype), MNRJ 4183-85, 4187-200 (paratypes). Scinax brieni: BRAZIL, São Paulo: Paranapiacaba AL-MN 2592-94; MNRJ 54428-29. Scinax carnevallii: BRAZIL, Minas Gerais: Caratinga MNRJ 4201-09 (paratypes); Marliéria MNRJ 4182 (holotype), MNRJ 4183-84 (paratypes), MNRJ 4185 (paratype). Scinax centralis: BRAZIL, Goiás: Silvânia MNRJ 17465 (holotype) 17466-75 (paratypes). Scinax flavoguttatus: BRASIL: Rio de Janeiro: Duque de Caxias MNRJ 53688-03; Teresópolis MNRJ 53950-58; São Paulo, Bananal, Serra da Bocaina MNRJ 23404-07. Scinax jureia: BRAZIL, São Paulo: Iguape MNRJ 14202-03 (paratypes). Scinax kautskyi: BRAZIL, Espírito Santo: Aracruz MNRJ 39785-98. Scinax hiemalis: BRAZIL, São Paulo: Botucatu MNRJ 23447-52, MNRJ 40008-09. Scinax humilis: BRAZIL, Rio de Janeiro: Cachoeirra de Macacu MNRJ 40082-95; Duque de Caxias MNRJ 2248 (lectoptype), MNRJ 1478 (paralectotype), 53715-17; Rio de Janeiro MNRJ 40178-81. Scinax littoralis: BRAZIL, São Paulo: Iguape MNRJ 46993. Scinax longilineus: BRAZIL, Minas Gerais: Belo Horizonte MNRJ 16003-07, 30966-69; Poços Caldas MNRJ 4060 (holotype), MNRJ 44618. Scinax luizotavioi: BRAZIL, Minas Gerais: Lima Duarte 36552-57; São Gonçalo do Rio Abaixo MNRJ 4473-4508 (paratypes); Santa Bárbara, Caraça MNRJ 4210 (holotype), 4211-15 (paratypes), 49684-88. Scinax machadoi: BRAZIL, Minas Gerais: Jaboticatubas MNRJ 17476-77 (paratypes), MNRJ 39696. Scinax obriangulatus: BRAZIL, Minas Gerais: Alto Itatiaia MNRJ 3707, 14675-81, 54404-07, 54408, 54409-12, 54413, 54414-16. Scinax trapicheiroi: BRAZIL, Rio de Janeiro: Rio de Janeiro MNRJ 3623-25 (paralectotypes), MNRJ 27356-58, MNRJ 34883-84, MNRJ 47172-73. Scinax strigilatus: BRAZIL, Bahia: Belmonte MNRJ 38099; Ibirapiranga MNRJ 38098 (neotype); Jussari MNRJ 44946, 44948-49. Scinax ranki: BRAZIL, Minas Gerais: Poços de Caldas MNRJ 49657. Scinax rizibilis: BRAZIL, São Paulo: Paranapiacaba MNRJ 4046, 14216, MNRJ40560-66; Ribeirão Branco MNRJ 18224-25; Ribeirão Grande MNRJ 28131-52; Santa Catarina: São Bento do Sul MNRJ 3629-30, 36349 (syntypes of Hyla mirim). Scinax tripui: BRAZIL, Minas Gerais: Ouro Preto MNRJ 42890 (holotype), 48762-67 (paratypes), 48743-45 (paratypes).

Submitted: 05.X.2009; Accepted: 19.IX.2010. Editorial responsibility: Ana Lúcia da C. Prudente

ZOOLOGIA 27 (5): 795–802, October, 2010