Efficient Professional and Antibody Platforms Karyopherin alpha-6 Antibody Basic information: Catalog No.: UPA61311 Source: Rabbit Size: 50ul/100ul Clonality: polyclonal Concentration: 1mg/ml Isotype: Rabbit IgG Purification: affinity purified by Protein A Useful Information: Applications: WB:1:500-2000 Reactivity: Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep Specificity: This antibody recognizes Karyopherin alpha-6 protein. Immunogen: KLH conjugated synthetic peptide derived from human KPNA6 101-200/536 Functions in nuclear protein import as an adapter protein for nuclear re- ceptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the /substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the Description: nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from im- portin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Uniprot: O60684 Human BiowMW: 60 KDa Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-KPNA6 at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Sample: DU145 Cell (Human) Lysate at 40 ug Primary: Anti-KPNA6 at 1/300 dilution Second- ary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Ob- served band size: 63 kD

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]