UBE2L3 Antibody Cat. No.: 29-823

UBE2L3 Antibody

Antibody used in WB on Human Jurkat 1.25 ug/ml.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human UBE2L3.

TESTED APPLICATIONS: ELISA, IHC, WB

UBE2L3 antibody can be used for detection of UBE2L3 by ELISA at 1:312500. UBE2L3 APPLICATIONS: antibody can be used for detection of UBE2L3 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate

PREDICTED MOLECULAR 75 kDa, 75 kDa WEIGHT:

September 29, 2021 1 https://www.prosci-inc.com/ube2l3-antibody-29-823.html Properties

PURIFICATION: Antibody is purified by A chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: UBE2L3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: UBE2L3

ALTERNATE NAMES: UBE2L3, E2-F1, L-UBC, UBCH7, UbcM4

ACCESSION NO.: NP_003338

PROTEIN GI NO.: 4507789

GENE ID: 7332

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

The modification of with is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin- conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of , c-Fos, and the NF-kB precursor p105 in vitro.This encodes a protein that has been identified as a component of NuRD, a nucleosome remodeling deacetylase complex identified in the nucleus of human cells. It shows a very broad expression pattern and is strongly expressed in many tissues. It may represent one BACKGROUND: member of a small gene family that encode different but related proteins involved either directly or indirectly in transcriptional regulation. Their indirect effects on transcriptional regulation may include chromatin remodeling. It is closely related to another member of this family, a protein that has been correlated with the metastatic potential of certain carcinomas. These two proteins are so closely related that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. One of the proteins known to be a target protein for this gene product is p53. Deacteylation of p53 is correlated with a loss of growth inhibition in transformed cells supporting a connection between these gene family members and metastasis.

September 29, 2021 2 https://www.prosci-inc.com/ube2l3-antibody-29-823.html REFERENCES: 1) Yao, Y.L. (2003) J. Biol. Chem. 278 (43), 42560-42568.

ANTIBODIES FOR RESEARCH USE ONLY.

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