Polyclonal Antibody to APAF1 (N-Term) - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP23392PU-N Polyclonal Antibody to APAF1 (N-term) - Aff - Purified

Alternate names: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413 Quantity: 0.1 mg Background: Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23.1 It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis.2 This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9.3 Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death.4 Additionally, APAF1 has been shown to interact with NLRP1,5 Caspase-9,5, 6, 7, 8, 9, 10 APIP,6 BCL2-like 18, 9 and HSPA4.10 Uniprot ID: O14727 NCBI: NP_001151 GeneID: 317 Host / Isotype: Rabbit / IgG Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human APAF1 Remarks: Sequence is identical to the related rat and mouse sequence. Format: State: Lyophilized Ig fraction Purification: Immunogen affinity purified Buffer System: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3 Reconstitution: 0.2ml of distilled water will yield a concentration of 500μg/ml. Applications: Western blot: 1 μg/ml. Immunohistochemistry on paraffin sections 2 μg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody detects APAF1 (N-term). No cross reactivity with other proteins. Species Reactivity: Tested: Human, rat, mouse. Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20130208 1 / 2 AP23392PU-N: Polyclonal Antibody to APAF1 (N-term) - Aff - Purified

Pictures: Immunohistochemistry with APAF1 Polyclonal Antibody

Western blot with APAF1 Polyclonal Antibody

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20130208 2 / 2