For Research Use Only

Transportin-1 Polyclonal antibody www.ptglab.com Catalog Number:20679-1-AP 1 Publications

Catalog Number: GenBank Accession Number: Purification Method: Basic Information 20679-1-AP NM_002270 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 300 μg/ml by 3842 WB 1:200-1:1000 Nanodrop and 160 μg/ml by Bradford Full Name: method using BSA as the standard; transportin 1 Source: Calculated MW: Rabbit 102 kDa Isotype: Observed MW: IgG 102 kDa

Applications Tested Applications: Positive Controls: WB, ELISA WB : HeLa cells, HEK-293 cells, L02 cells, mouse liver Cited Applications: tissue IF Species Specificity: human, mouse Cited Species: human

TNPO1, also named as KPNB2, MIP1, TRN and MIP, belongs to the importin beta family. TNPO1 functions in nuclear Background Information protein import as nuclear transport receptor. It serves as receptor for nuclear localization signals (NLS) in cargo substrates. TNPO1 is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. TNPO1 is involved in nuclear import of M9-containing proteins. In vitro, it binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. It is involved in hnRNP A1/A2 nuclear export. TNPO1 mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. It binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, it mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. The antibody is specific to TNPO1.

Notable Publications Author Pubmed ID Journal Application Tatyana A Shelkovnikova 31875556 Cell Rep IF

Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data

HeLa cells were subjected to SDS PAGE followed by western blot with 20679-1-AP (Transportin-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.