DOCSLIB.ORG

Preserving Amniotic Fluid Stem Cells

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Preserving Amniotic Fluid Stem Cells “I wanted as many options as possible for my Prices and child’s future well-being .When I spoke with my Preserving Amniotic Payment Options doctor, he said that I could preserve amniotic fluid stem cells during my scheduled amniocentesis ”. Fluid Stem Cells First Year Fee* – Total $1,650 —Biocell Center customer Initial Payment . $399 and mother of two Biological Insurance Remainder Due Upon “I see this service as very valuable for moms for the Future Successful Sample Processing . $1,251. and their babies to preserve cells that are only available during pregnancy . And as a doctor, I Annual Fees appreciate the ability to give this extra service and Storage Fee (after 1st Year) . $120 option to my patients without any changes to the standard 2nd trimester amniocentesis ”. ** Optional Prepayment Plan —Chief of Maternal Fetal Medicine, Prepaid 19 year contract . $2,800. largest community-based hospital network in New England *First Year Fee covers the cost of the collection kit, all shipment costs, sample processing and preparation for “Amniotic fluid is one of the best sources of stem cryopreservation, and the first year of storage cells that may be the next frontier in stem cell **Prepayment covers the cost of the collection kit, all shipment costs, sample processing and preparation for cryopreservation, therapy . There are dozens of on-going studies in and 19 years of storage at a total savings of over $1,000! cellular therapies and tissue regeneration using Other payment plans are available . this valuable biological material ”. —Chief Medical Officer, Genetic Testing Laboratory Call Today! Consider the unique opportunity to preserve amniotic fluid stem cells during your AMNIOCENTESIS Stem cells are currently used for a variety of medical conditions and new applications [email protected] are being discovered rapidly. Although there is no guarantee that the FDA will approve any potential future medical applications and banking of amniotic fluid does not Fax: 1.781.395.0602 guarantee that these cells will be applicable to a particular medical condition, many clinical trials are on-going worldwide that may result in human therapies. Preserving www.biocellcenter.com amniotic fluid stem cells offers great hope for the future. Only your healthcare provider can suggest whether you should perform the 200 Boston Ave. Medford, MA 02155 amniocentesis procedure for prenatal genetic diagnosis during your pregnancy. Amniotic fluid stem cell Why Preserve Biological insurance preservation is a private Amniotic Fluid? for the Future tissue banking service offered If you are scheduled for an amniocentesis, you Your amniotic fluid sample can be stored for by Biocell Center Corporation have a unique opportunity to preserve amniotic decades and retrieved at any time. It is easily and is available to women fluid stem cells for your child and your family. accessible to your child from birth to well into adulthood. scheduled for a 2nd trimester Amniotic fluid contains stem cells that can genetic amniocentesis. 1 grow into many different tissues such as bone, muscle, cartilage, nerves, kidney, liver and skin . How Our Process Works: Amniotic stem cells may have numerous 2 future medical applications, including in Call Biocell Center for your tissue regeneration, organ transplantation, sample collection kit and and potentially in neurodegenerative complete instructions diseases and diabetes . Bring the kit with you Amniotic fluid stem cells are a perfect 3 to your amniocentesis match to your child and may also be appointment compatible with other family members . Mail your sample to Biocell Center Amniotic fluid stem cells can only be 4 obtained and saved NOW, during your Receive your sample scheduled amniocentesis! acceptance certificate What is Amniocentesis? Amniocentesis is a diagnostic procedure that is performed during pregnancy based on the recommendation of your doctor, and is primarily used for prenatal diagnosis of genetic abnormalities. Amniocentesis consists of the extraction of amniotic fluid by a thin needle inserted Biocell Center is the #1 amniotic fluid stem cell bank in the world. into the abdomen. It is typically performed between the 15th and 20th week of With global operations and experience, you can trust Biocell Center pregnancy. with your precious amniotic fluid stem cells. Amniotic fluid needed for stem cell preservation is collected during amniocentesis Call Biocell Center or talk to your doctor today with no change to the standard procedure. 1.866.BIO.2720 (1.866.246.2720).
Load more

Recommended publications
  • BMP-Treated Human Embryonic Stem Cells Transcriptionally Resemble Amnion Cells in the Monkey Embryo
    bioRxiv preprint doi: https://doi.org/10.1101/2021.01.21.427650; this version posted January 22, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo Sapna Chhabra1,2,3, Aryeh Warmflash2,4* 1Systems Synthetic and Physical Biology graduate program, 2Department of Biosciences, 4Department of Bioengineering, Rice University, Houston, TX 77005 3Present address: Developmental Biology Unit, EMBL Heidelberg. *Correspondence to AW: [email protected] Abstract Human embryonic stem cells (hESCs) possess an immense potential to generate clinically relevant cell types and unveil mechanisms underlying early human development. However, using hESCs for discovery or translation requires accurately identifying differentiated cell types through comparison with their in vivo counterparts. Here, we set out to determine the identity of much debated BMP-treated hESCs by comparing their transcriptome to the recently published single cell transcriptomes of early human embryos in the study Xiang et al 2019. Our analyses reveal several discrepancies in the published human embryo dataset, including misclassification of putative amnion, intermediate and inner cell mass cells. These misclassifications primarily resulted from similarities in pseudogene expression, highlighting the need to carefully consider gene lists when making comparisons between cell types. In the absence of a relevant human dataset, we utilized the recently published single cell transcriptome of the early post implantation monkey embryo to discern the identity of BMP-treated hESCs.
    [Show full text]
  • Amnion-Derived Cells As a Reliable Resource for Next-Generation Regenerative Medicine T
    Placenta 84 (2019) 50–56 Contents lists available at ScienceDirect Placenta journal homepage: www.elsevier.com/locate/placenta Amnion-derived cells as a reliable resource for next-generation regenerative medicine T Akihiro Umezawaa,*, Akihiro Hasegawaa,b, Momoko Inouea,b, Akiko Tanuma-Takahashia,b, Kazuhiro Kajiwaraa,b, Hatsune Makinoa, Emi Chikazawaa, Aikou Okamotob a Department of Reproductive Biology, National Center for Child Health and Development, Tokyo, 157-8535, Japan b Department of Obstetrics and Gynecology, The Jikei University School of Medicine, Tokyo, 105-8471, Japan ARTICLE INFO ABSTRACT Keywords: The placenta is composed of the amnion, chorionic plate, villous and smooth chorion, decidua basalis, and HLA umbilical cord. The amnion is a readily obtainable source of a large number of cells and cell types, including Mesenchymal stem cell epithelium, mesenchyme, and endothelium, and is thus an allogeneic resource for regenerative medicine. Cell-based therapy Endothelial cells are obtained from large arteries and veins in the amniotic membrane as well as the umbilical Stromal cell cord. The amnion-derived cells exhibit transdifferentiation capabilities, including chondrogenesis and cardio- Epithelial cell myogenesis, by introduction of transcription factors, in addition to their original and potential phenotypes. The Direct reprogramming amnion is also a source for production of induced pluripotent stem cells (AM-iPSCs). The AM-iPSCs exhibit stable phenotypes, such as multipotency and immortality, and a unique gene expression pattern. Through the use of amnion-derived cells, as well as other placenta-derived cells, preclinical proof of concept has been achieved in a mouse model of muscular dystrophy. 1. Text manually separated (Fig. 1A–G).
    [Show full text]
  • Prenatal Transplantation of Human Amniotic Fluid Stem Cell Could
    www.nature.com/scientificreports OPEN Prenatal transplantation of human amniotic fuid stem cell could improve clinical outcome of type III spinal muscular atrophy in mice Steven W. Shaw1,2,3,10*, Shao‑Yu Peng4,10, Ching‑Chung Liang5, Tzu‑Yi Lin1, Po‑Jen Cheng1,5, T’sang‑T’ang Hsieh1,2, Hao‑Yu Chuang6,7, Paolo De Coppi8,9 & Anna L. David3 Spinal muscular atrophy (SMA) is a single gene disorder afecting motor function in uterus. Amniotic fuid is an alternative source of stem cell to ameliorate SMA. Therefore, this study aims to examine the therapeutic potential of Human amniotic fuid stem cell (hAFSC) for SMA. Our SMA model mice were generated by deletion of exon 7 of Smn gene and knock‑in of human SMN2. A total of 16 SMA model mice were injected with 1 × ­105 hAFSC in uterus, and the other 16 mice served as the negative control. Motor function was analyzed by three behavioral tests. Engraftment of hAFSC in organs were assessed by fow cytometry and RNA scope. Frequency of myocytes, neurons and innervated receptors were estimated by staining. With hAFSC transplantation, 15 fetuses survived (93.75% survival) and showed better performance in all motor function tests. Higher engraftment frequency were observed in muscle and liver. Besides, the muscle with hAFSC transplantation expressed much laminin α and PAX‑7. Signifcantly higher frequency of myocytes, neurons and innervated receptors were observed. In our study, hAFSC engrafted on neuromuscular organs and improved cellular and behavioral outcomes of SMA model mice. This fetal therapy could preserve the time window and treat in the uterus.
    [Show full text]
  • Amniotic Fluid Naturally Contains the Necessary “Ingredients” for Developing an Extracellular Matrix That Can Repair Damaged Tissue
    PalinGenAmniotic SportFlow Fluid - Supporting Scientific Rationale Russell Health, Inc. Mechanism of Action for AmnioticPalinGen FluidSportFlow Amniotic fluid naturally contains the necessary “ingredients” for developing an extracellular matrix that can repair damaged tissue. Amniotic fluid contains a number of components that are imperative in the development of this foundational extracellular matrix, such as collagen, which forms fibrils that provide structure for tissues like ligaments, tendons, and skin. In addition to collagen, cytokines, chemokines, and hyaluronan in amniotic fluid work together within the matrix to regulate inflammation, maximize communication, and initiate cell regrowth within the tissue. An Amniotic Fluid Injection is an injectable scaffold that utilizes a naturally formed mixture of bioactive molecules and solidifiable precursors found in pure amniotic fluid. By injecting Amniotic Fluid into defected joints or soft tissues, a newAmniotic 3D structure of regenerated healthy tissue is created. This entire process generally takes 3-6 weeks. References 1.Technology-Insight-Adult-Mesenchymal-Stem-Cells-for-Osteoarthritis-Therapy-Noth ​ 2. Potential use of the human amniotic membrane as a scaffold in human articular cartilage repair ​ 3. Amniotic Fluid: Not Just Fetal Urine Anymore. Mark A Underwood MD1, William M Gilbert MD2 and ​ Michael P Sherman MD1 4. Amniotic Fluid Cell Therapy to Relieve Disc-Related Low Back Pain and Its Efficacy Comparison with ​ Long-Acting Steroid Injection The following pages contain the previously listed references and additional supporting studies. ​ REVIEW www.nature.com/clinicalpractice/rheum Technology Insight: adult mesenchymal stem cells for osteoarthritis therapy Ulrich Nöth, Andre F Steinert and Rocky S Tuan* SUMMARY INTRODUCTION Osteoarthritis (OA), the most common form Despite the high prevalence and morbidity of osteoarthritis (OA), an of joint disease, is characterized by degenera- effective treatment for this disease is currently lacking.
    [Show full text]
  • Mechanisms of Human Embryo Development: from Cell Fate to Tissue Shape and Back Marta N
    © 2020. Published by The Company of Biologists Ltd | Development (2020) 147, dev190629. doi:10.1242/dev.190629 REVIEW Mechanisms of human embryo development: from cell fate to tissue shape and back Marta N. Shahbazi* ABSTRACT activated ion channels (Coste et al., 2010), mechanosensitive Gene regulatory networks and tissue morphogenetic events drive the transcription factors (Dupont et al., 2011) or directly by the nucleus emergence of shape and function: the pillars of embryo development. (Kirby and Lammerding, 2018). Once sensed, mechanical cues – Although model systems offer a window into the molecular biology of are transduced into biochemical signals a process known as cell fate and tissue shape, mechanistic studies of our own mechanotransduction (Chan et al., 2017). The conversion of development have so far been technically and ethically challenging. mechanical cues into biochemical signals leads to changes in However, recent technical developments provide the tools to gene expression and protein activity that control cell behaviour, cell describe, manipulate and mimic human embryos in a dish, thus fate specification and tissue patterning. opening a new avenue to exploring human development. Here, I Current consensus focuses on two main ideas to explain the discuss the evidence that supports a role for the crosstalk between emergence of tissue patterns in response to morphogen (see Glossary, ‘ ’ cell fate and tissue shape during early human embryogenesis. This is Box1)signals.Inthe positional information model (Wolpert, a critical developmental period, when the body plan is laid out and 1969), the concentration of a morphogen serves as a coordinate of the many pregnancies fail. Dissecting the basic mechanisms that position of a cell within a tissue.
    [Show full text]
  • Stem/Progenitor Cells in Fetuses and Newborns: Overview of Immunohistochemical Markers D
    Fanni et al. Cell Regeneration (2021) 10:22 https://doi.org/10.1186/s13619-021-00084-6 REVIEW Open Access Stem/progenitor cells in fetuses and newborns: overview of immunohistochemical markers D. Fanni1,2, C. Gerosa1,2, C. Loddo3, M. Castagnola4, V. Fanos3, M. Zaffanello5* and G. Faa1,2 Abstract Microanatomy of the vast majority of human organs at birth is characterized by marked differences as compared to adult organs, regarding their architecture and the cell types detectable at histology. In preterm neonates, these differences are even more evident, due to the lower level of organ maturation and to ongoing cell differentiation. One of the most remarkable finding in preterm tissues is the presence of huge amounts of stem/progenitor cells in multiple organs, including kidney, brain, heart, adrenals, and lungs. In other organs, such as liver, the completely different burden of cell types in preterm infants is mainly related to the different function of the liver during gestation, mainly focused on hematopoiesis, a function that is taken by bone marrow after birth. Our preliminary studies showed that the antigens expressed by stem/progenitors differ significantly from one organ to the next. Moreover, within each developing human tissue, reactivity for different stem cell markers also changes during gestation, according with the multiple differentiation steps encountered by each progenitor during development. A better knowledge of stem/progenitor cells of preterms will allow neonatologists to boost preterm organ maturation, favoring the differentiation of the multiple cells types that characterize each organ in at term neonates. Keywords: Fetus, Immunohistochemical analysis, Newborn, Progenitor cells, Stem cells Background markers have been proposed during the years.
    [Show full text]
  • BMP) Journal Homepage
    DOI 10.7603/s40855-015-0002-1 PROGRESS IN STEM CELL 2015, 2(1):58-64 Available online at http://www.springer.com/globalsciencejournals BioMedPress (BMP) Journal homepage: http://www.cellstemcell.org http://www.biomedpress.org/psc Research article Demons resurrected from Angels! What are or are not the molecular clues of the rising of the teratoma or teratocarcinoma from human amniotic stem cell? Md. Shaifur Rahman1*, Madhuri Haque2, S. M. Asaduzzaman1 1Tissue Banking and Biomaterial Research Unit (TBBRU), Atomic Energy Research Establishment (AERE), 1349 Dhaka, Bangladesh 2Department of Biotechnology and Genetic Engineering, Jahangirnagar University, 1342 Dhaka, Bangladesh A R T I C L E I N F O Article history: Received 09 Nov 2015 Accepted 04 Dec 2015 Published 25 December 2015 A B S T R A C T Stem cell has great therapeutic potentials as it proliferates indefinitely, as well as gives rise to other cell type in our body. However, human pluripotent stem cell (hPSC) technology faces some obstacles associated with tumorigenicity and telomere shortening. And the risk of tumorigenicity of hPSC upon transplantation is one of the major hurdles, which must be overcome before hPSC based clinical practices. Interestingly, human amniotic stem cell (hASC) showed promising results by bypassing from the drawback. But, the important question is how hASC fully or partially escape from the progression of teratoma in severe combined immunodeficiency (SCID) mice, which remains unravel. It is decisive to comprehend the molecular mechanisms responsible for that of teratogenic and this non-teratogenic effect. Evidently, teratoma represents a critical line between stem cells, differentiation and tumorigenesis.
    [Show full text]
  • Amniotic Fluid Stem Cells: a Promising Therapeutic Resource for Cell-Based Regenerative Therapy
    1846 Current Pharmaceutical Design, 2012, 18, 1846-1863 Amniotic Fluid Stem Cells: a Promising Therapeutic Resource for Cell-Based Regenerative Therapy Ivana Antonucci1,2, Andrea Pantalone2,3, Stefano Tetè1,2, Vincenzo Salini2,3, Cesar V. Borlongan4, David Hess5 and Liborio Stuppia1,2* 1Department of Oral Sciences, Nano and Biotechnologies, G. d’Annuzio University, Chieti-Pescara, Italy; 2Stem Tech Group, Aging Research Center (CESI), Chieti, Italy; 3Department of Orthopaedics and Traumatology, G. d'Annunzio University, Chieti-Pescara, Italy; 4Department of Neurosurgery and Brain Repair, University of South Florida College of Medicine, Tampa, FL, USA; 5De- partment of Neurology, Georgia Health Sciences University, Augusta, G, USA Abstract: Stem cells have been proposed as a powerful tool in the treatment of several human diseases, both for their ability to represent a source of new cells to replace those lost due to tissue injuries or degenerative diseases, and for the ability of produce trophic molecules able to minimize damage and promote recovery in the injured tissue. Different cell types, such as embryonic, fetal or adult stem cells, human fetal tissues and genetically engineered cell lines, have been tested for their ability to replace damaged cells and to restore the tis- sue function after transplantation. Amniotic fluid -derived Stem cells (AFS) are considered a novel resource for cell transplantation ther- apy, due to their high renewal capacity, the “in vitro” expression of embryonic cell lineage markers, and the ability to differentiate in tis- sues derived from all the three embryonic layers. Moreover, AFS do not produce teratomas when transplanted into animals and are char- acterized by a low antigenicity, which could represent an advantage for cell transplantation or cell replacement therapy.
    [Show full text]
  • A Paediatric Perspective on Stem Cells: Expression, Function and Clinical Relevance Arnaldo Cantani* Department of Pediatrics, University of Roma “La Sapienza”, Italy
    e Engine ym er z in n g E Cantani, Enz Eng 2016, 5:3 DOI: 10.4172/2329-6674.1000154 Enzyme Engineering ISSN: 2329-6674 ReviewResearch Article Article Open Access A Paediatric Perspective on Stem Cells: Expression, Function and Clinical Relevance Arnaldo Cantani* Department of Pediatrics, University of Roma “La Sapienza”, Italy Abstract In this paper, we provide an overview of the potential advantages and disadvantages of different stem and progenitor cell populations identified to date in amniotic fluid, along with their properties and potential clinical applications. In the last ten years, placenta, fetal membranes (i.e. amnion and chorion), and amniotic fluid have been extensively investigated as a potential non-controversial source of stem cells. They are usually discarded after delivery and are accessible during pregnancy through amniocentesis and chorionic villus sampling. Several populations of cells with multi-lineage differentiation potential and immune-modulatory properties have been isolated from the human placenta and fetal membranes; they have been classified by an international workshop as human amniotic epithelial cells (hAECs) human amniotic mesenchymal stromal cells (hAMSCs) human chorionic mesenchymal stromal cells (hCMSCs) by Igura et al. and Anker et al., and human chorionic trophoblastic cells (hCTCs). Although only recently described, these cells may, given the easier accessibility of the AF in comparison to other extra-embryonic tissues, hold much promise in regenerative medicine. Keywords: Stem cells; Multi-lineage; Amniotic fluid; Gastro-intestine 7th week to 600 ml in the 25th week, 1000 ml in the 34th week and 800 ml at birth. Introduction During the first half of gestation, the AF results from active sodium In the last ten years, placenta, fetal membranes (i.e.
    [Show full text]
  • Amniotic Derived Stem Cells: Role and Function in Regenerative
    ll Scienc Ce e f & o T l h a e n r a r a p p u u y y o o J J Journal of Cell Science & Therapy Larson and Gallicchio, J Cell Sci Ther 2017, 8:3 ISSN: 2157-7013 DOI: 10.4172/2157-7013. 1000269 Research Article Open Access Amniotic Derived Stem Cells: Role and Function in Regenerative Medicine Andrew Larson and Vincent S Gallicchio* Department of Biological Sciences, College of Science, Clemson University, Clemson, USA *Corresponding author: Vincent S Gallicchio, Department of Biological Sciences, College of Science, Clemson University, Clemson, USA, Tel: +1 8646563311; E-mail: [email protected] Rec Date: Apr 11, 2017, Acc Date: May 02, 2017, Pub Date: May 04, 2017 Copyright: © 2017 Larson A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited Abstract Stem cells are undifferentiated biological cells that have the ability to differentiate into any specialized cells capable of cell division through mitosis. Amniotic stem cells (ASCs) are collectively a mixture of stem cells that can be obtained from amniotic fluid and tissue. Amniotic fluid is commonly called a pregnant woman’s water. It is the protective liquid contained within the amniotic sac that lubricates the embryo or fetus that is generated by the maternal blood plasma, which is the liquid component that contains all of the blood derived cells in suspension. The amniotic fluid is absorbed through the fetal tissue and skin until the 20-25th week of pregnancy when the fetal skin begins to keratinize.
    [Show full text]
  • Human Amniotic Epithelial Stem Cells: a Promising Seed Cell for Clinical Applications
    International Journal of Molecular Sciences Review Human Amniotic Epithelial Stem Cells: A Promising Seed Cell for Clinical Applications 1, 2, 1 1, 1, Chen Qiu y, Zhen Ge y, Wenyu Cui , Luyang Yu * and Jinying Li * 1 MOE Laboratory of Biosystems Homeostasis & Protection and College of Life Sciences-iCell Biotechnology Regenerative Biomedicine Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058, China; [email protected] (C.Q.); [email protected] (W.C.) 2 Institute of Materia Medica, Hangzhou Medical College, Hangzhou 310013, China; [email protected] * Correspondence: [email protected] (L.Y.); [email protected] (J.L.) These authors contributed equally. y Received: 31 August 2020; Accepted: 15 October 2020; Published: 19 October 2020 Abstract: Perinatal stem cells have been regarded as an attractive and available cell source for medical research and clinical trials in recent years. Multiple stem cell types have been identified in the human placenta. Recent advances in knowledge on placental stem cells have revealed that human amniotic epithelial stem cells (hAESCs) have obvious advantages and can be used as a novel potential cell source for cellular therapy and clinical application. hAESCs are known to possess stem-cell-like plasticity, immune-privilege, and paracrine properties. In addition, non-tumorigenicity and a lack of ethical concerns are two major advantages compared with embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). All of the characteristics mentioned above and other additional advantages, including easy accessibility and a non-invasive application procedure, make hAESCs a potential ideal cell type for use in both research and regenerative medicine in the near future.
    [Show full text]
  • Stem Cells Powerpoint
    Adipose stem cells Simple liposuction technique Adipose stem cells Aspirate and Aspirate fat and digest wash fat x2 with collagenase for 30 – 60 minutes Surgery SVF (cell pellet) Patient Fat Differentiable Bone ASC Potential clinical Muscle applications Cartilage Culture SVF to obtain ASC Adipose stem cells Bone marrow stem cells Autologous stem cells are only as good as the donor and are subject to the limitations of the individual’s health status and age. Autologous stem cells After the age of 30 the stem cell content of any donor markedly decreases with age. Progressive Depletion of Stem Cell Population with Age Stem Cells decline with age 0.00010 0.00008 0.00006 0.00004 0.00002 MSC/Marrow Cells MSC/Marrow 0.00000 New Born Teen 35 yrs 50 yrs 80 yrs Age Autologous stem cells These cells may be a good choice in the young, but not a good choice for patients of advancing age. Yet many of stem cell clinics in the US STILL utilize autologous stem cells ONLY. Stem Cells 101 Course Objectives are now half completed and are as follows: 1.You have now been made aware of the stem cell industry and how it works. 2.You are now educated and empowered with the ability to sort through the pitfalls of autologous stem cell therapy. 3.You are rapidly losing your VULNERABILITY. 4.Now let’s move on to the second half of Stem Cells 101. Heterologous stem cells These are cells that come from one human being and are given to another. Heterologous stem cells These are cells derived from babies delivered by scheduled C-section in mothers that have been screened for health issues.
    [Show full text]