University of Patras Department of Pharmacy

ECTS

UNIVERSITY OF PATRAS

DEPARTMENT OF PHARMACY

INFORMATION PACKAGE 2010-11

1 GENERAL INFORMATION AND STRUCTURE OF THE DEPARTMENT The Department The Department of Pharmacy of the University of Patras was founded in 1977. Its first undergraduate students were admitted in 1978. The departmental graduate program, having been in effect since 1994, was revised in 2001 and leads to Graduate Specialization Diplomas in the areas of: Industrial Pharmaceutics and Drug Analysis, Pharmaceutical Chemistry-Natural Products: Design, Synthesis and Analysis of Bioactive Compounds, Molecular Pharmacology – Clinical Pharmacy, Pharmaceutical Biotechnology and Biomedicine, Pharmaceutical Marketing and/or to Doctoral degree.

Since its foundation the Department has been committed to teaching and research of international standards. During the previous academic years the Department was awarded several grants through competitive programs (EPEAEK) financed by the Ministry of Education for the improvement of its academic activities. From the EPEAEK among others were financed the reforming of the undergraduate program and the introduction of the Pharmaceutical Practice through study visits of the senior undergraduate students to pharmaceutical industries. The departmental “Continuous Education” program was also financed as well as the initiation of three more Graduate Specialization Diploma namely the “Medicinal Chemistry”, in co-operation with the Chemisty Department and the Medical School of the University of Patras, the “Isolation and Synthesis of Bioactive Natural Products” in co-operation with the Chemistry Departments of the Universities of Crete, Patras, Athens, Thessaloniki and Ioannina anf the “Bioinformatics in Life Sciences” in co-operation with the Departments of Medicine and Biology.

There are currently 350 students in total following undergraduate studies. Each year the Department accepts 130 fresh undergraduate students and 30 graduate students. The Department has 23 faculty members who supervise the research of about 70 graduate students. The Department of Pharmacy consists of seven laboratories. The ruling bodies in the department include the chairman, the deputy chairman and the general assembly.

Chairman: Professor Paul Kordopatis, Tel. (+30 2610) 969 934 e-mail: [email protected]

Deputy Chairman: Professor Christos Kontoyannis, Tel. (+30 2610) 997 727 e-mail: [email protected] Registar: Mrs.Zoi Kanellopoulou, Tel. (+30 2610) 997 537

The ECTS Departmental Coordinator: Assistant Professor Konstantinos Poulas Tel.: (+30 2610) 969 953 Fax : (+30 2610) 969 955 e-mail: [email protected]

2 STRUCTURE OF THE DEPARTMENT

Laboratories A. Laboratory of Pharmaceutical Chemistry B. Laboratory of Pharmaceutical Technology C. Laboratory of Physical Pharmacy D. Laboratory of Pharmacokinetics E. Laboratory of Instrumental Pharmaceutical Analysis F. Laboratory of Pharmacognocy & Chemistry of Natural Products G. Laboratory of Molecular Pharmacology H. Laboratory of Molecular Biology and Immunology

List of Faculty Members of the Department Professors: S. Antimisiaris (Ph.D., University of Athens, Greece) C. Camoutsis (Ph.D., University of Athens, Greece) P. Cordopatis (Ph.D., University of Patras, Habilitation, University of Patras, Greece) C. Kontoyannis (Ph.D., Georgetown University, U.S.A) A. Papapetropoulos (Ph.D., Medical College of Georgia, USA) A. Tsarbopoulos (Ph.D., Michigan State University, USA) S. Tzartos (Ph.D., Univ. of Athens, Greece)

Associate Professors: K. Avgoustakis (Ph.D., Chelsea College, London University, United Kingdom) S. Nikolaropoulos (Ph.D., University of Patras, Greece) P. Magriotis (Ph.D., SUNY at Stony Brook, USA) E. Papadimitriou (Ph.D., University of Patras, Greece) G. Sivolapenko (Ph.D., University of London, UK) G. Sotiropoulou (Ph.D., University of Thessaloniki, Greece) G. Spyroulias (Ph.D., University of Crete)

Assistant Professors: P. Klepetsanis (Ph.D., University of Patras, Greece) Q. F. Lamari (Ph.D., University of Patras, Greece) V. Magafa (Ph.D., University of Patras, Greece) G. Pairas (Ph.D., University of Patras, Greece) G. Patrinos (Ph.D., University of Athens, Greece) K. Poulas (Ph.D., University of Athens, Greece) S. Topouzis (Louis Pasteur University / Strasbourg I, France)

Lecturers: M. Orkoula (Ph.D., University of Patras, Greece) E. Fousteris (Ph.D, University of Patras, Greece)

3 B. Research Activities of the Faculty Members

Sophia G. Antimisiaris Phone: + 30 2610 997725 e-mail: [email protected]

Professor Ph.D. University of Athens

Current Focus of Research Biopharmaceutics and Pharmacokinetics: Improving Drug Biodistribution and Pharmacokinetics through Advances Drug Delivery System Design Nanomedicines: Liposomal or Nanoparticulate systems for Controlled Drug (or Vaccine)Delivery and/or Targeting. Application of novel lipid vesicles (Arsonoliposomes) in Anticancer / Antiparasitic Therapeutics. Novel Controlled-(Release)-Drug-Releasing Stents. Delivery of Microbicides for prevention of Sexually transmitted HIV. Ocular Drug Delivery (intravitreal injection of novel drug delivery systems). Drug Delivery Systems for targeting alveolar macrophages after delivery by nebulization Liposomal Drugs -in- Polymeric Films for sustained drug release and drug protection. Application of Liposomes in Analytical Technigues

Selected Publications

G. Koromila, G.Michanetzis, Y.F. Missirlis,S.G.Antimisiaris*, Heparin incorporating liposomes as a delivery system of heparin from PET - covered metallic stents: Effect on haemocompatibility, Biomaterials, 27:12, 2525-2533, 2006

M. Zaru, S. Mourtas, P. Klepetsanis, A.M. Fadda and S.G.Antimisiaris*, Liposomes for drug delivery to the lungs after nebulization. Eur. J. Pharmaceutics & Biopharmaceutics, 67 (3), pp. 655-666, 2007

M.L. Manca , S. Mourtas, V. Drakopoulos, A M. Fadda, S G.Antimisiaris*, PLGA, Chitosan or Chitosan-coated PLGA Microparticles for Alveolar Delivery? A comparative study of particle stability during nebulization. Colloids and Surfaces B: Biointerfaces, 62 (2), pp. 220-231, 2007

S. Mourtas, M. Haikou, M. Theodoropoulou, C. Tsakiroglou and S. G. Antimisiaris*, How liposome type, composition and loading concentration influence the rheological properties of a liposomal gel. J. Colloid & Inter. Scienc B: Biointerfaces, 317 (2), pp. 611-619, 2008

C. Peptu, M. Popa and S.G. Antimisiaris*, Release of Liposome-Encapsulated Calcein from Liposome Entrapping Gelatin-Carboxymethylcellulose Films: A Presentation of Different Possibilities. J. Nanoscience & Nanotechnology, 8, 2249– 2258 2008

4 Konstantinos Avgoustakis Phone: + 30 2610 997726 e-mail: [email protected]

Associate Professor Ph.D. University of Thessaloniki Ph.D University of London

Current Focus of Research Synthesis, characterization and medical applications of Biodegradable and Biocompatible polymers

Preparation, physicochemical study and medical applications of polymeric micro/nano-particles and liposomes.

Selected Publications

Z.Panagi, K.Avgoustakis, G. Evangelatos and D.S.Ithakissios in "Protein induced CF release kinetics from liposomes in vitro and its correlation with the BLOOD/RES biodistribution of liposomes" Int. J. Pharm., 163 (1998) 103-114.

Z.Panagi, K.Avgoustakis, G. Evangelatos and D.S.Ithakissios in "In vitro binding of serum proteins on liposomes and its correlation with the in vivo BLOOD/RES ratio of liposomes. Int. J. Pharm., 176(1999) 203-207.

A.Beletsi, L.Leontiadis, P.Klepetsanis, D.S.Ithakissios and K.Avgoustakis in "Effect of preparative variables on the properties of PLGA-mPEG copolymers related to their application in controlled drug delivery. Int. J. Pharm., 182(1999) 187-197.

Z.Panagi, A.Beletsi, G.Evangelatos, P.Klepetsanis, D.S.Ithakissios and K. Avgoustakis in "Biodistribution of PLGA-mPEG nanoparticles: Effect of copolymer composition". Proc. Int. Symp. Controll. Release Bioact. Mater., Controlled Release Soc., 27, 318.

Charalambos Camoutsis Phone: + 30 610 997659 e-mail: [email protected]

Professor Ph.D. University of Athens

Current Focus of Research Heterocyclic steroids and Heterocyclic aromatic compounds. Ηomo-azasteroidal esters of carboxylic derivatives of N,N-bis(2-chloroethyl) aniline. Antitumor Activity.

Selected Publications

K. Psaraki, N.A. Demopoulos, G. Stephanou and Ch. Camoutsis, "m-N,N-bis(2- chloroethyl)aminocinnamic acid and four new homo-aza-steroidal esters induce chromosomal abnormalities and affect protein synthesis in human lymphocytes in vitro", Anti-Cancer Drugs, 8, (1997) 73.

T. Mavromoustakos, I.K. Stamos, Ch. Camoutsis, E. Theodoropoulou, M. Zervou and E. Humfer, "Nuclear magnetic resonance spectral analysis and conformational properties of 11-benzoyl-9,9a,10,11-tetrahydro[4,3-ab]carbazole", Journal of Pharmaceutical and Biomedical Analysis, 16 (19980 723.

Ch. Camoutsis and S. Nikolaropoulos, "Steroidal Isoxazoles, Isoxazolines and Isoxazolidines", Journal of Heterocyclic Chemistry, 35 (1998) 731.

Ch. Camoutsis, C. Sambani, D.T.P. Trafalis and P. Peristeris, "On the formation of steroidal amindoesters of 4-[N,N-bis(2-chloroethyl)amino]benzoic acid and their cytotoxic activity", European Journal of Medicinal Chemistry, 34 (1999) 645.

C.D. Xenos and Ch. Camoutsis, "Synthesis of A- and D- Homoazasteroidal- isoxazoles", Journal of Heterocyclic Chemistry 36, (1999) 1343.

Paul Cordopatis Phone: +30 2610 997721/713 Fax: +30 2610 997714 e-mail: [email protected]

Professor Ph.D., University of Patras (1976) Habilitation in Organic Chemistry, University of Patras (1981)

Current Focus of Research Design, synthesis, conformational analysis and pharmacological activities of bioactive peptides. Structure-Activity relationships. Implication for the synthesis of therapeutical useful compounds.

Selected Publications

Vamvakas S-S.M., Leondiadis L., Pairas G., Manessi-Zoupa E., Spyroulias G.A., Cordopatis P. Expression, purification, and physicochemical characterization of the terminal N-active site of human angiotensin-I converting enzyme. J. Pept. Sci. (2006) “in press”.

Spyroulias GA, Galanis AS, Petrou Ch, Vahliotis D, Sotiriou P, Nikolopoulou A, Nock B, Maina T, Cordopatis P. 3D solution structure of [Tyr3]octreotate derivatives in DMSO: structure differentiation of peptide core due to chelate group attachment and biologically active conformation. Med Chem. 1(5): 487-99 (2005).

Zompra AA, Magafa V, Lamari FN, Nikolopoulou A, Nock B, Maina T, Spyroulias GA, Karamanos NK, Cordopatis P. GnRH analogues containing conformationally restricted amino acids in positions 3 and 6: Differential impact on pituitary binding affinity and direct antiproliferative effect on breast cancer cells J. Peptide Res. 66 (s1): 57-64 (2006).

Nikolopoulou A, Maina T, Sotiriou P, Cordopatis P, Nock BA. Tetraamine-modified octreotide and octreotate: labeling with 99mTc and preclinical comparison in AR4-2J cells and AR4-2J tumor-bearing mice. J Pept Sci. 12(2):124-31 (2006).

Galanis AS, Spyroulias GA, Pairas G, Manessi-Zoupa E, Cordopatis P. Solid-phase synthesis and conformational properties of angiotensin converting enzyme catalytic- site peptides: the basis for a structural study on the enzyme-substrate interaction. Biopolymers 76(6): 512-26 (2004).

7 Manolis Fousteris Phone: +30 2610 969391 Fax: +30 2610 992776 e-mail: [email protected]

Lecturer Ph.D., University of Patras (2005)

Current Focus of Research 1. Design and synthesis of small heterocyclic molecules as potential inhibitors of protein kinases. 2. Design, synthesis and evaluation of the biological activity of new steroidal alkylating agents.

Recent publications 1. Koutsourea A. I.; Fousteris M. A.; Arsenou E. S.; Papageorgiou A.; Pairas G. N.; Nikolaropoulos S. S. Rational design, synthesis, and in vivo evaluation of the antileukemic activity of six new alkylating steroidal esters. Bioorg. Med. Chem. 16, 5207-5215 (2008).

2. Fousteris M. A.; Papakyriakou A.; Koutsourea A.; Manioudaki M.; Lampropoulou E.; Papadimitriou E.; Spyroulias G. A.; Nikolaropoulos S. S. Pyrrolo[2,3-a]carbazoles as Potential Cyclin Dependent Kinase 1 (CDK1) Inhibitors. Synthesis, Biological Evaluation, and Binding Mode through Docking Simulations. J. Med. Chem. 51, 1048-1052 (2008).

3. Fousteris M. A.; Koutsourea A. I.; Arsenou E. S.; Papageorgiou A.; Mourelatos D. Nikolaropoulos S. S. Structure-antileukemic activity relationship study of B- and D- ring modified and nonmodified steroidal esters of 4-methyl-3-N,N-bis(2- chloroethyl)amino benzoic acid: a comparative study. Anti-Cancer Drugs, 18, 997- 1004 (2007).

Pavlos Klepetsanis Phone: + 30 2610 997712 Fax: + 30 2610 996302 e-mail: [email protected]

Assistant Professor Ph.D. University of Patras, Greece

Current Focus of Research Study of cyclodextrin/drug inclusion compounds formation. Solubilization of water isoluble drugs Physicochemical characterisation of pharmaceutical forms. Biomineralization and Demineralization. Physicochemical study of Biopolymers. Corrosion of metallic implants in biological fluids.

Selected Publications

Zaru M., Mourtas S., Klepetsanis P., Fadda A.M. and Antimisiaris S.G., „Liposomes for drug delivery to the lungs by nebulization‟, European Journal of Pharmaceutical Sciences, 67(3), 2007, p. 655-666.

Blin F., Koutsoukos P.G. Klepetsanis P. and Forsyth M., „The corrosion inhibition mechanism of new rare earth cinnamate compounds – Electrochemical studies‟, Electrochimica Acta, 52(21), 2007, p.6212-6220.

Zagana P., Haikou M., Klepetsanis P., Giannopoulou E., Ioannou P.V. and Antimisiaris S.G., „In vivo distribution of arsonoliposomes: Effect of vesicle lipid composition‟, International Journal of Pharmaceutics, 347(1-2), 2008, p.86-92.

Lioliou M.G., Kofina A.N., Paraskeva C.A., Klepetsanis P.G., Ostvold T., Payatakes A.C. and Koutsoukos P.G., „Controlled precipitation of sparingly soluble phosphate salts using enzymes. I. Controlled development of solution supersaturation in situ‟, Crystal Growth & Design, 8(4), 2008, p.1390-1398.

Beletsi A., Klepetsanis P., Ithakissios D.S., Kounias S, Stavropoulos A. and Avgoustakis K., „Simultaneous optimization of cisplatin-loaded PLGA-mPEG nanoparticles with regard to their size and drug encapsulation‟, Current Nanoscience, 4(2), 2008, p.173-178.

Mourtas S., Aggelopoulos A.C., Klepetsanis P., Tsakiroglou D.C. and Antimisiaris S.G., „Complex hydrogel systems composed of Polymers, Liposomes and Cyclodextrins : Implications of composition on Rheological Properties and Aging‟, Langmuir, 25(15), 2009, p.8480-8488.

Christos Kontoyannis Phone: + 30 2610 997727 Fax: +30 2610 997658 e-mail: [email protected]

Professor Ph.D. Georgetown University, USA

Current Focus of Research Development of non-destructive analytical methods using spectroscopic (Raman, IR, XRF) and electrochemical techniques (DPP, Impedance spectroscopy, CV). Applications in bio-ceramics, drugs, urinary stones, bones, liposomes, polymers etc. Development of new biomaterials (synthesis, analysis, physicochemical properties).

Selected Publications

I. Karabas, M.G. Orkoula, C.G. Kontoyannis, “Analysis and Stability of Polymorphs in Tablets: The Case of Risperidone”, Talanta, 71 (2007) 1382.

M. Orkoula, C. Kontoyannis, C. Markopoulou and J.E. Koundourellis, “Validation οf a Direct, Non-Destructive Quantitative Analysis, of Amiodarone Hydrochloride in Angoron® Formulations Using FT-Raman Spectroscopy”, Talanta, 73 (2007) 258.

D. Skorda and C. Kontoyannis, "Identification and Quantitative Determination of Atorvastatin Calcium Polymorph in Tablets Using FT-Raman Spectroscopy” Talanta 74 (2008) 1066.

C. Markopoulou and J.E. Koundourellis, M. Orkoula, C. Kontoyannis, “Quantitative non destructive methods for the determination of ticlopidine in tablets using reflectance NIR and FT-Raman spectroscopy”, Applied Spectroscopy, 62 (2008) 251.

M. Orkoula, C. Kontoyannis, “Non-Destructive Quantitative Analysis of Risperidone in Film-Coated Tablets” J. Pharm. Biomed. Anal., 47 (2008) 631

Fotini N. Lamari Phone: +30 2610 969335/713 Fax: +30 2610 997714 e-mail: [email protected]

Assistant Professor Ph.D., University of Patras (2000)

Current Focus of Research Medicinal and Aromatic plants-Natural Products (expertise in Crocus genus and glycosaminoglycans) Isolation and structural characterization with emphasis on polysaccharides: Structure- function studies of biomolecules Development of analytical methods (capillary electrophoresis, HPLC) for determination of natural products in biologic samples In vitro and in vivo evaluation of biologic properties with emphasis on the antioxidant potential

Selected Publications Effect of Polyphenol Rich Blueberry Extract on Cognitive Performance of Mice, on Brain Antioxidant Markers and Acetylcholinesterase Activity. M. A. Papandreou, A. Dimakopoulou, Z.I. Linardaki, P. Cordopatis, D. Klimis-Zacas, M. Margarity, and F.N. Lamari. Behav Brain Res. (2009) 198(2): 352-8.

Inhibitory activity on amyloid-β aggregation and antioxidant properties of Crocus sativus stigmas extract and its crocin constituents. Papandreou M.A, Kanakis C.D., Polissiou M.G., Efthimiopoulos S., Cordopatis P., Margarity M., & Lamari F.N. J. Agric. Food Chem. (2006) 54: 8762

Inhibition of Breast Cancer Cell Proliferation by Style Constituents of Different Crocus Species. Chryssanthi D.G., Lamari F.N., Iatrou G., Pylara A., Karamanos N.K., Cordopatis P.Anticancer Res. (2007) 27(1A): 357-362

Identification of oligomeric domains within dermatan sulfate chains using differential enzymic treatments, derivatization with 2-aminoacridone and capillary electrophoresis.T. N. Mitropoulou,F. Lamari , A. Syrokou, A. Hjepre, N. K. Karamanos. Electrophoresis 22 (2001) 2458-2463.

Structure of chondroitin sulfate. F.N. Lamari and N.K. Karamanos. In volume 53 entitled «Chondroitin Sulfate: Structure, Role and Pharmacological Activity» of the book series "Advances in Pharmacology" (editor: N. Volpi), Elsevier, 2005, pp. 33- 48.

Vassiliki Magafa Phone: + 30 2610 997713/969933 Fax: + 30 2610 997714 e-mail: [email protected]

Assistant Professor Ph.D. University of Patras

Current Focus of Research Chemistry of amino acids and peptides Synthesis in liquid and solid phase analogues of biologically important peptides Study of structure-activity relationships of biologically active peptides Synthesis and study of Cysteine - Containing Oligopeptides and their Complexes with heavy metals

Selected Publications

Interaction of Hg (II) with Tetrapeptides containing Cysteinyl and Histidinyl Residues. V. Magafa, G. Stavropoulos, P. Tsiveriotis and N. Hadjiliadis Inorg. Chim. Acta, (1998), 272, 7-17

Substance P C-Terminal Octapeptide Analogues Augment Tumor Necrosis Factor-α Release by Human Blood Monocytes and Macrophages Wen-Zhe Ho, G. Stavropoulos, Jian-Ping Lai, Bao-Feng Hu, V. Magafa, S.Anagnostidis and SD.Daglas Journal of Neuroimmunology, (1998), 82, 126-132

"Synthesis and Biological Evaluation of Oxytocin Analogues Containing L-α-t- butylglycine [Gly(But)] in Positions 8 or 9". M., Fragiadaki, V. Μagafa, J. Slaninová and P. Cordopatis Peptides, (2003), 24, 1425-1431

"2-Amino-4-pyrrolidinothieno[2,3-d]pyrimidine-6-carboxylic Acid as an N-Terminal Surrogate in Amino Acid and Peptide Analogues". E. Bissyris, D. Belekos,V. Magafa, P. G. Tsoungas, G. Varvounis and P. Cordopatis Synthesis, (2005), 18, 3159-3166

"Synthesis and Biological Activity of Oxytocin Analogues Containing Conformationally Restricted Residues in Position 7". M. Fragiadaki, V. Magafa, L. Borovickova, J. Slaninová and P. Cordopatis. European Journal of Medicinal Chemistry, (2007), 42, 799-806

12 Platon Magriotis Phone: + 30 2610 997659 e-mail: [email protected]

Associate Professor Ph.D. SUNY at Stony Brook

Current Focus of Research Synthesis of molecules with biological activity Synthesis anti-cancer antibiotic natural products

Synthesis of biologically and pharmacologically relevant molecules such as a- and b- amino acids, b-lactams, piperidines, and piperazines

Total Synthesis of antitumor, antibiotic Natural Products (Cyclostreptin-(-)- FR182877, Ecteinascidin 743, Saframycin A, and Abyssomicin C).

New Synthetic Methodology including novel routes to b-lactams and tetrasubstituted olefins (Tamoxifen analogs).

Catalytic Enantioselective Synthesis of Substituted Piperazines as a general approach for the Construction of the Tetrahydroisoquinoline Antitumor Antibiotics.

Selected Publications

1. Yiotakis A.; Magriotis, P. A.; Vassiliou, S. “A Simple Synthesis of the Metabotropic Receptor Ligand (2S)-a-(Hydroxyymethyl)- glutamic acid and its Fmoc Protected Derivatives”. Tetrahedron: Asymmetr. 18, 873-877 (2007).

2. Vassiliou, S.; Yiotakis, A.; Magriotis, P. A. “Efficient Enantioselective Synthesis of Orthogonally Protected (R)-a-Alkylserines Compatible with the Solid Phase Peptide Synthesis”. Tetrahedron Lett. 47, 7339-7341 (2006).

3. Vassiliou, S.; Magriotis, P. A. “Improved Schöllkopf Construction of Quaternary a-Amino Acids: Efficient Enantioselective Synthesis of Integrin LFA-1 Antagonist BIRT-377”. Tetrahedron: Asymmetr. 17, 1754-1757 (2006).

13 Sotiris Nikolaropoulos Phone: + 30 2610 997723 e-mail: [email protected]

Associate Professor Ph.D. University of Patras

Current Focus of Research Design and synthesis of heterocyclic steroids Design, synthesis and pharmacological evaluation of potential antitumor agents Measurements of physicochemical parameters via simple HPLC instrumentation QSAR

Selected Publications

S.Nikolaropoulos, E.S.Arsenou, A.Papageorgiou and D.Mourelatos: Antitumor and Cytogenetic Effects of Esteric (ASE) and Amidic (ASA) Steroidal Derivative of p- bis(2-Chloroethyl)amino phenylacetic Acid (CAPA). A Comparative Study Anticancer Research, 1997, 17, 4525-4530.

Ch. Camoutsis and S.Nikolaropoulos: Steroidal isoxazoles, isoxazolines and isoxazolidines J. Heterocyclic Chem., 1998, 35(4), 731-759.

A.Papageorgiou, S.Nikolaropoulos, E.S Arsenou, E.Karaberis, D.Mourelatos, A.Kotsis, E.Chryssogelou: Synergistic cytogenetic and antineoplastic effects by two esteric steroidal derivatives of nitrogen mustards Chemotherapy, 1999, 45, 61-67.

E. Arvanitopoulou, S. Nikolaropoulos, F. Bazoti and N.A. Katsanos: Physicochemical measurements in liquids by simple HPLC instrumentation, Journal of Liquid Chromatography 2000.

S.Nikolaropoulos, D.Tsavdaridis, E.Arsenou, A.Papageorgiou, E.Karaberis and D.Mourelatos: Synergistic Antineoplastic and Cytogenetic Effects by the Combined Action of Two Homo-aza-Steroidal Esters of Nitrogen Mustards on P388 and L1210 Leukaemias in Vivo and in Vitro, Anticancer Research, 2000.

14 Malvina Orkoula Phone: + 30 2610 969941 Fax: +30 2610 997658 e-mail: [email protected]

Lecturer Ph.D. University of Patras, Greece

Current Focus of Research Instrumental analysis of solids and liquids. Non-destructive techniques. Application in pharmaceutical formulations. Development of novel non-destructive, non-invasive methodologies for the study of bone and bone diseases. Physical chemistry of aquatic solutions, colloidal systems, chemistry of interfaces. Dissolution, crystallization of open systems and inhibition. Preparation and characterization of polymorphs. Wetting of solids.

Selected Publications

C.G. Kontoyannis and M.G. Orkoula, «Quantitative Determination of the Cubic, Tetragonal and Monoclinic Phases in Partially Stabilized Zirconias by Raman Spectroscopy», J. Materials Science, 29 (1994) 5316-5320.

C.G. Kontoyannis and M.G. Orkoula, «Quantitative Non-Destructive Determination of Salicylic Acid Acetate in Aspirin Tablets by Raman Spectroscopy», Talanta, 41, 11 (1994) 1981-1984.

M.G. Orkoula, C.G. Kontoyannis, C.K. Markopoulou and J.E. Koundourellis, «Development of Methodologies Based on HPLC and Raman Spectroscopy for Monitoring the Stability of Lovastatin in Solid State in the Presence of Gallic Acid», Journal of Pharmaceutical and Biomedical Analysis, 35 (2004) 1011-1016.

M.G. Orkoula, C.G. Kontoyannis, C.K. Markopoulou and J.E. Koundourellis, «Validation of a Direct Non-Destructive Quantitative Analysis of Amiodarone Hydrochloride in Angoron® Formulations Using FT-Raman Spectroscopy», Talanta, 73 (2007) 258-261

I. Karabas, M.G. Orkoula and C.G. Kontoyannis, «Analysis and Stability of Polymorphs in Tablets: The Case of Risperidone», Talanta, 71 (2007) 1382-1386.

C. K. Markopoulou, J.E. Koundourellis, M.G. Orkoula and C. G. Kontoyannis, «Quantitative Non-Destructive Methods for the Determination of Ticlopidine in Tablets Using Reflectance Near-Infrared and Fourier-Transform Raman Spectroscopy», Applied Spectroscopy, 62 (2008) 251-257.

George Pairas Phone: + 30 2610 997724 e-mail: [email protected]

Assistant Professor Ph.D. University of Patras

Current Focus of Research Synthesis of ester and amide derivatives of anticancer agents with modified steroids. Computer aided design - synthesis of new compounds with biological activity.

Selected Publications

Galanis, A., Spyroulias, A. G., Pierattelli, R., Tzakos, A., Troganis, A., Gerothanassis, I.,Pairas, G. , Manessi-Zoupa, E. and Cordopatis, P., “Zinc-Binding in Peptide Models of ACE Active Sites Studied Through 1H NMR and Chemical Shift Perturbation Mapping”, Biopolymers, 69, 244 (2003)

A. Galanis, G. Spyroulias,G. Pairas , E. Manessi-Zoupa and P. Cordopatis, "Solid Phase Synthesis and Conformational Properties of ACE Catalytic Site Peptides: The Basis for a Structural Study on the Enzyme-Substrate Interaction". Biopolymers, 76, 512 (2004)

S. Vamvakas, L. Leondiadis,G. Pairas , E. Manessi-Zoupa, G. Spyroulias and P. Cordopatis, "Expression, Purification and Physicochemical Characterization of N- Terminal Active Site of Human Angiotensin Converting Enzyme (ACE)", Journal of Peptide Science, 13, 31 (2007).

G. Paloumbis, C. Petrou, B. Nock, T. Maina,G. Pairas , P. Tsougas and P. Cordopatis, "Synthesis of a Protected δ-Glycoamino Acid Building Block for Incorporation into Peptide Chains", Synthesis,6, 845 (2007).

Evangelia Papadimitriou Phone: + 30 2610 969336 e-mail: [email protected]

Associate Professor Ph.D. University of Patras, Greece

Current Focus of Research Angiogenesis and tumor growth in vivo and in vitro. Pharmacological studies on the mechanisms involved, mainly concerning the extracellular matrix, free radicals and growth factors. Biological actions of HARP, a growth factor with high affinity for heparin. Effects on endothelial cells, angiogenesis and tumor growth. Mechanisms of action and identification of the receptor. Structure-function studies. Evaluation of new compounds for their antiangiogenic and anti-tumor activity.

Selected publications

Mikelis C., Sfaelou E., Koutsioumpa M, Kieffer N. and Papadimitriou E. “Integrin ανβ3 is a pleiotrophin receptor required for pleiotrophin-induced endothelial cell migration through receptor protein tyrosine phosphatase β/δ”. FASEB J., 23: 1459- 1469, 2009.

Polytarchou C., Hatziapostolou M., Poimenidi E., Mikelis C., Papadopoulou A., Parthymou A. and Papadimitriou E. “Nitric oxide stimulates migration of human endothelial and prostate cancer cells through up-regulation of pleiotrophin expression and its receptor protein tyrosine phosphatase β/δ”. Int. J. Cancer, 124: 1785-1793, 2009.

Hatziapostolou M., Polytarchou C., Katsoris P., Courty J. and Papadimitriou E. “Heparin affin regulatory peptide/pleiotrophin mediates fibroblast growth factor 2 stimulatory effects on human prostate cancer cells”. J. Biol. Chem., 281:32217-32226, 2006.

Hatziapostolou M., Delbe J., Katsoris P., Polytarchou C., Courty J. and Papadimitriou E. “Heparin Affin Regulatory Peptide is a key player in prostate cancer cell growth and angiogenicity”. Prostate, 65: 151-158, 2005.

Polykratis A., Katsoris P., Courty J. and Papadimitriou E. “Characterization of heparin affin regulatory peptide signaling in human endothelial cells”. J. Biol. Chem., 280: 22454-22461, 2005.

Polytarchou C., Hatziapostolou M. and Papadimitriou E. “Hydrogen peroxide stimulates proliferation and migration of human prostate cancer cells through activation of activator protein-1 and up-regulation of the heparin affin regulatory peptide gene”. J. Biol. Chem., 280: 40428-40435, 2005.

17 Andreas Papapetropoulos Phone: + 30 2610 996300 e-mail: [email protected]

Professor Ph.D. Medical College of Georgia, USA

Current Focus of Research Vascular biology Signaling of endothelial-selective tyrosine kinase receptors Protein-protein interactions as drug targets

Selected Publications

Fulton, D., J.P. Gratton, T.J. McCabe, J. Fontana, Y. Fujio, K.Walsh, T.F. Franke, A. Papapetropoulos and W.C. Sessa. Regulation of endothelium-derived nitric oxide production by Akt. Nature 399:597-601, 1999

A. Papapetropoulos, D. Fulton, K. Mahboubi, D. O‟ Connor, F. Li, D.C. Altieri and W.C. Sessa. Angiopoietin-1 inhibits endothelial cell apoptosis via Akt/survivin expression. J. Biol. Chem..275:9102-9105, 2000

Z. Zhou, S. Gross, C. Roussos, S Meurer, W. Müller-Esterl and A. Papapetropoulos. Structural and functional characterization of the dimerization region of soluble guanylyl cyclase. J. Biol. Chem. 279:24935-24943, 2004

Papapetropoulos A, Zhou Z, Gerassimou C, Yetik G, Venema RC, Roussos C, Sessa WC, and Catravas JD. Interaction between hsp90 and soluble guanylyl cyclase: physiological significance and mapping of the domains mediating binding. Mol Pharmacol. 68:1133-41, 2005.

A Pyriochou, D. Beis, V. Koika, C. Potytarchou, E. Papadimitriou, Z. Zhou, A. Papapetropoulos. Soluble guanylyl cyclase activation promotes angiogenesis. J. Pharmacol. Exp. Ther 319:663-71, 2006

D.C. Simoes, T. Vassilakopoulos, D. Toumpanakis, K. Petrochilou, C. Roussos, A. Papapetropoulos. Angiopoietin-1 Protects Against Airway Inflammation and Hyperreactivity in Asthma. Am J Respir Crit Care Med. 177:1314-21, 2008

Z. Zhou, N. Sayed, A. Pyriochou, C. Roussos, D. Fulton, A. Beuve, A Papapetropoulos. Protein Kinase G Phosphorylates Soluble Guanylyl Cyclase on Serine 64 and Inhibits Its Activity. Arterioscler Thromb Vasc Biol. 28:1803-10, 2008

18 Konstantinos Poulas Phone: +30 2610 969953 E-mail: [email protected],

Assistant Professor Ph.D. Univ. of Athens, Greece

Current Focus of Research Monoclonal antibodies against Acetylcholine Receptor X-Ray Crystallographic analysis of proteins Construction, expression and purification of recombinant protein fragments Proteolytic enzymes for myasthenia gravis therapy Epidemiology of myasthenia gravis

Selected Publications

Kalamida D, Poulas K, , Avramopoulou V, Fostieri E, Lagoumintzis G, Lazaridis K, Sideri A, Tzartos S. (2007) Muscle and Neuronal Nicotinic Acetylcholine Receptors: Structure, Function and Pathogenicity. FEBS Journal, 274(15):3799-845

Sideris S, Lagoumintzis G, Kordas G, Kostelidou K, Sotiriadis A, Poulas K, and Tzartos SJ. (2007) Isolation and functional characterization of anti-acetylcholine receptor subunit-specific autoantibodies from myasthenic patients: receptor loss in cell culture. Journal of Neuroimmunology, 189(1-2):111-7

Konstantakaki M, Tzartos S, Poulas K, , Eliopoulos E (2007) Molecular modeling of the complex between Torpedo acetylcholine receptor and anti-MIR Fab198. BBRC 356 (569-575)

Poulas K, Tzartos SJ. (2001) "The 'gender gap' in autoimmune diseases". The Lancet, 357(9251):234.

Poulas K, Eliopoulos E, Vatzaki E, Navaza J, Kontou M, Oikonomakos N, Acharya KR, Tzartos SJ. (2001) "Crystal structure of Fab198, an efficient protector of acetylcholine receptor against myasthenogenic antibodies". European Journal of Biochemistry, 268, 3685-3693.

19 Gregory Sivolapenko Phone: +30 2610 969816 Fax: +30 2610 996302 e-mail: [email protected]

Associate Professor Ph.D. in Medicine, Royal Postgraduate Medical School, University of London, UK

Current Focus of Research Design of novel anticancer and antiinflammatory agents Preclinical and clinical studies of bioavailability and pharmacokinetics of new chemical entities Drug clinical development, design & contact of Phase I-IV clinical trials

Selected Publications

J.S. Stewart, G.B. Sivolapenko, V.Hird, K.A. Davies, M. Wallport, M.A. Ritter and A.A. Epenetos (1990). The clearance of iodine-131 labelled murine mononclonal antibody from patients‟ blood by intravenous human anti-murine immunoglobulin antibody. Cancer Res., 50: 563-567.

K.A.A. Davies, V. Hird, S. Stewart, G.B. Sivolapenko, P. Jose, A.A. Epenetos and M.J. Walport (1990). A study of in vivo immune complex formation and clearance in man. J. Immunol. , 144: 4613-4620.

C. Schatten, N. Pateisky, N. Vavra, P. Ehrenbock, P. Angelberger, G.B. Sivolapenko, and A.A. Epenetos (1991). Lymphposcintigraphy with 123I-labelled epidermal growth factor. Lancet, 337: 295-396.

G.B. Sivolapenko, C. Moreno, W. Smith, J. Corvalan, M.A. Ritter and A.A. Epenetos (1991). Xenogeneic monoclonal antibodies in the management of cancer: control of their in vivo immunogenicity and induction of specific unresponsiveness using an antibody-drug immunoconjugate. Br. J. Cancer, 64: 281-287.

G.B. Sivolapenko, V. Douli, D. Pectasides, D. Skarlos, G. Sirmalis, R. Hussein, J. Cook, N.S. Courtenay-Luck, E. Merkouri, K. Konstantinides and A.A. Epenetos (1995). Breast cancer imaging with radiolabelled peptide from complementarity- determining region of antitumour antibody. Lancet, 346: 1662-1666.

20 Georgia Sotiropoulou Phone: + 30 2610 969939 FAX: + 30 2610 969940 E-mail: [email protected]

Associate Professor Ph.D. in Biochemistry, Aristotle University of Thessaloniki, Greece

Current Focus of Research Cancer invasion and metastasis: molecular mechanisms, diagnostic and drug targets. Tumor suppressor genes and proteins. Differential gene expression in cancer: methodologies, diagnostic and therapeutic implications. Pharmacological unmasking of epigenetically silenced tumor suppressor genes in human cancers. Proteinases and proteinase inhibitors. The human tissue kallikrein gene family. Tumor biomarkers. Molecular mechanisms underlying anticancer drug resistance. Cellular senescence. Production and engineering of recombinant proteins.

Selected Publications Sotiropoulou G, Pampalakis G, Diamandis EP. (2009) Functional roles of human kallikrein-related peptidases. J Biol Chem 284: 32989-32994.

Pampalakis G, Diamandis EP, Katsaros D, Sotiropoulou G. (2009) Down-regulation of Dicer expression in ovarian cancer tissues. Clin. Biochem. 2009 Sep 24. [Epub ahead of print]

Pampalakis G, Prosnikli E, Agalioti T, Vlahou A, Zoumpourlis V, Sotiropoulou G. (2009) A tumor protective role for human kallikrein-related peptidase 6 in breast cancer mediated by inhibition of epithelial-to-mesenchymal transition. Cancer Res. 69: 3779-3787.

Sotiropoulou G, Pampalakis G, Lianidou ES, Mourelatos Z. (2009) Emerging roles of microRNAs as molecular switches in the integrated circuit of the cancer cell. RNA 15: 1443-1461.

Kioulafa M, Balkouranidou I, Sotiropoulou G, Kaklamanis L, Mavroudis D, Georgoulias V, Lianidou ES. (2009) Methylation of cystatin M promoter in breast cancer is associated with unfavorable prognosis. Int. J. Cancer, in press

Sotiropoulou G , Sommerhoff CP. (2008) Highlight on advances in proteolysis research. Biol. Chem. 389: 967-969.

Pampalakis G, Scorilas A, Sotiropoulou G. (2008) Novel splice variants of prostate- specific antigen and applications in diagnosis of prostate cancer. Clin. Biochem. 41: 591-597

Georgios A. Spyroulias Phone : +30 2610 997 721 Fax : +30 2610 997 714 e-mail : [email protected]

Associate Professor Ph.D. University of Crete

Current Focus of Research

Design/Simulation of bioactive molecules of pharmaceutical interest and Conformational Analysis of biomolecules through Nuclear Magnetic Resonance Spectroscopy (ΝΜR). Structural Bioinformatics applied in homology modeling of unknown structures of biomolecules, protein-protein/peptide/substrate interaction through molecular dynamics simulation, docking simulation protocols. In silico design and development of combinatorial libraries of compounds and virtual screening process towards the quest of lead-molecules with biological activity towards protein/enzyme targets, etc.

Selected Publications

Abriata LA, Banci L, Bertini I, Ciofi-Baffoni S, Gkazonis P, Spyroulias GA, Vila AJ, Wang S. Mechanism of Cu(A) assembly. Nat Chem Biol. 4, 599-601 (2008).

G. Dalkas, A.Papakyriakou, A. Vlamis-Gardikas, G.A.Spyroulias. Low molecular weight inhibitors of the protease Anthrax Lethal Factor. Mini Rev. Med. Chem. 8, 290-306 (2008).

Fousteris MA, Papakyriakou A, Koutsourea A, Manioudaki M, Lampropoulou E, Papadimitriou E, Spyroulias GA, Nikolaropoulos SS. Pyrrolo[2,3-a]carbazoles as Potential Cyclin Dependent Kinase 1 (CDK1) Inhibitors. Synthesis, Biological Evaluation, and Binding Mode through Docking Simulations. J Med Chem. 51, 1048- 1052 (2008)

A. Papakyriakou, G.A. Spyroulias, E. Sturrock, E. Manessi-Zoupa, P. Cordopatis. Simulated Interactions between Angiotensin-Converting Enzyme and Substrate Gonadotropin-Releasing Hormone: Novel Insights into Domain-Selectivity. Biochemistry 46, 8753-8765, (2007).

Z. Spyranti, G.A. Dalkas, G.A. Spyroulias, E.D. Matzourani, T. Mavromoustakos, I Friligou, T. Tselios, J. Matsoukas. Bioactive Conformations of Cyclic Myelin Basic Protein (MBP) Peptide Analogues: Exploring the Structural Requirements for Experimental Autoimmune Encephalomyelitis (EAE) Reversal. Journal of Medicinal Chemistry 50, 6039-6047 (2007).

L. Banci, I. Bertini, S. Ciofi-Baffoni, N.G. Kandias, G.A. Spyroulias, N.J. Robinson, M. Vanarotti, X.-C. Su. The delivery of copper for thylakoid import observed by NMR. Proc. Natl Acad Sci. USA 103, 8320-8325, (2006).

Stavros Topouzis

Assistant Professor Ph.D., Louis Pasteur University/Starsbourg I, France

Research Focus Discovery and characterization of molecular mechanisms contributing to vascular disease Interactions of polypeptide ligands with the cognate cell surface receptors that control growth and differentiation of vascular smooth muscle cells Molecular signalling mechanisms and differentiation processes affected by PDGF receptors in mesenchymal cells, with emphasis on vascular endothelial and smooth muscle cells

Selected publications

JA Thomas, RA Deaton, NiE Hastings, Y Shang, CW Moehlel, U Eriksson, S Topouzis, BR Wamhoff, BR Blackman, and GK Owens. Platelet-Derived Growth Factor-DD, a Novel Mediator of Smooth Muscle Cell Phenotypic Modulation, is Upregulated in Endothelial Cells Exposed to Atherosclerotic-Prone Flow Patterns.(In Press, Am J Physiol-Heart Circ Physiol).

SR Dillon, et al (2004) Interleukin 31, a cytokine produced by T cells, induces dermatitis in mice Nature Immunol., 5, 752-760

X Zhu, et al (2004) Myostatin signaling through Smad2, Smad3 and Smad4 is regulated by the inhibitory Smad7 by a negative regulatory feedback mechanism Cytokine, 26, 262-272

Hudkins KL, et al (2004) Exogenous PDGF-D is a potent mesangial cell mitogen and causes a severe mesangial proliferative glomerulopathy. J. Am. Soc. Nephrol., 15, 286-298

S Taneda, et al (2003) Obstructive uropathy in mice and humans: potential role for PDGF-D in the progression of tubulointerstitial injury. J. Am. Soc. Nephrol., 14, 2544-2555

W Xu, et al (2001) A soluble class II cytokine receptor, IL-22RA2, is a naturally occurring IL-22 antagonist. Proc. Natl. Acad. Sci. USA , 98, 9511-9116

S Topouzis and MW Majesky (1996) Smooth muscle lineage diversity in the chick embryo: Two types of aortic smooth muscle cell differ in growth and receptor- mediated transcriptional responses to transforming growth factor-β. Dev. Biol. , 178, 430-445

23 Antonios Tsarbopoulos e-mail: [email protected] and [email protected]

Associate Professor Ph.D. Michigan State University (East Lansing, Michigan)

Current Focus of Research Development of Analytical Methodologies, Based on Mass Spectrometric and Chromatographic Techniques, for the Analysis of Complex Biological Systems and the Structural Characterization of Natural Products and Biotechnology- derived Biomolecules. Detection of Non-Covalent Complexes by Electrospray Ionization (ESI) and Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry. Structure Analysis of Glycoproteins by ESI Mass Spectrometry. Probing Ternary Structures of Proteins by ESI and MALDI Mass Spectrometry. Isolation and Structure Determination and Bioavailability Studies of Plants‟ Active Substances; Development of New and Innovative Pharmaceuticals and Other Therapeutic Substances thereof. Detection, Identification and Monitoring Environmental Estrogens and Food Contaminants; Assessment of the Impact of Environmental Factors in Human Health.

Selected Publications F.N. Bazoti, J. Bergquist, K. Markides and A. Tsarbopoulos, “Localization of the Binding Site in the Non-Covalent Interaction between Amyloid-β Peptide (1-40) and Oleuropein Using Electrospray Ionization FTICR Mass Spectrometry”, J. Am. Soc. Mass Spectrom. 2008, 19, 1078-1085.

S.S. Petropoulou, E. Gikas, A. Tsarbopoulos and P. Siskos, “Gas Chromatographic- Tandem Mass Spectrometric Method for the Quantitation of Carbofuran, Carbaryl and their Main Metabolites in Applicators‟ Urine”, J. Chromatogr. A 2006, 1108, 99-110.

F.N. Bazoti, J. Bergquist, K. Markides and A. Tsarbopoulos, “Detection of the Non- Covalent Complex between Amyloid-β Peptide (1-40) and Oleuropein using Electrospray Ionization Mass Spectrometry”, J. Am. Soc. Mass Spectrom. 2006, 17, 568-575.

F.N. Bazoti, A. Tsarbopoulos, K. Markides and J. Bergquist, “Study of the Non- Covalent Interaction between Amyloid-ί Peptide and Melatonin using Electrospray Ionization Mass Spectrometry”, J. Mass Spectrom. 2005, 40, 182-192.

F.N. Bazoti, E. Gikas, C. Puel, V. Coxam and A. Tsarbopoulos, “Development of a Sensitive and Specific SPE-GC-MS/MS Method for the Determination of Elenolic Acid, Hydroxytyrosol and Tyrosol in Rat Urine”, J. Agr. Food Chem. 2005, 53, 6213- 6221.

E. Gikas, P. Kormali, D. Tsipi and A. Tsarbopoulos “Development of a Rapid and Sensitive SPE-LC-ESI MS/MS Method for the Determination of Chloramphenicol in Seafood”, J. Agr. Food Chem. 2004, 52, 1025-1030.

24 Socrates Tzartos Phone: +30 2610 969955 E-mail: [email protected], [email protected]

Professor Ph.D. Univ. of Athens, Greece

Current Focus of Research Nicotinic acetylcholine receptors in muscle and nerve (structure-function relationships, immunogenicity and pathogenicity). Study of myasthenia gravis as a model autoimmune disease. Towards the development of novel therapies.

Selected Publications

Sideris, S., Lagoumintzis, G., Kordas, G., Kostelidou, K., Sotiriadis, A, Poulas, K. and Tzartos, S.J. (2007) Isolation and functional characterization of anti-acetylcholine receptor subunit-specific autoantibodies from myasthenic patients: receptor loss in cell culture. J. Neuroimmunol. 189:111-117.

Kostelidou, K., Trakas,N. and Tzartos, S.J. (2007) Extracellular domains of the β, γ and ε subunits of the human acetylcholine receptor as immunoadsorbents for myasthenic autoantibodies: a combination of immunoadsorbents results in increased efficiency. J. Neuroimmmunol. 190:44-52

Zisimopoulou, P., Lagoumintzis, G., Poulas, K. and Tzartos, S.J. (2008). Antigen- specific apheresis of human anti-acetylcholine receptor autoantibodies from myasthenia gravis patients‟ sera using Escherichia coli-expressed receptor domains. J. Neuroimmmunol. 200: 133-141.

Zouridakis M, Zisimopoulou P, Eliopoulos E, Poulas K, and Tzartos, S.J (2009) Design and expression of human α7 nicotinic acetylcholine receptor extracellular domain mutants with enhanced solubility and ligand-binding properties. Bioch. Bioph. Acta. 1794: 355-66

Tsiamalos, P., Kordas, G., Kokla, A., Poulas, K., and Tzartos, S.J. (2009). Epidemiological and immunological profile of MuSK myasthenia gravis in Greece. Eur. J. Neurol. 16: 925-30

Instrumental facilities of the Department Spectrophotometers VIS-UV (Perkin-Elmer) Spectrometers IR (Perkin-Elmer) & FT-IR (Jasco) Complete HPLC system (Waters) Automatic Polarimeter (Schmidt & Haensch) Elemental Analyzer CHN (Carlo-Erba) Complete HPLC systems (Waters) Spectrophotometers VIS-UV (Shimatzu, Pharmacia) Probe Sonicator (Sonics and Materials) Fluorescence spectrophotometer (Simatsu) 6-Pot dissolution tester (Pharma-Test) Basic drug formulation pilot plan production lines (ERWEKA-KORSCH-HOMORX) Gamma Scintillation spectrometer (Packard) Viscometer (Brookfield) Small experimental animal facility Complete analytical and preparative HPLC systems (Pharmacia) Fast Protein Liquid Chromatography System (Pharmacia) Freeze Dryers (Labconco,4.5lit. and 6lit.) Water Pro Polishing Station (Labconco) Automatic Peptide Synthesizer (Advanced ChemTech, Model 90) Speed-Vac (Labconco) Centrifuges (Selecta, Ependorf) and Refrigerated Centrifuge (Hellenic Labware) Complete systems for standard liquid chromatography (LKB, Pharmacia) HF Apparatus (Multiple Peptide Synthesis Co.) γ-Counter (LKB) Microscope (Olympus) CO2 incubator (Lab-Line) Laminar Air-Flow Hood (Holten) Polarography instrumentation (EG&G) Eccentric Tableting machine (KORCH) Multivolume pycnometer (Micromeritics) PCR machine (Biometra) Orbital Shaker and incubator (Edmund Dooler) -80° C Freezer (FORMA 725) Storage container for liquid N2(FORMA CMR 8030) Transilluminator (GIBCO BRL, TFX 20M UV) Autoclave (Prestige SUPER EXTENDED 2100) Gel Drier (BioRad) Horizontal and vertical electrophoresis apparatuses (BioRad)

26 PROGRAMME OF STUDY

A. Undergraduate Studies The education system in Greece is based on semesters. There are two semesters per academic year. The first (Fall) semester begins in October 1st and ends in January 31st. Classes for the second (Spring) semester resume the 16th February and last until 10th of June. Students admitted for undergraduates studies need 10 semesters (5 years) in order to graduate, that is to obtain the diploma of Pharmacy (“Ptichion” in Greek). During each semester a student has to follow ca. 5 to 6 courses with a total of around 24-30 hrs attendance per week.

A course can consist of only lectures or lectures and practical work (laboratory). The courses offered in our Department are grouped in semesters (fall and spring semesters). Lectures can be followed by students at will, whereas attendance of laboratories is mandatory. In most courses there is not any formal assessment throughout the semester. In rare cases, lecturers offer partial exams within the semester and the grades obtained at these exams are taken into account in the final mark. However, in the labs the students are constantly examined usually orally on the theory and practice for each experiment they are to perform before or during their lab work and finally have to present written account of their results one week after the end of each exercise. All these are taken into account in the final mark together with the results of the final written examination that is associated with each particular lab. Failure to successfully perform up to 20% of the laboratory sessions results in the obligation for the student to make up the lost sessions by additional laboratory work. In cases in which students missed more than 20% of the laboratory work accompanying a course, they are not allowed to take the final exam of the course. The exam can only be taken after the successful completion of the laboratory work. The laboratory work usually includes four to six laboratory units per semester.

Courses are only offered in the Greek language and the faculty members teach the related material based on Greek textbooks. These textbooks usually are either the exact translation of the English counterparts or are based on them. Thus the content and the level of these Greek textbooks are similar to the corresponding english ones. For an ECTS student whose native language is not greek and his/her greek is not good enough to be able to study from a greek textbook there can always be easily found an english textbook with similar content to that his/her fellow greek students use. These textbooks are offered on loan by our university central library.

During their final year and in addition to the courses they follow the students have to carry out a short research project called Diploma Work under the supervision of a member of the academic staff. At the end of this work, students have to provide a written account of their results and often to present them orally. A grade is then assigned to the student by the supervisor involved reflecting the overall performance of the student. This grade should be at least 5 for a successfully completed Diploma Work. A senior year student is also required to make study visits to both Pharmaceutical Industries and Drug Stores. The duration of the practice period is approximately two semesters. Credit for this activity is given through the courses named Pharmaceutical Practice I & II. The grade assigned for these courses is the average of student performance, student reports and final examination grade.

A course is considered as being successfully passed only when the student has acquired at least the grade 5 out of 10 in the associated exams. The grading scheme, based on a 0 to 10 scale is as follows: EXCELLENT 8.5 to 10 VERY GOOD 6.5 to 8.5 (not included) GOOD 5.0 to 6.5 UNSATISFACTORY 0 to 5.0 (not included) The minimum passing grade is 5.0 and all the grades are expressed as integers. However a course associated with lab work requires in addition also successful completion of the lab work and for the final mark both the grade in the exam and the lab performance are considered with factors which vary from lab to lab. Exams are offered to the students after the end of each semester and repeat exams in September. However students who have failed in these exams or not participated at all can sit for these exams as many times as they wish in the following exams periods. A student is considered as having completed his/her studies in our Department only when he/she has passed all the exams associated with all courses consisting our curriculum.

Taking into account the Greek higher education system the basic requirements of the ECTS system for 30 credits for each semester was met in the Department by initially assigning for each 1 hr per week per semester of lecture and lab work 1 ECTS credit. Additional ECTS credits were then added to those of the course that are considered as the most hard for the students to be passed that is those requiring higher student workload.

ECTS students who have studied for at least a year in our institution can be considered as candidates to obtain the Diploma (Ptychion) in Pharmacy offered by the Department for undergraduate studies. The ECTS Committee of our Department dealing with the recognition of studies carried out abroad will consider students transcripts of records and their performance at our Department. Courses successfully completed abroad will then correlated to those in Patras. If there is no need for additional courses to be taken by the student in Patras in order to fulfill the requirements imposed by our curriculum this committee will propose the General Assembly of the Department to offer our Diploma (Ptichion) to that particular student. Otherwise, the student will have to attend and successfully pass all those courses that are needed to complete our curriculum.

B. Graduate Studies Graduate studies in the Department of Pharmacy lead to: A. Graduate Diploma of Specialization (GDS) B. Doctorate degree (Ph.D.) 1. GDS Five specializations are offered: A) Industrial Pharmaceutics and Drug Analysis, B) Pharmaceutical Chemistry-Natural Products: Design, Synthesis and Analysis of Bioactive Compounds; C) Molecular Pharmacology – Clinical Pharmacy; D) Pharmaceutical Biotechnology and Biomedicine; E) Pharmaceutical Marketing. The GDS involves two years of studies and each graduate student has to take a total of six or eight courses depending on the specialization. Instruction is in Greek or English depending on the class composition. The candidate has to pass the exams associated

28 with these courses. Exams take place at the end of each semester. The minimum passing grade is 5 out of 10. Repeat exams for both semesters take place in September. During the two years the student carries out a short, novel, research project and presents the results both written and orally. A student who has successfully completed the requirements for GDS can continue his/her studies by pursuing a Ph.D degree.

2. Doctorate Degree For the doctorate degree a supervisory committee of three members is assigned upon the approval of the Department and the student conducts research under the direction of the chairman of the supervisory committee. The average time required for a Ph.D degree is four years. During the first two years a graduate student has to sucessfully take 4 core courses and 4 electives regardless of the specialization under which they are offered.

29 List of Undergraduate Courses PDU - 111 General and Inorganic Chemistry PDU - 112 Physics PDU - 113 Informatics PDU - 114 Apllied Mathematics PDU - 115 General Botanics PDU - 116 Introduction to Pharmaceutical Science PDU - 117 Foreign Language I PDU - 121 Analytical Chemistry PDU - 122 Organic Chemistry PDU - 123 Biochemistry I PDU - 124 Cell Biology PDU - 125 Morphology of Human Body PDU - 126 Foreign Language II PDU - 211 Synthetic Organic Chemistry PDU - 212 Biochemistry II PDU - 213 Physiology I PDU - 214 Physical Chemistry PDU - 215 Laboratory of General and Analytical Chemistry PDU - 216 Foreign Language III PDU - 221 Pharmaceutical Chemistry I PDU - 222 Pharmaceutical Microbiology PDU - 223 Physical Pharmacy PDU - 224 Molecular Biology - Genetics PDU - 225 Physiology II PDU - 226 Foreign Language IV PDU - 311 Pharmaceutical Chemistry II PDU - 312 Pharmacology I PDU - 313 Chemistry of Natural Products PDU - 314 Instrumental Analysis I - Separation Techniques - Electrochemical Analysis PDU - 315 Pharmaceutical Immunology PDU - 321 Pharmacognosy I PDU - 322 Pharmaceutical Chemistry III Drugs against Infectious Diseases-Inorganic Drugs PDU - 323 Instrumental Analysis II - Organic Spectroscopy and Spectrometry PDU - 324 Pharmaceutical Technology I PDU - 325 Pharmacology II PDU - 412 Pharmacognosy II PDU - 413 Pharmaceutical Chemistry IV PDU - 414 Pharmaceutical Biotechnology PDU - 415 Clinical Pharmacy PDU – 416 Pharmaceutical Technology II PDU - 421 Toxicology PDU - 424 Biopharmaceutics and Pharmacokinetics PDU - 426 Molecular Pharmacology PDU - 428 Bioinorganic Chemistry and Molecular Simulations PDU – 429 Pathology – First Aids PDU - 511 Bioethics and Law PDU - 512 Pharmaceutical Practice I PDU - 513 Diploma Work I (Laboratory) PDU – 514 Diploma Work I (Bibliography) PDU – 515 Chemistry and technology of Cosmetics

30 PDU - 516 Research Methodology I PDU - 521 Basics on Physics of Nuclear Pharmacy and Radiopharmacy PDU - 522 Pharmaceutical Practice II PDU - 523 Diploma Work II (Laboratory) PDU – 524 Diploma Work II (Bibliography) PDU - 525 Pharmacoeconomy PDU - 526 Research Methodology II

31 PROGRAMME PLAN

Undergraduate studies The three numerals following each course code number indicate lecture hours, laboratory hours and ECTS credits respectively.

COURSE SUMMARY TABLE

FIRST YEAR SEMESTER I SEMESTER II PDU-111 4 4 5 PDU-121 4 0 5 PDU-112 3 0 3 PDU-122 4 0 6 PDU-113 2 4 5 PDU-123 4 3 8 PDU-114 3 0 4 PDU-124 4 0 5 PDU-115 4 3 6 PDU-125 3 0 4 PDU- 116 2 3 5 PDU-126 3 0 2 PDU -117 3 0 2

SECOND YEAR SEMESTER III SEMESTER IV PDU-211 4 4 6 PDU-221 4 4 7 PDU-212 4 4 6 PDU-222 2 3 4 PDU-213 5 2 6 PDU-223 4 3 7 PDU-214 4 3 6 PDU-224 3 3 5 PDU-215 1 4 4 PDU-225 4 0 5 PDU-216 3 0 2 PDU-226 3 0 2

THIRD YEAR SEMESTER V SEMESTER VI PDU-311 4 0 7 PDU-321 4 4 6 PDU-312 4 2 6 PDU-322 4 0 6 PDU-313 4 0 5 PDU-323 4 3 6 PDU-314 4 3 8 PDU-324 5 3 7 PDU-315 3 0 4 PDU-325 4 0 5

FOURTH YEAR SEMESTER VII SEMESTER VIII PDU-412 4 4 6 PDU-421 3 0 4 PDU-413 4 4 6 PDU-424 4 4 8 PDU-414 3 3 6 PDU-426 4 4 8 PDU-415 3 0 4 PDU-428 3 3 6 PDU-416 5 4 8 PDU-429 2 2 4

32 FIFTH YEAR SEMESTER IX* SEMESTER X* PDU-511 2 - 6 PDU-521 5 0 6 PDU-512 - 10 10 PDU-522 - 10 10 PDU-513 - - 9 PDU-523 - - 9 PDU-514 - - 9 PDU-524 - 0 9 PDU-515 2 - 5 PDU-525 2 0 5 PDU-516 - - 5 PDU-526 - - 5

*Each student has to select either PDU-513, 516, 523, 526 or PDU-514, -515, -524, - 525

DESCRIPTION OF UNDERGRADUATE COURSE UNITS

The following courses are offered by the Department of Pharmaceutical arranged as appear in the program plan. The prefix for all undergraduate courses is PDU (Pharmaceutical Department Undergraduate). The first numeral of the course unit number indicates the Academic year and the second numeral indicates the period (1 for Fall and 2 for Spring).

ECTS Cource code : PDU-111 Title : General and Inorganic Chemistry Lecturer : V. Magafe Type : Lectures (4h/w) Period : Fall ECTS credits : 6

Content : Atomic Structure. Electronic Configurations of Atoms and Periodic Table. Ionic and Covalent Bond. Gases, Liquids and Solids. Intermolecular Forces and Phase Diagrams. Solutions. Chemical Kinetics. Chemical Equilibrium. Acids and Bases. Chemical Thermodynamics. Redox Systems and Electrochemistry.

ECTS Course code : PDU-112 Title : Physics Lecturer : G. Kagadis; E. Kostaridou; A. Bezerianos Type : Lectures (3h/w) Period : Fall ECTS credits : 3

Contents : Introduction to Mechanics. Statics of fluids. Bones. Electricity and magnetism. Static Electricity. DC and AC Currents. Electrical properties of nerves and induced potentials. Production, transport and measurement of biodynamic currents. Effect of electricity in human body. Biomagnetics : Comparative Introduction, Magnetism of nerves and induced magnetic fields.

ECTS Course code : PDU-113 Title : Informatics Lecturer : P. Klepetsanis and G. Spyroulias Type : Lectures (3h/w) ; Laboratory (4h/w) Period : Fall ECTS credits : 5

34 ECTS Course code : PDU-114 Title : Applied Mathematics Lecturer : V. Piperigou; E. Petropoulou; F. Zafeiropoulou Type : Lectures (3h/w) Period : Fall ECTS credits : 4

Contents : One variable functions, derivative and rules of differentiation, study of real functions (limits, inflection points, continuity, asymptotics and graphical presentation), differential calculus, indefinite (derivative and calculation methods) and definite integrals.

CTS Cource code : PDU-115 Title : General Botanics Lecturer : D. Tzanoudakis, D. Christodoulakis Type : Lectures (4h/w) and laboratory (3h/w) Period : Fall ECTS credits : 6

Contents : Introduction to Botanics. Groups of plants, chemical composition of plants. Characteristics of life, plants and animals. Vegetable cells and tissues: description and properties.

ECTS Cource code : PDU-116 Title : Introduction to Pharmaceutical Sciences Lecturer : K. Avgoustakis; G. Pairas; E. Papadimitriou Type : Lectures (2h/w) and laboratory (3h/w) Period : Fall ECTS credits : 5

Contents : History of pharmacy. Studies at the Department of Pharmacy, University of Patras. Professional possibilities for pharmacists. Development of new drugs – Pharmacopoeias.

Laboratory course: Search of literature. Handling of laboratory equipment and general laboratory procedures. Sterilization – Laminar flow hoods. Microscopy. Culture of prokaryotic cells in liquid and solid media. Isolation and culture of eucaryotic cells.

35 ECTS Course code : PDU-117 Title : Foreign Language I Lecturer :Staff Type : Lectures (3h/w) Period : Fall ECTS credits : 2

Contents : Basics of a foreign language (English, German, Italian, French, Russian).

ECTS Course code : PDU-121 Title : Analytical Chemistry Lecturer : F. Lamari and G. Pairas Type : Lectures (4h/w) Period : Spring ECTS credits : 6 Contents : Qualitative Inorganic analysis : I,II,III and IV cation groups, anions, buffers and complex ions. Quantitative Inorganic analysis : sampling, sample treatment accuracy and precision, gravimetric analysis, titrimetric analysis, analytical and volumetric instruments, techniques and reagents.

ECTS Cource code : PDU-122 Title : Organic Chemistry Lecturer : P. Cordopatis, P. Magriotis and S. Nikolaropoulos Type : Lectures (4h/w) Period : Spring ECTS credits : 6

Contents : Introduction. Structure and reactivity: Hybridisation, bonding in carbon compounds, Inductive and field effects. Mesomeric effects, hyperconjugation, steric effects. Reagent types and Reaction types. Energetics and kinetics of reaction. The strengths of acids and bases. SN1, SN2, SNi. Carbocations. Electron-deficient N and O atoms and their reactions. Alcohols. Aldehydes. Nitriles. Ketones. Carboxylic acids. Amides. Amines. Nucleophilic addition to C=O. Elimination reactions.

ECTS Cource code : PDU -123 Title : Biochemistry I Lecturers : F. Lamari; G. Spyroulias; S. Tzartos and K. Poulas Type : Lectures (4h/w) and laboratory (3h/w) Period : Spring ECTS credits : 6

Contents :

36 Amino acids and proteins. Bioenergetics and high energy compounds. Enzymes and kinetics of enzymic reactions. Rules of kinetics reactions. Carbohydrates metabolism (part 1). Krebbs and glyoxalic cycles. Biological oxidations. Photosynthesis, photosystems I and II and Calvin cycle, Carbohydrates metabolism (part 2). Biochemistry of connecting tissues. Nucleic acids. Biological membranes

ECTS Course code : PDU-124 Title : Cell Biology Lecturer : G. Patrinos and G. Sotiropoulou Type : Lectures (4h/w) Period : Spring ECTS credits : 6

Contents : Cellular structure and function (origin of life, viruses, prokaryotic cells, eucaryotic cells. Macromolecules of the cells (proteins, nucleic acids, carbohydrates, lipids). How cells are studied (microscopy, cell culture, purification of macromolecules). Plasma membrane (lipid bilayer, membrane proteins and carbohydrates, membrane transport of micro and macro molecules). Cell to cell interactions (cell junctions, cell-cell recognition and adhesion). Genetic code and proteins (DNA replication, DNA repair, transcription, translation, ribosomes and protein synthesis). Endoplasmic reticulum, Golgi apparatus, ribosomes, post translational modifications of proteins, secretion). Degradation of macromolecules (lysosomes, peroxisomes). Cytoskeleton and cell movements (organization of the cytoskeleton, microtubules, microfilaments, intermediate filaments, ciliary movement). Mitochondria and chloroplasts (structure, functions, evolution). Organization of nucleic acids (DNA organization in viruses, prokaryotic and eucaryotic cells). Cell growth and division (cell cycle, cell division, mechanics of cell division, cell death).

ECTS Cource code : PDU-125 Title : Morphology of Human body Lecturer : M. Assimakopoulou; K. Gyftopoulou; E. Petrou ; G. Sotiropoulou-Bonikou and I. Varakis Type : Lectures (3h/w) Period : Spring ECTS credits : 4

Contents : Human anatomy, description of cells and tissues and general Embryology.

37 ECTS Course code : PDU-126 Title : Foreign Language II Lecturer :Staff Type : Lectures (3h/w) Period : Spring ECTS credits : 2

Contents : Basics of a foreign language (English, German, Italian, French, Russian).

ECTS Cource code : PDU-211 Title : Synthetic Organic Chemistry Lecturer : E. Fousteris ; S. Nikolaropoulos and G. Pairas Type : Lectures (4h/w) and Laboratory (4h/w) Period : Fall ECTS credits : 6

Contents : Preparation and transformation of organic functional groups. Formation of C-C bond. Methods for Cyclic compounds synthesis.

ECTS Cource code : PDU- 212 Title : Biochemistry II Lecturer : F. Lamari; G. Spyroulias; S. Tzartos and K. Poulas. Type : Lectures (4h/w) and laboratory (4h/w) Period : Fall ECTS credits : 6

Contents : Porphyrines, hormones and vitamins. Proteins turnover, signal transduction, RNA translation control, metabolism of nucleoticides. Nucleic acids and genetic code. Biosynthesis of proteins, biochemistry of viruses. Biochemistry of the immune response. Gene rearrangements and recombinant DNA. Integration of metabolism. Cellular homeostasis and control of genetic programming.

ECTS Cource code : PDU-213 Title : Physiology I Lecturer : F. Aggelatou, G. Kostopoulos, G. Voukelatou, A. Mitsakos Type : Lectures (5h/w) and laboratory (2h/w) Period : Fall ECTS credits : 6

Contents : General physiology. Physiology of blood. Physiology of circulating and breathing systems.

ECTS Cource code : PDU-214 Title : Physical Chemistry Lecturer : C. Kontoyannis, P. Klepetsanis, and M. Orkoula Type : Lectures (4h/w) and laboratory (3h/w) Period : Fall ECTS credits : 6

Contents : Ideal and non-ideal gases. Kinetic theory and van der Waals equation. Thermodynamics. Chemical equilibrium. Phase diagrams. Properties of mixtures and solutions. Chemical Kinetics, determination of order and constant of reaction, homogeneous and heterogeneous catalysis and reaction mechanisms. Electrochemistry, activity and activity coefficients, electrical double layer, electrochemical equilibria and electrochemical kinetics.

ECTS Cource code : PDU-215 Title : Laboratory of Analytical Chemistry Lecturer : G. Pairas, G. Spyroulias and T. Zafeiropoulos. Type : Lectures (1h/w) and laboratory (4h/w) Period : Fall ECTS credits : 4

Contents : Quanlitative Inorganic analysis: I,II,III and IV cation groups, anions, buffers and complex ions. Quantitative Inorganic analysis: sampling, sample treatment accuracy and precision, gravimetric analysis, titrimetric analysis, analytical and volumetric instruments, techniques and reagents.

ECTS Course code : PDU-216 Title : Foreign Language III Lecturer :Staff Type : Lectures (3h/w) Period : Fall ECTS credits : 2

Contents : Basics of a foreign language (English, German, Italian, French, Russian).

ECTS Cource code : PDU-221 Title : Pharmaceutical Chemistry I Lecturer : E. Fousteris and S. Nikolaropoulos Type : Lectures (4h/w) and laboratory (4h/w)

39 Period : Spring ECTS credits : 7

Contents : Introduction. Mechanisms of Drug Action. Local Anesthetics General Anesthetics. Laxatives. Antiemetics. Antacids. Drugs for Peptic Ulceration. Antidiarrhoeal drugs. Drugs for the treatment of chronic liver diseases. Diuretics. Antiarrythmics. .beta.-blockers. Antihypertensives. Sympathomimetics. Anti-anginal drugs. Anticoagulants. Thrombolytics. Haemostatics. Anticancer drugs: Actions of drugs, Pre-treatment evaluation, Combination Chemotherapy, Regiments of Cancer Chemotherapy, Adverse effects.

ECTS Course code : PDU – 222 Title : Pharmaceutical Microbiology Lecturer : S. Tzartos and K. Poulas Type : Lectures (2h/w) and laboratory (3h/w) Period : Spring ECTS credits : 4

Contents : Introduction to microbiology. Bacteria: nomenclature, classification, growth and metabolism. Microbiological techniques. Nucleic acids of microorganisms, viruses, development and augmentation of microrganisms, influence of environment on microorganisms. Immunological techniques. Clinical bacteriology, clinical virusiology, clinical micitology and clinical paracitology.

ECTS Course code : PDU – 223 Title : Physical Pharmacy Lecturer : P. Klepetsanis Type : Lectures (4h/w) and laboratory (3h/w) Period : Spring ECTS credits : 6

Contents: Solubility and Distribution of Drugs. Complexation and Protein Binding. Interfacial Phenomena. Colloids. Rheology. Coarse Dispersions and Emulsions. Dissolution of pharmaceutical forms - theory.

ECTS Cource code : PDU-224 Title : Molecular Biology - Genetics Lecturer : G. Patrinos Type : Lectures (3h/w) and laboratory (3h/w) Period : Spring ECTS credits : 5

40 ECTS Cource code : PDU-225 Title : Physiology II Lecturer : F. Aggelatou; A. Mitsakou and G. Voukelatou Type : Lectures (4h/w) Period : Spring ECTS credits : 6 Contents : Physiology of the urinary system. Adjustment of volume, composition and pH of human liquids. Physiology of digestive system. Physiology of genetic system. Physiology of autonomous nervous system. Physiology of the hypothalamus.

ECTS Course code : PDU-226 Title : Foreign Language IV Lecturer :Staff Type : Lectures (3h/w) Period : Fall ECTS credits : 2

Contents : Basics of a foreign language (English, German, Italian, French, Russian).

ECTS Course code : PDU-311 Title : Pharmaceutical Chemistry II Lecturer : C. Camoutsis Type : Lectures (4h/w) Period : Fall ECTS credits : 7

Contents : Synthesis, uses, doses and pharmacological activity of organic and heterocyclic drugs. Analgesics, Antipyretics, Antihistaminics, Acridines, Anticoagylatns (Cumarins and chromones), Barbiturates, Benzodiazepines, Benzo-1,2,4-thiadiazine dioxides, Benzofurans, Benzimidazoles, Dibenzocycloheptadienes, Dibenzothiazepines, Dibenzoxazepines, Dibenzapines, Dihydrobenzo-1,2,4-thiadiazine dioxides, indoles, Phenothiazines, Quinolines, Synthetic Sweetening Agents (Saccharin), Sympathomimetic Amines, Sulfonamides, Thioxanthenes, Thyroid Hormones.

ECTS Course code : PDU-312 Title : Pharmacology I Lecturer : E. Papadimitriou; A. Papapetropoulos and S. Topouzis Type : Lectures (4h/w) and laboratory (2h/w) Period : Fall ECTS credits : 6

41 Contents: Introduction to pharmacology. General principles of drug action (introduction to pharmacodynamics, receptors, agonist-antagonists). Dose-response curves. Essential pharmacokinetics: absorption, distribution, metabolism and excretion of drugs. Guidelines for the selection of appropriate dosing regements. Basic and clinical evaluation of new drugs. Drugs of the autonomic nervous system. Local and General anesthetics. Drugs that affect the central nervous system: sedatives, hypnotics, anti- epileptic medication, anxiolytics, anti-depressants, drugs used to treat psychosis, mood-affecting medications, opioids, drugs used to treat Parkinson‟s disease.

ECTS Course code : PDU-313 Title : Chemistry of Natural Products Lecturer : P. Cordopatis Type : Lectures (4h/w) Period : Fall ECTS credits : 5 Recommended reading : J. Mann, R.S. Davidson, J.B. Hobbs, D.V. Banthape and J.B. Harborne "Natural Products" Longman, UK 1994.

Contents: Introduction. Carbohydrates (The chemistry of mono-, oligo- and polysaccharides). Amino acids and Peptides (The chemical synthesis of peptides). Alkaloids. Nucleosides, Nucleotides and Nucleic Acids (Oligonucleotide synthesis). Terpenes. Classics in Total Synthesis. Combinatorial Chemistry.

ECTS Course code : PDU-314 Title : Separation Techniques and Electrochemical Analysis Lecturer : C. Kontoyannis; A. Tsarbopoulos and M. Orkoula Type : Lectures (4h/w) and laboratory (3h/w) Period : Fall ECTS credits : 7 Recommended reading: D. Skoog, F.J. Holler and T. Nieman, Principles of Instrumental Analysis, 5th Edition, Saunders College Publishing

Contents : Chromatographic Techniques: Introduction to chromatographic techniques, gas and liquid chromatography, capillary electrophoresis. Methods of detection and applications in pharmaceutical analysis. Introduction to electroanalytical techniques, Potentiometry (reference electrodes, ion selective and enzyme electrodes, direct potensiometric measurements, potensiometric titrations), Coulometry, Voltammetry (Instrumentation, Polarography, Cyclic Voltametry), Amperometric techniques.

42 Laboratory practice on the analysis of active pharmaceutical ingredients employing electrochemical and chromatographic techniques.

ECTS Course code : PDU- 315 Title : Pharmaceutical Immunology Lecturer : K. Poulas; S. Tzartos and G. Sivolapenko. Type : Lectures (3h/w) and laboratory (2h/w) Period : Fall ECTS credits : 5

Contents : Non-specific immune response. Antigen recognition and presentation. Cellular immunity. Effector mechanisms of cellular immunity. Humoral immunity. Effector mechanisms of humoral immunity. Tolerance and autoimmunity. Immune response in cancer and transplantations. Hypersensitivity. Immunodeficiencies

ECTS Cource code : PDU-321 Title : Pharmacognosy I Lecturer : P. Cordopatis; F. Lamari and V.Magafa Type : Lectures (4h/w) and laboratory (4h/w) Period : Spring ECTS credits : 6

Contents : Introduction: definition of pharmacognosy, crude drugs, evaluation and classification of drugs. Medicinal plants: plant cell and tissue culture-production and isolation of secondary metabolites. Photosynthesis. Classification of natural products. Basic biosynthetic pathways for the production of natural products. Carbohydrates and related crude drugs. Natural products derived biosynthetically from the shikimic acid and the medicinal plants containing them: tannins, phenylpropanoids, flavonoids, essential oils, coumarins, balsams. Natural products derived biosynthetically from acetate and the medicinal plants containing them: lipids and waxes, anthraquinones, monoterpenoids, sesquiterpenoids, diterpenoids, triterpenoids, tetraterpenoids and carotenoids, cardiac glycosides, saponins. Laboratory courses: Principles of thin-layer chromatography -TLC analysis of aminoacids, analgesic drugs and carotenoid pigments in spinach-. Essential oils -Isolation of eugenol from cloves, isolation of cinamonaldehyde from cinammon, synthesis of cinamonaldehyde semicarbazone. Lipids: Isolation of trimyristin and myristicin from nutmeg. Synthesis of azelaic acid. Carbohydrates: -Isolation of casein and lactose from milk, identification of monosaccharides, isolation of pectin from lemon peels. Flavonoids - Isolation of hesperidin from orange peel and naringin from grapefruit peel-. Carotenoids -Isolation of chlorophyl and carotenoid pigments from spinach, capsanthin from paprika and lycopene from tomatoes.

ECTS Cource code : PDU-322 Title : Pharmaceutical Chemistry III Lecturer : P. Magriotis Type : Lectures (4h/w) and laboratory (3h/w) Period : Spring ECTS credits : 6

Contents : Synthesis, uses, doses and pharmacological activity of Steroids. (Estrogens, Non-Steroidal Estrogens, Progesterone Derivatives, Androgens, 19-Noradrogens, Cortisone Derivatives).

ECTS Course code : PDU-323 Title : Organic Spectroscopy and Mass Spectrometry Lecturer : C. Kontoyannis; M. Orkoula ; G. Spyroulias and A. Tsarbopoulos Type : Lectures (4h/w) and laboratory (3h/w) Period : Spring ECTS credits : 6 Recommended reading: D. Skoog, F.J. Holler and T. Nieman, Principles of Instrumental Analysis, 5th Edition, Saunders College Publishing

Contents : Atomic spectroscopy: Introduction to spectroscopic methods, components of optical instruments, introduction to optical atomic spectroscopy, atomic absorption spectroscopy, atomic emission spectroscopy, atomic mass spectrometry. Molecular spectroscopy: Introduction to ultraviolet /visible molecular absorption spectroscopy, Application of UV/Vis molecular absorption spectroscopy, molecular luminescence spectroscopy, infrared spectroscopy, Raman spectroscopy, Nuclear magnetic resonance spectroscopy (spectra assignment and structure determination). Mass spectrometry: Introduction, methods of ionization and applications in pharmaceutical analysis.

Laboratory practice on the analysis of active pharmaceutical ingredients employing spectroscopic techniques.

ECTS Cource code : PDU-324 Title : Pharmaceutical Technology I Lecturer : S. Antimisiaris and K. Avgoustakis Type : Lectures (5h/w) and laboratory (3h/w) Period : Spring ECTS credits : 6

44 Contents : Pharmacy Practice. The Prescription. Introduction to Pharmaceutical Technology. Principles of dosage form design. Preformulation. Industrial Pharmacy: Manufacture of Drug Products, GMP, Pharmaceutical processing. Powder Technology. Sterilization Principles and Practice. Aseptic Techniques. Design and Operation of Clean Rooms.

ECTS Course code : PDU-325 Title : Pharmacology II Lecturer : E. Papadimitriou; A. Papapetropoulos and S. Topouzis Type : Lectures (4h/w) Period : Spring ECTS credits : 6

Contents: Mechanisms of action, pharmacokinetics, adverse effects and drug-drug interactions for drugs used to treat all major diseases. Drugs for the treatment of cardiovascular diseases (drugs for the treatment of congestive heart failure and angina, antiarrythmics, anti-hypertensives, diuretics, antigoagulants, thrombolytics, inhibitors of platelets, drugs used for the treatment of hyperlipidemia), drugs used in the treatment pulmonary (asthma, COPD) and GI tract disorders (ulcers, nausea, and motility disorders), chemotherapeutic agents (anti-tumor, beta-lactam antibiotics & other inhibitors of cell wall synthesis, chloramphenicol, tetracyclines, macrolides, aminoglycosides, sulfonamides, quinolones disinfectants, antiseptics, antiprotozal, anti-mycobacterial, anti-fungal, anti-viral, anti-parasitic drugs), non-steroidal antiinflammatory drugs, corticosteroids, medications used for endocrine disorders (diabetes, replacement therapy for the thyroid gland and other endocrine organs), contraceptives, immunopharmacology, vitamins.

ECTS Cource code : PDU-412 Title : Pharmacognosy II Lecturer : P. Cordopatis; F. Lamari and V.Magafa Type : Lectures (3h/w) Period : Fall ECTS credits : 3

ECTS Course code : PDU-413 Title : Pharmaceutical Chemistry IV Lecturer : G. Pairas ; C. Camoutsis ; M. Fousteris ; S. Nikolaropoulos ; P. Magriotis Type : Lectures (4h/w) and laboratory (4h/w) Period : Fall ECTS credits : 6

ECTS Course code : PDU-414 Title : Pharmaceutical Biotechnology Lecturer : G. Sotiropoulou Type : Lectures (3h/w) and Laboratory (3h/w) Period : Fall ECTS Credits : 6

Contents : Introduction to molecular biotechnology. Historical breakthroughs in molecular genetics and the development of recombinant DNA technology. DNA is the primary genetic material. The Hershey-Chase experiment. Structure and biochemistry of nucleic acids. Structure of prokaryotic and eukaryotic genes. The complexity of the human genome. The genetic elements that control gene expression in prokaryotes and eukaryotes. Methods of creating recombinant DNA molecules. The Boyer-Cohen experiment. Restriction endonucleases and other enzymes for DNA manipulation. Cloning vectors. Basic molecular biology techniques (isolation, radiolabeling and hybridization of nucleic acids, Northern, Southern and dot blotting, autoradiography and in vitro transcription). Chemical synthesis of DNA. DNA sequencing. Molecular diagnostics (nucleic acid probes). The Polymerase Chain Reaction, PCR: methodology and applications (quantitation of RNA and DNA targets, diagnosis and characterization of human genetic diseases, development and quality assurance testing of biopharmaceuticals). RT-PCR. Quantitation of gene expression. Differential gene expression. cDNA and genomic libraries. Gene cloning. Gene cloning in E. coli and yeast. Antisense technology and antisense drugs. In vitro mutagenesis. Gene transfer into mammalian cells. Reporter genes-promoter analysis. Heterologous production of recombinant proteins in eukaryotic cells. Protein pharmaceuticals and vaccines produced by recombinant DNA technology. Protein engineering. Monoclonal antibodies: hybridoma technology, purification and applications. Immunoassays. Engineering of antibodies. Chimeric, humanized and catalytic antibodies. Immunotoxins/Immunoconjugates. Phage display. Microbial synthesis of low-MW biological molecules, antibiotics, biopolymers. Immobilization of enzymes and cells. Biocatalysts and applications. Introducing foreign genes into mice. Animal models for human diseases. Transgenic animals-production of pharmaceuticals. Genetic engineering of plants. Human somatic cell gene therapy. Stem cell research. Cloning of higher mammals. The human genome project. Genomics. Functional Genomics. Proteomics. Pharmacogenomics-Pharmacoproteomics. SNPs. Personalized prescriptions. Molecular target-based drug development. Biochips (DNA microarrays)-implications for the molecular diagnosis and the evaluation of drug targets and drug toxicity. Bioinformatics. Clinical trials. Intellectual property. Ethical and social implications of biotechnology.

Laboratory Course: 1. Manipulating DNA (Video, E. J. Wood) 2. Cell fractionation and isolation of sub-cellular organelles. Microscopy. 3. Isolation and analysis of human genomic DNA. 4. Genetic engineering I: bacterial transformation, digestion of plasmid DNA with restriction enzymes, agarose electrophoresis and analysis of restriction fragments. 5. Genetic engineering II: isolation, purification and quantitation of plasmid DNA. 6. Polymerase Chain Reaction, PCR: application for site-directed mutagenesis of recombinant proteins. 7. Polymerase Chain Reaction, PCR: application for the detection of polymorphisms in human DNA. 8. Bioinformatics I: biotechnology databases, NCBI, PubMed, BLAST search, PharmInfoNet/Pharmlinks. Protein structure database, PDB (crystallographic structures of protein drug targets). The RasMol program of molecular modeling. 9. Bioinformatics II: a) physical maps, OMIM. b) retrieval of nucleic acid and protein sequences from Genbank/EMBL, analysis using PC Gene, GenTools/PepTools software (restriction maps, translation, hydrophylicity/hydrophobicity/antigenicity plots, protein alignments, phylogenetic trees, protein families identification of functional motifs in proteins). 10. Hybridoma technology and monoclonal antibodies (a video guide, CSHL Press). Applications of PCR (A Decade of PCR, CSHL Press).

ECTS Course code : PDU-415 Title : Clinical Pharmacy Lecturer : G. Sivolapenko Assoc. Professor Type : Lectures (3h/w) Period : Fall ECTS Credits : 3

Contents : Introduction to Clinical Pharmacy: Scope, importance, emphasis on application of clinical pharmacotherapeutics, mechanisms of drug action and human pathophysiology. Drug interactions, Drug allergies, GI problems, Special age groups, Drug abuse, Analgesia, Hepatic problems, Endocrine diseases (diabetes, thyroid and growth hormone deficiencies), Contraceptives, Anemias and clotting disorders, AIDS, Depression, Dementias, Cardiovascular diseases, Eye infections, Applications of biotechnology in clinical pharmacy, The use of pharmacogenomics in clinical pharmacy.

ECTS Cource code : PDU-416 Title : Pharmaceutical Technology II Lecturer : G.S.Antimisiaris and K. Avgoustakis Type : Lectures (5h/w) and Laboratory (4h/w) Period : Fall ECTS credits : 8

47 Recommended reading: H.C. Ansel, Introduction to Pharmaceutical Dosage Forms; Remmighton Pharmacopeia

Contents : Pharmaceutical Dosage Forms: Pharmaceutic Ingredients, Product formulation, and Good Manufactiring Practice. Peroral Solids, Capsules, Tablets and Controlled- release dosage forms. Oral Solutions, Syrops and Elixirs. Oral Suspensions, Emulsions, Magmas and Gels. Injections, Biological Products and Sterile Fluids, Aerosols, Inhalations and Sprays. Transdermal delivery systems, Ointments, Creams, Lotions and Other Preparations. Ophthalmic Preparations. Ear, Nose and Topical Preparations. Suppositories and other rectal, vaginal and urethral Preparations.

ECTS Course code : PDU-421 Title : Toxicology Lecturer : E. Giannopoulou and S. Topouzis Type : Lectures (3h/w) Period : Spring ECTS credits : 4

Contents : Introduction to toxicology. Pharmaceuticals and toxicological means. Poisons, mean lethal dose, selectively toxicity, entrance ways, distribution, and metabolism of poisons in human body. Influence parameters for compounds toxicity. Therapy from toxical compounds. Air pollution from inorganic and organic pollutants. Poisoining from pharmaceuticals.

ECTS Cource code : PDU-424 Title : Biopharmaceutics and Pharmacokinetics Lecturer : G. Sivolapenko Type : Lectures (4h/w) and Laboratory (4h/w) Period : Spring ECTS Credits : 7 Recommended reading: Wagner, Biopharmaceutics; M. Gibaldi, Biopharmaceutics and Clinical Pharmacokinetics

Contents : Introduction to Biopharmaceutics. Bioavailability and Bioequivilance. Disintegration, Absorption, Distribution, Protein binding, Clearance. Routes of Administration, Drug dosage forms. Introduction to Pharmacokinetics and Clinical Pharmacokinetics. Pharmacokinetic Models. Disposition and Absorption kinetics (Intravenous dose, Constant rate, Intraveous infusion, Extravascular dose, Metabolite Kinetics). Therapeutic regimens. Individualization. Good Clinical Proactices.

ECTS Course code : PDU-511 Title : Molecular Pharmacology Lecturers : E. Papadimitriou and A. Papapetropoulos Type : Lectures (4h/w) and laboratory (4h/w) Period : Fall ECTS credits : 8

Contents : Introduction to Molecular Pharmacology: Importance and scope. Mechanisms of action of drugs. Classification of drug receptors and binding of drugs. Dose-response studies. Receptors for neurotransmitters and hormones. Receptors that are linked to ion channels and receptors that are coupled to G proteins. Signal transduction mechanisms. Receptor desensitization/down-regulation and adaptation to long-term drug treatment. Drug tolerance and dependence. Quantitative analysis of drug-receptor interactions. Analysis and information derived from signal transduction studies.

Examples of drugs from several groups, such as drugs of the cardiovascular and nervous systems, antiinflamatory and drugs that affect blood functions. Emphasis is given on the mechanisms of actions and the adverse effects of these drugs.

Laboratory course: Tissue and cell isolation and use in pharmacological studies. Dose-response curves. Isolation of cell membranes, in order to use them in binding studies of drugs to their receptor. Tissue homogenization and membrane isolation, in order to use them in binding studies of drugs to their receptor. Total protein determination. Binding studies of labeled ligand to the corresponding receptor in vitro. Specific and non specific binding. Determination of the chemical affinity. Scatchard analysis. Determination of the dissossiation constant and the number of receptors.

ECTS Course Code : PDG-512 Title : Practical Pharmacy I Lecturers : S. Nikolaropoulos, Assist. Professor; G. Pairas, Assist. Professor; K. Avgoustakis, Assist. Professor Type : Laboratory (10h/w) Period : Fall ECTS credits : 10 Courses and training in selected open to the public pharmacies (locally), hospital pharmacies (city of Patras) and pharmaceutical industries (Athens area). Lectures are supported by members of the department and specialists from the above fields.

49 ECTS Cource code : PDU-513 Title : Diploma Work I Lecturer : Faculty Member Type : Period : Fall ECTS credits : 14

Content: Students will select a faculty member and under his quidance they will work on a small research project.

ECTS Course code : PDU-515 Title : Chemistry and Technology of Cosmetics Lecturer : K. Avgoustakis Type : Lectures (2h/w) Period : Spring ECTS Credits :5

Contents : The skin and skin products. The nails and nail products. The hair and hair products. The teeth and dental products. Product ingredients, manufacture and quality control. Cosmetics and Fragrances. Regulations on Cosmetics.

ECTS Cource code : PDU-521 Title : Radiopharmaceuticals Lecturer : Antimissiaris, Assoc. Professor; K. Avgoustakis, Assist. Professor; P. Klepetsanis, Assist. Professor Type : Lectures (5h/w) Period : Spring ECTS Credits :5

Contents : Basic science of Nuclear Medicine : Basic Physics, Radioactive Decay, Measurements and Instrumentation, Radiation and Biology, Radiation dosimetry, protection and regulations. Fundamentals of Nuclear Pharmacy : Production of radionuclides, Radionuclide generators, Design of new pharmaceuticals, quality control of radiopharmaceuticals, Radiolabeling of compounds, characteristics of specific radiopharmaceuticals and uses of pharmaceuticals in Nuclear Medicine.

ECTS Course Code :PDG-522 Title :Practical Pharmacy II

50 Lecturers : S. Nikolaropoulos, Assist. Professor; G. Pairas, Assist. Professor; K. Avgoustakis, Assist. Professor Type :Laboratory (10h/w) Period :Spring ECTS credits : 10 Courses and training in selected open to the public pharmacies (locally), hospital pharmacies (city of Patras) and pharmaceutical industries (Athens area). Lectures are supported by members of the department and specialists from the above fields.

ECTS Cource code : PDU-523 Title : Diploma Work II Lecturer : Faculty Member Type : Laboratory Period : Spring ECTS credits : 12

Content: Continuation of the Diploma work I.

51 GRADUATE STUDIES PROGRAMME

Specializations Offered

A. Industrial Pharmaceutics and Drug Analysis (PDG-Α) B. Pharmaceutical Chemistry-Natural Products: Design, Synthesis and Analysis of Bioactive Compounds (PDG-Β) C. Molecular Pharmacology – Clinical Pharmacy (PDG-C) D. Pharmaceutical Biotechnology and Biomedicine/Biomedical Sciences (PDG- D) E. Pharmaceutical Marketing (PDG-Ε)

List of Graduate Courses

A. Industrial Pharmaceutics and Drug Analysis PDG-Α01 Pharmaceutical Technology PDG-Α02 Industrial Pharmaceutics PDG-Α03 Physical Pharmacy PDG-Α04 Biopharmaceutics and Pharmacokinetics PDG-Α05 Advanced Industrial Pharmaceutics PDG-Α06 Systems of Controlled Administration and Targeting of Drugs PDG-Α07 Immunonalytical and Molecular Methods of Diagnosis PDG-Α08 Quality Control in Pharmaceutical Technology PDG-Α09 Determination of Drugs in Biological Fluids PDG-Α10 Advanced Technology of Solid Form Drugs PDG-Α11 Advanced Technology of Liquid Form Drugs PDG-Α12 Advanced Technology of Semi-Solids Form Drugs and Systems of Dispersion PDG-Α13 Clinical Pharmacokinetics PDG-Α14 Statistical Methods in Pharmaceutics PDG-Α15 Laws and Regulations of Production, Advertisment and Vending of Drugs, Diagnostics and other Health Products PDG-Β13 Analytical Spectroscopy PDG-Β14 Electroanalytical Techniques PDG-Β16 Modern Techniques in Instrumental Analysis PDG-D09 Conformation studies of Macromolecules

B. Pharmaceutical Chemistry-Natural Products: Design, Synthesis and Analysis of Bioactive Compounds PDG-Β01 Organic Chemistry: Organic Reactions Mechanisms - Stereochemistry PDG-Β02 Organic Chemistry: Protective Groups – Combinatorial Chemistry PDG-Β03 Isolation and Identification of Natural Products PDG-Β04 Chemistry of Aminoacids, Peptides and Proteins PDG-Β05 Current Approaches in Pharmaceutical Chemistry PDG-Β06 Total Synthesis of Natural Products PDG-Β07 Drug Design PDG-Β08 Bioinorganic Chemistry PDG-Β12 Computer Applications in Pharmacy PDG-Β13 Analytical Spectroscopy PDG-Β14 Electroanalytical Techniques

52 PDG-Β15 Separation Techniques PDG-Β16 New Techniques in Instrumental Analysis PDG-D09 Conformational studie of Macromolecules

C. Molecular Pharmacology – Clinical Pharmacy PDG-C01 General Principles in Pharmacology PDG-C02 Biochemical Basis of Drug Action PDG-C03 Advanced Molecular Pharmacology PDG-C04 In vivo and in vitro studies on the mechanism of drug action PDG-C05 Pathophysiology and pharmacology of angiogenesis PDG-C06 Approaches for the development of new drugs PDG-C07 Signal transduction mechanisms in the CNS PDG-C08 Molecular basis of behavior PDG-C09 Clinical Pharmacy I PDG-C10 Clinical Pharmacy II PDG-C11 Introduction to Pathology

D. Pharmaceutical Biotechnology and Biomedicine PDG-D01 Pharmaceutical Biotechnology PDG-D02 Advanced Molecular Biotechnology PDG-D03 Laboratory Training in Molecular Biology-Biotechnology Techniques PDG-D04 Molecular Diagnostics PDG-D05 Advanced Immunobiology PDG-D06 Molecular Biology of Cancer PDG-D07 Introduction into Biomedical Research PDG-D08 Bioinformatics PDG-D09 Conformational studies of Macromolecules PDG-D10 Protein Biochemistry

E. Pharmaceutical Marketing PDG-Ε01 Research and Development in Pharmaceutical Industry PDG-Ε02 Design of Marketing Strategies PDG-Ε03 Communication Strategies PDG-Ε04 Management in Finance PDG-Ε05 Principles of Business Administration PDG-E06 Introduction into Financial Studies PDG-E07 Sales PDG-E08 Pharmacoeconomy PDG-E11 Pharmaceutical Technology PDG-E12 General principles of Pharmacology PDG-E13 Drug Desing and Development PDG-E14 Pharmaceutical Marketing Diploma Research

Diploma Research Credits (for Specializations A-D) PDG-Υ01 Diploma Research Ι PDG-Υ02 Diploma Research ΙΙ PDG-Υ03 Diploma Research ΙΙΙ PDG-Υ04 Diploma Research ΙV

53 COURSE SUMMARY TABLE A. Industrial Pharmaceutics and Drug Analysis First year graduate students must select four of the following offered courses during the first two semesters and research projects -Y01 and -Y02. Second year students must attend two more courses and research projects -Y03 and -Y04. Total credits per semester are 30.

FALL 06 SPRING 07 PDG-A01 3(L:h/w) 10 PDG-A02 3(L:h/w) 10 PDG-A03 3(L:h/w) 10 PDG-A04 3(L:h/w) 10 PDG-A08 3(L:h/w) 10 PDG-A05 3(L:h/w) 10 PDG-A10 3(L:h/w) 10 PDG-A06 3(L:h/w) 10 PDG-A11 3(L:h/w) 10 PDG-A14 3(L:h/w) 10 PDG-A12 3(L:h/w) 10 PDG-A13 3(L:h/w) 10 PDG-B13 3(L:h/w) 10