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Lieferung: frei Haus Bestellung auf Rechnung SZABO-SCANDIC Lieferung: € 10,- HandelsgmbH & Co KG Erstbestellung Vorauskassa Quellenstraße 110, A-1100 Wien T. +43(0)1 489 3961-0 Zuschläge F. +43(0)1 489 3961-7 [email protected] • Mindermengenzuschlag www.szabo-scandic.com • Trockeneiszuschlag • Gefahrgutzuschlag linkedin.com/company/szaboscandic • Expressversand facebook.com/szaboscandic SAN TA C RUZ BI OTEC HNOL OG Y, INC . (C-9): sc-393188

BACKGROUND APPLICATIONS Cell cycle progression is subject to arrest at the mitotic spindle assembly MAD2 (C-9) is recommended for detection of MAD2 of mouse, rat and checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitot ic human origin by Western Blotting (starting dilution 1:100, dilution range arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total mutated MAD2 do not undergo mitotic arrest in response to incorrect spin dle (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution fiber assembly, which results in missegregation and eventual cell death. A range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution breast carcinoma cell line with reduced MAD2 expression, T47D, was shown range 1:30-1:3000). to complete mitosis in the presence of nocodazole, an inhibitor of mitotic MAD2 (C-9) is also recommended for detection of MAD2 in additional spindle assembly. MAD2 is localized to unattached during species, including equine, bovine and porcine. prometaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates binding to the spindle fibers. Human MAD2 Suitable for use as control antibody for MAD2 siRNA (h): sc-35837, MAD2 has also been shown to associate with (IR), but not IGFIR, siRNA (m): sc-35838, MAD2 shRNA Plasmid (h): sc-35837-SH, MAD2 shRNA implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 Plasmid (m): sc-35838-SH, MAD2 shRNA (h) Lentiviral Particles: sc-35837-V homolog, is required for the execution of the mitotic checkpoint monitoring and MAD2 shRNA (m) Lentiviral Particles: sc-35838-V. the kinetochore-spindle attachment process and if the process is not com - Molecular Weight of MAD2: 25 kDa. plete, MAD2B delays the onset of anaphase. Positive Controls: Jurkat nuclear extract: sc-2132, K-562 nuclear extract: REFERENCES sc-2130 or HeLa nuclear extract: sc-2120. 1. Murray, A.W. 1992. Creative blocks: cell-cycle checkpoints and feedback RECOMMENDED SUPPORT REAGENTS controls. Nature 359: 599-604. To ensure optimal results, the following support reagents are recommended: 2. Glotzer, M. 1996. Mitosis: don’t get mad, get even. Curr. Biol. 6: 1592- 1594. 1) Western Blotting: use m-IgG κ BP-HRP: sc-516102 or m-IgG κ BP-HRP (Cruz 3. Chen, R.H., et al. 1996. Association of spindle assembly checkpoint com - Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ ponent XMAD2 with unattached kinetochores. Science 274: 242-246. Molecular Weight Standards: sc-2035, UltraCruz ® Blocking Reagent: sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopre- 4. Li, Y., et al. 1996. Identification of a human mitotic checkpoint : cipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). hsMAD2. Science 274: 246-248. 3) Immunofluorescence: use m-IgG κ BP-FITC: sc-516140 or m-IgG κ BP-PE: 5. O’Neill, T.J., et al. 1997. Interaction of MAD2 with the carboxyl terminus sc-516141 (dilution range: 1:50-1:200) with UltraCruz ® Mounting Medium: of the Insulin receptor but not with the IGFIR. Evidence for release from sc-24941 or UltraCruz ® Hard-set Mounting Medium: sc-359850. the Insulin receptor after activation. J. Biol. Chem. 272: 10035-10040. 6. Liu, S.T., et al. 2003. Human CENP-I specifies localization of CENP-F, DATA and MAD2 to kinetochores and is essential for mitosis. Nat. Cell Biol. 5: 341-345. ABCDE AB 50K– 55K– 43K– CHROMOSOMAL LOCATION 34K– 34K– Genetic locus: MAD2L1 (human) mapping to 4q27; Mad2l1 (mouse) mapping < MAD2 to 6 C1. 22K– < MAD2 23K– 19K– SOURCE MAD2 (C-9) is a mouse monoclonal antibody specific for an epitope mapping MAD2 (C-9): sc-393188. Western blot analysis of MAD2 (C-9): sc-393188. Western blot analysis of MAD2 MAD2 expression in Jurkat (A), K-562 (B), HeLa (C), expression in Hep G2 (A) and MCF7 (B) whole cell between amino acids 178-205 at the C-terminus of MAD2 of human origin. BJAB (D) andMEG-01 ( E) nuclear extracts. lysates.

PRODUCT SELECT PRODUCT CITATIONS

Each vial contains 200 µg IgG 1 kappa light chain in 1.0 ml of PBS with < 0.1% 1. Zhang, X., et al. 2016. Mps1 kinase regulates tumor cell viability via its sodium azide and 0.1% gelatin. novel role in mitochondria. Cell Death Dis. 7: e2292. Blocking peptide available for competition studies, sc-393188 P, (100 µg 2. Maddalena, F., et al. 2017. TRAP1 protein signature predicts outcome in pep tide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% stabilizer human metastatic colorectal carcinoma. Oncotarget 8: 21229-21240. protein). STORAGE RESEARCH USE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of For research use only, not for use in diagnostic procedures. shipment. Non-hazardous. No MSDS required.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com