Anti-hIL-29-IgG Neutralizing monoclonal antibody against human 29 Catalog # mabg-hil29-3

For research use only, not for diagnostic or therapeutic use Version # 15C23-MM

PRODUCT INFORMATION DESCRIPTION Content: 3 x 100 µg purified anti-hIL-29-IgG antibody, provided Anti-hIL-29-IgG is a monoclonal antibody against human azide-free and lyophilized interleukin 29 (hIL-29). This antibody has been selected for its ability Target: natural and recombinant human interleukin-29 (IL-29) to efficiently neutralize the biological activity of hIL-29 . Specificity: Reacts with human IL-28A and human IL-28B. Anti -hIL -29 -IgG is produced in hybridomas and purified by affinity No cross -reactivity with mouse IL-28A or mouse IL-28B. chromatography. Note: An active IL-29 is absent in mice. Clone: 6A11 APPLICATIONS Isotype: Mouse IgG2a Anti-hIL-29-IgG is a neutralizing antibody, it blocks hIL-29-induced Immunogen: Human IL-29 protein expressed in Swiss mice cellular activation. Other applications have not been tested. following DNA immunization m Formulation: 0.2 m filtered solution in a sodium phosphate buffer Neutralization with glycine, saccharose and stabilizing agents The exact concentration of antibody required to neutralize hIL-29 Antibody resuspension activity is dependent on the concentration, cell type and Add 1 ml of sterile water per vial to obtain a concentration of 0.1 mg/ml. growth conditions. InvivoGen has determined the neutralization dose Storage for this antibody using recombinant hIL-29 and HEK -Blue ™ IFN -a/b - Product is shipped at room temperature. Store lyophilized antibody cells . These cells were generated by stable transfection of HEK293 at -20 °C. Product is stable for at least 1 year. cells with the human STAT2 and IRF9 to obtain a fully active - Reconstituted antibody is stable for 1 month when stored at 4 °C and type I IFN signaling pathway. They were further transfected with a for 1 year when aliquoted and stored at -20 °C. Avoid repeated SEAP (secreted embryonic alkaline phosphatase) reporter gene under freeze-thaw cycles. the control of the IFN- α/β inducible ISG54 promoter . Quality control Anti-hIL-29-IgG (50 ng-1 mg/ml) and a negative control antibody - This product has been validated for neutralization. (e.g. Mouse IgG2a control which targets E. coli β-galactosidase) were - The absence of bacterial contamination (e.g. lipoproteins and endotoxins) ™ ™ incubated with recombinant hIL-29 at 10 ng/ml for 30 min prior to the has been confirmed using HEK-Blue TLR2 and HEK -Blue TLR4 cells. addition of the HEK-Blue ™ IFN- a/b cells . Neutralization of IL -29- induced signaling by anti-hIL-29-IgG was determined after a 24-hour BACKGROUND incubation by assessing SEAP production using QUANTI -Blue ™, a Interleukin-29 (IL-29) is a member of the type III (IFN SEAP detection reagent. QUANTI-Blue ™ turns blue following lambda) cytokine family, which comprises three members: IL-28A, cytokine stimulation but remains pink if neutralization occurs. SEAP IL -28B and IL-29. It should be noted that in the mouse genome IL -29 is levels can be assessed by the naked eye or spectrophotometrically by a . Type III IFNs exhibit several common features with type I reading the optical density at 620 -655 nm. IFNs: antiviral activity and antitumor activity 1, 2 . In fact, it has been demonstrated that IL-29 works together with type I IFN to promote an RELATED PRODUCTS antiviral response to hepatitis 3. IL-29 is produced by and dendritic cells in response to viral infection and stimulation with toll -like Product Catalog Code receptor (TLR) ligands 4. IL-29 exerts its action following binding to a heterodimeric protein complex composed of two subunits, IL-28 HEK-Blue ™ IFN- a/b Cells hkb-ifnab receptor alpha and IL-10 receptor beta, leading to signaling through the Mouse IgG2a Control mabg2a-ctrlm Jak/Stat pathway and inducing the expression of IFN-stimulated genes. QUANTI-Blue ™ rep-qb1

1. Donnelly RP. & Kotenko SV., 2010. Interferon-lambda: a new addition to an old family. J Interferon Cytokine Res. 30(8):555-64. 2. Li M. et al., 2009. Interferon- ls: the modulators of antivirus, antitumor, and immune responses. J. Leukoc. Biol., 86:23-32. 3. Pagliaccetti NE. et al., 2008. Interleukin-29 functions cooperatively with interferon to induce antiviral and inhibit hepatitis C replication. J Biol Chem. 283(44):30079-89. 4. Wolk K. et al., 2008. Maturing dendritic cells are an important source of IL-29 and IL-20 that may cooperatively increase the innate immunity of keratinocytes. J. Leukoc Biol. 83(5):1181-93.

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