Polyclonal Antibody to PDE6B

AP10244PU-N OriGene Technologies Inc. OriGene EU

Acris Antibodies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

Polyclonal Antibody to PDE6B - Aff - Purified Alternate names: 5'-cyclic phosphodiesterase subunit beta, GMP-PDE beta, PDE6 beta, PDEB, Phosphodiesterase 6 beta, Phosphodiesterase 6B, Phosphodiesterase-6B cGMP-specific, Rod cGMP-specific 3' Catalog No.: AP10244PU-N Quantity: 0.1 mg Concentration: 0.5-0.75 mg/ml (lot. specific) Background: The retinal cone and rod photoreceptor cells can directly stimulate and response by photons of the light. Upon light stimulation these cells undergo a highly regulated cascade of events that leads to changes in the photoreceptor membrane potentials and subsequent neurotransmitter release. The key component of photo transduction in retina is retinal specific cGMP-dependent phosphodiesterases type 6 (PDE6s). These PDEs convert cGMP in to GMP when activated by the G-protein transducin and caused a closer of the cGMP gated ion channels, reduced Na+ and Ca++ influx and hyperpolarization of the photoreceptor cells. Rodhopsin and PDE6 are also colocalized in the internal membraneous disk of the photoreceptor cells allowing most efficient transfer of light signal from one protein to other. The retinal phosphodiesterses are multimeric proteins consisting of catalytic and inhibitory subunits. The large alfa and beta (a and b) subunits of the rod and cone specific PDE6 dimerises to form the catalytic complex of the PDE6 isozyme. The smaller gamma subunit serves as an inhibitor for the rod and cone phosphodiesterses (1). There are 3 subtypes of PDE6 found in rod and cone cells, PDE6a, PDE6b and and inhibitory PDE6g. The rod and cone PDEs differ significantly in subunit compositions, sensitivity to transducin, and cGP binding properties (2). The PDE6a and beta forms have 2 non catalytic binding cGMP sites; the cone biding site has significantly lower Ki for cGMP that the rod enzymes. There is significant homology between a and b subunits in the catalytic and GAF binding domains. Both PDE6a and PDE6b are post-translationally modified, PDE6a is farnsylated and beta subunit is gernylgernylated (3). Generally, these modifications will allow proteins to be anchored to the membranes, incase of PDE6, despite 2 lipid modifications the PDE can easily be removed form membranes by detergent free hypotonic buffers. The PDE6 can be localized in both membrane and cytosolic compartment, the cytosolic PDE6 has been show to be associated with a 17 kDa delta subunit (4). The role of delta subunit in PDE6 functioning is not fully understood. It been hypothesized that delta subunit might be playing a role in PDE6 solubility in the photoreceptor cells. The PDE6b is a 103-105 kDa protein (858 amino acids) with 2 GAF and one PDEase catalytic domain. Uniprot ID: P35913 NCBI: NP_000274 GeneID: 5158

For research and in vitro use only. Not for diagnostic or therapeutic work. OG/20130514 Material Safety Datasheets are available at www.acris-antibodies.com or on request. Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com 1 / 2 AP10244PU-N: Polyclonal Antibody to PDE6B - Aff - Purified Host: Rabbit Immunogen: This antibody was generated against peptide from unique sequence on the PDE6 beta gene. Format: State: Liquid purified Ig fraction. Purification: Antigen-Sepharose Affinity Chromatography. Buffer System: Stabilization buffer. Applications: Western Blot (1/500). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody detects a 103 kDa PDE6 beta subunit proteins and does not cross react with other PDE6 subunits or other PDE family members. Species: Human, Bovine, Mouse and Rat. Other species not tested. Add. Information: Antigenic blocking peptide (AP10244CP-N) for PDE6 beta is also available separately. Storage: Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Florio S. K., Prusti R.K., and Beavo J. A., J. Biol. Chem. 271; 24036-24047; 1996. 2. Charbonneau H., Prusti R. K., et al., Proc. Natl. Aad. Sci. USA 87; 288-292; 1990. 3. Cook T. A., Ghomashchi F., Gelb M. H., et. al., J. Biol. Chem. October 26, 2000. 4. Gillespie P. G., and Beavo J. A. J. Biol. Chem. 263; 8133-8141; 1988.