SEKK SUMMARY STATISTICS EQA round: NF2/19 - Peripheral Blood Smears - Photos Dead line (EQA round closed): 27.09.2019 Professional Czech Haematological Society supervision: National Reference Laboratory for Haematology Key: >>> ... marks correct (expected) result > ... marks conditionally correct (acceptable) result

Photo 1 Object no.1WBC and other nucleous elements - types of cells >>> 1 Blast 1,9 % >>> 43 Promyelocyte 81 % 1 Neutrophil myelocyte 1,9 % 6 Neutrophil metamyelocyte 11 % 1 Neutrophil bar 1,9 % 1 Monocyte 1,9 % WBC - morphology 8 No changes 15 % 1 Hypergranulation/ 1,9 % 1 Giant bands and metamyelocytes 1,9 % 4 Auer rods 7,5 % 1 Atypical/reactive monocytes 1,9 % 2 Nucleocytoplasmatic asynchrony 3,8 % 1 Vacuolisation 1,9 % >>> 38 Leukemic promyelocyte 72 % Object no.2WBC and other nucleous elements - types of cells >>> 2 Blast 3,8 % >>> 44 Promyelocyte 83 % 1 Neutrophil myelocyte 1,9 % 3 Neutrophil metamyelocyte 5,7 % 1 Neutrophil segment 1,9 % 1 Monocyte 1,9 % 1 1,9 % WBC - morphology 4 No changes 7,5 % 1 Hypergranulation/toxic granulation 1,9 % 1 Atypical/reactive monocytes 1,9 % 5 Nucleocytoplasmatic asynchrony 9,4 % 9 Vacuolisation 17 % >>> 40 Leukemic promyelocyte 75 % RBC - morphology 8 Normocytosis 15 % 3 5,7 % 2 3,8 % 1 1,9 % 16 Spherocytes 30 % 8 Stomatocytes 15 % 1 Drepanocytes 1,9 % >>> 50 (and other fragmentocytes) 94 % 1 Polychromasia 1,9 % >>> 31 Hypochromia 58 % 6 11 % 2 Howell-Jolly bodies 3,8 % 3 formation 5,7 % 6 Anisochromia 11 % > 16 Knizocytes 30 % > 16 Ovalocytes 30 % Platelets - morphology 8 No changes 15 % 11 Anisocytosis 21 % >>> 41 Platelets hypo-/agranulation 77 % Photo 2 Object no.1WBC and other nucleous elements - types of cells >>> 53 Basophiles 100 % WBC - morphology >>> 45 No changes 85 % 2 Hypergranulation/toxic granulation 3,8 %

Printed 10.10.2019, summary evaluation of the results should be found at http://www.sekk.cz Page: 1 SEKK NF2/19 - Peripheral Blood Smears - Photos Photo 2 Object no.1WBC - morphology > 5 Vacuolisation 9,4 % Object no.2WBC and other nucleous elements - types of cells >>> 53 Neutrophil segment 100 % WBC - morphology 3 No changes 5,7 % 2 Hypergranulation/toxic granulation 3,8 % 2 Hypogranulation/agranulation 3,8 % 1 Hypersegmented granulocytes 1,9 % 1 Hyposegmentation or pseudo Pelger anomaly 1,9 % 1 Cytoplasmatic fragments 1,9 % >>> 49 Vacuolisation 92 % RBC - morphology 1 No changes 1,9 % 2 Normocytosis 3,8 % 5 Macrocytosis 9,4 % >>> 33 Anisocytosis 62 % 31 Spherocytes 58 % >>> 53 Stomatocytes 100 % 1 Dacryocytes 1,9 % 4 7,5 % 2 Target cells 3,8 % 4 Schistocytes (and other fragmentocytes) 7,5 % 1 Basophilic stippling 1,9 % 1 Rouleaux formation 1,9 % 4 Anisochromia 7,5 % > 14 Knizocytes 26 % > 18 Ovalocytes 34 % Platelets - morphology 25 Large platelets 47 % 1 Small platelets 1,9 % >>> 51 Anisocytosis 96 % > 10 Platelet aggregates 19 % 1 Platelet satellitism 1,9 % >>> 42 Platelets hypo-/agranulation 79 % Photo 3 Object no.1WBC and other nucleous elements - types of cells >>> 53 Lymphocyte 100 % WBC - morphology > 31 No changes 58 % > 10 Lymphocytes - reactive forms 19 % 6 "Hairy" lymphocytes 11 % 6 Lymphocytes - atypical forms 11 % RBC - morphology 3 Normocytosis 5,7 % 5 Macrocytosis 9,4 % > 16 Anisocytosis 30 % 8 Poikilocytosis 15 % >>> 48 Eliptocytes 91 % 18 Spherocytes 34 % 3 Stomatocytes 5,7 % > 26 Dacryocytes 49 % 6 Acanthocytes 11 % 5 9,4 % > 25 Schistocytes (and other fragmentocytes) 47 % 2 Polychromasia 3,8 % > 27 Howell-Jolly bodies 51 % 13 25 % 1 Knizocytes 1,9 % >>> 45 Ovalocytes 85 % Platelets - morphology > 24 No changes 45 % 6 Anisocytosis 11 % > 27 Platelets hypo-/agranulation 51 % Photo 4 Object no.1WBC and other nucleous elements - types of cells >>> 53 Neutrophil segment 100 % WBC - morphology >>> 38 No changes 72 % 8 Hypergranulation/toxic granulation 15 % 1 Hypogranulation/agranulation 1,9 % 5 Hyposegmentation or pseudo Pelger anomaly 9,4 % 1 Cytoplasmatic fragments 1,9 % RBC - morphology 1 1,9 % 12 Macrocytosis 23 % Printed 10.10.2019, summary evaluation of the results should be found at http://www.sekk.cz Page: 2 SEKK NF2/19 - Peripheral Blood Smears - Photos Photo 4 RBC - morphology >>> 43 Anisocytosis 81 % 9 Poikilocytosis 17 % >>> 22 Eliptocytes 42 % 9 Spherocytes 17 % > 32 Stomatocytes 60 % 1 Dacryocytes 1,9 % 1 Acanthocytes 1,9 % 10 Echinocytes 19 % > 31 Target cells 58 % > 43 Schistocytes (and other fragmentocytes) 81 % 30 Polychromasia 57 % >>> 44 Hypochromia 83 % 6 Howell-Jolly bodies 11 % 3 Rouleaux formation 5,7 % 1 Pappenheimer bodies 1,9 % 5 Anisochromia 9,4 % 9 Knizocytes 17 % >>> 49 Ovalocytes 92 % Platelets - morphology 8 No changes 15 % >>> 28 Anisocytosis 53 % >>> 32 Platelets hypo-/agranulation 60 % Evaluation of the results - scoring system NF2/19 Photo 1 Photo 2 Photo 3 Photo 4 Maximal achievable score: 35 40 15 40 Successful participants (success 60 % and more): 40 (it is 75 %) 50 (it is 94 %) 46 (it is 87 %) 34 (it is 64 %) Minimal success in this round: -5,7 % 52,5 % 40,0 % 37,5 % Maximal success in this round: 100,0 % 100,0 % 100,0 % 100,0 %

Number of participants: 53 in all 4 photos: 23 (it is 43 %) in 3 photos: 19 (it is 36 %) Number of participants that succeeded: in 2 photos: 10 (it is 19 %) in 1 photo: 1 (it is 2 %) in no photo: 0 (it is 0 %)

Printed 10.10.2019, summary evaluation of the results should be found at http://www.sekk.cz Page: 3