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Vol. 24, No. 4, 2010 SUBSCRIPTION RATES Annual Subscription Editor US $ 50.00 (Postage Extra) K.L Indian Journal of Gerontology Indian Journal of (A quarterly journal devoted to research on ageing) Gerontology ISSN : 0971-4189 a quarterly journal devoted to research on ageing Vol. 24, No. 4, 2010 SUBSCRIPTION RATES Annual Subscription Editor US $ 50.00 (Postage Extra) K.L. Sharma UK £ 30.00 (Postage Extra) Rs. 400.00 Libraries in India Free for Members EDITORIAL BOARD Biological Sciences Clinical Medicine Social Sciences Financial Assistance Received from : B.K. Patnaik S.D. Gupta Uday Jain ICSSR, New Delhi P.K. Dev Shiv Gautam N.K. Chadha S.P. Sharma P.C. Ranka Ishwar Modi CONSULTING EDITORS A.V. Everitt (Australia), Harold R. Massie (New York), P.N. Srivastava (New Delhi), R.S. Sohal (Dallas, Texas), Printed in India at : A. Venkoba Rao (Madurai), Sally Newman (U.S.A.) Lynn McDonald (Canada), L.K. Kothari (Jaipur) Aalekh Publishers S.K. Dutta (Kolkata), Vinod Kumar (New Delhi) M.I. Road, Jaipur V.S. Natarajan (Chennai), B.N. Puhan (Bhubaneswar), Gireshwar Mishra (New Delhi), H.S. Asthana (Lucknow), Arun. P. Bali (Delhi), R.S. Bhatnagar (Jaipur), H.L. Dhar (Mumbai), Arup K. Benerjee (U.K.), Indira J. Prakash (Bangalore), Yogesh Atal (Gurgaon), V.S. Baldwa (Jaipur), P. Uma Devi (Kerala) Typeset by : MANAGING EDITORS Sharma Computers, Jaipur A.K. Gautham & Vivek Sharma Phone : 2621612 CONTENTS YOU ARE INVITED TO JOIN US S.No. Page No. We are Working to Protect the Rights and Social Welfare of the Elderly Indian Gerontological Association (Registration No 212/ 1968) is an independent 1. Lipase Activity During Ageing of Brinjal Shoot and grassroot non-profit organization based in Jaipur (Rajasthan). Our efforts empower Fruit Borer, Leucinodes orbonalis (Guenee) 413-420 and support the underprivileged elderly in rural and urban communities. Ramesh M. Gejage and Manisha R. Gejage We strive to ensure social justice and welfare for people over 60, focusing on those elders who are the most disadvantaged such as elderly women. We protect 2. A Study of ENT and Head Neck Diseases in Geriatric the civil liberties of elderly citizens as a part of the struggle for individual rights Population at Tertiary Care Hospital 421-433 and social progress in India. Darshan Goyal and Ritu Currently, the elderly community comprises approximately 10% of the total population of India. This number will increase to nearly 16% within the next twenty years. 3. Perceptions and Risk Factors for Oral Cancers in Neglected and abandoned by society and sometimes their own families, the Rural Elderly 434-444 elders are increasingly subject to conditions of disease and poverty. They Pratibha Dabas and M.M. Angadi lack access to health care, and often face serious discrimination as well as physical and emotional abuse. 4. The Relationship of Locus of Control, Sociability and As a public interest group, we work for and with the elderly to protect their Impulsivity with Age 445-451 rights and access to a better quality of life. We seek to both empower and serve by working directly with rural communities. By facilitating the growth of Uma Gupta, Ram Narayan and B.S. Gupta citizen’s groups, raising public awareness on ageing, promoting public action and participation, and advocating public policy changes, Indian Gerontological Association 5. Psychoclinical Constraints of Morbidity among the hopes to alter the current trends in elder relations for the better. 80+ vis-a-vis Prospects of Healthy Ageing 452-461 Our work includes : Bhaweshwar Singh @ Community Centers for the Elderly that Offer Communal Support and Interaction 6. Ageing and Vulnerability to Criminality in Nigeria 462-481 Olabisi James AYODELE @ Training on Legal Rights by Offering the Elderly Practical Knowledge on Their Rights 7. Ageing and Family Support of Elderly in South India 482-500 @ Public Helpline for the Elderly that Offers Legal Referrals and Assistance A.K. Ravishankar (Helpline : 0141-2624848). @ Public Accessibility for the Elderly Advocating More Available Access to the 8. Family in Transition and Challenges for Elderly Persons 501-508 Public Sphere Asiya Nasreen @ Use of various forms of media to Raise Public Awareness on Elder Rights @ Counselling and Helping elderly to Relieve Psychological Stress and Depression 9. Resisting Commonsense Assumptions in Caregivers @ Elder Women’s Cooperatives that Provide Grants and Assistance to Elderly Talk 509-525 Women Isabella Paoletti @ Public Awareness Raising to Promote Public Action for Helping Disadvantaged Elderly 10. Fragile Flames : Challenges Experienced by Aged Destitute Women in Vrindavan 526-535 @ Field Study of Rural Areas to Analyze Challenges Faced by Ageing Rural Neetu Rana and Gireshwar Misra Population Our plan of action includes:- For Our Readers 536 @ Campaign for Elder Rights @ Campaign Against Elder Abuse especially toward Elderly Women @ Training of Social Workers and Caregivers @ Capacity Building of Civil Servants or organizations Working on Ageing @ Research & Publication. 414 Indian Journal of Gerontology Indian Journal of Gerontology organisms, they are instrumental in the deposition and mobilization of 2010, Vol. 24, No. 4. pp. 413 -420 the fat. They are also involved in the metabolism of intracellular lipids Pol and Salunkhe (2001b). Lipase Activity During Ageing of Brinjal Shoot Many attempts have been made to investigate lipase activity in few insect species (Nandanan et al.,1973; Price, 1975; and Fruit Borer, Leucinodes orbonalis (Guenee) Radha Pant and Sharma, 1978; Hoffman and Downer,1979; Male and Story, 1981; Wheeler et al., 1984; Soulages and Wells,1994 ; Pistillo et Ramesh M. Gejage and Manisha R. Gejage* al., 1998; Pol and Sawant, 1999; Arreguin et al., 2000; Pol and Department of Zoology, K.R.P. Kanya Mahavidyalaya, Islampur Tal. Walwa Salunkhe, 2001a, 2001b; Ponnuvel et al., 2003; Gejage and Awate, Dist. Sangli-415 409 (MS) 2009; Horne et al., 2009).The information on lipase activity during ageing *Department of Zoology, Vishwasrao Naik Mahavidyalaya, Shirala Tal. of female adult brinjal shoot and fruit borer, L. orbonalis (Guenee) is Shirala, Dist. Sangli-415 408 (MS) rather scanty. In the present investigation, an attempt has been made to measure ABSTRACT lipase activity during ageing of female adult of brinjal shoot and fruit The brinjal shoot and fruit borer, Leucinodes orbonalis (Guenee) borer, L. orbonalis (Guenee) which is mainly concerned with release is an important pest of brinjal in South Asia. The enzyme lipase in of energy for the development of eggs (oogenesis) and oviposition. female adult revealed optimum pH 8.2, incubation time 30 minutes, temperature 370 C, enzyme concentration 1 % and substrate MATERIALS AND METHODS concentration 5 %. A gradual increase in lipase activity was The culture of L. orbonalis (Guenee) was maintained in the observed from 1-day old female adults to 3-day old adults and Laboratory on the natural food of brinjal fruit (Pol and Gejage, 2002). decreases from 3-day old adults to 5-day old adults. The maximum Female from developmental stages 1 day to 5 day old were taken for lipase activity was observed in 3- day old female adults. In male the study of lipolytic activity. adult, the lipase activity was 1.0548 folds more than 3-day old female adults. The physiological role of lipase during ageing of For the enzyme preparation, the female moths were isolated. The female adult brinjal shoot and fruit borer, L. orbonalis (Guenee) wings, antennae and legs were removed and cleaned with distilled water, is reported in the present paper. weighed and homogenized in the cold double distilled water using a ground glass mortar and pestle. The homogenate were diluted with Key words: Triacylglycerol lipase, Insect, Female adult, L. orbonalis cold double distilled water so as to get 1% (wt/vol) concentration. Such (Guenee). homogenate were used for the assay of lipolytic activity. The lipase Brinjal shoot and fruit borer, Leucinodes orbonalis (Guenee) is was assayed by the method of Hayase and Tapple (1970). The assay the most destructive pest of brinjal. The young larvae bore into tender system contained 0.25 ml of 5 % substrate dispersed in gum acacia; 1.0 shoots near the growing points into flower buds or into the fruits. The ml of 0.1 M tris-maleate buffer pH 8.2 and 0.25 ml of 1 % (wt/vol) immature fruits are also damaged, which finally leads to the economic enzyme solution in a total volume of 1.5 ml. The incubations were carried loss to the farmers. The adult developmental stage is of 5 days in August out in a shaker with a continuous shaking for 30 minutes in glass stoppered 0 and September. The triacylglycerol lipase is an enzyme which is vessels at 37 C. At the end of the incubation the liberated fatty acids responsible for hydrolysis of triglyceride. Lipolytic enzymes are were measured colorimetrically according to Itaya (1977). indispensable for the biological turnover of lipids. They are required as RESULTS AND DISCUSSION digestive enzymes in the transfer of lipid from one organism to another, that is from plant to animal and from animal to animal. Within the Adult developmental period of L. orbonalis (Guenee) is of 5-days. Lipase activity during ageing of female adult of L. orbonalis (Guenee) Lipase Activity during Ageing 415 416 Indian Journal of Gerontology is shown in figure 1. The adult lipase revealed optimum pH 8.2, incubation synthesize these from proteins and carbohydrates. The study of time 30 minutes, temperature 370 C, enzyme concentration 1 % and hemolymph components as well as of fat body deposits involved in substrate concentration 5 %. A gradual increase in lipase activity was essential metabolic processess related to supply of energy in insects for observed from 1-day old female adults to 3-day old adults but decreased activities such as flight, reproduction, embryogenesis and metamorphosis from 3-day old adults to 5-day old adults. The maximum lipase activity which provide important information about physiology of members of was observed in 3- day old female adults.
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