ARPC1A Antibody Cat. No.: 62-297
ARPC1A Antibody
Flow cytometric analysis of hela cells using ARPC1A ARPC1A Antibody immunohistochemistry analysis in Antibody (bottom histogram) compared to a negative formalin fixed and paraffin embedded human brain tissue control cell (top histogram). FITC-conjugated goat-anti- followed by peroxidase conjugation of the secondary rabbit secondary antibodies were used for the analysis. antibody and DAB staining.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
This ARPC1A antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 157-184 amino acids from the Central region of human ARPC1A.
TESTED APPLICATIONS: Flow, IHC-P, WB
October 2, 2021 1 https://www.prosci-inc.com/arpc1a-antibody-62-297.html For WB starting dilution is: 1:1000
APPLICATIONS: For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
PREDICTED MOLECULAR 42 kDa WEIGHT:
Properties
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by PURIFICATION: dialysis
CLONALITY: Polyclonal
ISOTYPE: Rabbit Ig
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION: batch dependent
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: ARPC1A
ALTERNATE NAMES: Actin-related protein 2/3 complex subunit 1A, SOP2-like protein, ARPC1A, SOP2L
ACCESSION NO.: Q92747
PROTEIN GI NO.: 88984001
GENE ID: 10552
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
ARPC1A is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein ARPC1B. The similarity between these two proteins suggests that they both may function as p41 BACKGROUND: subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.
October 2, 2021 2 https://www.prosci-inc.com/arpc1a-antibody-62-297.html REFERENCES: 1) Laurila,E., Genes Chromosomes Cancer 48 (4), 330-339 (2009)
2) Machesky,L.M., Biochem. J. 328 (PT 1), 105-112 (1997)
ANTIBODIES FOR RESEARCH USE ONLY.
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October 2, 2021 3 https://www.prosci-inc.com/arpc1a-antibody-62-297.html