Genome DNA barcodes and citizen science provoke a diversity reappraisal for the “ring” butterflies of Peninsular Malaysia (Ypthima : Satyrinae: Nymphalidae: Lepidoptera) Journal: Genome Manuscript ID gen-2015-0156.R1 Manuscript Type: Article Date Submitted by the Author: 16-Mar-2016 Complete List of Authors: Jisming-See, Shi-Wei; University of Malaya, Institute of Biological Sciences Sing, Kong-Wah;Draft University of Malaya, Institute of Biological Sciences Wilson, John; University of Malaya, cytochrome c oxidase I, DNA barcode, Satyrinae, taxonomy, Keyword: <i>Ypthima</i> https://mc06.manuscriptcentral.com/genome-pubs Page 1 of 31 Genome DNA barcodes and citizen science provoke a diversity reappraisal for the “ring” butterflies of Peninsular Malaysia ( Ypthima : Satyrinae: Nymphalidae: Lepidoptera) Shi-Wei Jisming-See 1,2 , Kong-Wah Sing 1,2 , John-James Wilson 1,2* 1Museum of Zoology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. 2Ecology and Biodiversity Program, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia. *Corresponding author: John-James Wilson (
[email protected]) Draft https://mc06.manuscriptcentral.com/genome-pubs1 Genome Page 2 of 31 Abstract The “rings” belonging to the genus Ypthima are amongst the most common butterflies in Peninsular Malaysia. However, the species can be difficult to tell apart, with keys relying on minor and often non-discrete ring characters found on the hindwing. Seven species have been reported from Peninsular Malaysia but this is thought to be an underestimate of diversity. DNA barcodes of 165 individuals, and wing and genital morphology, were examined to reappraise species diversity of this genus in Peninsular Malaysia.