Catalog # Aliquot Size

P59-911B-05 3 x 5 nmol P59-911B-20 3 x 20 nmol P59-911B-50 3 x 50 nmol

PRKAR1B siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # P59-911B

Lot # Z2085-7

Specificity Formulation PRKAR1B siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human PRKAR1B expression. shipped at room temperature.

Product Description Reconstitution Protocol PRKAR1B siRNA is a pool of three individual synthetic Briefly centrifuge the tubes (maximum RCF 4,000g) to siRNA duplexes designed to knock-down human PRKAR1B collect lyophilized siRNA at the bottom of the tube. mRNA expression. Each siRNA is 19-25 bases in length. The Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_002735. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot PRKAR1 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or PRKAR1A P57-30H below -20oC. After resuspension, siRNA stock solutions ≥2 PRKAR1B Protein P59-30H µM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Scientific Background PRKAR1B is the type I-beta regulatory subunit of cyclic PRKAR1B siRNA Set I AMP-dependent (PKA) which is an siRNA duplexes targeted against three exon regions essential enzyme in the cAMP signaling pathway. PKA holoenzyme is composed of 2 regulatory and 2 catalytic Catalog # P59-911B subunits and dissociates from the regulatory subunits Lot # Z2085-7 upon binding of cAMP. PKA controls many biochemical Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol events in the cell including regulation of metabolism, ion Format Lyophilized powder transport, and gene transcription. PKA undergoes a Stability 1yr at -70oC from date of shipment dramatic conformational change upon complex Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is formation with the catalytic subunit (1). PRKAR1B subunits recommended that the lyophilized can dimerize through an N-terminal motif and this and resuspended siRNAs are stored at dimerization is necessary for binding to PKA anchoring or below -20oC. After resuspension, (AKAPs) and targeting of PKA to its site of action. siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles

without significant degradation. For References long-term storage, it is recommended 1. Gullingsrud J, et al. Dynamic binding of PKA that the siRNA is stored at -70oC. For regulatory subunit RI alpha.Structure, 2006 Jan. PMID most favorable performance, avoid repeated handling and multiple 16407073. freeze/thaw cycles. 2. Carlson, C R. et al: A kinase anchoring protein (AKAP) interaction and dimerization of the RIalpha and RIbeta regulatory subunits of protein kinase a in vivo by the yeast two hybrid system. J Mol Biol. 2003 Mar 28;327(3):609-18. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com

FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.