bioRxiv preprint doi: https://doi.org/10.1101/596734; this version posted April 2, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. VEGF and FGF signaling during head regeneration in hydra Anuprita Turwankar and Surendra Ghaskadbi* Developmental Biology Group, MACS-Agharkar Research Institute, Savitribai Phule Pune University, G.G. Agarkar road, Pune 411004, India *To whom correspondence should be addressed: Surendra Ghaskadbi Developmental Biology Group MACS-Agharkar Research Institute G.G. Agarkar Road, Pune-411 004, India Tel.: +91 20 2532506 Fax: +91 20 25651542 Email:
[email protected];
[email protected] Abbreviations: VEGF, vascular endothelial growth factor; FGF, fibroblast growth factor; FGFR-1, FGF receptor 1 and VEGFR-2, VEGF receptor 2; A, adenine; T, thymine; G, guanine; C, cytosine; bp, base pairs; cDNA, DNA complementary to RNA; DNA, deoxyribonucleic acid; RNA, ribonucleic acid; DMSO, dimethyl sulfoxide; NCBI, National Center for Biotechnology Information; BLAST, Basic Local Alignment Search Tool; MEGA, Molecular Evolutionary Genetic Analysis; PDB, Protein Data Bank; UniProt, Universal Protein Resource. bioRxiv preprint doi: https://doi.org/10.1101/596734; this version posted April 2, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract: Background: Vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) signaling pathways play important roles in the formation of the blood vascular system and nervous system across animal phyla. We have earlier reported VEGF and FGF from Hydra vulgaris Ind-Pune, a cnidarian with a defined body axis, an organized nervous system and a remarkable ability of regeneration.