Human Carbonic Anhydrase 12 (25-301, His-Tag) - Purified

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AR51944PU-S Human Carbonic anhydrase 12 (25-301, His-tag) - Purified

Alternate names: CA-XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase XII, HOM-RCC-3.1.3 tumor antigen Quantity: 50 µg Concentration: 0.5 mg/ml (determined by absorbance at 280nm) Background: CA12, also known as Carbonic anhydrase 12, is an enzyme that in humans is encoded by the CA12 gene. Carbonic anhydrases(CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Uniprot ID: O43570 NCBI: NP_001209 GeneID: 771 Species: Human Format: State: Liquid purified protein Purity: >90% by SDS - PAGE Buffer System: Phosphate buffered saline (pH7.4) containing 10% glycerol. Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method) Description: Recombinant human CA12, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. AA Sequence: APVNGSKWTY FGPDGENSWS KKYPSCGGLL QSPIDLHSDI LQYDASLTPL EFQGYNLSAN KQFLLTNNGH SVKLNLPSDM HIQGLQSRYS ATQLHLHWGN PNDPHGSEHT VSGQHFAAEL HIVHYNSDLY PDASTASNKS EGLAVLAVLI EMGSFNPSYD KIFSHLQHVK YKGQEAFVPG FNIEELLPER TAEYYRYRGS LTTPPCNPTV LWTVFRNPVQ ISQEQLLALE TALYCTHMDD PSPREMINNF RQVQKFDERL VYTSFSQVQV CTAAGLSHHH HHH Molecular weight: 31.94 kDa (283aa) Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: Tureci O. et al. (1998) Proc Natl Acad Sci U S A. 95(13):7608-13. Kyllonen MS. et al. (2003) J Histochem Cytochem. 51(9):1217-24.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20150127 1 / 2 AR51944PU-S: Human Carbonic anhydrase 12 (25-301, His-tag) - Purified

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For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MP/20150127 2 / 2