SPOP Antibody Cat. No.: 26-515
SPOP Antibody
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human SPOP.
TESTED APPLICATIONS: ELISA, WB
SPOP antibody can be used for detection of SPOP by ELISA at 1:1562500. SPOP antibody APPLICATIONS: can be used for detection of SPOP by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
POSITIVE CONTROL: 1) Cat. No. 1211 - HepG2 Cell Lysate
PREDICTED MOLECULAR 42 kDa WEIGHT:
Properties
PURIFICATION: Antibody is purified by peptide affinity chromatography method.
CLONALITY: Polyclonal
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
October 2, 2021 1 https://www.prosci-inc.com/spop-antibody-26-515.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.
CONCENTRATION: batch dependent
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPOP STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
OFFICIAL SYMBOL: SPOP
ALTERNATE NAMES: SPOP, TEF2, BTBD32
ACCESSION NO.: NP_001007227
PROTEIN GI NO.: 56117836
GENE ID: 8405
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
SPOP may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone- associated proteins. In mouse, the protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co- repressor complexes. This gene encodes a protein that may modulate the transcriptional BACKGROUND: repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.
REFERENCES: 1) Bunce, M.W., (2008) J. Biol. Chem. 283 (13), 8678-8686.
ANTIBODIES FOR RESEARCH USE ONLY.
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