SAN TA C RUZ BI OTEC HNOL OG Y, INC . Neurophysin I (D-11): sc-393907

BACKGROUND PRODUCT The nonapeptide Arginine (ARG-Vasopressin or AVP) Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% and Oxytocin are synthesized in the supraoptic and paraventricular nuclei of sodium azide and 0.1% gelatin. the together with their respective ”carrier” , the neuro - Blocking peptide available for competition studies, sc-393907 P, (100 µg physins. Vasopressin and are produced by separate populations of peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% stabilizer magnocellular neurons in both nuclei. Neurophysin I (NPI) and Neurophysin II ). (NPII) function as carrier proteins for Oxytocin and Vasopressin, respectively. Oxytocin is a pituitary which induces uterine contractions during APPLICATIONS childbirth and the ejection of milk from the mammary glands during nursing. Vasopressin is involved in the metabolism of water and electrolytes and has Neurophysin I (D-11) is recommended for detection of precursor and mature been identified as a vasoconstrictor. Both Neurophysin genes exist as three Neurophysin I of mouse and rat origin by Western Blotting (starting dilution exons, with each exon encoding a functional protein domain. Studies show 1:100, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100- that the identically conserved middle region (exon B) is involved in NP-NP 500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting homodimer formation as well as being the site for the Glycine 17 to Valine dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting point mutation responsible for familial diabetes insipidus. The genes encoding dilu tion 1:30, dilution range 1:30-1:3000). Neurophysin I and II map to human chromosome 20p13. Suitable for use as control antibody for Neurophysin I siRNA (m): sc-60090, Neurophysin I shRNA Plasmid (m): sc-60090-SH and Neurophysin I shRNA (m) REFERENCES Lentiviral Particles: sc-60090-V. 1. Brownstein, M.J., et al. 1980. Synthesis, transport, and release of posterior Molecular Weight of Neurophysin I: 10-19 kDa. pituitary hormones. Science 207: 373-378. Positive Controls: mouse extract: sc-364246 or rat pituitary 2. North, W.G., et al. 1980. Isolation and partial characterization of two human tissue extract. : their use in the development of specific radioimmunoassays. J. Clin. Endocrinol. Metab. 51: 884-891. RECOMMENDED SUPPORT REAGENTS 3. Ruppert, S., et al. 1984. Recent gene conversion involving bovine Vaso- To ensure optimal results, the following support reagents are recommended: pressin and Oxytocin precursor genes suggested by nucleotide sequence. 1) Western Blotting: use m-IgG κ BP-HRP: sc-516102 or m-IgG κ BP-HRP (Cruz Nature 308: 554-557. Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ 4. Doris, P.A. 1984. Vasopressin and central integrative processes. Molecular Weight Standards: sc-2035, UltraCruz ® Blocking Reagent: Neuro endocrinology 38: 75-85. sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopre- cipitation: use Protein L-Agarose: sc-2336 (0.5 ml agarose/2.0 ml). 3) Immu- 5. Abercrombie, D.M., et al. 1984. Cooperative interactions in Neurophysin- nofluorescence: use m-IgG κ BP-FITC: sc-516140 or m-IgG κ BP-PE: sc-516141 neuropeptide hormone complexes. Analytical affinity chromatography of (dilution range: 1:50-1:200) with UltraCruz ® Mounting Medium: sc-24941 or native and covalently-modified Neurophysins. Int. J. Pept. Protein Res. 24: UltraCruz ® Hard-set Mounting Medium: sc-359850. 218-232. 6. Gopal Rao, V.V., et al. 1992. The human gene for Oxytocin-Neurophysin I DATA (OXT) is physically mapped to chromosome 20p13 by in situ hybridization. Cytogenet. Cell Genet. 61: 271-273. AB 7. Olias, G., et al. 1996. Heterologous expression of human vasopressin- 35K–

neu rophysin precursors in a pituitary cell line: defective transport of a 22K– mutant protein from patients with familial diabetes insipidus. DNA Cell 19K– Biol. 15: 929-935. Neurophysin I

6K– CHROMOSOMAL LOCATION

Genetic locus: Oxt (mouse) mapping to 2 F1. Neurophysin I (D-11): sc-393907. Western blot Neurophysin I (D-11): sc-393907. Immunofluorescence analysis of Neurophysin I expression in mouse staining of methanol-fixed NIH/3T3 cells showing SOURCE pituitary gland (A) and rat pituitary (B) tissue extracts. membrane and cytoplasmic localization. Neurophysin I (D-11) is a mouse monoclonal antibody specific for an epitope STORAGE mapping between amino acids 101-127 near the C-terminus of Neurophysin I of mouse origin. Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. RESEARCH USE For research use only, not for use in diagnostic procedures.

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