Anti-PDCD1LG2 Monoclonal Antibody, Clone UZ36 [Pacific Blue™] (CABT-51712RM) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use
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Anti-PDCD1LG2 monoclonal antibody, clone UZ36 [Pacific Blue™] (CABT-51712RM) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Antigen Description CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production. Specificity Rat anti Mouse CD273 antibody, clone UZ36 recognises mouse CD273, a 42kD member of the B7 family, otherwise known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4. Antibodies produced by clone UZ36 are reported to block the binding of CD273 (B7-DC) to it's receptor. Removal of Sodium Azide is recommended prior to use in functional assays. Immunogen B7-DC transfected RAW264.7 cells. Isotype IgG2a Source/Host Rat Species Reactivity Mouse Clone UZ36 Conjugate Pacific Blue™ Applications Flow Cytometry Preparation Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Procedure Conjugated Antibodies Format Purified IgG conjugated to Pacific Blue? - liquid. Concentration IgG concentration 0.05 mg/ml. Size 25 Tests/0.25ml Buffer Phosphate buffered saline. Preservative 0.09% Sodium Azide Storage Store at +4°C or at -20°C if preferred. 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. GENE INFORMATION Gene Name Pdcd1lg2 programmed cell death 1 ligand 2 [ Mus musculus (house mouse) ] Official Symbol Pdcd1lg2 Synonyms Pdcd1lg2; programmed cell death 1 ligand 2; Btdc; B7-DC; PD-L2; F730015O22Rik; programmed cell death 1 ligand 2; PD-1 ligand 2; PDCD1 ligand 2; butyrophilin B7-DC; butyrophilin-like protein; programmed death ligand 2 Entrez Gene ID 58205 Protein Refseq NP_067371 UniProt ID Q3U304 Chromosome Location 19; 19 C2-C3 Pathway Adaptive Immune System; Cell adhesion molecules (CAMs); Costimulation by the CD28 family; Immune System; PD-1 signaling Function protein binding References 1. Matsumoto, K. et al. (2004) B7-DC regulates asthmatic response by an IFN-gamma- dependent mechanism. J. Immunol.172: 2530 – 2541. 2. Yamazaki, T. et al. (2002) Expression of programmed death 1 ligands by murine T cells and APC. J. Immunol.169: 5538 – 5545. 3. Kanai, T. et al. (2003) Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J. Immunol.171: 4156 – 4163. 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.