Anti-BORIS Antibody [4A7] (ARG10959)

Product datasheet [email protected]

ARG10959 Package: 100 μg anti-BORIS antibody [4A7] Store at: -20°C

Summary

Product Description Mouse Monoclonal antibody [4A7] recognizes BORIS

Tested Reactivity Hu

Tested Application ChIP, ELISA, ICC/IF, IP, WB

Host Mouse

Clonality Monoclonal

Clone 4A7

Isotype IgM, kappa

Target Name BORIS

Antigen Species Human

Immunogen Synthetic peptide around aa. 2-257 (N-terminus) of Human BORIS.

Conjugation Un-conjugated

Alternate Names BORIS; Brother of the regulator of imprinted sites; Cancer/testis antigen 27; CCCTC-binding factor; CTCF- like protein; Transcriptional repressor CTCFL; Zinc finger protein CTCF-T; dJ579F20.2; CTCF-T; CT27; CTCF paralog; HMGB1L1

Application Instructions

Application table Application Dilution

ChIP Assay-dependent

ELISA Assay-dependent

ICC/IF Assay-dependent

IP Assay-dependent

WB Assay-dependent

Application Note ChIP and IP: Protein L beads should be used for ChIP and IP because this antibody is IgM kappa. ICC/IF: Staining Pattern: May be nuclear, cytoplasmic or both (depending on cell type) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Calculated Mw 76 kDa

Properties

Form Liquid

Purification Purification with Protein A.

Buffer PBS and 0.02% Sodium azide.

www.arigobio.com 1/2 Preservative 0.02% Sodium azide

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CTCFL

Gene Full Name CCCTC-binding factor (zinc finger protein)-like

Background CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X- chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Function Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters. [UniProt]

Images

ARG10959 anti-BORIS antibody [4A7] ChIP image

ChIP: Optimization of ChIP with ARG10959 anti-BORIS antibody [4A7] in LNCaP cells using known CTCF target sites (CTS). Agarose gel analyses of PCR products from ChIP assays using ARG10959 anti- BORIS antibody [4A7] immobilized on Protein L agarose beads. (A) PCR products were tested using primers for the known CTS within the human PIM-1 gene. Both BORIS and CTCF showed DNA enrichment at the PIM-1 site. (B) PCR performed for another characterized CTS (H19 ICR). A stronger binding at the H19 site in CTCF ChIP DNA was observed as compared to BORIS ChIP DNA.

No-antibody reactions for both targets do not show any PCR product. In all ChIP experiments, 1% of sonicated cross-linked cell lysate was used as positive control (Input).