Efficient Professional and Antibody Platforms USP22 Antibody Basic information: Catalog No.: UMA60855 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: Protein A affinity purified Useful Information: WB:1:100-1:1000 IP:1-2ug per 100-500ug of total protein (1ml of cell lysate) Applications: IF:1:50-1:500 ELISA:1:30-1:3000 Reactivity: Human Specificity: This antibody recognizes USP22 protein. Amino acids 130-176 mapping within an internal region of USP22 of human Immunogen: origin. The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progres- sion. USP22 (ubiquitin specific peptidase 22), also known as USP3L, is a 525 Description: amino acid protein that contains one UBP-type zinc finger and functions to catalyze the conversion of a ubiquitin C-terminal thioester to free ubiquitin and thiol, a reaction that may influence several cellular processes. Via its catalytic activity, USP22 is thought to play an important role in cell cycle progression and may also serve as a cancer stem cell marker. The en- coding USP22 maps to human 17, which comprises over 2.5% of the and encodes over 1,200 . Uniprot: Q9UPT9 BiowMW: 60 kDa Buffer: 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data:

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Western blot analysis of USP22 expression in HeLa (A) and Jurkat (B) whole cell lysates.

Western blot analysis of USP22 expression in Jurkat (A), Caco-2 (B) and F9 (C) whole cell ly- sates.

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]