Research Collection

Journal Article

The for the β Subunit of the Follicle-Stimulating Maps to Bovine 15

Author(s): Fries, R.

Publication Date: 1989

Permanent Link: https://doi.org/10.3929/ethz-b-000422815

Originally published in: Journal of heredity 80(5), http://doi.org/10.1093/oxfordjournals.jhered.a110883

Rights / License: In Copyright - Non-Commercial Use Permitted

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ETH Library Table 2. Result* of Inoculating F, plants of Individual F, plants from the crosses of 0X686 (RsvT RaoT) x L78-379 (Rtcl RMCT) and 0X686 x OX670 QRMD2 RICZ) with the Gl or G4 strain of soybean mosaic The Gene for the 0 Subunit virus of the Follicle-Stimulating 0X686 x L78-379 0X686 x OX670* Hormone Maps to Bovine

F3 Expected Observed Expected Observed Expected Chromosome 15 classification" F, genotype ratio no. no. no. no. R. Fries AllR RsvRso* 4 19 20.25 15 14.25 Rand STN Rsvnv KsvfRsv? 2 11 10.12 8 7.12 R, STN, and M Rsvnv RsvTrsv? 4 20 20.25 16 14.25 Using a 1,500-bp cDNA specific for the /3 Rand M Rsvnv nv?nv? 2 9 10.12 7 7.12 All STN rsvnv Rsv?Rsv7 1 5 5.06 2 3.56 subunit of bovine follicle-stimulating hormone STN and M nonv RSUTTSV? 2 12 10.12 6 7.12 as a probe for in situ hybridization, I assigned All M rsvnv nv?nv? 1 5 5.06 3 3.56 the corresponding locus, FSHB, to bovine X1 063 1.31 P .99 95-.98 chromosome 15q24-qter. Previous assign- ments of the loci for /3-globin (HBB) and para- 'R- resistant (no mosaic or necrosis); STN — stem-tip necrosis; M •= mosaic (susceptible). hormone (PTH) to chromosome 15, "0X686 STN (29 plants); OX670 R (10 plants). along with my assignment, indicate the evo- ' Rsvl/nvl for first cross; Rsv2/nv2 for second cross. lutionarily conserved synteny of these loci on the short arm of human and on bovine chromosome 15. The analysis of cur which result in a stem-tip necrosis re- segregation, indicating that Rsv3 is not the distribution of the autoradiographic silver action and plant death. The epistatic effect linked with Rsvl or Rsv2. grains suggests that FSHB is more distalty of Rsvl and Rsv2 on Rso3 we observed In the F2 generation of the crosses of located than are HBB and PTH, contrary to indicates that the use of two Rsv to 0X686 with Harosoy, there were 80 Rso3_ the order of these loci on the short arm of provide mosaic resistance in a cultivar Rmd_, 27 Rsv3_ rmdrmd, 27 rsv3rsv3 human chromosome 11. Rmd , and 6 rsv3rsu3 rmdrmdplants. This should reduce the risk of this happening. indicates independent segregation for the From the Agriculture Canada Research Station, Har- Attempts have been made to establish sys- two gene pairs that entered the cross in row, Ontario. Address reprint requests to Dr. Buzzell, tematically the gene maps of domestic the coupling phase. Agriculture Canada, Research Station, Harrow, Ontario NOR 1G0, Canada. species of animals by identifying evolu- tionarily conserved segments of chromo- Discussion somes.12 We have shown the conserved 3 References linkage of the loci for parathyroid hor- Cho et ah suggested that necrosis reac- mone {PTH) and /9-globin (//SB) in cattle tion and mosaic resistance in the cultivar 1 Buzzell RI, and Haas JH. Inheritance of adult plant resistance to powdery mildew in soybeans. Can J Ge- and have assigned the linkage group to 'Kwangkyo' (PI 406710) might be effects net Cytol 1978; 20:151-153. bovine chromosome \5.* PTH and HBB of the same gene for SMV resistance. The 2. Buzzell RI, and Tu JC. Inheritance of soybean resis- map to the short arm of chromosome 11 dominant Kwangkyo gene could be an Rsv tance to soybean mosaic virus. J Hered 1984; 75:82. (1 lp) in humans and to chromosome 7 in gene similar to the Rsu3 gene. Kwangkyo 3. Cho EK, Chung BJ, and Lee SH. Studies on identi- mice, respectively. From a comparative produces a necrotic reaction with strains fication and classification of soybean virus disease in Korea. II. Etiology of a necrotic disease of Gtycine max. point of view, human lip can be divided G5, G6, and G7 and a symptomless reac- Plant Dis Rept 1977; 61:313-317. tion with strains Gl, G2, G3, and G4/ into at least two segments: a distal region 4. Cho EK, and Goodman RM. Strains of soybean mo- containing PTH and HBB, corresponding whereas strains Gl through G7 produce saic virus: classification based on virulence in resistant some STN in 0X686, with Gl and G4 show- soybean cultivars. Phytopathology 1979; 69:467-470. to a part of murine chromosome 7, and a proximal region containing the loci for cat- ing a more severe reaction (J. C. Tu, un- 5. Cho EK, and Goodman RM. Evaluation of resistance published results). In soybeans to soybean mosaic virus strains. Crop Sci alase {CAT) and the /S-polypeptide of the 1982; 22.1133-1136. follicle-stimulating hormone {FSHB), cor- Local lesions often occur on the primary 6. KJihl RAS, and Hartwig EE. Inheritance of reaction responding to a part of murine chromo- leaves of plants that develop stem-tip ne- to soybean mosaic virus in soybeans. Crop Sci 1979; some 2.10 Genes from both regions map to crosis when they are inoculated with ne- 19:372-375. 8 4 chromosome Dl of the cat. Because the crosis-causing strains of SMV (J. C. Tu, 7. Kwon SH, and Oh JH. Resistance to a necrotic strain unpublished results). This supports the of soybean mosaic virus In soybean. Crop Sci 1980; 20: bovine gene for the /S-polypeptide of FSHB concept that STN is a hypersensitive (re- 403-404. has been cloned, I attempted to determine 8. Urn SM. Resistance to soybean mosaic virus in soy- its chromosomal position in cattle by in sistant) reaction. Thus, if stem-tip necro- beans. Phytopathology 1985; 75:199-201. sis is the result of an incompatible reaction situ hybridization to delineate the extent 9. Tu JC, and Buzzell RI. Stem-tip necrosis: a hyper- of the conservation of human 11 p on cattle between the soybean and an SMV strain, sensitive, temperature dependent, dominant gene re- it should not be classified as a "suscepti- action of soybean to infection by soybean mosaic virus. chromosome 15. I present evidence that ble" reaction. Considering STN as a resis- Can J Plant Sci 1987; 67-661-665. FSHB also maps to bovine chromosome tant reaction affects the genetic interpre- 15. tation. For example, Lim8 and Kwon and Oh7 considered STN to be a susceptible Materials and Methods 6 reaction, whereas Kiihl and Hartwig rated I obtained chromosome spreads from fi- STN plants as being resistant. broblast cell lines by the mitotic shake-off As noted by Cho and Goodman,'1 the use technique. The fibroblast cell lines had of Rsu genes to give resistance to mosaic been established from fetal bovine tissue. carries the risk that SMV strains may oc- Metaphase spreads were QFQ-banded and

Brief Communications 401 9 3 5 3 1 3 6 touching achromosome,10.7 % wereas- grain (0.55/im).Thebandidiogra m shown tion ofsilvergrainsalongtheGiemsa- tion i102Giemsa-stainedmetaphase 402 The JournalofHererJHy : 198930(5} Reading standard.Of606silve r grains in Figure1waspreparedaccordin g tothe scaled totheaveragdiamete r ofasilve grains wereassignedtoonf324unit wereidentified,andth stained chromosomeswaestimated,the before insituhybridizatiotoallowchro- spreads. AllspreadswereQFQ-banded radiographic silvergrainsaftehybridiza- Figure 1showsthdistributionofauto- Results 20 days. mosome identification.Threlativposi- radiography ofslideswadonefor14t The concentrationofthprobihy- bridization wasdoneaccordingtoth Figure 1.Histogramshowingthdistributionofautoradiographicsilvergrainsfro102metaphasspreadafteisituhybridizatiowithaFSHB-specificsequence photographed priortoinsituhybridiza- bridization solutiowas20ng/ml.Auto- modifications adescribedbyFrieetal. method ofHarperanSaunders,with salmon spermDNA(10ng).Isituhy- cipitation ithepresencofalkali-sheared column andwasrecoverebyethanolpre- side triphosphateswithaSephadexG-50 separated fromunincorporatenucleo- tritiated nucleotides.ThelabeleDNAwas tivity of2x108dpm/MgDNA,usinthree cDNA insertexcisedwithHindlllanEcdR\ tion, asdescribedelsewhere.Theprob random primingmethodtoaspecificac- from arecombinantpGEM3plasmid.The for theFSHBlocuswaa1,500-bpbovin "o FSHB-specific sequencewaslabeledbyth mber o> •ains 20 30 20 20 10 10 10 1( IIIH2IIHIIIHIIiai3BHill4•••Bil5HB6IlllliWtl71IBI9H8LUILUIJ 9(IIIIIIIII 1ocmnnKJIIJJL12cmiu13an14cmscum]6

References 1. Feinberg AP, and Vogelstein B. A technique for ra- diolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1983; 132:6-13. 2. Feinberg AP, and Vogelstein B A technique for ra- diolabeling DNA restriction endonuclease fragments to high specific activity (addendum). Anal Biochem 1984, 137:266-267. 3. Fries R, Hedlger R, and Stranzinger G. Tentative chromosomal localization of the bovine major histo- compatibility complex by in situ hybridization Anlm Genet 1986; 17:287-294. 4. Fries R, Hediger R, and Stranzinger G. The loci for and beta-globln are closely linked and map to chromosome 15 in cattle Genomics 1988, 3.302-307 5. Harper ME, and Saunders GF. Localization of single copy DNA sequences on G-banded human chromo- Heterozyqote somes by in situ hybridization. Chromosome 1981; 83: 431^139 6 Maurer RA, and Beck A. Isolation and nucleotide sequence analysis of a cloned cDNA encoding the beta- subunit of bovine follicle-stimulating hormone. DNA 1986; 5:363-369. 7. Nadeau JH, and Taylor BA. Lengths of chromosomal segments conserved since divergence of man and mouse. Proc Nat] Acad Sci USA 1984; 81:814-818. 8. O'Brien SJ, Hasklns ME, Winkler CA, Nash WG, and Patterson DF. Chromosomal mapping of beta-globln and albino loci in the domestic cat. J Hered 1986; 77 374-378 9. Proceedings of the First International Conference for the Standardisation of Banded Karyotypes of Do- mestic Animals (Ford CE, Pollok DL, and Gustavsson I, eds). Hereditas 1980; 92:145-162. 10. Searle AG, Peters J, Lyon MF, Evans EP, Edwards JH, and Buckle VJ. Chromosome maps of man and mouse, III. Genomics 1987; 1:3-18. 11. Womack J, and Moll YD. Gene map of the cow: conservation of linkage with mouse and man. J Hered 1986; 77:2-7. 12. Womack JE. Genetic engineering in agriculture: an- Figure 1. Diagram showing variation of black scaling on the hind tarsomere V of A aegypti. (a) W — wild-type, imal genetics and development. Trends Genet 1987; 3 all-white scaling on tarsomere V. (b) about six black scales at the tip, seen in the F, progeny when wild-type 65-68 and COM were crossed, (c) COM - half-black tarsomere V. (d) bit - all-black tarsomeres.

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