MHC Class II Proteins Mediate Cross-Species Entry of Bat Influenza Viruses Umut Karakus1,17, Thiprampai Thamamongood2,3,4,5,17, Kevin Ciminski2,3, Wei Ran2,3, Sira C
LETTER https://doi.org/10.1038/s41586-019-0955-3 MHC class II proteins mediate cross-species entry of bat influenza viruses Umut Karakus1,17, Thiprampai Thamamongood2,3,4,5,17, Kevin Ciminski2,3, Wei Ran2,3, Sira C. Günther1, Marie O. Pohl1, Davide Eletto1, Csaba Jeney6, Donata Hoffmann7, Sven Reiche8, Jan Schinköthe8, Reiner Ulrich8, Julius Wiener9, Michael G. B. Hayes10, Max W. Chang10, Annika Hunziker1, Emilio Yángüez1, Teresa Aydillo11,12, Florian Krammer11, Josua Oderbolz13, Matthias Meier9, Annette Oxenius13, Anne Halenius2,3, Gert Zimmer14,15, Christopher Benner10, Benjamin G. Hale1, Adolfo García-Sastre11,12,16, Martin Beer7, Martin Schwemmle2,3,18* & Silke Stertz1,18* Zoonotic influenza A viruses of avian origin can cause severe disease To identify receptor candidates for bat IAV, we performed tran- in individuals, or even global pandemics, and thus pose a threat to scriptional profiling on three cell lines that are susceptible to bat IAV human populations. Waterfowl and shorebirds are believed to be (Madin–Darby canine kidney II (MDCKII) clone no. 1, and human the reservoir for all influenza A viruses, but this has recently been glioblastoma (U-87MG) and lung cancer (Calu-3) cell lines), and on challenged by the identification of novel influenza A viruses in three cells lines that are not susceptible (MDCKII clone no. 2, and bats1,2. The major bat influenza A virus envelope glycoprotein, human adenocarcinomic alveolar basal epithelial (A549) and human haemagglutinin, does not bind the canonical influenza A virus glioblastoma (U-118MG) cell lines). The susceptibility of each cell receptor, sialic acid or any other glycan1,3,4, despite its high sequence line was characterized by two different H18- or H18N11-pseudotyped and structural homology with conventional haemagglutinins.
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