US 2015O141245A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2015/0141245 A1 Wachendorff-Neumann et al. (43) Pub. Date: May 21, 2015

(54) COMPOSITIONS COMPRISINGA (30) Foreign Application Priority Data BOLOGICAL CONTROL AGENT AND A FUNGCIDE FROM THE GROUP May 30, 2012 (EP) ...... 120041603

CONSISTING OF INHIBITORS OF THE Dec. 19, 2012 (EP) ------12 197951 .2 RESPRATORY CHAIN AT COMPLEXOR II Publication Classification

(71) Applicant: BAYER CROPSCIENCE AG, (51) Int. Cl. Monheim (DE) AOIN 63/00 (2006.01) (72) Inventors: Ulrike Wachendorff-Neumann, 39ty 7.2% :08: Neuwied (DE); Wolfram Andersch, AOIN37/24 (2006.015 Bergisch Gladbach (DE); Klaus Stenzel, (52) U.S. Cl t (DE); Bernd Springer, CPC ...... A0IN 63/00 (2013.01); A0IN37/24 Koeln (DE) (2013.01); A0IN 43/56 (2013.01); A0IN 43/40 (21) Appl. No.: 14/404,026 (2013.01) (22) PCT Filed: May 29, 2013 (57) ABSTRACT The ppresent invention relates to a compositionp comprisingpr1S1ng at (86). PCT No.: PCT/EP2013/061O12 least one biological control agent. Furthermore, the present S371 (c)(1) invention relates to the use of this composition as well as a

(2) Date: Nov.e 26,a? V.9 2014 method for reducing overall damage of plants and plant parts. US 2015/O 141245 A1 May 21, 2015

COMPOSITIONS COMPRISINGA the activity spectrum of the biological control agents and BIOLOGICAL CONTROL AGENT ANDA fungicides, and thereby to provide a composition which, pref FUNGCIDE FROM THE GROUP erably at a reduced total amount of active compounds applied, CONSISTING OF INHIBITORS OF THE has improved activity against insects, mites, nematodes and/ RESPRATORY CHAIN AT COMPLEXOR II orphytopathogens. In particular, it was a further object of the 0001. The present invention relates to a composition com present invention to provide a composition which, when prising at least one biological control agent selected from applied to a crop, results in a decreased amount of residues in specific microorganisms and/or a mutant of these strains hav the crop, thereby reducing the risk of resistance formation and ing all the identifying characteristics of the respective strain, nevertheless provides efficient disease control. and/or a metabolite produced by the respective strain that 0009. Accordingly, it was found that these objects at least exhibits activity against insects, mites, nematodes and/or partly are solved by the compositions according to the inven phytopathogens and at least one fungicide (I) selected from tion as defined in the following. The composition according to the group consisting of inhibitors of the respiratory chain at the present invention preferably fulfills the above-described complex I or II in a synergistically effective amount. Further needs. It has been Surprisingly discovered that the application more, the present invention relates to the use of this compo of the composition according to the present invention in a sition as well as a method for reducing overall damage of simultaneous or sequential way to plants, plant parts, har plants and plant parts. vested fruits, vegetables and/or plant's locus of growth pref 0002 Synthetic insecticides or fungicides often are non erably allows better control of insects, mites, nematodes and/ specific and therefore can act on organisms other than the or phytopathogens than it is possible with the strains, their target ones, including other naturally occurring beneficial mutants and/or their metabolites produced by the strains on organisms. Because of their chemical nature, they may be the one hand and with the individual fungicides on the other also toxic and non-biodegradable. Consumers worldwide are hand, alone (synergistic mixtures). By applying the biologi increasingly conscious of the potential environmental and cal control agent and the specified fungicide according to the health problems associated with the residuals of chemicals, invention the activity against insects, mites, nematodes and/ particularly in food products. This has resulted in growing or phytopathogens is preferably increased in a Superadditive consumer pressure to reduce the use or at least the quantity of manner. Preferably, the application of the composition chemical (i.e. synthetic) pesticides. Thus, there is a need to according to the invention induces an increase in the activity manage food chain requirements while still allowing effec of phytopathogens in a Superadditive manner. tive pest control. 0003. A further problem arising with the use of synthetic 0010. As a consequence, the composition according to the insecticides or fungicides is that the repeated and exclusive present invention preferably allows a reduced total amount of application of an insecticide or fungicides often leads to active compounds to be used and thus the crops which have selection of resistant microorganisms. Normally, Such strains been treated by this composition preferably show a decreased are also cross-resistant against other active ingredients having amount of residues in the crop. Accordingly, the risk of resis the same mode of action. An effective control of the patho tance formation of harmful microorganisms is decreased. gens with said active compounds is then not possible any 0011. The present invention is directed to a composition longer. However, active ingredients having new mechanisms comprising at least one biological control agent selected from of action are difficult and expensive to develop. the group consisting of Bacillus chitinosporus AQ746 0004. The risk of resistance development in pathogen (NRRL Accession No. B-21618), Bacillus mycoides AQ726 populations as well as environmental and human health con (NRRL Accession No. B-21664), Bacillus pumilus (NRRL cerns have fostered interest in identifying alternatives to Syn Accession No. B-30087), Bacillus pumilus AQ717 (NRRL thetic insecticides and fungicides for managing plant dis Accession No. B-21662), Bacillus sp. AQ175 (ATCC Acces CaSCS. sion No. 55608), Bacillus sp. AQ177 (ATCC Accession No. 0005. The use of biological control agents (BCAs) is one 55609), Bacillus sp. AQ178 (ATCC Accession No. 53522), alternative. However, the effectiveness of most BCAS is notat Bacillus subtilis AQ743 (NRRL Accession No. B-21665), the same level as for conventional insecticides and fungi Bacillus subtilis AQ713 (NRRL Accession No. B-21661), cides, especially in case of severe infection pressure. Conse Bacillus subtilis AQ153 (ATCC Accession No. 55614), quently, known biological control agents, their mutants and Bacillus thuringiensis BD#32 (NRRL Accession No. metabolites produced by them are, in particular in low appli B-21530), Bacillus thuringiensis AQ52 (NRRL Accession cation rates, not entirely satisfactory. No. B-21619), Muscodor albus 620 (NRRL Accession No. 0006 Thus, there is a constant need for developing new, 30547), Muscodor roseus A3-5 (NRRL Accession No. alternative plant protection agents which in some areas at 30548), Rhodococcus globerulus AQ719 (NRRL Accession least help to fulfill the above-mentioned requirements. No. B-21663), Streptomyces galbus (NRRL Accession No. 0007 Example 13 of WO98/50422 discloses a synergistic 30232), Streptomyces sp. (NRRL Accession No. B-301.45), effect of a mixture comprising Bacillus subtilis AQ713 Bacillus thuringiensis subspec. Kurstaki BMP 123, Bacillus (NRRL Accession No. B-21661) and azoxystrobin. However, subtilis AQ30002 (NRRL Accession No. B-50421), and due to the nature of synergism it is not possible to predict the Bacillus subtilis AQ30004 (NRRL Accession No. B-50455) effect of other biological control agents in combination with and/or a mutant of these strains having all the identifying other fungicide based on this specific example. characteristics of the respective strain, and/or a metabolite 0008. In view of this, it was in particular an object of the produced by the respective Strain that exhibits activity against present invention to provide compositions which exhibit insects, mites, nematodes and/or phytopathogens and at least activity against insects, mites, nematodes and/or phytopatho one fungicide (I) selected from the group consisting of inhibi gens. Moreover, it was a further particular object of the tors of the respiratory chain at complex I or II in a synergis present invention, to reduce the application rates and broaden tically effective amount. US 2015/O 141245 A1 May 21, 2015

0012. Furthermore, the present invention relates to a kit of otide residues. The hydrogen bonding may occur by Watson parts comprising at least one of the specific biological control Crick base pairing, Hoogstein binding, or in any other agents and the at least one fungicide (I). The present invention sequence-specific manner. The complex may comprise two is further directed to the use of said composition as fungicide Strands forming a duplex structure, three or more Strands and/or insecticide. Moreover, it is directed to the use of said forming a multi-stranded complex, a single self-hybridizing composition for reducing overall damage of plants and plant Strand, or any combination of these. Hybridization reactions parts as well as losses in harvested fruits or vegetables caused can be performed under conditions of different “stringency’. by insects, mites, nematodes and/or phytopathogens. In general, a low stringency hybridization reaction is carried 0013 Moreover, the present invention provides a method out at about 40°C. in 10xSSC or a solution of equivalentionic for reducing overall damage of plants and plant parts as well strength/temperature. A moderate stringency hybridization is as losses in harvested fruits or vegetables caused by insects, typically performed at about 50° C. in 6xSSC, and a high mites, nematodes and/or phytopathogens. stringency hybridization reaction is generally performed at about 60° C. in 1XSSC. Biological Control Agents 0023. A variant of the indicated NRRL or ATCC Acces 0014. In general “pesticidal' means the ability of a sub sion Number may also be defined as a strain having agenomic stance to increase mortality or inhibit the growth rate of plant sequence that is greater than 85%, more preferably greater pests. The term is used herein, to describe the property of a than 90% or more preferably greater than 95% sequence Substance to exhibit activity against insects, mites, nematodes identity to the genome of the indicated NRRL or ATCC and/or phytopathogens. In the sense of the present invention Accession Number. A polynucleotide or polynucleotide the term "pests’ include insects, mites, nematodes and/or region (or a polypeptide or polypeptide region) has a certain phytopathogens. percentage (for example, 80%, 85%, 90%, or 95%) of 0015. As used herein, “biological control is defined as "sequence identity to another sequence means that, when control of a pathogen and/or insect and/or an acarid and/or a aligned, that percentage of bases (or amino acids) are the nematode by the use of a second organism. Known mecha same in comparing the two sequences. This alignment and the nisms of biological control include enteric bacteria that con percent homology or sequence identity can be determined trol root rot by out-competing fungi for space on the Surface using software programs known in the art, for example, those of the root. Bacterial toxins, such as antibiotics, have been described in Current Protocols in Molecular Biology (F. M. used to control pathogens. The toxin can be isolated and Ausubel et al., eds., 1987) Supplement 30, section 7.7. 18. applied directly to the plant or the bacterial species may be Table 7.7.1. administered so it produces the toxin in situ. (0024 NRRL is the abbreviation for the Agricultural 0016 “Insecticides” as well as the term “insecticidal' ResearchService Culture Collection, an international deposi refers to the ability of a substance to increase mortality or tary authority for the purposes of deposing microorganism inhibit growth rate of insects. As used herein, the term strains under the Budapest treaty on the international recog “insects” includes all organisms in the class “Insecta'. The nition of the deposit of microorganisms for the purposes of term “pre-adult' insects refers to any form of an organism patent procedure, having the address National Center for prior to the adult stage, including, for example, eggs, larvae, Agricultural Utilization Research, Agricultural Research ser and nymphs. vice, U.S. Department of Agriculture, 1815 North university 0017. “Nematicides” and “nematicidal refers to the abil Street, Peroira, Ill. 61604 USA. ity of a substance to increase mortality or inhibit the growth (0025 ATCC is the abbreviation for the American Type rate of nematodes. In general, the term “nematode' comprises Culture Collection, an international depositary authority for eggs, larvae, juvenile and mature forms of said organism. the purposes of deposing microorganism Strains under the 0018 “Acaricide” and “acaricidal” refers to the ability of a Budapest treaty on the international recognition of the deposit Substance to increase mortality or inhibit growth rate of ecto of microorganisms for the purposes of patent procedure, hav parasites belonging to the class Arachnida, Sub-class Acari. ing the address ATCC Patent Depository, 10801 University 0019. The term “metabolite' refers to any compound, sub Blvd., Manassas, Va. 10110 USA. stance or byproduct of a fermentation of a microorganism that 0026. The biological control agents used in the present has pesticidal activity. invention are known in the art as follows: 0020. The term “mutant” refers to a variant of the parental Bacillus chitinosporus AQ746 (NRRL Accession No. strain as well as methods for obtaining a mutant or variant in B-21618) (in the following sometimes referred to as B1) is which the pesticidal activity is greater than that expressed by known from WO 98/2 1966 A2. It is specifically active against the parental strain. The “parent strain' is defined herein as the nematodes and insects and produces non-exotoxin, non-pro original Strain before mutagenesis. To obtain such mutants teinaceous, active metabolites in its Supernatant. Those the parental strain may be treated with a chemical Such as metabolites are active against nematodes and cockroaches, N-methyl-N'-nitro-N-nitrosoguanidine, ethylmethane but inactive against flies, corn rootworm or beet armyworm. Sulfone, or by irradiation using gamma, X-ray, or UV-irradia (0027 Bacillus mycoides AQ726 (NRRL Accession No. tion, or by other means well known to those skilled in the art. B-21664) (in the following sometimes referred to as B2) and 0021. A “variant is a strain having all the identifying its water-soluble metabolites kill or stunt insects such as corn characteristics of the NRRL or ATCC Accession Numbers as rootworm larvae and aphids (WO 99/09820 A1). indicated in this text and can be identified as having a genome (0028. As described in WO 00/58442 A1 Bacillus pumilus that hybridizes under conditions of high Stringency to the QST2808 (NRRL Accession No. B-30087) (in the following genome of the NRRL or ATCC Accession Numbers. sometimes referred to as B3) is able to inhibit abroad range of 0022 "Hybridization” refers to a reaction in which one or fungal plant diseases in vivo. Moreover, the combination of more polynucleotides react to form a complex that is stabi this strain with Bacillus thuringiensis enhances the insecti lized via hydrogen bonding between the bases of the nucle cidal activity of the latter. Commercially available formula US 2015/O 141245 A1 May 21, 2015

tions of this strain are sold under the tradenames SONATAR) on specific plant pathogens such as Alternaria, Phytophthora, and BALLADR Plus from AgraCest, Inc. USA. Botrytis, Rhizoctoizia and Sclerotinia. 0029. Bacillus pumilus AQ717 (NRRL Accession 0040 Commercially available formulation of Bacillus B-21662) (in the following sometimes referred to as B4) is thuringiensis subspec. Kurstaki BMP 123 (in the following known from WO99/10477 A1. It produces a metabolite that sometimes referred to as B18) are available under the trade exhibits pesticidal activity against corn rootworms, nema name BARITONER) from AgraGuest, Inc. USA. It is exhibits todes and beet armyworms. insecticidal activity and is effective on lepidopterous insects, 0030. The bacterial strains Bacillus sp. AQ175 (ATCC including loopers, armyworms and moths. BARITONE(R) is Accession No. 55608) (in the following sometimes referred distributed subject to EPA Reg. No. 62637-5-69592. to as B5), Bacillus sp. AQ 177 (ATCC Accession No. 55609) 0041. The strains Bacillus subtilis AQ30002 (also known (in the following sometimes referred to as B6) and Bacillus as QST30002) (NRRL Accession No. B-50421, deposited on sp. AQ178 (ATCC Accession No. 53522) (in the following Oct. 5, 2010) (in the following sometimes referred to as B19) sometimes referred to as B7) described in WO 98/21967 A1 and Bacillus subtilis AQ30004 (also known as QST30004) are effective in treating and protecting plants from above (NRRL Accession No. B-50455, deposited on Oct. 5, 2010) ground fungal and bacterial infections. (in the following sometimes referred to as B20) are known 0031. The metabolite-producing strain Bacillus subtilis from WO 2012/087980 A1, which is incorporated herein by AQ743 (NRRL Accession No. B-21665) (in the following reference. As described therein, these BCAS exhibit a broad sometimes referred to as B8) kills or stunts corn rootworm fungicidal and bactericidal activity. B19 and B20 have a larvae, beet armyworm larvae, fly adults and nematodes (cf. mutation in the Swra gene that results in impaired Swarming WO 99/09819). ability and enhanced plant health promotion compared to a 0032 Bacillus subtilis AQ713 (Accession No. B-21661), strain containing a wildtype SWr A gene. The mutation causes also named Bacillus subtilis QST713, (in the following some these BCAS to form a more robust biofilm than the wildtype times referred to as B9) exhibits broad fungicidal and bacte strain, thereby enhancing its fungicidal and bactericidal activ ricidal activity and also exhibits corn rootworm activity (WO ity. 98/50422 A1). Commercially available formulation of this 0042. In a preferred embodiment the composition of the strain are available under the tradenames SERENADER) present invention is characterized in that the biological con Max, SERENADER) Soil, SERENADER) Aso, SER trol agent is selected from the group consisting of Bacillus ENADER CPB and RHAPSODYR from Agra(Quest, Inc. pumilus (NRRL Accession No. B-30087) and Bacillus subti USA. lis AQ713 (NRRL Accession No. B-21661) and/or a mutant 0033 Bacillus subtilis AQ153 (ATCC Accession No. of these stains having all the identifying characteristics of the 55614) (in the following sometimes referred to as B10) as respective strain, and/or a metabolite produced by the respec described in WO 98/21964 A1 is effective in inhibiting tive strain that exhibits activity against insects, mites, nema growth of plant pathogenic bacteria and fungi. todes and/or phytopathogens. 0034) Bacillus thuringiensis BD#32 (NRRL Accession 0043. In another preferred embodiment the composition No. B-21530) (in the following sometimes referred to as B11) of the present invention is characterized in that the biological exhibits insecticidal activity (U.S. Pat. No. 5,645,831 A). It control agent is selected from the group consisting of Bacillus produces a non-exotoxin, solvent-extractable, non-proteina subtilis AQ30002 (also known as QST30002) (NRRL Acces ceous metabolite that is 100% effective in killing corn root sion No. B-50421), Bacillus subtilis AQ30004 (also known as worm. The biopesticide produced by this bacterial strain is QST30004) (NRRL Accession No. B-50455, or a Bacillus subtilis Strain having a mutation in the Swra gene that results active against corn rootworm but inactive against flies. in impaired Swarming ability and enhanced plant health pro 0035. According to WO 98/21965 A1 the antibiotic pro motion compared to a strain containing a wildtype Swra ducing strain Bacillus thuringiensis AQ52 (NRRL Accession gene, and/or a mutant of these stains having all the identifying No. B-21619) (in the following sometimes referred to as B12) characteristics of the respective strain, and/or a metabolite exhibits broad fungicidal and bactericidal activity. produced by the respective Strain that exhibits activity against 0036 WO 02/02082898 A1 describes endophytic fungi insects, mites, nematodes and/or phytopathogens. including Muscodor albus 620, also known as Moscodor 0044. In another preferred embodiment the composition albus QST 20799 (NRRL Accession No. 30547) (in the fol of the present invention comprises a combination of at least lowing sometimes referred to as B13) and Muscodor roseus two biological control agents selected from the group con A3-5 (NRRL Accession No. 30548) (in the following some sisting of Bacillus chitinosporus AQ746 (NRRL Accession times referred to as B14) that produce a mixture of volatile No. B-21618), Bacillus mycoides AQ726 (NRRL Accession antibiotics with activity against fungi, bacteria, insects and No. B-21664), Bacillus pumilus (NRRL Accession No. nematodes. B-30087), Bacillus pumilus AQ717 (NRRL Accession No. 0037 Rhodococcus globerulus AQ719 (NRRL Accession B-21662), Bacillus sp. AQ175 (ATCC Accession No. 55608), No. B-21663) (in the following sometimes referred to as B15) Bacillus sp. AQ177 (ATCC Accession No. 55609), Bacillus produces metabolites that exhibits pesticidal activity against sp. AQ178 (ATCC Accession No. 53522), Bacillus subtilis corn rootworms (U.S. Pat. No. 6,027,723 A). AQ743 (NRRL Accession No. B-21665), Bacillus subtilis 0038 WO 01/79480A2 describes a strain of Streptomyces AQ713 (NRRL Accession No. B-21661), Bacillus subtilis gallbus (NRRL Accession No. 30232) (in the following some AQ153 (ATCC Accession No. 55614), Bacillus thuringiensis times referred to as B16) which shows insecticidal activity BDi32 (NRRL Accession No. B-21530), Bacillus thuring against Lepidoptera. iensis AQ52 (NRRL Accession No. B-21619), Muscodor 0039. The Streptomyces sp. strain described in WO albus 620 (NRRL Accession No. 30547), Muscodor roseus 02/26041 A2 (NRRL Accession No. B-301.45) (in the follow A3-5 (NRRL Accession No. 30548), Rhodococcus globeru ing sometimes referred to as B17) exhibits antifungalactivity lus AQ719 (NRRL Accession No. B-21663), Streptomyces US 2015/O 141245 A1 May 21, 2015 gallbus (NRRL Accession No. 30232), Streptomyces sp. epimeric enantiomer 1S,4R,9R), (F79) isopyrazam (syn. (NRRL Accession No. B-301.45), Bacillus thuringiensis sub epimeric racemate 1 RS4SR,9RS), (F80) isopyrazam (syn spec. kurstaki BMP 123, Bacillus subtilis AQ30002 (NRRL epimeric enantiomer 1R,4S.9R), (F81) isopyrazam (syn Accession No. B-50421), and Bacillus subtilis AQ 30004 epimeric enantiomer 1S,4R.9S), (F82) mepronil (55814-41 (NRRL Accession No. B-50455) and/or a mutant of these 0), (F83)oxycarboxin (5259-88-1), (F84) penflufen (494793 strains having all the identifying characteristics of the respec 67-8), (F85) penthiopyriad (183675-82-3), (F86) sedaxane tive strain, and/or a metabolite produced by the respective (874.967-67-6), (F87) thifluzamide (130000-40-7), (F88) strain that exhibits activity against insects, mites, nematodes 1-methyl-N-2-(1,1,2,2-tetrafluoroethoxyl)phenyl)-3-(trif and/or phytopathogens. luoromethyl)-1H-pyrazole-4-carboxamide, (F89) 3-(difluo 0045. According to one embodiment of the present inven romethyl)-1-methyl-N-2-(1,1,2,2-tetrafluoroethoxy)phe tion the biological control agent comprises not only the iso nyl)-1H-pyrazole-4-carboxamide, (F90) 3-(difluoromethyl)- lated, pure cultures of the respective microorganisms, but also N-4-fluoro-2-(1,1,2,3,3,3-hexafluoropropoxyl)phenyl)-1- their suspensions in a whole broth culture or a metabolite methyl-1H-pyrazole-4-carboxamide, (F91)N-1-(2,4- containing Supernatant orapurified metabolite obtained from dichlorophenyl)-1-methoxypropan-2-yl-3- whole broth culture of the Strain. “Whole broth culture’ refers (difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide to a liquid culture containing both cells and media. “Super (1092400-95-7), (F92) 5,8-difluoro-N-2-(2-fluoro-4-4- natant” refers to the liquid broth remaining when cells grown (trifluoromethyl)pyridin-2-yl)oxyphenyl)ethylquinazolin in broth are removed by centrifugation, filtration, sedimenta 4-amine (1210070-84-0), (F93) benzovindiflupyr. (F94)N- tion, or other means well known in the art. (1S,4R)-9-(dichloromethylene)-1,2,3,4-tetrahydro-1,4- 0046. The above-mentioned metabolites produced by the methanonaphthalen-5-yl)-3-(difluoromethyl)-1-methyl-1H nonpathogenic microorganisms include antibiotics, pyrazole-4-carboxamide, (F95)N-(1R,4S)-9- enzymes, siderophores and growth promoting agents, for (dichloromethylene)-1,2,3,4-tetrahydro-1,4- example Zwittermicin-A, kanosamine, polyoxine, enzymes methanonaphthalen-5-yl)-3-(difluoromethyl)-1-methyl-1H Such as C.-amylase, chitinases, and pektinases, phytohor pyrazole-4-carboxamide, (F96) 3-(Difluormethyl)-1- mones and precursors thereof. Such as auxines, gibberlin-like methyl-N-(1,1,3-trimethyl-2,3-dihydro-1H-inden-4-yl)-1H Substacnes, cytokinin-like compounds, lipopeptides Such as pyrazol-4-carboxamid, (F97) 1,3,5-Trimethyl-N-(1,1,3- iturins, plipastatins or Surfactins, e.g. agrastatin A, bacillo trimethyl-2,3-dihydro-1H-inden-4-yl)-1H-pyrazol-4- mycin D. bacilysin, difficidin, macrolactin, fengycin, bacil carboxamid, (F98) 1-Methyl-3-(trifluorimethyl)-N-(1,3,3- ysin and bacilaene. Preferred metabolites of the above listed trimethyl-2,3-dihydro-1H-inden-4-yl)-1H-pyrazol-4- are lipopeptides, in particular those produced by Bacillus carboxamid, (F99) 1-Methyl-3-(trifluorimethyl)-N-(1S)-1,3, pumilus (NRRL Accession No. B-30087) or Bacillus subtilis 3-trimethyl-2,3-dihydro-1H-inden-4-yl)-1H-pyrazol-4- AQ713 (NRRL Accession No. B-21661). Especially pre carboxamid, (F100) 1.-Methyl-3-(trifluorimethyl)-N-(1R)-1, ferred metabolites are Iturin A, Surfactin, Plipstatin and Agr 3,3-trimethyl-2,3-dihydro-1H-inden-4-yl)-1H-pyrazol-4- astatin A. An even more preferred metabolite is agrastatin A. carboxamid, (F101) 3-(Difluormethyl)-1-methyl-N-(3S)-1, 0047 According to the invention, the biological control 1,3-trimethyl-2,3-dihydro-1H-inden-4-yl)-1H-pyrazol-4- agent may be employed or used in any physiologic state Such carboxamid, (F102) 3-(Difluorimethyl)-1-methyl-N-(3R)-1, as active or dormant. 1,3-trimethyl-2,3-dihydro-1H-inden-4-yl)-1H-pyrazol-4- carboxamid, (F103) 1,3,5-Trimethyl-N-(3R)-1,1,3- Fungicide (I) trimethyl-2,3-dihydro-1H-inden-4-yl)-1H-pyrazol-4- 0048. In general, “fungicidal' means the ability of a sub carboxamid, (F104) 1,3,5-Trimethyl-N-((3S)-1,1,3- stance to increase mortality or inhibit the growth rate of fungi. trimethyl-2,3-dihydro-1H-inden-4-yl)-1H-pyrazol-4- 0049. The term “fungus' or “fungi' includes a wide vari carboxamid. ety of nucleated sporebearing organisms that are devoid of 0053 All named fungicides mentioned in this application chlorophyll. Examples of fungi include yeasts, molds, mil (i. e. F1 to F380) can, if their functional groups enable this, dews, rusts, and mushrooms. optionally form salts with suitable bases or acids. 0050. The composition according to the present invention 0054 According to a preferred embodiment of the present comprises at least one fungicide (I) which is selected from the invention fungicide (I) is selected from the group consisting group consisting of inhibitors of the respiratory chain at com of plex I or II. (2) inhibitors of the respiratory chain at complex I or II, for 0051 Preferably, the fungicide (I) does not have any fun example (F65) bixafen (581809-46-3), (F66) boscalid gicidal activity against any of the biological controla agents (188425-85-6), (F67) carboxin (5234-68-4), (F70) fluopyram of the invention. (658066-35-4), (F71) flutolanil (66332-96-5), (F72) fluxapy 0052 Preferably, the fungicide (I) is selected from the roxad (907204-31-3), (F73) furametpyr (123572-88-3), group consisting of (F75) isopyrazam (mixture of syn-epimeric racemate 1 (F65) bixafen (581809-46-3), (F66) boscalid (188425-85-6), RS4SR,9RS and anti-epimeric racemate 1 RS4SR.9SR) (F67)carboxin (5234-68-4), (F68) diflumetorim (130339-07 (881685-58-1), (F76) isopyrazam (anti-epimeric racemate 1 0), (F69) fenfuram (24691-80-3), (F70) fluopyram (658066 RS4SR.9SR), (F77) isopyrazam (anti-epimeric enantiomer 35-4), (F71) flutolanil (66332-96-5), (F72) fluxapyroxad 1R,4S,9S), (F78) isopyrazam (anti-epimeric enantiomer (907204-31-3), (F73) furametpyr (123572-88-3), (F74) fur 1S,4R,9R), (F79) isopyrazam (syn epimeric racemate 1RS, mecyclox (60568-05-0), (F75) isopyrazam (mixture of syn 4SR,9RS), (F80) isopyrazam (syn-epimeric enantiomer epimeric racemate 1 RS4SR,9RS and anti-epimeric race 1R,4S,9R), (F81) isopyrazam (syn-epimeric enantiomer mate 1 RS4SR.9SR) (881685-58-1), (F76) isopyrazam (anti 1S,4R,9S), (F84) penflufen (494793-67-8), (F85) penthiopy epimeric racemate 1 RS4SR.9SR), (F77) isopyrazam (anti rad (183675-82-3), (F86) sedaxane (874967-67-6), (F87) thi epimeric enantiomer 1R,4S.9S), (F78) isopyrazam (anti fluzamide (130000-40-7), (F91)N-1-(2,4-dichlorophenyl)- US 2015/O 141245 A1 May 21, 2015

1-methoxypropan-2-yl)-3-(difluoromethyl)-1-methyl-1H pyrazole-4-carboxamide (1092400-95-7), (F98) 1-Methyl-3- (trifluormethyl)-N-(1,3,3-trimethyl-2,3-dihydro-1H-inden 4-yl)-1H-pyrazol-4-carboxamid, (F99) 1-Methyl-3- (trifluormethyl)-N-(1S)-1,3,3-trimethyl-2,3-dihydro-1H inden-4-yl)-1H-pyrazol-4-carboxamid, (F100) 1-Methyl-3- (trifluormethyl)-N-(1R)-1,3,3-trimethyl-2,3-dihydro-1H inden-4-yl)-1H-pyrazol-4-carboxamid, (F101) 3-(Difluorimethyl)-1-methyl-N- (3S)-1,1,3-trimethyl-2,3- dihydro-1H-inden-4-yl)-1H-pyrazol-4-carboxamid, (F102) 3-(Difluorimethyl)-1-methyl-N- (3R)-1,1,3-trimethyl-2,3- dihydro-1H-inden-4-yl)-1H-pyrazol-4-carboxamid: 0055 According to another preferred embodiment of the present invention fungicide (I) is selected from the group consisting of (F70) fluopyram (658066-35-4), (F71) flutola nil (66332-96-5), (F72) fluxapyroxad (907204-31-3), and (F84) penflufen (494793-67-8). 0056. In one embodiment of the present invention, fungi cide (I), e.g., the fungicide for use in seed treatment is selected from the group consisting of Carboxin (F67), Fluxapyroxad (F72), Penflufen (F84), Sedaxane (F86. Compositions According to the Present Invention 0057 According to the present invention the composition comprises at least one biological control agent selected from the group consisting of Bacillus chitinosporus AQ746 (NRRL Accession No. B-21618), Bacillus mycoides AQ726 (NRRL Accession No. B-21664), Bacillus pumilus (NRRL Acces sion No. B-30087), Bacillus pumilus AQ717 (NRRL Acces sion No. B-21662), Bacillus sp. AQ175 (ATCC Accession No. 55608), Bacillus sp. AQ177 (ATCC Accession No. 55609), Bacillus sp. AQ178 (ATCC Accession No. 53522), Bacillus subtilis AQ743 (NRRL Accession No. B-21665), Bacillus subtilis AQ713 (NRRL Accession No. B-21661), Bacillus subtilis AQ153 (ATCC Accession No. 55614), Bacillus thuringiensis BD#32 (NRRL Accession No. B-21530), Bacillus thuringiensis AQ52 (NRRL Accession No. B-21619), Muscodor albus 620 (NRRL Accession No. 30547), Muscodor roseus A3-5 (NRRL Accession No. 30548), Rhodococcus globerulus AQ719 (NRRL Accession No. B-21663), Streptomyces galbus (NRRL Accession No. 30232), Streptomyces sp. (NRRL Accession No. B-301.45), Bacillus thuringiensis subspec. Kurstaki BMP 123, Bacillus subtilis AQ30002 (NRRL Accession No. B-50421), and Bacillus subtilis AQ30004 (NRRL Accession No. B-50455) and/or a mutant of these stains having all the identifying characteristics of the respective strain, and/or a metabolite produced by the respective Strain that exhibits activity against insects, mites, nematodes and/or phytopathogens and the at least one fungicide (I) as specified in claim 1 in a synergisti cally effective amount. 0058. A “synergistically effective amount according to the present invention represents a quantity of a combination of a biological control agent and a fungicide that is statisti cally significantly more effective against insects, mites, nematodes and/or phytopathogens than the biological control agent or the fungicide only. 0059. In a preferred embodiment the composition accord ing to the present invention comprises the following combi nations: B1+F65, B1+F66, B1+F67, B1+F68, B1+F69, B1+F70, B1+F71, B1+F72, B1+F73, B1+F74, B1+F75, B1+F76, B1+F77, B1+F78, B1+F79, B1+F80, B1+F81, B1+F82, B1+F83, B1+F84, B1+F85, B1+F86, B1+F87, B1+F88,

US 2015/O 141245 A1 May 21, 2015 methyl-4-3-(trimethylsilyl) propoxyphenyl-N-ethyl-N- (5) Compounds capable to have a multisite action, like for methylimidoformamide, (F63)N-ethyl-N-methyl-N'-(2- example (F152) bordeaux mixture (8011-63-0), (F153) methyl-5-(trifluoromethyl)-4-3-(trimethylsilyl)propoxy captafol (2425-06-1), (F154) captan (133-06-2), (F155) chlo phenyl)imidoformamide, (F64) 0-1-(4-methoxyphenoxy)- rothalonil (1897-45-6), (F156) copper hydroxide (20427-59 3.3-dimethylbutan-2-yl)1H-imidazole-1-carbothioate 2), (F157) copper naphthenate (1338-02-9). (F158) copper (111226-71-2); oxide (1317-39-1), (F159) copper oxychloride (1332-40-7), (F160) copper(2+) sulfate (7758-98-7), (F161) dichlofluanid (2) inhibitors of the respiratory chain at complex I or II as (1085-98-9), (F162) dithianon (3347-22-6), (F163) dodine explained before (provided that fungicide (I) and fungicide (2439-10-3), (F164) dodine free base, (F165)ferbam (14484 (II) are not identical): 64-1), (F166) fluorofolpet (719-96-0), (F167) folpet (133-07 (3) inhibitors of the respiratory chain at complex Ill, for 3), (F168) guazatine (108173-90-6), (F169) guazatine example (F105) ametoctradin (865318-97-4), (F106) amisul acetate, (F170) iminoctadine (13516-27-3), (F171) iminocta brom (348.635-87-0), (F107) azoxystrobin (131860-33-8), dine albesilate (169202-06-6), (F172) iminoctadine triacetate (F108) cyazofamid (1201 16-88-3), (F109) coumethox (57520-17-9), (F173) mancopper(53.988-93-5), (F174) man ystrobin (850881-30-0), (F110) coumoxystrobin (850881 cozeb (8018-01-7), (F175) maneb (12427–38-2), (F176) 70-8), (F111) dimoxystrobin (141600-52-4), (F112) enes metiram (9006-42-2), (F177) metiram zinc (9006-42-2), troburin (238410-11-2), (F113) famoxadone (131807-57-3), (F178) oxine-copper (10380-28-6), (F179) propamidine (F114) fenamidone (161326-34-7), (F115) fenoxystrobin (104-32-5), (F180) propineb (12071-83-9), (F181) sulphur (918162-02-4), (F116) fluoxastrobin (361377-29-9). (F117) and Sulphur preparations including calcium polysulphide kresoxim-methyl (143390-89-0), (F118) metominostrobin (7704-34-9), (F182) thiram (137-26-8), (F183) tolylfluanid (133408-50-1), (F119) orysastrobin (189892-69-1), (F120) (731-27-1), (F184) Zineb (12122-67-7), (F185) ziram (137 picoxystrobin (117428-22-5), (F121) pyraclostrobin 30-4); (175013-18-0), (F122) pyrametostrobin (915410-70-7), (6) Compounds capable to induce a host defense, like for (F123) pyraoxystrobin (862588-11-2), (F124) pyribencarb example (F186) acilbenzolar-S-methyl (135158-54-2), (799247-52-2), (F125) triclopyricarb (902760-40-1), (F126) (F187) isotia nil (224049-04-1), (F188) probenazole (27605 trifloxystrobin (141517-21-7), (F127) (2E)-2-(2-6-(3- 76-1), (F189) tiadinil (223580-51-6): chloro-2-methylphenoxy)-5-fluoropyrimidin-4-yl) (7) Inhibitors of the amino acid and/or protein biosynthesis, oxyphenyl)-2-(methoxyimino)-N-methylethanamide, for example (F190) andoprim (23951-85-1), (F191) blastici (F128) (2E)-2-(methoxyimino)-N-methyl-2-(2-({(1E)-1- din-S (2079-00-7), (F192) cyprodinil (121552-61-2), (F193) 3-(trifluoromethyl)phenylethylideneamino)oxy kasugamycin (6980-18-3), (F194) kasugamycin hydrochlo methylphenyl)ethanamide, (F129) (2E)-2-(methoxyimino)- ridehydrate (19408-46-9), (F195) mepanipyrim (110235-47 N-methyl-2-2-(E)-(1-3-(trifluoromethyl)phenyl 7), (F196) pyrimethanil (53112-28-0), (F197) 3-(5-fluoro-3, ethoxy)imino)methylphenylethanamide (158169-73-4), 3,4,4-tetramethyl-3,4-dihydroisoquinolin-1-yl)guinoline (F130) (2E)-2-2-((1E)-1-(3-(E)-1-fluoro-2-phe (86.1647-32-7); nylethenyloxyphenyl)ethylidenelamino oxy) methylphe (8) Inhibitors of the ATP production, for example (F198) nyl-2-(methoxyimino)-N-methylethanamide (326896-28 fentin acetate (900-95-8), (F199) fentin chloride (639-58-7), 0), (F131) (2E)-2-2-({(2E.3E)-4-(2,6-dichlorophenyl) (F200) fentin hydroxide (76-87-9), (F201) silthiofam but-3-en-2-ylidenelaminooxy)methylphenyl-2- (175217-20-6): (methoxyimino)-N-methylethanamide, (F132) 2-chloro-N- (9) Inhibitors of the cell wall synthesis, for example (F202) (1,1,3-trimethyl-2,3-dihydro-1H-inden-4-yl)pyridine-3- benthiavalicarb (177406–68-7), (F203) dimethomorph carboxamide (119899-14-8), (F133) 5-methoxy-2-methyl-4- (110488-70-5), (F204) flumorph (21 1867-47-9), (F205) (2-({(1E)-1-3-(trifluoromethyl)phenyl iprovalicarb (140923-17-7), (F206) mandipropamid ethylidene amino)oxymethylphenyl)-2,4-dihydro-3H-1, (374726-62-2), (F207) polyoxins (11113-80-7), (F208) poly 2,4-triazol-3-one, (F134) methyl (2E)-2-2-(cyclopropyl oxorim (22976-86-9), (F209) validamycin A (37248-47-8), (4-methoxyphenyl)iminomethylsulfanyl)methyl (F210) vali?enalate (283159-94-4: 283159-90-0); phenyl-3-methoxyprop-2-enoate (149601-03-6), (F135)N- (10) Inhibitors of the lipid and membrane synthesis, for (3-ethyl-3,5,5-trimethylcyclohexyl)-3-(formylamino)-2- example (F211) biphenyl (92-52-4), (F212) chloroneb (2675 hydroxybenzamide (226551-21-9). (F136) 2-2-(2,5- 77-6), (F213) dicloran (99-30-9), (F214) edifenphos (17109 dimethylphenoxy)methylphenyl)-2-methoxy-N- 49-8), (F215) etridiazole (2593-15-9), (F216) iodocarb methylacetamide (173662-97-0), (F137) (2R)-2-2-(2,5- (55406-53-6), (F217) iprobenfos (26087-47-8), (F218) iso dimethylphenoxy)methylphenyl)-2-methoxy-N- prothiolane (50512-35-1), (F219) propamocarb (25606-41 methylacetamide (394.657-24-0); 1), (F220) propamocarb hydrochloride (25606-41-1), (F221) (4) Inhibitors of the mitosis and cell division, for example prothiocarb (19622-08-3), (F222) pyrazophos (13457-18-6), (F138) benomyl (17804-35-2), (F139) carbendazim (10605 (F223) quintozene (82-68-8), (F224) tecnazene (117-18-0), 21-7), (F140) chlorfenazole (3574-96-7), (F141) diethofen (F225) tolclofos-methyl (57018-04-9); carb (87.130-20-9), (F142)ethaboxam (162650-77-3), (F143) (11) Inhibitors of the melanine biosynthesis, for example fluopicolide (2391 10-15-7), (F144) fuberidazole (3878-19 (F226) carpropamid (104030-54-8), (F227) diclocymet 1), (F145) pencycuron (66063-05-6), (F146) thiabendazole (139920-32-4), (F228) fenoxanil (115852-48-7), (F229) (148-79-8), (F147)thiophanate-methyl (23564-05-8), (F148) phthalide (27355-22-2), (F230) pyroquilon (57369-32-1), thiophanate (23564-06-9), (F149) Zoxamide (156052-68-5), (F231) tricyclazole (41814-78-2), (F232) 2.2.2-trifluoroethyl (F150) 5-chloro-7-(4-methylpiperidin-1-yl)-6-(2,4,6-trifluo {3-methyl-1-(4-methylbenzoyl)aminobutan-2- rophenyl) 1.2.4 triazolo 1.5-apyrimidine (214706-53-3), y1}carbamate (851524-22-6); (F151) 3-chloro-5-(6-chloropyridin-3-yl)-6-methyl-4-(2,4, (12) Inhibitors of the nucleic acid synthesis, for example 6-trifluorophenyl)pyridazine (1002756-87-7); (F233) benalaxyl (71626-11-4), (F234) benalaxyl-M (kiral

US 2015/O 141245 A1 May 21, 2015 or diammonium hydrogen phosphate, and/or retention pro phoxide, and also water. Preferred auxiliary solvents are moters such as dioctyl Sulphosuccinate or hydroxypropylguar selected from the group consisting of acetone and N,N'-dim polymers and/or humectants such as glycerol and/or fertiliz ethylacetamide. erS Such as ammonium, potassium orphosphorous fertilizers, 0080. In principle it is possible to use all suitable solvents. for example. Suitable solvents are, for example, aromatic hydrocarbons, 0074 Examples of typical formulations include water Such as Xylene, toluene or alkylnaphthalenes, for example, soluble liquids (SL), emulsifiable concentrates (EC), emul chlorinated aromatic oraliphatic hydrocarbons, such as chlo sions in water (EW), suspension concentrates (SC, SE, FS, robenzene, chloroethylene or methylene chloride, for OD), water-dispersible granules (WG), granules (GR) and example, aliphatic hydrocarbons, such as cyclohexane, for capsule concentrates (CS); these and other possible types of example, paraffins, petroleum fractions, mineral and Veg formulation are described, for example, by Crop Life Inter etable oils, alcohols, such as methanol, ethanol, isopropanol, national and in Pesticide Specifications, Manual on develop butanol or glycol, for example, and also their ethers and ment and use of FAO and WHO specifications for pesticides, esters, ketones such as acetone, methyl ethyl ketone, methyl FAO Plant Production and Protection Papers—173, prepared isobutyl ketone or cyclohexanone, for example, strongly by the FAO/WHO Joint Meeting on Pesticide Specifications, polar solvents, such as dimethyl Sulphoxide, and water. 2004, ISBN: 925.1048576. The formulations may comprise I0081 All suitable carriers may in principle be used. Suit active agrochemical compounds other than one or more active able carriers are in particular: for example, ammonium salts compounds of the invention. and ground natural minerals such as kaolins, clays, talc, 0075. The formulations or application forms in question chalk, quartz, attapulgite, montmorillonite or diatomaceous preferably comprise auxiliaries, such as extenders, solvents, earth, and ground synthetic minerals, such as finely divided spontaneity promoters, carriers, emulsifiers, dispersants, silica, alumina and natural or synthetic silicates, resins, waxes frost protectants, biocides, thickeners and/or other auxilia and/or solid fertilizers. Mixtures of such carriers may like ries, such as adjuvants, for example. An adjuvant in this wise be used. Carriers suitable for granules include the fol context is a component which enhances the biological effect lowing: for example, crushed and fractionated natural miner of the formulation, without the component itself having a als such as calcite, marble, pumice, Sepiolite, dolomite, and biological effect. Examples of adjuvants are agents which also synthetic granules of inorganic and organic meals, and promote the retention, spreading, attachment to the leaf Sur also granules of organic material Such as sawdust, paper, face, or penetration. coconut shells, maize cobs and tobacco stalks. 0.076 These formulations are produced in a known man I0082 Liquefied gaseous extenders or solvents may also be ner, for example by mixing the active compounds with aux used. Particularly suitable are those extenders or carriers iliaries such as, for example, extenders, solvents and/or Solid which at Standard temperature and under standard pressure carriers and/or further auxiliaries, such as, for example, Sur are gaseous, examples being aerosol propellants, such as factants. The formulations are prepared either in suitable halogenated hydrocarbons, and also butane, propane, nitro plants or else before or during the application. gen and carbon dioxide. 0.077 Suitable for use as auxiliaries are substances which are suitable for imparting to the formulation of the active I0083. Examples of emulsifiers and/or foam-formers, dis compound or the application forms prepared from these for persants or wetting agents having ionic or nonionic proper mulations (such as, e.g., usable crop protection agents. Such ties, or mixtures of these Surface-active Substances, are salts as spray liquors or seed dressings) particular properties Such of polyacrylic acid, salts of lignoSulphonic acid, salts of phe as certain physical, technical and/or biological properties. nolsulphonic acid or naphthalenesulphonic acid, polycon densates of ethylene oxide with fatty alcohols or with fatty 0078 Suitable extenders are, for example, water, polar and acids or with fatty amines, with substituted phenols (prefer nonpolar organic chemical liquids, for example from the ably alkylphenols or arylphenols), salts of Sulphosuccinic classes of the aromatic and non-aromatic hydrocarbons (such esters, taurine derivatives (preferably alkyltaurates), phos as paraffins, alkylbenzenes, alkylnaphthalenes, chloroben phoric esters of polyethoxylated alcohols or phenols, fatty Zenes), the alcohols and polyols (which, if appropriate, may acid esters of polyols, and derivatives of the compounds con also be substituted, etherified and/or esterified), the ketones taining Sulphates, Sulphonates and phosphates, examples (such as acetone, cyclohexanone), esters (including fats and being alkylaryl polyglycol ethers, alkylsulphonates, alkyl oils) and (poly)ethers, the unsubstituted and substituted Sulphates, arylsulphonates, protein hydrolysates, lignin-sul amines, amides, lactams (such as N-alkylpyrrolidones) and phite waste liquors and methylcellulose. The presence of a lactones, the Sulphones and Sulphoxides (such as dimethyl Surface-active Substance is advantageous if one of the active Sulphoxide). compounds and/or one of the inert carriers is not soluble in 0079 If the extender used is water, it is also possible to water and if application takes place in water. Preferred emul employ, for example, organic solvents as auxiliary Solvents. sifiers are alkylaryl polyglycol ethers. Essentially, Suitable liquid solvents are: aromatics such as Xylene, toluene or alkylnaphthalenes, chlorinated aromatics I0084. Further auxiliaries that may be present in the formu and chlorinated aliphatic hydrocarbons such as chloroben lations and in the application forms derived from them Zenes, chloroethylenes or methylene chloride, aliphatic include colorants such as inorganic pigments, examples being hydrocarbons such as cyclohexane or paraffins, for example iron oxide, titanium oxide, Prussian Blue, and organic dyes, petroleum fractions, mineral and vegetable oils, alcohols Such as alizarin dyes, azo dyes and metal phthalocyanine Such as butanol or glycol and also their ethers and esters, dyes, and nutrients and trace nutrients, such as salts of iron, ketones such as acetone, methyl ethylketone, methyl isobutyl manganese, boron, copper, cobalt, molybdenum and zinc. ketone or cyclohexanone, strongly polar solvents such as I0085 Stabilizers, such as low-temperature stabilizers, dimethylformamide, dimethylacetamide and dimethyl sul preservatives, antioxidants, light stabilizers or other agents US 2015/O 141245 A1 May 21, 2015

which improve chemical and/or physical stability may also be sp. AQ175 (ATCC Accession No. 55608), Bacillus sp. AQ177 present. Additionally present may be foam-formers or (ATCC Accession No. 55609), Bacillus sp. AQ178 (ATCC defoamers. Accession No. 53522), Bacillus subtilis AQ743 (NRRL I0086. Furthermore, the formulations and application Accession No. B-21665), Bacillus subtilis AQ713 (NRRL forms derived from them may also comprise, as additional Accession No. B-21661), Bacillus subtilis AQ153 (ATCC auxiliaries, stickers such as carboxymethylcellulose, natural Accession No. 55614), Bacillus thuringiensis BD#32 (NRRL and synthetic polymers in powder, granule or latex form, Such Accession No. B-21530), Bacillus thuringiensis AQ52 as gum arabic, polyvinyl alcohol, polyvinyl acetate, and also (NRRL Accession No. B-21619), Muscodor albus 620 natural phospholipids, such as cephalins and lecithins, and (NRRL Accession No. 30547), Muscodor roseus A3-5 synthetic phospholipids. Further possible auxiliaries include (NRRL Accession No. 30548), Rhodococcus globerulus mineral and vegetable oils. AQ719 (NRRL Accession No. B-21663), Streptomyces gal 0087. There may possibly be further auxiliaries present in bus (NRRL Accession No. 30232), Streptomyces sp. (NRRL the formulations and the application forms derived from Accession No. B-301.45), Bacillus thuringiensis subspec. them. Examples of such additives include fragrances, protec kurstaki BMP 123, Bacillus subtilis AQ30002 (NRRL Acces tive colloids, binders, adhesives, thickeners, thixotropic sub sion No. B-50421), and Bacillus subtilis AQ 30004 (NRRL stances, penetrants, retention promoters, stabilizers, seques Accession No. B-50455) and/or a mutant of these strains trants, complexing agents, humectants and spreaders. having all the identifying characteristics of the respective Generally speaking, the active compounds may be combined strain, and/or a metabolite produced by the respective strain with any solid or liquid additive commonly used for formu that exhibits activity against insects, mites, nematodes and/or lation purposes. phytopathogens and at least one fungicide (I) selected from 0088 Suitable retention promoters include all those sub the group consisting of inhibitors of the respiratory chain at stances which reduce the dynamic Surface tension, such as complex I or II in a synergistically effective amount, in a dioctyl Sulphosuccinate, or increase the Viscoelasticity, Such spatially separated arrangement. as hydroxypropylguar polymers, for example. 0093. In a further embodiment of the present invention the 0089 Suitable penetrants in the present context include all above-mentioned kit of parts further comprises at least one those Substances which are typically used in order to enhance additional fungicide (II), with the proviso that the biological the penetration of active agrochemical compounds into control agent, fungicide (I) and fungicide (II) are not identi plants. Penetrants in this context are defined in that, from the cal. Fungicide (II) can be present either in the biological (generally aqueous) application liquor and/or from the spray control agent component of the kit of parts or in the fungicide coating, they are able to penetrate the cuticle of the plant and (I) component of the kit of parts being spatially separated or thereby increase the mobility of the active compounds in the in both of these components. Preferably, fungicide (II) is cuticle. This property can be determined using the method present in the fungicide (I) component. described in the literature (Baur et al., 1997, Pesticide Science 0094 Moreover, the kit of parts according to the present 51, 131-152). Examples include alcohol alkoxylates such as invention can additionally comprise at least one auxiliary coconut fatty ethoxylate (10) or isotridecyl ethoxylate (12), selected from the group consisting of extenders, Solvents, fatty acid esters such as rapeseed or soybean oil methyl esters, spontaneity promoters, carriers, emulsifiers, dispersants, fatty amine alkoxylates such as tallowamine ethoxylate (15), frost protectants, thickeners and adjuvants as mentioned or ammonium and/or phosphonium salts such as ammonium below. This at least one auxiliary can be present either in the Sulphate or diammonium hydrogen phosphate, for example. biological control agent component of the kit of parts or in the 0090 The formulations preferably comprise between fungicide (I) component of the kit of parts being spatially 0.00000001% and 98% by weight of active compound or, separated or in both of these components. with particular preference, between 0.01% and 95% by 0095. In another aspect of the present invention the com weight of active compound, more preferably between 0.5% position as described above is used for reducing overall dam and 90% by weight of active compound, based on the weight age of plants and plant parts as well as losses in harvested of the formulation. The content of the active compound is fruits or vegetables caused by insects, mites, nematodes and/ defined as the Sum of the at least one biological control agent or phytopathogens. and the at least one fungicide (I). 0096. Furthermore, in another aspect of the present inven 0091. The active compound content of the application tion the composition as described above increases the overall forms (crop protection products) prepared from the formula plant health. tions may vary within wide ranges. The active compound 0097. The term “plant health’ generally comprises various concentration of the application forms may be situated typi sorts of improvements of plants that are not connected to the cally between 0.00000001% and 95% by weight of active control of pests. For example, advantageous properties that compound, preferably between 0.00001% and 1% by weight, may be mentioned are improved crop characteristics includ based on the weight of the application form. Application takes ing: emergence, crop yields, protein content, oil content, place in a customary manner adapted to the application forms. starch content, more developed root system, improved root growth, improved root size maintenance, improved root Kit of Parts effectiveness, improved stress tolerance (e.g. against drought, 0092. Furthermore, in one aspect of the present invention heat, salt, UV, water, cold), reduced ethylene (reduced pro a kit of parts is provided comprising at least one biological duction and/or inhibition of reception), tillering increase, control agent selected from the group consisting of Bacillus increase in plant height, bigger leaf blade, less dead basal chitinosporus AQ746 (NRRL Accession No. B-21618), leaves, stronger tillers, greener leaf color, pigment content, Bacillus mycoides AQ726 (NRRL Accession No. B-21664), photosynthetic activity, less input needed (such as fertilizers Bacillus pumilus (NRRL Accession No. B-30087), Bacillus or water), less seeds needed, more productive tillers, earlier pumilus AQ717 (NRRL Accession No. B-21662), Bacillus flowering, early grain maturity, less plant verse (lodging), US 2015/O 141245 A1 May 21, 2015

increased shoot growth, enhanced plant vigor, increased plant A3-5 (NRRL Accession No. 30548), Rhodococcus globeru stand and early and better germination. lus AQ719 (NRRL Accession No. B-21663), Streptomyces 0098. With regard to the use according to the present gallbus (NRRL Accession No. 30232), Streptomyces sp. invention, improved plant health preferably refers to (NRRL Accession No. B-30.145), Bacillus thuringiensis sub improved plant characteristics including: crop yield, more spec. kurstaki BMP 123, Bacillus subtilis AQ30002 (NRRL developed root system (improved root growth), improved Accession No. B-50421), and Bacillus subtilis AQ 30004 root size maintenance, improved root effectiveness, tillering (NRRL Accession No. B-50455) and/or a mutant of these increase, increase in plant height, bigger leafblade, less dead strains having all the identifying characteristics of the respec basal leaves, stronger tillers, greener leaf color, photosyn tive strain, and/or a metabolite produced by the respective thetic activity, more productive tillers, enhanced plant vigor, strain that exhibits activity against insects, mites, nematodes and increased plant stand. and/or phytopathogens and at least one fungicide (I) selected 0099. With regard to the present invention, improved plant from the group consisting of inhibitors of the respiratory health preferably especially refers to improved plant proper chain at complex I or II in a synergistically effective amount. ties selected from crop yield, more developed root system, 0105. In a preferred embodiment of the present method the improved root growth, improved root size maintenance, at least one fungicide (I) is selected from the group of fungi improved root effectiveness, tillering increase, and increase cides mentioned above. in plant height. 0106. In another preferred embodiment of the present 0100. The effect of a composition according to the present method the composition further comprises at least one addi invention on plant health health as defined herein can be tional fungicide (II), with the proviso that the biological con determined by comparing plants which are grown under the trol agent, fungicide (I) and fungicide (II) are not identical. same environmental conditions, whereby a part of said plants 0107 Preferably, the at least one additional fungicide (II) is treated with a composition according to the present inven is a synthetic fungicide. More preferably, fungicide (II) is tion and another part of said plants is not treated with a selected from the group of preferred fungicides (II) men composition according to the present invention. Instead, said tioned above. other part is not treated at all or treated with a placebo (i.e., an 0108. The method of the present invention includes the application without a composition according to the invention following application methods, namely both of the at least Such as an application without all active ingredients (i.e. one biological control agent and the at least one fungicide (I) without a biological control agent as described herein and mentioned before may be formulated into a single, stable without a fungicide as described herein), or an application composition with an agriculturally acceptable shelf life (so without a biological control agent as described herein, or an called “solo-formulation'), or being combined before or at application without a fungicide as described herein. the time of use (so called “combined-formulations'). 0101 The composition according to the present invention 0109 If not mentioned otherwise, the expression “combi may be applied in any desired manner, Such as in the form of nation' stands for the various combinations of the at least one a seed coating, Soil drench, and/or directly in-furrow and/or as biological control agent and the at least one fungicide (I), and a foliar spray and applied either pre-emergence, post-emer optionally the at least one fungicide (II), in a solo-formula gence or both. In other words, the composition can be applied tion, in a single “ready-mix' form, in a combined spray mix to the seed, the plant or to harvested fruits and vegetables or to ture composed from solo-formulations, such as a "tank-mix. the soil wherein the plant is growing or wherein it is desired to and especially in a combined use of the single active ingre grow (plant's locus of growth). dients when applied in a sequential manner, i.e. one after the 0102 Reducing the overall damage of plants and plant other within a reasonably short period, such as a few hours or parts often results in healthier plants and/or in an increase in days, e.g. 2 hours to 7 days. The order of applying the com plant vigor and yield. position according to the present invention is not essential for 0103 Preferably, the composition according to the present working the present invention. Accordingly, the term "com invention is used for treating conventional or transgenic bination' also encompasses the presence of the at least one plants or seed thereof. biological control agent and the at least one fungicide (I), and 0104. In another aspect of the present invention a method optionally the at least one fungicide (II) on or in a plant to be for reducing overall damage of plants and plant parts as well treated or its surrounding, habitat or storage space, e.g. after as losses in harvested fruits or vegetables caused by insects, simultaneously or consecutively applying the at least one mites, nematodes and/or phytopathogens is provided com biological control agent and the at least one fungicide (I), and prising the step of simultaneously or sequentially applying at optionally the at least one fungicide (II) to a plant its Sur least one biological control agent selected from the group rounding, habitat or storage space. consisting of Bacillus chitinosporus AQ746 (NRRL Acces 0110. If the at least one biological control agent and the at sion No. B-21618), Bacillus mycoides AQ726 (NRRL Acces least one fungicide (I), and optionally the at least one fungi sion No. B-21664), Bacillus pumilus (NRRL Accession No. cide (II) are employed or used in a sequential manner, it is B-30087), Bacillus pumilus AQ717 (NRRL Accession No. preferred to treat the plants or plant parts (which includes B-21662), Bacillus sp. AQ175 (ATCC Accession No. 55608), seeds and plants emerging from the seed), harvested fruits and Bacillus sp. AQ177 (ATCC Accession No. 55609), Bacillus Vegetables according to the following method: Firstly apply sp. AQ178 (ATCC Accession No. 53522), Bacillus subtilis ing the at least one fungicide (I) and optionally the at least one AQ743 (NRRL Accession No. B-21665), Bacillus subtilis fungicide (II) on the plant or plant parts, and secondly apply AQ713 (NRRL Accession No. B-21661), Bacillus subtilis ing the biological control agent to the same plant or plant AQ 153 (ATCC Accession No. 55614), Bacillus thuringiensis parts. The time periods between the first and the second BDi32 (NRRL Accession No. B-21530), Bacillus thuring application within a (crop) growing cycle may vary and iensis AQ52 (NRRL Accession No. B-21619), Muscodor depend on the effect to be achieved. For example, the first albus 620 (NRRL Accession No. 30547), Muscodor roseus application is done to prevent an infestation of the plant or US 2015/O 141245 A1 May 21, 2015

plant parts with insects, mites, nematodes and/or phytopatho depends on the final formulation as well as size or type of the gens (this is particularly the case when treating seeds) or to plant, plant parts, seeds, harvested fruit or vegetable to be combat the infestation with insects, mites, nematodes and/or treated. Usually, the fungicide (I) to be employed or used phytopathogens (this is particularly the case when treating according to the invention is present in about 0.1% to about plants and plant parts) and the second application is done to 80% (w/w), preferably 1% to about 60% (w/w), more pref. prevent or control the infestation with insects, mites, nema erably about 10% to about 50% (w/w) of its solo-formulation todes and/or phytopathogens. Control in this context means or combined-formulation with the biological control agent, that the biological control agent is not able to fully extermi and optionally the at least one fungicide (II). nate the pests orphytopathogenic fungi but is able to keep the 0116. The at least one biological control agent and the at infestation on an acceptable level. least one fungicide (I), and if present also the fungicide (II) By following the before mentioned steps, a very low level of are used or employed in a synergistic weight ratio. The skilled residues of the at least one fungicide (I), and optionally at person is able to find out the Synergistic weight ratios for the least one fungicide (II) on the treated plant, plant parts, and present invention by routine methods. The skilled person the harvested fruits and vegetables can beachieved. understands that these ratios refer to the ratio within a com 0111. If not mentioned otherwise the treatment of plants or bined-formulation as well as to the calculative ratio of the at plant parts (which includes seeds and plants emerging from least one biological control agent described herein and the the seed), harvested fruits and vegetables with the composi fungicide (I) when both components are applied as mono tion according to the invention is carried out directly or by formulations to a plant to be treated. The skilled person can action on their Surroundings, habitat or storage space using calculate this ratio by simple mathematics since the Volume customary treatment methods, for example dipping, spray and the amount of the biological control agent and fungicide ing, atomizing, irrigating, evaporating, dusting, fogging, (I), respectively, in a mono-formulation is known to the broadcasting, foaming, painting, spreading-on, watering skilled person. (drenching), drip irrigating. It is furthermore possible to 0117 The ratio can be calculated based on the amount of apply the at least one biological control agent, the at least one the at least one fungicide (I), at the time point of applying said fungicide (I), and optionally the at least one fungicide (II) as component of a combination according to the invention to a solo-formulation or combined-formulations by the ultra-low plant or plant part and the amount of a biological control agent Volume method, or to inject the composition according to the shortly prior (e.g., 48 h, 24h, 12 h, 6 h, 2 h, 1 h) or at the time present invention as a composition or as sole-formulations point of applying said component of a combination according into the soil (in-furrow). to the invention to a plant or plant part. 0112 The term “plant to be treated encompasses every 0118. The application of the at least one biological control part of a plant including its root system and the material—e. agent and the at least one fungicide (I) to a plant or a plant part g., soil or nutrition medium—which is in a radius of at least 10 can take place simultaneously or at different times as long as cm, 20 cm, 30 cm around the caulis or bole of a plant to be both components are present on or in the plant after the treated or which is at least 10 cm, 20 cm, 30 cm around the application(s). In cases where the biological control agent and root system of said plant to be treated, respectively. fungicide (I) are applied at different times and fungicide (I) is 0113. The amount of the biological control agent which is applied noticeable prior to the biological control agent, the used or employed in combination with the at least one fungi skilled person can determine the concentration of fungicide cide (I), optionally in the presence of at least one fungicide (I) on/in a plant by chemical analysis known in the art, at the (II), depends on the final formulation as well as size or type of time point or shortly before the time point of applying the the plant, plant parts, seeds, harvested fruits and vegetables to biological control agent. Vice versa, when the biological con be treated. Usually, the biological control agent to be trol agent is applied to a plant first, the concentration of a employed or used according to the invention is present in biological control agent can be determined using test which about 2% to about 80% (w/w), preferably in about 5% to are also known in the art, at the time point or shortly before the about 75% (w/w), more preferably about 10% to about 70% time point of applying fungicide (I). (w/w) of its solo-formulation or combined-formulation with 0119. In particular, in one embodiment the synergistic the at least one fungicide (I), and optionally the fungicide (II). weight ratio of the at least one biological control agent/spore 0114. In a preferred embodiment the biological control preparation and the at least fungicide (I) lies in the range of agent or e.g. their spores are present in a solo-formulation or 1:500 to 1000:1, preferably in the range of 1:500 to 500:1, the combined-formulation in a concentration of at least 10 more preferably in the range of 1:500 to 300:1. It has to be colony forming units per gram preparation (e. g. cells/g noted that these ratio ranges refer to the biological control preparation, spores/g preparation), such as 10-10" cfu/g, agent/spores preparation (to be combined with at least one preferably 10°-10'" cfu/g, more preferably 107-10' cfu/g fungicide (I) or a preparation of at least one fungicide (I)) of and most preferably 10-10" cfu/g at the time point of apply around 10" cells/spores per gram preparation of said cells/ ing biological control agents on a plant or plant parts such as spores. For example, a ratio of 100:1 means 100 weight parts seeds, fruits or vegetables. Also references to the concentra of a biological control agent/spore preparation having a cell/ tion of biological control agents in form of e.g., spores or spore concentration of 10" cells/spores per gram preparation cells—when discussing ratios between the amount of a prepa and 1 weight part of fungicide (I) are combined (either as a ration of at least one biological control agent and the amount Solo formulation, a combined formulation or by separate of fungicide (I)—are made in view of the time point when the applications to plants so that the combination is formed on the biological control agent is applied on a plant or plant parts plant). Such as seeds, fruits or vegetables. I0120 In another embodiment, the synergistic weight ratio 0115 Also the amount of the at least one fungicide (I) of the at least one biological control agent/spore preparation which is used or employed in combination with the biological to fungicide (I) is in the range of 1:100 to 20.000:1, preferably control agent, optionally in the presence of a fungicide (II), in the range of 1:50 to 10.000:1 or even in the range of 1:50 to US 2015/O 141245 A1 May 21, 2015

1000:1. Once again the mentioned ratios ranges refer to bio properties of pest-resistant or pest-tolerant transgenic plants, logical control agent/spore preparations of biological control in order to achieve optimum protection of the seed and of the agents of around 10" cells or spores per gram preparation of germinating plant with a minimal use of crop protection com said biological control agent. In particular, in this embodi positions. ment the biological control agent preferably is selected from I0127. The present invention therefore also relates in par the group consisting of Muscodor albus 620 (NRRL Acces ticular to a method for protecting seed and germinating plants sion No. 30547) and Muscodor roseus A3-5 (NRRL Acces from attack by pests, by treating the seed with at least one sion No. 30548). biological control agent as defined above and/or a mutant of it 0121 Still in another embodiment, the synergistic weight having all identifying characteristics of the respective strain, ratio of the at least one biological control agent/spore prepa and/or a metabolite produced by the respective strain that ration to the fungicide (I) is in the range of 1:0.0001 to 1:1, exhibits activity against insects, mites, nematodes and/or preferably in the range of 1:0.0005 to 1:0.5 or even in the phytopathogens and at least one fungicide (I) selected from range of 1:0.001 to 1:0.25. Here the mentioned ratio ranges the group consisting of inhibitors of the respiratory chain at refer to the amount in ppm of the BCA and the fungicide, complex I or II and optionally at least one fungicide (II) of the wherein the amount of the biological control agent refers to invention. The method of the invention for protecting seed the dried content of the BCA solution. In particular, in this and germinating plants from attack by pests encompasses a embodiment the biological control agent preferably is Bacil method in which the seed is treated simultaneously in one lus subtilis AQ30002 which is mentioned above as B19. In operation with the at least one biological control agent and the particular a solution of B19 is preferred which contains at least one fungicide (I), and optionally the at least one 1.34% of the BCA which refers to 8.5 0-10 CFU/g. Most fungicide(II). It also encompasses a method in which the seed preferably, when B19 is used as a BCA, the synergistic weight is treated at different times with the at least one biological ratio of at least B19 to the insecticide is selected from 1:0.008, control agent and the at least one fungicide (I), and optionally 1:0.004, 1:0.002, 1:0.02, and 1:0.2. the at least one fungicide (II). 0122) The cell/spore concentration of preparations can be 0128. The invention likewise relates to the use of the com determined by applying methods known in the art. To com position of the invention for treating seed for the purpose of pare weight ratios of the biological control agent/spore prepa protecting the seed and the resultant plant against insects, ration to fungicide (I), the skilled person can easily determine mites, nematodes and/or phytopathogens. the factor between a preparation having a biological control 0129. The invention also relates to seed which at the same agent/spore concentration different from 10" cells/spores per time has been treated with at least one biological control agent gram cell/spore preparation and a preparation having a bio and the at least one fungicide (I), and optionally at least one logical control agent/spore concentration of 10" cells/spores fungicide(II). The invention further relates to seed which has per gram preparation to calculate whether a ratio of a biologi been treated at different times with the at least one biological cal control agent/spore preparation to fungicide (I) is within control agent and the at least one fungicide (I) and optionally the scope of the above listed ratio ranges. the at least one fungicide (II). In the case of seed which has 0123. In one embodiment of the present invention, the been treated at different times with the at least one biological concentration of the biological control agent after dispersal is control agent and the at least one fungicide (I), and optionally at least 50 g/ha, such as 50-7500 g/ha, 50-2500 g/ha, 50-1500 the at least one fungicide(II), the individual active ingredients g/ha; at least 250 g/ha (hectare), at least 500 g/ha or at least in the composition of the invention may be present in different 800 g/ha. layers on the seed. 0.124. The application rate of composition to be employed 0.130. Furthermore, the invention relates to seed which, or used according to the present invention may vary. The following treatment with the composition of the invention, is skilled person is able to find the appropriate application rate Subjected to a film-coating process in order to prevent dust by way of routine experiments. abrasion of the seed. I0131 One of the advantages of the present invention is Seed Treatment that, owing to the particular systemic properties of the com 0.125. In another aspect of the present invention a seed positions of the invention, the treatment of the seed with these treated with the composition as described above is provided. compositions provides protection from insects, mites, nema 0126 The control of insects, mites, nematodes and/orphy todes and/or phytopathogens not only to the seed itself but topathogens by treating the seed of plants has been known for also to the plants originating from the seed, after they have a long time and is a Subject of continual improvements. Nev emerged. In this way, it may not be necessary to treat the crop ertheless, the treatment of seed entails a series of problems directly at the time of sowing or shortly thereafter. which cannot always be solved in a satisfactory manner. Thus, I0132 A further advantage is to be seen in the fact that, it is desirable to develop methods for protecting the seed and through the treatment of the seed with composition of the the germinating plant that remove the need for, or at least invention, germination and emergence of the treated seed significantly reduce, the additional delivery of crop protec may be promoted. tion compositions in the course of storage, after sowing or I0133. It is likewise considered to be advantageous com after the emergence of the plants. It is desirable, furthermore, position of the invention may also be used, in particular, on to optimize the amount of active ingredient employed in Such transgenic seed. a way as to provide the best-possible protection to the seed I0134. It is also stated that the composition of the invention and the germinating plant from attack by insects, mites, may be used in combination with agents of the signalling nematodes and/or phytopathogens, but without causing dam technology, as a result of which, for example, colonization age to the plant itself by the active ingredient employed. In with symbionts is improved. Such as rhizobia, mycorrhiza particular, methods for treating seed ought also to take into and/or endophytic bacteria, for example, is enhanced, and/or consideration the intrinsic insecticidal and/or nematicidal nitrogen fixation is optimized. US 2015/O 141245 A1 May 21, 2015

0135 The compositions of the invention are suitable for diluents, colorants, wetters, dispersants, emulsifiers, anti protecting seed of any variety of plant which is used in agri foams, preservatives, secondary thickeners, stickers, gibber culture, in greenhouses, in forestry or in horticulture. More ellins, and also water. particularly, the seed in question is that of cereals (e.g. wheat, 0.142 Colorants which may be present in the seed-dress barley, rye, oats and millet), maize, cotton, soybeans, rice, ing formulations which can be used in accordance with the potatoes, Sunflower, coffee, tobacco, canola, oilseed rape, invention include all colorants which are customary for Such beets (e.g. Sugar beet and fodder beet), peanuts, vegetables purposes. In this context it is possible to use not only pig (e.g. tomato, cucumber, bean, brassicas, onions and lettuce), ments, which are of low solubility in water, but also water fruit plants, lawns and ornamentals. Particularly important is soluble dyes. Examples include the colorants known under the treatment of the seed of cereals (such as wheat, barley, rye the designations Rhodamin B, C.I. Pigment Red 112 and C.I. and oats) maize, soybeans, cotton, canola, oilseed rape and Solvent Red 1. 1C. 0.143 Wetters which may be present in the seed-dressing 0136. As already mentioned above, the treatment of trans formulations which can be used in accordance with the inven genic seed with the composition of the invention is particu tion include all of the Substances which promote wetting and larly important. The seed in question here is that of plants which are customary in the formulation of active agrochemi which generally contain at least one heterologous gene that cal ingredients. Use may be made preferably of alkylnaph controls the expression of a polypeptide having, in particular, thalenesulphonates. Such as diisopropyl- or diisobutyl-naph insecticidal and/or nematicidal properties. These heterolo thalenesulphonates. gous genes in transgenic seed may come from microorgan 0144. Dispersants and/or emulsifiers which may be isms such as Bacillus, Rhizobium, Pseudomonas, Serratia, present in the seed-dressing formulations which can be used Trichoderma, Clavibacter; Glomus or Gliocladium. The in accordance with the invention include all of the nonionic, present invention is particularly suitable for the treatment of anionic and cationic dispersants that are customary in the transgenic seed which contains at least one heterologous gene formulation of active agrochemical ingredients. Use may be from Bacillus sp. With particular preference, the heterolo made preferably of nonionic or anionic dispersants or of gous gene in question comes from Bacillus thuringiensis. mixtures of nonionic or anionic dispersants. Suitable non 0.137 For the purposes of the present invention, the com ionic dispersants are, in particular, ethylene oxide-propylene position of the invention is applied alone or in a Suitable oxide block polymers, alkylphenol polyglycol ethers and also formulation to the seed. The seed is preferably treated in a tristryrylphenol polyglycol ethers, and the phosphated or Sul condition in which its stability is such that no damage occurs phated derivatives of these. Suitable anionic dispersants are, in the course of the treatment. Generally speaking, the seed in particular, lignoSulphonates, salts of polyacrylic acid, and may be treated at any point in time between harvesting and arylsulphonate-formaldehyde condensates. Sowing. Typically, seed is used which has been separated 0145 Antifoams which may be present in the seed-dress from the plant and has had cobs, hulls, stems, husks, hair or ing formulations which can be used in accordance with the pulp removed. Thus, for example, seed may be used that has invention include all of the foam inhibitors that are customary been harvested, cleaned and dried to a moisture content of less in the formulation of active agrochemical ingredients. Use than 15% by weight. Alternatively, seed can also be used that may be made preferably of silicone antifoams and magne after drying has been treated with water, for example, and sium Stearate. then dried again. 0146 Preservatives which may be present in the seed 0.138. When treating seed it is necessary, generally speak dressing formulations which can be used in accordance with ing, to ensure that the amount of the composition of the the invention include all of the substances which can be invention, and/or of other additives, that is applied to the seed employed for Such purposes in agrochemical compositions. is selected Such that the germination of the seed is not Examples include dichlorophen and benzyl alcohol hemifor adversely affected, and/or that the plant which emerges from mal. the seed is not damaged. This is the case in particular with 0147 Secondary thickeners which may be present in the active ingredients which may exhibit phytotoxic effects at seed-dressing formulations which can be used in accordance certain application rates. with the invention include all substances which can be used 0.139. The compositions of the invention can be applied for Such purposes in agrochemical compositions. Those con directly, in other words without comprising further compo templated with preference include cellulose derivatives, nents and without having been diluted. As a general rule, it is acrylic acid derivatives, Xanthan, modified clays and highly preferable to apply the compositions in the form of a suitable disperse silica. formulation to the seed. Suitable formulations and methods 0148 Stickers which may be present in the seed-dressing for seed treatment are known to the skilled person and are formulations which can be used in accordance with the inven described in, for example, the following documents: U.S. Pat. tion include all customary binders which can be used in No. 4,272,417 A, U.S. Pat. No. 4,245,432 A, U.S. Pat. No. seed-dressing products. Preferred mention may be made of 4,808,430 A, U.S. Pat. No. 5,876,739 A, US 2003/0176428 polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol A1, WO 2002/080675A1, WO 2002/028.186 A2. and tylose. 0140. The combinations which can be used in accordance 0149 Gibberellins which may be present in the seed with the invention may be converted into the customary seed dressing formulations which can be used in accordance with dressing formulations, such as Solutions, emulsions, Suspen the invention include preferably the gibberellins A1, A3 sions, powders, foams, slurries or other coating compositions (gibberellic acid), A4 and A7, with gibberellic acid being for seed, and also ULV formulations. used with particular preference. The gibberellins are known 0141. These formulations are prepared in a known man (cf. R. Wegler, “Chemie der Pflanzenschutz- and Schädlings ner, by mixing composition with customary adjuvants, such bekämpfungsmittel, Volume 2, Springer Verlag, 1970, pp. as, for example, customary extenders and also solvents or 401-412). US 2015/O 141245 A1 May 21, 2015

0150. The seed-dressing formulations which can be used Oligonychus spp., Ornithodorus spp., Ornithonyssus spp., in accordance with the invention may be used, either directly Panonychus spp., Phylocoptruta oleivora, Polyphagotarson or after prior dilution with water, to treat seed of any of a wide emus latus, Psoroptes spp., Rhipicephalus spp., Rhizoglyphus variety of types. Accordingly, the concentrates or the prepa spp., Sarcoptes spp., Scorpio maurus, Steneotarisonemus rations obtainable from them by dilution with water may be spp., Steneotarisonemus Spinki, Tarsonemus spp., Tetrany employed to dress the seed of cereals. Such as wheat, barley, chus spp., Trombicula alfreddugesi, Vaejovis spp., Vasates rye, oats and triticale, and also the seed of maize, rice, oilseed lycopersici; from the class Chilopoda, for example, Geophi rape, peas, beans, cotton, Sunflowers and beets, or else the lus spp., Scutigera spp.; seed of any of a very wide variety of vegetables. The seed from the order or the class Collembola, for example, Onychi dressing formulations which can be used in accordance with urus arnatus, the invention, or their diluted preparations, may also be used from the class Diplopoda, for example, Blaniulus guttulatus, to dress seed of transgenic plants. In that case, additional from the class Insecta, e.g. from the order Blattodea, for synergistic effects may occur in interaction with the Sub example, Blattella asahinai, Blattella germanica, Blatta ori stances formed through expression. entalis, Leucophaea maderae, Panchlora spp., Parcoblatta 0151. For the treatment of seed with the seed-dressing spp., Periplaneta spp., Supella longipalpa, formulations which can be used in accordance with the inven from the order Coleoptera, for example, Acalymma vittatum, tion, or with the preparations produced from them by addition Acanthoscelides Obtectus, Adoretus spp., Agelastica alni, of water, Suitable mixing equipment includes all such equip Agriotes spp., Alphitobius diaperinus, Amphimallon solsti ment which can typically be employed for seed dressing. tialis, Anobium punctatum, Anoplophora spp., Anthononus More particularly, the procedure when carrying out seed spp., Anthrenus spp., Apion spp., Apogonia spp., Atomaria dressing is to place the seed in a mixer, to add the particular spp., Attagenus spp., Bruchidius Obtectus, Bruchus spp., desired amount of seed-dressing formulations, either as Such Cassida spp., Cerotoma trifurcata, Ceutorrhynchus spp., or following dilution with water beforehand, and to carry out Chaetocnema spp., Cleonus mendicus, Conoderus spp., Cos mixing until the distribution of the formulation on the seed is mopolites spp., Costelytra zealandica, Ctenicera spp., Cur uniform. This may be followed by a drying operation. culio spp., Cryptolestes ferrugineus, Cryptorhynchus lap 0152 The application rate of the seed-dressing formula athi, Cylindrocopturus spp., Dermestes spp., Diabrotica spp., tions which can be used in accordance with the invention may Dichocrocis spp., Dicladispa armigera, Diloboderus spp., be varied within a relatively wide range. It is guided by the Epillachna spp., Epitrix spp., Faustinus spp., Gibbium psyl particular amount of the at least one biological control agent loides, Gnathocerus cornutus, Hellula undalis, Heteronychus and the at least one fungicide (I) in the formulations, and by arator, Heteronyx spp., Hylamorpha elegans, Hylotrupes the seed. The application rates in the case of the composition bajulus, Hypera postica, Hypomeces squamosus, Hypothen are situated generally at between 0.001 and 50g per kilogram emus spp., Lachnosterna consanguinea, Lasioderma serri of seed, preferably between 0.01 and 15 g per kilogram of corne, Latheticus Oryzae, Lathridius spp., Lema spp., Lepti seed. notarsa decemlineata, Leucoptera spp., Lissorhoptrus 0153. The composition according to the invention, in case Oryzophilus, Lixus spp., Luperodes spp., Lyctus spp., Megas the biological control agent exhibits insecticidal and nemati celis spp., Melanotus spp., Melligethes aeneus, Melolontha cidal activity, in combination with good plant tolerance and spp., Migdolus spp., Monochamus spp., Naupactus xan favourable toxicity to warm-blooded animals and being tol thographus, Necrobia spp., Niptus hololeucus, Oryctes rhi erated well by the environment, are suitable for protecting noceros, Oryzaephilus surinamensis, Oryzaphagus Oryzae, plants and plant organs, for increasing harvest yields, for Otiorrhynchus spp., Oxycetonia jucunda, Phaedon cochle improving the quality of the harvested material and for con ariae, Phyllophaga spp., Phyllophaga helleri, Phyllotreta trolling animal pests, in particular insects, arachnids, helm spp., Popillia japonica, Premnotrypes spp., ProStephanus inths, nematodes and molluscs, which are encountered in truncatus, Psylliodes spp., Ptinus spp., Rhizobius ventralis, agriculture, in horticulture, in animal husbandry, in forests, in Rhizopertha dominica, Sitophilus spp., Sitophilus Oryzae, gardens and leisure facilities, in protection of stored products Sphenophorus spp., Stegobium paniceum, Sternechus spp., and of materials, and in the hygiene sector. They can be Symphyletes spp., Tanymecus spp., Tenebrio molitor, Tene preferably employed as plant protection agents. In particular, brioides mauretanicus, Tribolium spp., Trogoderma spp., the present invention relates to the use of the composition Tvchius spp., Xvlotrechus spp., Zabrus spp.; according to the invention as insecticide and/or fungicide. from the order Diptera, for example, Aedes spp., Agromyza 0154 They are active against normally sensitive and resis spp., Anastrepha spp., Anopheles spp., Asphondylia spp., tant species and against all or some stages of development. Bactrocera spp., Bibio hortulanus, Caliphora erythro The abovementioned pests include: cephala, Caliphora vicina, Ceratitis capitata, Chironomus 0155 pests from the phylum Arthropoda, especially from spp., Chrysomyia spp., Chrysops spp., Chrysozona pluvialis, the class Arachnida, for example, Acarus spp., Aceria shel Cochliomyia spp., Contarinia spp., Cordylobia anthro doni, Aculops spp., Aculus spp., Amblyomma spp., Amphitet pophaga, Cricotopus Sylvestris, Culex spp., Culicoides spp., ranychus viennensis, Argas spp., Boophilus spp., Brevipalpus Culiseta spp., Cuterebra spp., Dacus oleae, Dasy neura spp., spp., Bryobia graminum, Bryobia praetiosa, Centruroides Delia spp., Dermatobia hominis, Drosophila spp., Echinoc spp., Chorioptes spp., Dermanyssus gallinae, Dermatopha nemus spp., Fannia spp., Gasterophilus spp., Glossina spp., goides pteronyssinus, Dermatophagoides farinae, Derma Haematopota spp., Hydrellia spp., Hydrellia griseola, Hyl centor spp., Eotetranychus spp., Epitrimerus pyri, Eutetrany emya spp., Hippobosca spp., Hypoderma spp., Liriomyza chus spp., Eriophyes spp., Glycyphagus domesticus, spp., Lucilia spp., Lutzomyia spp., Mansonia spp., Musca Halotydeus destructor; Hemitarisonemus spp., Hyalomma spp., Oestrus spp., Oscinella frit, Paratanytarsus spp., Para spp., Ixodes spp., Latrodectus spp., Loxosceles spp., Metatet lauterborniella subcincta, Pegomyia spp., Phlebotomus spp., ranychus spp., Neutrombicula autumnalis, Nuphersa spp., Phorbia spp., Phormia spp., Piophila casei, Prodiplosis spp., US 2015/014 1245 A1 May 21, 2015

Psila rosae, Rhagoletis spp., Sarcophaga spp., Simulium Lasius spp., Monomorium pharaonis, Sirex spp., Solenopsis spp., Stomoxys spp., Tabanus spp., Tetanops spp., Tipula spp. invicta, Tapinoma spp., Urocerus spp., Vespa spp., Xeris spp.; from the order Isopoda, for example, Armadillidium vulgare, from the order Heteroptera, for example, Anasa tristis, Antes Oniscus asellus, Porcellio scaber, tiopsis spp., Boisea spp., Blissus spp., Calocoris spp., from the order Isoptera, for example, Coptotermes spp., Cor Campylomma livida, Cavelerius spp., Cimex spp., Collaria nitermes cumulans, Cryptotermes spp., Incisitermes spp., spp., Creontiades dilutus, Dasynus piperis, Dichelops furca Microtermes obesi, Odontotermes spp., Reticulitermes spp.; tus, Diconocoris hewetti, Dysdercus spp., Euschistus spp., from the order Lepidoptera, for example, Achroia grisella, Eurygaster spp., Heliopeltis spp., Horcias nobilellus, Lepto Acronicta major, Adoxophyes spp., Aedia leucomelas, Agrotis corisa spp., Leptocorisa varicornis, Leptoglossus phyllopus, spp., Alabama spp., Amyelois transitella, Anarsia spp., Anti Lygus spp., Macropes excavatus, Miridae, Monallonion atra carsia spp., Argyroploce spp., Barathra brassicae, Borbo tum, Nezara spp., Oebalus spp., Pentomidae, Piesma cinnara, Bucculatrix thurberiella, Bupalus piniarius, quadrata, Piezodorus spp., Psallus spp., Pseudacy.sta persea, Busseola spp., Cacoecia spp., Caloptilia theivora, Capua Rhodnius spp., Sahlbergella singularis, Scaptocoris Casta reticulana, Carpocapsa pomonella, Carposina niponensis, nea, Scotinophora spp., Stephanitis nashi, Tibraca spp., Tri Cheimatobia brumata, Chilo spp., Choristoneura spp., Cly atoma spp.; sia ambiguella, Cnaphalocerus spp., Cnaphalocrocis medi from the order Homoptera, for example, Acizzia acaciae nalis, Cnephasia spp., Conopomorpha spp., Conotrachelus baileyanae, Acizzia dodonaeae, Acizzia uncatoides, Acrida spp., Copitarsia spp., Cydia spp., Dalaca noctuides, turrita, Acyrthosipon spp., Acrogonia spp., Aeneolamia spp., Diaphania spp., Diatraea saccharalis, Earias spp., Ecdytolo Agonoscena spp., Aleyrodes proletella, Aleurolobus baroden pha aurantium, Elasmopalpus lignosellus, Eldana saccha sis, Aleurothrixus floccosus, Allocaridara malayensis, rina, Ephestia spp., Epinotia spp., Epiphyas postvittana, Amrasca spp., Anuraphis cardui, Aonidiella spp., Apha Etiella spp., Eulia spp., Eupoecilia ambiguella, Euproctis nostigma piri, Aphis spp., Arboridia apicalis, Arytainilla spp., Euxoa spp., Feltia spp., Galleria mellonella, Gracillaria spp., Aspidiella spp., Aspidiotus spp., Atanus spp., Aula spp., Grapholitha spp., Hedylepta spp., Helicoverpa spp., corthum solani, Bemisia tabaci, Blastopsylla occidentalis, Heliothis spp., Hofmannophila pseudospretella, Homoeo Boreioglycaspis melaleucae, Brachycaudus helichrysi, soma spp., Homona spp., Hyponomeuta padella, Kakivoria Brachycolus spp., Brevicoryne brassicae, Cacopsylla spp., flavofasciata, Laphygma spp., Laspeyresia molesta, Leucino Calligpona marginata, Carneocephala fulgida, Ceratova des orbonalis, Leucoptera spp., Lithocolletis spp., Litho cuna lanigera, Cercopidae, Ceroplastes spp., Chaetosiphon phane antennata, Lobesia spp., Loxagrotis albicosta, Lyman fragaefolii, Chionaspis tegalensis, Chlorita Onuki, Chon tria spp., Lyonetia spp., Malacosoma neustria, Maruca dracris rosea, Chromaphis juglandicola, Chrysomphalus testulalis, Mamstra brassicae, Melanitis leda, Mocis spp., ficus, Cicadulina mbila, Coccomytilus halli, Coccus spp., Monopis obviella, Mythimna separata, Nemapogon cloacel Cryptomyzus ribis, CryptoneOssa spp., Ctenarytaina spp., lus, Nymphula spp., Oiketicus spp., Oria spp., Orthaga spp., Dalbulus spp., Dialeurodes citri, Diaphorina citri, Diaspis Ostrinia spp., Oulema oryzae, Panolis flammea, Parnara spp., Drosicha spp., Dysaphis spp., DysmicOccus spp., spp., Pectinophora spp., Perileucoptera spp., Phthorimaea Empoasca spp., Eriosoma spp., Erythroneura spp., Eucalyp spp., Phyllocnistis citrella, Phyllonorycter spp., Pieris spp., tolyma spp., Euphyllura spp., Euscelis bilobatus, Ferrisia Platynota Stultana, Plodia interpunctella, Plusia spp., Plu spp., Geococcus coffeae, Glycaspis spp., Heteropsylla tella xylostella, Prays spp., Prodenia spp., Protoparce spp., cubana, Heteropsylla spinulosa, Homalodisca Coagulata, Pseudaletia spp., Pseudaletia unipuncta, Pseudoplusia Hyalopterus arundinis, Icerya spp., Idiocerus spp., IdioSCO includens, Pyrausta nubilalis, Rachiplusia nu, Schoenobius pus spp., Laodelphax striatellus, Lecanium spp., Lepi spp., Scirpophaga spp., Scirpophaga innotata, Scotia Seg dosaphes spp., Lipaphis erysimi, Macrosiphum spp., Macros etum, Sesamia spp., Sesamia inferens, Sparganothis spp., teles facifions, Mahanarva spp., Melanaphis sacchari, Spodoptera spp., Spodoptera praefica, Stathmopoda spp., Metcalfiella spp., Metopolophium dirhodium, Monellia cos Stomopteryx subsecivella, Synanthedon spp., Tecia talis, Monelliopsis pecanis, Myzus spp., Nasonovia ribisini solanivora, Thermesia gemmatalis, Tinea cloacella, Tinea gri, Nephotettix spp., Nettigonicla spectra, Nilaparvata pellionella, Tineola bisselliella, Tortrix spp., Trichophaga lugens, Oncometopia spp., Orthezia praelonga, Oxya Chin tapetzella, Trichoplusia spp., Tryporyza incertulas, Titta ensis, Pachypsylla spp., Parabemisia myricae, Paratrioza absoluta, Virachola spp.; spp., Parlatoria spp., Pemphigus spp., Peregrinus maidis, from the order Orthoptera or Saltatoria, for example, Acheta Phenacoccus spp., Phloeomyzus passerinii, Phorodon domesticus, Dichroplus spp., Gryllotalpa spp., Hieroglyphus humuli, Phylloxera spp., Pinnaspis aspidistrae, Planococcus spp., Locusta spp., Melanoplus spp., Schistocerca gregaria, spp., Prosopidopsylla flava, Protopulvinaria pyriformis, from the order Phthiraptera, for example, Damalinia spp., Pseudaulacaspis pentagona, Pseudococcus spp., Psyllopsis Haematopinus spp., Linognathus spp., Pediculus spp., Ptirus spp., Psylla spp., Pteromalus spp., Pyrilla spp., Quadraspid pubis, Trichodectes spp.; iotus spp., Quesada gigas, Rastrococcus spp., Rhopalosi from the order Psocoptera for example Lepinatus spp., phum spp., Saissetia spp., Scaphoideus titanus, Schizaphis Liposcelis spp.; graminum, Selenaspidus articulatus, Sogata spp., Sogatella from the order Siphonaptera, for example, Ceratophyllus fircifera, Sogatodes spp., Stictocephala festina, Siphoninus spp., Ctenocephalides spp., Pulex irritans, Tunga penetrans, philly reae, Tenalaphara malavensis, Tetragonocephela spp., Xenopsylla cheopsis, Tinocallis caryaefoliae, Tomaspis spp., Toxoptera spp., Tri from the order Thysanoptera, for example, Anaphothrips aleurodes vaporariorum, Trioza spp., Tiphlocyba spp., Unas obscurus, Baliothrips biformis, Drepanothrips reuteri, pis spp., Viteus vitifolii, Zygina spp.; Enneothrips flavens, Frankliniella spp., Heliothrips spp., from the order Hymenoptera, for example, Acromyrmex spp., Hercinothrips femoralis, Rhipiphorothrips cruentatus, Scir Athalia spp., Atta spp., Diprion spp., Hoplocampa spp., tothrips spp., Taeniothrips cardamomi, Thrips spp.; US 2015/O 141245 A1 May 21, 2015 from the order Zygentoma (Thysanura), for example, seed dressing or soil fungicide. Furthermore, they are Suitable Ctenolepisma spp., Lepisma saccharina, Lepismodes inquili for combating fungi, which interalia infest wood or roots of nus, Thermobia domestica, plant. from the class Symphyla, for example, Scutigerella spp.; 0160 Bactericides can be used in crop protection for con pests from the phylum Mollusca, especially from the class trol of Pseudomonadaceae, Rhizobiaceae, Enterobacteri Bivalvia, for example, Dreissena spp., and from the class aceae, Corynebacteriaceae and Streptomycetaceae. Gastropoda, for example, Anion spp., Biomphalaria spp., 0.161 Non-limiting examples of pathogens of fungal dis Bulinus spp., Deroceras spp., Galba spp., Lynnaea spp., eases which can be treated in accordance with the invention Oncomelania spp., Pomacea spp., Succinea spp.; include: animal pests from the phylums Plathelminthes and Nema diseases caused by powdery mildew pathogens, for example toda, for example, Ancylostoma duodenale, Ancylostoma Blumeria species, for example Blumeria graminis, ceylanicum, Acylostoma braziliensis, Ancylostoma spp., Podosphaera species, for example Podosphaera leucotricha, Ascaris spp., Brugia malayi, Brugia timori, Bunostomum Sphaerotheca species, for example Sphaerotheca fitliginea, spp., Chabertia spp., Clonorchis spp., Cooperia spp., Dicro Uncinula species, for example Uncinula necator, coelium spp., Dictyocaulus filaria, Diphyllobothrium latum, diseases caused by rust disease pathogens, for example Gym Dracunculus medimensis, Echinococcus granulosus, Echino nosporangium species, for example Gymnosporangium sabi coccus multilocularis, Enterobius vermicularis, Faciola spp., nae, Hemileia species, for example Hemileia vastatrix, Pha Haemonchus spp., Heterakis spp., Hymenolepis nana, Hvos kopsora species, for example Phakopsora pachyrhizi and trongulus spp., Loa Loa, Nematodirus spp., Oesophagosto Phakopsora meibomiae, Puccinia species, for example Puc mum spp., Opisthorchis spp., Onchocerca volvulus, Osterta cinia recondite, P triticina, P. graminis or P. Striiformis, gia spp., Paragonimus spp., Schistosomen spp., Uromyces species, for example Uromyces appendiculatus, Strongyloides fuelleborni, Strongyloides Stercoralis, Strony diseases caused by pathogens from the group of the loides spp., Taenia Saginata, Taenia Solium, Trichinella spi Oomycetes, for example Albugo species, for example Algubo ralis, Trichinella nativa, Trichinella britovi, Trichinella nel candida, Bremia species, for example Bremia lactucae, Per soni, Trichinella pseudopsiralis, Tricho strongulus spp., onospora species, for example Peronospora pisi or P brassi Trichuris trichiura, Wuchereria bancrofti, cae, Phytophthora species, for example Phytophthora phytoparasitic pests from the phylum Nematoda, for infestans, Plasmopara species, for example Plasmopara viti example, Aphelenchoides spp., Bursaphelenchus spp., Dity cola, Pseudoperonospora species, for example Pseudoper lenchus spp., Globodera spp., Heterodera spp., Longidorus onospora humuli or Pseudoperonospora cubensis, Pythium spp., Meloidogyne spp., Pratylenchus spp., Radopholus spp., species, for example Pythium ultimum, Trichodorus spp., Tvlenchulus spp., Xiphinema spp., Heli leaf blotch diseases and leaf wilt diseases caused, for cotylenchus spp., Tilenchorhynchus spp., Scutellonema spp., example, by Alternaria species, for example Alternaria Paratrichodorus spp., Meloinema spp., Paraphelenchus spp., Solani; Cercospora species, for example Cercospora beti Agilenchus spp., Belonolaimus spp., Nacobbus spp., Rotylen cola, Cladiosporium species, for example Cladiosporium chulus spp., Rotylenchus spp., Neotylenchus spp., Paraph cucumerinum, Cochliobolus species, for example elenchus spp., Dolichodorus spp., Hoplolaimus spp., Punc Cochliobolus sativus (conidia form: Drechslera, Syn: Helm todera spp., Criconemella spp., Ouinisulcius spp., inthosporium), Cochliobolus miyabeanus, Colletotrichum Hemicycliophora spp., Anguina spp., Subanguina spp., species, for example Colletotrichum lindemuthanium, Cyclo Hemicriconenoides spp., Psilenchus spp., Pseudohalenchus conium species, for example Cycloconium oleaginum, spp., Criconenoides spp., Cacopaurus spp., Hirschmaniella Diaporthe species, for example Diaporthe citri, Elsinoe spe spp., Tetvlenchus spp. cies, for example Elsinoe fawcetti; Gloeosporium species, 0156. It is furthermore possible to control organisms from for example Gloeosporium laeticolor, Glomerella species, the subphylum Protozoa, especially from the order Coccidia, for example Glomerella cingulata, Guignardia species, for Such as Eimeria spp. example Guignardia bidwelli; Leptosphaeria species, for 0157. The present composition preferably is active against example Leptosphaeria maculans, Leptosphaeria nodorum, Phytophthora infestans, Venturia inaequalis, Sphaerotheca Magnaporthe species, for example Magnaporthe grisea, fiuliginea, Alternaria Solani, and/or Botrytis cinerea. Further Microdochium species, for example Microdochium nivale, more, the composition according to the present invention Mycosphaerella species, for example Mycosphaerella preferably has potent microbicidal activity and can be used graminicola, M. arachidicola and M. fijiensis, for control of unwanted microorganisms, such as fungi and Phaeosphaeria species, for example Phaeosphaeria bacteria, in crop protection and in the protection of materials. nodorum, Pyrenophora species, for example Pyrenophora 0158. The invention also relates to a method for control teres, Pyrenophora tritici repentis, Ranularia species, for ling unwanted microorganisms, characterized in that the example Ranularia collo-Cygni, Ranularia areola; Rhyncho inventive composition is applied to the phytopathogenic sporium species, for example Rhynchosporium secalis, Sep fungi, phytopathogenic bacteria and/or their habitat. toria species, for example Septoria apii, Septoria lycopersii; 0159 Fungicides can be used in crop protection for con Tiphula species, for example Tiphula incarnata, Venturia trol of phytopathogenic fungi. They are characterized by an species, for example Venturia inaequalis, outstanding efficacy against abroad spectrum of phytopatho root and stem diseases caused, for example, by Corticium genic fungi, including Soilborne pathogens, which are in par species, for example Corticium graminearum, Fusarium spe ticular members of the classes Plasmodiophoromycetes, Per cies, for example Fusarium oxysporum, Gaeumannomyces onosporomycetes (Syn. Oomycetes), Chytridiomycetes, species, for example Gaeumannomyces graminis, Rhizocto Zygomycetes, Ascomycetes, Basidiomycetes and Deutero nia species, such as, for example Rhizoctonia Solani; Saro mycetes (Syn. Fungi imperfecti). Some fungicides are sys ciadium diseases caused for example by Sarociadium Oryzae, temically active and can be used in plant protection as foliar, Sclerotium diseases caused for example by Sclerotium US 2015/O 141245 A1 May 21, 2015 20

Oryzae, Tapesia species, for example Tapesia acuformis, Ganoderma bominense, Rigidoporus diseases caused for Thielaviopsis species, for example Thielaviopsis basicola, example by Rigidoporus lignosus, ear and panicle diseases (including corn cobs) caused, for diseases of flowers and seeds caused, for example, by Botrytis example, by Alternaria species, for example Alternaria spp.; species, for example Botrytis cinerea, Aspergillus species, for example Aspergillus flavus, Cla diseases of plant tubers caused, for example, by Rhizoctonia dosporium species, for example Cladosporium cladosporio species, for example Rhizoctonia Solani; Helminthosporium ides, Claviceps species, for example Claviceps purpurea, species, for example Helminthosporium Solani; Fusarium species, for example Fusarium culmorum, Gibber Club root caused, for example, by Plasmodiophora species, ella species, for example Gibberella zeae, Monographella for example Plamodiophora brassicae, species, for example Monographella nivalis, Septoria spe diseases caused by bacterial pathogens, for example Xanth cies, for example Septoria nodorum, Omonas species, for example Xanthomonas campestris pv. diseases caused by Smut fungi, for example Sphacelotheca Oryzae, Pseudomonas species, for example Pseudomonas species, for example Sphacelotheca reiliana, Tilletia species, syringae pv. lachrymans, Erwinia species, for example for example Tilletia caries, T. controversa, Urocystis species, Erwinia amylovora. for example Urocystis occulta, Ustilago species, for example 0162 The following diseases of soya beans can be con Ustilago nuda, U. nuda tritici; trolled with preference: fruit rot caused, for example, by Aspergillus species, for 0163 Fungal diseases on leaves, stems, pods and seeds example Aspergillus flavus, Botrytis species, for example caused, for example, by Alternaria leaf spot (Alternaria spec. Botrytis cinerea, Penicillium species, for example Penicil atrans tenuissima), Anthracnose (Colletotrichum gloeospor lium expansium and P. purpurogenium, Sclerotinia species, for Oides dematium var. truncatum), brown spot (Septoria gly example Sclerotinia sclerotiorum, Verticilium species, for cines), cercospora leaf spot and blight (Cercospora kikuchii), example Verticilium alboatrum, choanephora leaf blight (Choanephora infindibulifera seed and Soilborne decay, mould, wilt, rot and damping-off trispora (Syn.)), dactuliophora leaf spot (Dactuliophora gly diseases caused, for example, by Alternaria species, caused cines), downy mildew (Peronospora manshurica), drechslera for example by Alternaria brassicicola, Aphanomyces spe blight (Drechslera glycini), frogeye leaf spot (Cercospora cies, caused for example by Aphanomyces euteiches, Asco soina), leptosphaerulina leaf spot (Leptosphaerulina tri chyta species, caused for example by Ascochyta lentis, folii), phyllostica leaf spot (Phyllosticta sojaecola), pod and Aspergillus species, caused for example by Aspergillus fia stem blight (Phomopsis sojae), powdery mildew (Mi vus, Cladosporium species, caused for example by Cladospo Crosphaera diffusa), pyrenochaeta leaf spot (Pyrenochaeta rium herbarum, Cochliobolus species, caused for example by glycines), rhizoctonia aerial, foliage, and web blight (Rhizoc Cochliobolus sativus: (Conidiaform: Drechslera, Bipolaris tonia Solani), rust (Phakopsora pachyrhizi, Phakopsora mei Syn: Helminthosporium); Colletotrichum species, caused for bomiae), Scab (Sphaceloma glycines), Stemphylium leaf example by Colletotrichum coccodes, Fusarium species, blight (Stemphylium botryosum), target spot (Corynespora caused for example by Fusarium culmorum, Gibberella spe Cassicola). cies, caused for example by Gibberella zeae, Macrophomina 0164. Fungal diseases on roots and the stem base caused, species, caused for example by Macrophomina phaseolina, for example, by black root rot (Calonectria Crotalariae), Monographella species, caused for example by charcoal rot (Macrophomina phaseolina), fitsarium blight or Monographella nivalis, Penicillium species, caused for wilt, root rot, and pod and collar rot (Fusarium oxysporum, example by Penicillium expansium, Phoma species, caused Fusarium Orthoceras, Fusarium semitectum, Fusarium equi for example by Phoma lingam, Phomopsis species, caused setti), mycoleptodiscus root rot (Mycoleptodiscus terrestris), for example by Phomopsis sojae, Phytophthora species, neocosmospora (Neocosmospora vasinfecta), pod and stem caused for example by Phytophthora cactorum, Pyrenophora blight (Diaporthe phaseolorum), stem canker (Diaporthe species, caused for example by Pyrenophora graminea, phaseolorum var. caulivora), phytophthora rot (Phytophthora Pyricularia species, caused for example by Pyricularia megasperma), brown stem rot (Phialophora gregata), Oryzae, Pythium species, caused for example by Pythium pythium rot (Pythium aphanidermatum, Pythium irregulare, ultimum, Rhizoctonia species, caused for example by Rhizoc Pythium debaryanurn, Pythium myriotylum, Pythium ulti tonia Solani; Rhizopus species, caused for example by Rhizo mum), rhizoctonia root rot, stem decay, and damping-off pus Oryzae, Sclerotium species, caused for example by Scle (Rhizoctonia Solani), sclerotinia stem decay (Sclerotinia rotium rolfsii; Septoria species, caused for example by sclerotiorum), sclerotinia southern blight (Sclerotinia rolf Septoria nodorum, Tiphula species, caused for example by sii), thielaviopsis root rot (Thielaviopsis basicola). Tiphula incarnata, Verticillium species, caused for example 0.165. The inventive compositions can be used for curative by Verticillium dahliae, or protective/preventive control of phytopathogenic fungi. cancers, galls and witches broom caused, for example, by The invention therefore also relates to curative and protective Nectria species, for example Nectria galligena; methods for controlling phytopathogenic fungi by the use of wilt diseases caused, for example, by Monilinia species, for the inventive composition, which is applied to the seed, the example Monilinia laxa, plant or plant parts, the fruit or the soil in which the plants leaf blister or leaf curl diseases caused, for example, by grOW. Exobasidium species, for example Exobasidium vexans, 0166 The fact that the composition is well tolerated by Taphrina species, for example Taphrina deformans, plants at the concentrations required for controlling plant decline diseases of wooden plants caused, for example, by diseases allows the treatment of above-ground parts of plants, Esca disease, caused for example by Phaemoniella clamy of propagation stock and seeds, and of the soil. dospora, Phaeoacremonium aleophilum and Fomitiporia 0.167 According to the invention all plants and plant parts mediterranea, Eutypa dyeback, caused for example by can be treated. By plants is meant all plants and plant popu Eutylpa lata, Ganoderma diseases caused for example by lations such as desirable and undesirable wild plants, culti US 2015/O 141245 A1 May 21, 2015

vars and plant varieties (whether or not protectable by plant also stone fruits such as apricots, cherries, almonds, plums variety or plant breeder's rights). Cultivars and plant varieties and peaches, and berry fruits such as Strawberries, raspber can be plants obtained by conventional propagation and ries, red and black currant and gooseberry), Ribesioidae sp., breeding methods which can be assisted or Supplemented by Juglandaceae sp., Betulaceae sp., Anacardiaceae sp., one or more biotechnological methods such as by use of Fagaceae sp., Moraceae sp., Oleaceae sp. (e.g. olive tree), double haploids, protoplast fusion, random and directed Actinidaceae sp., Lauraceae sp. (e.g. avocado, cinnamon, mutagenesis, molecular or genetic markers or by bioengi camphor), Musaceae sp. (e.g. banana trees and plantations), neering and genetic engineering methods. By plant parts is Rubiaceae sp. (e.g. coffee), Theaceae sp. (e.g. tea), Stercu meant all above ground and below ground parts and organs of liceae sp., Rutaceae sp. (e.g. lemons, oranges, mandarins and plants such as shoot, leaf blossom and root, whereby for grapefruit); Solanaceae sp. (e.g. tomatoes, potatoes, peppers, example leaves, needles, stems, branches, blossoms, fruiting capsicum, aubergines, tobacco), Liliaceae sp., Compositae bodies, fruits and seed as well as roots, corms and rhizomes sp. (e.g. lettuce, artichokes and chicory—including root are listed. Crops and vegetative and generative propagating chicory, endive or common chicory), Umbelliferae sp. (e.g. material, for example cuttings, corms, rhizomes, runners and carrots, parsley, celery and celeriac), Cucurbitaceae sp. (e.g. seeds also belong to plant parts. cucumbers—including gherkins, pumpkins, watermelons, 0168 The inventive composition, when it is well tolerated calabashes and melons), Alliaceae sp. (e.g. leeks and onions), by plants, has favourable homeotherm toxicity and is well Cruciferae sp. (e.g. white cabbage, red cabbage, broccoli, tolerated by the environment, is suitable for protecting plants cauliflower, Brussels sprouts, pak choi, kohlrabi, radishes, and plant organs, for enhancing harvest yields, for improving horseradish, cress and chinese cabbage), Leguminosae sp. the quality of the harvested material. It can preferably be used (e.g. peanuts, peas, lentils and beans—e.g. common beans as crop protection composition. It is active against normally and broad beans), Chenopodiaceae sp. (e.g. Swiss chard, sensitive and resistant species and against all or some stages fodder beet, spinach, beetroot), Linaceae sp. (e.g. hemp), of development. Cannabeacea sp. (e.g. Cannabis), Malvaceae sp. (e.g. okra, 0169 Plants which can be treated in accordance with the cocoa), Papaveraceae (e.g. poppy), Asparagaceae (e.g. invention include the following main crop plants: maize, soya asparagus).; useful plants and ornamental plants in the garden bean, alfalfa, cotton, Sunflower, Brassica oil seeds such as and woods including turf lawn, grass and Stevia rebaudiana; Brassica napus (e.g. canola, rapeseed), Brassica rapa, B. and in each case genetically modified types of these plants. juncea (e.g. (field) mustard) and Brassica carinata, 0171 More preferably, plants which can be treated in Arecaceae sp. (e.g. oilpalm, coconut), rice, wheat, Sugar beet, accordance with the invention are selected from the group Sugar cane, oats, rye, barley, millet and Sorghum, triticale, consisting of apples, cucumbers, tomatoes, and beans. flax, nuts, grapes and vine and various fruit and vegetables 0172 Depending on the plant species or plant cultivars, from various botanic taxa, e.g. Rosaceae sp. (e.g. pome fruits their location and growth conditions (soils, climate, vegeta Such as apples and pears, but also stone fruits such as apricots, tion period, diet), using or employing the composition cherries, almonds, plums and peaches, and berry fruits such according to the present invention the treatment according to as strawberries, raspberries, red and black currant and goose the invention may also result in Super-additive (“synergistic') berry), Ribesioidae sp., Juglandaceae sp., Betulaceae sp., effects. Thus, for example, by using or employing inventive Anacardiaceae sp., Fagaceae sp., Moraceae sp., Oleaceae composition in the treatment according to the invention, sp. (e.g. olive tree), Actinidaceae sp., Lauraceae sp. (e.g. reduced application rates and/or a widening of the activity avocado, cinnamon, camphor), Musaceae sp. (e.g. banana spectrum and/or an increase in the activity better plant trees and plantations), Rubiaceae sp. (e.g. coffee), Theaceae growth, increased tolerance to high or low temperatures, sp. (e.g. tea), Sterculiceae sp., Rutaceae sp. (e.g. lemons, increased tolerance to drought or to water or soil salt content, oranges, mandarins and grapefruit); Solanaceae sp. (e.g. increased flowering performance, easier harvesting, acceler tomatoes, potatoes, peppers, capsicum, aubergines, tobacco), ated maturation, higher harvest yields, bigger fruits, larger Liliaceae sp., Compositae sp. (e.g. lettuce, artichokes and plant height, greenerleaf color, earlier flowering, higher qual chicory—including root chicory, endive or common ity and/or a higher nutritional value of the harvested products, chicory), Umbelliferae sp. (e.g. carrots, parsley, celery and higher Sugar concentration within the fruits, better storage celeriac), Cucurbitaceae sp. (e.g. cucumbers—including stability and/or processability of the harvested products are gherkins, pumpkins, watermelons, calabashes and melons), possible, which exceed the effects which were actually to be Alliaceae sp. (e.g. leeks and onions), Cruciferae sp. (e.g. expected. white cabbage, red cabbage, broccoli, cauliflower, Brussels 0173 At certain application rates of the inventive compo sprouts, pak choi, kohlrabi, radishes, horseradish, cress and sition in the treatment according to the invention may also chinese cabbage), Leguminosae sp. (e.g. peanuts, peas, lentils have a strengthening effect in plants. The defense system of and beans—e.g. common beans and broad beans), Chenopo the plant against attack by unwanted phytopathogenic fungi diaceae sp. (e.g. Swiss chard, fodderbeet, spinach, beetroot), and/or microorganisms and/or viruses is mobilized. Plant Linaceae sp. (e.g. hemp), Cannabeacea sp. (e.g. cannabis), strengthening (resistance-inducing) Substances are to be Malvaceae sp. (e.g. okra, cocoa), Papaveraceae (e.g. poppy), understood as meaning, in the present context, those Sub Asparagaceae (e.g. asparagus).; useful plants and ornamental stances or combinations of Substances which are capable of plants in the garden and woods including turf, lawn, grass and stimulating the defense system of plants in Such a way that, Stevia rebaudiana; and in each case genetically modified when Subsequently inoculated with unwanted phytopatho types of these plants. genic fungi and/or microorganisms and/or viruses, the treated 0170 Preferably, plants which can be treated in accor plants display a substantial degree of resistance to these phy dance with the invention are selected from the group consist topathogenic fungi and/or microorganisms and/or viruses, ing of fruit and vegetables from various botanic taxa, e.g. Thus, by using or employing composition according to the Rosaceae sp. (e.g. pome fruits such as apples and pears, but present invention in the treatment according to the invention, US 2015/O 141245 A1 May 21, 2015 22 plants can be protected against attack by the abovementioned which are capable of restoring the male fertility in hybrid pathogens within a certain period of time after the treatment. plants that contain the genetic determinants responsible for The period of time within which protection is effected gen male-sterility. Genetic determinants for male sterility may be erally extends from 1 to 10 days, preferably 1 to 7 days, after located in the cytoplasm. Examples of cytoplasmic male Ste the treatment of the plants with the active compounds. rility (CMS) were for instance described in Brassica species. 0.174 Plants and plant cultivars which are also preferably However, genetic determinants for male sterility can also be to be treated according to the invention are resistant against located in the nuclear genome. Malesterile plants can also be one or more biotic stresses, i.e. said plants show a better obtained by plant biotechnology methods such as genetic defense against animal and microbial pests, such as against engineering. A particularly useful means of obtaining male nematodes, insects, mites, phytopathogenic fungi, bacteria, sterile plants is described in WO 89/10396 in which, for viruses and/or viroids. example, a ribonuclease Such as barnase is selectively 0175 Plants and plant cultivars which may also be treated expressed in the tapetum cells in the stamens. Fertility can according to the invention are those plants which are resistant then be restored by expression in the tapetum cells of a ribo to one or more abiotic stresses, i. e. that already exhibit an nuclease inhibitor Such as barstar. increased plant health with respect to stress tolerance. Abiotic 0.178 Plants or plant cultivars (obtained by plant biotech stress conditions may include, for example, drought, cold nology methods such as genetic engineering) which may be temperature exposure, heat exposure, osmotic stress, flood treated according to the invention are herbicide-tolerant ing, increased soil salinity, increased mineral exposure, oZon plants, i.e. plants made tolerant to one or more given herbi exposure, highlight exposure, limited availability of nitrogen cides. Such plants can be obtained either by genetic transfor nutrients, limited availability of phosphorus nutrients, shade mation, or by selection of plants containing a mutation avoidance. Preferably, the treatment of these plants and cul imparting Such herbicide tolerance. tivars with the composition of the present invention addition 0179 Herbicide-tolerant plants are for example glypho ally increases the overall plant health (cf. above). sate-tolerant plants, i.e. plants made tolerant to the herbicide 0176 Plants and plant cultivars which may also be treated glyphosate or salts thereof. Plants can be made tolerant to according to the invention, are those plants characterized by glyphosate through different means. For example, glypho enhanced yield characteristics, i. e. that already exhibit an sate-tolerant plants can be obtained by transforming the plant increased plant health with respect to this feature. Increased with a gene encoding the enzyme 5-enolpyruvylshikimate-3- yield in said plants can be the result of for example, improved phosphate synthase (EPSPS). Examples of such EPSPS plant physiology, growth and development. Such as water use genes are the AroA gene (mutant CT7) of the bacterium efficiency, water retention efficiency, improved nitrogen use, Salmonella typhimurium, the CP4 gene of the bacterium enhanced carbon assimilation, improved photosynthesis, Agrobacterium sp., the genes encoding a Petunia EPSPS, a increased germination efficiency and accelerated maturation. Tomato EPSPS, or an Eleusine EPSPS. It can also be a Yield can furthermore be affected by improved plant archi mutated EPSPS. Glyphosate-tolerant plants can also be tecture (under stress and non-stress conditions), including but obtained by expressing a gene that encodes a glyphosate not limited to, early flowering, flowering control for hybrid oxido-reductase enzyme. Glyphosate-tolerant plants can also seed production, seedling vigor, plant size, internode number be obtained by expressing a gene that encodes a glyphosate and distance, root growth, seed size, fruit size, pod size, pod acetyl transferase enzyme. Glyphosate-tolerant plants can or ear number, seed number per pod or ear, seed mass, also be obtained by selecting plants containing naturally enhanced seed filling, reduced seed dispersal, reduced pod occurring mutations of the above-mentioned genes. dehiscence and lodging resistance. Further yield traits 0180. Other herbicide resistant plants are for example include seed composition, Such as carbohydrate content, pro plants that are made tolerant to herbicides inhibiting the tein content, oil content and composition, nutritional value, enzyme glutamine synthase. Such as bialaphos, phosphino reduction in anti-nutritional compounds, improved process thricin or glufosinate. Such plants can be obtained by express ability and better storage stability. Preferably, the treatment of ing an enzyme detoxifying the herbicide or a mutant these plants and cultivars with the composition of the present glutamine synthase enzyme that is resistant to inhibition. One invention additionally increases the overall plant health (cf. Such efficient detoxifying enzyme is an enzyme encoding a above). phosphinothricin acetyltransferase (such as the bar or pat 0177 Plants that may be treated according to the invention protein from Streptomyces species). Plants expressing an are hybrid plants that already express the characteristic of exogenous phosphinothricin acetyltransferase are also heterosis or hybrid vigor which results in generally higher described. yield, vigor, health and resistance towards biotic and abiotic 0181 Further herbicide-tolerant plants are also plants that stress factors. Such plants are typically made by crossing an are made tolerant to the herbicides inhibiting the enzyme inbred male-sterile parent line (the female parent) with hydroxyphenylpyruvatedioxygenase (HPPD). Hydroxyphe another inbred male-fertile parent line (the male parent). nylpyruvatedioxygenases are enzymes that catalyze the reac Hybrid seed is typically harvested from the malesterile plants tion in which para-hydroxyphenylpyruvate (HPP) is trans and sold to growers. Malesterile plants can sometimes (e.g. in formed into homogentisate. Plants tolerant to HPPD corn) be produced by detasseling, i.e. the mechanical removal inhibitors can be transformed with a gene encoding a of the male reproductive organs (or males flowers) but, more naturally-occurring resistant HPPD enzyme, or a gene encod typically, malesterility is the result of genetic determinants in ing a mutated HPPD enzyme. Tolerance to HPPD-inhibitors the plant genome. In that case, and especially when seed is the can also be obtained by transforming plants with genes desired product to be harvested from the hybrid plants it is encoding certain enzymes enabling the formation of typically useful to ensure that male fertility in the hybrid homogentisate despite the inhibition of the native HPPD plants is fully restored. This can be accomplished by ensuring enzyme by the HPPD-inhibitor. Tolerance of plants to HPPD that the male parents have appropriate fertility restorer genes inhibitors can also be improved by transforming plants with a US 2015/O 141245 A1 May 21, 2015 gene encoding an enzyme prephenate dehydrogenase in addi second secreted protein from Bacillus thuringiensis or tion to a gene encoding an HPPD-tolerant enzyme. B. cereus, such as the binary toxin made up of the VIP1A 0182 Still further herbicide resistant plants are plants that and VIP2A proteins; or are made tolerant to acetolactate synthase (ALS) inhibitors. 0191 7) hybrid insecticidal protein comprising parts Known ALS-inhibitors include, for example, sulfonylurea, from different secreted proteins from Bacillus thuring imidazolinone, triazolopyrimidines, pyrimidinyoxy (thio) iensis or Bacillus cereus, such as a hybrid of the proteins benzoates, and/or Sulfonylaminocarbonyltriazolinone herbi in 1) above or a hybrid of the proteins in 2) above; or cides. Different mutations in the ALS enzyme (also known as 0.192 8) protein of any one of 1) to 3) above wherein acetohydroxyacid synthase, AHAS) are known to confer tol Some, particularly 1 to 10, amino acids have been erance to different herbicides and groups of herbicides. The replaced by anotheramino acid to obtain a higher insec production of Sulfonylurea-tolerant plants and imidazoli ticidal activity to a targetinsect species, and/or to expand none-tolerant plants is described in WO 1996/033270. Other the range of target insect species affected, and/or imidazolinone-tolerant plants are also described. Further sul because of changes introduced into the encoding DNA fonylurea- and imidazolinone-tolerant plants are also during cloning or transformation (while still encoding described in for example WO 2007/024782. an insecticidal protein), such as the VIP3Aa protein in 0183. Other plants tolerant to imidazolinone and/or sulfo cotton event COT 102. nylurea can be obtained by induced mutagenesis, selection in 0.193) Of course, an insect-resistant transgenic plant, as cell cultures in the presence of the herbicide or mutation used herein, also includes any plant comprising a combina breeding as described for example for soybeans, for rice, for tion of genes encoding the proteins of any one of the above sugar beet, for lettuce, or for sunflower. classes 1 to 8. In one embodiment, an insect-resistant plant 0184 Plants or plant cultivars (obtained by plant biotech contains more than one transgene encoding a protein of any nology methods such as genetic engineering) which may also one of the above classes 1 to 8, to expand the range of target be treated according to the invention are insect-resistant insect species affected when using different proteins directed transgenic plants, i.e. plants made resistant to attack by cer at different target insect species, or to delay insect resistance tain target insects. Such plants can be obtained by genetic development to the plants by using different proteins insecti transformation, or by selection of plants containing a muta cidal to the same target insect species but having a different tion imparting Such insect resistance. mode of action, such as binding to different receptor binding 0185. An “insect-resistant transgenic plant, as used sites in the insect. herein, includes any plant containing at least one transgene (0194 Plants or plant cultivars (obtained by plant biotech comprising a coding sequence encoding: nology methods such as genetic engineering) which may also 0186 1) An insecticidal crystal protein from Bacillus thu be treated according to the invention are tolerant to abiotic ringiensis oran insecticidal portion thereof. Such as the insec stresses. Such plants can be obtained by genetic transforma ticidal crystal proteins listed online at: http://www.lifesci. tion, or by selection of plants containing a mutation imparting Sussex.ac.uk/Home/Neil Crickmore/Bt/, or insecticidal portions thereof, e.g., proteins of the Cry protein classes Cry1 Such stress resistance. Particularly useful stress tolerance Ab, Cry1Ac, Cry1 F, Cry2Ab, Cry3Aa, or Cry3Bb or insec plants include: ticidal portions thereof; or 0.195 a. plants which contain a transgene capable of 0187. 2) a crystal protein from Bacillus thuringiensis or reducing the expression and/or the activity of poly a portion thereof which is insecticidal in the presence of (ADP-ribose)polymerase (PARP) gene in the plant cells a second other crystal protein from Bacillus thuringien or plants sis or a portion thereof. Such as the binary toxin made up 0.196 b. plants which contain a stress tolerance enhanc of the Cry34 and Cry35 crystal proteins; or ing transgene capable of reducing the expression and/or 0188 3) a hybrid insecticidal protein comprising parts the activity of the poly(ADP-ribose)glycohydrolase of different insecticidal crystal proteins from Bacillus (PARG) encoding genes of the plants or plants cells. thuringiensis, such as a hybrid of the proteins of 1) above 0.197 c. plants which contain a stress tolerance enhanc or a hybrid of the proteins of 2) above, e.g., the Cry1 ing transgene coding for a plant-functional enzyme of A.105 protein produced by cornevent MON98034 (WO the nicotinamide adenine dinucleotide Salvage synthesis 2007/027777); or pathway including nicotinamidase, nicotinate phospho 0189 4) a protein of any one of 1) to 3) above wherein ribosyltransferase, nicotinic acid mononucleotide ade Some, particularly 1 to 10, amino acids have been nyl transferase, nicotinamide adenine dinucleotide syn replaced by another amino acid to obtain a higher insec thetase or nicotine amide phosphorybosyltransferase. ticidal activity to a target insect species, and/or to expand 0198 Plants or plant cultivars (obtained by plant biotech the range of target insect species affected, and/or nology methods such as genetic engineering) which may also because of changes introduced into the encoding DNA be treated according to the invention show altered quantity, during cloning or transformation, Such as the Cry3Bb1 quality and/or storage-stability of the harvested product and/ protein in corn events MON863 or MON88017, or the or altered properties of specific ingredients of the harvested Cry3A protein in corn event MIR604; 5) an insecticidal product Such as: secreted protein from Bacillus thuringiensis or Bacillus 0199. 1) transgenic plants which synthesize a modified cereus, or an insecticidal portion thereof. Such as the starch, which in its physical-chemical characteristics, in vegetative insecticidal (VIP) proteins listed at: http:// particular the amylose content or the amylose/amy www.lifesci. Sussex.ac.uk/home/Neil Crickmore/Bt/ lopectin ratio, the degree of branching, the average chain Vip.html, e.g. proteins from the VIP3Aa protein class; or length, the side chain distribution, the viscosity behav 0190. 6) secreted protein from Bacillus thuringiensis or iour, the gelling strength, the starch grain size and/or the Bacillus cereus which is insecticidal in the presence of a starch grain morphology, is changed in comparison with US 2015/O 141245 A1 May 21, 2015 24

the synthesised Starch in wild type plant cells or plants, nylureas, for example maize). Herbicide-resistant plants So that this is better Suited for special applications. (plants bred in a conventional manner for herbicide tolerance) 0200 2) transgenic plants which synthesize non starch which may be mentioned include the varieties sold under the carbohydrate polymers or which synthesize non starch name Clearfield(R) (for example maize). carbohydrate polymers with altered properties in com parison to wildtype plants without genetic modification. 0214 Particularly useful transgenic plants which may be Examples are plants producing polyfructose, especially treated according to the invention are plants containing trans of the inulin and levan-type, plants producing alpha 1.4 formation events, or a combination of transformation events, glucans, plants producing alpha-1,6 branched alpha-1, and that are listed for example in the databases for various 4-glucans, plants producing alternan, national or regional regulatory agencies including Event 0201 3) transgenic plants which produce hyaluronan. 1143-14A (cotton, insect control, not deposited, described in 0202 Plants or plant cultivars (that can be obtained by WO 06/128569); Event 1143-51B (cotton, insect control, not plant biotechnology methods such as genetic engineering) deposited, described in WO 06/128570): Event 1445 (cotton, which may also be treated according to the invention are herbicide tolerance, not deposited, described in US-A 2002 plants, such as cotton plants, with altered fiber characteristics. 120964 or WO 02/034946); Event 17053 (rice, herbicide Such plants can be obtained by genetic transformation or by tolerance, deposited as PTA-9843, described in WO selection of plants contain a mutation imparting Such altered 10/117737); Event 17314 (rice, herbicide tolerance, depos fiber characteristics and include: ited as PTA-9844, described in WO 10/117735): Event 281 24-236 (cotton, insect control-herbicide tolerance, deposited 0203 a) Plants, such as cotton plants, containing an as PTA-6233, described in WO 05/103266 or US-A 2005 altered form of cellulose synthase genes, 216969): Event 3006-210-23 (cotton, insect control-herbi 0204 b) Plants, such as cotton plants, containing an cide tolerance, deposited as PTA-6233, described in US-A altered form of rSw2 or rSW3 homologous nucleic acids, 2007-143876 or WO 05/103266): Event 3272 (corn, quality 0205 c) Plants, such as cotton plants, with increased trait, deposited as PTA-9972, described in WO 06/098952 or expression of Sucrose phosphate synthase, US-A 2006-230473); Event 40416 (corn, insect control-her 0206 d) Plants, such as cotton plants, with increased bicide tolerance, deposited as ATCC PTA-11508, described expression of Sucrose synthase, in WO 11/075593); Event 43A47 (corn, insect control-herbi 0207 e) Plants, such as cotton plants, wherein the tim cide tolerance, deposited as ATCC PTA-11509, described in ing of the plasmodesmatal gating at the basis of the fiber WO 11/075595): Event 5307 (corn, insect control, deposited cell is altered, e.g. through downregulation of fiberse as ATCC PTA-9561, described in WO 10/077816); Event lective f31.3-glucanase, ASR-368 (bent grass, herbicide tolerance, deposited as ATCC 0208 f) Plants, such as cotton plants, having fibers with PTA-4816, described in US-A 2006-162007 or WO altered reactivity, e.g. through the expression of 04/053062): Event B16 (corn, herbicide tolerance, not depos N-acteylglucosaminetransferase gene including nodC ited, described in US-A 2003-126634); Event BPS-CV127-9 and chitinsynthase genes. (soybean, herbicide tolerance, deposited as NCIMB No. 0209 Plants or plant cultivars (that can be obtained by 41603, described in WO 10/080829): Event CE43-67B (cot plant biotechnology methods such as genetic engineering) ton, insect control, deposited as DSMACC2724, described in which may also be treated according to the invention are US-A 2009-217423 or WO 06/128573); Event CE44-69D plants, such as oilseed rape or related Brassica plants, with (cotton, insect control, not deposited, described in US-A altered oil profile characteristics. Such plants can be obtained 2010-0024077); Event CE44-69D (cotton, insect control, not by genetic transformation or by selection of plants contain a deposited, described in WO 06/128571): Event CE46-02A mutation imparting Such altered oil characteristics and (cotton, insect control, not deposited, described in WO include: 06/128572); Event COT102 (cotton, insect control, not 0210 a) Plants, such as oilseed rape plants, producing deposited, described in US-A 2006-130175 or WO oil having a high oleic acid content, 04/039986); Event COT202 (cotton, insect control, not 0211 b) Plants such as oilseed rape plants, producing deposited, described in US-A 2007-067868 or WO oil having a low linolenic acid content, 05/054479); Event COT203 (cotton, insect control, not 0212 c) Plant such as oilseed rape plants, producing oil deposited, described in WO 05/054480); Event DAS40278 having a low level of Saturated fatty acids. (corn, herbicide tolerance, deposited as ATCC PTA-10244, 0213 Particularly useful transgenic plants which may be described in WO 11/022469): Event DAS-59122-7 (corn, treated according to the invention are plants which comprise insect control-herbicide tolerance, deposited as ATCC PTA one or more genes which encode one or more toxins, such as 11384, described in US-A 2006-070139): Event DAS-59132 the following which are sold under the trade names YIELD (corn, insect control-herbicide tolerance, not deposited, GARDR) (for example maize, cotton, soya beans), Knock described in WO 09/100188); Event DAS68416 (soybean, Out(R) (for example maize), BiteGard(R) (for example maize), herbicide tolerance, deposited as ATCC PTA-10442. BtXtra.R. (for example maize), StarLink R. (for example described in WO 11/066384 or WO 11/066360): Event maize), Bollgard(R) (cotton), Nucotnir (cotton), Nucotn DP-098140-6 (corn, herbicide tolerance, deposited as ATCC 33B(R) (cotton), NatureGardr (for example maize), Pro PTA-8296, described in US-A 2009-137395 or WO tectaR) and NewLeafR (potato). Examples of herbicide-tol 08/112019); Event DP-305423-1 (soybean, quality trait, not erant plants which may be mentioned are maize varieties, deposited, described in US-A 2008-312082 or WO cotton varieties and Soya bean varieties which are sold under 08/054747); Event DP-32138-1 (corn, hybridization system, the trade names Roundup Ready R (tolerance to glyphosate, deposited as ATCC PTA-9158, described in US-A 2009 for example maize, cotton, Soya bean), Liberty Link. R (toler 0210970 or WO 09/103049): Event DP-356043-5 (soybean, ance to phosphinotricin, for example oilseed rape), IMIR) herbicide tolerance, deposited as ATCCPTA-8287, described (tolerance to imidazolinones) and STSR) (tolerance to sulpho in US-A 2010-0184079 or WO 08/002872); Event EE-1 US 2015/O 141245 A1 May 21, 2015

(brinjal, insect control, not deposited, described in WO (soybean, herbicide tolerance, deposited as ATCCPTA-6708, 07/091277); Event FI117 (corn, herbicide tolerance, depos described in US-A 2006-282915 or WO 06/130436): Event ited as ATCC 209031, described in US-A 2006-059581 or MS11 (oilseed rape, pollination control-herbicide tolerance, WO 98/044140); Event GA21 (corn, herbicide tolerance, deposited as ATCC PTA-850 or PTA-2485, described in WO deposited as ATCC 209033, described in US-A 2005-086719 01/031042); Event MS8 (oilseed rape, pollination control or WO98/044140); Event GG25 (corn, herbicide tolerance, herbicide tolerance, deposited as ATCC PTA-730, described deposited as ATCC 209032, described in US-A 2005-188434 in WO 01/041558 or US-A 2003-188347); Event NK603 or WO98/044140); Event GHB119 (cotton, insect control (corn, herbicide tolerance, deposited as ATCC PTA-2478, herbicide tolerance, deposited as ATCCPTA-8398, described described in US-A 2007-292.854); Event PE-7 (rice, insect in WO 08/151780); Event GHB614 (cotton, herbicide toler control, not deposited, described in WO 08/114282); Event ance, deposited as ATCC PTA-6878, described in US-A RF3 (oilseed rape, pollination control-herbicide tolerance, 2010-050282 or WO 07/017186); Event GJ11 (corn, herbi deposited as ATCC PTA-730, described in WO 01/041558 or cide tolerance, deposited as ATCC 209030, described in US-A 2003-188347); Event RT73 (oilseed rape, herbicide US-A 2005-188434 or WO 98/044140); Event GM RZ13 tolerance, not deposited, described in WO 02/036831 or (sugar beet, virus resistance, deposited as NCIMB-41601, US-A 2008-070260): Event T227-1 (sugar beet, herbicide described in WO 10/076212); Event H7-1 (sugar beet, herbi tolerance, not deposited, described in WO 02/44407 or US-A cide tolerance, deposited as NCIMB 41158 or NCIMB 2009-265817); Event T25 (corn, herbicide tolerance, not 41159, described in US-A 2004-172669 or WO 04/074492); deposited, described in US-A 2001-029014 or WO Event JOPLIN1 (wheat, disease tolerance, not deposited, 01/051654); Event T304-40 (cotton, insect control-herbicide described in US-A2008-064032); Event LL27 (soybean, her tolerance, deposited as ATCC PTA-8171, described in US-A bicide tolerance, deposited as NCIMB41658, described in 2010-077501 or WO 08/122406); Event T342-142 (cotton, WO 06/108674 or US-A 2008-320616); Event LL55 (soy insect control, not deposited, described in WO 06/128568); bean, herbicide tolerance, deposited as NCIMB 41660, Event TC1507 (corn, insect control-herbicide tolerance, not described in WO 06/108675 or US-A 2008-196127): Event deposited, described in US-A 2005-039226 or WO LLcotton25 (cotton, herbicide tolerance, deposited as ATCC 04/099447); Event VIP1034 (corn, insect control-herbicide PTA-3343, described in WO 03/013224 or US-A 2003 tolerance, deposited as ATCC PTA-3925, described in WO 097687); Event LLRICE06 (rice, herbicide tolerance, depos 03/052073), Event 32316 (corn, insect control-herbicide tol ited as ATCC-23352, described in U.S. Pat. No. 6,468,747 or erance, deposited as PTA-11507, described in WO WO 00/026345): Event LLRICE601 (rice, herbicide toler 11/084632), Event 4114 (corn, insect control-herbicide tol ance, deposited as ATCC PTA-2600, described in US-A erance, deposited as PTA-11506, described in WO 2008-2289060 or WO 00/026356); Event LYO38 (corn, qual 11/084621). ity trait, deposited as ATCC PTA-5623, described in US-A 0215 Particularly useful transgenic plants which may be 2007-028322 or WO 05/061720): Event MIR162 (corn, treated according to the invention are plants containing trans insect control, deposited as PTA-8166, described in US-A formation events, or combination of transformation events, 2009-300784 or WO 07/142840); Event MIR604 (corn, that are listed for example in the databases from various insect control, not deposited, described in US-A 2008 national or regional regulatory agencies (see for example 167456 or WO 05/103301); Event MON15985 (cotton, insect http://gmoinfo.jrc.it/gmp browse.aspx and http://www.ag control, deposited as ATCC PTA-2516, described in US-A bios.com/dbase.php). 2004-250317 or WO 02/100163): Event MON810 (corn, insect control, not deposited, described in US-A 2002 102582); Event MON863 (corn, insect control, deposited as EXAMPLES ATCC PTA-2605, described in WO 04/011601 or US-A 2006-095986); Event MON87427 (corn, pollination control, 0216. The advanced fungicidal activity of the active com deposited as ATCC PTA-7899, described in WO 117062904); pound combinations according to the invention is evident Event MON87460 (corn, stress tolerance, deposited as ATCC from the example below. While the individual active com PTA-8910, described in WO 09/11 1263 or US-A 2011 pounds exhibit weaknesses with regard to the fungicidal 0138504); Event MON87701 (soybean, insect control, activity, the combinations have an activity which exceeds a deposited as ATCC PTA-8194, described in US-A 2009 simple addition of activities. 130071 or WO 09/064652); Event MON87705 (soybean, 0217. A synergistic effect of fungicides is always present quality trait herbicide tolerance, deposited as ATCC PTA when the fungicidal activity of the active compound combi 9241, described in US-A 2010-0080887 or WO 10/037016); nations exceeds the total of the activities of the active com Event MON87708 (soybean, herbicide tolerance, deposited pounds when applied individually. The expected activity for a as ATCC PTA9670, described in WO 11/034704); Event given combination of two active compounds can be calcu MON87754 (soybean, quality trait, deposited as ATCC PTA lated as follows (according to Colby's formula) (cf. Colby, 9385, described in WO 10/024976); Event MON87769 (soy S.R., "Calculating Synergistic and Antagonistic Responses of bean, quality trait, deposited as ATCC PTA-8911, described Herbicide Combinations, Weeds 1967, 15, 20-22): in US-A 2011-0067141 or WO 09/102873); Event MON88017 (corn, insect control-herbicide tolerance, depos ited as ATCC PTA-5582, described in US-A 2008-0284.82 or If WO 05/059103): Event MON88913 (cotton, herbicide toler 0218. X is the efficacy when active compound A is applied ance, deposited as ATCC PTA-4854, described in WO at an application rate of m ppm (org/ha), 04/072235 or US-A2006-059590); Event MON89034 (corn, insect control, deposited as ATCC PTA-7455, described in 0219 Y is the efficacy when active compound B is applied WO 07/140256 or US-A 2008-260932); Event MON89788 at an application rate of n ppm (org/ha), US 2015/O 141245 A1 May 21, 2015 26

0220 E is the efficacy when the active compounds A and B TABLE A are applied at application rates of m and n ppm (org/ha), respectively, and Phytophthora test (tomatoes), preventive then Application rate of active compound Efficacy in 90 Active compounds in ppm a.i. found calc.** E = x + Y-8r - B19 QST30002 500 12 F71 flutolani 1OO 33 B19 - F71 1:0.2 SOO - 100 70 41 0221) The degree of efficacy, expressed in % is denoted. *found = activity found 0% means an efficacy which corresponds to that of the control ** calc. = activity calculated using Colby's formula while an efficacy of 100% means that no disease is observed. 0222. If the actual fungicidal activity exceeds the calcu Example B lated value, then the activity of the combination is superad ditive, i.e. a synergistic effect exists. In this case, the efficacy Venturia Test (Apples)/Preventive which was actually observed must be greater than the value for the expected efficacy (E) calculated from the abovemen 0231 QST30002 (B19), active compunds (1 part by tioned formula. weight) solved in acetone/dimethylacetamide (24.5/24.5 part 0223) A further way of demonstrating a synergistic effect by weight) and alkylaryl polyglycol ether (1 part by weight), is the method of Tammes (cf. 'Isoboles, a graphic represen or combinations thereof were diluted with water to the desired tation of synergism in pesticides’ in Neth, J. Plant Path. concentration. 1964, 70, 73-80). 0232. The application rate of QST30002 refers to the 0224. The invention is illustrated by the following amount of dried Bacillus subtilis AQ30002 aka QST300202 examples. However the invention is not limited to the (NRRL Accession No. B-50421. examples. 0233. To test for preventive activity, young plants are sprayed with the preparation of active compound at the stated 0225. In the following examples the strain Bacillus subti rate of application. After the spray coating has dried on, the lis AQ30002 has been used, which is mentioned above as B19 plants are inoculated with an aqueous conidia Suspension of and is referred to in the following table as QST3002. A solu the causal agent of apple scab (Venturia inaequalis) and then tion comprising 8.510 CFU/g (1.34%) of this strain has been remain for 1 day in an incubation cabinet at approximately used. 20° C. and a relative atmospheric humidity of 100%. 0234. The plants are then placed in a greenhouse at Example a approximately 21°C. and a relative atmospheric humidity of approximately 90%. Phytophthora Test (Tomatoes)/Preventive 0235. The test is evaluated 10 days after the inoculation. 0226 QST30002 (B19), active compunds (1 part by 0% means an efficacy which corresponds to that of the weight) solved in acetone/dimethylacetamide (24.5/24.5 part untreated control, while an efficacy of 100% means that no by weight) and alkylaryl polyglycol ether (1 part by weight), disease is observed. or combinations thereof were diluted with water to the desired 0236. The table below clearly shows that the observed concentration. activity of the active compound combination according to the invention is greater than the calculated activity, i.e. a syner 0227. The application rate of QST30002 refers to the gistic effect is present. amount of dried Bacillus subtilis AQ30002 aka QST300202 (NRRL Accession No. B-50421. TABLE B1 0228 To test for preventive activity, young plants are sprayed with the preparation of active compound at the stated enturia test (apples) preventive rate of application. After the spray coating has dried on, the Application rate of plants are inoculated with an aqueous spore Suspension of active compound Efficacy in 90 Phytophthora infestans. The plants are then placed in an incubation cabinet at approximately 20° C. and a relative Active compounds in ppm a.i. found calc.** atmospheric humidity of 100%. B19 QST30002 500 33 250 O 0229. The test is evaluated 3 days after the inoculation.0% F70 fluopyram 4 4 means an efficacy which corresponds to that of the untreated F72 fluxapyroxad O.S 43 F84 penflufen 2 O control, while an efficacy of 100% means that no disease is B19 - F70 1:0.008 SOO - 4 83 36 observed. B19 - F721:O.OO2 2SO - O.S 97 43 0230. The table below clearly shows that the observed B19 - F84 1:0.004 SOO - 2 99 33 activity of the active compound combination according to the *found = activity found invention is greater than the calculated activity, i.e. a syner ** calc. = activity calculated using Colby's formula gistic effect is present. US 2015/O 141245 A1 May 21, 2015 27

TABLE B2 TABLE C2-continued enturia test (apples), preventive Sphaerotheca test (cucumbers) preventive Application rate of Application rate of active compound Efficacy in 90 active compound Efficacy in % Active compounds in ppm a.i. found calc.** Active compounds in ppm a.i. found calc.** B19 QST30002 500 63 F71 flutolani 1OO 4 F72 fluxapyroxad 1 29 B19 - F71 1:0.2 SOO + 100 74 64 F84 penflufen 2 O B19 - F70 1:0.008 SOO - 4 79 36 *found = activity found B19 - F721:O.OO2 500 - 1 93 50 **calc. = activity calculated using Colby's formula B19 - F84 1:0.004 SOO - 2 52 29

*found = activity found Example C ** calc. = activity calculated using Colby's formula Sphaerotheca Test (Cucumbers)/Preventive 0237 QST30002 (B19), active compunds (1 part by Example D weight) solved in acetone/dimethylacetamide (24.5/24.5 part by weight) and alkylaryl polyglycol ether (1 part by weight), or combinations thereof were diluted with water to the desired Alternaria Test (Tomatoes)/Preventive concentration. 0238. The application rate of QST30002 refers to the 0242 QST30002 (B19), active compunds (1 part by amount of dried Bacillus subtilis AQ30002 (NRRL Acces weight) solved in acetone/dimethylacetamide (24.5/24.5 part sion No. B-50421), contained in the product QST30002. by weight) and alkylaryl polyglycol ether (1 part by weight), 0239. To test for preventive activity, young plants are or combinations thereof were diluted with water to the desired sprayed with the preparation of active compound at the stated concentration. rate of application. After the spray coating has dried on, the plants are inoculated with an aqueous spore suspension of 0243 The application rate of QST30002 refers to the Sphaerotheca fuliginea. The plants are then placed in agreen amount of dried Bacillus subtilis AQ30002 aka QST300202 house at approximately 23° C. and a relative atmospheric (NRRL Accession No. B-50421. humidity of approximately 70%. 0244. To test for preventive activity, young plants are 0240. The test is evaluated 7 days after the inoculation.0% sprayed with the preparation of active compound at the stated means an efficacy which corresponds to that of the untreated control, while an efficacy of 100% means that no disease is rate of application. After the spray coating has dried on, the observed. plants are inoculated with an aqueous spore Suspension of 0241 The table below clearly shows that the observed Alternaria Solani. The plants are then placed in an incubation activity of the active compound combination according to the cabinet at approximately 20° C. and a relative atmospheric invention is greater than the calculated activity, i.e. a syner humidity of 100%. gistic effect is present. 0245. The test is evaluated 3 days after the inoculation.0% means an efficacy which corresponds to that of the untreated TABLE C1 control while an efficacy of 100% means that no disease is Sphaerotheca test (cucumbers) preventive observed. Application rate of 0246 The table below clearly shows that the observed active compound Efficacy in 90 activity of the active compound combination according to the invention is greater than the calculated activity, i.e. a syner Active compounds in ppm a.i. found calc.** gistic effect is present. B19 QST30002 500 O F71 flutolani 1OO O TABLE D1 B19 - F71 1:0.2 SOO + 100 62 O *found = activity found Alternaria test (tomatoes), preventive **calc. = activity calculated using Colby's formula Application rate of active compound Efficacy in 90 TABLE C2 Active compounds in ppm a.i. found calc.** Sphaerotheca test (cucumbers) preventive B19 QST30002 500 52 250 34 Application rate of F70 fluopyram 4 33 active compound Efficacy in 90 F72 fluxapyroxad 1 47 B19 - F70 1:0.008 SOO - 4 82 68 Active compounds in ppm a.i. found calc.** B19 - F721:O.OO2 500 - 1 81 75 B19 QST30002 500 29 *found = activity found F70 fluopyram 4 10 ** calc. = activity calculated using Colby's formula US 2015/O 141245 A1 May 21, 2015 28

TABLE D2 55614), Bacillus thuringiensis BD#32 (NRRL Accession No. B-21530), Bacillus thuringiensis AQ52 (NRRL Accession Alternaria test (tomatoes), preventive No. B-21619), Muscodor albus 620 (NRRL Accession No. Application rate of 30547), Muscodor roseus A3-5 (NRRL Accession No. active compound Efficacy in 90 30548), Rhodococcus globerulus AQ719 (NRRL Accession No. B-21663), Streptomyces galbus (NRRL Accession No. Active compounds in ppm a.i. found calc.** 30232), Streptomyces sp. (NRRL Accession No. B-301.45), B19 QST30002 250 32 Bacillus thuringiensis subspec. Kurstaki BMP 123, Bacillus F71 flutolani 50 43 subtilis AQ30002 (NRRL Accession No. B-50421), and B19 - F71 1:0.2 2SO - SO 91 61 Bacillus subtilis AQ30004 (NRRL Accession No. B-50455), *found = activity found and/or a mutant of these strains having all identifying char **calc. = activity calculated using Colby's formula acteristics of the respective strain, and/or a metabolite pro duced by the respective strain that exhibits activity against insects, mites, nematodes and/or phytopathogens and at least Example E one fungicide (I) selected from the group consisting of inhibi tors of the respiratory chain at complex I or II in a synergis Botrytis Test (Beans)/Preventive tically effective amount. 0247 QST30002 (B19), active compunds (1 part by 2. The composition according to claim 1, wherein the weight) solved in acetone/dimethylacetamide (24.5/24.5 part inhibitor of the respiratory chain at complex I or II is selected by weight) and alkylaryl polyglycol ether (1 part by weight), from the group consisting of biXafen, boscalid, carboxin, or combinations thereof were diluted with water to the desired diflumetorim, fenfuram, fluopyram, flutolanil, fluxapyroxad, concentration. furametpyr, furmecyclox, isopyrazam (mixture of Syn 0248. The application rate of QST30002 refers to the epimeric racemate 1RS4SR.9RS and anti-epimeric racemate amount of dried Bacillus subtilis AQ30002 aka QST300202 1RS.4SR.9SR), isopyrazam (anti-epimeric racemate 1RS, (NRRL Accession No. B-50421. 4SR.9SR), isopyrazam (anti-epimeric enantiomer 1 R,4S.9S), 0249. To test for preventive activity, young plants are isopyrazam (anti-epimeric enantiomer 1S.4R.9R), isopy sprayed with the preparation of active compound. After the razam (syn epimeric racemate 1RS4SR,9RS), isopyrazam spray coating has dried on, 2 small pieces of agar covered (Syn-epimeric enantiomer 1 R,4S,9R), isopyrazam (Syn with growth of Botrytis cinerea are placed on each leaf. The epimeric enantiomer 1S,4R.9S), mepronil), oxycarboxin, inoculated plants are placed in a darkened chamber at 20°C. penflufen, penthiopyriad, sedaxane, thifluZamide, 1-methyl and a relative atmospheric humidity of 100%. N-2-(1,1,2,2-tetrafluoroethoxy)phenyl-3-(trifluorom 0250 2 days after the inoculation, the size of the lesions on ethyl)-1H-pyrazole-4-carboxamide, 3-(difluoromethyl)-1- the leaves is evaluated. 0% means an efficacy which corre methyl-N-2-(1,1,2,2-tetrafluoroethoxy)phenyl)-1H sponds to that of the untreated control, while an efficacy of pyrazole-4-carboxamide, 3-(difluoromethyl)-N-4-fluoro-2- 100% means that no disease is observed. (1,1,2,3,3,3-hexafluoropropoxy)phenyl-1-methyl-1H 0251. The table below clearly shows that the observed pyrazole-4-carboxamide, N-1-(2,4-dichlorophenyl)-1- activity of the active compound combination according to the methoxypropan-2-yl)-3-(difluoromethyl)-1-methyl-1H invention is greater than the calculated activity, i.e. a syner pyrazole-4-carboxamide, 5,8-difluoro-N-2-(2-fluoro-4-4- gistic effect is present. (trifluoromethyl)pyridin-2-yl)oxyphenyl)ethylquinazolin 4-amine, benzovindiflupyr. N-(1S,4R)-9- (dichloromethylene)-1,2,3,4-tetrahydro-1,4- TABLE E methanonaphthalen-5-yl)-3-(difluoromethyl)-1-methyl-1H Asotrytis test (beans) preventive pyrazole-4-carboxamide, N-(1R,4S)-9- (dichloromethylene)-1,2,3,4-tetrahydro-1,4- Application rate of methanonaphthalen-5-yl)-3-(difluoromethyl)-1-methyl-1H active compound Efficacy in 90 pyrazole-4-carboxamide, 3-(Difluorimethyl)-1-methyl-N-(1, Active compounds in ppm a.i. found calc.** 1,3-trimethyl-2,3-dihydro-1H-inden-4-yl)-1H-pyrazol-4- carboxamid, 1,3,5-Trimethyl-N-(1,1,3-trimethyl-2,3- B19 QST30002 500 O F71 flutolani 1OO O dihydro-1H-inden-4-yl)-1H-pyrazol-4-carboxamid, B19 - F71 1:0.2 SOO + 100 43 O 1-Methyl-3-(trifluorimethyl)-N-(1,3,3-trimethyl-2,3-dihy dro-1H-inden-4-yl)-1H-pyrazol-4-carboxamid, 1-Methyl-3- *found = activity found (trifluormethyl)-N-(1S)-1,3,3-trimethyl-2,3-dihydro-1H **calc. = activity calculated using Colby's formula inden-4-yl)-1H-pyrazol-4-carboxamid, 1-Methyl-3- 1. A composition comprising at least one biological control (trifluormethyl)-N-(1R)-1,3,3-trimethyl-2,3-dihydro-1H agent selected from the group consisting of Bacillus chitino inden-4-yl)-1H-pyrazol-4-carboxamid, 3-(Difluorimethyl)- sporus AQ746 (NRRL Accession No. B-21618), Bacillus 1-methyl-N-(3S)-1,1,3-trimethyl-2,3-dihydro-1H-inden-4- mycoides AQ726 (NRRL Accession No. B-21664), Bacillus yl)-1H-pyrazol-4-carboxamid, 3-(Difluorimethyl)-1-methyl pumilus (NRRL Accession No. B-30087), Bacillus pumilus N-(3R)-1,1,3-trimethyl-2,3-dihydro-1H-inden-4-yl)-1H AQ717 (NRRL Accession No. B-21662), Bacillus sp. AQ175 pyrazol-4-carboxamid, 1,3,5-Trimethyl-N-(3R)-1,1,3- (ATCC Accession No. 55608), Bacillus sp. AQ177 (ATCC trimethyl-2,3-dihydro-1H-inden-4-yl)-1H-pyrazol-4- Accession No. 55609), Bacillus sp. AQ178 (ATCC Accession carboxamid, 1,3,5-Trimethyl-N-(3S)-1,1,3-trimethyl-2,3- No. 53522), Bacillus subtilis AQ743 (NRRL Accession No. dihydro-1H-inden-4-yl)-1H-pyrazol-4-carboxamid. B-21665), Bacillus subtilis AQ713 (NRRL Accession No. 3. The composition according to claim 2, wherein fungi B-21661), Bacillus subtilis AQ153 (ATCC Accession No. cide (I) is bixafen, boscalid, carboxin, fluopyram, flutolanil,

US 2015/O 141245 A1 May 21, 2015 30

N-(1S)-1,2,3,4-tetrahydronaphthalen-1-yl-1,3-thiazole-4- 12. The composition according to claim 10 capable of carboxamide, pentyl (6-({(1-methyl-1H-tetrazol-5-yl) being used for treating conventional or transgenic plants or (phenyl)methylidenelamino oxy)methylpyridin-2- seed thereof. y1}carbamate, phenazine-1-carboxylic acid, quinolin-8-ol 13. A method for reducing overall damage of plants and plant parts as well as losses in harvested fruits or vegetables (134-31-6), quinolin-8-ol sulfate (2:1), tert-butyl 6-({(1- caused by insects, mites, nematodes and/or phytopathogens methyl-1H-tetrazol-5-yl)(phenyl)methylenelaminooxy) comprising simultaneously or sequentially applying at least methylpyridin-2-yl)carbamate, 1-methyl-3-(trifluorom one biological control agent selected from the group consist ethyl)-N-2'-(trifluoromethyl)biphenyl-2-yl)-1H-pyrazole ing of Bacillus chitinosporus AQ746 (NRRL Accession No. 4-carboxamide, N-(4-chlorobiphenyl-2-yl)-3- B-21618), Bacillus mycoides AQ726 (NRRL Accession No. (difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide, B-21664), Bacillus pumilus (NRRL Accession No. N-(2',4'-dichlorobiphenyl-2-yl)-3-(difluoromethyl)-1-me B-30087), Bacillus pumilus AQ717 (NRRL Accession No. thyl-1H-pyrazole-4-carboxamide, 3-(difluoromethyl)-1-me B-21662), Bacillus sp. AQ175 (ATCC Accession No. 55608), thyl-N-4'-(trifluoromethyl)biphenyl-2-yl)-1H-pyrazole-4- Bacillus sp. AQ177 (ATCC Accession No. 55609), Bacillus carboxamide, N-(2,5'-difluorobiphenyl-2-yl)-1-methyl-3- sp. AQ178 (ATCC Accession No. 53522), Bacillus subtilis AQ743 (NRRL Accession No. B-21665), Bacillus subtilis (trifluoromethyl)-1H-pyrazole-4-carboxamide, AQ713 (NRRL Accession No. B-21661), Bacillus subtilis 3-(difluoromethyl)-1-methyl-N-4'-(prop-1-yn-1-yl)biphe AQ153 (ATCC Accession No. 55614), Bacillus thuringiensis nyl-2-yl)-1H-pyrazole-4-carboxamide, 5-fluoro-1,3-dim BDi32 (NRRL Accession No. B-21530), Bacillus thuring ethyl-N-4'-(prop-1-yn-1-yl)biphenyl-2-yl)-1H-pyrazole-4- iensis AQ52 (NRRL Accession No. B-21619), Muscodor carboxamide, 2-chloro-N-4'-(prop-1-yn-1-yl)biphenyl-2- albus 620 (NRRL Accession No. 30547), Muscodor roseus ylpyridine-3-carboxamide, 3-(difluoromethyl)-N-4'-(3.3- A3-5 (NRRL Accession No. 30548), Rhodococcus globeru dimethylbut-1-yn-1-yl)biphenyl-2-yl)-1-methyl-1H lus AQ719 (NRRL Accession No. B-21663), Streptomyces pyrazole-4-carboxamide, N-4'-(3.3-dimethylbut-1-yn-1-yl) gallbus (NRRL Accession No. 30232), Streptomyces sp. biphenyl-2-yl)-5-fluoro-1,3-dimethyl-1H-pyrazole-4- (NRRL Accession No. B-30.145), Bacillus thuringiensis sub spec. kurstaki BMP 123, Bacillus subtilis AQ30002 (NRRL carboxamide, 3-(difluoromethyl)-N-(4-ethynylbiphenyl-2- Accession No. B-50421), and Bacillus subtilis AQ 30004 yl)-1-methyl-1H-pyrazole-4-carboxamide, N-(4- (NRRL Accession No. B-50455), and/or a mutant of these ethynylbiphenyl-2-yl)-5-fluoro-1,3-dimethyl-1H-pyrazole stains having all identifying characteristics of the respective 4-carboxamide, 2-chloro-N-(4-ethynylbiphenyl-2-yl) strain, and/or a metabolite produced by the respective strain pyridine-3-carboxamide, 2-chloro-N-4'-(3.3-dimethylbut that exhibits activity against insects, mites, nematodes and/or 1-yn-1-yl)biphenyl-2-ylpyridine-3-carboxamide, phytopathogens and at least one fungicide (I) selected from 4-(difluoromethyl)-2-methyl-N-4'-(trifluoromethyl)biphe the group consisting or inhibitors of the respiratory chain at nyl-2-yl-1,3-thiazole-5-carboxamide, 5-fluoro-N-4'-(3-hy complex I or II in a synergistically effective amount. droxy-3-methylbut-1-yn-1-yl)biphenyl-2-yl-1,3-dimethyl 14. The method according to claim 13 further comprising 1H-pyrazole-4-carboxamide, 2-chloro-N-4'-(3-hydroxy-3- at least one additional fungicide (II), with the proviso that the methylbut-1-yn-1-yl)biphenyl-2-ylpyridine-3- biological control agent, fungicide (I) and fungicide (II) are carboxamide, 3-(difluoromethyl)-N-4'-(3-methoxy-3- not identical. methylbut-1-yn-1-yl)biphenyl-2-yl)-1-methyl-1H-pyrazole 15. Kit of parts comprising at least one biological control 4-carboxamide, 5-fluoro-N-4'-(3-methoxy-3-methylbut-1- agent selected from the group consisting of Bacillus chitino yn-1-yl)biphenyl-2-yl-1,3-dimethyl-1H-pyrazole-4- sporus AQ746 (NRRL Accession No. B-21618), Bacillus carboxamide, 2-chloro-N-4'-(3-methoxy-3-methylbut-1- mycoides AQ726 (NRRL Accession No. B-21664), Bacillus yn-1-yl)biphenyl-2-ylpyridine-3-carboxamide, (5-bromo pumilus (NRRL Accession No. B-30087), Bacillus pumilus AQ717 (NRRL Accession No. B-21662), Bacillus sp. AQ175 2-methoxy-4-methylpyridin-3-yl)(2,3,4-trimethoxy-6- (ATCC Accession No. 55608), Bacillus sp. AQ177 (ATCC methylphenyl)methanone, N-(2-(4-3-(4-chlorophenyl) Accession No. 55609), Bacillus sp. AQ178 (ATCC Accession prop-2-yn-1-yl)oxy-3-methoxyphenyl)ethyl-N2 No. 53522), Bacillus subtilis AQ743 (NRRL Accession No. (methylsulfonyl) valinamide, 4-oxo-4-(2-phenylethyl) B-21665), Bacillus subtilis AQ713 (NRRL Accession No. aminobutanoic acid, but-3-yn-1-yl {6-({(Z)-(1-methyl B-21661), Bacillus subtilis AQ153 (ATCC Accession No. 1H-tetrazol-5-yl)(phenyl)methylenelaminooxy)methyl 55614), Bacillus thuringiensis BD#32 (NRRL Accession No. pyridin-2-yl)carbamate, 4-Amino-5-fluorpyrimidin-2-ol B-21530), Bacillus thuringiensis AQ52 (NRRL Accession (mesomeric form: 6-Amino-5-fluorpyrimidin-2(1H)-on), No. B-21619), Muscodor albus 620 (NRRL Accession No. propyl 3,4,5-trihydroxybenzoate and oryzastrobin. 30547), Muscodor roseus A3-5 (NRRL Accession No. 8. The composition according to claim 1 additionally com 30548), Rhodococcus globerulus AQ719 (NRRL Accession prising at least one auxiliary selected from the group consist No. B-21663), Streptomyces galbus (NRRL Accession No. ing of extenders, Solvents, spontaneity promoters, carriers, 30232), Streptomyces sp. (NRRL Accession No. B-301.45), emulsifiers, dispersants, frost protectants, thickeners and Bacillus thuringiensis subspec. Kurstaki BMP 123, Bacillus adjuvants. subtilis AQ30002 (NRRL Accession No. B-50421), and Bacillus subtilis AQ30004 (NRRL Accession No. B-50455), 9. A seed treated with the composition according to claim and/or a mutant of these strains having all identifying char 1. acteristics of the respective strain, and/or a metabolite pro 10. A composition according to claim 1 capable of being duced by the respective strain that exhibits activity against used as fungicide and/or insecticide. insects, mites, nematodes and/or phytopathogens and at least 11. The composition according to claim 10 capable of one fungicide (I) selected from the group consisting of inhibi being used for reducing overall damage of plants and plant tors of the respiratory chain at complex I or II in a synergis parts as well as losses in harvested fruits or vegetables caused tically effective amount, in a spatially separated arrangement. by insects, mites, nematodes and/or phytopathogens. k k k k k