Addendum new products not listed in your 2007 catalog 2007

NorthernLights™ contents A new line of fl uorochromes for Flow Cytometry and IHC page 2 Secondary Antibodies for Immunohistochemistry Recombinant & Natural Proteins

Description Conjugate Catalog # Size page 3-5 Donkey Anti-Goat IgG, Cross-adsorbed NorthernLights-557 NL001 500 µg Polyclonal Antibodies Donkey Anti-Goat IgG, Cross-adsorbed NorthernLights-637 NL002 500 µg page 5-7 Donkey Anti-Goat IgG, Cross-adsorbed NorthernLights-493 NL003 500 µg Monoclonal Antibodies Donkey Anti-Rabbit IgG, Cross-adsorbed NorthernLights-557 NL004 500 µg Donkey Anti-Rabbit IgG, Cross-adsorbed NorthernLights-637 NL005 500 µg page 7-9 Donkey Anti-Rabbit IgG, Cross-adsorbed NorthernLights-493 NL006 500 µg Labeled Antibodies Donkey Anti-Mouse IgG, Cross-adsorbed NorthernLights-557 NL007 500 µg page 9 Donkey Anti-Mouse IgG, Cross-adsorbed NorthernLights-637 NL008 500 µg ELISA & Activity Assay Development Kits Donkey Anti-Mouse IgG, Cross-adsorbed NorthernLights-493 NL009 500 µg page 10 Streptavidin NL-493 NorthernLights-493 NL997 500 µg Primer Pairs Streptavidin NL-637 NorthernLights-637 NL998 500 µg Streptavidin NL-557 NorthernLights-557 NL999 500 µg page 10 Stem Cell & Cell Culture/Selection

Excitation/Emission Properties of NorthernLights Fluorochromes page 11 NorthernLights-493 493/514 ELISAs/Assays NorthernLights-557 557/574 NorthernLights-637 637/658 page 11 Cell-Based Assays Rat cortical stem cell differentiation was monitored using dual `NEW ! Phospho-ERK1/ERK2 (T202/Y204) immunocytochemistry. Neural progenitors were labeled with R&D Systems goat anti-rat nestin polyclonal antibody (Catalog # AF2736) and stained with R&D Systems donkey anti-goat NorthernLights-493 secondary antibody (Catalog # NL003; green). Differentiated neurons were labeled with R&D Systems neuron- specifi c mouse anti-β-III tubulin monoclonal antibody (TuJ-1; Catalog # MAB1195) and stained using R&D Systems donkey anti-mouse NorthernLights-557 secondary antibody (Catalog # NL007; red). Nuclei were counterstained with DAPI (blue). ® www.RnDSystems.com/go/NorthernLights

2007 CATALOG & ADDENDUM Recombinant & Natural Proteins

Protein Species Source Catalog # Size ASAH2/N-acylsphingosine Amidohydrolase-2 Mouse NS0 3558-AH-010 10 µg Biglycan Human NS0 2667-CM-050 50 µg CD31/PECAM-1 Mouse NS0 3628-PC-050 50 µg CD44/Fc Chimera Human NS0 3660-CD-050 50 µg CD48/SLAMF2 Human NS0 3644-CD-050 50 µg CRTAM/Fc Chimera (Class I-restricted T cell-associated Molecule) Human NS0 1695-CR-050 50 µg CRTAM/Fc Chimera (Class I-restricted T cell-associated Molecule) Mouse NS0 3687-CR-050 50 µg Dopa Decarboxylase/DDC Human Sf 21 3564-DC-010 10 µg EGF R/Fc Chimera Human NS0 344-ER-050 50 µg Fibronectin Fragment 4 Human NS0 3624-FN-050 50 µg GPVI Human NS0 3627-GP-050 50 µg KIR3DL2/Fc Chimera Human NS0 2878-KR-050 50 µg Latexin Mouse E. coli 3620-PI-010 10 µg LRIG1 (Leucine-rich Repeat and Ig-like Domain Protein 1) Mouse NS0 3688-LR-050 50 µg Methionine Aminopeptidase 1 Human Sf 21 3537-ZN-010 10 µg

Nogo-A/Fc Chimera aa 1026 - 1090 Rat Sf 21 3728-NG-050 50 µg Notch-1/Fc Chimera Human NS0 3647-TK-050 50 µg NOV/CCN3 Human NS0 1640-NV-050 50 µg NOV/CCN3 Mouse NS0 1976-NV-050 50 µg Slit3 Mouse NS0 3629-SL-050 50 µg Thrombospondin-4 Human CHO 2390-TH-050 50 µg TIM-1/KIM-1/HAVCR Human NS0 1750-TM-050 50 µg TIM-4 Human NS0 2929-TM-050 50 µg Trypsin 3/PRSS3 Human NS0 3710-SE-010 10 µg Trypsin 3/PRSS3 Mouse NS0 3565-SE-010 10 µg Active Trypsin 3/PRSS3 Human NS0 3714-SE-010 10 µg

2 For research use only. Not for use in diagnostic procedures. Polyclonal Antibodies

Antibody Species Type Catalog # Size ACE-2 Mouse Goat IgG AF3437 100 µg AMICA/JAML Human Goat IgG AF3449 100 µg Aminoacylase/ACY1 Mouse Goat IgG AF2700 100 µg Annexin A10 Human Goat IgG AF3544 100 µg Annexin A1/Annexin I Human/Mouse/Rat Goat IgG AF3770 100 µg APLP-2 (Amyloid Precursor-like Protein 2) Mouse Goat IgG AF3685 100 µg Apolipoprotein AI Human Goat IgG AF3664 100 µg B4GALT1 Human Goat IgG AF3609 100 µg BACE-2 Mouse Goat IgG AF2977 100 µg BMP-9 Human Goat IgG AF3209 100 µg Carboxypeptidase B1 Human Goat IgG AF2897 100 µg Cathepsin F Human Goat IgG AF2075 100 µg CD97 Mouse Goat IgG AF3734 100 µg Claspin Human Goat IgG AF3310 100 µg Coagulation Factor XIV/Protein C Human Goat IgG AF3349 100 µg Complement Component C1rLP Human Goat IgG AF3676 100 µg Contactin-5 Human Sheep IgG AF3030 100 µg CRELD2 (Cysteine-rich with EGF-like Domains 2) Human Goat IgG AF3555 100 µg CRELD2 (Cysteine-rich with EGF-like Domains 2) Mouse Goat IgG AF3686 100 µg Cubilin Human Sheep IgG AF3700 100 µg DPPA4 (Developmental Pluripotency-associated Protein 4) Human Sheep IgG AF3674 100 µg Endoglycan Mouse Goat IgG AF3534 100 µg EphA1 Mouse Goat IgG AF3034 100 µg Frk (Fyn-related Kinase) Human/Mouse/Rat Goat IgG AF3766 100 µg

C-Terminus GABAA R α1 Subunit Human/Rat/Bovine Rabbit IgG PPS022 100 µL N-Terminus GABAA R α1 Subunit Mouse/Rat Rabbit IgG PPS023 100 µL

GABAA R α2 Subunit Human/Mouse/Rat/Bovine Rabbit Serum PPS024 50 µL C-Terminus GABAA R α4 Subunit Human/Rat/Bovine Rabbit IgG PPS025 100 µL N-Terminus GABAA R α4 Subunit Rat Rabbit IgG PPS026 100 µL C-Terminus GABAA R α5 Subunit Mouse/Rat Rabbit IgG PPS027 100 µL C-Terminus GABAA R α6 Subunit Human/Rat/Bovine Rabbit IgG PPS028 100 µL C-Terminus GABAA R β1 Subunit Human/Rat/Bovine Rabbit IgG PPS030 100 µL C-Terminus GABAA R β2 Subunit Human/Rat/Bovine Rabbit IgG PPS031 100 µL C-Terminus GABAA R β3 Subunit Mouse/Rat Rabbit IgG PPS032 100 µL C-Terminus GABAA R γ2 Subunit Human/Mouse/Rat/Bovine Rabbit IgG PPS029 100 µL GFI-1 (Growth Factor Independent 1) Human Goat IgG AF3540 100 µg GLI-2 Mouse Goat IgG AF3635 100 µg continued on page 4

www.RnDSystems.com 3 Polyclonal Antibodies

Antibody Species Type Catalog # Size continued from page 3 GPVI (Platelet Glycoprotein VI) Human Sheep IgG AF3627 100 µg Granzyme B Human Goat IgG AF2906 100 µg HO-1/HMOX1/HSP32 Human/Mouse Goat IgG AF3776 100 µg HSP90 Human/Mouse/Rat Goat IgG AF3775 100 µg Iduronate 2-Sulfatase/IDS Mouse Goat IgG AF2486 100 µg IGSF4B Human Goat IgG AF3678 100 µg

IKKα Human/Mouse/Rat Sheep IgG AF3768 100 µg IL-5 Equine Goat IgG AF2470 100 µg IL-18/IL-1F4 Canine Goat IgG AF2924 100 µg KLF4 (Kruppel-like Factor 4) Human Goat IgG AF3640 100 µg KLF5 (Kruppel-like Factor 5) Human Goat IgG AF3758 100 µg LEDGF (Lens Epithelium-derived Growth Factor) Human Goat IgG AF3468 100 µg LIN-28 Human Goat IgG AF3757 100 µg LINGO-2 Human Goat IgG AF3679 100 µg Lipin 2 Human/Mouse/Rat Goat IgG AF3756 100 µg Lyp/PTPN22 Human Goat IgG AF3428 100 µg MGMT (O-6-methylguanine DNA Methyltransferase) Mouse Goat IgG AF3299 100 µg MMP-8 Rat Goat IgG AF3245 100 µg Myeloperoxidase Mouse Goat IgG AF3667 100 µg

Notch-2 intracellular domain Human Goat IgG AF3735 100 µg NTH1 Human Goat IgG AF2675 100 µg Park7/DJ-1 Mouse Goat IgG AF3668 100 µg Peroxiredoxin 5 Human/Mouse Goat IgG AF3774 100 µg Plexin B1 Human Goat IgG AF3749 100 µg Podoplanin Human Sheep IgG AF3670 100 µg PSP94 (Prostate Secretory Protein) Human Goat IgG AF3780 100 µg RAGE Rat Goat IgG AF1616 100 µg RalA Human/Mouse/Rat Goat IgG AF3270 100 µg Rap1A/B Human/Mouse/Rat Goat IgG AF3767 100 µg RGM-B (Repulsive Guidance Molecule B) Human Goat IgG AF3630 100 µg RGM-C (Repulsive Guidance Molecule C) Mouse Goat IgG AF3634 100 µg RGM-C (Repulsive Guidance Molecule C) Human Goat IgG AF3720 100 µg R-Spondin 2 Human Goat IgG AF3266 100 µg R-Spondin 3 Human Goat IgG AF3500 100 µg Serpin E2/PN1 Human Goat IgG AF2980 100 µg Phospho-SHP-2 (Y542) Human/Mouse Rabbit IgG AF3790 100 µg Siglec-6 Human Sheep IgG AF2859 100 µg Sleeping Beauty Transposase Multi-species Goat IgG AF2798 100 µg SOX21 Human Goat IgG AF3538 100 µg Phospho-STAT6 (Y641) Human Rabbit IgG AF3717 50 µg Sulfatase Modifying Factor 1/SUMF1 Human Goat IgG AF3680 100 µg

4 For research use only. Not for use in diagnostic procedures. Polyclonal Antibodies

Antibody Species Type Catalog # Size Phospho-Synapsin I (S603) Rat Rabbit IgG PPS036 100 µL Syndecan-3 Human Goat IgG AF3539 100 µg TCAM-1 (Testicular Cell Adhesion Molecule) Mouse Goat IgG AF3546 100 µg TFPI-2 Human Goat IgG AF2545 100 µg

Latent TGF-β Binding Protein 4 Mouse Goat IgG AF2885 100 µg Thioredoxin-like 5/TRP14 Mouse/Rat Goat IgG AF3769 100 µg TIM-1/KIM-1/HAVCR Rat Goat IgG AF3689 100 µg TRIM5 (Tripartite Motif-containing 5) Human Goat IgG AF3684 100 µg TSC22 Mouse/Rat Goat IgG AF3779 100 µg Wnt-9b Mouse Goat IgG AF3669 100 µg Xg/PBDX Human Goat IgG AF3699 100 µg XPA (Xeroderma Pigmetosum A) Human Goat IgG AF3416 100 µg YY1 (Ying Yang 1) Human Goat IgG AF3784 100 µg ZNF24 (Zincfi nger protein 24) Human Goat IgG AF3637 100 µg

Monoclonal Antibodies

Antibody Species Clone Number Catalog # Size AGTR-2 (Angiotensin II Type 2 Receptor) Human 364805 MAB3659 100 µg

AMPKα1 Human/Mouse/Rat 362532 MAB3197 100 µg Angiopoietin-like 4 Human 317223 MAB3485 100 µg B7-H1/PD-L1 Mouse 179711 MAB1019 100 µg

βIG-H3 Human 348506 MAB2935 100 µg CAR/NR1I3 (Constitutive Androstane Receptor) Human/Mouse 331930 MAB3724 100 µg Carbonic Anhydrase IV Human 310415 MAB21861 100 µg CD38 Human 240726 MAB24041 100 µg CD48/SLAMF2 Mouse 331504 MAB3327 100 µg CD200 R1 Mouse 330502 MAB2554 100 µg Chordin-Like 2 Mouse 328905 MAB2520 100 µg Claudin-6 Human 342927 MAB3656 100 µg

Clusterin α Chain Mouse 327020 MAB2747 100 µg COX-1 Human 345305 MAB3740 100 µg CRISP-3 (Cysteine-rich Secreted Protein) Human 295208 MAB23972 100 µg DPPA5/ESG1 (Developmental Pluripotency-associated Protein 5) Human 319606 MAB3125 100 µg DSPG3 (Dermatan Sulfate Proteoglycan 3) Human 329707 MAB3110 100 µg EDG-5 Human 368510 MAB3649 100 µg Phospho-EGF R (Y1068) Human 338324 MAB3570 100 µg continued on page 6

www.RnDSystems.com 5 Monoclonal Antibodies

Antibody Species Clone Number Catalog # Size continued from page 5 EphB1 Rat 88512 MAB5421 100 µg Exostosin-like 2/EXTL2 Mouse 339509 MAB2536 100 µg FATP4 (Fatty Acid Transport Protein 4) Human 342142 MAB3650 100 µg

Fgr tyrosine kinase Human/Mouse/Rat 349316 MAB3207 100 µg

Fgr tyrosine kinase Human 349331 MAB32071 100 µg FLRG (Follistatin-related Gene) Human 206714 MAB1288 100 µg FPR1 (Formyl Peptide Receptor 1) Human 350418 MAB3744 100 µg Fyn Human/Mouse/Rat 361228 MAB3574 100 µg GASP-1/WFIKKNRP Human 288518 MAB20701 100 µg GDF-3 Propeptide Mouse 356613 MAB3719 100 µg Glypican 3 Human 307801 MAB2119 100 µg GPR15 (G Protein-coupled Receptor 15) Human 367902 MAB3654 100 µg Histone H2AX Human/Mouse/Rat 322105 MAB3406 50 µg

IFN-γ Bovine 345025 MAB2300 100 µg IGSF2/CD101 Mouse 307707 MAB3368 100 µg

IL-1F6/FIL1 ε Mouse 275340 MAB22971 100 µg IL-1F10/IL-1HY2 Human 316709 MAB2427 100 µg IL-1 R4/ST2L Mouse 245707 MAB10041 500 µg IL-6 Feline 341031 MAB23051 100 µg IL-31 Human 308202 MAB28241 100 µg

Integrin β2/CD18 Mouse 313903 MAB2618 100 µg Kallikrein 7 Human 333931 MAB2624 500 µg

Klotho β Human/Mouse 337208 MAB3738 100 µg LAG-3 Human 333210 MAB2319 100 µg LAIR1 (Leukocyte-associated Ig-like Human 342219 MAB2664 100 µg Receptor 1) Langerin/CD207 Human 343828 MAB2088 100 µg MafB Human/Rat 372239 MAB3810 100 µg Melanocortin 3R/MC3R Mouse 345404 MAB3737 100 µg Mast Cell Protease-6 Mouse 286820 MAB3736 100 µg MEPE (Matrix Extracellular Phosphoglycoprotein) Human 341611 MAB3140 100 µg MGMT (O-6-methylguanine DNA Methyltransferase) Mouse 300008 MAB3299 100 µg MIA (Melanoma-inhibitory Activity) Human 294203 MAB20501 100 µg MMP-11 Human 135421 MAB3657 100 µg NCAM-1/CD56 Human 301021 MAB24081 100 µg OBCAM Human 341723 MAB27771 100 µg p53 Human/Mouse/Rat 184727 MAB13551 100 µg Phospho-p53 (S15) Human 261336 MAB18392 100 µg CXCL4/PF4 Human 170138 MAB7952 100 µg

PLCγ-1 Human/Mouse/Rat 346130 MAB3288 100 µg Relaxin-2 Human 349102 MAB2804 100 µg SHBG (Sex Hormone-binding Globulin) Human 336711 MAB2656 100 µg

6 For research use only. Not for use in diagnostic procedures. Monoclonal Antibodies

Antibody Species Clone Number Catalog # Size SR-AI/MSR1 Human 351615 MAB2708 100 µg Syndecan-1/CD138 Mouse 320611 MAB29661 100 µg TIM-1/KIM-1/HAVCR Human 219217 MAB17501 100 µg TIM-3 Human 344823 MAB2365 100 µg TLR9 Human 229106 MAB3658 100 µg WASP Human 336918 MAB30701 100 µg Yes Human/Mouse/Rat 339827 MAB32051 100 µg

Labeled Antibodies Biotinylated Antibodies

Antibody Species Type Catalog # Size Aminoacylase/ACY1 Human Goat IgG BAF2900 50 µg Aminopeptidase P2/XPNPEP2 Mouse Goat IgG BAF2494 50 µg Angiopoietin-like 4 Human Goat IgG BAF3485 50 µg Apolipoprotein B Human Goat IgG BAF3556 50 µg C1qTNF4 Human Goat IgG BAF3456 50 µg Carbonic Anhydrase XII Human Goat IgG BAF2190 50 µg Choline Acetyltransferase/ChAT Human Goat IgG BAF3447 50 µg CL-P1/COLEC12 Mouse Goat IgG BAF3130 50 µg Coagulation Factor III/Tissue Factor Human Goat IgG BAF2339 50 µg Collagen II Mouse Sheep IgG BAF3615 50 µg EphA2 Human Goat IgG BAF3035 50 µg Galectin-9 Mouse Goat IgG BAF3535 50 µg GLI-2 Human Sheep IgG BAF3526 50 µg IL-6 Equine Goat IgG BAF1886 50 µg Kallikrein 5 Human Goat IgG BAF1108 50 µg MCAM/CD146 (Melanoma Cell Adhesion Molecule) Rat Goat IgG BAF3250 50 µg Mesothelin Propeptide Human Goat IgG BAF3448 50 µg Meteorin Mouse Goat IgG BAF3475 50 µg MFRP (Membrane-type Frizzled-related Protein) Mouse Goat IgG BAF3445 50 µg Nidogen-2 Human Goat IgG BAF3385 50 µg OCIL/CLEC2d (Osteoclast Inhibitory Lectin 1) Mouse Goat IgG BAF3376 50 µg OCILRP2/CLEC2i Mouse Goat IgG BAF3370 50 µg Pygopus-2 Human Goat IgG BAF3616 50 µg SOX10 Human Goat IgG BAF2864 50 µg VE-Statin Mouse Goat IgG BAF3089 50 µg Tenascin C Human Goat IgG BAF3358 50 µg TFPI (Tissue Factor Pathway Inhibitor) Human Goat IgG BAF2974 50 µg Wnt-8a Mouse Goat IgG BAF2248 50 µg

www.RnDSystems.com 7 Labeled Antibodies Fluorochrome-labeled Antibodies

Antibody Species Label Catalog # Size ADAM10 Human APC IC1427A 100 Tests ADAM10 Human Fluorescein IC1427F 100 Tests ADAM10 Human PE IC1427P 100 Tests ADAM15 Human Fluorescein IC935F 100 Tests ADAM15 Human PE IC935P 100 Tests BAFF R/TNFRSF13C Mouse Fluorescein FAB1755F 100 Tests BMPR-IA/ALK-3 Human Fluorescein FAB346F 100 Tests CCR10 Mouse PE FAB2815P 100 Tests CD31/PECAM-1 Human APC FAB3567A 100 Tests CD31/PECAM-1 Human Biotin FAB3567B 100 Tests CD31/PECAM-1 Human Fluorescein FAB3567F 100 Tests CD31/PECAM-1 Human PE FAB3567P 100 Tests CD44v6 Human APC FAB3660A 100 Tests CD68/SR-D1 Human APC IC20401A 100 Tests CD68/SR-D1 Human Fluorescein IC20401F 100 Tests DEP-1/CD148 (Density-enhanced Protein Tyrosine Phosphatase Human APC FAB1934A 100 Tests 1)

Fcγ RIII/CD16 Mouse Fluorescein FAB19601F 100 Tests Fcγ RIII/CD16 Mouse PE FAB19601P 100 Tests FPRL1 (Formyl Peptide Receptor-like 1) Human APC FAB3479A 100 Tests FPRL1 (Formyl Peptide Receptor-like 1) Human Fluorescein FAB3479F 100 Tests FPRL1 (Formyl Peptide Receptor-like 1) Human PE FAB3479P 100 Tests

CXCL1/GROα Human APC IC275A 100 Tests IL-21 R Mouse PE FAB5961P 100 Tests CCL13/MCP-4 Human Fluorescein IC327F 100 Tests NG2/MCSP Human APC FAB2585A 100 Tests NG2/MCSP Human Fluorescein FAB2585F 100 Tests NG2/MCSP Human PE FAB2585P 100 Tests NKG2D Mouse APC FAB1547A 100 Tests NKG2D Mouse Fluorescein FAB1547F 100 Tests NKG2D Mouse PE FAB1547P 100 Tests OSM (Oncostatin M) Human APC IC2951A 100 Tests OSM (Oncostatin M) Human Fluorescein IC2951F 100 Tests OSM (Oncostatin M) Human PE IC2951P 100 Tests PD-1 Human Fluorescein FAB1086F 100 Tests PD-1 Human PE FAB1086P 100 Tests TLR2 Mouse APC FAB1530A 100 Tests TLR4 Human Fluorescein FAB14781F 100 Tests TLR4 Human PE FAB14781P 100 Tests TLR4 Mouse APC IC2759A 100 Tests TrkA Human APC FAB1751A 100 Tests TrkA Human PE FAB1751P 100 Tests

8 For research use only. Not for use in diagnostic procedures. Labeled Antibodies Fluorochrome-labeled Antibodies

Antibody Species Label Catalog # Size VEGF R1/Flt-1 Mouse APC FAB4711A 100 Tests VEGF R1/Flt-1 Mouse PE FAB4711P 100 Tests

ELISA & Activity Assay Development Kits DuoSet® ELISA Development Systems

Product Species Catalog # Reagents for* Amphiregulin Mouse DY989 15 Plates BCMA/TNFRSF17 Human DY193 15 Plates E-Cadherin Mouse DY748 15 Plates Cardiotrophin-1 (CT-1) Mouse DY438 15 Plates CD27/TNFRSF7 Mouse DY574 15 Plates Chordin Mouse DY758 15 Plates Complement Factor D Human DY1824 15 Plates Galectin-1 Mouse DY1245 15 Plates Galectin-7 Human DY1339 15 Plates CCL14a/HCC-1 Human DY1578 15 Plates IL-6 sR Mouse DY1830 15 Plates

IL-29/IFN-λ1 Human DY1598 15 Plates Neprilysin/CD10 Human DY1182 15 Plates Neprilysin/CD10 Mouse DY1126 15 Plates Pentraxin 3/TSG-14 Mouse DY2166 15 Plates Prolactin Mouse DY1445 15 Plates TACE/ADAM17 Human DY930 15 Plates TWEAK R/TNFRSF12 Mouse DY1610 15 Plates *Also available in 45 plate Economy Packs.

DuoSet IC Intracellular ELISAs & Activity Assays Product Species Catalog # Reagents for* Phospho-Flt-3/Flk-2 Human DYC368-2 1 Plate Total Flt-3/Flk-2 Human DYC912-2 1 Plate Phospho-MSP R/Ron Human DYC1947-2 1 Plate Total Tie-2 Mouse DYC762-2 1 Plate Phospho-VEGF R3/Flt-4 Human DYC2724-2 1 Plate Total VEGF R3/Flt-4 Human DYC3491-2 1 Plate *Also available in 5 plate packs and 15 plate Economy Packs.

www.RnDSystems.com 9 Primer Pairs Primer Pair Species Catalog # Size

α-Fetoprotein Mouse/Rat RDP-337-025 25 Tests Brachyury Mouse/Rat RDP-343-025 25 Tests DPPA5/ESG1 (Developmental Pluripotency-associated Protein 5) Mouse/Rat RDP-333-025 25 Tests GATA-4 Mouse/Rat RDP-345-025 25 Tests

HNF-3β/FoxA2 (Hepatic Nuclear Factor 3β) Mouse/Rat RDP-340-025 25 Tests Nanog Mouse/Rat RDP-334-025 25 Tests Oct-3/4 Mouse/Rat RDP-335-025 25 Tests Otx2 Mouse/Rat RDP-342-025 25 Tests PDX-1/IPF1 (Pancreatic Duodenal Homeobox Factor 1) Mouse/Rat RDP-338-025 25 Tests SOX2 Mouse/Rat RDP-336-025 25 Tests SOX17 Mouse/Rat RDP-339-025 25 Tests Stella/Dppa3 Mouse/Rat RDP-344-025 25 Tests TP63/TP73L Mouse/Rat RDP-341-025 25 Tests

Stem Cell & Cell Culture/Selection Stem Cell Kits

Product Species Catalog # Size Pluripotent Stem Cell Assessment Primer Pair Panel Mouse/Rat SC015 1 Kit

MagCellect™ Cell Isolation Kits: Magnetic Nanoparticles Product Species Catalog # Size VEGF R2/KDR/Flk-1 Human MAGH108 1 Kit

10 For research use only. Not for use in diagnostic procedures. ELISAs/Assays Quantikine® Colorimetric Sandwich ELISAs

Analyte Species Sensitivity Range Catalog # Size IL-22 Mouse/Rat 4.6 pg/mL 15.6-1000 pg/mL M2200 1 Kit IL-23 Human 5.9 pg/mL 15.6-1000 pg/mL D2300 1 Kit Surveyor™ IC (Intracellular) ELISAs

Product Species Catalog # Size Phospho-ERK1/ERK2 Human/Mouse/Rat SUV1018 1 Kit

Cell-Based Assays

Product Species Catalog # Size Phospho-EGF R (Y1068) Human KCB1095 1 Kit Phospho-ERK1/ERK2 (T202/Y204) Human/Mouse/Rat KCB1018 1 Kit

Phospho-PDGF Rβ (Y751) Human KCB1767 1 Kit Phospho-PDGF Rβ (Y1021) Human KCB2316 1 Kit

New Cell-Based Assay: Phospho-ERK1/ERK2 (T202/Y204)

R&D Systems’ new phospho-ERK1/ERK2 (T202/Y204) Cell-Based 1500

ELISA (Catalog # KCB1018) is the fi rst fl uorescent Cell-Based 025102030Time (minutes) ELISA to allow researchers to screen for both phospho- and total Phospho-ERK1/ERK2 ERK1/ERK2 in the same well. For the most accurate accounting of well-to-well discrepancies in cell number, the phospho-ERK ERK1/ERK2 fl uorescence in each well is normalized to that of total ERK fl uorescence from the same well. This kit contains the 1000 components required for running an ELISA using two different fl uorogenic substrates to measure phosphorylated and total ERK1/ERK2 in the context of a whole cell. This simple and effi cient assay eliminates the need to prepare cell lysates and therefore is more amenable to high-throughput kinase signaling and inhibition investigation. Phosphorylation of ERK1/ERK2 (Normalized RFUs) 500 0 2 5 10 20 30 >> Obtain results from as few as 10,000 cells/well Time (minutes) >> Minimal sample handling - all steps are performed in the same well Figure 1. Time course of EGF-induced ERK1/ERK2 phosphorylation. A431 human epider- moid carcinoma cells were cultured in 96-well plates and treated with 100 ng/mL of recom- >> Read total and phosho-ERK levels simultaneously binant human EGF (R&D Systems, Catalog # 236-EG) for the indicated times. After fi xation of cells in the wells, phosphorylation of ERK1/ERK2 on T202/Y204 was determined using this Phospho-ERK1/ERK2 (T202/Y204) Cell-Based ELISA. Values represent mean ± range of dupli- cate determinations. Analysis of ERK1/ERK2 phosphorylation on T202/Y204 by Western blot- ting is also shown (inset).

www.RnDSystems.com 11 2007 CATALOG & ADDENDUM

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