DOCSLIB.ORG

Abstract Book.Cdr

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Abstract Book.Cdr Peter Hepler University of Massachusets. PETER K. HEPLER is the Ray Ethan Torrey Professor, Emeritus in the Biology Department at the University of Massachuses, Amherst, MA. He has been invesgang the cytoskeleton in plants for over 50 years. He also studies the role of calcium and protons during signal transducon, and is parcularly interested in the interplay between these ions and the cytoskeleton. Earlier work focused on the mechanism and control of cell division, with parcular emphasis on cytokinesis. More recently aenon has been given to studies on the mechanism and regulaon of pollen tube growth. In his work Dr. Hepler uses a variety of microscopical methods and approaches including both light and electron microscopy. In more recent years an emphasis has been given to the examinaon of structures and dynamic processes as observed in living cells. He and co-workers have been involved in developing the methods and use of fluorescent analog cytochemistry, and as well as transient expression of fluorescent reporters to examine the cytoskeleton in living plant cells. He has also championed the use of raometric ion imaging for examining the locaon and dynamics of calcium and protons during plant growth and development. Talk: Polarized growth of the pollen tube: a role for ions and acn. Peter K. Hepler Biology Department, University of Massachuses, Amherst, MA 01003. Pollen tubes are polarized, p-growing cells that deliver sperm cells to the egg apparatus in higher plants. Their growth is essenal for sexual reproducon, and for the development of the fruits, nuts and seeds that we eat. Herein I report on the mechanism of lily pollen tube growth. Rapid, polarized growth depends on the synthesis and localized secreon of cell wall components, which are packaged in vesicles, and transported apically along acn microfilaments. Controlling factors include acn, which is organized as a corcal fringe in the pollen tube p, plus apical gradients of calcium and pH. All these factors demonstrate an oscillatory behavior that correlates with the oscillaon in growth rate. Quantave studies indicate that increases in secreon, the alkaline band, and acn polymerizaon ancipate the increases in growth rate, whereas increases in the calcium gradient follow the increases in growth rate. To gain further insight we reversibly inhibited growth with KCN, and followed the effects on calcium, pH, secreon and the apical acn fringe. During recovery the calcium gradient reappears ≈2.5 minutes before growth restarts. However the alkaline band precedes the increase in calcium. Secreon follows a paern similar to the alkaline band, re-emerging before KCN has been removed. KCN also degrades the acn fringe. Secreon, which restarts in the absence of the fringe, inially lacks focus and causes ballooning of the apex. However, with the re-appearance of the fringe, growth becomes polarized. I suggest that the acn fringe delivers vesicles to their secretory locus, thereby giving polarity to the growing pollen tube, while the proton ATPase establishes the membrane potenal and ion gradients that control the transport of nutrients necessary for growth. Luis Cárdenas Instuto de Biotecnología, UNAM. LUIS CÁRDENAS is a tenured professor at the Instute of Biotechnology (IBT) of the Naonal Autonomous University of México (UNAM) and a researcher at the Plant Molecular Biology Department. He earned his PhD from IBT-UNAM and then visited Peter K. Hepler´s lab in the USA for a postdoctoral training. He described that Nod factors (NFs) from Rhizobium induced an extensive fragmentaon of the acn cytoskeleton in living root hair cells. This study, which was as a cover story in Plant Physiology, has been cited over 100 mes and is emerging as a benchmark for further work on the structure of acn in response to NFs. These studies formed the basis for a review in Plant Physiology in 2000, which was highlighted as an Editors' Choice in Science. Perhaps his most insighul work was the discovery that reduced NAD, an important energy co-enzyme associated with mitochondria, oscillated during pollen tube growth, with oxidized NAD ancipang the increase in growth rate. These studies led to the hypothesis that oscillaons in metabolism iniate cell growth. This work was published in Plant Physiology and connues to be an emerging interesng aspect of plant cell growth. This contribuon was singled out by Faculty of 1000 as an important contribuon. He was awarded with the Alfonso Caso medal from UNAM as the best PhD student of his generaon, and his thesis awarded the Weisman prize from the Mexican Academy of Science. He was also awarded an American Society of Microbiology (ASM) fellowship to do summer training in Simon Gilroy's lab. At the moment he is interested in life cell imaging, polar growth, differenaon, and plant– microbe interacon. His work is fully supported by DGAPA UNAM and CONACyT, the two main sources for financial support. Talk: Reacve oxygen species in plant root hair cells as key regulators of the symbioc interacons and in pollen tubes as growth rate modulators. Cárdenas, L.1, Hernández-Barrera, A.1, Velarde-Buendía, A., Sánchez, R.1, Johnson E.2, Wu, H.M.2, Quinto, C.1, Cheung, A.2. 1Instuto de Biotecnología, Universidad Nacional Autónoma de México, Ap. Postal 510-3 Cuernavaca, Morelos, México. [email protected] 2Department of Biochemistry and Molecular Biology, University of Massachuses, Amherst. In plant cells ROS accumulaon have been involved in several processes such as: development, hypersensive response, hormonal percepon, gravitropism and stress response. In guard cells from Vicia faba it has been shown to regulates the opening of stomata and in root hair cells from Arabidopsis ROS levels generate and maintain an apical calcium gradient and it has been proposed to play a key role in the cell wall remodeling during polar growth in pollen tubes and other p growing cells. NADPH oxidases have emerged as the main source of ROS to sustain the polar growth since mutaons in this genes impairs the ROS generaon and root hair development. Furthermore, ROS have emerged as a key regulator during the mutualisc interacons and silencing or overexpression of ROS generang enzymes such as NADPH oxidases affect the onset of nodulaon and mycorrhizaon. Pollen tubes and root hairs exhibit an oscillatory growth with phase of high and low growth rate. It has been shown that extracellular ROS producon also oscillate with a similar frequency, but out of phase. However, a clear analysis of intracellular ROS dynamic has not been depicted. Herein we report a new molecular probe to depict the ROS dynamic during root hair cell and pollen tube apical growth. Hyper is a new generated GFP fused to the OxyR domain that result in a hydrogen peroxide specific probe. This molecular probe was expressed in root hair cells from Arabidopsis and tobacco pollen tubes . By using high resoluon microscopy we depicted an apical H₂O₂ gradient at the p dome where the polar growth occur, furthermore we were able to visualize dynamic ROS oscillaons in root hair cells, which are couple to growth. In pollen tubes we also found a parcular ROS distribuon, with clear oscillaons couple to growth fluctuaons. In both p growing cells, the apical regions are the site where the more dynamic ROS changes were observed, furthermore we describe the localizaon of the NADPH oxidase in membrane lipid ra, suggesng a pivotal role in polar growth. Reinhard Fischer Karlsruhe Instute of Technology. REINHARD FISCHER is a Full Professor at the Karlsruhe Instute of Technology (KIT) in Karlsruhe, Germany. He is leading an independent research group on fungal molecular biology since 1994 and is internaonally well-recognized. He and his research team analyzes different aspects of fungal biology. Main fields are the photoresponse of Aspergillus nidulans, where he discovered a role for phytochrome in fungi. Another focus is the cell- and molecular biological analysis of polarized growth. In an applied research field, he analyzes the secondary metabolism of Alternaria alternata to discover novel compounds and understand the genec regulaon of the involved genes. Recently, he started a new line of research with the analysis of the molecular biology of nematode-trapping fungi. Reinhard Fischer has served as editor or associate editor for several journals such as Fungal Genecs and Biology (2000-2012), Molecular Microbiology (since 2005), FEMS Microbiol. Leers (2005-2007), Molecular Genomics and Genecs (since 2006), Eukaryoc Cell (2013-2015), mSphere (since 2016), and mBio (since 2016). He was the organizer of the annual meeng of the German Microbiological Society (VAAM)(1500 parcipants) in 2011 and of the internaonal meeng on Fungal Biology (FBC)(300 parcipants) in 2013. He is elected member of the grant review panel for Microbiology, Immunology and Virology at the German Science Foundaon (DFG) since 2012, re-elected 2016. He is liaison professor of the Studiensung des deutschen Volkes since 2009. He has educated 35 Ph.D. students and more than 40 Diploma- or Master students. Talk: Septal microtubule-organizing centers of Aspergillus nidulans share proteins with the outer plaque of spindle pole bodies and are anchored at septa through the disordered protein Spa10. Ying Zhang1, Xiaolei Gao1, Raphael Manck1, Marjorie Schmid1, Aysha H. Osmani2, Stephen A. Osmani2, Norio Takeshita1, 3 and Reinhard Fischer11* 1Karlsruhe Instute of Technology (KIT) - South Campus, Instute for Applied Biosciences, Dept. of Microbiology Hertzstrasse 16 D-76187 Karlsruhe Phone: +49-721-6084-4630 Fax: +49-721-6084-4509 E-mail: reinhard.fi[email protected] homepage: www.iab.kit.de Microtubule-organizing centers (MTOCs), such as centrosomes or spindle-pole bodies (SPBs) of fungi, are large, mul-subunit protein complexes. In the model fungus Aspergillus nidulans septum-associated MTOCs (sMTOCs) nucleate microtubules in addion to SPBs. The structure of sMTOCs is poorly understood and how they are targeted to septa is unknown.
Load more

Recommended publications
  • Plant Molecular Farming: a Viable Platform for Recombinant Biopharmaceutical Production
    plants Review Plant Molecular Farming: A Viable Platform for Recombinant Biopharmaceutical Production Balamurugan Shanmugaraj 1,2, Christine Joy I. Bulaon 2 and Waranyoo Phoolcharoen 1,2,* 1 Research Unit for Plant-Produced Pharmaceuticals, Chulalongkorn University, Bangkok 10330, Thailand; [email protected] 2 Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences Chulalongkorn University, Bangkok 10330, Thailand; [email protected] * Correspondence: [email protected]; Tel.: +66-2-218-8359; Fax: +66-2-218-8357 Received: 1 May 2020; Accepted: 30 June 2020; Published: 4 July 2020 Abstract: The demand for recombinant proteins in terms of quality, quantity, and diversity is increasing steadily, which is attracting global attention for the development of new recombinant protein production technologies and the engineering of conventional established expression systems based on bacteria or mammalian cell cultures. Since the advancements of plant genetic engineering in the 1980s, plants have been used for the production of economically valuable, biologically active non-native proteins or biopharmaceuticals, the concept termed as plant molecular farming (PMF). PMF is considered as a cost-effective technology that has grown and advanced tremendously over the past two decades. The development and improvement of the transient expression system has significantly reduced the protein production timeline and greatly improved the protein yield in plants. The major factors that drive the plant-based platform towards potential competitors for the conventional expression system are cost-effectiveness, scalability, flexibility, versatility, and robustness of the system. Many biopharmaceuticals including recombinant vaccine antigens, monoclonal antibodies, and other commercially viable proteins are produced in plants, some of which are in the pre-clinical and clinical pipeline.
    [Show full text]
  • Therapeutic Recombinant Protein Production in Plants: Challenges and Opportunities
    Received: 25 April 2019 | Revised: 23 July 2019 | Accepted: 20 August 2019 DOI: 10.1002/ppp3.10073 REVIEW Therapeutic recombinant protein production in plants: Challenges and opportunities Matthew J. B. Burnett1 | Angela C. Burnett2 1Yale Jackson Institute for Global Affairs, New Haven, CT, USA Societal Impact Statement 2Brookhaven National Laboratory, Upton, Therapeutic protein production in plants is an area of great potential for increasing NY, USA and improving the production of proteins for the treatment or prevention of disease Correspondence in humans and other animals. There are a number of key benefits of this technique Angela C. Burnett, Brookhaven National for scientists and society, as well as regulatory challenges that need to be overcome Laboratory, Upton, NY, USA. Email: [email protected] by policymakers. Increased public understanding of the costs and benefits of thera‐ peutic protein production in plants will be instrumental in increasing the acceptance, Funding information Biotechnology and Biological Sciences and thus the medical and veterinary impact, of this approach. Research Council; Margaret Claire Ryan Summary Fellowship Fund at the Yale Jackson Institute for Global Affairs; U.S. Department Therapeutic recombinant proteins are a powerful tool for combating many diseases of Energy, Grant/Award Number: DE‐ which have previously been hard to treat. The most utilized expression systems are SC0012704 Chinese Hamster Ovary cells and Escherichia coli, but all available expression sys‐ tems have strengths and weaknesses regarding development time, cost, protein size, yield, growth conditions, posttranslational modifications and regulatory approval. The plant industry is well established and growing and harvesting crops is easy and affordable using current infrastructure.
    [Show full text]
  • Eukaryotic Expression
    Molecular Biology Problem Solver: A Laboratory Guide. Edited by Alan S. Gerstein Copyright © 2001 by Wiley-Liss, Inc. ISBNs: 0-471-37972-7 (Paper); 0-471-22390-5 (Electronic) 16 Eukaryotic Expression John J. Trill, Robert Kirkpatrick, Allan R. Shatzman, and Alice Marcy Section A: A Practical Guide to Eukaryotic Expression . 492 Planning the Eukaryotic Expression Project . 493 What Is the Intended Use of the Protein and What Quantity Is Required? . 493 What Do You Know about the Gene and the Gene Product? . 496 Can You Obtain the cDNA? . 497 Expression Vector Design and Subcloning . 498 Selecting an Appropriate Expression Host . 501 Selecting an Appropriate Expression Vector . 506 Implementing the Eukaryotic Expression Experiment . 511 Media Requirements, Gene Transfer, and Selection . 511 Scale-up and Harvest . 514 Gene Expression Analysis . 515 Troubleshooting . 517 Confirm Sequence and Vector Design . 517 Investigate Alternate Hosts . 519 A Case Study of an Expressed Protein from cDNA to Harvest.......................................... 519 Summary . 521 Section B: Working with Baculovirus . 521 Planning the Baculovirus Experiment . 521 491 Is an Insect Cell System Suitable for the Expression of Your Protein? . 521 Should You Express Your Protein in an Insect Cell Line or Recombinant Baculovirus? . 522 Procedures for Preparing Recombinant Baculovirus . 524 Criteria for Selecting a Transfer Vector . 524 Which Insect Cell Host Is Most Appropriate for Your Situation? . 525 Implementing the Baculovirus Experiment . 527 What’s the Best Approach to Scale-Up? . 527 What Special Considerations Are There for Expressing Secreted Proteins? . 527 What Special Considerations Are There for Expressing Glycosylated Proteins? . 528 What Are the Options for Expressing More Than One Protein?....................................
    [Show full text]
  • Recent Advances in Genome Editing Tools in Medical Mycology Research
    Journal of Fungi Review Recent Advances in Genome Editing Tools in Medical Mycology Research Sanaz Nargesi 1 , Saeed Kaboli 2,3,* , Jose Thekkiniath 4,*, Somayeh Heidari 5 , Fatemeh Keramati 6, Seyedmojtaba Seyedmousavi 5,7 and Mohammad Taghi Hedayati 1,5,* 1 Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari 481751665, Iran; [email protected] 2 Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan 4513956111, Iran 3 Cancer Gene Therapy Research Center, Zanjan University of Medical Sciences, Zanjan 4513956111, Iran 4 Fuller Laboratories, 1312 East Valencia Drive, Fullerton, CA 92831, USA 5 Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari 481751665, Iran; [email protected] (S.H.); [email protected] (S.S.) 6 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia 5756151818, Iran; [email protected] 7 Clinical Center, Microbiology Service, Department of Laboratory Medicine, National Institutes of Health, Bethesda, MD 20892, USA * Correspondence: [email protected] (S.K.); [email protected] (J.T.); [email protected] (M.T.H.) Abstract: Manipulating fungal genomes is an important tool to understand the function of tar- get genes, pathobiology of fungal infections, virulence potential, and pathogenicity of medically important fungi, and to develop novel diagnostics and therapeutic targets. Here, we provide an overview of recent advances in genetic manipulation techniques used in the field of medical mycol- Citation: Nargesi, S.; Kaboli, S.; ogy. Fungi use several strategies to cope with stress and adapt themselves against environmental Thekkiniath, J.; Heidari, S.; Keramati, effectors. For instance, mutations in the 14 alpha-demethylase gene may result in azole resistance in F.; Seyedmousavi, S.; Hedayati, M.T.
    [Show full text]
  • An Improved Syringe Agroinfiltration Protocol to Enhance Transformation Efficiency by Combinative Use of 5-Azacytidine, Ascorbat
    plants Article An Improved Syringe Agroinfiltration Protocol to Enhance Transformation Efficiency by Combinative Use of 5-Azacytidine, Ascorbate Acid and Tween-20 Huimin Zhao 1, Zilong Tan 2, Xuejing Wen 2 and Yucheng Wang 1,2,* 1 State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China; [email protected] 2 Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; [email protected] (Z.T.); [email protected] (X.W.) * Correspondence: [email protected]; Tel.: +86-451-82190607 Academic Editor: Milan S. Stankovic Received: 1 January 2017; Accepted: 9 February 2017; Published: 14 February 2017 Abstract: Syringe infiltration is an important transient transformation method that is widely used in many molecular studies. Owing to the wide use of syringe agroinfiltration, it is important and necessary to improve its transformation efficiency. Here, we studied the factors influencing the transformation efficiency of syringe agroinfiltration. The pCAMBIA1301 was transformed into Nicotiana benthamiana leaves for investigation. The effects of 5-azacytidine (AzaC), Ascorbate acid (ASC) and Tween-20 on transformation were studied. The β-glucuronidase (GUS) expression and GUS activity were respectively measured to determine the transformation efficiency. AzaC, ASC and Tween-20 all significantly affected the transformation efficiency of agroinfiltration, and the optimal concentrations of AzaC, ASC and Tween-20 for the transgene expression were identified. Our results showed that 20 µM AzaC, 0.56 mM ASC and 0.03% (v/v) Tween-20 is the optimal concentration that could significantly improve the transformation efficiency of agroinfiltration.
    [Show full text]
  • Highly Efficient Protoplast Isolation and Transient Expression System
    International Journal of Molecular Sciences Article Highly Efficient Protoplast Isolation and Transient Expression System for Functional Characterization of Flowering Related Genes in Cymbidium Orchids Rui Ren 1 , Jie Gao 1, Chuqiao Lu 1, Yonglu Wei 1, Jianpeng Jin 1, Sek-Man Wong 2,3,4, Genfa Zhu 1,* and Fengxi Yang 1,* 1 Guangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; [email protected] (R.R.); [email protected] (J.G.); [email protected] (C.L.); [email protected] (Y.W.); [email protected] (J.J.) 2 Department of Biological Sciences, National University of Singapore (NUS), 14 Science Drive 4, Singapore 117543, Singapore; [email protected] 3 National University of Singapore Suzhou Research Institute (NUSRI), Suzhou Industrial Park, Suzhou 215000, China 4 Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore 117604, Singapore * Correspondence: [email protected] (G.Z.); [email protected] (F.Y.) Received: 28 February 2020; Accepted: 24 March 2020; Published: 25 March 2020 Abstract: Protoplast systems have been proven powerful tools in modern plant biology. However, successful preparation of abundant viable protoplasts remains a challenge for Cymbidium orchids. Herein, we established an efficient protoplast isolation protocol from orchid petals through optimization of enzymatic conditions. It requires optimal D-mannitol concentration (0.5 M), enzyme concentration (1.2 % (w/v) cellulose and 0.6 % (w/v) macerozyme) and digestion time (6 h). With this protocol, the highest yield (3.50 107/g fresh weight of orchid tissue) and viability (94.21%) of × protoplasts were obtained from flower petals of Cymbidium.
    [Show full text]
  • Grapevine Protoplasts As a Transient Expression System for Comparison
    Grapevine Protoplasts as a Transient Expression System for Comparison of Stilbene Synthase Genes Containing cGMP-Responsive Promoter Elements Ilka Brehm, Regina Preisig-Müller and Helmut Kindi Fachbereich Chemie der Philipps-Universität, D-35032 Marburg, Germany Z. Naturforsch. 54c, 220-229 (1999) received December 15, 1998/January 7, 1999 cGMP, Grapevine Protoplasts, Pine, Promoter, Elicitor-Responsive, Stilbene Synthase Gene, Vitis A method for preparing elicitor-responsive protoplasts from grapevine cells kept in suspen­ sion culture was established. The protoplasts were employed in order to perform transient gene expression experiments produced by externally added plasmids. Using the gene coding for bacterial ß-glucuronidase as the reporter gene, the transient expression under the control of various promoters of stilbene synthase genes were analyzed. The elicitor-responsiveness of promoters from grapevine genes and heterologous promoters were assayed: the grapevine stilbene synthase gene VST-1 and pine stilbene synthase genes PST-1, PST-2 and PST-3. Compared to the expression effected by the cauliflower mosaic virus 35S RNA-promoter, the stilbene synthase promoters caused a 2-5-fold increase in GUS-activity. Incubation of transformed protoplasts with fungal cell wall further stimulated the stilbene synthase promot­ ers but not the 35S RNA-promoter. An even more pronounced differentiation between the promoters was observed when cGMP was included in the transient expression assays. Instead of treating transformed protoplasts with fungal cell wall we administered simultaneously cGMP and the plasmid to be tested. The cGMP-responsive increase was (a) specific concern­ ing the nucleotide applied, (b) characteristic of grapevine protoplasts, and (c) not seen with shortened promoter-GUS constructs or GUS under the control of the 35S RNA-promoter.
    [Show full text]
  • A Heritable Switch in Carbon Source Utilization Driven by an Unusual Yeast Prion
    Downloaded from genesdev.cshlp.org on September 27, 2021 - Published by Cold Spring Harbor Laboratory Press A heritable switch in carbon source utilization driven by an unusual yeast prion Jessica C.S. Brown1 and Susan Lindquist1,2,3 1Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA; 2Howard Hughes Medical Institute and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA Several well-characterized fungal proteins act as prions, proteins capable of multiple conformations, each with different activities, at least one of which is self-propagating. Through such self-propagating changes in function, yeast prions act as protein-based elements of phenotypic inheritance. We report a prion that makes cells resistant to the glucose-associated repression of alternative carbon sources, [GAR+] (for ‘‘resistant to glucose-associated repression,’’ with capital letters indicating dominance and brackets indicating its non-Mendelian character). [GAR+] appears spontaneously at a high rate and is transmissible by non-Mendelian, cytoplasmic inheritance. Several lines of evidence suggest that the prion state involves a complex between a small fraction of the cellular complement of Pma1, the major plasma membrane proton pump, and Std1, a much lower-abundance protein that participates in glucose signaling. The Pma1 proteins from closely related Saccharomyces species are also associated with the appearance of [GAR+]. This allowed us to confirm the relationship between Pma1, Std1, and [GAR+] by establishing that these proteins can create a transmission barrier for prion propagation and induction in Saccharomyces cerevisiae. The fact that yeast cells employ a prion-based mechanism for heritably switching between distinct carbon source utilization strategies, and employ the plasma membrane proton pump to do so, expands the biological framework in which self-propagating protein-based elements of inheritance operate.
    [Show full text]
  • Glycans in Plants
    Transient co-expression for fast and high-yield production of antibodies with human-like N -glycans in plants Louis-Philippe Vézina, Loic Faye, Patrice Lerouge, Marc-André d’Aoust, Estelle Marquet-Blouin, Carole Burel, Pierre-Olivier Lavoie, Muriel Bardor, Veronique Gomord To cite this version: Louis-Philippe Vézina, Loic Faye, Patrice Lerouge, Marc-André d’Aoust, Estelle Marquet-Blouin, et al.. Transient co-expression for fast and high-yield production of antibodies with human-like N -glycans in plants. Plant Biotechnology Journal, Wiley, 2009, 7 (5), pp.442-455. 10.1111/j.1467- 7652.2009.00414.x. hal-02128727 HAL Id: hal-02128727 https://hal-normandie-univ.archives-ouvertes.fr/hal-02128727 Submitted on 14 May 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Plant Biotechnology Journal (2009) 7, pp. 442–455 doi: 10.1111/j.1467-7652.2009.00414.x TransientLouis-P.BlackwellOxford,PBIPlant1467-76441467-7652©XXXOriginal 2009 Biotechnology UKArticleBlackwell VézinaPublishing expression Publishinget JournalLtd al. of humanizedLtd plantibodies co-expression
    [Show full text]
  • Transient Gene Expression Following DNA Transfer to Plant Cells: the Phenomenon; Its Causes and Some Applications
    Transient Gene Expression Following DNA Transfer to Plant Cells: The Phenomenon; Its Causes and Some Applications. A thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy in Cellular and Molecular Biology in the University of Canterbury. RICHARD JOHN WELD 2000 1 ..... ACKNOWLEDGEMENTS ..... I would like to thank Dr Jack Heinemann, Dr Colin Eady and Dr Sandra Jackson for their supervision of this project, for their criticism and their encouragement. I would also like to thank Dr Ross Bicknell for his advice and support. I acknowledge the generosity of Dr Jim Haseloff for the gift of plasmid pBINmgfp5-ER, Dr Ed Morgan for the gift of Nicotiana plumbaginifolia suspension cells and advice on their culture, Dr Steve Scofield for the gift ofpSLJll 01, Dr Nicole Houba-Herin for the gift ofpNT103 andpNT804, Dr Jerzy Paszkowski for the gift ofpMDSBAR, Dr Andrew Gleave for the gift ofpART8 and pART7, Dr n Yoder for the gift ofpAL144 and Dr Bernie Carroll for information on the construction of pSLJ3 621. This project was made possible by funds provided by a FfRST Doctoral Fellowship provided through the New' Zealand Institute for Crop and Food Research and a University of Canterbury Doctoral Scholarship. I would like to offer my grateful thanks to all those staff and students of the Plant and Microbial Sciences Department and those staff and students at the New Zealand Institute for Crop and Food Research at Lincoln who have assisted me in various ways during the course of my research and to those who have offered their friendship, support and encouragement.
    [Show full text]
  • XXI Fungal Genetics Conference Abstracts
    Fungal Genetics Reports Volume 48 Article 17 XXI Fungal Genetics Conference Abstracts Fungal Genetics Conference Follow this and additional works at: https://newprairiepress.org/fgr This work is licensed under a Creative Commons Attribution-Share Alike 4.0 License. Recommended Citation Fungal Genetics Conference. (2001) "XXI Fungal Genetics Conference Abstracts," Fungal Genetics Reports: Vol. 48, Article 17. https://doi.org/10.4148/1941-4765.1182 This Supplementary Material is brought to you for free and open access by New Prairie Press. It has been accepted for inclusion in Fungal Genetics Reports by an authorized administrator of New Prairie Press. For more information, please contact [email protected]. XXI Fungal Genetics Conference Abstracts Abstract XXI Fungal Genetics Conference Abstracts This supplementary material is available in Fungal Genetics Reports: https://newprairiepress.org/fgr/vol48/iss1/17 : XXI Fungal Genetics Conference Abstracts XXI Fungal Genetics Conference Abstracts Plenary sessions Cell Biology (1-87) Population and Evolutionary Biology (88-124) Genomics and Proteomics (125-179) Industrial Biology and Biotechnology (180-214) Host-Parasite Interactions (215-295) Gene Regulation (296-385) Developmental Biology (386-457) Biochemistry and Secondary Metabolism(458-492) Unclassified(493-502) Index to Abstracts Abstracts may be cited as "Fungal Genetics Newsletter 48S:abstract number" Plenary Abstracts COMPARATIVE AND FUNCTIONAL GENOMICS FUNGAL-HOST INTERACTIONS CELL BIOLOGY GENOME STRUCTURE AND MAINTENANCE COMPARATIVE AND FUNCTIONAL GENOMICS Genome reconstruction and gene expression for the rice blast fungus, Magnaporthe grisea. Ralph A. Dean. Fungal Genomics Laboratory, NC State University, Raleigh NC 27695 Rice blast disease, caused by Magnaporthe grisea, is one of the most devastating threats to food security worldwide.
    [Show full text]
  • Frontiers in the Standardization of the Plant Platform for High Scale Production of Vaccines
    plants Review Frontiers in the Standardization of the Plant Platform for High Scale Production of Vaccines Francesco Citiulo 1 , Cristina Crosatti 2 , Luigi Cattivelli 2 and Chiara Biselli 3,* 1 GSK Vaccines Institute for Global Health, Via Fiorentina 1, 53100 Siena, Italy; [email protected] 2 Council for Agricultural Research and Economics, Research Centre for Genomics and Bioinformatics, Via San Protaso 302, 29017 Fiorenzuola d’Arda, Italy; [email protected] (C.C.); [email protected] (L.C.) 3 Council for Agricultural Research and Economics, Research Centre for Viticulture and Enology, Viale Santa Margherita 80, 52100 Arezzo, Italy * Correspondence: [email protected]; Tel.: +39-0575-353021 Abstract: The recent COVID-19 pandemic has highlighted the value of technologies that allow a fast setup and production of biopharmaceuticals in emergency situations. The plant factory system can provide a fast response to epidemics/pandemics. Thanks to their scalability and genome plasticity, plants represent advantageous platforms to produce vaccines. Plant systems imply less complicated production processes and quality controls with respect to mammalian and bacterial cells. The expression of vaccines in plants is based on transient or stable transformation systems and the recent progresses in genome editing techniques, based on the CRISPR/Cas method, allow the manipulation of DNA in an efficient, fast, and easy way by introducing specific modifications in specific sites of a genome. Nonetheless, CRISPR/Cas is far away from being fully exploited for vaccine expression in plants. In this review, an overview of the potential conjugation of the renewed vaccine technologies (i.e., virus-like particles—VLPs, and industrialization of the production process) Citation: Citiulo, F.; Crosatti, C.; with genome editing to produce vaccines in plants is reported, illustrating the potential advantages in Cattivelli, L.; Biselli, C.
    [Show full text]