Biology of Blood and Marrow Transplantation 10:40-48 (2004) ᮊ 2004 American Society for Blood and Marrow Transplantation 1083-8791/04/1001-0004$30.00/0 doi:10.1016/j.bbmt.2003.09.013 Allogeneic versus Syngeneic Killer Splenocytes as Effector Cells for the Induction of Graft-versus- Tumor Effect Shoshana Morecki, Elena Yacovlev, Yael Gelfand, Anna Vilensky, Shimon Slavin Department of Bone Marrow Transplantation & Cancer Immunotherapy, Cell Therapy & Transplantation Research Center, Hadassah University Hospital, Jerusalem, Israel Correspondence and reprint requests: Shoshana Morecki, PhD, Department of BMT, Hadassah University Hospital, Jerusalem 91120, Israel (e-mail:
[email protected]). Received August 8, 2003; accepted September 22, 2003 ABSTRACT The effect of allogeneic versus syngeneic killer cells derived from normal or severe combined immunodefi- ciency disease (SCID) mice was evaluated for induction of antitumor reaction in a murine model of mammary carcinoma. Tumor cells of H-2d origin were injected intravenously into H-2d/b mice 24 hours after total body irradiation (4 Gy). On the following day, lymphokine-activated killer (LAK) splenocytes, derived from either minor (H-2d) or major (H-2b) histocompatibility complex (MHC)–mismatched parental normal mice or MHC (H-2b)–mismatched SCID mice, were given intravenously. LAK cells of H-2d normal or SCID mice, syngeneic to the tumor, were inoculated in parallel. The results show that LAK cells derived from minor histocompat- ibility complex–mismatched or MHC-mismatched parental normal mice improved the probability of tumor- free survival as compared with LAK cells syngeneic to the tumor cells, but they aggravated the severity of graft-versus-host disease.