List and Description of Genes Relevant to This Proposal

GENE Stock Allele Gene Full Accession Number Product Name Description Citation Symbol number symbol Name TAIR GenBank ADP GLUCOSE adg1-1 Gene:3356178 PYROPHOSP ADG1 CS3094 U72351 [WLY+98] (Col) HORYLASE SMALL SUBUNIT Floral homeotic gene encoding a MADS domain . ag-1 AGAMOUS AG CS25 AGAMOUS Gene:1944469 Specifies floral meristem [PNF96] (Ler) PROTEIN and carpel and stamen identity. Binds CArG box sequences. Floral homeotic gene encoding a MADS domain APETALA protein homologous to SRF 1(FLORAL transcription factors. AP1 ap1-1 CS28 HOMEOTIC Gene:1944474 Z16421 AGL7 Specifies floral meristem [IS90] (AGL7) (Ler) PROTEIN and sepal identity. Required APETALA 1) for the transcriptional activation of AGAMOUS. Interacts with LEAFY. Floral homeotic gene containing an AP2-type FLORAL DNA binding domain. AP2 ap2-9 HOMEOTIC CS3084 APETALA 2 Gene:1944475 U12546 Involved in establishing [BSM89] (FLO2) (Ler) PROTEIN floral meristem and petal APETALA 2 identity and in the control of seed development.

GENE Stock Allele Gene Full Accession Number Product Name Description Citation Symbol number symbol Name TAIR GenBank Floral homeotic gene encoding a MADS domain FLORAL protein homologous to SRF ap3-1 HOMEOTIC AP3 CS3085 APETELA3 Gene:1944476 M86357 transcription factors. [BSM89] (Ler) PROTEIN Specifies petal and stamen APETALA 3 identities. Associates with PISTILLATA. Floral homeotic gene encoding a MADS domain cal1-1, CAL CAULIFLO protein homologous to AP1. CS6161 ap1-1 Gene:1944493 L36925 [KSY95] (AGL10) WER Enhances the flower to (Ws/Ler) shoot transformation in ap1 mutants. MYB- CIRCADIAN RELATED CLOCK DNA binding protein CCA1 Gene:2005483 U28422 DNA [WKS+97] ASSOCIATE BINDING D 1 PROTEIN Similar to the product of the Polycomb-group gene clf-2 CURLY Enhancer of zeste. Required CLF CS8853 Gene:2005466 CLF [GPM+97] (Ler) LEAF for stable repression of AG and AP3. Putative role in cell fate determination. promotes flowering and co-6 encodes a protein showing CO CS179 CONSTANS Gene:1944846 L81120 [PRL+95] (Ler) similarities to transcription factors Putative transcription factor with zinc finger and helix- CRABS TRANSCRIPT loop-helix domains, the later crc-1 CRC CS3814 CLAW Gene:1944864 AF132606 ION FACTOR similar to HMG boxes. [BS99] (Ler) (YABBIE) CRC Involved in specifying abaxial cell fate in the carpel.

GENE Stock Allele Gene Full Accession Number Product Name Description Citation Symbol number symbol Name TAIR GenBank CRYPTOCH ROME 1 CRY1: CRYPTOCHR CRY1 hy4-1 ELONGATE gene:1944514 OME 1 Flavin-type blue-light CS70 [AC93] (HY4) (Ler-0) D HY4: APOPROTEI photoreceptor. HYPOCOTY gene:1944565 N L 4 Blue light CRYPTOCH CRYPTOCHR mediating blue-light ROME 2 OME 2 regulated cotyledon fha-1 PHOTOREC CRY2: CRY2 CS108 U43397 APOPROTEI expansion and flowering [LACC96] (Ler) EPTOR/PHO gene:1944515 N time. Positive regulator of TOLYASE the flowering-time gene HOMOLOG CONSTANS. EARLY AF410337 elf3-7 At2g25920/F1 ELF3 CS3793 FLOWERIN Gene:1944909 (look for [ZSJM92] (Col ) 7H15.5 G 3 At2g25920) EMBRYONI Involved in regulating EMF1 C FLOWER Gene:1945087 [YCS95] reproductive development 1 FLOWERIN FLOWERING Involved in the promotion of G TIME TIME the transition of the CONTROL CONTROL vegetative meristem to PROTEIN fca-6 PROTEIN reproductive development. FCA CS172 FCA Gene:1944544 Z82991 [FAPP96] (Ler) FCA ALPHA, Four forms of the protein ALPHA, BETA, (alpha, beta, delta and BETA, DELTA AND gamma) are produced by DELTA AND GAMMA alternative splicing. GAMMA FKF1: LKP1:AB0 Gene:3353704 38796 LKP1: FKF1 LOV KELCH FKF1:AF21 Gene:3356321 FKF1 (LKP1, PROTEIN 1 6523 (Landsberg erecta) [NLR+00] FKF1: ZTL) FKF1-like Gene:3356319 protein 2: (FKF1-LIKE AF216525 PROTEIN 2)

GENE Stock Allele Gene Full Accession Number Product Name Description Citation Symbol number symbol Name TAIR GenBank FLC-Col FLOWERIN FLC CS189 Gene:1945110 [SA96] (Ler/Col) G LOCUS C fpa-2 FPA CS166 Gene:1945114 [KHV91] (Ler) FRIGIDA FRI FRI-Sf2 CS6209 FLOWERIN Gene:1945115 AF228500 [CD94] (FLA) (Col) G LOCUS A FT, together with LFY, promotes flowering and is ft-3 FLOWERIN antagonistic with its FT CS185 Gene:1944549 AB027504 [KKG+99] (Ler-0) G LOCUS T homologous gene, TERMINAL FLOWER1 (TFL1) AGAMOUS- FUL agl8-1 contains MADS box and K CS3759 LIKE 8 or Gene:1944676 U33473 AGL8 [MY95] (AGL8) (Ler) domains FRUITFULL fve-2 FVE CS174 Gene:1945130 [KHV91] (Ler) homeodomain- fwa-2 (Ler- AF243535 containing FWA CS188 Gene:1945131 homeodomain-containing [SJL+00] 0) transcription transcription factor FWA factor FWA Catalizes the conversion of ENT- geranylgeranyl GA ga1-10 KAURENE pyrophosphate (GGPP) to GA1 CS3825 REQUIRING Gene:1944553 U11034 [SK94] (Ler) SYNTHETAS copalyl pyrophosphate 1 E A (CPP) of gibberellin biosynthesis Catalyzes the second step in GA ENT- the cyclization of GGPP to ga2-1 Gene:2005472 GA2 CS59 REQUIRING AF034774 KAURENE ent-kaurene in the [YKK98] (Ler) 2 SYNTHASE gibberellins biosynthetic pathway. GA ENT- Involved in later steps of the ga3-1 GA3 CS60 REQUIRING Gene:1944555 AF047719 KAURENE gibberellins biosynthetic [HSO+98] (Ler) 3 OXIDASE pathway.

GENE Stock Allele Gene Full Accession Number Product Name Description Citation Symbol number symbol Name TAIR GenBank GA REQUIRING 4 Involved in later steps of the ga4-1 GA4 CS61 (GIBBEREL Gene:1944556 L37126 GA4 gibberellins biosynthetic [CHG95] (Ler) LIN 3 BETA- pathway. HYDROXYL ASE) GA GIBBERELLI Involved in later steps of the ga5-1 GA5 CS62 REQUIRING Gene:1944557 N 20- gibberellins biosynthetic [XLW+95] (Ler) 5 OXIDASE pathway. Similar to a putative GA gai-1 GAI transcription factor and GAI CS63 INSENSITIV Gene:1944558 [PCR+97] (Ler) PROTEIN transcriptional coactivators. E Repressor of GA responses. gi-6 late flowering protein; GI CS183 GIGANTEA Gene:1944560 AF076686 GI [PSK+99] (Ler) photoperiod-related protein Arabidopsis Member of the floral thaliana floral homeotic AGAMOUS ENHANCER HUA1 Gene:1945163 AY024357 homeotic pathway. [LJC01] OF AG-4 1 protein HUA1 nuclear CCCH zinc finger (HUA1) protein putative Putative transcription factor. ENHANCER Gene:1944563 transcription Member of the floral HUA2 AF116556 [CM99] OF AG-4 2 factor, homeotic AGAMOUS HUA2 pathway. LUMINIDEP ENDENS Arabidopsis Arabidopsis thaliana thaliana gld LD CS8012 Wassilewskij Gene:1945130 U03456 Wassilewskija [LAG+94] (Col) a Luminidepend Luminidepen ens dens mRNA, complete cds.

GENE Stock Allele Gene Full Accession Number Product Name Description Citation Symbol number symbol Name TAIR GenBank Together with AP1, LFY lfy-11 promotes the transition from LFY CS6280 LEAFY Gene:1944572 LEAFY [SIC96] (Ler) inflorescence to floral meristem MYB Arabidopsis thaliana mRNA late elongated transcription for LATE ELONGATED LHY hypocotyl AJ006404 [SRS+98] factor HYPOCOTYL MYB

transcription factor. Arabidopsis LUG lug-1 thaliana TUP1-like; contains WD CS8031 Gene:3355513 AF277458 LEUNIG [CL00] (LEUNIG) (Ler) LEUNIG repeats (LEUNIG) Arabidopsis NAP thaliana nap AJ222713 [SM98] gene PHOSPHOG PHOSPHOGL pgm-1 PGM CS210 LUCOMUTA Gene:1945261 AF216580 UCOMUTAS [CLB98] (Col) SE E Blue light receptor CRYPTOCH PHH1: CRYPTOCHR mediating blue-light ROME 2 gene:1944592 PHH1 OME 2 regulated cotyledon PHOTOREC FHA: (FHA, U62549 APOPROTEI expansion and flowering [HBH96] EPTOR/PHO gene:1944545 CRY2) N time. Positive regulator of TOLYASE CRY2: the flowering-time gene HOMOLOG gene:1944515 CONSTANS. The sole photoreceptor mediating the FR high PHYTOCHR irradiance response (HIR). PHYA OME A Major regulator in red-light (FHY2, phyA-201 ELONGATE PHYTOCHRO induction of phototropic CS6219 Gene:1944593 AC003970 [SQ89] FRE1, (Ler) D ME A enhancement. Involved in HY8) HYPOCOTY the regulation of de- L 8 etiolation. Exists in two inter-convertible forms: Pr and Pfr (active).

GENE Stock Allele Gene Full Accession Number Product Name Description Citation Symbol number symbol Name TAIR GenBank Red/far-red photoreceptor involved in the regulation of Arabidopsis Phyb: de-etiolation. Exists in two PHYTOCHRO PHYB phyB-1 thaliana phyB gene:1944594 inter-convertible forms: Pr CS6211 X17342 ME B [RNP+93] (HY3) (Ler) mRNA for HY3: and Pfr (active). Involved in

phytochrome. gene:1945166 the light-promotion of seed germination and in the shade avoidance response. FLORAL HOMEOTIC pid-1 PI CS8062 PISTILLATA Gene:1944595 D30807 PROTEIN [WJP+93] (Ler) PISTILLATA

rga-2, ga1- REPRESSOR RGA CS3742 3 Gene:1945306 [SCS98] OF GA1-3 (Ler) STRESS Arabidopsis thaliana stress SEP1 ENHANCED Gene:3355554 AF133716 SEP1 enhanced protein 1 [HA00] (AGL2) PROTEIN 1 STRESS SEP2 Arabidopsis thaliana stress ENHANCED Gene:3354073 AF133717 SEP2 [HA00] (AGL4) enhanced protein 2 PROTEIN 2 SEP3 SEPALLAT Arabidopsis thaliana Gene:1944470 AF015552 SEP3 [PDB+00] (AGL9) A3 MADS-box (AGL9) mRNA SEX1 sex1-1 STARCH Required for starch (SOP, CS3093 Gene:1945356 AF312027 SEX1 [CLB98] (Col) EXCESS 1 degradation. SOP1) Arabidopsis thaliana SHP1 shp1-1 transcription CS3841 transcription Gene:3355036 M55550 [YM91] (AGL1) (Ler) factor factor (AGL1), Arabidopsis thaliana SHP2 shp2-1 transcription CS3845 transcription Gene:3354358 M55553 [YM91] (AGL5) (Ler) factor factor (AGL5)

GENE Stock Allele Gene Full Accession Number Product Name Description Citation Symbol number symbol Name TAIR GenBank MADS-BOX SOC1 AGAMOUS- Gene:1944636 AY007726 PROTEIN [BKC+00] (AGL20) LIKE 20 AGL20 SPT CS275 spt-2 SPATULA Gene:1945376 AF319540 [HAB+01] Contains a tetratricopeptide repeat region, and a novel carboxy-terminal region. Mutations in both domains spy-5 increase GA signal SPY CS8094 SPINDLY Gene:1944614 U62135 SPINDLY [JO93] (Ler) transduction. Putative N- acetyl glucosamine transferase that may glycosylate other molecules involved in GA signaling. Controls inflorescence meristem identity. Involved tfl1-2 in the floral initiation CS3091 (Ler) TERMINAL TERMINAL TFL1 Gene:1944617 D86932 process. Ortholog of the [BRV+97] CS6235 tfl1-11 FLOWER1 FLOWER 1 Antirrhinum gene (Col) CENTRORADIALIS (CEN). TIMING OF toc1 CAB TOC1 CS3756 Gene:1945416 AF272039 [MCS+95] (C24) EXPRESSIO N 1 Required for the proper identity of the floral meristem. Involved in establishing the whorled UNUSUAL ufo-2 pattern of floral organs, in UFO CS6294 FLORAL Gene:1944627 UFO [LM95] (Col) the control of specification ORGANS of the floral meristem, and in the activation of APETALA3 and PISTILLATA. Vernalization VRN2 Gene:1945456 [CWD96] 2

GENE Stock Allele Gene Full Accession Number Product Name Description Citation Symbol number symbol Name TAIR GenBank ZTL ZTL:AF254 (LKP1, ZEITLUPE Gene:3356323 ZTL [SSMK00] 413 FKF1)