Product Datasheet

RFC4 Antibody NB100-233

Unit Size: 100 ul Store at 4C. Do not freeze.

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Updated 2/7/2017 v.20.1

Earn rewards for product reviews and publications. Submit a publication at www.novusbio.com/publications Submit a review at www.novusbio.com/reviews/destination/NB100-233 Page 1 of 7 v.20.1 Updated 2/7/2017 NB100-233 RFC4 Antibody Product Information Unit Size 100 ul Concentration 1.0 mg/ml Storage Store at 4C. Do not freeze. Clonality Polyclonal Preservative 0.09% Sodium Azide Isotype IgG Purity Immunogen affinity purified Buffer Tris-Citrate/Phosphate (pH 7.0 - 8.0) Target Molecular Weight 40 kDa Product Description Host Goat ID 5984 Gene Symbol RFC4 Species Human Reactivity Notes Human Immunogen The immunogen recognized by this antibody maps to a region between residues 300 and 350 of human subunit 4 using the numbering given in entry NP_002907.1 (GeneID 5984). Product Application Details Applications Western Blot, Proximity Ligation Assay Recommended Dilutions Western Blot 1:500-1:5000, Proximity Ligation Assay 1:1000-1:20000 Application Notes The observed molecular weight of the may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Images Western Blot: RFC4 Antibody [NB100-233] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC4 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Page 2 of 7 v.20.1 Updated 2/7/2017 Western Blot: RFC4 Antibody [NB100-233]

Western Blot: RFC4 Antibody [NB100-233] - Nuclear extract (50 ug) from HeLa cells. Antibody used at 1 ug/ml.

Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100- 233) and rabbit anti-human BRD4 (NBP1-18874). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human RFC4 (NB100-233) and rabbit anti-human ATAD5 (NB100-57495). Image merged from DAPI (2ms), FITC/Green (25ms) and TRITC/orange (250ms) exposures, 40X magnficiation. Page 3 of 7 v.20.1 Updated 2/7/2017 Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anit-human RFC4 (NB100- 233) and rabbit anti-human BRCA1 (NB100-61617). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100- 233) and rabbit anti-human CTF18 (NBP1-03346). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100- 233) and rabbit anti-human HDAC1 (NB100-2241). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100- 233) and rabbit anti-human PCNA (NB100-456). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification. Page 4 of 7 v.20.1 Updated 2/7/2017 Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100- 233) and rabbit anti-human PML (NB100-59787). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100- 233) and rabbit anti-human RFC3 (NB100-435). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100- 233) and rabbit anti-human RFC4 (NB100-403). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100- 233) and rabbit anti-human RPA2 (NB100-332). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification. Page 5 of 7 v.20.1 Updated 2/7/2017 Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100- 233) and rabbit anti-human phospho-RPA2 [S33] (NB100-544). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100- 233) and rabbit anti-human phospho-RPA2 [S4/S8] (NBP1-23017). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification.

Proximity Ligation Assay: RFC4 Antibody [NB100-233] - Secondary- conjugate Duolink II PLA in Hela cells. goat anti-human RFC4 (NB100- 233) and rabbit anti-human RuvBL2 (NBP1-40354). Image merged from DAPI (2ms) and Texas Red (200ms) exposures, 40X magnification. Novus Biologicals USA Novus Biologicals Canada 8100 Southpark Way, A-8 461 North Service Road West, Unit B37 Littleton, CO 80120 Oakville, ON L6M 2V5 USA Canada Phone: 303.730.1950 Phone: 905.827.6400 Toll Free: 1.888.506.6887 Toll Free: 855.668.8722 Fax: 303.730.1966 Fax: 905.827.6402 [email protected] [email protected]

Novus Biologicals Europe General Contact Information 19 Barton Lane www.novusbio.com Abingdon Science Park Technical Support: [email protected] Abingdon, OX14 3NB, United Kingdom Orders: [email protected] Phone: (44) (0) 1235 529449 General: [email protected] )UHH3KRQH0800 37 34 15 Fax: (44) (0) 1235 533420 [email protected]

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Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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