Product Information Sheet for NR-20723

Monoclonal Anti-Human NLRP12 (NALP12), be stored at -20°C or colder immediately upon arrival. Freeze-thaw cycles should be avoided. Clone U54.M.hNALP12.14 (produced in vitro) Functional Activity: NR-20723 reacts with human NLRP12 in ELISA and western Catalog No. NR-20723 blot assays. See Certificate of Analysis for details. The 1 antibody is also reported to function in immunoprecipitation. For research use only. Not for human use. Citation:

Acknowledgment for publications should read “The following Contributor: reagent was obtained through BEI Resources, NIAID, NIH: Richard J. Ulevitch, Ph.D., and Luc Teyton, M.D., Ph.D., Monoclonal Anti-Human NLRP12 (NALP12), Clone Department of Immunology and Microbial Science, The U54.M.hNALP12.14 (produced in vitro), NR-20723.” Scripps Research Institute, La Jolla, CA, USA`

Biosafety Level: 1 Manufacturer: Appropriate safety procedures should always be used with BEI Resources this material. Laboratory safety is discussed in the following

publication: U.S. Department of Health and Human Services, Product Description: Public Health Service, Centers for Disease Control and Antibody Class: IgG2aκ Prevention, and National Institutes of Health. Biosafety in Mouse monoclonal antibody prepared against the human Microbiological and Biomedical Laboratories. 5th ed. NLRP12 protein (also known as NALP12) was purified from Washington, DC: U.S. Government Printing Office, 2009; see clone U54.M.hNALP12.14 hybridoma supernatant by protein www.cdc.gov/biosafety/publications/bmbl5/index.htm. G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63Ag8.653 mouse myeloma Disclaimers: cells with splenocytes from mice immunized by You are authorized to use this product for research use only. intraperitoneal injection with a truncated recombinant form of 1 It is not intended for human use. the human NLRP12 protein containing the pyrin domain.

Use of this product is subject to the terms and conditions of NLRP12 is encoded by the NLRP2 (nucleotide-binding the BEI Resources Material Transfer Agreement (MTA). The domain and leucine rich repeat containing family, pyrin MTA is available on our Web site at www.beiresources.org. domain containing 12) gene. NLRP proteins are characterized by an N-terminal pyrin domain, a central While BEI Resources uses reasonable efforts to include NTPase domain and a C-terminal leucine-rich repeat region. accurate and up-to-date information on this product sheet, NLR proteins are localized to the cytoplasm and recognize ® neither ATCC nor the U.S. Government makes any microbial products, and their activation results in warranties or representations as to its accuracy. Citations inflammatory responses mediated by NF-κB, MAP kinase, or 2 from scientific literature and patents are provided for caspase activation. Certain NLRPs are involved in the ® informational purposes only. Neither ATCC nor the U.S. formation and activation of inflammasomes, large Government warrants that such information has been cytoplasmic multiprotein complexes that in turn recruit and 3,4 confirmed to be accurate. activate caspase-1. Activated caspase-1 cleaves the pro- forms of the interleukin-1 cytokine family members leading to This product is sent with the condition that you are the secretion of active IL-1 family cytokines, which have responsible for its safe storage, handling, use and disposal. multiple pro-infammatory activities. Inflammasomes are an ® ATCC and the U.S. Government are not liable for any important component of the innate immune system. A variety damages or injuries arising from receipt and/or use of this of names (including NALP) have been used to describe the product. While reasonable effort is made to ensure products encoded by the NLR gene family. A standardized 5 authenticity and reliability of materials on deposit, the U.S. nomenclature for the family was recently proposed. ® Government, ATCC , their suppliers and contributors to BEI

Resources are not liable for damages arising from the Material Provided: misidentification or misrepresentation of products. Each vial of NR-20723 contains approximately 100 µL of purified monoclonal antibody in PBS. The concentration, Use Restrictions: expressed as mg per mL, is shown on the Certificate of This material is distributed for internal research, non- Analysis. commercial purposes only. This material, its product or its

derivatives may not be distributed to third parties. Except as Packaging/Storage: performed under a U.S. Government contract, individuals NR-20723 was packaged aseptically in screw-capped plastic contemplating commercial use of the material, its products or vials and is provided frozen on dry ice. The product should

BEI Resources E-mail: [email protected] www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898

Product Information Sheet for NR-20723

its derivatives must contact the contributor to determine if a license is required. U.S. Government contractors may need a license before first commercial sale.

References: 1. R. J. Ulevitch and L. Teyton, personal communication. 2. Wilmanski, J. M., T. Petnicki-Ocwieja, and K. S. Kobayashi. “NLR Proteins: Integral Members of Innate Immunity and Mediators of Inflammatory Diseases.” J. Leukoc. Biol. 83 (2008): 13-30. PubMed: 17875812. 3. Latz, E. “The Inflammasomes: Mechanisms of Activation and Function.” Curr. Opin. Immunol. 22 (2010): 28-33. PubMed: 20060699. 4. Skeldon, A., and M. Saleh. “The Inflammasomes: Molecular Effectors of Host Resistance Against Bacterial, Viral, Parasitic, and Fungal Infections.” Front. Microbiol. 2 (2011): 15. PubMed: 21716947. 5. Ting, J. P., et al. “The NLR Gene Family: A Standard Nomenclature.” Immunity 28 (2008): 285-287. PubMed: 18341998.

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BEI Resources E-mail: [email protected] www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898