I.V. ZMITROVICH1, V.F. MALYSHEVA1, W.A. SPIRIN2 1 V.L. Komarov Botanical Institute, Russian Academy of Sciences 2 Popov St., St. Petersburg, 197376, Russia [email protected]; [email protected] 2 University of the Humanities 15 Fuchika St., St. Petersburg, 192238, Russia [email protected] A NEW SPECIES (, ) FROM ZHIGULI, EUROPEAN RUSSIA

Key words: Basidiomycetes, Polyporales, broad-leaf forests, Pachykytospora wasseri, new species The Pachykytospora Kotl. et Pouzar [4] unites some prostrate pore fungi having -like skeleto-binding hyphae and uneven ellipsoid basidiospores, which are characterized by rather longitudinal arrangements of the crests. The last feature as well as the basidiocarp resupinate trait distinctly mark the differences between the genus Pachykytospora from the closely related , albeit some authors [1, 6] do not make these distinctions between the genera. According to many reasonable yet narrow concepts, the genus Pachykytospora includes nine species [1, 2, 5, 7] having south-temperate to tropical distribution. All of them are clearly distinguishable according to spore sizes, dextrinoid reaction of skeletals, anatomy of dissepiment edge, and some features of pore surface. Being a key nemoral refugium, Zhiguli broadleaved forests (Samara Region, Russia) reveal many floristic novelties. In particular, there is an unusual with sufficient Pachykytospora characteristics described here. This fungus is so unique that it certainly requires independent species status. Its detailed description and discussion are given below.

Pachykytospora wasseri Zmitr., V. Malysheva et Spirin sp. nov. — see Figure. Basidiomata annua, prostrata, late effusa (ad 10 cm diam.), fibrosa ad coriaceo- chartacea. Margo effusus, tenuis, sterilis ad fertilis, albidus ad flavido-ochraceus, gelatinescens. Hymenophorum tubulosum, leniter nodulosum, facies pororum albida ad cremea, deinde flavo-brunnescens, pori (1)2—4 per mm, irregulariter collocati, circulati ad subsinuosi, tenuitunicati. Subiculum 0.5—1 mm crassis, cremeum, fibrillosum. Tubuli obscure bistratosi, 2—5 mm longae, cremei ad ochracei. Systema hypharum dimiticum. Hyphae ligativo-sceletales 2—2.6 µm diam., leniter dextrinoideae cyanophiliesque. Hyphae generatoriae fibulatae, hyalinae, 1.5— 2.5 µm in diametro. Basidia 13—24 × 5—7.5 µm, clavata, tetrasporifera, basi fibulata. × µ Basidiosporae (7.8)8—9(10.4) 3.9—5.2 m [Q = (1.5)1.7—1.9(2.7); Qm = 1.9],

© I.V. ZMITROVICH, V.F. MALYSHEVA, W.A. SPIRIN, 2007

42 ISSN 0372-4123. Ukr. Botan. Journ., 2007, vol. 64, ¹ 1 Pachykytospora wasseri (Holotypus). A — basidiome outline from the pore surface (scale bar = 5 mm); B — micromorphology details: 1 — basidioles, 2 — basidiospores, 3 — skeleto-binding hyphae, 4 — aleuria (scale bar = 10 µm)

ellipsoideae, leviter truncatae, lineariter tuberculosae, leniter dextrinoideae, cyanophilae. Aleuria adsunt in trama, fusoidea ad brevicylindracea, 12—25 × 3—6 µm, guttulata. Holotypus:Russia, Samara Reg., Stavropol Dist., Zhiguli Nat. Res., Padus avium, 12.09.2006, V.F. Malysheva, E.F. Malysheva, I.V. Zmitro- vich, Isotypus — LE 214872. Basidiomata annual, prostrate, largely effused along bark (up to 10 cm in diam.), fibrous to coriaceous or creta- ceous. Margin effuse, thin, firstly sterile, white, later fertile, with lemon to ochraceous tints, slightly gelatinized. Pore surface uneven, weakly nodulose, firstly white with creamish stains, then cream with gelatinized bright-yellow (or lemon-yellow) patches; pores (1)2—4 per mm, of irregular shape, round to slightly sinuous and open in oblique positions, rather thin-walled. Section: subiculum 0.5—1 mm thick, pale cream, fibrous; tubes indistinctly two-layered (both layers are of the same season; growing layer was slightly turned due to fall of trunk), 2—5 mm thick, cream to yellowish-ochraceous, partly agglutinated. Odor absent; taste bitter (reminiscent of Oligoporus stipticus). Hyphal system dimitic. Skeleto-binding hyphae dominating in all parts of basidiocarp, thick-walled, yellowish in IKI, weakly cyanophilous, 2—2.6 µm wide, tightly interwoven in subiculum, and densely packed in tube trama. Generative hyphae clamped, hyaline, 1.5—2.5 µm wide. Basidia 13—24 × 5—7.5 µm, clavate, 4-spored, fibulate at × µ the base. Basidiospores (7.8)8—9(10.4) 3.9—5.2 m [Q = (1.5)1.7—1.9(2.7); Qm = 1.9 — see table 1], ellipsoid, indistinctly to clearly truncate, minutely rough due to tuberculate exospore with linear arrangement of tubercles, hyaline with irregular oil- drops, weakly dextrinoid, strongly cyanophilous. Aleuria generating in tube trama, fusoid to barrel-shaped, 12—25 × 3—6 µm, guttulate.

ISSN 0372-4123. Óêð. áîòàí. æóðí., 2007, ò. 64, ¹ 1 43 Table 1. Spore variability of Pachykytospora wasseri

L × WQ L× WQL× WQ 9.5 × 5.5 1.7 8 × 3.9 2.1 9.1 × 4.9 1.9 10.3 × 5.5 1.9 7.8 × 4.9 1.6 8 × 3.4 2.4 9 × 5.7 1.6 8.3 × 3.4 2.4 9.9 × 4.9 2.0 10.3 × 5.3 1.9 7.8 × 4.7 1.7 9.1 × 4.2 2.2 8.6 × 5.5 1.6 7.8 × 5.2 1.5 7.8 × 3.4 2.3 10 × 5.5 1.8 9.9 × 5.2 1.9 10.4 × 3.9 2.7 9.5 × 5.5 1.7 7.8 × 3.6 2.2 8.3 × 5.2 1.6 9 × 5.6 1.6 10.4 × 4.7 2.2 8 × 4.9 1.6 8.6 × 5.5 1.6 9.6 × 3.9 2.5 8.3 × 3.9 2.1 10.4 × 5.2 2.0 7.8 × 4.2 1.9 8 × 5.2 1.5

Note: L — spore length, W — spore wide, Q — spore quotient (Q = L / W)

Etymology. The species epithet is given in honor of the great connoisseur of agaricoid and ganodermoid fungi, Prof. Solomon P. Wasser, on his 60th birthday. Affinities. The closest relatives of newly described species are Pachykytospora tuberculosa and P. subtrametea. However, there are clear differences between mentioned species as well (table 2). P. wasseri has pores sufficiently larger than in P. subtrametea, but they are appreciably finer than the pores in P. tuberculosa (the pores exceed 1 mm wide in many cases). Similarly to P. tuberculosa, a new species is characterized by dextrinoid skeletals (albeit coloration is paler); however, its spore sizes and basidiome consistency sharply differ from those of P. tuberculosa. The spore characteristics of P. wasseri are closer to those of P. subtrametea, but the spores of P. wasseri have steadily larger spore quotients, i.e., they are longer on their longitudinal axes. Moreover, the spores of P. wasseri are slightly dextrinoid — this feature was not mentioned for P. subtrametea [5]. Discussion. The analysis of distribution of the Pachykytospora species shows a certain disjunction between European quercicolous P. tuberculosa and Asian

Table 2. Comparison of characters in species of Pachykytospora concerned

Diagnostical features P. tuberculosa P. wasseri P. subtrametea Basidiome consistency suberose coriaceous coriaceous Pores per 1 mm 0.4—2 1—4 3—5 Dextrinoid reaction of skeletals strong fine absent Spore dimensions, µm (9)10—14 × 4.6—7.0 (7.8)8—9(10.4) × (7.9)8.1—10.5(11) × × 3.9—5.2 × (4.5)5—6.3(7)

Qm 1.89 1.90 1.68 Substrata Quercus, rarely Castanea, Padus avium Padus asiatica, Salix Crataegus, Malus, Fagus sp., Tilia amurensis

44 ISSN 0372-4123. Ukr. Botan. Journ., 2007, vol. 64, ¹ 1 P. subtrametea. The only known locality of P. wasseri is near the eastern distribution limits of P. tuberculosa (southern Urals) and outside of the westernmost boundaries of the P. subtrametea areal (West Siberia). Only a little could be said on ecology of P. wasseri. Evidently, it is comparable in its preferences with its sib P. subtrametea, preferring shadowy broadleaved forests, where it occupies drying and just fallen trunks and thick branches of deciduous wood. Like other Pachykytospora species, P. wasseri is a white-rot fungus causing intensive decay of adjacent wood. Additional material examined. Pachykytospora alabamae (Berk. et Cooke) Ryvarden — H.W. Ravenel, Fungi Americani Exsiccati, Georgia, Florida, S. Carolina, LE 30759; Georgia, St. Simons Island, on hardwood shrub, 04.08.1950, J.L. Lowe, LE 210073. P. papyracea (Schwein.) Ryvarden — H.W. Ravenel, Fungi Americani Exsiccati, Georgia, Florida, S. Carolina, LE 30822. P. tuberculosa (Fr.) Kotlaba et Pouzar — Stockholm, Djurgarden, on the under- side of dead, still-attached branches of live Quercus robur, 28.01.1894, L. Romell, LE 31873; Ukraine, Crimea, «Crimsky reserve», on fallen Q. robur, 09.09.1937, A. Bondartsev, LE 31872, LE 31881; Ibid., on fallen Q. pubescens, 01.09.1956, L. Vassilieva, A. Bondartsev, LE 31885; Ukraine, Zakarpatye, on fallen Fagus sylvatica, 11.08.1956, E. Parmasto, LE 31883; Hungari, Prencow, on Quercus sp., 18.09.1897, A. Kmet, A. Bondartsev, LE 208305; Belarus, «Belovezhskaya pushcha» reserve, on trunks of Q. robur, 10.09.1966, M. Bondartseva, P. Mikhalevich, LE 31887, LE 31886; Aserbaijan, on dry branches of Tilia sp., 1949, G. Ibrahimov, A. Bondartsev, LE 31879.

1. Dai Y.C., Niemelä T., Kinnunen J. The genera Abundisporus and Perenniporia (Basidiomycota) in China, with notes on Haploporus // Ann. Bot. Fenn. — 2002. — 39. — P. 169—182. 2. David A., Rajchenberg M. West African : new species and new combinations // Mycotaxon. — 1992. — 45. — P. 131—148. 3. Hattori T., Adhikari M.K., Suda T., Doi Y. A list of polypores (Basidiomycotina, Aphyllophorales) collected in Jumla, Nepal // Bull. Nat. Sci. Mus., Tokyo. Ser. B. — 2002. — 28. — P. 27—38. 4. Kotlaba F., Pouzar Z. A new genus of the polypores — Pachykytopsora gen. nov. // Èeská Mykol. — 1963. — 17. — P. 27—34. 5. Kotlaba F., Pouzar Z. An interesting Asian polypore Pachykytospora subtrametea // Èeská Mykol. — 1979. — 33. — P. 129—133. 6. Pi¹tek M. Taxonomic position and world distribution of Pachykytospora nanospora () // Ann. Bot. Fenn. — 2005. — 42. — P. 23—25. 7. Ryvarden L. A critical checklist of the Polyporaceae in tropical East Africa // Norw. J. Bot. — 1972. — 19. — P. 229—238. Recomended for publication Submitted 06.11.2006 By A.S. Buchalo

ISSN 0372-4123. Óêð. áîòàí. æóðí., 2007, ò. 64, ¹ 1 45 ².Â. Çìèòðîâè÷1, Â.Ô. Ìàëèøåâà1, Â.À. Ñï³ð³í2 1 Áîòàí³÷íèé ³íñòèòóò ³ì. Â.Ë. Êîìàðîâà ÐÀÍ, ì. Ñàíêò-Ïåòåðáóðã 2 Ñàíêò-Ïåòåðáóðçüêèé Ãóìàí³òàðíèé óí³âåðñèòåò ïðîôñï³ëîê

ÍÎÂÈÉ ÂÈÄ ÐÎÄÓ PACHYKYTOSPORA ( BASIDIOMYCOTA, POLYPORALES) ²Ç ÆÈÃÓ˲ (ªÂÐÎÏÅÉÑÜÊÀ ÐÎѲß)

Îïèñàíî íîâèé âèä ðîäó Pachykytospora — P. wasseri, çíàéäåíèé ó ò³íüîâèõ øèðîêîëèñòÿ- íèõ ë³ñàõ Æèãóë³â (Ðîñ³ÿ, Ñàìàðñüêà îáë.). Îáãîâîðþþòüñÿ ïîä³áíîñò³ ³ â³äì³ííîñò³ P. wasseri ç áëèçüêèìè âèäàìè ðîäó. Òàêîæ äàºòüñÿ á³áë³îãðàô³ÿ ç ðîäó Pachykytospora. Ê ë þ ÷ î â ³ ñ ë î â à: Basidiomycetes Polyporales, øèðîêîëèñòÿí³ ë³ñè, Pachykytospora wasseri, íîâèé âèä

È.Â. Çìèòðîâè÷1, Â.Ô. Ìàëûøåâà1, Â.À. Ñïèðèí2 1 Áîòàíè÷åñêèé èíñòèòóò èì. Â.Ë. Êîìàðîâà ÐÀÍ, ã. Ñàíêò-Ïåòåðáóðã 2 Ñàíêò-Ïåòåðáóðãñêèé Ãóìàíèòàðíûé óíèâåðñèòåò ïðîôñîþçîâ

ÍÎÂÛÉ ÂÈÄ ÐÎÄÀ PACHYKYTOSPORA (BASIDIOMYCOTA, POLYPORALES) ÈÇ ÆÈÃÓËÅÉ (ÅÂÐÎÏÅÉÑÊÀß ÐÎÑÑÈß)

Îïèñàí íîâûé âèä ðîäà Pachykytospora — P. wasseri, îáíàðóæåííûé â òåíåâûõ øèðîêîëè- ñòâåííûõ ëåñàõ Æèãóëåé (Ðîññèÿ, Ñàìàðñêàÿ îáë.). Îáñóæäàþòñÿ ñõîäñòâà è ðàçëè÷èÿ P. wasseri ñ áëèçêèìè âèäàìè ðîäà. Äàåòñÿ òàêæå áèáëèîãðàôèÿ ïî ðîäó Pachykytospora. Ê ë þ ÷ å â û å ñ ë î â à: Basidiomycetes Polyporales, øèðîêîëèñòâåííûå ëåñà, Pachykyto- spora wasseri, íîâûé âèä

46 ISSN 0372-4123. Ukr. Botan. Journ., 2007, vol. 64, ¹ 1