ADH4 Antibody Cat

ADH4 Antibody Cat. No.: 55-047 ADH4 Antibody Western blot analysis of ADH4 using rabbit polyclonal ADH4 antibody immunohistochemistry analysis in ADH4 Antibody using 293 cell lysates (2 ug/lane) either formalin fixed and paraffin embedded human colon nontransfected (Lane 1) or transiently transfected (Lane 2) carcinoma followed by peroxidase conjugation of the with the ADH4 gene. secondary antibody and DAB staining. Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC- conjugated goat-anti-rabbit secondary antibodies were used for the analysis. Specifications September 25, 2021 1 https://www.prosci-inc.com/adh4-antibody-55-047.html HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse This ADH4 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 319-348 amino acids from the C-terminal region of human ADH4. TESTED APPLICATIONS: Flow, IHC-P, WB For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 PREDICTED MOLECULAR 40 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: ADH4 ALTERNATE NAMES: Alcohol dehydrogenase 4, Alcohol dehydrogenase class II pi chain, ADH4 ACCESSION NO.: P08319 PROTEIN GI NO.: 308153684 GENE ID: 127 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References September 25, 2021 2 https://www.prosci-inc.com/adh4-antibody-55-047.html This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and BACKGROUND: lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. REFERENCES: 1) Pochareddy, S., et al. Gene 460 (1-2), 1-7 (2010) : 2) Preuss, U.W., et al. Addict Biol (2010) In press : 3) Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010) 4) Rainero, I., et al. Headache 50(1):92-98(2010) ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 25, 2021 3 https://www.prosci-inc.com/adh4-antibody-55-047.html.

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Mouse Adh4 Knockout Project (CRISPR/Cas9)
https://www.alphaknockout.com Mouse Adh4 Knockout Project (CRISPR/Cas9) Objective: To create a Adh4 knockout Mouse model (C57BL/6J) by CRISPR/Cas-mediated genome engineering. Strategy summary: The Adh4 gene (NCBI Reference Sequence: NM_011996 ; Ensembl: ENSMUSG00000037797 ) is located on Mouse chromosome 3. 9 exons are identified, with the ATG start codon in exon 1 and the TGA stop codon in exon 9 (Transcript: ENSMUST00000013458). Exon 2~7 will be selected as target site. Cas9 and gRNA will be co-injected into fertilized eggs for KO Mouse production. The pups will be genotyped by PCR followed by sequencing analysis. Note: Exon 2 starts from about 1.68% of the coding region. Exon 2~7 covers 84.17% of the coding region. The size of effective KO region: ~9976 bp. The KO region does not have any other known gene. Page 1 of 8 https://www.alphaknockout.com Overview of the Targeting Strategy Wildtype allele 5' gRNA region gRNA region 3' 1 2 3 4 5 6 7 9 Legends Exon of mouse Adh4 Knockout region Page 2 of 8 https://www.alphaknockout.com Overview of the Dot Plot (up) Window size: 15 bp Forward Reverse Complement Sequence 12 Note: The 2000 bp section upstream of Exon 2 is aligned with itself to determine if there are tandem repeats. No significant tandem repeat is found in the dot plot matrix. So this region is suitable for PCR screening or sequencing analysis. Overview of the Dot Plot (down) Window size: 15 bp Forward Reverse Complement Sequence 12 Note: The 936 bp section downstream of Exon 7 is aligned with itself to determine if there are tandem repeats.
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