Product Datasheet

SEPT2 Antibody

Catalog No: #33053

Orders: [email protected] Description Support: [email protected]

Product Name SEPT2 Antibody

Host Species Rabbit

Clonality Polyclonal

Purification Antibodies were purified by affinity purification using immunogen.

Applications WB,IF

Species Reactivity Human,Mouse

Specificity The antibody detects endogenous level of total SEPT2 .

Immunogen Type Recombinant Protein

Immunogen Description Recombinant protein of human SEPT2.

Target Name SEPT2

Other Names DIFF6; NEDD5; NEDD-5; Pnutl3; hNedd5

Accession No. Swiss-Prot:Q15019NCBI ID:4735

SDS-PAGE MW 41KD

Concentration 1.0mg/ml

Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02%

sodium azide and 50% glycerol.

Storage Store at -20°C

Application Details

WB 1:500 - 1:2000IF 1:50 - 1:100

Images

Immunofluorescence analysis of U2OS cells using SEPT2 . Blue: DAPI for nuclear staining.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Western blot analysis of extracts of various cell lines, using SEPT2 at 1:1000 dilution.

Background

Filament-forming cytoskeletal GTPase. Required for normal organization of the cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Note: This product is for in vitro research use only and is not intended for use in humans or animals.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2