ZOONOSES MONITORING

Bulgaria

TRENDS AND SOURCES OF ZOONOSES AND ZOONOTIC AGENTS IN FOODSTUFFS, ANIMALS AND FEEDINGSTUFFS

including information on foodborne outbreaks, antimicrobial resistance in zoonotic and indicator bacteria and some pathogenic microbiological agents

IN 2019

Bulgaria - 2019 1 Bulgaria - 2019 Report on trends and sources of zoonoses

PREFACE

This report is submitted to the European Commission in accordance with Article 9 of Council Directive 2003/99/ EC*. The information has also been forwarded to the European Food Safety Authority (EFSA).

The report contains information on trends and sources of zoonoses and zoonotic agents in Bulgaria during the year 2019.

The information covers the occurrence of these diseases and agents in animals, foodstuffs and in some cases also in feedingstuffs. In addition the report includes data on antimicrobial resistance in some zoonotic agents and indicator bacteria as well as information on epidemiological investigations of foodborne outbreaks. Complementary data on susceptible animal populations in the country is also given. The information given covers both zoonoses that are important for the public health in the whole European Union as well as zoonoses, which are relevant on the basis of the national epidemiological situation. The report describes the monitoring systems in place and the prevention and control strategies applied in the country. For some zoonoses this monitoring is based on legal requirements laid down by the European Union legislation, while for the other zoonoses national approaches are applied.

The report presents the results of the examinations carried out in the reporting year. A national evaluation of the epidemiological situation, with special reference to trends and sources of zoonotic infections, is given. Whenever possible, the relevance of findings in foodstuffs and animals to zoonoses cases in humans is evaluated. The information covered by this report is used in the annual European Union Summary Reports on zoonoses and antimicrobial resistance that are published each year by EFSA.

The national report contains two parts: tables summarising data reported in the Data Collection Framework and the related text forms. The text forms were sent by email as pdf files and they are incorporated at the end of the report.

* Directive 2003/ 99/ EC of the European Parliament and of the Council of 12 December 2003 on the monitoring of zoonoses and zoonotic agents, amending Decision 90/ 424/ EEC and repealing Council Directive 92/ 117/ EEC, OJ L 325, 17.11.2003, p. 31

Bulgaria - 2019 2 List of Contents

ANIMAL POPULATION TABLES 3 DISEASE STATUS TABLES FOR BRUCELLA 4 Bovine brucellosis in countries and regions that do not receive Community co-financing for eradication programme 4 Ovine or Caprine brucellosis in countries and regions that do not receive Community co-financing for eradication programme 5 DISEASE STATUS TABLES FOR MYCOBACTERIUM 6 Bovine tuberculosis in countries and regions that do not receive Community co-financing for eradication programme 6 PREVALENCE TABLES 7 Brucella:BRUCELLA 7 animal 7 Campylobacter:CAMPYLOBACTER 8 animal 8 food 9 COXIELLA 10 animal 10 Cysticercus:CYSTICERCUS 11 animal 11 Echinococcus:ECHINOCOCCUS 12 animal 12 Enterococcus, non-pathogenic:ENTEROCOCCUS, NON-PATHOGENIC 13 food 13 Escherichia coli:ESCHERICHIA COLI 14 food 14 Escherichia coli, non-pathogenic:ESCHERICHIA COLI, NON-PATHOGENIC 15 food 15 FLAVIVIRUS 16 animal 16 HISTAMINE 17 food 17 Leptospira:LEPTOSPIRA 18 animal 18 Listeria:LISTERIA 19 animal 19 food 20 Lyssavirus:LYSSAVIRUS 40 animal 40 Marine biotoxins:MARINE BIOTOXINS 44 food 44 Salmonella:SALMONELLA 45 animal 45 food 46 feed 71 Staphylococcal enterotoxins:STAPHYLOCOCCAL ENTEROTOXINS 77 food 77 Staphylococcus:STAPHYLOCOCCUS AUREUS METICILLIN RESISTANT (MRSA) 78 food 78 Trichinella:TRICHINELLA 79 animal 79 food 81 Vibrio:VIBRIO 82 food 82 FOODBORNE OUTBREAKS TABLES 83 AMR TABLES FOR CAMPYLOBACTER 86 AMR TABLES FOR SALMONELLA 87 Salmonella Hiduddify 87 Meat from pig - fresh - Retail - Monitoring - active - Official sampling - OTHER AMR MON 87 N_A 87 Salmonella Infantis 88 Meat from pig - fresh - Slaughterhouse - Monitoring - Official sampling - OTHER AMR MON 88 N_A 88 Salmonella Rissen 89 Meat from pig - fresh - Retail - Monitoring - active - Official sampling - OTHER AMR MON 89 N_A 89 Salmonella Senftenberg 90 Meat from pig - carcase - Slaughterhouse - Monitoring - Official sampling - AMR MON 90 N_A 90 Salmonella Typhimurium 91 Meat from pig - carcase - Slaughterhouse - Monitoring - Official sampling - AMR MON 91 N_A 91 Salmonella Typhimurium, monophasic 92 Meat from pig - meat preparation - Retail - Monitoring - active - Official sampling - OTHER AMR MON 92 N_A 92 AMR TABLES FOR ESCHERICHIA COLI 93 Escherichia coli, non-pathogenic, unspecified 93 Meat from bovine animals - fresh - Retail - Monitoring - Official sampling - ESBL MON pnl2 93 N_A 93 Meat from bovine animals - fresh - Retail - Monitoring - Official sampling - ESBL MON 95 N_A 95 Pigs - fattening pigs - Slaughterhouse - Monitoring - Official sampling - AMR MON 97 N_A 97 Pigs - fattening pigs - Slaughterhouse - Monitoring - Official sampling - ESBL MON pnl2 99 N_A 99 Pigs - fattening pigs - Slaughterhouse - Monitoring - Official sampling - ESBL MON 101 N_A 101 Meat from pig - fresh - Retail - Monitoring - Official sampling - ESBL MON pnl2 103 N_A 103 Meat from pig - fresh - Retail - Monitoring - Official sampling - ESBL MON 105 N_A 105 OTHER AMR TABLES 107 ESBL 108 LATEST TRANSMISSIONS 110 ANIMAL POPULATION TABLES

Table Susceptible animal population

Metrics Population Animal species Category of animals Unit holding animal herd/flock Beehives Bee-colonies Bee-colonies 19,200 973,451 Cattle (bovine animals) Cattle (bovine animals) 47,245 Cattle (bovine animals) - adult cattle over 2 years 492,577 Cattle (bovine animals) - calves (under 1 year) 100,224 Cattle (bovine animals) - calves (under 1 year) - dairy calves 18,777 Cattle (bovine animals) - others 726 22,048 Cattle (bovine animals) - young cattle (1-2 years) 89,707 Ducks Ducks 1,408,000 Fish Fish 283 Fish - farmed 68 Fish - farmed - carp 499 Gallus gallus (fowl) Gallus gallus (fowl) 6,138,000 197 Gallus gallus (fowl) - broilers 6,921,000 201 Geese Geese 13,000 Goats Goats - animals over 1 year 14,405 Goats - animals under 1 year 19,775 Goats - meat production animals 27,418 Goats - milk goats 252,615 Pigs Pigs 1,169 23,969 Pigs - breeding animals - unspecified - sows 40,367 Pigs - fattening pigs - unspecified - piglets 542,366 Pigs - unspecified 33 2,126 Pigs - wild 89,000 Sheep Sheep - animals over 1 year 53,747 Sheep - animals under 1 year (lambs) 160,485 Sheep - meat production animals 144,679 Sheep - milk ewes 1,291,607 Sheep and goats Sheep and goats 53,116 Solipeds, domestic Solipeds, domestic 28,626 63,423 Turkeys Turkeys 35,000

Bulgaria - 2019 3 DISEASE STATUS TABLES

Table Bovine brucellosis in countries and regions that do not receive Community co-financing for eradication programme

Metrics Number of animals or Number of Number of Number of pools herds with herds animals tested status Number of tested tested Total under officially infected under under number of surveillance Region free herds surveillance surveillance herds by bulk milk BULGARIA 47,245 0 1,898 47,245 Габрово 3,231 Силистра 88 Варна 1,667 3,548 Добрич 131 Шумен 13,787 Търговище 10,309 Бургас 1,520 Сливен 20,242 София (столица) 7 1,132 София 2,989 4,652 Благоевград 26,882 Перник 381 2,508 Кюстендил 1,985 Пловдив 3,164 32,958 Хасково 3,171 Пазарджик 2,078 Смолян 1,657 Кърджали 5,556

Bulgaria - 2019 4 Table Ovine or Caprine brucellosis in countries and regions that do not receive Community co-financing for eradication programme

Metrics Number of Number of Number of herds with herds animals status Number of tested tested Total officially infected under under number of Region free herds surveillance surveillance herds BULGARIA 53,116 0 282 53,116 Силистра 862 Варна 6,526 Добрич 6,552 Бургас 3,973 София (столица) 1,257 София 8,704 Благоевград 57,965 Перник 2,285 Кюстендил 4,347 Пловдив 23,892 Хасково 6,894 Пазарджик 9,727 Смолян 10,492 Кърджали 9,586

Bulgaria - 2019 5 DISEASE STATUS TABLES

Table Bovine tuberculosis in countries and regions that do not receive Community co-financing for eradication programme

Metrics Number of animals with suspicious lesions of tuberculosis examined and submitted to Number of animals histopathological and detected positive in Number of herds with Number of infected bacteriological bacteriological Region status officially free herds examinations examination Total number of herds BULGARIA 47,238 7 47,245 Габрово 5 5 Стара Загора 3 2 Пловдив 9 7 Пазарджик 62 31 Кърджали 2 1

Bulgaria - 2019 6 PREVALENCE TABLES

Table Brucella:BRUCELLA in animal

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Not Available Dogs - Farm - Bulgaria - animal sample - blood - Surveillance - Official sampling - Objective sampling N_A Not Available animal 2 2 Brucella 2 Pigs - breeding animals - raised under controlled housing conditions - sows and boars - Farm - Bulgaria - N_A Complement animal 72 0 Brucella 0 animal sample - blood - Surveillance - Official sampling - Objective sampling fixation test (CFT) Pigs - Farm - Bulgaria - animal sample - blood - Surveillance - Official sampling - Objective sampling N_A Not Available animal 10 10 Brucella 10 Pigs - Farm - Bulgaria - animal sample - blood - Surveillance - Official sampling - Objective sampling N_A Complement animal 3242 0 Brucella 0 fixation test (CFT)

Bulgaria - 2019 7 Table Campylobacter:CAMPYLOBACTER in animal

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Not Available Gallus gallus (fowl) - broilers - Slaughterhouse - Bulgaria - animal sample - faeces - Surveillance - HACCP N_A ISO 10272- slaughte 30 26 Campylobacter jejuni 26 and own check - Objective sampling 2:2017 r animal Campylobacter batch Gallus gallus (fowl) - broilers - Slaughterhouse - Bulgaria - animal sample - faeces - Surveillance - HACCP N_A ISO 10272- herd/floc 58 0 Campylobacter 0 and own check - Objective sampling 1:2017 k Campylobacter

Bulgaria - 2019 8 Table Campylobacter:CAMPYLOBACTER in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from broilers (Gallus gallus) - carcase - chilled - Processing plant - single 10 Gram N_A ISO 10272- 160 15 Campylobacter jejuni 15 Bulgaria - food sample - neck skin - Surveillance - based on Regulation (food/fee 2:2017 2073 - Official sampling - Objective sampling d) Campylobacter Meat from broilers (Gallus gallus) - carcase - chilled - Slaughterhouse - batch 10 Gram N_A ISO 10272- 33 6 Campylobacter jejuni 6 Bulgaria - food sample - neck skin - Surveillance - based on Regulation (food/fee 2:2017 2073 - HACCP and own check - Objective sampling d) Campylobacter Meat from broilers (Gallus gallus) - carcase - chilled - Slaughterhouse - single 10 Gram N_A ISO 10272- 285 71 Campylobacter jejuni 71 Bulgaria - food sample - neck skin - Surveillance - based on Regulation (food/fee 2:2017 2073 - Official sampling - Objective sampling d) Campylobacter Meat from duck - carcase - chilled - Slaughterhouse - Bulgaria - food batch 10 Gram N_A ISO 10272- 15 0 Campylobacter 0 sample - neck skin - Surveillance - based on Regulation 2073 - Official (food/fee 2:2017 sampling - Objective sampling d) Campylobacter

Bulgaria - 2019 9 Table COXIELLA in animal

Total Total N of clinical Sampling units units affected Metrics N of units Area of Sampling Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling strategy unit Sampling Details Method tested positive herds Zoonoses positive Not Available Cattle (bovine animals) - Farm - Bulgaria - animal sample - blood - Clinical investigations - Official sampling - Suspect animal N_A Enzyme-linked 1 0 Coxiella burnetii sampling immunosorbent 0 assay (ELISA) Cattle (bovine animals) - Farm - Bulgaria - animal sample - blood - Surveillance - Official sampling - Objective sampling animal N_A Enzyme-linked 298 28 Coxiella burnetii immunosorbent 28 assay (ELISA) Cattle (bovine animals) - Farm - Bulgaria - animal sample - placental swab - Clinical investigations - Official sampling - animal N_A PCR 2 1 Coxiella burnetii 1 Suspect sampling Goats - Farm - Bulgaria - animal sample - blood - Clinical investigations - Official sampling - Suspect sampling animal N_A Enzyme-linked 5 0 Coxiella burnetii immunosorbent 0 assay (ELISA) Goats - Farm - Bulgaria - animal sample - blood - Surveillance - Official sampling - Objective sampling animal N_A Enzyme-linked 28 2 Coxiella burnetii immunosorbent 2 assay (ELISA) Goats - Farm - Bulgaria - animal sample - placental swab - Clinical investigations - Official sampling - Suspect sampling animal N_A PCR 3 0 Coxiella burnetii 0 Sheep - Farm - Bulgaria - animal sample - blood - Clinical investigations - Official sampling - Suspect sampling animal N_A Enzyme-linked 13 6 Coxiella burnetii immunosorbent 6 assay (ELISA) Sheep - Farm - Bulgaria - animal sample - blood - Surveillance - Official sampling - Objective sampling animal N_A Enzyme-linked 211 19 Coxiella burnetii immunosorbent 19 assay (ELISA) Sheep - Farm - Bulgaria - animal sample - placental swab - Clinical investigations - Official sampling - Suspect sampling animal N_A PCR 8 2 Coxiella burnetii 2

Bulgaria - 2019 10 Table Cysticercus:CYSTICERCUS in animal

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Not Available Cattle (bovine animals) - Slaughterhouse - Not Available - Not Available - Monitoring - active - Official N_A Not Available animal 29274 16 Cysticercus 16 sampling - Census Pigs - breeding animals - raised under controlled housing conditions - Slaughterhouse - Not Available - Not N_A Not Available animal 11960 5 Cysticercus 5 Available - Monitoring - active - Official sampling - Census 86 Sheep and goats - Slaughterhouse - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 23528 1094 Cysticercus 1,094 Census 6

Bulgaria - 2019 11 Table Echinococcus:ECHINOCOCCUS in animal

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Not Available Cattle (bovine animals) - Slaughterhouse - Not Available - Not Available - Monitoring - active - Official N_A Not Available animal 29274 947 Echinococcus 947 sampling - Census Pigs - breeding animals - raised under controlled housing conditions - Slaughterhouse - Not Available - Not N_A Not Available animal 11960 337 Echinococcus 337 Available - Monitoring - active - Official sampling - Census 86 Sheep and goats - Slaughterhouse - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 23528 1833 Echinococcus 1,833 Census 6

Bulgaria - 2019 12 Table Enterococcus, non-pathogenic:ENTEROCOCCUS, NON-PATHOGENIC in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Water - potable water - Processing plant - Bulgaria - food sample - single 100 Millilitre N_A Not Available 337 0 Enterococcus, non-pathogenic 0 Surveillance - HACCP and own check - Objective sampling (food/fee d)

Bulgaria - 2019 13 Table Escherichia coli:ESCHERICHIA COLI in food

M et Sample ri Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler Sample weight total units total units c Area of sampling - Sampling strategy Sampling unit weight unit Sampling Details Method tested positive Zoonoses ANTH VTX AG s N units positive Not Available Seeds, sprouted - ready-to-eat - Processing plant - Bulgaria - food sample - Surveillance - batch 25 Gram N_A ISO/TS 120 0 Verocytotoxi H-antigen Verotoxin eae based on Regulation 2073 - HACCP and own check - Objective sampling (food/feed) 13136:2012 genic E. coli unknown production, negative (including the (VTEC) toxin type EU-RL unknown 0 adaptation for O104:H4) Seeds, sprouted - ready-to-eat - Processing plant - Bulgaria - food sample - Surveillance - single 25 Gram N_A ISO/TS 10 0 Verocytotoxi H-antigen Not eae based on Regulation 2073 - Official sampling - Objective sampling (food/feed) 13136:2012 genic E. coli unknown Available negative (including the (VTEC) EU-RL 0 adaptation for O104:H4) Seeds, sprouted - ready-to-eat - Restaurant or Cafe or Pub or Bar or Hotel or Catering single 25 Gram N_A ISO/TS 5 0 Verocytotoxi H-antigen Verotoxin eae service - Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/feed) 13136:2012 genic E. coli unknown production, negative sampling - Objective sampling (including the (VTEC) toxin type EU-RL unknown 0 adaptation for O104:H4) Seeds, sprouted - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - based on single 25 Gram N_A ISO/TS 35 0 Verocytotoxi H-antigen Verotoxin eae Regulation 2073 - Official sampling - Objective sampling (food/feed) 13136:2012 genic E. coli unknown production, negative (including the (VTEC) toxin type EU-RL unknown 0 adaptation for O104:H4)

Bulgaria - 2019 14 Table Escherichia coli, non-pathogenic:ESCHERICHIA COLI, NON-PATHOGENIC in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Bakery products - bread - Processing plant - Bulgaria - food sample - single 1 Gram N_A Not Available 18 0 Escherichia coli, non- 0 Surveillance - HACCP and own check - Objective sampling (food/fee pathogenic d) Bakery products - cakes - Processing plant - Bulgaria - food sample - single 1 Gram N_A Not Available 12 0 Escherichia coli, non- 0 Surveillance - HACCP and own check - Objective sampling (food/fee pathogenic d) Cheeses made from cows' milk - hard - Processing plant - Bulgaria - food single 1 Gram N_A Not Available 17 0 Escherichia coli, non- 0 sample - milk - Surveillance - HACCP and own check - Objective sampling (food/fee pathogenic d) Cheeses made from cows' milk - Processing plant - Bulgaria - food sample single 1 Gram N_A Not Available 7 0 Escherichia coli, non- 0 - milk - Surveillance - HACCP and own check - Objective sampling (food/fee pathogenic d) Cheeses made from cows' milk - soft and semi-soft - made from single 1 Gram N_A Not Available 12 0 Escherichia coli, non- 0 pasteurised milk - Processing plant - Bulgaria - food sample - milk - (food/fee pathogenic Surveillance - HACCP and own check - Objective sampling d) Cheeses made from goats' milk - Processing plant - Bulgaria - food sample single 1 Gram N_A Not Available 5 0 Escherichia coli, non- 0 - milk - Surveillance - HACCP and own check - Objective sampling (food/fee pathogenic d) Cheeses, made from mixed milk from cows, sheep and/or goats - single 1 Gram N_A Not Available 3 0 Escherichia coli, non- 0 Processing plant - Bulgaria - food sample - milk - Surveillance - HACCP (food/fee pathogenic and own check - Objective sampling d) Dairy products (excluding cheeses) - butter - Processing plant - Bulgaria - single 1 Gram N_A Not Available 13 0 Escherichia coli, non- 0 food sample - milk - Surveillance - HACCP and own check - Objective (food/fee pathogenic sampling d) Meat from broilers (Gallus gallus) - fresh - chilled - Processing plant - single 1 Gram N_A Not Available 97 0 Escherichia coli, non- 0 Bulgaria - food sample - meat - Surveillance - HACCP and own check - (food/fee pathogenic Objective sampling d) Meat from pig - meat products - raw but intended to be eaten cooked - single 1 Gram N_A Not Available 150 0 Escherichia coli, non- 0 chilled - Processing plant - Bulgaria - food sample - meat - Surveillance - (food/fee pathogenic HACCP and own check - Objective sampling d) Molluscan shellfish - cooked - frozen - Processing plant - Bulgaria - food single 1 Gram N_A Not Available 43 0 Escherichia coli, non- 0 sample - Surveillance - HACCP and own check - Objective sampling (food/fee pathogenic d) Molluscan shellfish - raw - Processing plant - Bulgaria - food sample - single 1 Gram N_A Not Available 34 0 Escherichia coli, non- 0 Surveillance - HACCP and own check - Objective sampling (food/fee pathogenic d) Sauce and dressings - mayonnaise - Processing plant - Bulgaria - food single 1 Gram N_A Not Available 45 0 Escherichia coli, non- 0 sample - Surveillance - HACCP and own check - Objective sampling (food/fee pathogenic d) Water - potable water - Processing plant - Bulgaria - food sample - single 100 Millilitre N_A Not Available 420 0 Escherichia coli, non- 0 Surveillance - HACCP and own check - Objective sampling (food/fee pathogenic d)

Bulgaria - 2019 15 Table FLAVIVIRUS in animal

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling Vaccination units units Metrics N of units Area of Sampling strategy unit status Sampling Details Method tested positive Zoonoses positive Not Available All animals - farmed - Farm - Not Available - Not Available - Surveillance - Not applicable - Other animal No N_A IgG ELISA 2 2 West Nile virus 2 All animals - farmed - Farm - Not Available - Not Available - Surveillance - Not applicable - Other animal No N_A PCR 2 0 West Nile virus 0 Birds - wild - Natural habitat - Not Available - Not Available - Surveillance - Not applicable - Other animal No N_A PCR 37 0 West Nile virus 0 Dogs - pet animals - Veterinary clinics - Not Available - Not Available - Surveillance - Not applicable - Other animal No N_A PCR 1 0 West Nile virus 0

Bulgaria - 2019 16 Table HISTAMINE in food

Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Fish - Fishery products from fish species associated with a high amount of histidine - not single 5 Gram N_A 1 0 <= 100 Histamine 0 0 enzyme maturated - Border Control Posts - Not Available - Not Available - Monitoring - Official (food/fee >100 TO <= Histamine 0 0 sampling - Objective sampling d) 200 >200 Histamine 0 0 Fish - Fishery products from fish species associated with a high amount of histidine - not single 5 Gram N_A 10 0 <= 100 Histamine 0 0 enzyme maturated - Retail - Not Available - Not Available - Monitoring - Official sampling - (food/fee >100 TO <= Histamine 0 0 Objective sampling d) 200 >200 Histamine 0 0 Fish - Fishery products from fish species associated with a high amount of histidine - not single 5 Gram N_A 3 0 <= 100 Histamine 0 0 enzyme maturated - Wholesale - Not Available - Not Available - Monitoring - HACCP and own (food/fee >100 TO <= Histamine 0 0 check - Objective sampling d) 200 >200 Histamine 0 0 Fish - marinated - Retail - Not Available - Not Available - Monitoring - Official sampling - single 5 Gram N_A 1 0 > 400 Histamine 0 0 Objective sampling (food/fee >200 TO <= Histamine 0 0 d) 400 <=200 Histamine 0 0 Fish - raw - frozen - Border Control Posts - Not Available - Not Available - Monitoring - Official single 5 Gram N_A 4 0 <= 100 Histamine 0 0 sampling - Objective sampling (food/fee >100 TO <= Histamine 0 0 d) 200 >200 Histamine 0 0 Fish - raw - frozen - Retail - Not Available - Not Available - Monitoring - Official sampling - single 5 Gram N_A 40 0 <= 100 Histamine 0 0 Objective sampling (food/fee >100 TO <= Histamine 0 0 d) 200 >200 Histamine 0 0 Fish - raw - frozen - Wholesale - Not Available - Not Available - Monitoring - Official sampling - single 5 Gram N_A 4 0 <= 100 Histamine 0 0 Objective sampling (food/fee >100 TO <= Histamine 0 0 d) 200 >200 Histamine 0 0 Fish - smoked - Retail - Not Available - Not Available - Monitoring - Official sampling - single 5 Gram N_A 4 0 > 400 Histamine 0 0 Objective sampling (food/fee >200 TO <= Histamine 0 0 d) 400 <=200 Histamine 0 0 Fish - smoked - Wholesale - Not Available - Not Available - Monitoring - Official sampling - single 5 Gram N_A 1 0 > 400 Histamine 0 0 Objective sampling (food/fee >200 TO <= Histamine 0 0 d) 400 <=200 Histamine 0 0

Bulgaria - 2019 17 Table Leptospira:LEPTOSPIRA in animal

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Not Available Cattle (bovine animals) - adult cattle over 2 years - Farm - Not Available - animal sample - blood - N_A Modified animal 1857 0 Leptospira 0 Surveillance - Official sampling - Objective sampling agglutination test (MAT) Cattle (bovine animals) - adult cattle over 2 years - Farm - Not Available - Not Available - Surveillance - N_A Not Available animal 1799 0 Leptospira 0 Official sampling - Objective sampling Cattle (bovine animals) - Farm - Bulgaria - animal sample - blood - Surveillance - Official sampling - Objective N_A Modified animal 2632 0 Leptospira 0 sampling agglutination test (MAT) Pigs - Farm - Bulgaria - animal sample - blood - Surveillance - Official sampling - Objective sampling N_A Modified animal 27 0 Leptospira 0 agglutination test (MAT) Pigs - Farm - Not Available - Not Available - Surveillance - Official sampling - Objective sampling N_A Not Available animal 249 0 Leptospira 0

Bulgaria - 2019 18 Table Listeria:LISTERIA in animal

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Not Available Sheep - Farm - Bulgaria - animal sample - brain - Clinical investigations - Official sampling - Objective N_A Detection animal 1 1 Listeria monocytogenes O4 1 sampling method presence in x g Shellfish - farmed - Processing plant - Bulgaria - animal sample - Surveillance - based on Regulation 2073 - N_A Detection animal 1 1 Listeria monocytogenes O1/2 1 Official sampling - Objective sampling method presence in x g

Bulgaria - 2019 19 Table Listeria:LISTERIA in food

Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Bakery products - cakes - Processing plant - Bulgaria - food sample - Surveillance - HACCP single 25 Gram N_A 26 0 detection Listeria monocytogenes and own check - Objective sampling (food/fee 26 0 d) Bakery products - desserts - containing heat-treated cream - Processing plant - Not Available single 25 Gram N_A 4 0 detection Listeria monocytogenes - Not Available - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 4 0 sampling d) Bakery products - desserts - containing heat-treated cream - Retail - Bulgaria - food sample - batch 25 Gram N_A 12 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 12 0 d) Bakery products - desserts - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - single 1 Gram Only enumeration 3 0 <= 100 Listeria monocytogenes 3 0 Bulgaria - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 3 0 Bakery products - desserts - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - single 25 Gram Only detection 8 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - Official sampling - Objective sampling (food/fee 8 0 d) Bakery products - desserts - Retail - Bulgaria - food sample - Surveillance - Official sampling - batch 25 Gram N_A 1 0 detection Listeria monocytogenes Objective sampling (food/fee 1 0 d) Bakery products - pastry - Game handling establishment - Not Available - Not Available - batch 200 Gram N_A 38 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 38 0 d) Bakery products - pastry - Processing plant - Bulgaria - food sample - Surveillance - HACCP single 25 Gram N_A 1 1 detection Listeria monocytogenes O1/2 and own check - Objective sampling (food/fee 1 1 d) Bakery products - pastry - Retail - Bulgaria - food sample - Surveillance - Official sampling - single 1 Gram N_A 5 0 detection Listeria monocytogenes Objective sampling (food/fee 5 0 d) Beverages, non-alcoholic - soft drinks - Processing plant - Bulgaria - food sample - batch 25 Gram N_A 2 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 2 0 d) Cereals and meals - flakes - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - single 1 Gram N_A 10 0 <= 100 Listeria monocytogenes 10 0 Not Available - Not Available - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 10 0 Cheeses made from cows' milk - curd - Catering - Bulgaria - food sample - Surveillance - single 25 Gram N_A 5 0 detection Listeria monocytogenes based on Regulation 2073 - Official sampling - Objective sampling (food/fee 5 0 d) Cheeses made from cows' milk - curd - Processing plant - Bulgaria - food sample - milk - single 25 Gram N_A 5 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 5 0 d) Cheeses made from cows' milk - curd - Processing plant - Bulgaria - food sample - single 25 Gram N_A 1 0 detection Listeria monocytogenes 1 0 Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) 5 0 detection Listeria monocytogenes 5 0 Cheeses made from cows' milk - curd - Processing plant - Bulgaria - food sample - single 25 Gram N_A 1 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 1 0 d) Cheeses made from cows' milk - curd - Processing plant - Not Available - food sample - single 1 Gram only enumeration 5 0 <= 100 Listeria monocytogenes 5 0 Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Cheeses made from cows' milk - curd - Processing plant - Not Available - food sample - single 25 Gram only detection 25 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 25 0 d) Cheeses made from cows' milk - curd - Processing plant - Not Available - Not Available - single 25 Gram N_A 12 0 detection Listeria monocytogenes 12 0 Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) 15 0 detection Listeria monocytogenes 15 0 Cheeses made from cows' milk - curd - Retail - Bulgaria - food sample - Surveillance - based single 25 Gram N_A 1 0 detection Listeria monocytogenes on Regulation 2073 - Official sampling - Objective sampling (food/fee 1 0 d) Cheeses made from cows' milk - curd - Retail - Not Available - food sample - Surveillance - single 1 Gram N_A 20 0 <= 100 Listeria monocytogenes 20 0 Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 20 0 Cheeses made from cows' milk - curd - Retail - Not Available - Not Available - Surveillance - single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0

Bulgaria - 2019 20 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Cheeses made from cows' milk - fresh - made from pasteurised milk - Processing plant - single 25 Gram N_A 150 5 detection Listeria monocytogenes 150 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Objective sampling d) Listeria monocytogenes O1/2 150 5 Cheeses made from cows' milk - fresh - made from pasteurised milk - Processing plant - Not single 25 Gram N_A 35 0 detection Listeria monocytogenes Available - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 35 0 Objective sampling d) Cheeses made from cows' milk - fresh - made from pasteurised milk - Retail - Bulgaria - food single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 sample - Surveillance - based on Regulation 2073 - HACCP and own check - Objective (food/fee sampling d) >100 Listeria monocytogenes 5 0 Cheeses made from cows' milk - fresh - made from pasteurised milk - Retail - Bulgaria - food single 1 Gram N_A 65 0 <= 100 Listeria monocytogenes 65 0 sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 65 0 Cheeses made from cows' milk - fresh - Processing plant - Not Available - Not Available - single 25 Gram N_A 2 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 2 0 d) Cheeses made from cows' milk - hard - made from pasteurised milk - Border Control Posts - single 25 Gram N_A 1 0 detection Listeria monocytogenes Serbia - food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1 0 sampling d) Cheeses made from cows' milk - hard - made from pasteurised milk - Processing plant - batch 25 Gram N_A 20 0 detection Listeria monocytogenes Bulgaria - food sample - milk - Surveillance - HACCP and own check - Objective sampling (food/fee 20 0 d) single 25 Millilitre N_A 120 0 detection Listeria monocytogenes (food/fee 120 0 d) Cheeses made from cows' milk - hard - made from pasteurised milk - Processing plant - batch 25 Gram N_A 15 0 detection Listeria monocytogenes Bulgaria - food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 15 0 d) single 25 Millilitre N_A 55 0 detection Listeria monocytogenes (food/fee 55 0 d) Cheeses made from cows' milk - hard - made from pasteurised milk - Processing plant - single 25 Gram N_A 5 0 detection Listeria monocytogenes 5 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Objective sampling d) 25 0 detection Listeria monocytogenes 25 0 Cheeses made from cows' milk - hard - made from pasteurised milk - Processing plant - Not single 1 Gram only enumeration 5 0 <= 100 Listeria monocytogenes 5 0 Available - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Objective sampling d) >100 Listeria monocytogenes 5 0 Cheeses made from cows' milk - hard - made from pasteurised milk - Processing plant - Not single 25 Gram only detection 210 0 detection Listeria monocytogenes Available - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 210 0 Objective sampling d) Cheeses made from cows' milk - hard - made from pasteurised milk - Retail - Bulgaria - food single 25 Gram N_A 25 0 <= 100 Listeria monocytogenes 25 0 sample - milk - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 25 0 Cheeses made from cows' milk - hard - made from pasteurised milk - Retail - Bulgaria - food single 1 Gram N_A 60 0 <= 100 Listeria monocytogenes 60 0 sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 60 0 Cheeses made from cows' milk - hard - made from pasteurised milk - Retail - Not Available - single 1 Gram only enumeration 145 0 <= 100 Listeria monocytogenes 145 0 food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 145 0 Cheeses made from cows' milk - hard - made from pasteurised milk - Retail - Not Available - single 25 Gram only detection 25 0 detection Listeria monocytogenes food sample - Surveillance - Official sampling - Objective sampling (food/fee 25 0 d) Cheeses made from cows' milk - hard - made from raw or low heat-treated milk - Processing single 25 Gram N_A 17 0 detection Listeria monocytogenes plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 17 0 Objective sampling d) Cheeses made from cows' milk - hard - made from raw or low heat-treated milk - Processing single 25 Gram N_A 5 0 detection Listeria monocytogenes plant - Bulgaria - food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 5 0 d) Cheeses made from cows' milk - hard - made from raw or low heat-treated milk - Retail - single 25 Gram N_A 10 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 10 0 Objective sampling d) Cheeses made from cows' milk - hard - made from raw or low heat-treated milk - Retail - Not single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 Available - Not Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Objective sampling d) >100 Listeria monocytogenes 5 0 Cheeses made from cows' milk - hard - Processing plant - Bulgaria - food sample - milk - single 25 Gram N_A 147 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 147 0 d)

Bulgaria - 2019 21 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Cheeses made from cows' milk - hard - Processing plant - Bulgaria - food sample - milk - single 25 Gram N_A 100 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 100 0 d) Cheeses made from cows' milk - hard - Processing plant - Not Available - Not Available - single 25 Gram N_A 26 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 26 0 d) Cheeses made from cows' milk - hard - Processing plant - Not Available - Not Available - single 25 Gram N_A 8 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 8 0 d) Cheeses made from cows' milk - hard - Retail - Bulgaria - food sample - milk - Surveillance - single 1 Gram Only enumeration 55 0 <= 100 Listeria monocytogenes 55 0 Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 55 0 Cheeses made from cows' milk - hard - Retail - Bulgaria - food sample - milk - Surveillance - single 25 Gram Only detection 15 0 detection Listeria monocytogenes Official sampling - Objective sampling (food/fee 15 0 d) Cheeses made from cows' milk - soft and semi-soft - made from pasteurised milk - Border single 25 Gram N_A 20 0 detection Listeria monocytogenes Control Posts - Serbia - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 20 0 sampling - Objective sampling d) Cheeses made from cows' milk - soft and semi-soft - made from pasteurised milk - Processing single 1 Gram N_A 12 0 <= 100 Listeria monocytogenes 12 0 plant - Bulgaria - food sample - milk - Surveillance - HACCP and own check - Objective (food/fee sampling d) >100 Listeria monocytogenes 12 0 Cheeses made from cows' milk - soft and semi-soft - made from pasteurised milk - Processing batch 25 Gram N_A 30 0 detection Listeria monocytogenes plant - Bulgaria - food sample - milk - Surveillance - HACCP and own check - Objective (food/fee 30 0 sampling d) single 25 Millilitre N_A 35 0 detection Listeria monocytogenes (food/fee 35 0 d) Cheeses made from cows' milk - soft and semi-soft - made from pasteurised milk - Processing single 1 Gram Only enumeration 10 0 <= 100 Listeria monocytogenes 10 0 plant - Bulgaria - food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 10 0 Cheeses made from cows' milk - soft and semi-soft - made from pasteurised milk - Processing batch 25 Gram N_A 125 0 detection Listeria monocytogenes plant - Bulgaria - food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 125 0 d) single 25 Gram Only detection 70 0 detection Listeria monocytogenes 70 0 (food/fee N_A d) Millilitre 90 0 detection Listeria monocytogenes 90 0 Cheeses made from cows' milk - soft and semi-soft - made from pasteurised milk - Processing single 25 Gram N_A 10 0 detection Listeria monocytogenes 10 0 plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Objective sampling d) 17 0 detection Listeria monocytogenes 17 0 Cheeses made from cows' milk - soft and semi-soft - made from pasteurised milk - Processing single 25 Gram N_A 10 0 detection Listeria monocytogenes plant - Bulgaria - food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 10 0 d) Cheeses made from cows' milk - soft and semi-soft - made from pasteurised milk - Retail - single 1 Gram Only enumeration 20 0 <= 100 Listeria monocytogenes 20 0 Bulgaria - food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 20 0 Cheeses made from cows' milk - soft and semi-soft - made from pasteurised milk - Retail - single 25 Gram Only detection 10 0 detection Listeria monocytogenes 10 0 Bulgaria - food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee N_A d) Millilitre 35 0 detection Listeria monocytogenes 35 0 Cheeses made from cows' milk - soft and semi-soft - made from pasteurised milk - Retail - single 25 Gram N_A 36 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 36 0 Objective sampling d) Cheeses made from cows' milk - soft and semi-soft - Processing plant - Bulgaria - food single 25 Gram N_A 25 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 25 0 d) Cheeses made from cows' milk - soft and semi-soft - Processing plant - Not Available - Not single 25 Gram N_A 4 0 detection Listeria monocytogenes Available - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 4 0 d) Cheeses made from cows' milk - soft and semi-soft - Processing plant - Not Available - Not single 25 Gram N_A 3 0 detection Listeria monocytogenes Available - Surveillance - HACCP and own check - Objective sampling (food/fee 3 0 d) Cheeses made from cows' milk - soft and semi-soft - Retail - Bulgaria - food sample - single 1 Gram N_A 20 0 <= 100 Listeria monocytogenes 20 0 Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 20 0 Cheeses made from cows' milk - soft and semi-soft - Retail - Not Available - food sample - single 1 Gram only enumeration 65 0 <= 100 Listeria monocytogenes 65 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 65 0

Bulgaria - 2019 22 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Cheeses made from cows' milk - soft and semi-soft - Retail - Not Available - food sample - single 25 Gram only detection 5 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 5 0 d) Cheeses made from cows' milk - unspecified - made from pasteurised milk - Game handling batch 200 Gram N_A 261 0 detection Listeria monocytogenes establishment - Not Available - Not Available - Surveillance - HACCP and own check - (food/fee 261 0 Objective sampling d) Cheeses made from goats' milk - fresh - made from pasteurised milk - Processing plant - batch 25 Gram N_A 30 0 detection Listeria monocytogenes Bulgaria - food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 30 0 d) Cheeses made from goats' milk - hard - made from pasteurised milk - Processing plant - Not single 25 Gram N_A 10 0 detection Listeria monocytogenes Available - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 10 0 Objective sampling d) Cheeses made from goats' milk - hard - Processing plant - Bulgaria - food sample - milk - single 25 Gram N_A 50 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 50 0 d) Cheeses made from goats' milk - hard - Processing plant - Bulgaria - food sample - milk - single 25 Gram N_A 5 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 5 0 d) Cheeses made from goats' milk - hard - Processing plant - Not Available - Not Available - single 25 Gram N_A 15 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 15 0 d) Cheeses made from goats' milk - hard - Processing plant - Not Available - Not Available - single 25 Gram N_A 2 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 2 0 d) Cheeses made from goats' milk - soft and semi-soft - made from pasteurised milk - single 25 Gram N_A 15 0 detection Listeria monocytogenes Processing plant - Bulgaria - food sample - milk - Surveillance - HACCP and own check - (food/fee 15 0 Objective sampling d) Cheeses made from goats' milk - soft and semi-soft - made from pasteurised milk - single 25 Gram N_A 1 0 detection Listeria monocytogenes 1 0 Processing plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/fee sampling - Objective sampling d) 5 0 detection Listeria monocytogenes 5 0 Cheeses made from goats' milk - soft and semi-soft - Processing plant - Not Available - Not single 25 Gram N_A 16 0 detection Listeria monocytogenes Available - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 16 0 d) Cheeses made from goats' milk - soft and semi-soft - Processing plant - Not Available - Not single 25 Gram N_A 2 0 detection Listeria monocytogenes Available - Surveillance - HACCP and own check - Objective sampling (food/fee 2 0 d) Cheeses made from sheep's milk - hard - made from pasteurised milk - Processing plant - Not single 25 Gram N_A 5 0 detection Listeria monocytogenes Available - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 5 0 Objective sampling d) Cheeses made from sheep's milk - hard - made from pasteurised milk - Retail - Not Available single 1 Gram only enumeration 5 0 <= 100 Listeria monocytogenes 5 0 - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Cheeses made from sheep's milk - hard - made from pasteurised milk - Retail - Not Available single 25 Gram only detection 10 0 detection Listeria monocytogenes - food sample - Surveillance - Official sampling - Objective sampling (food/fee 10 0 d) Cheeses made from sheep's milk - hard - Processing plant - Bulgaria - food sample - milk - single 25 Gram N_A 120 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 120 0 d) Cheeses made from sheep's milk - hard - Processing plant - Bulgaria - food sample - milk - single 25 Gram N_A 5 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 5 0 d) Cheeses made from sheep's milk - hard - Processing plant - Not Available - Not Available - single 25 Gram N_A 21 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 21 0 d) Cheeses made from sheep's milk - hard - Processing plant - Not Available - Not Available - single 25 Gram N_A 3 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 3 0 d) Cheeses made from sheep's milk - soft and semi-soft - made from pasteurised milk - single 25 Gram N_A 10 0 detection Listeria monocytogenes 10 0 Processing plant - Bulgaria - food sample - milk - Surveillance - HACCP and own check - (food/fee N_A Objective sampling d) Millilitre 15 0 detection Listeria monocytogenes 15 0 Cheeses made from sheep's milk - soft and semi-soft - made from pasteurised milk - single 25 Gram N_A 3 0 detection Listeria monocytogenes Processing plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 3 0 sampling - Objective sampling d) Cheeses made from sheep's milk - soft and semi-soft - made from pasteurised milk - Retail - single 25 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 Bulgaria - food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0

Bulgaria - 2019 23 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Cheeses made from sheep's milk - soft and semi-soft - made from pasteurised milk - Retail - single 25 Gram N_A 1 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1 0 Objective sampling d) Cheeses made from sheep's milk - soft and semi-soft - Processing plant - Not Available - Not single 25 Gram N_A 18 0 detection Listeria monocytogenes Available - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 18 0 d) Cheeses made from sheep's milk - soft and semi-soft - Processing plant - Not Available - Not single 25 Gram N_A 5 0 detection Listeria monocytogenes Available - Surveillance - HACCP and own check - Objective sampling (food/fee 5 0 d) Cheeses, made from mixed milk from cows, sheep and/or goats - fresh - Retail - Bulgaria - single 1 Gram Only enumeration 30 0 <= 100 Listeria monocytogenes 30 0 food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 30 0 Cheeses, made from mixed milk from cows, sheep and/or goats - fresh - Retail - Bulgaria - single 25 Gram Only detection 10 0 detection Listeria monocytogenes food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 10 0 d) Cheeses, made from mixed milk from cows, sheep and/or goats - hard - Processing plant - single 25 Gram N_A 38 0 detection Listeria monocytogenes Bulgaria - food sample - milk - Surveillance - HACCP and own check - Objective sampling (food/fee 38 0 d) Cheeses, made from mixed milk from cows, sheep and/or goats - hard - Processing plant - single 25 Gram N_A 10 0 detection Listeria monocytogenes Bulgaria - food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 10 0 d) Cheeses, made from mixed milk from cows, sheep and/or goats - hard - Processing plant - single 25 Gram N_A 14 0 detection Listeria monocytogenes Not Available - Not Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 14 0 Objective sampling d) Cheeses, made from mixed milk from cows, sheep and/or goats - soft and semi-soft - made batch 25 Gram N_A 10 0 detection Listeria monocytogenes from pasteurised milk - Processing plant - Bulgaria - food sample - milk - Surveillance - (food/fee 10 0 HACCP and own check - Objective sampling d) single 25 Gram N_A 50 0 detection Listeria monocytogenes (food/fee 50 0 d) Cheeses, made from mixed milk from cows, sheep and/or goats - soft and semi-soft - made single 1 Gram Only enumeration 5 0 <= 100 Listeria monocytogenes 5 0 from pasteurised milk - Processing plant - Bulgaria - food sample - milk - Surveillance - (food/fee Official sampling - Objective sampling d) >100 Listeria monocytogenes 5 0 Cheeses, made from mixed milk from cows, sheep and/or goats - soft and semi-soft - made batch 25 Gram N_A 115 0 detection Listeria monocytogenes from pasteurised milk - Processing plant - Bulgaria - food sample - milk - Surveillance - (food/fee 115 0 Official sampling - Objective sampling d) single 25 Gram Only detection 50 0 detection Listeria monocytogenes (food/fee 50 0 d) Cheeses, made from mixed milk from cows, sheep and/or goats - soft and semi-soft - made single 1 Gram N_A 10 0 <= 100 Listeria monocytogenes 10 0 from pasteurised milk - Retail - Bulgaria - food sample - milk - Surveillance - Official sampling (food/fee - Objective sampling d) >100 Listeria monocytogenes 10 0 Cheeses, made from mixed milk from cows, sheep and/or goats - soft and semi-soft - single 25 Gram N_A 13 0 detection Listeria monocytogenes Processing plant - Not Available - Not Available - Surveillance - based on Regulation 2073 - (food/fee 13 0 Official sampling - Objective sampling d) Cheeses, made from mixed milk from cows, sheep and/or goats - unspecified - Processing batch 25 Gram N_A 2512 0 detection Listeria monocytogenes plant - Not Available - Not Available - Surveillance - HACCP and own check - Objective (food/fee 2,512 0 sampling d) Cheeses, made from unspecified milk or other animal milk - fresh - Processing plant - Not single 25 Gram N_A 12 0 detection Listeria monocytogenes Available - Not Available - Surveillance - based on Regulation 2073 - HACCP and own check (food/fee 12 0 - Objective sampling d) Cheeses, made from unspecified milk or other animal milk - fresh - Processing plant - Not single 25 Gram N_A 2 0 detection Listeria monocytogenes Available - Not Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 2 0 Objective sampling d) Cheeses, made from unspecified milk or other animal milk - hard - Retail - Not Available - single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Cheeses, made from unspecified milk or other animal milk - soft and semi-soft - made from single 25 Gram N_A 1 0 detection Listeria monocytogenes pasteurised milk - Processing plant - Bulgaria - food sample - Surveillance - based on (food/fee 1 0 Regulation 2073 - Official sampling - Objective sampling d) Chocolate - Processing plant - Bulgaria - food sample - Surveillance - Official sampling - single 25 Gram N_A 1 0 detection Listeria monocytogenes Objective sampling (food/fee 1 0 d) Cocoa and cocoa preparations, coffee and tea - Processing plant - Bulgaria - food sample - batch 25 Gram N_A 2 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 2 0 d)

Bulgaria - 2019 24 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Cocoa and cocoa preparations, coffee and tea - Processing plant - Bulgaria - food sample - batch 25 Gram N_A 1 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 1 0 d) Confectionery products and pastes - chocolate-based product - Processing plant - Bulgaria - single 25 Gram N_A 2 0 detection Listeria monocytogenes food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 2 0 sampling d) Confectionery products and pastes - chocolate-based product - Processing plant - Bulgaria - single 25 Gram N_A 13 0 detection Listeria monocytogenes food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 13 0 d) Confectionery products and pastes - chocolate-based product - Processing plant - Bulgaria - single 25 Gram N_A 11 0 detection Listeria monocytogenes 11 0 food sample - Surveillance - Official sampling - Objective sampling (food/fee d) 13 0 detection Listeria monocytogenes 13 0 Confectionery products and pastes - chocolate-based product - Processing plant - Not single 1 Gram only enumeration 2 0 <= 100 Listeria monocytogenes 2 0 Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 2 0 Confectionery products and pastes - chocolate-based product - Processing plant - Not single 25 Gram only detection 10 0 detection Listeria monocytogenes Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee 10 0 d) Confectionery products and pastes - chocolate-based product - Restaurant or Cafe or Pub or single 10 Gram N_A 18 0 <= 100 Listeria monocytogenes 18 0 Bar or Hotel or Catering service - Bulgaria - food sample - Surveillance - based on Regulation (food/fee 2073 - Official sampling - Objective sampling d) >100 Listeria monocytogenes 18 0 Confectionery products and pastes - chocolate-based product - Retail - Bulgaria - food batch 25 Gram N_A 3 0 detection Listeria monocytogenes sample - Surveillance - Official sampling - Objective sampling (food/fee 3 0 d) Confectionery products and pastes - chocolate-based product - Retail - Not Available - food single 1 Gram N_A 7 0 <= 100 Listeria monocytogenes 7 0 sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 7 0 Crustaceans - shrimps - shelled, shucked and cooked - Processing plant - Not Available - single 25 Gram N_A 40 0 detection Listeria monocytogenes food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 40 0 sampling d) Crustaceans - shrimps - shelled, shucked and cooked - Retail - Not Available - food sample - single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Crustaceans - shrimps - shelled, shucked and cooked - Retail - Not Available - food sample - single 25 Gram N_A 5 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 5 0 d) Dairy products (excluding cheeses) - butter - Border Control Posts - Serbia - food sample - single 25 Gram N_A 1 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 1 0 d) Dairy products (excluding cheeses) - butter - made from pasteurised milk - Processing plant - single 25 Gram N_A 55 0 detection Listeria monocytogenes Bulgaria - food sample - milk - Surveillance - HACCP and own check - Objective sampling (food/fee 55 0 d) Dairy products (excluding cheeses) - butter - made from pasteurised milk - Processing plant - single 25 Gram N_A 5 0 detection Listeria monocytogenes Bulgaria - food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 5 0 d) Dairy products (excluding cheeses) - butter - made from pasteurised milk - Processing plant - single 25 Gram N_A 15 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 15 0 Objective sampling d) Dairy products (excluding cheeses) - butter - made from pasteurised milk - Processing plant - single 25 Gram N_A 5 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 5 0 d) Dairy products (excluding cheeses) - butter - made from pasteurised milk - Processing plant - batch 25 Gram N_A 20 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - Official sampling - Objective sampling (food/fee 20 0 d) Dairy products (excluding cheeses) - butter - made from pasteurised milk - Processing plant - single 25 Gram N_A 20 0 detection Listeria monocytogenes Not Available - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 20 0 Objective sampling d) Dairy products (excluding cheeses) - butter - made from pasteurised milk - Processing plant - single 25 Gram N_A 35 0 detection Listeria monocytogenes Not Available - Not Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 35 0 Objective sampling d) Dairy products (excluding cheeses) - butter - made from pasteurised milk - Retail - Bulgaria - single 25 Gram N_A 10 0 detection Listeria monocytogenes food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 10 0 d) Dairy products (excluding cheeses) - butter - made from pasteurised milk - Retail - Bulgaria - single 1 Gram N_A 40 0 <= 100 Listeria monocytogenes 40 0 food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee sampling d) >100 Listeria monocytogenes 40 0

Bulgaria - 2019 25 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Dairy products (excluding cheeses) - butter - made from pasteurised milk - Retail - Not single 1 Gram N_A 20 0 <= 100 Listeria monocytogenes 20 0 Available - Not Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Objective sampling d) >100 Listeria monocytogenes 20 0 Dairy products (excluding cheeses) - butter - Processing plant - Bulgaria - food sample - milk - single 25 Gram N_A 13 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 13 0 d) Dairy products (excluding cheeses) - butter - Processing plant - Bulgaria - food sample - milk - single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Dairy products (excluding cheeses) - butter - Processing plant - Bulgaria - food sample - single 25 Gram N_A 5 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 5 0 d) Dairy products (excluding cheeses) - butter - Processing plant - Bulgaria - food sample - single 25 Gram N_A 2 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 2 0 d) Dairy products (excluding cheeses) - butter - Processing plant - Not Available - Not Available - single 25 Gram N_A 15 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 15 0 d) Dairy products (excluding cheeses) - butter - Retail - Bulgaria - food sample - Surveillance - single 25 Gram N_A 4 0 detection Listeria monocytogenes based on Regulation 2073 - Official sampling - Objective sampling (food/fee 4 0 d) Dairy products (excluding cheeses) - buttermilk - Processing plant - Bulgaria - food sample - single 25 Millilitre N_A 35 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 35 0 d) Dairy products (excluding cheeses) - cheese analogue - Processing plant - Bulgaria - food single 1 Gram Only enumeration 15 0 <= 100 Listeria monocytogenes 15 0 sample - milk - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 15 0 Dairy products (excluding cheeses) - cheese analogue - Processing plant - Bulgaria - food single 25 Gram Only detection 65 0 detection Listeria monocytogenes sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 65 0 d) Dairy products (excluding cheeses) - cheese analogue - Processing plant - Bulgaria - food single 25 Gram N_A 3 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 3 0 d) Dairy products (excluding cheeses) - cheese analogue - Processing plant - Not Available - single 25 Gram N_A 25 0 detection Listeria monocytogenes food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 25 0 sampling d) Dairy products (excluding cheeses) - cheese analogue - Retail - Bulgaria - food sample - single 25 Gram N_A 1 1 detection Listeria monocytogenes O1/2 1 1 Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) 4 0 detection Listeria monocytogenes 4 0 Dairy products (excluding cheeses) - cheese analogue - Retail - Not Available - food sample - single 1 Gram only enumeration 15 0 <= 100 Listeria monocytogenes 15 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 15 0 Dairy products (excluding cheeses) - cheese analogue - Retail - Not Available - food sample - single 25 Gram only detection 25 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 25 0 d) Dairy products (excluding cheeses) - cream - Processing plant - Bulgaria - food sample - milk single 1 Gram Only enumeration 5 0 <= 100 Listeria monocytogenes 5 0 - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Dairy products (excluding cheeses) - cream - Processing plant - Bulgaria - food sample - milk single 25 Gram Only detection 60 0 detection Listeria monocytogenes - Surveillance - Official sampling - Objective sampling (food/fee 60 0 d) Dairy products (excluding cheeses) - cream - Processing plant - Bulgaria - food sample - single 25 Gram N_A 10 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Dairy products (excluding cheeses) - dairy desserts - Catering - Not Available - food sample - single 25 Gram N_A 24 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 24 0 d) Dairy products (excluding cheeses) - dairy desserts - Processing plant - Not Available - Not single 25 Gram N_A 4 0 detection Listeria monocytogenes Available - Surveillance - based on Regulation 2073 - HACCP and own check - Objective (food/fee 4 0 sampling d) Dairy products (excluding cheeses) - dairy desserts - Processing plant - Not Available - Not single 25 Gram N_A 13 0 detection Listeria monocytogenes Available - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 13 0 d) Dairy products (excluding cheeses) - dairy products, not specified - made from pasteurised single 25 Gram N_A 10 0 detection Listeria monocytogenes milk - Processing plant - Not Available - Not Available - Surveillance - based on Regulation (food/fee 10 0 2073 - Official sampling - Objective sampling d)

Bulgaria - 2019 26 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Dairy products (excluding cheeses) - dairy products, not specified - ready-to-eat - made from single 25 Gram N_A 80 0 detection Listeria monocytogenes pasteurised milk - Processing plant - Bulgaria - food sample - milk - Surveillance - HACCP (food/fee 80 0 and own check - Objective sampling d) Dairy products (excluding cheeses) - dairy products, not specified - ready-to-eat - made from single 25 Gram N_A 105 0 detection Listeria monocytogenes pasteurised milk - Processing plant - Bulgaria - food sample - milk - Surveillance - Official (food/fee 105 0 sampling - Objective sampling d) Dairy products (excluding cheeses) - dairy products, not specified - ready-to-eat - made from single 25 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 pasteurised milk - Retail - Bulgaria - food sample - milk - Surveillance - Official sampling - (food/fee Objective sampling d) >100 Listeria monocytogenes 5 0 Dairy products (excluding cheeses) - dairy products, not specified - ready-to-eat - Restaurant single 25 Gram N_A 23 0 detection Listeria monocytogenes or Cafe or Pub or Bar or Hotel or Catering service - Not Available - Not Available - (food/fee 23 0 Surveillance - based on Regulation 2073 - Official sampling - Objective sampling d) Dairy products (excluding cheeses) - fermented dairy products - fermented cream - single 25 Gram N_A 2 0 detection Listeria monocytogenes Processing plant - Bulgaria - food sample - Surveillance - HACCP and own check - Objective (food/fee 2 0 sampling d) Dairy products (excluding cheeses) - fermented dairy products - fermented milk - Border single 25 Gram N_A 1 0 detection Listeria monocytogenes Control Posts - Serbia - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1 0 sampling - Objective sampling d) Dairy products (excluding cheeses) - fermented dairy products - fermented milk - Processing single 25 Gram N_A 10 0 detection Listeria monocytogenes plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 10 0 Objective sampling d) Dairy products (excluding cheeses) - ice-cream - made from pasteurised milk - Processing single 25 Gram N_A 5 0 detection Listeria monocytogenes plant - Bulgaria - food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 5 0 d) Dairy products (excluding cheeses) - ice-cream - made from pasteurised milk - Processing single 25 Gram N_A 45 0 detection Listeria monocytogenes plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 45 0 Objective sampling d) Dairy products (excluding cheeses) - ice-cream - made from pasteurised milk - Processing single 1 Gram N_A 300 0 <= 100 Listeria monocytogenes 300 0 plant - Bulgaria - food sample - Surveillance - HACCP and own check - Objective sampling (food/fee d) >100 Listeria monocytogenes 300 0 Dairy products (excluding cheeses) - ice-cream - made from pasteurised milk - Processing single 25 Gram N_A 25 0 detection Listeria monocytogenes plant - Not Available - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 25 0 sampling - Objective sampling d) Dairy products (excluding cheeses) - ice-cream - made from pasteurised milk - Processing single 25 Gram N_A 2 0 detection Listeria monocytogenes plant - Not Available - Not Available - Surveillance - based on Regulation 2073 - HACCP and (food/fee 2 0 own check - Objective sampling d) Dairy products (excluding cheeses) - ice-cream - made from pasteurised milk - Processing single 25 Gram N_A 2 0 detection Listeria monocytogenes plant - Not Available - Not Available - Surveillance - based on Regulation 2073 - Official (food/fee 2 0 sampling - Objective sampling d) Dairy products (excluding cheeses) - ice-cream - Processing plant - Bulgaria - food sample - single 1 Gram only enumeration 9 0 <= 100 Listeria monocytogenes 9 0 milk - Surveillance - HACCP and own check - Objective sampling (food/fee d) >100 Listeria monocytogenes 9 0 Dairy products (excluding cheeses) - ice-cream - Processing plant - Bulgaria - food sample - single 25 Gram only detection 16 0 detection Listeria monocytogenes milk - Surveillance - HACCP and own check - Objective sampling (food/fee 16 0 d) Dairy products (excluding cheeses) - ice-cream - Processing plant - Bulgaria - food sample - single 25 Gram N_A 1 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 1 0 d) Dairy products (excluding cheeses) - ice-cream - Processing plant - Not Available - Not single 25 Gram N_A 12 0 detection Listeria monocytogenes Available - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 12 0 d) Dairy products (excluding cheeses) - ice-cream - Processing plant - Not Available - Not single 25 Gram N_A 12 0 detection Listeria monocytogenes Available - Surveillance - HACCP and own check - Objective sampling (food/fee 12 0 d) Dairy products (excluding cheeses) - milk-based drinks - Processing plant - Not Available - single 25 Millilitre N_A 5 0 detection Listeria monocytogenes food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 5 0 sampling d) Dairy products (excluding cheeses) - milk-based drinks - Retail - Not Available - food sample - single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Dairy products (excluding cheeses) - sour milk - Processing plant - Bulgaria - food sample - single 25 Gram N_A 16 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 16 0 d) Dairy products (excluding cheeses) - sour milk - Processing plant - Bulgaria - food sample - single 25 Gram N_A 23 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 23 0 d)

Bulgaria - 2019 27 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Dairy products (excluding cheeses) - sour milk - Processing plant - Not Available - food single 25 Millilitre N_A 20 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 20 0 d) Dairy products (excluding cheeses) - sour milk - Processing plant - Not Available - Not single 25 Gram N_A 5 0 detection Listeria monocytogenes Available - Surveillance - based on Regulation 2073 - HACCP and own check - Objective (food/fee 5 0 sampling d) Dairy products (excluding cheeses) - sour milk - Processing plant - Not Available - Not single 25 Gram N_A 10 0 detection Listeria monocytogenes Available - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Dairy products (excluding cheeses) - sour milk - Retail - Bulgaria - food sample - Surveillance single 25 Gram N_A 13 0 detection Listeria monocytogenes - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 13 0 d) Dairy products (excluding cheeses) - sour milk - Retail - Not Available - food sample - single 1 Gram N_A 60 0 <= 100 Listeria monocytogenes 60 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 60 0 Dairy products (excluding cheeses) - yoghurt - Processing plant - Bulgaria - food sample - single 25 Gram N_A 15 0 detection Listeria monocytogenes milk - Surveillance - HACCP and own check - Objective sampling (food/fee 15 0 d) Dairy products (excluding cheeses) - yoghurt - Processing plant - Bulgaria - food sample - single 1 Gram Only enumeration 35 0 <= 100 Listeria monocytogenes 35 0 milk - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 35 0 Dairy products (excluding cheeses) - yoghurt - Processing plant - Bulgaria - food sample - single 25 Gram Only detection 75 0 detection Listeria monocytogenes milk - Surveillance - Official sampling - Objective sampling (food/fee 75 0 d) Dairy products (excluding cheeses) - yoghurt - Processing plant - Bulgaria - food sample - single 25 Gram N_A 50 0 >100 Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 50 0 d) Dairy products (excluding cheeses) - yoghurt - Processing plant - Bulgaria - food sample - batch 25 Gram N_A 35 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 35 0 d) Dairy products (excluding cheeses) - yoghurt - Processing plant - Bulgaria - food sample - batch 25 Gram N_A 175 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 175 0 d) single 25 Gram N_A 15 0 detection Listeria monocytogenes (food/fee 15 0 d) Dairy products (excluding cheeses) - yoghurt - Processing plant - Not Available - Not single 25 Gram N_A 23 0 detection Listeria monocytogenes Available - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 23 0 d) Dairy products (excluding cheeses) - yoghurt - Processing plant - Not Available - Not single 25 Gram N_A 4 0 detection Listeria monocytogenes Available - Surveillance - HACCP and own check - Objective sampling (food/fee 4 0 d) Dairy products (excluding cheeses) - yoghurt - Retail - Bulgaria - food sample - Surveillance - single 1 Gram N_A 55 0 <= 100 Listeria monocytogenes 55 0 based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 55 0 Dairy products, unspecified - Processing plant - Bulgaria - food sample - Surveillance - based single 25 Gram N_A 15 0 detection Listeria monocytogenes on Regulation 2073 - Official sampling - Objective sampling (food/fee 15 0 d) Fish - gravad /slightly salted - Processing plant - Bulgaria - food sample - Surveillance - based single 25 Gram N_A 10 0 detection Listeria monocytogenes on Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Fish - gravad /slightly salted - Retail - Bulgaria - food sample - Surveillance - Official sampling single 1 Gram Only enumeration 10 0 <= 100 Listeria monocytogenes 10 0 - Objective sampling (food/fee d) >100 Listeria monocytogenes 10 0 Fish - marinated - Processing plant - Bulgaria - food sample - Surveillance - based on single 10 Gram only enumeration 1 0 <= 100 Listeria monocytogenes 1 0 Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 1 0 Fish - marinated - Processing plant - Bulgaria - food sample - Surveillance - based on single 25 Gram only detection 80 0 detection Listeria monocytogenes Regulation 2073 - Official sampling - Objective sampling (food/fee 80 0 d) Fish - marinated - Processing plant - Bulgaria - food sample - Surveillance - HACCP and own single 25 Gram N_A 60 0 detection Listeria monocytogenes check - Objective sampling (food/fee 60 0 d) Fish - marinated - Processing plant - Not Available - food sample - Surveillance - based on single 25 Gram N_A 55 0 detection Listeria monocytogenes Regulation 2073 - Official sampling - Objective sampling (food/fee 55 0 d)

Bulgaria - 2019 28 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Fish - marinated - Retail - Bulgaria - food sample - Surveillance - based on Regulation 2073 - single 1 Gram only enumeration 65 0 <= 100 Listeria monocytogenes 65 0 Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 65 0 Fish - marinated - Retail - Bulgaria - food sample - Surveillance - based on Regulation 2073 - single 25 Gram only detection 30 0 detection Listeria monocytogenes Official sampling - Objective sampling (food/fee 30 0 d) Fish - marinated - Retail - Bulgaria - food sample - Surveillance - Official sampling - Objective single 1 Gram N_A 10 0 <= 100 Listeria monocytogenes 10 0 sampling (food/fee d) >100 Listeria monocytogenes 10 0 Fish - marinated - Retail - Not Available - food sample - Surveillance - Official sampling - single 1 Gram only enumeration 155 0 <= 100 Listeria monocytogenes 155 0 Objective sampling (food/fee d) >100 Listeria monocytogenes 155 0 Fish - marinated - Retail - Not Available - food sample - Surveillance - Official sampling - single 25 Gram only detection 110 0 detection Listeria monocytogenes Objective sampling (food/fee 110 0 d) Fish - raw - chilled - Processing plant - Bulgaria - food sample - Surveillance - Official single 25 Gram N_A 5 0 detection Listeria monocytogenes sampling - Objective sampling (food/fee 5 0 d) Fish - raw - Processing plant - Not Available - Not Available - Surveillance - HACCP and own single 25 Gram N_A 3 0 detection Listeria monocytogenes check - Objective sampling (food/fee 3 0 d) Fish - smoked - Border Control Posts - Not Available - Not Available - Surveillance - Official batch 25 Gram N_A 6 0 detection Listeria monocytogenes sampling - Objective sampling (food/fee 6 0 d) Fish - smoked - cold-smoked - Processing plant - Bulgaria - food sample - Surveillance - single 25 Gram N_A 6 6 detection Listeria monocytogenes O1/2 based on Regulation 2073 - Official sampling - Objective sampling (food/fee 6 6 d) Fish - smoked - cold-smoked - Processing plant - Bulgaria - food sample - Surveillance - batch 25 Gram N_A 15 0 detection Listeria monocytogenes HACCP and own check - Objective sampling (food/fee 15 0 d) Fish - smoked - cold-smoked - Processing plant - Ireland - food sample - Surveillance - based single 25 Gram N_A 5 5 detection Listeria grayi 5 5 on Regulation 2073 - Official sampling - Objective sampling (food/fee d) Listeria monocytogenes O1/2 5 5 Fish - smoked - cold-smoked - Retail - Bulgaria - food sample - Surveillance - based on single 25 Gram N_A 1 1 detection Listeria monocytogenes O1/2 Regulation 2073 - Official sampling - Objective sampling (food/fee 1 1 d) Fish - smoked - hot-smoked - Processing plant - Bulgaria - food sample - Surveillance - batch 25 Gram N_A 10 1 detection Listeria monocytogenes 10 0 HACCP and own check - Objective sampling (food/fee d) Listeria monocytogenes O1/2 10 1 Fish - smoked - hot-smoked - Retail - Bulgaria - food sample - Surveillance - based on single 1 Gram N_A 5 5 <= 100 Listeria monocytogenes 5 5 Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Fish - smoked - hot-smoked - Retail - Bulgaria - food sample - Surveillance - Official sampling single 25 Gram N_A 20 0 detection Listeria monocytogenes - Objective sampling (food/fee 20 0 d) Fish - smoked - Processing plant - Bulgaria - food sample - Surveillance - based on single 25 Gram N_A 4 4 detection Listeria monocytogenes O1/2 4 4 Regulation 2073 - Official sampling - Objective sampling (food/fee d) 52 0 detection Listeria monocytogenes 52 0 Fish - smoked - Processing plant - Bulgaria - food sample - Surveillance - HACCP and own batch 1 Millilitre N_A 1 0 detection Listeria monocytogenes check - Objective sampling (food/fee 1 0 d) Fish - smoked - Processing plant - Not Available - food sample - Surveillance - based on single 25 Gram N_A 25 0 detection Listeria monocytogenes Regulation 2073 - Official sampling - Objective sampling (food/fee 25 0 d) Fish - smoked - Retail - Bulgaria - food sample - Surveillance - based on Regulation 2073 - single 1 Gram only enumeration 60 0 <= 100 Listeria monocytogenes 60 0 Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 60 0 Fish - smoked - Retail - Bulgaria - food sample - Surveillance - based on Regulation 2073 - single 25 Gram only detection 23 6 detection Listeria monocytogenes - 23 1 Official sampling - Objective sampling (food/fee serovar 4b d) Listeria monocytogenes O1/2 23 5 Fish - smoked - Retail - Bulgaria - food sample - Surveillance - Official sampling - Objective single 1 Gram N_A 10 0 <= 100 Listeria monocytogenes 10 0 sampling (food/fee d) >100 Listeria monocytogenes 10 0 Fish - smoked - Retail - Not Available - food sample - Surveillance - Official sampling - single 1 Gram N_A 20 0 <= 100 Listeria monocytogenes 20 0 Objective sampling (food/fee d) >100 Listeria monocytogenes 20 0

Bulgaria - 2019 29 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Fish - unspecified - Processing plant - Not Available - Not Available - Surveillance - HACCP batch 25 Gram N_A 130 0 detection Listeria monocytogenes and own check - Objective sampling (food/fee 130 0 d) Fishery products, unspecified - cooked - Processing plant - Not Available - Not Available - single 25 Gram N_A 11 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 11 0 d) Fishery products, unspecified - ready-to-eat - chilled - Processing plant - Not Available - Not single 25 Gram N_A 7 0 detection Listeria monocytogenes Available - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 7 0 d) Fishery products, unspecified - ready-to-eat - chilled - Processing plant - Not Available - Not single 25 Gram N_A 6 0 detection Listeria monocytogenes Available - Surveillance - HACCP and own check - Objective sampling (food/fee 6 0 d) Fishery products, unspecified - ready-to-eat - Processing plant - Not Available - Not Available single 25 Gram N_A 12 0 detection Listeria monocytogenes - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 12 0 d) Fishery products, unspecified - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - single 1 Gram only enumeration 40 0 <= 100 Listeria monocytogenes 40 0 based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 40 0 Fishery products, unspecified - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - single 25 Gram only detection 25 0 detection Listeria monocytogenes based on Regulation 2073 - Official sampling - Objective sampling (food/fee 25 0 d) Fishery products, unspecified - ready-to-eat - Retail - Not Available - food sample - single 1 Gram only enumeration 5 0 <= 100 Listeria monocytogenes 5 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Fishery products, unspecified - ready-to-eat - Retail - Not Available - food sample - single 25 Gram only detection 10 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 10 0 d) Fishery products, unspecified - smoked - Processing plant - Not Available - Not Available - single 25 Gram N_A 9 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 9 0 d) Foodstuffs intended for special nutritional uses - dietary foods for special medical purposes - batch 25 Gram N_A 7 0 detection Listeria monocytogenes Processing plant - Bulgaria - food sample - Surveillance - HACCP and own check - Objective (food/fee 7 0 sampling d) Foodstuffs intended for special nutritional uses - ready-to-eat - Processing plant - Bulgaria - single 25 Gram N_A 3 0 detection Listeria monocytogenes food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 3 0 sampling d) Foodstuffs intended for special nutritional uses - ready-to-eat - Processing plant - Bulgaria - single 25 Gram N_A 4 0 detection Listeria monocytogenes food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 4 0 d) Fruits - non-pre-cut - Retail - Not Available - food sample - Surveillance - Official sampling - single 1 Gram N_A 1 0 <= 100 Listeria monocytogenes 1 0 Objective sampling (food/fee d) >100 Listeria monocytogenes 1 0 Fruits - pre-cut - ready-to-eat - Catering - Bulgaria - food sample - Surveillance - based on single 25 Gram N_A 2 0 detection Listeria monocytogenes Regulation 2073 - Official sampling - Objective sampling (food/fee 2 0 d) Fruits - products - dried - Processing plant - Bulgaria - food sample - Surveillance - based on single 25 Gram N_A 1 0 detection Listeria monocytogenes Regulation 2073 - Official sampling - Objective sampling (food/fee 1 0 d) Fruits - products - dried - Processing plant - Bulgaria - food sample - Surveillance - HACCP single 25 Gram N_A 2 0 detection Listeria monocytogenes and own check - Objective sampling (food/fee 2 0 d) Fruits - products - Retail - Bulgaria - food sample - Surveillance - based on Regulation 2073 - single 1 Millilitre N_A 40 0 detection Listeria monocytogenes Official sampling - Objective sampling (food/fee 40 0 d) Juice - fruit juice - pasteurised - Processing plant - Bulgaria - food sample - Surveillance - single 25 Gram N_A 1 0 detection Listeria monocytogenes based on Regulation 2073 - Official sampling - Objective sampling (food/fee 1 0 d) Juice - fruit juice - unpasteurised - Restaurant or Cafe or Pub or Bar or Hotel or Catering single 25 Gram N_A 5 0 detection Listeria monocytogenes 5 0 service - Not Available - Not Available - Surveillance - based on Regulation 2073 - Official (food/fee sampling - Objective sampling d) 7 0 detection Listeria monocytogenes 7 0 Juice - fruit juice - unpasteurised - Retail - Bulgaria - food sample - Surveillance - based on single 10 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Juice - fruit juice - unpasteurised - Retail - Bulgaria - food sample - Surveillance - Official batch 25 Gram N_A 15 0 detection Listeria monocytogenes sampling - Objective sampling (food/fee 15 0 d)

Bulgaria - 2019 30 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Juice - fruit juice - unpasteurised - Retail - Bulgaria - food sample - Surveillance - Official single 25 Gram N_A 30 0 detection Listeria monocytogenes sampling - Objective sampling (food/fee 30 0 d) Juice - fruit juice - unpasteurised - Retail - Not Available - food sample - Surveillance - Official single 1 Gram N_A 16 0 <= 100 Listeria monocytogenes 16 0 sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 16 0 Juice - mixed juice - unpasteurised - Restaurant or Cafe or Pub or Bar or Hotel or Catering single 1 Millilitre N_A 20 0 <= 100 Listeria monocytogenes 20 0 service - Bulgaria - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 20 0 Low alcoholic beverage prepared by fermented cereals - Processing plant - Bulgaria - food single 25 Gram N_A 3 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 3 0 d) Low alcoholic beverage prepared by fermented cereals - Processing plant - Bulgaria - food single 25 Gram N_A 4 0 detection Listeria monocytogenes 4 0 sample - Surveillance - Official sampling - Objective sampling (food/fee N_A d) Millilitre 25 0 detection Listeria monocytogenes 25 0 Low alcoholic beverage prepared by fermented cereals - Processing plant - Not Available - single 25 Millilitre N_A 3 0 detection Listeria monocytogenes food sample - Surveillance - Official sampling - Objective sampling (food/fee 3 0 d) Low alcoholic beverage prepared by fermented cereals - Retail - Bulgaria - food sample - single 10 Gram N_A 1 0 <= 100 Listeria monocytogenes 1 0 Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 1 0 Low alcoholic beverage prepared by fermented cereals - Retail - Bulgaria - food sample - single 25 Gram N_A 1 0 detection Listeria monocytogenes 1 0 Surveillance - Official sampling - Objective sampling (food/fee N_A d) Millilitre 10 0 detection Listeria monocytogenes 10 0 Low alcoholic beverage prepared by fermented cereals - Retail - Not Available - food sample - single 1 Gram N_A 2 0 <= 100 Listeria monocytogenes 2 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 2 0 Low alcoholic beverage prepared by fermented cereals - Retail - Not Available - food sample - single 25 Millilitre N_A 1 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 1 0 d) Meat from bovine animals - meat products - cooked, ready-to-eat - chilled - Processing plant - single 25 Gram N_A 12 0 detection Listeria monocytogenes Not Available - Not Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 12 0 Objective sampling d) Meat from bovine animals - meat products - cooked, ready-to-eat - chilled - Processing plant - single 25 Gram N_A 6 0 detection Listeria monocytogenes Not Available - Not Available - Surveillance - HACCP and own check - Objective sampling (food/fee 6 0 d) Meat from bovine animals - meat products - cooked, ready-to-eat - Processing plant - Bulgaria single 25 Gram N_A 140 0 detection Listeria monocytogenes - food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 140 0 sampling d) Meat from bovine animals - meat products - cooked, ready-to-eat - Processing plant - Not single 25 Gram N_A 5 0 detection Listeria monocytogenes Available - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 5 0 Objective sampling d) Meat from bovine animals - meat products - cooked, ready-to-eat - Retail - Bulgaria - food single 1 Gram only enumeration 255 0 <= 100 Listeria monocytogenes 255 0 sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 255 0 Meat from bovine animals - meat products - cooked, ready-to-eat - Retail - Bulgaria - food single 25 Gram only detection 45 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 45 0 d) Meat from bovine animals - meat products - cooked, ready-to-eat - Retail - Not Available - single 1 Gram N_A 10 0 <= 100 Listeria monocytogenes 10 0 food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 10 0 Meat from bovine animals - meat products - fermented sausages - Processing plant - Bulgaria single 25 Gram N_A 12 0 detection Listeria monocytogenes - food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 12 0 sampling d) Meat from bovine animals - meat products - fermented sausages - Processing plant - Bulgaria single 25 Gram N_A 11 0 detection Listeria monocytogenes - food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 11 0 d) Meat from bovine animals - meat products - fermented sausages - Retail - Bulgaria - food single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 sample - meat - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Meat from bovine animals - meat products - fermented sausages - Retail - Bulgaria - food single 25 Gram N_A 11 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 11 0 d) Meat from bovine animals - meat products - ready-to-eat - Processing plant - Not Available - single 25 Gram N_A 14 0 detection Listeria monocytogenes Not Available - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 14 0 sampling d)

Bulgaria - 2019 31 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Meat from bovine animals - meat products - unspecified, ready-to-eat - Processing plant - Not single 25 Gram N_A 3 0 detection Listeria monocytogenes Available - Not Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 3 0 Objective sampling d) Meat from bovine animals and pig - meat products - Game handling establishment - Not batch 200 Gram N_A 312 0 detection Listeria monocytogenes Available - Not Available - Surveillance - HACCP and own check - Objective sampling (food/fee 312 0 d) Meat from bovine animals and pig - meat products - ready-to-eat - Processing plant - Not single 25 Gram N_A 16 0 detection Listeria monocytogenes Available - Not Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 16 0 Objective sampling d) Meat from bovine animals and pig - meat products - ready-to-eat - Processing plant - Not single 25 Gram N_A 6 0 detection Listeria monocytogenes Available - Not Available - Surveillance - HACCP and own check - Objective sampling (food/fee 6 0 d) Meat from broilers (Gallus gallus) - fresh - chilled - Processing plant - Not Available - Not batch 25 Gram N_A 774 0 detection Listeria monocytogenes Available - Surveillance - HACCP and own check - Objective sampling (food/fee 774 0 d) Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - Processing plant - single 25 Gram N_A 15 0 detection Listeria monocytogenes Bulgaria - food sample - meat - Surveillance - HACCP and own check - Objective sampling (food/fee 15 0 d) Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - Processing plant - single 25 Gram N_A 20 0 detection Listeria monocytogenes Bulgaria - food sample - meat - Surveillance - Official sampling - Objective sampling (food/fee 20 0 d) Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - Processing plant - single 25 Gram N_A 10 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 10 0 Objective sampling d) Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - Processing plant - single 25 Gram N_A 30 0 detection Listeria monocytogenes Not Available - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 30 0 Objective sampling d) Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - Restaurant or Cafe single 1 Gram Only enumeration 30 0 <= 100 Listeria monocytogenes 30 0 or Pub or Bar or Hotel or Catering service - Bulgaria - food sample - meat - Surveillance - (food/fee based on Regulation 2073 - Official sampling - Objective sampling d) >100 Listeria monocytogenes 30 0 Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - Restaurant or Cafe single 25 Gram Only detection 10 0 detection Listeria monocytogenes or Pub or Bar or Hotel or Catering service - Bulgaria - food sample - meat - Surveillance - (food/fee 10 0 based on Regulation 2073 - Official sampling - Objective sampling d) Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - Retail - Bulgaria - single 25 Gram N_A 10 0 <= 100 Listeria monocytogenes 10 0 food sample - meat - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 10 0 Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - Retail - Bulgaria - single 1 Gram N_A 10 0 <= 100 Listeria monocytogenes 10 0 food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee sampling d) >100 Listeria monocytogenes 10 0 Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - Retail - Not single 1 Gram only enumeration 15 0 <= 100 Listeria monocytogenes 15 0 Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 15 0 Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - Retail - Not single 25 Gram only detection 20 0 detection Listeria monocytogenes Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee 20 0 d) Meat from duck - carcase - chilled - Processing plant - Not Available - Not Available - batch 25 Gram N_A 180 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 180 0 d) Meat from duck - meat products - raw but intended to be eaten cooked - chilled - Processing single 25 Gram N_A 3 0 detection Listeria monocytogenes plant - Not Available - Not Available - Surveillance - Official sampling - Objective sampling (food/fee 3 0 d) Meat from duck - offal - liver - chilled - Processing plant - Not Available - Not Available - single 25 Gram N_A 3 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 3 0 d) Meat from pig - meat preparation - intended to be eaten cooked - Processing plant - Not single 25 Gram N_A 10 0 detection Listeria monocytogenes Available - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 10 0 Objective sampling d) Meat from pig - meat preparation - intended to be eaten cooked - Retail - Not Available - food single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Meat from pig - meat products - cooked ham - Processing plant - Not Available - food sample single 25 Gram N_A 5 0 detection Listeria monocytogenes - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 5 0 d) Meat from pig - meat products - cooked ham - Processing plant - Not Available - Not Available single 25 Gram N_A 11 0 detection Listeria monocytogenes - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 11 0 d)

Bulgaria - 2019 32 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Meat from pig - meat products - cooked ham - Retail - Not Available - food sample - single 1 Gram only enumeration 10 0 <= 100 Listeria monocytogenes 10 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 10 0 Meat from pig - meat products - cooked ham - Retail - Not Available - food sample - single 25 Gram only detection 5 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 5 0 d) Meat from pig - meat products - cooked ham - sliced - Processing plant - Bulgaria - food batch 25 Gram N_A 2 2 detection Listeria monocytogenes O1/2 sample - Surveillance - based on Regulation 2073 - HACCP and own check - Objective (food/fee 2 2 sampling d) Meat from pig - meat products - cooked, ready-to-eat - chilled - Processing plant - Bulgaria - batch 25 Gram N_A 3 3 detection Listeria monocytogenes O1/2 food sample - Surveillance - based on Regulation 2073 - HACCP and own check - Objective (food/fee 3 3 sampling d) Meat from pig - meat products - cooked, ready-to-eat - chilled - Processing plant - Not single 25 Gram N_A 145 0 detection Listeria monocytogenes Available - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 145 0 Objective sampling d) Meat from pig - meat products - cooked, ready-to-eat - chilled - Retail - Not Available - food single 1 Gram only enumeration 120 0 <= 100 Listeria monocytogenes 120 0 sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 120 0 Meat from pig - meat products - cooked, ready-to-eat - chilled - Retail - Not Available - food single 25 Gram only detection 30 0 detection Listeria monocytogenes sample - Surveillance - Official sampling - Objective sampling (food/fee 30 0 d) Meat from pig - meat products - cooked, ready-to-eat - Processing plant - Bulgaria - food single 25 Gram N_A 63 5 detection Listeria monocytogenes 63 0 sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) Listeria monocytogenes O1/2 63 5 Meat from pig - meat products - cooked, ready-to-eat - Processing plant - Not Available - food single 25 Gram N_A 25 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 25 0 d) Meat from pig - meat products - cooked, ready-to-eat - Processing plant - Not Available - Not single 25 Gram N_A 12 0 detection Listeria monocytogenes Available - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 12 0 d) Meat from pig - meat products - cooked, ready-to-eat - Processing plant - Not Available - Not single 25 Gram N_A 8 0 detection Listeria monocytogenes Available - Surveillance - HACCP and own check - Objective sampling (food/fee 8 0 d) Meat from pig - meat products - cooked, ready-to-eat - Retail - Bulgaria - food sample - meat - single 1 Gram N_A 15 0 <= 100 Listeria monocytogenes 15 0 Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 15 0 Meat from pig - meat products - cooked, ready-to-eat - Retail - Bulgaria - food sample - meat - single 25 Gram N_A 15 0 <= 100 Listeria monocytogenes 15 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 15 0 Meat from pig - meat products - cooked, ready-to-eat - Retail - Bulgaria - food sample - single 1 Gram only enumeration 75 0 <= 100 Listeria monocytogenes 75 0 Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 75 0 Meat from pig - meat products - cooked, ready-to-eat - Retail - Bulgaria - food sample - single 25 Gram only detection 49 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 49 0 d) Meat from pig - meat products - cooked, ready-to-eat - Retail - Not Available - food sample - single 1 Gram only enumeration 35 0 <= 100 Listeria monocytogenes 35 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 35 0 Meat from pig - meat products - cooked, ready-to-eat - Retail - Not Available - food sample - single 25 Gram only detection 5 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 5 0 d) Meat from pig - meat products - fermented sausages - Processing plant - Bulgaria - food single 25 Gram N_A 68 0 detection Listeria monocytogenes sample - meat - Surveillance - Official sampling - Objective sampling (food/fee 68 0 d) Meat from pig - meat products - fermented sausages - Processing plant - Bulgaria - food single 25 Gram N_A 8 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 8 0 d) Meat from pig - meat products - fermented sausages - Processing plant - Bulgaria - food batch 1 Gram N_A 10 10 <= 100 Listeria monocytogenes 10 10 sample - Surveillance - HACCP and own check - Objective sampling (food/fee d) >100 Listeria monocytogenes 10 0 Meat from pig - meat products - fermented sausages - Processing plant - Bulgaria - food single 25 Gram N_A 12 0 detection Listeria monocytogenes sample - Surveillance - HACCP and own check - Objective sampling (food/fee 12 0 d) Meat from pig - meat products - fermented sausages - Retail - Bulgaria - food sample - meat - single 1 Gram N_A 52 0 <= 100 Listeria monocytogenes 52 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 52 0

Bulgaria - 2019 33 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Meat from pig - meat products - fermented sausages - Retail - Bulgaria - food sample - single 25 Gram N_A 10 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Meat from pig - meat products - fresh raw sausages - Retail - Bulgaria - food sample - single 25 Gram N_A 1 1 detection Listeria monocytogenes - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee serovar 7 1 1 d) Meat from pig - meat products - meat specialities - Processing plant - Bulgaria - food sample - single 1 Gram Only enumeration 10 0 <= 100 Listeria monocytogenes 10 0 meat - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 10 0 Meat from pig - meat products - meat specialities - Processing plant - Bulgaria - food sample - single 25 Gram Only detection 15 0 detection Listeria monocytogenes meat - Surveillance - Official sampling - Objective sampling (food/fee 15 0 d) Meat from pig - meat products - Processing plant - Not Available - food sample - Surveillance single 25 Gram N_A 35 0 detection Listeria monocytogenes - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 35 0 d) Meat from pig - meat products - ready-to-eat - Processing plant - Not Available - Not Available single 25 Gram N_A 10 0 detection Listeria monocytogenes - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Meat from pig - meat products - Retail - Not Available - food sample - Surveillance - Official single 1 Gram only enumeration 85 0 <= 100 Listeria monocytogenes 85 0 sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 85 0 Meat from pig - meat products - Retail - Not Available - food sample - Surveillance - Official single 25 Gram only detection 5 0 detection Listeria monocytogenes sampling - Objective sampling (food/fee 5 0 d) Meat from pig - meat products - unspecified, ready-to-eat - Processing plant - Bulgaria - food batch 25 Gram N_A 1 1 detection Listeria monocytogenes O4 sample - Surveillance - HACCP and own check - Objective sampling (food/fee 1 1 d) Meat from pig - meat products - unspecified, ready-to-eat - Processing plant - Not Available - single 25 Gram N_A 24 0 detection Listeria monocytogenes Not Available - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 24 0 sampling d) Meat from pig - meat products - unspecified, ready-to-eat - Retail - Not Available - Not single 1 Gram N_A 37 0 <= 100 Listeria monocytogenes 37 0 Available - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 37 0 Meat from poultry, unspecified - meat products - ready-to-eat - Processing plant - Not single 25 Gram N_A 15 0 detection Listeria monocytogenes Available - Not Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 15 0 Objective sampling d) Meat, mixed meat - meat preparation - Processing plant - Not Available - Not Available - batch 25 Gram N_A 2520 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 2,520 0 d) Meat, mixed meat - meat products - cooked, ready-to-eat - Processing plant - Bulgaria - food single 25 Gram only enumeration 80 0 <= 100 Listeria monocytogenes 80 0 sample - meat - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 80 0 Meat, mixed meat - meat products - cooked, ready-to-eat - Processing plant - Bulgaria - food single 25 Gram only detection 75 0 detection Listeria monocytogenes sample - meat - Surveillance - Official sampling - Objective sampling (food/fee 75 0 d) Meat, mixed meat - meat products - cooked, ready-to-eat - Processing plant - Bulgaria - food single 25 Gram N_A 20 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 20 0 d) Meat, mixed meat - meat products - cooked, ready-to-eat - Processing plant - Bulgaria - food batch 25 Gram N_A 45 0 detection Listeria monocytogenes sample - Surveillance - HACCP and own check - Objective sampling (food/fee 45 0 d) Meat, mixed meat - meat products - cooked, ready-to-eat - Processing plant - Bulgaria - food batch 25 Gram N_A 100 0 detection Listeria monocytogenes sample - Surveillance - Official sampling - Objective sampling (food/fee 100 0 d) Meat, mixed meat - meat products - cooked, ready-to-eat - Restaurant or Cafe or Pub or Bar single 1 Gram N_A 17 0 <= 100 Listeria monocytogenes 17 0 or Hotel or Catering service - Bulgaria - food sample - meat - Surveillance - Official sampling - (food/fee Objective sampling d) >100 Listeria monocytogenes 17 0 Meat, mixed meat - meat products - cooked, ready-to-eat - Retail - Bulgaria - food sample - single 25 Gram N_A 16 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 16 0 d) Meat, mixed meat - meat products - fermented sausages - Game handling establishment - batch 200 Gram N_A 243 0 detection Listeria monocytogenes Not Available - Not Available - Surveillance - HACCP and own check - Objective sampling (food/fee 243 0 d) Meat, mixed meat - meat products - fermented sausages - Processing plant - Bulgaria - food single 1 Gram Only enumeration 5 0 <= 100 Listeria monocytogenes 5 0 sample - meat - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0

Bulgaria - 2019 34 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Meat, mixed meat - meat products - fermented sausages - Processing plant - Bulgaria - food single 25 Gram Only detection 30 0 detection Listeria monocytogenes sample - meat - Surveillance - Official sampling - Objective sampling (food/fee 30 0 d) Meat, mixed meat - meat products - fermented sausages - Processing plant - Bulgaria - food single 25 Gram N_A 20 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 20 0 d) Meat, mixed meat - meat products - fermented sausages - Processing plant - Bulgaria - food batch 25 Gram N_A 45 0 detection Listeria monocytogenes sample - Surveillance - HACCP and own check - Objective sampling (food/fee 45 0 d) single 25 Gram N_A 32 0 detection Listeria monocytogenes (food/fee 32 0 d) Meat, mixed meat - meat products - fermented sausages - Processing plant - Bulgaria - food batch 25 Gram N_A 95 0 detection Listeria monocytogenes sample - Surveillance - Official sampling - Objective sampling (food/fee 95 0 d) Meat, mixed meat - meat products - fermented sausages - Retail - Bulgaria - food sample - single 25 Gram N_A 5 0 detection Listeria monocytogenes meat - Surveillance - Official sampling - Objective sampling (food/fee 5 0 d) Meat, mixed meat - meat products - fermented sausages - Retail - Bulgaria - food sample - single 25 Gram N_A 24 1 detection Listeria monocytogenes - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee serovar 4ab 24 1 d) Meat, mixed meat - meat products - fresh raw sausages - Processing plant - Bulgaria - food single 25 Gram N_A 15 0 detection Listeria monocytogenes sample - meat - Surveillance - Official sampling - Objective sampling (food/fee 15 0 d) Meat, mixed meat - meat products - ready-to-eat - Processing plant - Bulgaria - food sample - batch 1 Gram N_A 5 5 <= 100 Listeria monocytogenes 5 5 Surveillance - HACCP and own check - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Meat, mixed meat - minced meat - Retail - Bulgaria - food sample - Surveillance - Official batch 1 Gram only enumeration 2 0 <= 100 Listeria monocytogenes 2 0 sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 2 0 Meat, mixed meat - minced meat - Retail - Bulgaria - food sample - Surveillance - Official batch 25 Gram only detection 5 0 detection Listeria monocytogenes sampling - Objective sampling (food/fee 5 0 d) Milk, cows' - pasteurised milk - Game handling establishment - Not Available - Not Available - batch 200 Gram N_A 314 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 314 0 d) Milk, cows' - pasteurised milk - Processing plant - Bulgaria - food sample - milk - Surveillance batch 25 Gram N_A 10 0 detection Listeria monocytogenes - HACCP and own check - Objective sampling (food/fee 10 0 d) Milk, cows' - pasteurised milk - Processing plant - Bulgaria - food sample - milk - Surveillance batch 25 Gram N_A 5 0 detection Listeria monocytogenes - Official sampling - Objective sampling (food/fee 5 0 d) single 25 Millilitre N_A 5 0 detection Listeria monocytogenes 5 0 (food/fee d) 15 0 detection Listeria monocytogenes 15 0 Milk, cows' - pasteurised milk - Processing plant - Bulgaria - food sample - Surveillance - single 25 Millilitre N_A 25 0 detection Listeria monocytogenes based on Regulation 2073 - Official sampling - Objective sampling (food/fee 25 0 d) Milk, cows' - pasteurised milk - Processing plant - Not Available - food sample - Surveillance - single 25 Millilitre N_A 5 0 detection Listeria monocytogenes based on Regulation 2073 - Official sampling - Objective sampling (food/fee 5 0 d) Milk, cows' - pasteurised milk - Retail - Bulgaria - food sample - Surveillance - based on single 25 Gram N_A 2 0 detection Listeria monocytogenes Regulation 2073 - Official sampling - Objective sampling (food/fee 2 0 d) Milk, cows' - raw milk - Farm - Bulgaria - food sample - milk - Unspecified - Private sampling - single 1 Millilitre N_A 2 0 detection Listeria monocytogenes Convenient sampling (food/fee 2 0 d) Milk, cows' - raw milk for manufacture - intended for manufacture of pasteurised/UHT single 25 Gram N_A 5 0 detection Listeria monocytogenes products - Processing plant - Bulgaria - food sample - milk - Surveillance - based on (food/fee 5 0 Regulation 2073 - Official sampling - Objective sampling d) Milk, cows' - UHT milk - Processing plant - Bulgaria - food sample - milk - Surveillance - single 1 Millilitre N_A 5 0 <= 100 Listeria monocytogenes 5 0 Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Milk, cows' - UHT milk - Processing plant - Bulgaria - food sample - Surveillance - HACCP single 25 Gram N_A 4 0 detection Listeria monocytogenes and own check - Objective sampling (food/fee 4 0 d)

Bulgaria - 2019 35 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Milk, cows' - UHT milk - Retail - Bulgaria - food sample - Surveillance - based on Regulation single 25 Gram N_A 1 0 detection Listeria monocytogenes 2073 - Official sampling - Objective sampling (food/fee 1 0 d) Molluscan shellfish - cooked - frozen - Processing plant - Bulgaria - food sample - single 25 Gram N_A 43 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 43 0 d) Molluscan shellfish - raw - Processing plant - Bulgaria - food sample - Surveillance - based on single 25 Gram N_A 10 0 detection Listeria monocytogenes Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Molluscan shellfish - shelled, shucked and cooked - frozen - Processing plant - Not Available - single 25 Gram N_A 20 0 detection Listeria monocytogenes food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 20 0 sampling d) Other processed food products and prepared dishes - Catering - Bulgaria - food sample - single 25 Gram N_A 65 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 65 0 d) Other processed food products and prepared dishes - Catering - Bulgaria - food sample - single 25 Gram N_A 2 0 detection Listeria monocytogenes Surveillance - based on Regulation 2073 - Official sampling - Suspect sampling (food/fee 2 0 d) Other processed food products and prepared dishes - Catering - Bulgaria - food sample - single 25 Gram N_A 12 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 12 0 d) Other processed food products and prepared dishes - fish and seafood based dishes - Retail - batch 25 Gram N_A 10 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - Official sampling - Objective sampling (food/fee 10 0 d) Other processed food products and prepared dishes - meat based dishes - Catering - Not single 1 Gram only enumeration 6 0 <= 100 Listeria monocytogenes 6 0 Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 6 0 Other processed food products and prepared dishes - meat based dishes - Catering - Not single 25 Gram only detection 35 0 detection Listeria monocytogenes Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee 35 0 d) Other processed food products and prepared dishes - meat based dishes - Processing plant - single 25 Gram N_A 7 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 7 0 Objective sampling d) Other processed food products and prepared dishes - meat based dishes - Restaurant or single 1 Gram N_A 3 0 <= 100 Listeria monocytogenes 3 0 Cafe or Pub or Bar or Hotel or Catering service - Not Available - food sample - Surveillance - (food/fee Official sampling - Objective sampling d) >100 Listeria monocytogenes 3 0 Other processed food products and prepared dishes - meat based dishes - Restaurant or single 25 Gram N_A 48 0 detection Listeria monocytogenes Cafe or Pub or Bar or Hotel or Catering service - Not Available - Not Available - Surveillance - (food/fee 48 0 based on Regulation 2073 - Official sampling - Objective sampling d) Other processed food products and prepared dishes - meat based dishes - Retail - Bulgaria - single 25 Gram N_A 20 0 detection Listeria monocytogenes food sample - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 20 0 sampling d) Other processed food products and prepared dishes - meat based dishes - Retail - Bulgaria - single 25 Gram N_A 4 0 detection Listeria monocytogenes food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 4 0 d) Other processed food products and prepared dishes - meat based dishes - Retail - Not single 25 Gram N_A 12 0 detection Listeria monocytogenes Available - Not Available - Surveillance - HACCP and own check - Objective sampling (food/fee 12 0 d) Other processed food products and prepared dishes - pasta - filled pasta - Processing plant - batch 25 Gram N_A 1 1 detection Listeria monocytogenes O1/2 Bulgaria - food sample - Surveillance - based on Regulation 2073 - HACCP and own check - (food/fee 1 1 Objective sampling d) Other processed food products and prepared dishes - potato based dishes - Catering - Not single 1 Gram only enumeration 6 0 <= 100 Listeria monocytogenes 6 0 Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 6 0 Other processed food products and prepared dishes - potato based dishes - Catering - Not single 25 Gram only detection 4 0 detection Listeria monocytogenes Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee 4 0 d) Other processed food products and prepared dishes - Restaurant or Cafe or Pub or Bar or single 10 Gram N_A 46 0 <= 100 Listeria monocytogenes 46 0 Hotel or Catering service - Bulgaria - food sample - Surveillance - based on Regulation 2073 - (food/fee Official sampling - Objective sampling d) >100 Listeria monocytogenes 46 0 Other processed food products and prepared dishes - Restaurant or Cafe or Pub or Bar or single 1 Gram Only enumeration 20 0 <= 100 Listeria monocytogenes 20 0 Hotel or Catering service - Bulgaria - food sample - Surveillance - Official sampling - Objective (food/fee sampling d) >100 Listeria monocytogenes 20 0 Other processed food products and prepared dishes - Restaurant or Cafe or Pub or Bar or single 25 Gram Only detection 117 0 detection Listeria monocytogenes Hotel or Catering service - Bulgaria - food sample - Surveillance - Official sampling - Objective (food/fee 117 0 sampling d)

Bulgaria - 2019 36 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Other processed food products and prepared dishes - Retail - Bulgaria - food sample - single 25 Gram N_A 12 0 detection Listeria monocytogenes Surveillance - HACCP and own check - Objective sampling (food/fee 12 0 d) Other processed food products and prepared dishes - Retail - Bulgaria - food sample - single 1 Gram only enumeration 88 0 <= 100 Listeria monocytogenes 88 0 Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 88 0 Other processed food products and prepared dishes - Retail - Bulgaria - food sample - single 25 Gram only detection 106 0 detection Listeria monocytogenes Surveillance - Official sampling - Objective sampling (food/fee 106 0 d) Other processed food products and prepared dishes - sandwiches - Catering - Bulgaria - food single 25 Gram N_A 7 0 detection Listeria monocytogenes sample - Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 7 0 d) Other processed food products and prepared dishes - sandwiches - Processing plant - batch 25 Gram N_A 39 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - Official sampling - Objective sampling (food/fee 39 0 d) Other processed food products and prepared dishes - sandwiches - Processing plant - Not single 1 Gram only enumeration 8 0 <= 100 Listeria monocytogenes 8 0 Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 8 0 Other processed food products and prepared dishes - sandwiches - Processing plant - Not single 25 Gram only detection 2 0 detection Listeria monocytogenes Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee 2 0 d) Other processed food products and prepared dishes - sandwiches - Restaurant or Cafe or single 10 Gram N_A 12 0 <= 100 Listeria monocytogenes 12 0 Pub or Bar or Hotel or Catering service - Bulgaria - food sample - Surveillance - based on (food/fee Regulation 2073 - Official sampling - Objective sampling d) >100 Listeria monocytogenes 12 0 Other processed food products and prepared dishes - sandwiches - Restaurant or Cafe or single 25 Gram N_A 6 0 detection Listeria monocytogenes Pub or Bar or Hotel or Catering service - Bulgaria - food sample - Surveillance - HACCP and (food/fee 6 0 own check - Objective sampling d) Other processed food products and prepared dishes - sandwiches - Restaurant or Cafe or single 1 Gram Only enumeration 4 0 <= 100 Listeria monocytogenes 4 0 Pub or Bar or Hotel or Catering service - Bulgaria - food sample - Surveillance - Official (food/fee sampling - Objective sampling d) >100 Listeria monocytogenes 4 0 Other processed food products and prepared dishes - sandwiches - Restaurant or Cafe or single 25 Gram Only detection 7 0 detection Listeria monocytogenes Pub or Bar or Hotel or Catering service - Bulgaria - food sample - Surveillance - Official (food/fee 7 0 sampling - Objective sampling d) Other processed food products and prepared dishes - sandwiches - Retail - Bulgaria - food single 25 Gram N_A 11 0 detection Listeria monocytogenes sample - Surveillance - Official sampling - Objective sampling (food/fee 11 0 d) Other processed food products and prepared dishes - sandwiches - Retail - Not Available - single 1 Gram N_A 6 0 <= 100 Listeria monocytogenes 6 0 food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 6 0 Other processed food products and prepared dishes - sandwiches - with meat - Processing batch 25 Gram N_A 2 2 detection Listeria monocytogenes O1/2 plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - HACCP and own (food/fee 2 2 check - Objective sampling d) Other processed food products and prepared dishes - sandwiches - with meat - Processing batch 25 Gram N_A 1 1 detection Listeria monocytogenes O1/2 plant - Bulgaria - food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 1 1 d) Other processed food products and prepared dishes - sandwiches - with meat - Restaurant or single 25 Gram N_A 8 0 detection Listeria monocytogenes Cafe or Pub or Bar or Hotel or Catering service - Not Available - Not Available - Surveillance - (food/fee 8 0 based on Regulation 2073 - Official sampling - Objective sampling d) Other processed food products and prepared dishes - sandwiches - with meat - Retail - Not single 25 Gram N_A 4 0 detection Listeria monocytogenes Available - Not Available - Surveillance - HACCP and own check - Objective sampling (food/fee 4 0 d) Other processed food products and prepared dishes - unspecified - ready-to-eat foods - Retail single 1 Gram N_A 55 0 <= 100 Listeria monocytogenes 55 0 - Not Available - Not Available - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 55 0 Other processed food products and prepared dishes - unspecified - Restaurant or Cafe or batch 25 Gram N_A 3010 0 detection Listeria monocytogenes Pub or Bar or Hotel or Catering service - Not Available - Not Available - Surveillance - HACCP (food/fee 3,010 0 and own check - Objective sampling d) Other processed food products and prepared dishes - unspecified - Retail - Bulgaria - food single 25 Gram N_A 8 0 detection Listeria monocytogenes sample - Surveillance - HACCP and own check - Objective sampling (food/fee 8 0 d) Other processed food products and prepared dishes - unspecified - Retail - Bulgaria - food single 25 Gram N_A 115 0 detection Listeria monocytogenes sample - Surveillance - Official sampling - Objective sampling (food/fee 115 0 d) Other processed food products and prepared dishes - vegetable based dishes - Catering - single 1 Gram only enumeration 5 0 <= 100 Listeria monocytogenes 5 0 Not Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0

Bulgaria - 2019 37 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Other processed food products and prepared dishes - vegetable based dishes - Catering - single 25 Gram only detection 22 0 detection Listeria monocytogenes Not Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee 22 0 d) Other processed food products and prepared dishes - vegetable based dishes - Restaurant or single 1 Gram N_A 1 0 <= 100 Listeria monocytogenes 1 0 Cafe or Pub or Bar or Hotel or Catering service - Not Available - food sample - Surveillance - (food/fee Official sampling - Objective sampling d) >100 Listeria monocytogenes 1 0 Other processed food products and prepared dishes - vegetable based dishes - Retail - batch 25 Gram N_A 12 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - Official sampling - Objective sampling (food/fee 12 0 d) Other products of animal origin - Processing plant - Not Available - food sample - Surveillance single 25 Gram N_A 10 0 detection Listeria monocytogenes - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Ready-to-eat salads - Processing plant - Bulgaria - food sample - Surveillance - based on single 10 Gram N_A 2 0 <= 100 Listeria monocytogenes 2 0 Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 2 0 Ready-to-eat salads - Processing plant - Bulgaria - food sample - Surveillance - Official batch 25 Gram N_A 15 0 detection Listeria monocytogenes sampling - Objective sampling (food/fee 15 0 d) Ready-to-eat salads - Processing plant - Not Available - food sample - Surveillance - Official single 1 Gram only enumeration 20 0 <= 100 Listeria monocytogenes 20 0 sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 20 0 Ready-to-eat salads - Processing plant - Not Available - food sample - Surveillance - Official single 25 Gram only detection 55 0 detection Listeria monocytogenes sampling - Objective sampling (food/fee 55 0 d) Sauce and dressings - mayonnaise - Processing plant - Bulgaria - food sample - Surveillance single 25 Gram N_A 45 0 detection Listeria monocytogenes - HACCP and own check - Objective sampling (food/fee 45 0 d) Sauce and dressings - Processing plant - Not Available - food sample - Surveillance - based single 25 Gram N_A 10 0 detection Listeria monocytogenes on Regulation 2073 - Official sampling - Objective sampling (food/fee 10 0 d) Seeds, sprouted - ready-to-eat - Processing plant - Bulgaria - food sample - Surveillance - single 25 Gram N_A 1 0 detection Listeria monocytogenes 1 0 based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) 10 0 detection Listeria monocytogenes 10 0 Seeds, sprouted - ready-to-eat - Processing plant - Bulgaria - food sample - Surveillance - batch 25 Gram N_A 5 0 detection Listeria monocytogenes HACCP and own check - Objective sampling (food/fee 5 0 d) Seeds, sprouted - ready-to-eat - Restaurant or Cafe or Pub or Bar or Hotel or Catering service single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 - Bulgaria - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Seeds, sprouted - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - based on single 10 Gram N_A 1 0 <= 100 Listeria monocytogenes 1 0 Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 1 0 Seeds, sprouted - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - based on single 25 Gram N_A 25 0 detection Listeria monocytogenes Regulation 2073 - Official sampling - Objective sampling (food/fee 25 0 d) Seeds, sprouted - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - Official single 25 Gram N_A 5 0 detection Listeria monocytogenes sampling - Objective sampling (food/fee 5 0 d) Seeds, sprouted - ready-to-eat - Retail - Not Available - food sample - Surveillance - Official single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Seeds, sprouted - ready-to-eat - Retail - Not Available - Not Available - Surveillance - based single 25 Gram N_A 2 0 detection Listeria monocytogenes on Regulation 2073 - Official sampling - Objective sampling (food/fee 2 0 d) Snails - Processing plant - Bulgaria - food sample - Surveillance - HACCP and own check - batch 25 Gram N_A 1 0 detection Listeria monocytogenes Objective sampling (food/fee 1 0 d) Soups - ready-to-eat - Catering - Bulgaria - food sample - Surveillance - based on Regulation single 25 Gram N_A 3 0 detection Listeria monocytogenes 3 0 2073 - Official sampling - Objective sampling (food/fee d) 44 0 detection Listeria monocytogenes 44 0 Soups - ready-to-eat - Catering - Bulgaria - food sample - Surveillance - based on Regulation single 25 Gram N_A 1 0 detection Listeria monocytogenes 2073 - Official sampling - Suspect sampling (food/fee 1 0 d) Soups - ready-to-eat - Catering - Not Available - food sample - Surveillance - Official sampling single 1 Gram only enumeration 2 0 <= 100 Listeria monocytogenes 2 0 - Objective sampling (food/fee d) >100 Listeria monocytogenes 2 0

Bulgaria - 2019 38 Sample Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sample weight units units Metrics N of units N of units Area of Sampling Sampling strategy unit weight unit Sampling Details tested positive Method Zoonoses tested positive Not Available Soups - ready-to-eat - Catering - Not Available - food sample - Surveillance - Official sampling single 25 Gram only detection 31 0 detection Listeria monocytogenes - Objective sampling (food/fee 31 0 d) Soups - ready-to-eat - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - single 10 Gram N_A 27 0 <= 100 Listeria monocytogenes 27 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee Objective sampling d) >100 Listeria monocytogenes 27 0 Soups - ready-to-eat - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - single 25 Gram N_A 7 0 detection Listeria monocytogenes Bulgaria - food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 7 0 d) Soups - ready-to-eat - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - Not single 1 Gram N_A 2 0 <= 100 Listeria monocytogenes 2 0 Available - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 2 0 Soups - ready-to-eat - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - Not single 25 Gram N_A 25 0 detection Listeria monocytogenes Available - Not Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 25 0 Objective sampling d) Soups - ready-to-eat - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - Not single 1 Gram N_A 98 0 <= 100 Listeria monocytogenes 98 0 Available - Not Available - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 98 0 Soups - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - HACCP and own check batch 25 Gram N_A 2 0 detection Listeria monocytogenes - Objective sampling (food/fee 2 0 d) Soups - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - Official sampling - batch 25 Gram N_A 19 0 detection Listeria monocytogenes Objective sampling (food/fee 19 0 d) Vegetables - non-pre-cut - Processing plant - Not Available - Not Available - Surveillance - batch 25 Gram N_A 25 0 detection Listeria monocytogenes HACCP and own check - Objective sampling (food/fee 25 0 d) Vegetables - pre-cut - ready-to-eat - Processing plant - Bulgaria - food sample - Surveillance - batch 25 Gram N_A 40 0 detection Listeria monocytogenes Official sampling - Objective sampling (food/fee 40 0 d) Vegetables - pre-cut - ready-to-eat - Processing plant - Not Available - food sample - single 1 Gram N_A 5 0 <= 100 Listeria monocytogenes 5 0 Surveillance - based on Regulation 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 5 0 Vegetables - pre-cut - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - based on single 25 Gram N_A 90 0 detection Listeria monocytogenes Regulation 2073 - Official sampling - Objective sampling (food/fee 90 0 d) Vegetables - pre-cut - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - HACCP single 1 Gram N_A 10 0 detection Listeria monocytogenes and own check - Objective sampling (food/fee 10 0 d) Vegetables - pre-cut - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - Official single 25 Gram N_A 60 0 detection Listeria monocytogenes sampling - Objective sampling (food/fee 60 0 d) Vegetables - pre-cut - ready-to-eat - Retail - Not Available - food sample - Surveillance - single 1 Gram N_A 28 0 <= 100 Listeria monocytogenes 28 0 Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 28 0 Vegetables - pre-cut - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - Bulgaria single 1 Gram N_A 55 0 <= 100 Listeria monocytogenes 55 0 - food sample - Surveillance - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 55 0 Vegetables - pre-cut - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - Not single 25 Gram N_A 16 0 detection Listeria monocytogenes Available - Not Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 16 0 Objective sampling d) Vegetables - pre-cut - Retail - Bulgaria - food sample - Surveillance - based on Regulation single 10 Gram N_A 12 0 <= 100 Listeria monocytogenes 12 0 2073 - Official sampling - Objective sampling (food/fee d) >100 Listeria monocytogenes 12 0 Vegetables - products - Processing plant - Bulgaria - food sample - Surveillance - HACCP and single 25 Gram N_A 120 0 detection Listeria monocytogenes own check - Objective sampling (food/fee 120 0 d) Water - bottled water - Processing plant - Bulgaria - food sample - Surveillance - Official single 1 Millilitre N_A 2 0 detection Listeria monocytogenes sampling - Objective sampling (food/fee 2 0 d)

Bulgaria - 2019 39 Table Lyssavirus:LYSSAVIRUS in animal

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive BULGARIA Cats - pet animals - Veterinary clinics - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 3 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Cats - stray cats - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 3 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Deer - wild - roe deer - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Dogs - pet animals - Veterinary clinics - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 5 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Dogs - stray dogs - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 2 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 805 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 90 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 1042 0 European Bat Lyssavirus - 0 sampling unspecified Jackals - wild - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 35 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Sheep - Farm - Not Available - Not Available - Monitoring - passive - Official sampling - Suspect sampling N_A Not Available animal 2 0 European Bat Lyssavirus - 0 unspecified Wolves - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 4 0 European Bat Lyssavirus - 0 sampling unspecified Видин Cats - pet animals - Veterinary clinics - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 23 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 3 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 69 0 European Bat Lyssavirus - 0 sampling unspecified Jackals - wild - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 2 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Монтана Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 10 0 European Bat Lyssavirus - 0 Objective sampling unspecified Враца Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 73 0 European Bat Lyssavirus - 0 Objective sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 110 0 European Bat Lyssavirus - 0 sampling unspecified Плевен Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 32 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 3 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 56 0 European Bat Lyssavirus - 0 sampling unspecified Jackals - wild - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 3 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Ловеч Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 7 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 12 0 European Bat Lyssavirus - 0 sampling unspecified Велико Търново Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 5 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 3 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 18 0 European Bat Lyssavirus - 0 sampling unspecified Габрово Cats - stray cats - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 3 0 European Bat Lyssavirus - 0 Suspect sampling unspecified

Bulgaria - 2019 40 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Габрово Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 112 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 8 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 122 0 European Bat Lyssavirus - 0 sampling unspecified Jackals - wild - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Wolves - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 1 0 European Bat Lyssavirus - 0 sampling unspecified Русе Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 3 0 European Bat Lyssavirus - 0 Objective sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 5 0 European Bat Lyssavirus - 0 sampling unspecified Разград Cats - pet animals - Veterinary clinics - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Dogs - pet animals - Veterinary clinics - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 151 0 European Bat Lyssavirus - 0 Objective sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 167 0 European Bat Lyssavirus - 0 sampling unspecified Силистра Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 87 0 European Bat Lyssavirus - 0 Objective sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 73 0 European Bat Lyssavirus - 0 sampling unspecified Варна Dogs - pet animals - Veterinary clinics - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Добрич Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 11 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 1 0 European Bat Lyssavirus - 0 sampling unspecified Шумен Dogs - pet animals - Veterinary clinics - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Dogs - stray dogs - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 5 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 4 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 2 0 European Bat Lyssavirus - 0 sampling unspecified Бургас Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 9 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 2 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 55 0 European Bat Lyssavirus - 0 sampling unspecified Jackals - wild - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 7 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Sheep - Farm - Not Available - Not Available - Monitoring - passive - Official sampling - Suspect sampling N_A Not Available animal 2 0 European Bat Lyssavirus - 0 unspecified Сливен Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 1 0 European Bat Lyssavirus - 0 Objective sampling unspecified Wolves - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 1 0 European Bat Lyssavirus - 0 sampling unspecified Ямбол Cats - pet animals - Veterinary clinics - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 9 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified

Bulgaria - 2019 41 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Ямбол Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 27 0 European Bat Lyssavirus - 0 sampling unspecified Jackals - wild - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 5 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Стара Загора Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 4 0 European Bat Lyssavirus - 0 sampling unspecified София (столица) Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 10 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 2 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 1 0 European Bat Lyssavirus - 0 sampling unspecified София Deer - wild - roe deer - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Dogs - pet animals - Veterinary clinics - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 44 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 32 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 13 0 European Bat Lyssavirus - 0 sampling unspecified Jackals - wild - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 3 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Благоевград Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 37 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 10 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Перник Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 11 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 2 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 2 0 European Bat Lyssavirus - 0 sampling unspecified Jackals - wild - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Кюстендил Dogs - pet animals - Veterinary clinics - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Dogs - stray dogs - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 1 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 8 0 European Bat Lyssavirus - 0 Objective sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 8 0 European Bat Lyssavirus - 0 sampling unspecified Пловдив Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 1 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 1 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 3 0 European Bat Lyssavirus - 0 sampling unspecified Хасково Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 28 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 2 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 220 0 European Bat Lyssavirus - 0 sampling unspecified Jackals - wild - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 9 0 European Bat Lyssavirus - 0 Suspect sampling unspecified Wolves - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 2 0 European Bat Lyssavirus - 0 sampling unspecified Пазарджик Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 12 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 2 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified

Bulgaria - 2019 42 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Пазарджик Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 11 0 European Bat Lyssavirus - 0 sampling unspecified Смолян Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 13 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 3 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Кърджали Foxes - wild - red fox - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - N_A Not Available animal 103 0 European Bat Lyssavirus - 0 Objective sampling unspecified Foxes - wild - red fox - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling N_A Not Available animal 10 0 European Bat Lyssavirus - 0 - Suspect sampling unspecified Jackals - wild - Hunting - Not Available - Not Available - Monitoring - active - Official sampling - Objective N_A Not Available animal 63 0 European Bat Lyssavirus - 0 sampling unspecified Jackals - wild - Natural habitat - Not Available - Not Available - Monitoring - passive - Official sampling - N_A Not Available animal 4 0 European Bat Lyssavirus - 0 Suspect sampling unspecified

Bulgaria - 2019 43 Table Marine biotoxins:MARINE BIOTOXINS in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Molluscan shellfish - raw - Farm - Bulgaria - food sample - Surveillance - batch 1 Gram N_A Not Available 17 0 Marine biotoxins 0 HACCP and own check - Objective sampling (food/fee d) Molluscan shellfish - raw - Farm - Bulgaria - food sample - Surveillance - single 1 Gram N_A Not Available 18 0 Marine biotoxins 0 Official sampling - Objective sampling (food/fee d) Molluscan shellfish - raw - frozen - Border Control Posts - Turkey - food single 1 Gram N_A Not Available 33 0 Marine biotoxins 0 sample - Surveillance - Official sampling - Objective sampling (food/fee d) Molluscan shellfish - raw - Packing centre - Bulgaria - food sample - single 1 Gram N_A Not Available 14 0 Marine biotoxins 0 Surveillance - Official sampling - Objective sampling (food/fee d) Molluscan shellfish - raw - Retail - Bulgaria - food sample - Surveillance - single 1 Gram N_A Not Available 12 0 Marine biotoxins 0 Official sampling - Objective sampling (food/fee d)

Bulgaria - 2019 44 Table Salmonella:SALMONELLA in animal

N of flocks Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling under control Target Total units units Metrics N of units Area of Sampling strategy unit programme verification Sampling Details Method tested positive Zoonoses positive Not Available Gallus gallus (fowl) - breeding flocks, unspecified - adult - Farm - Bulgaria - animal sample - faeces - Control herd/floc 263 Y N_A ISO 6579- 199 4 Salmonella Enteritidis 1 and eradication programmes - Official and industry sampling - Census k 1:2017 Salmonella Infantis 2 Salmonella Salmonella Typhimurium 1 Gallus gallus (fowl) - broilers - before slaughter - Farm - Bulgaria - animal sample - faeces - Control and herd/floc 5790 N_A N_A ISO 6579- 5790 0 Salmonella eradication programmes - Industry sampling - Census k 1:2017 0 Salmonella Gallus gallus (fowl) - broilers - before slaughter - Farm - Bulgaria - animal sample - faeces - Control and herd/floc 230 Y N_A ISO 6579- 230 2 Salmonella Typhimurium eradication programmes - Official and industry sampling - Census k 1:2017 2 Salmonella Gallus gallus (fowl) - broilers - before slaughter - Farm - Bulgaria - animal sample - faeces - Control and herd/floc 230 N_A N_A ISO 6579- 230 2 Salmonella Typhimurium eradication programmes - Official sampling - Objective sampling k 1:2017 2 Salmonella Gallus gallus (fowl) - laying hens - adult - Farm - Bulgaria - animal sample - faeces - Control and eradication herd/floc 211 Y N_A ISO 6579- 211 12 Salmonella Agona 1 programmes - Official and industry sampling - Census k 1:2017 Salmonella Enteritidis 7 Salmonella Salmonella Infantis 3 Salmonella Senftenberg 1 Gallus gallus (fowl) - laying hens - during rearing period - Farm - Bulgaria - animal sample - faeces - Control herd/floc 94 N_A N_A ISO 6579- 94 2 Salmonella Enteritidis and eradication programmes - Official and industry sampling - Census k 1:2017 2 Salmonella Turkeys - breeding flocks, unspecified - adult - Farm - Bulgaria - animal sample - faeces - Control and herd/floc 3 N_A N_A ISO 6579- 3 0 Salmonella eradication programmes - Industry sampling - Census k 1:2017 0 Salmonella Turkeys - breeding flocks, unspecified - adult - Farm - Bulgaria - animal sample - faeces - Control and herd/floc 3 Y N_A ISO 6579- 3 0 Salmonella eradication programmes - Official and industry sampling - Census k 1:2017 0 Salmonella Turkeys - breeding flocks, unspecified - adult - Farm - Bulgaria - animal sample - faeces - Control and herd/floc 3 N_A N_A ISO 6579- 3 0 Salmonella eradication programmes - Official sampling - Objective sampling k 1:2017 0 Salmonella Turkeys - fattening flocks - before slaughter - Farm - Bulgaria - animal sample - faeces - Control and herd/floc 4 N_A N_A ISO 6579- 4 0 Salmonella eradication programmes - Industry sampling - Census k 1:2017 0 Salmonella Turkeys - fattening flocks - before slaughter - Farm - Bulgaria - animal sample - faeces - Control and herd/floc 4 Y N_A ISO 6579- 4 0 Salmonella eradication programmes - Official and industry sampling - Census k 1:2017 0 Salmonella Turkeys - fattening flocks - before slaughter - Farm - Bulgaria - animal sample - faeces - Control and herd/floc 4 N_A N_A ISO 6579- 2 0 Salmonella eradication programmes - Official sampling - Objective sampling k 1:2017 0 Salmonella

Bulgaria - 2019 45 Table Salmonella:SALMONELLA in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Bakery products - bread - Processing plant - Not Available - food sample - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Bakery products - cakes - containing heat-treated cream - Processing single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 plant - Not Available - Not Available - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Bakery products - cakes - containing heat-treated cream - Retail - Not single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Available - Not Available - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Bakery products - cakes - containing raw cream - Processing plant - Not single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Bakery products - cakes - Processing plant - Bulgaria - food sample - single 25 Gram N_A Not Available 29 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee d) Bakery products - cakes - Restaurant or Cafe or Pub or Bar or Hotel or single 25 Gram N_A ISO 6579- 65 0 Salmonella 0 Catering service - Bulgaria - food sample - Surveillance - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Bakery products - desserts - containing heat-treated cream - Processing single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 plant - Not Available - Not Available - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Bakery products - desserts - containing heat-treated cream - Retail - Not single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Available - Not Available - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Bakery products - desserts - containing raw cream - Processing plant - Not single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Bakery products - desserts - containing raw eggs and cream - Processing batch 25 Gram N_A ISO 6579- 2 0 Salmonella 0 plant - Bulgaria - food sample - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Bakery products - desserts - containing raw eggs and cream - Processing single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 plant - Not Available - Not Available - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Bakery products - desserts - containing raw eggs and cream - Retail - Not single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Available - Not Available - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Bakery products - desserts - Restaurant or Cafe or Pub or Bar or Hotel or single 25 Gram N_A ISO 6579- 70 0 Salmonella 0 Catering service - Bulgaria - food sample - Surveillance - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Bakery products - pastry - Processing plant - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Bakery products - pastry - with egg filling - Processing plant - Not single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Bakery products - Processing plant - Bulgaria - food sample - Surveillance single 25 Gram N_A ISO 6579- 16 0 Salmonella 0 - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Bakery products - Retail - Bulgaria - food sample - Surveillance - HACCP single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 and own check - Objective sampling (food/fee 1:2017 d) Salmonella Bakery products - Retail - Bulgaria - food sample - Surveillance - Official single 25 Gram N_A ISO 6579- 11 0 Salmonella 0 sampling - Objective sampling (food/fee 1:2017 d) Salmonella Beverages, alcoholic - Processing plant - Bulgaria - food sample - batch 25 Gram N_A Slide 1 1 Salmonella Kottbus 1 Surveillance - HACCP and own check - Objective sampling (food/fee agglutination d) according White Kauffmann Le Minor Scheme

Bulgaria - 2019 46 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Beverages, non-alcoholic - soft drinks - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 sample - meat - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Beverages, non-alcoholic - soft drinks - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 sample - meat - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Cereals and meals - flour/meal or finely ground powder - Processing plant single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 - Not Available - food sample - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Cereals and meals - Restaurant or Cafe or Pub or Bar or Hotel or Catering single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 service - Not Available - Not Available - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Cheeses made from cows' milk - curd - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from cows' milk - curd - Processing plant - Not Available - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 food sample - Surveillance - based on Regulation 2073 - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Cheeses made from cows' milk - curd - Processing plant - Not Available - single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 Not Available - Surveillance - based on Regulation 2073 - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Cheeses made from cows' milk - curd - Processing plant - Not Available - single 25 Gram N_A ISO 6579- 12 0 Salmonella 0 Not Available - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from cows' milk - curd - Retail - Not Available - food single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from cows' milk - fresh - made from pasteurised milk - single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Cheeses made from cows' milk - fresh - Processing plant - Not Available - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Not Available - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from cows' milk - hard - made from pasteurised milk - single 25 Gram N_A ISO 6579- 125 0 Salmonella 0 Processing plant - Bulgaria - food sample - meat - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Cheeses made from cows' milk - hard - made from pasteurised milk - single 25 Gram N_A ISO 6579- 40 0 Salmonella 0 Processing plant - Bulgaria - food sample - milk - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Cheeses made from cows' milk - hard - made from pasteurised milk - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Cheeses made from cows' milk - hard - made from pasteurised milk - single 25 Gram N_A ISO 6579- 60 0 Salmonella 0 Processing plant - Not Available - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Cheeses made from cows' milk - hard - made from pasteurised milk - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Retail - Bulgaria - food sample - milk - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Cheeses made from cows' milk - hard - made from pasteurised milk - single 25 Gram N_A ISO 6579- 50 0 Salmonella 0 Retail - Not Available - food sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Cheeses made from cows' milk - hard - made from raw or low heat- single 25 Gram N_A ISO 6579- 12 0 Salmonella 0 treated milk - Processing plant - Bulgaria - food sample - Surveillance - (food/fee 1:2017 based on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Cheeses made from cows' milk - hard - made from raw or low heat- single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 treated milk - Retail - Bulgaria - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Cheeses made from cows' milk - hard - Processing plant - Bulgaria - food single 25 Gram N_A Not Available 17 0 Salmonella 0 sample - milk - Surveillance - HACCP and own check - Objective sampling (food/fee d) Cheeses made from cows' milk - hard - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 90 0 Salmonella 0 sample - milk - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella

Bulgaria - 2019 47 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Cheeses made from cows' milk - hard - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 85 0 Salmonella 0 sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from cows' milk - hard - Processing plant - Not Available - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Not Available - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from cows' milk - hard - Processing plant - Not Available - single 25 Gram N_A ISO 6579- 25 0 Salmonella 0 Not Available - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from cows' milk - Processing plant - Bulgaria - food sample single 25 Gram N_A Not Available 8 0 Salmonella 0 - milk - Surveillance - HACCP and own check - Objective sampling (food/fee d) Cheeses made from cows' milk - soft and semi-soft - made from single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 pasteurised milk - Border Control Posts - Serbia - food sample - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Cheeses made from cows' milk - soft and semi-soft - made from single 25 Gram N_A ISO 6579- 35 0 Salmonella 0 pasteurised milk - Processing plant - Bulgaria - food sample - milk - (food/fee 1:2017 Surveillance - HACCP and own check - Objective sampling d) Salmonella Cheeses made from cows' milk - soft and semi-soft - made from single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 pasteurised milk - Processing plant - Bulgaria - food sample - milk - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Cheeses made from cows' milk - soft and semi-soft - made from single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 pasteurised milk - Processing plant - Bulgaria - food sample - Surveillance (food/fee 1:2017 - based on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Cheeses made from cows' milk - soft and semi-soft - made from single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 pasteurised milk - Processing plant - Not Available - Not Available - (food/fee 1:2017 Surveillance - based on Regulation 2073 - Official sampling - Objective d) Salmonella sampling Cheeses made from cows' milk - soft and semi-soft - made from single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 pasteurised milk - Retail - Bulgaria - food sample - milk - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Cheeses made from cows' milk - soft and semi-soft - made from single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 pasteurised milk - Retail - Bulgaria - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Cheeses made from cows' milk - soft and semi-soft - made from single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 pasteurised milk - Retail - Not Available - food sample - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Cheeses made from cows' milk - soft and semi-soft - Processing plant - single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Not Available - Not Available - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Cheeses made from cows' milk - soft and semi-soft - Processing plant - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Not Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Cheeses made from cows' milk - unspecified - made from pasteurised milk batch 200 Gram N_A Not Available 94 0 Salmonella 0 - Game handling establishment - Not Available - Not Available - (food/fee Surveillance - HACCP and own check - Objective sampling d) Cheeses made from goats' milk - hard - made from pasteurised milk - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Processing plant - Not Available - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Cheeses made from goats' milk - hard - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 55 0 Salmonella 0 sample - milk - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from goats' milk - hard - Processing plant - Not Available - single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 Not Available - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from goats' milk - hard - Processing plant - Not Available - single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 Not Available - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from goats' milk - Processing plant - Bulgaria - food sample single 25 Gram N_A Not Available 5 0 Salmonella 0 - milk - Surveillance - HACCP and own check - Objective sampling (food/fee d) Cheeses made from goats' milk - soft and semi-soft - made from single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 pasteurised milk - Processing plant - Bulgaria - food sample - milk - (food/fee 1:2017 Surveillance - HACCP and own check - Objective sampling d) Salmonella

Bulgaria - 2019 48 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Cheeses made from goats' milk - soft and semi-soft - Processing plant - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Not Available - Not Available - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Cheeses made from goats' milk - soft and semi-soft - Processing plant - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Not Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Cheeses made from sheep's milk - hard - made from pasteurised milk - single 25 Gram N_A ISO 6579- 85 0 Salmonella 0 Processing plant - Bulgaria - food sample - milk - Surveillance - HACCP (food/fee 1:2017 and own check - Objective sampling d) Salmonella Cheeses made from sheep's milk - hard - made from pasteurised milk - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Processing plant - Not Available - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Cheeses made from sheep's milk - hard - made from pasteurised milk - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Retail - Not Available - food sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Cheeses made from sheep's milk - hard - Processing plant - Bulgaria - single 25 Gram N_A ISO 6579- 120 0 Salmonella 0 food sample - milk - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Cheeses made from sheep's milk - hard - Processing plant - Not Available single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 - Not Available - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from sheep's milk - hard - Processing plant - Not Available single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 - Not Available - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Cheeses made from sheep's milk - soft and semi-soft - made from single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 pasteurised milk - Processing plant - Bulgaria - food sample - milk - (food/fee 1:2017 Surveillance - HACCP and own check - Objective sampling d) Salmonella Cheeses made from sheep's milk - soft and semi-soft - made from single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 pasteurised milk - Retail - Bulgaria - food sample - milk - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Cheeses made from sheep's milk - soft and semi-soft - Processing plant - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Not Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Cheeses, made from mixed milk from cows, sheep and/or goats - hard - single 25 Gram N_A ISO 6579- 35 0 Salmonella 0 Processing plant - Bulgaria - food sample - milk - Surveillance - HACCP (food/fee 1:2017 and own check - Objective sampling d) Salmonella Cheeses, made from mixed milk from cows, sheep and/or goats - hard - single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 Processing plant - Bulgaria - food sample - milk - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Cheeses, made from mixed milk from cows, sheep and/or goats - hard - single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 Processing plant - Not Available - Not Available - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Cheeses, made from mixed milk from cows, sheep and/or goats - single 25 Gram N_A Not Available 3 0 Salmonella 0 Processing plant - Bulgaria - food sample - milk - Surveillance - HACCP (food/fee and own check - Objective sampling d) Cheeses, made from mixed milk from cows, sheep and/or goats - soft and single 25 Gram N_A ISO 6579- 50 0 Salmonella 0 semi-soft - made from pasteurised milk - Processing plant - Bulgaria - (food/fee 1:2017 food sample - milk - Surveillance - HACCP and own check - Objective d) Salmonella sampling Cheeses, made from mixed milk from cows, sheep and/or goats - soft and single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 semi-soft - made from pasteurised milk - Processing plant - Bulgaria - (food/fee 1:2017 food sample - milk - Surveillance - Official sampling - Objective sampling d) Salmonella Cheeses, made from mixed milk from cows, sheep and/or goats - soft and single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 semi-soft - Processing plant - Not Available - Not Available - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Cheeses, made from mixed milk from cows, sheep and/or goats - batch 25 Gram N_A Not Available 2204 0 Salmonella 0 unspecified - Processing plant - Not Available - Not Available - Surveillance (food/fee - HACCP and own check - Objective sampling d) Cheeses, made from unspecified milk or other animal milk - fresh - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Processing plant - Not Available - Not Available - Surveillance - based on (food/fee 1:2017 Regulation 2073 - HACCP and own check - Objective sampling d) Salmonella

Bulgaria - 2019 49 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Cheeses, made from unspecified milk or other animal milk - Retail - Not single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Available - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Cheeses, made from unspecified milk or other animal milk - soft and semi- single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 soft - Retail - Bulgaria - food sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Cocoa and cocoa preparations, coffee and tea - Retail - Bulgaria - food single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 sample - meat - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Confectionery products and pastes - chocolate-based product - Processing single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 plant - Bulgaria - food sample - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Confectionery products and pastes - chocolate-based product - Processing single 25 Gram N_A ISO 6579- 23 0 Salmonella 0 plant - Bulgaria - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Confectionery products and pastes - chocolate-based product - Processing single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 plant - Not Available - Not Available - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Confectionery products and pastes - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 sample - meat - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Confectionery products and pastes - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 8 0 Salmonella 0 sample - meat - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Confectionery products and pastes - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 43 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Confectionery products and pastes - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 12 0 Salmonella 0 sample - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Confectionery products and pastes - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 33 0 Salmonella 0 sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Confectionery products and pastes - Processing plant - Not Available - food single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 sample - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Confectionery products and pastes - Processing plant - Not Available - food single 25 Gram N_A ISO 6579- 27 0 Salmonella 0 sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Confectionery products and pastes - Retail - Bulgaria - food sample - meat single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Confectionery products and pastes - Retail - Bulgaria - food sample - meat single 25 Gram N_A ISO 6579- 22 0 Salmonella 0 - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Confectionery products and pastes - Retail - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 11 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Confectionery products and pastes - Retail - Not Available - food sample - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Dairy products (excluding cheeses) - butter - made from pasteurised milk single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 - Processing plant - Bulgaria - food sample - milk - Surveillance - HACCP (food/fee 1:2017 and own check - Objective sampling d) Salmonella Dairy products (excluding cheeses) - butter - made from pasteurised milk single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 - Processing plant - Bulgaria - food sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - butter - made from pasteurised milk single 25 Gram N_A ISO 6579- 30 0 Salmonella 0 - Processing plant - Not Available - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - butter - made from pasteurised milk single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 - Processing plant - Not Available - Not Available - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella

Bulgaria - 2019 50 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Dairy products (excluding cheeses) - butter - made from pasteurised milk single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 - Retail - Bulgaria - food sample - milk - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Dairy products (excluding cheeses) - butter - made from pasteurised milk single 25 Gram N_A ISO 6579- 30 0 Salmonella 0 - Retail - Bulgaria - food sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Dairy products (excluding cheeses) - butter - Processing plant - Bulgaria - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Dairy products (excluding cheeses) - butter - Processing plant - Bulgaria - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 food sample - Surveillance - based on Regulation 2073 - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Dairy products (excluding cheeses) - butter - Processing plant - Not single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Dairy products (excluding cheeses) - butter - Retail - Bulgaria - food single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Dairy products (excluding cheeses) - cheese analogue - Processing plant - single 25 Gram N_A ISO 6579- 25 0 Salmonella 0 Bulgaria - food sample - meat - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Dairy products (excluding cheeses) - cheese analogue - Processing plant - single 25 Gram N_A ISO 6579- 35 0 Salmonella 0 Bulgaria - food sample - milk - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Dairy products (excluding cheeses) - cheese analogue - Processing plant - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - cheese analogue - Processing plant - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Not Available - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 HACCP and own check - Objective sampling d) Salmonella Dairy products (excluding cheeses) - cheese analogue - Processing plant - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Not Available - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - cheese analogue - Retail - Not single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 Available - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Dairy products (excluding cheeses) - dairy desserts - Processing plant - single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Not Available - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - dairy products, not specified - made single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 from raw or low heat-treated milk - Processing plant - Not Available - Not (food/fee 1:2017 Available - Surveillance - Official sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - ice-cream - made from pasteurised single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 milk - Processing plant - Bulgaria - food sample - milk - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - ice-cream - made from pasteurised single 25 Gram N_A ISO 6579- 300 0 Salmonella 0 milk - Processing plant - Bulgaria - food sample - Surveillance - HACCP (food/fee 1:2017 and own check - Objective sampling d) Salmonella Dairy products (excluding cheeses) - ice-cream - made from pasteurised single 25 Gram N_A ISO 6579- 45 0 Salmonella 0 milk - Processing plant - Bulgaria - food sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - ice-cream - made from pasteurised single 25 Millilitre N_A ISO 6579- 30 0 Salmonella 0 milk - Processing plant - Not Available - food sample - Surveillance - based (food/fee 1:2017 on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - ice-cream - Processing plant - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - ice-cream - Processing plant - Not single 25 Gram N_A ISO 6579- 12 0 Salmonella 0 Available - Not Available - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Dairy products (excluding cheeses) - ice-cream - Processing plant - Not single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Bulgaria - 2019 51 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Dairy products (excluding cheeses) - milk powder and whey powder - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Packing centre - Bulgaria - food sample - Surveillance - HACCP and own (food/fee 1:2017 check - Objective sampling d) Salmonella Dairy products (excluding cheeses) - sour milk - Processing plant - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - sour milk - Processing plant - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Bulgaria - food sample - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Dairy products (excluding cheeses) - sour milk - Processing plant - Not single 25 Millilitre N_A ISO 6579- 5 0 Salmonella 0 Available - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Dairy products (excluding cheeses) - sour milk - Retail - Bulgaria - food single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Dairy products (excluding cheeses) - sour milk - Retail - Not Available - single 25 Millilitre N_A ISO 6579- 10 0 Salmonella 0 food sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Dairy products (excluding cheeses) - yoghurt - Processing plant - Bulgaria single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 - food sample - meat - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Dairy products (excluding cheeses) - yoghurt - Processing plant - Bulgaria single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 - food sample - milk - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Dairy products (excluding cheeses) - yoghurt - Processing plant - Not single 25 Gram N_A ISO 6579- 22 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Dairy products, unspecified - Processing plant - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Egg products - dried - Processing plant - Not Available - Not Available - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Egg products - liquid - Processing plant - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Egg products - Processing plant - Not Available - Not Available - batch 25 Gram N_A Not Available 309 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee d) Eggs - Farm - Not Available - Not Available - Surveillance - HACCP and batch 25 Gram N_A Not Available 982 0 Salmonella 0 own check - Objective sampling (food/fee d) Eggs - Processing plant - Not Available - Not Available - Surveillance - single 25 Gram N_A ISO 6579- 7 0 Salmonella 0 HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Eggs - Processing plant - Not Available - Not Available - Surveillance - single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Eggs - quail eggs - Processing plant - Bulgaria - food sample - Surveillance single 25 Gram N_A Not Available 12 0 Salmonella 0 - HACCP and own check - Objective sampling (food/fee d) Eggs - Retail - Bulgaria - food sample - meat - Surveillance - HACCP and single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 own check - Objective sampling (food/fee 1:2017 d) Salmonella Eggs - table eggs - Farm - Not Available - Not Available - Surveillance - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 based on Regulation 2073 - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Eggs - table eggs - Packing centre - Bulgaria - food sample - Surveillance - single 25 Gram N_A ISO 6579- 26 0 Salmonella 0 HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella 170 0 Salmonella 0 Eggs - table eggs - Packing centre - Bulgaria - food sample - Surveillance - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella 30 0 Salmonella 0 Eggs - table eggs - Packing centre - Not Available - food sample - single 25 Millilitre N_A ISO 6579- 15 0 Salmonella 0 Surveillance - based on Regulation 2073 - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Bulgaria - 2019 52 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Eggs - table eggs - Packing centre - Not Available - Not Available - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Eggs - table eggs - Packing centre - Not Available - Not Available - single 25 Gram N_A Not Available 136 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee d) Eggs - table eggs - shell - Wholesale - Poland - food sample - Surveillance single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Eggs - table eggs - white - Wholesale - Poland - food sample - Surveillance single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Eggs - table eggs - yolk - Wholesale - Poland - food sample - Surveillance single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 - based on Regulation 2073 - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Fish - raw - frozen - Processing plant - Bulgaria - food sample - single 25 Gram N_A Not Available 20 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee d) Fish - smoked - cold-smoked - Processing plant - Bulgaria - food sample - batch 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Fishery products, unspecified - ready-to-eat - chilled - Processing plant - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Not Available - Not Available - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Foodstuffs intended for special nutritional uses - Processing plant - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Bulgaria - food sample - meat - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Foodstuffs intended for special nutritional uses - ready-to-eat - Processing single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Foodstuffs intended for special nutritional uses - ready-to-eat - Processing single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 plant - Bulgaria - food sample - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Fruits - pre-cut - ready-to-eat - Restaurant or Cafe or Pub or Bar or Hotel single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 or Catering service - Not Available - Not Available - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Fruits - pre-cut - ready-to-eat - Retail - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Fruits - Processing plant - Not Available - food sample - Surveillance - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Fruits - products - dried - Processing plant - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Fruits - products - dried - Processing plant - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Fruits - Retail - Not Available - food sample - Surveillance - Official single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 sampling - Objective sampling (food/fee 1:2017 d) Salmonella Juice - fruit juice - pasteurised - Processing plant - Bulgaria - food sample single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Juice - fruit juice - unpasteurised - Restaurant or Cafe or Pub or Bar or single 25 Gram N_A ISO 6579- 7 0 Salmonella 0 Hotel or Catering service - Bulgaria - food sample - Surveillance - based (food/fee 1:2017 on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Juice - fruit juice - unpasteurised - Restaurant or Cafe or Pub or Bar or single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Hotel or Catering service - Bulgaria - food sample - Surveillance - HACCP (food/fee 1:2017 and own check - Objective sampling d) Salmonella Juice - fruit juice - unpasteurised - Restaurant or Cafe or Pub or Bar or single 25 Millilitre N_A ISO 6579- 10 0 Salmonella 0 Hotel or Catering service - Bulgaria - food sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella

Bulgaria - 2019 53 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Juice - fruit juice - unpasteurised - Restaurant or Cafe or Pub or Bar or single 25 Gram N_A ISO 6579- 8 0 Salmonella 0 Hotel or Catering service - Not Available - Not Available - Surveillance - (food/fee 1:2017 based on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Juice - fruit juice - unpasteurised - Restaurant or Cafe or Pub or Bar or single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Hotel or Catering service - Not Available - Not Available - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Juice - fruit juice - unpasteurised - Retail - Bulgaria - food sample - meat - single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Juice - fruit juice - unpasteurised - Retail - Bulgaria - food sample - meat - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Juice - fruit juice - unpasteurised - Retail - Bulgaria - food sample - single 25 Millilitre N_A ISO 6579- 30 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella 40 0 Salmonella 0 Juice - fruit juice - unpasteurised - Retail - Not Available - food sample - single 25 Millilitre N_A ISO 6579- 30 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Juice - mixed juice - unpasteurised - Restaurant or Cafe or Pub or Bar or single 25 Millilitre N_A ISO 6579- 25 0 Salmonella 0 Hotel or Catering service - Bulgaria - food sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Live bivalve molluscs - mussels - Farm - Bulgaria - food sample - batch 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Live bivalve molluscs - mussels - Processing plant - Not Available - food single 25 Gram N_A ISO 6579- 35 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Live bivalve molluscs - mussels - Retail - Not Available - food sample - single 25 Gram N_A ISO 6579- 70 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Live bivalve molluscs - unspecified - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 60 0 Salmonella 0 sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Low alcoholic beverage prepared by fermented cereals - Processing plant - single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Low alcoholic beverage prepared by fermented cereals - Processing plant - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Bulgaria - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Low alcoholic beverage prepared by fermented cereals - Processing plant - single 25 Millilitre N_A ISO 6579- 3 0 Salmonella 0 Not Available - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Low alcoholic beverage prepared by fermented cereals - Retail - Not single 25 Millilitre N_A ISO 6579- 1 0 Salmonella 0 Available - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from bovine animals - carcase - Processing plant - Bulgaria - food slaughte 400 Square N_A ISO 6579- 7 0 Salmonella 0 sample - carcase swabs - Surveillance - HACCP and own check - Objective r animal centimetre 1:2017 sampling batch Salmonella Meat from bovine animals - carcase - Processing plant - Bulgaria - food slaughte 400 Square N_A ISO 6579- 11 0 Salmonella 0 sample - carcase swabs - Surveillance - Official sampling - Objective r animal centimetre 1:2017 sampling batch Salmonella Meat from bovine animals - carcase - Processing plant - Not Available - single 400 Square N_A ISO 6579- 116 0 Salmonella 0 Not Available - Surveillance - based on Regulation 2073 - Official sampling (food/fee centimetre 1:2017 - Objective sampling d) Salmonella Meat from bovine animals - carcase - Slaughterhouse - Bulgaria - food single 400 Square N_A ISO 6579- 81 0 Salmonella 0 sample - carcase swabs - Surveillance - based on Regulation 2073 - (food/fee centimetre 1:2017 Official sampling - Objective sampling d) Salmonella Meat from bovine animals - carcase - Slaughterhouse - Bulgaria - food single 400 Square N_A ISO 6579- 106 0 Salmonella 0 sample - carcase swabs - Surveillance - HACCP and own check - Objective (food/fee centimetre 1:2017 sampling d) Salmonella Meat from bovine animals - carcase - Slaughterhouse - Bulgaria - food single 400 Square N_A ISO 6579- 79 0 Salmonella 0 sample - carcase swabs - Surveillance - Official sampling - Objective (food/fee centimetre 1:2017 sampling d) Salmonella

Bulgaria - 2019 54 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from bovine animals - carcase - Slaughterhouse - Bulgaria - food single 400 Square N_A ISO 6579- 7 0 Salmonella 0 sample - Surveillance - HACCP and own check - Objective sampling (food/fee centimetre 1:2017 d) Salmonella Meat from bovine animals - carcase - Slaughterhouse - Bulgaria - food single 400 Square N_A ISO 6579- 5 0 Salmonella 0 sample - Surveillance - Official sampling - Objective sampling (food/fee centimetre 1:2017 d) Salmonella Meat from bovine animals - fresh - chilled - Processing plant - Not single 25 Gram N_A ISO 6579- 17 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from bovine animals - meat preparation - intended to be eaten single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 cooked - Processing plant - Bulgaria - food sample - Surveillance - HACCP (food/fee 1:2017 and own check - Objective sampling d) Salmonella Meat from bovine animals - meat preparation - intended to be eaten single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 cooked - Processing plant - Bulgaria - food sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from bovine animals - meat preparation - intended to be eaten single 10 Gram N_A ISO 6579- 3 0 Salmonella 0 cooked - Retail - Bulgaria - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from bovine animals - meat products - cooked, ready-to-eat - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from bovine animals - meat products - cooked, ready-to-eat - Retail single 25 Gram N_A ISO 6579- 100 0 Salmonella 0 - Bulgaria - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from bovine animals - meat products - fermented sausages - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from bovine animals - meat products - fermented sausages - single 25 Gram N_A ISO 6579- 11 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - HACCP and own (food/fee 1:2017 check - Objective sampling d) Salmonella Meat from bovine animals - meat products - fermented sausages - Retail - single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from bovine animals - meat products - fresh raw sausages - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Processing plant - Bulgaria - food sample - meat - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from bovine animals - meat products - raw but intended to be eaten single 10 Gram N_A ISO 6579- 10 0 Salmonella 0 cooked - Processing plant - Bulgaria - food sample - meat - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from bovine animals - meat products - ready-to-eat - Processing single 25 Gram N_A ISO 6579- 11 0 Salmonella 0 plant - Not Available - Not Available - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Meat from bovine animals - meat products - unspecified, ready-to-eat - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Processing plant - Not Available - Not Available - Surveillance - based on (food/fee 1:2017 Regulation 2073 - HACCP and own check - Objective sampling d) Salmonella Meat from bovine animals - meat products - unspecified, ready-to-eat - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Processing plant - Not Available - Not Available - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from bovine animals - meat products - unspecified, ready-to-eat - single 10 Gram N_A ISO 6579- 2 0 Salmonella 0 Retail - Not Available - Not Available - Surveillance - based on Regulation (food/fee 1:2017 2073 - Official sampling - Objective sampling d) Salmonella Meat from bovine animals - minced meat - intended to be eaten cooked - single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 chilled - Processing plant - Bulgaria - food sample - meat - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from bovine animals - minced meat - intended to be eaten cooked - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 chilled - Processing plant - Bulgaria - food sample - Surveillance - based (food/fee 1:2017 on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from bovine animals - minced meat - intended to be eaten cooked - batch 10 Gram N_A Slide 5 5 Salmonella Typhimurium 5 Processing plant - Bulgaria - food sample - Surveillance - HACCP and own (food/fee agglutination check - Objective sampling d) according White Kauffmann Le Minor Scheme

Bulgaria - 2019 55 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from bovine animals - minced meat - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 5 0 Salmonella 0 Processing plant - Not Available - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from bovine animals and pig - meat preparation - intended to be single 25 Gram N_A ISO 6579- 35 0 Salmonella 0 eaten cooked - Processing plant - Bulgaria - food sample - Surveillance - (food/fee 1:2017 HACCP and own check - Objective sampling d) Salmonella Meat from bovine animals and pig - meat preparation - intended to be single 25 Gram N_A ISO 6579- 140 0 Salmonella 0 eaten cooked - Processing plant - Bulgaria - food sample - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from bovine animals and pig - meat preparation - intended to be single 25 Gram N_A ISO 6579- 8 0 Salmonella 0 eaten cooked - Processing plant - Not Available - Not Available - (food/fee 1:2017 Surveillance - HACCP and own check - Objective sampling d) Salmonella Meat from bovine animals and pig - meat preparation - intended to be single 25 Gram N_A ISO 6579- 31 0 Salmonella 0 eaten cooked - Processing plant - Not Available - Not Available - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Meat from bovine animals and pig - meat products - Game handling batch 200 Gram N_A Not Available 312 0 Salmonella 0 establishment - Not Available - Not Available - Surveillance - HACCP and (food/fee own check - Objective sampling d) Meat from bovine animals and pig - meat products - ready-to-eat - single 25 Gram N_A ISO 6579- 35 0 Salmonella 0 Processing plant - Not Available - Not Available - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from bovine animals and pig - minced meat - intended to be eaten single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 cooked - Processing plant - Bulgaria - food sample - meat - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from bovine animals and pig - minced meat - intended to be eaten single 25 Gram N_A ISO 6579- 26 0 Salmonella 0 cooked - Processing plant - Not Available - Not Available - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - carcase - chilled - Slaughterhouse - single 25 Gram N_A ISO 6579- 19 0 Salmonella 0 Bulgaria - food sample - neck skin - Surveillance - based on Regulation (food/fee 1:2017 2073 - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - carcase - Processing plant - Not single 400 Square N_A ISO 6579- 25 0 Salmonella 0 Available - Not Available - Surveillance - based on Regulation 2073 - (food/fee centimetre 1:2017 Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - carcase - Slaughterhouse - Bulgaria - slaughte 25 Gram N_A ISO 6579- 70 3 Salmonella Infantis 2 food sample - neck skin - Surveillance - based on Regulation 2073 - r animal 1:2017 Salmonella Thompson 1 Official sampling - Objective sampling batch Salmonella Meat from broilers (Gallus gallus) - carcase - Slaughterhouse - Bulgaria - slaughte 25 Gram N_A ISO 6579- 11 0 Salmonella 0 food sample - neck skin - Surveillance - HACCP and own check - Objective r animal 1:2017 sampling batch Salmonella Meat from broilers (Gallus gallus) - carcase - Slaughterhouse - Bulgaria - slaughte 25 Gram N_A ISO 6579- 30 0 Salmonella 0 food sample - neck skin - Surveillance - Official sampling - Objective r animal 1:2017 sampling batch Salmonella Meat from broilers (Gallus gallus) - carcase - Slaughterhouse - Not slaughte Gram N_A Not Available 520 0 Salmonella 0 Available - Not Available - Surveillance - HACCP and own check - Objective r animal sampling batch Meat from broilers (Gallus gallus) - carcase - spent hens - Slaughterhouse single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 - Not Available - Not Available - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - fresh - chilled - Processing plant - single 25 Gram N_A Not Available 116 0 Salmonella 0 Bulgaria - food sample - meat - Surveillance - HACCP and own check - (food/fee Objective sampling d) Meat from broilers (Gallus gallus) - fresh - Processing plant - Bulgaria - single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 food sample - Surveillance - based on Regulation 2073 - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - fresh - Retail - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 21 1 Salmonella 0 eaten cooked - chilled - Cutting plant - Bulgaria - food sample - meat - (food/fee 1:2017 Salmonella Infantis 1 Surveillance - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 35 0 Salmonella 0 eaten cooked - chilled - Retail - Bulgaria - food sample - meat - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Bulgaria - 2019 56 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 30 0 Salmonella 0 eaten cooked - chilled - Slaughterhouse - Bulgaria - food sample - meat - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 25 0 Salmonella 0 eaten cooked - frozen - Cutting plant - Bulgaria - food sample - meat - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 eaten cooked - frozen - Slaughterhouse - Bulgaria - food sample - meat - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 50 0 Salmonella 0 eaten cooked - Processing plant - Bulgaria - food sample - meat - (food/fee 1:2017 Surveillance - HACCP and own check - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 eaten cooked - Processing plant - Bulgaria - food sample - meat - (food/fee 1:2017 170 0 Salmonella 0 Surveillance - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 394 2 Salmonella Infantis 2 eaten cooked - Processing plant - Bulgaria - food sample - Surveillance - (food/fee 1:2017 based on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 12 0 Salmonella 0 eaten cooked - Processing plant - Bulgaria - food sample - Surveillance - (food/fee 1:2017 HACCP and own check - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 50 0 Salmonella 0 eaten cooked - Processing plant - Not Available - food sample - (food/fee 1:2017 Surveillance - based on Regulation 2073 - Official sampling - Objective d) Salmonella sampling Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 eaten cooked - Processing plant - Not Available - Not Available - (food/fee 1:2017 Surveillance - based on Regulation 2073 - HACCP and own check - d) Salmonella Objective sampling Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 28 0 Salmonella 0 eaten cooked - Processing plant - Not Available - Not Available - (food/fee 1:2017 Surveillance - based on Regulation 2073 - Official sampling - Objective d) Salmonella sampling Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 30 0 Salmonella 0 eaten cooked - Retail - Bulgaria - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 115 0 Salmonella 0 eaten cooked - Retail - Not Available - food sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat preparation - intended to be single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 eaten cooked - Retail - Not Available - Not Available - Surveillance - based (food/fee 1:2017 on Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Processing plant - Not Available - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat products - cooked, ready-to-eat - single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 Restaurant or Cafe or Pub or Bar or Hotel or Catering service - Bulgaria - (food/fee 1:2017 food sample - meat - Surveillance - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat products - raw but intended to single 25 Gram N_A ISO 6579- 55 0 Salmonella 0 be eaten cooked - Processing plant - Bulgaria - food sample - meat - (food/fee 1:2017 Surveillance - HACCP and own check - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - meat products - raw but intended to single 25 Gram N_A ISO 6579- 85 0 Salmonella 0 be eaten cooked - Processing plant - Bulgaria - food sample - meat - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - mechanically separated meat (MSM) - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Slaughterhouse - Bulgaria - food sample - meat - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - offal - Processing plant - Bulgaria - single 25 Gram N_A ISO 6579- 1 1 Salmonella Yarm 1 food sample - Surveillance - based on Regulation 2073 - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Meat from broilers (Gallus gallus) - offal - Processing plant - Bulgaria - single 25 Gram N_A ISO 6579- 8 0 Salmonella 0 food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella

Bulgaria - 2019 57 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from broilers (Gallus gallus) - Processing plant - Not Available - Not batch 25 Gram N_A Not Available 1984 0 Salmonella 0 Available - Surveillance - HACCP and own check - Objective sampling (food/fee d) Meat from deer (venison) - meat preparation - intended to be eaten single 10 Gram N_A ISO 6579- 1 0 Salmonella 0 cooked - Processing plant - Bulgaria - food sample - Surveillance - HACCP (food/fee 1:2017 and own check - Objective sampling d) Salmonella Meat from deer (venison) - minced meat - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 2 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - HACCP and own (food/fee 1:2017 check - Objective sampling d) Salmonella Meat from duck - carcase - chilled - Processing plant - Bulgaria - food batch 25 Gram N_A Slide 4 4 Salmonella Thompson 4 sample - Surveillance - HACCP and own check - Objective sampling (food/fee agglutination d) according White Kauffmann Le Minor Scheme Meat from duck - carcase - Slaughterhouse - Bulgaria - food sample - neck slaughte 25 Gram N_A ISO 6579- 4 0 Salmonella 0 skin - Surveillance - based on Regulation 2073 - Official sampling - r animal 1:2017 Objective sampling batch Salmonella Meat from duck - carcase - Slaughterhouse - Not Available - food sample - single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 neck skin - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Meat from duck - carcase - Slaughterhouse - Not Available - Not Available slaughte Gram N_A Not Available 120 0 Salmonella 0 - Surveillance - HACCP and own check - Objective sampling r animal batch Meat from duck - fresh - chilled - Processing plant - Bulgaria - food sample single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from duck - meat preparation - intended to be eaten cooked - chilled single 25 Gram N_A ISO 6579- 8 0 Salmonella 0 - Processing plant - Not Available - Not Available - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from duck - meat preparation - intended to be eaten cooked - single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Processing plant - Not Available - Not Available - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from duck - meat products - raw but intended to be eaten cooked - single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 chilled - Processing plant - Not Available - Not Available - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from duck - meat products - ready-to-eat - Processing plant - Not single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from duck - minced meat - Processing plant - Not Available - food single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Meat from duck - offal - liver - chilled - Processing plant - Not Available - single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Not Available - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Meat from duck - offal - Processing plant - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from duck - offal - Processing plant - Not Available - food sample - single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from duck - Processing plant - Not Available - Not Available - batch 25 Gram N_A Not Available 997 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee d) Meat from pig - carcase - chilled - Processing plant - Bulgaria - food batch 400 Square N_A Slide 5 1 Salmonella Senftenberg 1 sample - Surveillance - based on Regulation 2073 - HACCP and own check (food/fee centimetre agglutination - Objective sampling d) according White Kauffmann Le Minor Scheme Meat from pig - carcase - chilled - Slaughterhouse - Bulgaria - food sample single 400 Square N_A ISO 6579- 927 0 Salmonella 0 - carcase swabs - Surveillance - based on Regulation 2073 - Official, based (food/fee centimetre 1:2017 on Regulation 854/2004 - Objective sampling d) Salmonella

Bulgaria - 2019 58 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from pig - carcase - Processing plant - Bulgaria - food sample - slaughte 400 Square N_A ISO 6579- 9 0 Salmonella 0 carcase swabs - Surveillance - based on Regulation 2073 - HACCP and r animal centimetre 1:2017 own check - Objective sampling batch Salmonella Meat from pig - carcase - Processing plant - Bulgaria - food sample - slaughte 400 Square N_A ISO 6579- 30 0 Salmonella 0 carcase swabs - Surveillance - based on Regulation 2073 - Official, based r animal centimetre 1:2017 on Regulation 854/2004 - Objective sampling batch Salmonella Meat from pig - carcase - Processing plant - Not Available - food sample - single 400 Square N_A ISO 6579- 260 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official, based on Regulation (food/fee centimetre 1:2017 854/2004 - Objective sampling d) Salmonella Meat from pig - carcase - Slaughterhouse - Bulgaria - food sample - single 400 Square N_A ISO 6579- 242 0 Salmonella 0 carcase swabs - Surveillance - based on Regulation 2073 - HACCP and (food/fee centimetre 1:2017 own check - Objective sampling d) Salmonella Meat from pig - carcase - Slaughterhouse - Bulgaria - food sample - single 400 Square N_A ISO 6579- 304 0 Salmonella 0 carcase swabs - Surveillance - based on Regulation 2073 - Official, based (food/fee centimetre 1:2017 836 0 Salmonella 0 d) Salmonella on Regulation 854/2004 - Objective sampling 840 0 Salmonella 0 Meat from pig - carcase - Slaughterhouse - Bulgaria - food sample - batch 400 Square N_A ISO 6579- 35 0 Salmonella 0 Surveillance - based on Regulation 2073 - HACCP and own check - (food/fee centimetre 1:2017 Objective sampling d) Salmonella Meat from pig - carcase - Slaughterhouse - Bulgaria - food sample - batch 400 Square N_A ISO 6579- 41 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official, based on Regulation (food/fee centimetre 1:2017 854/2004 - Objective sampling d) Salmonella Meat from pig - carcase - Slaughterhouse - Not Available - food sample - single 400 Square N_A ISO 6579- 95 0 Salmonella 0 Surveillance - based on Regulation 2073 - HACCP and own check - (food/fee centimetre 1:2017 Objective sampling d) Salmonella Meat from pig - carcase - Slaughterhouse - Not Available - food sample - single 400 Square N_A ISO 6579- 114 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official, based on Regulation (food/fee centimetre 1:2017 854/2004 - Objective sampling d) Salmonella Meat from pig - fresh - chilled - Processing plant - Bulgaria - food sample - single 25 Gram N_A Slide 1 1 Salmonella Infantis 1 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee agglutination sampling d) according White Kauffmann Le Minor Scheme Meat from pig - fresh - chilled - Processing plant - Bulgaria - food sample - single 25 Gram N_A Slide 1 1 Salmonella Typhimurium, 1 Surveillance - Official sampling - Objective sampling (food/fee agglutination monophasic d) according White Kauffmann Le Minor Scheme Meat from pig - fresh - chilled - Processing plant - Not Available - food single 10 Gram N_A ISO 6579- 20 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Meat from pig - fresh - chilled - Processing plant - Not Available - Not single 25 Gram N_A ISO 6579- 14 0 Salmonella 0 Available - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Meat from pig - fresh - chilled - Retail - Bulgaria - food sample - single 25 Gram N_A Slide 1 1 Salmonella Hiduddify 1 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee agglutination sampling d) according White Salmonella Rissen 1 Kauffmann Le Minor Scheme Meat from pig - fresh - chilled - Retail - Not Available - food sample - single 10 Gram N_A ISO 6579- 45 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Meat from pig - fresh - Processing plant - Bulgaria - food sample - meat - single 10 Gram N_A ISO 6579- 13 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - fresh - Processing plant - Bulgaria - food sample - meat - single 10 Gram N_A ISO 6579- 1 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Meat from pig - fresh - Processing plant - Bulgaria - food sample - single 10 Gram N_A ISO 6579- 20 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella

Bulgaria - 2019 59 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from pig - fresh - Retail - Bulgaria - food sample - meat - single 10 Gram N_A ISO 6579- 4 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - meat preparation - intended to be eaten cooked - chilled - single 10 Gram N_A ISO 6579- 60 0 Salmonella 0 Processing plant - Bulgaria - food sample - meat - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella 25 Gram N_A ISO 6579- 45 0 Salmonella 0 1:2017 Salmonella Meat from pig - meat preparation - intended to be eaten cooked - chilled - single 25 Gram N_A Slide 2 2 Salmonella Derby 2 Processing plant - Bulgaria - food sample - Surveillance - based on (food/fee agglutination Regulation 2073 - Official sampling - Objective sampling d) according White Kauffmann Le Minor Scheme Meat from pig - meat preparation - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 264 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from pig - meat preparation - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 5 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - HACCP and own (food/fee 1:2017 check - Objective sampling d) Salmonella 25 Gram N_A ISO 6579- 15 0 Salmonella 0 1:2017 Salmonella Meat from pig - meat preparation - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 5 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella 25 Gram N_A ISO 6579- 80 0 Salmonella 0 1:2017 Salmonella Meat from pig - meat preparation - intended to be eaten cooked - Retail - single 10 Gram N_A ISO 6579- 145 0 Salmonella 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from pig - meat preparation - Processing plant - Not Available - Not single 10 Gram N_A ISO 6579- 20 0 Salmonella 0 Available - Surveillance - based on Regulation 2073 - HACCP and own (food/fee 1:2017 check - Objective sampling d) Salmonella Meat from pig - meat preparation - Processing plant - Not Available - Not single 10 Gram N_A ISO 6579- 20 0 Salmonella 0 Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Meat from pig - meat preparation - Retail - Not Available - Not Available - single 10 Gram N_A ISO 6579- 30 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - meat products - cooked ham - Processing plant - Not single 25 Gram N_A ISO 6579- 14 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - meat products - cooked, ready-to-eat - Processing plant - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - HACCP (food/fee 1:2017 and own check - Objective sampling d) Salmonella Meat from pig - meat products - cooked, ready-to-eat - Processing plant - single 25 Gram N_A ISO 6579- 130 0 Salmonella 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from pig - meat products - cooked, ready-to-eat - Processing plant - single 25 Gram N_A ISO 6579- 25 0 Salmonella 0 Not Available - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from pig - meat products - cooked, ready-to-eat - Processing plant - single 25 Gram N_A ISO 6579- 18 0 Salmonella 0 Not Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - meat products - cooked, ready-to-eat - Retail - Bulgaria - single 25 Gram N_A ISO 6579- 105 0 Salmonella 0 food sample - Surveillance - based on Regulation 2073 - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Meat from pig - meat products - fermented sausages - Processing plant - single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 Bulgaria - food sample - meat - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella

Bulgaria - 2019 60 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from pig - meat products - fermented sausages - Processing plant - single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from pig - meat products - fermented sausages - Processing plant - single 25 Gram N_A ISO 6579- 12 0 Salmonella 0 Bulgaria - food sample - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - meat products - fermented sausages - Retail - Bulgaria - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 food sample - Surveillance - based on Regulation 2073 - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Meat from pig - meat products - fresh raw sausages - Processing plant - single 25 Gram N_A ISO 6579- 55 0 Salmonella 0 Bulgaria - food sample - meat - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - meat products - fresh raw sausages - Processing plant - batch 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Bulgaria - food sample - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - meat products - Processing plant - Not Available - food single 25 Gram N_A ISO 6579- 25 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Meat from pig - meat products - raw and intended to be eaten raw - single 25 Gram N_A ISO 6579- 1 1 Salmonella Infantis 1 frozen - Processing plant - Bulgaria - food sample - meat - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from pig - meat products - raw but intended to be eaten cooked - single 25 Gram N_A Not Available 220 0 Salmonella 0 chilled - Processing plant - Bulgaria - food sample - meat - Surveillance - (food/fee HACCP and own check - Objective sampling d) Meat from pig - meat products - raw but intended to be eaten cooked - single 10 Gram N_A ISO 6579- 20 0 Salmonella 0 chilled - Processing plant - Bulgaria - food sample - meat - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella 25 Gram N_A ISO 6579- 10 0 Salmonella 0 1:2017 Salmonella Meat from pig - meat products - raw but intended to be eaten cooked - single 10 Gram N_A ISO 6579- 15 0 Salmonella 0 chilled - Retail - Bulgaria - food sample - meat - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from pig - meat products - raw but intended to be eaten cooked - single 25 Gram N_A ISO 6579- 10 1 Salmonella 1 frozen - Processing plant - Bulgaria - food sample - meat - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from pig - meat products - raw but intended to be eaten cooked - single 10 Gram N_A ISO 6579- 130 0 Salmonella 0 Processing plant - Not Available - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat from pig - meat products - raw but intended to be eaten cooked - single 10 Gram N_A ISO 6579- 45 0 Salmonella 0 Retail - Not Available - food sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Meat from pig - meat products - ready-to-eat - Processing plant - Not single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - meat products - Retail - Not Available - food sample - single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Meat from pig - meat products - unspecified, ready-to-eat - Processing single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 plant - Not Available - Not Available - Surveillance - based on Regulation (food/fee 1:2017 2073 - HACCP and own check - Objective sampling d) Salmonella Meat from pig - meat products - unspecified, ready-to-eat - Processing single 25 Gram N_A ISO 6579- 28 0 Salmonella 0 plant - Not Available - Not Available - Surveillance - based on Regulation (food/fee 1:2017 2073 - Official sampling - Objective sampling d) Salmonella Meat from pig - meat products - unspecified, ready-to-eat - Retail - Not single 25 Gram N_A ISO 6579- 13 0 Salmonella 0 Available - Not Available - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - chilled - single 10 Gram N_A ISO 6579- 15 0 Salmonella 0 Processing plant - Bulgaria - food sample - meat - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella 25 Gram N_A ISO 6579- 75 0 Salmonella 0 1:2017 Salmonella

Bulgaria - 2019 61 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from pig - minced meat - intended to be eaten cooked - chilled - single 25 Gram N_A ISO 6579- 13 0 Salmonella 0 Processing plant - Not Available - Not Available - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - chilled - single 10 Gram N_A ISO 6579- 10 0 Salmonella 0 Retail - Bulgaria - food sample - meat - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - chilled - single 25 Gram N_A Slide 1 1 Salmonella Enteritidis 1 Retail - Bulgaria - food sample - Surveillance - based on Regulation 2073 - (food/fee agglutination Official sampling - Objective sampling d) according White Kauffmann Le Minor Scheme Meat from pig - minced meat - intended to be eaten cooked - Cutting plant single 10 Gram N_A ISO 6579- 2 0 Salmonella 0 - Bulgaria - food sample - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - frozen - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Processing plant - Bulgaria - food sample - meat - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - Processing single 10 Gram N_A ISO 6579- 35 0 Salmonella 0 plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 HACCP and own check - Objective sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - Processing single 10 Gram N_A ISO 6579- 136 0 Salmonella 0 plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - Processing single 10 Gram N_A ISO 6579- 40 0 Salmonella 0 plant - Not Available - food sample - Surveillance - based on Regulation (food/fee 1:2017 2073 - HACCP and own check - Objective sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - Processing single 10 Gram N_A ISO 6579- 205 0 Salmonella 0 plant - Not Available - food sample - Surveillance - based on Regulation (food/fee 1:2017 2073 - Official sampling - Objective sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - Processing single 10 Gram N_A ISO 6579- 15 0 Salmonella 0 plant - Not Available - Not Available - Surveillance - based on Regulation (food/fee 1:2017 2073 - Official sampling - Objective sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - Retail - single 10 Gram N_A ISO 6579- 5 0 Salmonella 0 Bulgaria - food sample - meat - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - Retail - single 10 Gram N_A ISO 6579- 10 0 Salmonella 0 Bulgaria - food sample - meat - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - Retail - single 10 Gram N_A ISO 6579- 130 5 Salmonella 5 Bulgaria - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - Retail - Not single 10 Gram N_A ISO 6579- 122 0 Salmonella 0 Available - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from pig - minced meat - intended to be eaten cooked - Retail - Not single 10 Gram N_A ISO 6579- 10 0 Salmonella 0 Available - Not Available - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat from pig - minced meat - Processing plant - Not Available - Not single 25 Gram N_A ISO 6579- 26 0 Salmonella 0 Available - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Meat from poultry, unspecified - carcase - chilled - Processing plant - single 25 Gram N_A Slide 2 2 Salmonella Infantis 2 Bulgaria - food sample - neck skin - Surveillance - based on Regulation (food/fee agglutination 2073 - Official sampling - Objective sampling d) according White Kauffmann Le Minor Scheme Meat from poultry, unspecified - carcase - chilled - Processing plant - batch 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Bulgaria - food sample - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella

Bulgaria - 2019 62 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from poultry, unspecified - carcase - chilled - Processing plant - batch 25 Gram N_A Slide 4 4 Salmonella Thompson 4 Bulgaria - food sample - Surveillance - HACCP and own check - Objective (food/fee agglutination sampling d) according White Kauffmann Le Minor Scheme Meat from poultry, unspecified - carcase - chilled - Slaughterhouse - slaughte 25 Gram N_A Slide 30 4 Salmonella Infantis 4 Bulgaria - food sample - neck skin - Surveillance - based on Regulation r animal agglutination 2073 - Official sampling - Objective sampling batch according White Kauffmann Le Minor Scheme Meat from poultry, unspecified - carcase - Processing plant - Bulgaria - single 25 Gram N_A Slide 2 1 Salmonella Typhimurium 1 food sample - Surveillance - based on Regulation 2073 - Official sampling (food/fee agglutination - Objective sampling d) according White Kauffmann Le Minor Scheme Meat from poultry, unspecified - fresh - chilled - Processing plant - Not single 25 Gram N_A ISO 6579- 11 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from poultry, unspecified - fresh - chilled - Slaughterhouse - Bulgaria single 25 Gram N_A Slide 1 1 Salmonella Infantis 1 - food sample - Surveillance - based on Regulation 2073 - Official (food/fee agglutination sampling - Objective sampling d) according White Kauffmann Le Minor Scheme Meat from poultry, unspecified - meat preparation - intended to be eaten single 25 Gram N_A ISO 6579- 21 0 Salmonella 0 cooked - chilled - Processing plant - Not Available - Not Available - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Meat from poultry, unspecified - meat preparation - intended to be eaten single 25 Gram N_A ISO 6579- 8 0 Salmonella 0 cooked - frozen - Processing plant - Not Available - Not Available - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Meat from poultry, unspecified - meat preparation - intended to be eaten single 25 Gram N_A Slide 1 1 Salmonella Coeln 1 cooked - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - (food/fee agglutination Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official d) according sampling - Objective sampling White Kauffmann Le Minor Scheme Meat from poultry, unspecified - meat preparation - Wholesale - Poland - single 25 Gram N_A Slide 2 1 Salmonella Thompson 1 food sample - Surveillance - based on Regulation 2073 - Official sampling (food/fee agglutination - Objective sampling d) according White Kauffmann Le Minor Scheme Meat from poultry, unspecified - meat products - cooked, ready-to-eat - single 25 Gram N_A Slide 1 1 Salmonella Infantis 1 Catering - Bulgaria - food sample - Surveillance - based on Regulation (food/fee agglutination 2073 - Official sampling - Objective sampling d) according White Kauffmann Le Minor Scheme Meat from poultry, unspecified - meat products - raw but intended to be single 25 Gram N_A Slide 6 6 Salmonella Infantis 6 eaten cooked - chilled - Processing plant - Bulgaria - food sample - (food/fee agglutination Surveillance - based on Regulation 2073 - Official sampling - Objective d) according sampling White Kauffmann Le Minor Scheme Meat from poultry, unspecified - meat products - raw but intended to be single 25 Gram N_A ISO 6579- 9 0 Salmonella 0 eaten cooked - chilled - Processing plant - Not Available - Not Available - (food/fee 1:2017 Surveillance - HACCP and own check - Objective sampling d) Salmonella Meat from poultry, unspecified - meat products - raw but intended to be single 25 Gram N_A Slide 1 1 Salmonella Infantis 1 eaten cooked - chilled - Retail - Bulgaria - food sample - Surveillance - (food/fee agglutination based on Regulation 2073 - Official sampling - Objective sampling d) according White Kauffmann Le Minor Scheme

Bulgaria - 2019 63 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from poultry, unspecified - meat products - raw but intended to be single 25 Gram N_A Slide 3 3 Salmonella Infantis 3 eaten cooked - frozen - Processing plant - Bulgaria - food sample - (food/fee agglutination Surveillance - based on Regulation 2073 - Official sampling - Objective d) according sampling White Kauffmann Le Minor Scheme Meat from poultry, unspecified - meat products - raw but intended to be batch 25 Gram N_A ISO 6579- 5 0 Salmonella 0 eaten cooked - frozen - Processing plant - Bulgaria - food sample - (food/fee 1:2017 Surveillance - HACCP and own check - Objective sampling d) Salmonella Meat from poultry, unspecified - meat products - raw but intended to be single 25 Gram N_A Slide 3 3 Salmonella Enteritidis 2 eaten cooked - Retail - Bulgaria - food sample - Surveillance - based on (food/fee agglutination Regulation 2073 - Official sampling - Objective sampling d) according Salmonella Typhimurium, 1 White monophasic Kauffmann Le Minor Scheme Meat from poultry, unspecified - meat products - raw but intended to be single 25 Gram N_A Slide 3 2 Salmonella Enteritidis 1 eaten cooked - Wholesale - Bulgaria - food sample - Surveillance - based (food/fee agglutination on Regulation 2073 - Official sampling - Objective sampling d) according White Salmonella Kottbus 1 Kauffmann Le Minor Scheme Meat from poultry, unspecified - mechanically separated meat (MSM) - single 25 Gram N_A Slide 1 1 Salmonella Infantis 1 Processing plant - Bulgaria - food sample - Surveillance - based on (food/fee agglutination Regulation 2073 - Official sampling - Objective sampling d) according White Kauffmann Le Minor Scheme Meat from poultry, unspecified - offal - liver - frozen - Border Control Posts single 25 Gram N_A ISO 6579- 40 0 Salmonella 0 - Brazil - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from poultry, unspecified - offal - liver - Processing plant - Not single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat from sheep - carcase - chilled - Slaughterhouse - Bulgaria - food single 400 Square N_A ISO 6579- 45 0 Salmonella 0 sample - carcase swabs - Surveillance - based on Regulation 2073 - (food/fee centimetre 1:2017 Official sampling - Objective sampling d) Salmonella 401 Square N_A ISO 6579- 1 0 Salmonella 0 centimetre 1:2017 Salmonella Meat from sheep - carcase - Processing plant - Not Available - Not single 400 Square N_A ISO 6579- 74 0 Salmonella 0 Available - Surveillance - based on Regulation 2073 - Official sampling - (food/fee centimetre 1:2017 Objective sampling d) Salmonella Meat from sheep - carcase - Slaughterhouse - Bulgaria - food sample - single 400 Square N_A ISO 6579- 30 0 Salmonella 0 carcase swabs - Surveillance - based on Regulation 2073 - Official (food/fee centimetre 1:2017 sampling - Objective sampling d) Salmonella Meat from sheep - carcase - Slaughterhouse - Bulgaria - food sample - single 400 Square N_A ISO 6579- 5 0 Salmonella 0 carcase swabs - Surveillance - HACCP and own check - Objective sampling (food/fee centimetre 1:2017 d) Salmonella Meat from sheep - carcase - Slaughterhouse - Bulgaria - food sample - single 400 Square N_A ISO 6579- 55 0 Salmonella 0 carcase swabs - Surveillance - Official sampling - Objective sampling (food/fee centimetre 1:2017 d) Salmonella Meat from sheep - carcase - Slaughterhouse - Bulgaria - food sample - single 400 Square N_A ISO 6579- 6 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee centimetre 1:2017 d) Salmonella Meat from sheep - carcase - Slaughterhouse - Bulgaria - food sample - single 400 Square N_A ISO 6579- 6 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee centimetre 1:2017 d) Salmonella Meat from sheep - carcase - Slaughterhouse - Not Available - Not single 25 Square N_A ISO 6579- 5 0 Salmonella 0 Available - Surveillance - HACCP and own check - Objective sampling (food/fee centimetre 1:2017 d) Salmonella Meat from turkey - meat products - raw but intended to be eaten cooked - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Processing plant - Bulgaria - food sample - meat - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat from wild boar - minced meat - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 1 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella

Bulgaria - 2019 64 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from wild boar - minced meat - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 5 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - HACCP and own (food/fee 1:2017 check - Objective sampling d) Salmonella Meat, mixed meat - meat preparation - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 76 2 Salmonella Infantis 1 Processing plant - Bulgaria - food sample - Surveillance - based on (food/fee 1:2017 Salmonella Typhimurium, 1 Regulation 2073 - Official sampling - Objective sampling d) Salmonella monophasic Meat, mixed meat - meat preparation - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 27 0 Salmonella 0 Processing plant - Bulgaria - food sample - Surveillance - HACCP and own (food/fee 1:2017 check - Objective sampling d) Salmonella Meat, mixed meat - meat preparation - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 49 1 Salmonella Typhimurium, 1 Retail - Bulgaria - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 monophasic Official sampling - Objective sampling d) Salmonella Meat, mixed meat - meat preparation - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 9 2 Salmonella Brandenburg 1 Wholesale - Bulgaria - food sample - Surveillance - based on Regulation (food/fee 1:2017 Salmonella Infantis 1 2073 - Official sampling - Objective sampling d) Salmonella Meat, mixed meat - meat products - cooked, ready-to-eat - Processing single 25 Gram N_A ISO 6579- 7 0 Salmonella 0 plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat, mixed meat - meat products - cooked, ready-to-eat - Retail - single 25 Gram N_A ISO 6579- 25 0 Salmonella 0 Bulgaria - food sample - meat - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat, mixed meat - meat products - fermented sausages - Game handling batch 200 Gram N_A Not Available 243 0 Salmonella 0 establishment - Not Available - Not Available - Surveillance - HACCP and (food/fee own check - Objective sampling d) Meat, mixed meat - meat products - fermented sausages - Processing single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 plant - Bulgaria - food sample - meat - Surveillance - HACCP and own (food/fee 1:2017 check - Objective sampling d) Salmonella Meat, mixed meat - meat products - fermented sausages - Processing single 25 Gram N_A ISO 6579- 21 2 Salmonella 2 plant - Bulgaria - food sample - meat - Surveillance - Official sampling - (food/fee 1:2017 30 0 Salmonella 0 Objective sampling d) Salmonella Meat, mixed meat - meat products - fermented sausages - Processing single 25 Gram N_A ISO 6579- 18 0 Salmonella 0 plant - Bulgaria - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat, mixed meat - meat products - fermented sausages - Processing single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 plant - Bulgaria - food sample - Surveillance - HACCP and own check - (food/fee 1:2017 31 0 Salmonella 0 Objective sampling d) Salmonella Meat, mixed meat - meat products - fermented sausages - Processing single 25 Gram N_A ISO 6579- 30 0 Salmonella 0 plant - Bulgaria - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat, mixed meat - meat products - fermented sausages - Retail - single 25 Gram N_A ISO 6579- 30 0 Salmonella 0 Bulgaria - food sample - meat - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat, mixed meat - meat products - fermented sausages - Retail - single 25 Gram N_A ISO 6579- 8 0 Salmonella 0 Bulgaria - food sample - Surveillance - based on Regulation 2073 - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat, mixed meat - meat products - fresh raw sausages - Processing plant single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 - Bulgaria - food sample - meat - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Meat, mixed meat - meat products - fresh raw sausages - Processing plant single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 - Bulgaria - food sample - meat - Surveillance - Official sampling - (food/fee 1:2017 15 0 Salmonella 0 Objective sampling d) Salmonella Meat, mixed meat - meat products - fresh raw sausages - Processing plant batch 25 Gram N_A ISO 6579- 10 0 Salmonella 0 - Bulgaria - food sample - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Meat, mixed meat - meat products - Processing plant - Bulgaria - food single 10 Gram N_A ISO 6579- 75 0 Salmonella 0 sample - meat - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Meat, mixed meat - meat products - Processing plant - Bulgaria - food single 10 Gram N_A ISO 6579- 275 0 Salmonella 0 sample - meat - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella 25 Gram N_A ISO 6579- 15 0 Salmonella 0 1:2017 Salmonella

Bulgaria - 2019 65 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat, mixed meat - meat products - raw but intended to be eaten cooked single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 - chilled - Processing plant - Bulgaria - food sample - meat - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Meat, mixed meat - meat products - Retail - Bulgaria - food sample - meat single 10 Gram N_A ISO 6579- 5 0 Salmonella 0 - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Meat, mixed meat - meat products - Retail - Bulgaria - food sample - meat single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Meat, mixed meat - minced meat - intended to be eaten cooked - chilled - single 25 Gram N_A ISO 6579- 55 0 Salmonella 0 Processing plant - Bulgaria - food sample - meat - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Meat, mixed meat - minced meat - intended to be eaten cooked - chilled - single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 Retail - Bulgaria - food sample - meat - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Meat, mixed meat - minced meat - intended to be eaten cooked - Game batch 200 Gram N_A Not Available 208 0 Salmonella 0 handling establishment - Not Available - Not Available - Surveillance - (food/fee HACCP and own check - Objective sampling d) Meat, mixed meat - minced meat - intended to be eaten cooked - single 10 Gram N_A ISO 6579- 15 0 Salmonella 0 Processing plant - Not Available - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Meat, mixed meat - minced meat - intended to be eaten cooked - Retail - single 10 Gram N_A ISO 6579- 10 0 Salmonella 0 Not Available - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Meat, mixed meat - minced meat - Retail - Bulgaria - food sample - meat - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Meat, mixed meat - minced meat - Retail - Bulgaria - food sample - meat - single 25 Gram N_A ISO 6579- 13 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Meat, mixed meat - Processing plant - Not Available - Not Available - batch 25 Gram N_A Not Available 8496 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee d) Meat, red meat (meat from bovines, pigs, goats, sheep, horses, donkeys, slaughte 400 Square N_A Not Available 2267 0 Salmonella 0 bison and water buffalos) - carcase - Slaughterhouse - Not Available - Not r animal centimetre Available - Surveillance - HACCP and own check - Objective sampling batch Milk, cows' - pasteurised milk - Game handling establishment - Not batch 200 Gram N_A Not Available 314 0 Salmonella 0 Available - Not Available - Surveillance - HACCP and own check - Objective (food/fee sampling d) Milk, cows' - pasteurised milk - Processing plant - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Milk, cows' - pasteurised milk - Processing plant - Not Available - food single 25 Millilitre N_A ISO 6579- 5 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Molluscan shellfish - cooked - chilled - Processing plant - Not Available - single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 food sample - Surveillance - based on Regulation 2073 - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Molluscan shellfish - cooked - frozen - Processing plant - Bulgaria - food single 25 Gram N_A Not Available 11 0 Salmonella 0 sample - Surveillance - HACCP and own check - Objective sampling (food/fee d) Molluscan shellfish - cooked - Processing plant - Not Available - food single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 sample - Surveillance - based on Regulation 2073 - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Molluscan shellfish - cooked - Retail - Not Available - food sample - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Molluscan shellfish - raw - Processing plant - Bulgaria - food sample - single 25 Gram N_A Not Available 34 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee d) Molluscan shellfish - raw - Processing plant - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella

Bulgaria - 2019 66 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Nuts and nut products - Packing centre - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Nuts and nut products - Retail - Bulgaria - food sample - meat - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Other processed food products and prepared dishes - Catering - Bulgaria - single 25 Gram N_A ISO 6579- 61 0 Salmonella 0 food sample - Surveillance - based on Regulation 2073 - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Other processed food products and prepared dishes - Catering - Bulgaria - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 food sample - Surveillance - based on Regulation 2073 - Official sampling (food/fee 1:2017 - Suspect sampling d) Salmonella Other processed food products and prepared dishes - Catering - Bulgaria - single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Other processed food products and prepared dishes - fish and seafood single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 based dishes - Retail - Bulgaria - food sample - meat - Surveillance - (food/fee 1:2017 HACCP and own check - Objective sampling d) Salmonella Other processed food products and prepared dishes - fish and seafood single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 based dishes - Retail - Bulgaria - food sample - meat - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - meat based dishes - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Catering - Bulgaria - food sample - Surveillance - based on Regulation (food/fee 1:2017 2073 - Official sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - meat based dishes - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Catering - Bulgaria - food sample - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Other processed food products and prepared dishes - meat based dishes - single 25 Gram N_A ISO 6579- 39 0 Salmonella 0 Catering - Not Available - food sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Other processed food products and prepared dishes - meat based dishes - single 25 Gram N_A ISO 6579- 19 0 Salmonella 0 Processing plant - Not Available - Not Available - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - meat based dishes - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Restaurant or Cafe or Pub or Bar or Hotel or Catering service - Not (food/fee 1:2017 Available - food sample - Surveillance - HACCP and own check - Objective d) Salmonella sampling Other processed food products and prepared dishes - meat based dishes - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Restaurant or Cafe or Pub or Bar or Hotel or Catering service - Not (food/fee 1:2017 Available - food sample - Surveillance - Official sampling - Objective d) Salmonella sampling Other processed food products and prepared dishes - meat based dishes - single 25 Gram N_A ISO 6579- 11 0 Salmonella 0 Retail - Bulgaria - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - meat based dishes - single 25 Gram N_A ISO 6579- 12 0 Salmonella 0 Retail - Bulgaria - food sample - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Other processed food products and prepared dishes - meat based dishes - single 25 Gram N_A ISO 6579- 8 0 Salmonella 0 Retail - Bulgaria - food sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Other processed food products and prepared dishes - meat based dishes - single 25 Gram N_A ISO 6579- 13 0 Salmonella 0 Retail - Not Available - Not Available - Surveillance - HACCP and own (food/fee 1:2017 check - Objective sampling d) Salmonella Other processed food products and prepared dishes - potato based dishes single 25 Gram N_A ISO 6579- 7 0 Salmonella 0 - Processing plant - Not Available - food sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - Processing plant - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Not Available - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Other processed food products and prepared dishes - Restaurant or Cafe batch 25 Gram N_A Not Available 2463 0 Salmonella 0 or Pub or Bar or Hotel or Catering service - Not Available - Not Available - (food/fee Surveillance - HACCP and own check - Objective sampling d)

Bulgaria - 2019 67 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Other processed food products and prepared dishes - Retail - Bulgaria - single 25 Gram N_A ISO 6579- 13 0 Salmonella 0 food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella 15 0 Salmonella 0 Other processed food products and prepared dishes - Retail - Bulgaria - single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 food sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella 57 0 Salmonella 0 Other processed food products and prepared dishes - sandwiches - single 25 Gram N_A ISO 6579- 11 0 Salmonella 0 Processing plant - Not Available - food sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - sandwiches - single 25 Gram N_A ISO 6579- 17 0 Salmonella 0 Restaurant or Cafe or Pub or Bar or Hotel or Catering service - Bulgaria - (food/fee 1:2017 food sample - Surveillance - based on Regulation 2073 - Official sampling d) Salmonella - Objective sampling Other processed food products and prepared dishes - sandwiches - single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Restaurant or Cafe or Pub or Bar or Hotel or Catering service - Bulgaria - (food/fee 1:2017 food sample - Surveillance - HACCP and own check - Objective sampling d) Salmonella Other processed food products and prepared dishes - sandwiches - Retail - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Bulgaria - food sample - meat - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Other processed food products and prepared dishes - sandwiches - Retail - single 25 Gram N_A ISO 6579- 8 0 Salmonella 0 Bulgaria - food sample - meat - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Other processed food products and prepared dishes - sandwiches - Retail - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Bulgaria - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Other processed food products and prepared dishes - sandwiches - Retail - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Not Available - food sample - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Other processed food products and prepared dishes - sandwiches - Retail - single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 Not Available - food sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Other processed food products and prepared dishes - sandwiches - with single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 meat - Processing plant - Not Available - Not Available - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - sandwiches - with single 25 Gram N_A ISO 6579- 16 0 Salmonella 0 meat - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - (food/fee 1:2017 Bulgaria - food sample - Surveillance - Official sampling - Objective d) Salmonella sampling Other processed food products and prepared dishes - unspecified - ready- single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 to-eat foods - Restaurant or Cafe or Pub or Bar or Hotel or Catering (food/fee 1:2017 service - Bulgaria - food sample - Surveillance - based on Regulation 2073 d) Salmonella - Official sampling - Objective sampling Other processed food products and prepared dishes - unspecified - ready- single 25 Gram N_A ISO 6579- 17 0 Salmonella 0 to-eat foods - Retail - Not Available - Not Available - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - vegetable based single 25 Gram N_A ISO 6579- 8 0 Salmonella 0 dishes - Catering - Not Available - food sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Other processed food products and prepared dishes - vegetable based single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 dishes - Restaurant or Cafe or Pub or Bar or Hotel or Catering service - (food/fee 1:2017 Not Available - food sample - Surveillance - Official sampling - Objective d) Salmonella sampling Other processed food products and prepared dishes - vegetable based single 25 Gram N_A ISO 6579- 7 0 Salmonella 0 dishes - Retail - Bulgaria - food sample - Surveillance - HACCP and own (food/fee 1:2017 check - Objective sampling d) Salmonella Other processed food products and prepared dishes - vegetable based single 25 Gram N_A ISO 6579- 18 0 Salmonella 0 dishes - Retail - Bulgaria - food sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Other products of animal origin - gelatin and collagen - Packing centre - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Bulgaria - food sample - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Ready-to-eat salads - containing mayonnaise - Processing plant - Bulgaria single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 - food sample - meat - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Bulgaria - 2019 68 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Ready-to-eat salads - containing mayonnaise - Processing plant - Bulgaria single 25 Gram N_A ISO 6579- 11 0 Salmonella 0 - food sample - meat - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Ready-to-eat salads - containing mayonnaise - Processing plant - Not single 25 Gram N_A ISO 6579- 35 0 Salmonella 0 Available - food sample - Surveillance - based on Regulation 2073 - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Ready-to-eat salads - Processing plant - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Ready-to-eat salads - Processing plant - Not Available - food sample - single 25 Gram N_A ISO 6579- 25 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Sauce and dressings - mayonnaise - Processing plant - Bulgaria - food single 25 Gram N_A Not Available 45 0 Salmonella 0 sample - Surveillance - HACCP and own check - Objective sampling (food/fee d) Sauce and dressings - mayonnaise - Processing plant - Not Available - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 food sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Sauce and dressings - mayonnaise - Retail - Not Available - food sample - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Sauce and dressings - Retail - Not Available - food sample - Surveillance - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Seeds, dried - Border Control Posts - Brazil - food sample - Surveillance - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Seeds, dried - Border Control Posts - Ethiopia - food sample - Surveillance single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Seeds, dried - Border Control Posts - India - food sample - Surveillance - single 25 Gram N_A ISO 6579- 50 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Seeds, dried - Border Control Posts - Nigeria - food sample - Surveillance - single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Seeds, dried - Border Control Posts - Sudan - food sample - Surveillance - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Seeds, dried - Border Control Posts - Turkey - food sample - Surveillance - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Seeds, dried - Processing plant - Not Available - food sample - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Seeds, sprouted - ready-to-eat - Processing plant - Bulgaria - food sample batch 25 Gram N_A ISO 6579- 55 0 Salmonella 0 - Surveillance - based on Regulation 2073 - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Seeds, sprouted - ready-to-eat - Processing plant - Bulgaria - food sample single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 - Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Seeds, sprouted - ready-to-eat - Restaurant or Cafe or Pub or Bar or Hotel single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 or Catering service - Bulgaria - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Seeds, sprouted - ready-to-eat - Retail - Not Available - food sample - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Snails - Processing plant - Bulgaria - food sample - Surveillance - HACCP batch 25 Gram N_A ISO 6579- 1 0 Salmonella 0 and own check - Objective sampling (food/fee 1:2017 d) Salmonella Soups - ready-to-eat - Catering - Bulgaria - food sample - Surveillance - single 25 Gram N_A ISO 6579- 32 0 Salmonella 0 based on Regulation 2073 - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Soups - ready-to-eat - Catering - Bulgaria - food sample - Surveillance - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 based on Regulation 2073 - Official sampling - Suspect sampling (food/fee 1:2017 d) Salmonella Bulgaria - 2019 69 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Soups - ready-to-eat - Catering - Bulgaria - food sample - Surveillance - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Soups - ready-to-eat - Catering - Not Available - food sample - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Soups - ready-to-eat - Restaurant or Cafe or Pub or Bar or Hotel or single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Catering service - Not Available - food sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Soups - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - single 25 Gram N_A ISO 6579- 30 0 Salmonella 0 HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Soups - ready-to-eat - Retail - Bulgaria - food sample - Surveillance - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Soups - ready-to-eat - Retail - Not Available - Not Available - Surveillance single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Spices and herbs - dried - Border Control Posts - China - food sample - single 25 Gram N_A ISO 6579- 95 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Spices and herbs - dried - Packing centre - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 7 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Vegetables - pre-cut - frozen vegetables - Processing plant - Bulgaria - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 food sample - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Vegetables - pre-cut - frozen vegetables - Processing plant - Bulgaria - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 food sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Vegetables - pre-cut - ready-to-eat - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 sample - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Vegetables - pre-cut - ready-to-eat - Processing plant - Bulgaria - food single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Vegetables - pre-cut - ready-to-eat - Restaurant or Cafe or Pub or Bar or single 25 Gram N_A ISO 6579- 60 0 Salmonella 0 Hotel or Catering service - Bulgaria - food sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Vegetables - pre-cut - ready-to-eat - Restaurant or Cafe or Pub or Bar or single 25 Gram N_A ISO 6579- 30 0 Salmonella 0 Hotel or Catering service - Not Available - food sample - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Vegetables - pre-cut - ready-to-eat - Restaurant or Cafe or Pub or Bar or single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Hotel or Catering service - Not Available - Not Available - Surveillance - (food/fee 1:2017 Official sampling - Objective sampling d) Salmonella Vegetables - pre-cut - ready-to-eat - Retail - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Vegetables - pre-cut - ready-to-eat - Retail - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 60 0 Salmonella 0 d) Salmonella 65 0 Salmonella 0 Vegetables - pre-cut - ready-to-eat - Retail - Not Available - food sample - single 25 Gram N_A ISO 6579- 45 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Vegetables - pre-cut - Restaurant or Cafe or Pub or Bar or Hotel or single 25 Gram N_A ISO 6579- 13 0 Salmonella 0 Catering service - Bulgaria - food sample - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Vegetables - pre-cut - Restaurant or Cafe or Pub or Bar or Hotel or single 25 Gram N_A ISO 6579- 16 0 Salmonella 0 Catering service - Not Available - Not Available - Surveillance - based on (food/fee 1:2017 Regulation 2073 - Official sampling - Objective sampling d) Salmonella Vegetables - products - dried - Processing plant - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Surveillance - based on Regulation 2073 - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Vegetables - products - dried - Processing plant - Bulgaria - food sample - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Bulgaria - 2019 70 Table Salmonella:SALMONELLA in feed

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Complementary feedingstuffs - Feed mill - Bulgaria - feed sample - single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Complementary feedingstuffs - Feed mill - Bulgaria - feed sample - single 25 Gram N_A ISO 6579- 50 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Complementary feedingstuffs - final product - non-pelleted/meal - Farm - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Not Available - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Complementary feedingstuffs - final product - non-pelleted/meal - single 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Processing plant - Not Available - feed sample - Surveillance - HACCP and (food/fee 1:2017 own check - Objective sampling d) Salmonella Complementary feedingstuffs - final product - non-pelleted/meal - single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 Processing plant - Not Available - feed sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Complementary feedingstuffs - final product - pelleted - Processing plant - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Not Available - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Complementary feedingstuffs - final product - Processing plant - Bulgaria - batch 25 Gram N_A Slide 1 1 Salmonella Infantis 1 feed sample - Surveillance - HACCP and own check - Objective sampling (food/fee agglutination d) according White Salmonella Rissen 1 Kauffmann Le Minor Scheme Complementary feedingstuffs - final product - Processing plant - Bulgaria - batch 25 Gram N_A Slide 1 1 Salmonella Typhimurium 1 feed sample - Surveillance - Official sampling - Objective sampling (food/fee agglutination d) according White Kauffmann Le Minor Scheme single 25 Gram N_A Slide 1 1 Salmonella Agona 1 (food/fee agglutination d) according Salmonella Enteritidis 1 White Salmonella Rissen 1 Kauffmann Le Minor Scheme Salmonella Senftenberg 1 Complementary feedingstuffs - final product - Processing plant - Not single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Available - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Complementary feedingstuffs - final product - Processing plant - Not batch 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 (food/fee 1:2017 d) Salmonella Complementary feedingstuffs - process control - non-pelleted/meal - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Processing plant - Not Available - feed sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Complementary feedingstuffs - Processing plant - Not Available - Not batch 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Available - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Compound feedingstuffs for cattle - Feed mill - Bulgaria - feed sample - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Compound feedingstuffs for cattle - final product - Feed mill - Bulgaria - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 feed sample - Surveillance - Industry sampling - Objective sampling (food/fee 1:2017 d) Salmonella Compound feedingstuffs for cattle - final product - Feed mill - Bulgaria - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 feed sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Compound feedingstuffs for cattle - final product - non-pelleted/meal - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Processing plant - Not Available - feed sample - Surveillance - HACCP and (food/fee 1:2017 own check - Objective sampling d) Salmonella Bulgaria - 2019 71 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Compound feedingstuffs for cattle - final product - non-pelleted/meal - batch 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Processing plant - Not Available - Not Available - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Compound feedingstuffs for cattle - final product - Processing plant - single 25 Gram N_A ISO 6579- 75 3 Salmonella 0 Bulgaria - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 Salmonella Leeuwarden 1 d) Salmonella sampling Salmonella Rissen 2 Compound feedingstuffs for cattle - final product - Processing plant - single 25 Gram N_A Slide 1 1 Salmonella Leeuwarden 1 Bulgaria - feed sample - Surveillance - Official sampling - Objective (food/fee agglutination sampling d) according White Kauffmann Le Minor Scheme Compound feedingstuffs for cattle - final product - Processing plant - Not single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Compound feedingstuffs for fish - final product - pelleted - Processing single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 plant - Not Available - Not Available - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Compound feedingstuffs for fish - final product - Processing plant - Not batch 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Compound feedingstuffs for fish - Processing plant - Bulgaria - feed single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Compound feedingstuffs for pigs - Feed mill - Bulgaria - feed sample - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Compound feedingstuffs for pigs - final product - Feed mill - Bulgaria - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 feed sample - Surveillance - Industry sampling - Objective sampling (food/fee 1:2017 d) Salmonella Compound feedingstuffs for pigs - final product - Feed mill - Bulgaria - single 25 Gram N_A ISO 6579- 45 0 Salmonella 0 feed sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Compound feedingstuffs for pigs - final product - non-pelleted/meal - Farm single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 - Not Available - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Compound feedingstuffs for pigs - final product - non-pelleted/meal - single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Processing plant - Not Available - feed sample - Surveillance - HACCP and (food/fee 1:2017 own check - Objective sampling d) Salmonella Compound feedingstuffs for pigs - final product - non-pelleted/meal - single 25 Gram N_A ISO 6579- 80 0 Salmonella 0 Processing plant - Not Available - feed sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Compound feedingstuffs for pigs - final product - pelleted - Processing single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 plant - Not Available - feed sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Compound feedingstuffs for pigs - final product - Processing plant - single 25 Gram N_A ISO 6579- 45 0 Salmonella 0 Bulgaria - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Compound feedingstuffs for pigs - process control - non-pelleted/meal - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Processing plant - Not Available - feed sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Compound feedingstuffs for poultry (non specified) - final product - Feed single 25 Gram N_A ISO 6579- 75 0 Salmonella 0 mill - Bulgaria - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Compound feedingstuffs for poultry (non specified) - final product - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Processing plant - Not Available - feed sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Compound feedingstuffs for poultry, broilers - final product - non- single 25 Gram N_A ISO 6579- 17 0 Salmonella 0 pelleted/meal - Farm - Not Available - feed sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Compound feedingstuffs for poultry, broilers - final product - non- single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 pelleted/meal - Processing plant - Not Available - feed sample - (food/fee 1:2017 Surveillance - HACCP and own check - Objective sampling d) Salmonella

Bulgaria - 2019 72 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Compound feedingstuffs for poultry, broilers - final product - non- single 25 Gram N_A ISO 6579- 140 0 Salmonella 0 pelleted/meal - Processing plant - Not Available - feed sample - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Compound feedingstuffs for poultry, broilers - final product - pelleted - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Processing plant - Not Available - feed sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Compound feedingstuffs for poultry, broilers - final product - Processing single 25 Gram N_A ISO 6579- 100 0 Salmonella 0 plant - Bulgaria - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Compound feedingstuffs for poultry, broilers - final product - Processing batch 25 Gram N_A ISO 6579- 1 0 Salmonella 0 plant - Not Available - Not Available - Surveillance - HACCP and own check (food/fee 1:2017 - Objective sampling d) Salmonella Compound feedingstuffs for poultry, broilers - final product - Processing single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 plant - Not Available - Not Available - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Compound feedingstuffs for poultry, broilers - process control - non- single 25 Gram N_A ISO 6579- 65 0 Salmonella 0 pelleted/meal - Processing plant - Not Available - feed sample - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Compound feedingstuffs for poultry, laying hens - Feed mill - Bulgaria - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 feed sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Compound feedingstuffs for poultry, laying hens - final product - non- single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 pelleted/meal - Processing plant - Not Available - feed sample - (food/fee 1:2017 Surveillance - HACCP and own check - Objective sampling d) Salmonella Compound feedingstuffs for poultry, laying hens - final product - non- single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 pelleted/meal - Processing plant - Not Available - feed sample - (food/fee 1:2017 Surveillance - Official sampling - Objective sampling d) Salmonella Compound feedingstuffs for poultry, laying hens - final product - single 25 Gram N_A ISO 6579- 55 0 Salmonella 0 Processing plant - Bulgaria - feed sample - Surveillance - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Compound feedingstuffs for poultry, laying hens - final product - single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 Processing plant - Not Available - Not Available - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Compound feedingstuffs for poultry, laying hens - Processing plant - Not batch 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Compound feedingstuffs for poultry, pigeons - Processing plant - Not single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Available - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Compound feedingstuffs for sheep - Farm - Not Available - feed sample - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Compound feedingstuffs for sheep - final product - Processing plant - Not single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Compound feedingstuffs for turkeys - final product - Processing plant - Not batch 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Available - Not Available - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Compound feedingstuffs, not specified - final product - Feed mill - Bulgaria single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 - feed sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Feed material of cereal grain origin - barley derived - Feed mill - Bulgaria - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 feed sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Feed material of cereal grain origin - Farm - Bulgaria - feed sample - single 25 Gram N_A ISO 6579- 110 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Feed material of cereal grain origin - maize derived - Feed mill - Bulgaria - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 feed sample - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Feed material of cereal grain origin - maize derived - Feed mill - Bulgaria - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 feed sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella 40 0 Salmonella 0

Bulgaria - 2019 73 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Feed material of cereal grain origin - Processing plant - Bulgaria - feed single 25 Gram N_A ISO 6579- 40 0 Salmonella 0 sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Feed material of cereal grain origin - Processing plant - Not Available - Not batch 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Available - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Feed material of cereal grain origin - Processing plant - Not Available - Not batch 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Available - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Feed material of cereal grain origin - wheat derived - Feed mill - Bulgaria - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 feed sample - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Feed material of cereal grain origin - wheat derived - Feed mill - Bulgaria - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 feed sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Feed material of cereal grain origin - wheat derived - Processing plant - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Not Available - feed sample - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Feed material of land animal origin - dairy products - Farm - Bulgaria - single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 feed sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Feed material of land animal origin - Farm - Not Available - Not Available - batch 25 Gram N_A Not Available 5006 0 Salmonella 0 Surveillance - HACCP and own check - Objective sampling (food/fee d) Feed material of land animal origin - Feed mill - Not Available - Not batch 25 Gram N_A Not Available 126 0 Salmonella 0 Available - Surveillance - HACCP and own check - Objective sampling (food/fee d) Feed material of land animal origin - meat and bone meal - Processing single 25 Gram N_A ISO 6579- 26 1 Salmonella 1 plant - Bulgaria - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Feed material of land animal origin - protein meal - Processing plant - Not single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Available - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Feed material of marine animal origin - fish meal - Border Control Posts - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Denmark - feed sample - Surveillance - Industry sampling - Objective (food/fee 1:2017 sampling d) Salmonella Feed material of marine animal origin - fish meal - Border Control Posts - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Malaysia - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Feed material of marine animal origin - fish meal - Processing plant - Not single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 Available - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Feed material of oil seed or fruit origin - linseed derived - Processing plant single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 - Not Available - feed sample - Surveillance - HACCP and own check - (food/fee 1:2017 Objective sampling d) Salmonella Feed material of oil seed or fruit origin - linseed derived - Processing plant single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 - Not Available - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Feed material of oil seed or fruit origin - other oil seeds derived - Feed mill single 25 Gram N_A ISO 6579- 21 0 Salmonella 0 - Bulgaria - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Feed material of oil seed or fruit origin - rape seed derived - Processing single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 plant - Not Available - feed sample - Surveillance - HACCP and own check (food/fee 1:2017 - Objective sampling d) Salmonella Feed material of oil seed or fruit origin - rape seed derived - Processing single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 plant - Not Available - feed sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Feed material of oil seed or fruit origin - soya (bean) derived - Border single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Control Posts - Brazil - feed sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Feed material of oil seed or fruit origin - soya (bean) derived - Border single 25 Gram N_A ISO 6579- 30 0 Salmonella 0 Control Posts - Bulgaria - feed sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella

Bulgaria - 2019 74 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Feed material of oil seed or fruit origin - soya (bean) derived - Border single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 Control Posts - China - feed sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Feed material of oil seed or fruit origin - soya (bean) derived - Processing single 25 Gram N_A ISO 6579- 35 0 Salmonella 0 plant - Bulgaria - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Feed material of oil seed or fruit origin - soya (bean) derived - Processing single 25 Gram N_A ISO 6579- 2 0 Salmonella 0 plant - Not Available - feed sample - Surveillance - HACCP and own check (food/fee 1:2017 - Objective sampling d) Salmonella Feed material of oil seed or fruit origin - soya (bean) derived - Processing single 25 Gram N_A ISO 6579- 30 0 Salmonella 0 plant - Not Available - feed sample - Surveillance - Official sampling - (food/fee 1:2017 Objective sampling d) Salmonella Feed material of oil seed or fruit origin - sunflower seed derived - single 25 Gram N_A ISO 6579- 45 0 Salmonella 0 Processing plant - Bulgaria - feed sample - Surveillance - Official sampling (food/fee 1:2017 - Objective sampling d) Salmonella Feed material of oil seed or fruit origin - sunflower seed derived - single 25 Gram N_A ISO 6579- 1 0 Salmonella 0 Processing plant - Not Available - feed sample - Surveillance - HACCP and (food/fee 1:2017 own check - Objective sampling d) Salmonella Feed material of oil seed or fruit origin - sunflower seed derived - single 25 Gram N_A ISO 6579- 66 0 Salmonella 0 Processing plant - Not Available - feed sample - Surveillance - Official (food/fee 1:2017 sampling - Objective sampling d) Salmonella Other feed material - forages and roughages - Processing plant - Bulgaria single 25 Gram N_A ISO 6579- 30 0 Salmonella 0 - feed sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Other feed material - legume seeds and similar products - Processing plant single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 - Not Available - feed sample - Surveillance - Official sampling - Objective (food/fee 1:2017 sampling d) Salmonella Other feed material - other plants - Feed mill - Bulgaria - feed sample - single 25 Gram N_A ISO 6579- 8 0 Salmonella 0 Surveillance - Industry sampling - Objective sampling (food/fee 1:2017 d) Salmonella Other feed material - other plants - Feed mill - Bulgaria - feed sample - single 25 Gram N_A ISO 6579- 23 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Other feed material - plants - Processing plant - Not Available - feed single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Other feed material - straws - Processing plant - Not Available - feed single 25 Gram N_A ISO 6579- 10 0 Salmonella 0 sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Pet food - Feed mill - Bulgaria - feed sample - Surveillance - Official single 25 Gram N_A ISO 6579- 15 0 Salmonella 0 sampling - Objective sampling (food/fee 1:2017 d) Salmonella Pet food - final product - Border Control Posts - Canada - feed sample - single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Pet food - final product - Border Control Posts - China - feed sample - single 25 Gram N_A ISO 6579- 20 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Pet food - final product - Border Control Posts - United States - feed single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 sample - Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Pet food - final product - canned products - Processing plant - Not single 25 Gram N_A ISO 6579- 4 0 Salmonella 0 Available - Not Available - Surveillance - HACCP and own check - Objective (food/fee 1:2017 sampling d) Salmonella Pet food - final product - Feed mill - Bulgaria - feed sample - Surveillance - single 25 Gram N_A ISO 6579- 130 0 Salmonella 0 Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Pet food - final product - pelleted - Processing plant - Not Available - Not single 25 Gram N_A ISO 6579- 6 0 Salmonella 0 Available - Surveillance - HACCP and own check - Objective sampling (food/fee 1:2017 d) Salmonella Pet food - final product - Processing plant - Not Available - Not Available - batch 25 Gram N_A ISO 6579- 3 0 Salmonella 0 Surveillance - Official sampling - Objective sampling (food/fee 1:2017 d) Salmonella Pet food - Processing plant - Bulgaria - feed sample - Surveillance - HACCP single 25 Gram N_A ISO 6579- 5 0 Salmonella 0 and own check - Objective sampling (food/fee 1:2017 d) Salmonella Bulgaria - 2019 75 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Pet food - Retail - Bulgaria - feed sample - Surveillance - Official sampling single 25 Gram N_A ISO 6579- 165 0 Salmonella 0 - Objective sampling (food/fee 1:2017 d) Salmonella Premixtures - final product - Processing plant - Bulgaria - feed sample - single 25 Gram N_A Slide 2 2 Salmonella Enteritidis 2 Surveillance - Official sampling - Objective sampling (food/fee agglutination d) according White Kauffmann Le Minor Scheme

Bulgaria - 2019 76 Table Staphylococcal enterotoxins:STAPHYLOCOCCAL ENTEROTOXINS in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Cheeses made from cows' milk - curd - Processing plant - Bulgaria - food batch 25 Gram N_A ISO 40 0 Staphylococcal enterotoxins 0 sample - Surveillance - based on Regulation 2073 - HACCP and own check (food/fee 19020:2017 - Objective sampling d) Staphylococcal enterotoxins Cheeses made from cows' milk - hard - made from pasteurised milk - batch 25 Gram N_A ISO 30 0 Staphylococcal enterotoxins 0 Processing plant - Bulgaria - food sample - Surveillance - based on (food/fee 19020:2017 Regulation 2073 - HACCP and own check - Objective sampling d) Staphylococcal enterotoxins Cheeses made from cows' milk - soft and semi-soft - made from batch 25 Gram N_A ISO 40 0 Staphylococcal enterotoxins 0 pasteurised milk - Processing plant - Bulgaria - food sample - Surveillance (food/fee 19020:2017 - based on Regulation 2073 - HACCP and own check - Objective sampling d) Staphylococcal enterotoxins Cheeses, made from mixed milk from cows, sheep and/or goats - hard - batch 25 Gram N_A ISO 20 0 Staphylococcal enterotoxins 0 made from pasteurised milk - Processing plant - Bulgaria - food sample - (food/fee 19020:2017 Surveillance - based on Regulation 2073 - HACCP and own check - d) Staphylococcal Objective sampling enterotoxins Dairy products (excluding cheeses) - dairy products, not specified - ready- batch 25 Gram N_A ISO 30 0 Staphylococcal enterotoxins 0 to-eat - made from pasteurised milk - Processing plant - Bulgaria - food (food/fee 19020:2017 sample - Surveillance - based on Regulation 2073 - HACCP and own check d) Staphylococcal - Objective sampling enterotoxins Meat from poultry, unspecified - meat products - cooked, ready-to-eat - single 25 Gram N_A ISO 1 0 Staphylococcal enterotoxins 0 Retail - Bulgaria - food sample - Unspecified - Private sampling - (food/fee 19020:2017 Convenient sampling d) Staphylococcal enterotoxins

Bulgaria - 2019 77 Table Staphylococcus:STAPHYLOCOCCUS AUREUS METICILLIN RESISTANT (MRSA) in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Bakery products - cakes - Processing plant - Bulgaria - food sample - single 1 Gram N_A Not Available 24 0 Staphylococcus aureus 0 Surveillance - HACCP and own check - Objective sampling (food/fee d) Cheeses made from cows' milk - hard - Processing plant - Bulgaria - food single 1 Gram N_A Not Available 17 0 Staphylococcus aureus 0 sample - milk - Surveillance - HACCP and own check - Objective sampling (food/fee d) Cheeses made from cows' milk - Processing plant - Bulgaria - food sample single 1 Gram N_A Not Available 8 0 Staphylococcus aureus 0 - milk - Surveillance - HACCP and own check - Objective sampling (food/fee d) Cheeses made from cows' milk - soft and semi-soft - made from single 1 Gram N_A Not Available 12 0 Staphylococcus aureus 0 pasteurised milk - Processing plant - Bulgaria - food sample - milk - (food/fee Surveillance - HACCP and own check - Objective sampling d) Cheeses made from cows' milk - soft and semi-soft - made from single 1 Gram N_A EN ISO 6888- 23 0 Staphylococcus 0 pasteurised milk - Processing plant - Not Available - Not Available - (food/fee 2:1999/Amdt Surveillance - based on Regulation 2073 - Official sampling - Objective d) 1:2003 Coagul sampling ase-positives staphylococci Cheeses made from goats' milk - Processing plant - Bulgaria - food sample single 1 Gram N_A Not Available 5 0 Staphylococcus aureus 0 - milk - Surveillance - HACCP and own check - Objective sampling (food/fee d) Cheeses, made from mixed milk from cows, sheep and/or goats - single 1 Gram N_A Not Available 3 0 Staphylococcus aureus 0 Processing plant - Bulgaria - food sample - milk - Surveillance - HACCP (food/fee and own check - Objective sampling d) Cheeses, made from mixed milk from cows, sheep and/or goats - soft and single 1 Gram N_A EN ISO 6888- 5 0 Staphylococcus 0 semi-soft - made from pasteurised milk - Border Control Posts - Serbia - (food/fee 1:1999/Amdt food sample - Surveillance - Official sampling - Objective sampling d) 1:2003 Coagulase- positives staphylococci Molluscan shellfish - cooked - frozen - Processing plant - Bulgaria - food single 1 Gram N_A Not Available 43 0 Staphylococcus aureus 0 sample - Surveillance - HACCP and own check - Objective sampling (food/fee d) Sauce and dressings - mayonnaise - Processing plant - Bulgaria - food single 1 Gram N_A Not Available 18 0 Staphylococcus aureus 0 sample - Surveillance - HACCP and own check - Objective sampling (food/fee d)

Bulgaria - 2019 78 Table Trichinella:TRICHINELLA in animal

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Not Available Pigs - breeding animals - not raised under controlled housing conditions - sows - Slaughterhouse - Bulgaria - N_A Not Available animal 86 0 Trichinella 0 animal sample - organ/tissue - Surveillance - Official sampling - Census Pigs - fattening pigs - not raised under controlled housing conditions - Farm - Bulgaria - animal sample - N_A Magnetic stirrer animal 42 0 Trichinella 0 organ/tissue - Surveillance - HACCP and own check - Census method for pooled sample digestion Pigs - fattening pigs - not raised under controlled housing conditions - for own consumption - Farm - Bulgaria N_A Not Available animal 66 0 Trichinella 0 - animal sample - organ/tissue - Surveillance - Official sampling - Census Pigs - fattening pigs - not raised under controlled housing conditions - for own consumption - Natural habitat - N_A Not Available animal 32 1 Trichinella 1 Bulgaria - animal sample - organ/tissue - Surveillance - HACCP and own check - Census Pigs - fattening pigs - raised under controlled housing conditions - Natural habitat - Bulgaria - animal sample - N_A Not Available animal 303 0 Trichinella 0 organ/tissue - Surveillance - Official sampling - Census Pigs - fattening pigs - raised under controlled housing conditions - Processing plant - Bulgaria - animal N_A Magnetic stirrer animal 6046 0 Trichinella 0 sample - organ/tissue - Surveillance - Official sampling - Census method for pooled sample digestion Pigs - fattening pigs - raised under controlled housing conditions - Slaughterhouse - Bulgaria - animal sample N_A Not Available animal 27651 0 Trichinella 0 - organ/tissue - Surveillance - Official sampling - Census Pigs - fattening pigs - raised under controlled housing conditions - Slaughterhouse - Bulgaria - animal sample N_A Magnetic stirrer animal 33881 0 Trichinella 0 - organ/tissue - Surveillance - Official sampling - Census method for pooled sample digestion Pigs - fattening pigs - raised under controlled housing conditions - Slaughterhouse - Bulgaria - animal sample N_A Artificial animal 14821 0 Trichinella 0 - organ/tissue - Surveillance - Official sampling - Census digestion test for in vivo detection of Trichinella spp. larvae in meat samples, PrioCheck Trichinella AAD Kit Pigs - mixed herds - not raised under controlled housing conditions - Slaughterhouse - Bulgaria - animal N_A Artificial animal 582 0 Trichinella 0 sample - organ/tissue - Surveillance - Official sampling - Census digestion test for in vivo detection of Trichinella spp. larvae in meat samples, PrioCheck Trichinella AAD Kit Solipeds, domestic - horses - Farm - Bulgaria - animal sample - organ/tissue - Surveillance - Official sampling N_A Not Available animal 1 0 Trichinella 0 - Census Solipeds, domestic - horses - Processing plant - Bulgaria - animal sample - organ/tissue - Surveillance - N_A Magnetic stirrer animal 20 0 Trichinella 0 Official sampling - Census method for pooled sample digestion Wild boars - wild - Game handling establishment - Bulgaria - animal sample - organ/tissue - Surveillance - N_A Magnetic stirrer animal 794 1 Trichinella 1 Official sampling - Census method for pooled sample 798 3 Trichinella spiralis 3 digestion Wild boars - wild - Game handling establishment - Bulgaria - animal sample - organ/tissue - Surveillance - N_A Artificial animal 2911 8 Trichinella 8 Official sampling - Census digestion test for in vivo detection of Trichinella spp. larvae in meat samples, PrioCheck Trichinella AAD Kit Wild boars - wild - Hunting - Bulgaria - animal sample - organ/tissue - Surveillance - HACCP and own check - N_A Not Available animal 1127 2 Trichinella 2 Census

Bulgaria - 2019 79 Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampler - Sampling Sampling units units Metrics N of units Area of Sampling strategy Sampling Details Method unit tested positive Zoonoses positive Not Available Wild boars - wild - Hunting - Bulgaria - animal sample - organ/tissue - Surveillance - HACCP and own check - N_A Magnetic stirrer animal 1536 16 Trichinella 16 Census method for pooled sample digestion Wild boars - wild - Hunting - Bulgaria - animal sample - organ/tissue - Surveillance - Official sampling - N_A Not Available animal 812 6 Trichinella 6 Census 3329 14 Trichinella 14

Bulgaria - 2019 80 Table Trichinella:TRICHINELLA in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Meat from horse - meat products - fresh raw sausages - Unspecified - single 1 Gram N_A Magnetic stirrer 2 0 Trichinella 0 Bulgaria - food sample - Unspecified - Private sampling - Census (food/fee method for d) pooled sample digestion Meat from wild boar - meat products - fresh raw sausages - Unspecified - single 1 Gram N_A Magnetic stirrer 1 1 Trichinella spiralis 1 Bulgaria - food sample - Unspecified - Private sampling - Census (food/fee method for d) pooled sample digestion

Bulgaria - 2019 81 Table Vibrio:VIBRIO in food

Total Total Matrix - Sampling stage - Sampling origin - Sample type - Sampling context - Sampling Sample Sample units units Metrics N of units Area of Sampling Sampler - Sampling strategy unit weight weight unit Sampling Details Method tested positive Zoonoses positive Not Available Molluscan shellfish - raw - Farm - Bulgaria - food sample - Surveillance - batch 25 Gram N_A Detection 1 0 Vibrio 0 HACCP and own check - Objective sampling (food/fee method d) presence in x g

Bulgaria - 2019 82 FOODBORNE OUTBREAKS TABLES

Foodborne Outbreaks: summarized data when numbers referring to cases, hospitalized people and deaths are reported as unknown, they will be not included in the sum calculation

Outbreak strenght Strong Weak N N Metrics Causative agent Food vehicle N outbreaks N human cases hospitalized N deaths N outbreaks N human cases hospitalized N deaths Escherichia coli Unknown 1 6 0 0 Staphylococcus aureus Unknown 1 4 1 0 Meat and meat products 1 19 19 0 Trichinella, unspecified sp. Pig meat and products thereof 2 27 1 0 Unknown Broiler meat (Gallus gallus) and products thereof 1 5 0 0 Mixed food 1 4 2 0 Unknown 9 85 27 0

Bulgaria - 2019 83 Strong Foodborne Outbreaks: detailed data

M e tr i N Causative Other Causative FBO nat. More food vehicle Nature of Place of origin Origin of food Contributory c N human N N CAUSATIVEOUTBREAKCAUSATIVEREPORTINGagent AGENT STRENGTH AGENT YEAR GROUPH GROUPAG VT Agent code Outbreak type Food vehicle info evidence Setting of problem vehicle factors Comment s outbreaks cases hosp. deaths N YS N2Staphylococc unk Not Not Not Available 11 Not Available Unknown N_A Detection of Not School or Not Not Available N_A o et o0us aureus Availabl Availabl causative Available kindergarten Available t sr t 1 e e agent in food A o A9 chain or its v n v environment - 1 4 1 0 a g a Detection of i i indistinguisha l l ble causative a a agent in b b humans l l 4 Not Available Meat and meat N_A Detection of Not Household Not Not Available N_A e e products causative Available Available agent in food chain or its environment - 1 19 19 0 Detection of indistinguisha ble causative agent in humans Trichinella, unk Not Not Not Available 5 Not Available Pig meat and N_A Detection of Not Household Not Not Available N_A unspecified Availabl Availabl products thereof causative Available Available sp. e e agent in food vehicle or its component - 2 27 1 0 Detection of indistinguisha ble causative agent in humans

Bulgaria - 2019 84 Weak Foodborne Outbreaks: detailed data

M e tr i N Causative Other Causative FBO nat. More food vehicle Nature of Place of origin Origin of food Contributory c N human N N CAUSATIVECAUSATIVEOUTBREAKREPORTINGagent AGENT AGENT STRENGTH YEAR GROUP GROUPH AG VT Agent code Outbreak type Food vehicle info evidence Setting of problem vehicle factors Comment s outbreaks cases hosp. deaths NNNW2Escherichia un Not Not Not Available 10 Not Available Unknown N_A Detection Not Available School or Not Available Not Available N_A o ooe0coli k Available Available of kindergarten t t a1 causative AA k9 agent in v v food chain a a or its i i environme l l nt - 1 6 0 0 a a Detection b b of l l indistingui e e shable causative agent in humans Unknown un Not Not Not Available 1 Not Available Broiler meat N_A Descriptiv Not Available School or Not Available Not Available N_A k Available Available (Gallus gallus) and e kindergarten products thereof environme 1 5 0 0 ntal evidence 2 Not Available Mixed food N_A Descriptiv Not Available Household Not Available Not Available N_A e environme 1 4 2 0 ntal evidence 3 Not Available Unknown N_A Analytical Not Available Others Not Available Not Available N_A epidemiol 1 7 7 0 ogical evidence 6 Not Available Unknown N_A Analytical Not Available Camp or Not Available Not Available N_A epidemiol picnic 1 15 0 0 ogical evidence 7 Not Available Unknown N_A Analytical Not Available Restaurant or Not Available Not Available N_A epidemiol Cafe or Pub ogical or Bar or 1 15 0 0 evidence Hotel or Catering service 8 Not Available Unknown N_A Analytical Not Available School or Not Available Not Available N_A epidemiol kindergarten 5 37 15 0 ogical evidence 9 Not Available Unknown N_A Analytical Not Available Household Not Available Not Available N_A epidemiol 1 11 5 0 ogical evidence

Bulgaria - 2019 85 ANTIMICROBIAL RESISTANCE TABLES FOR CAMPYLOBACTER

Bulgaria - 2019 86 ANTIMICROBIAL RESISTANCE TABLES FOR SALMONELLA

Table Antimicrobial susceptibility testing of Salmonella Hiduddify in Meat from pig - fresh - chilled

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring - active

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: OTHER AMR MON

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.5 2 16 0.064 2 2 0.125 16 256 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 1 1 1 1 1 1 1 1 1 1 1 1 1 1 N of resistant

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 1 <=4 1 <=8 1 128 1

Bulgaria - 2019 87 Table Antimicrobial susceptibility testing of Salmonella Infantis in Meat from pig - fresh

Sampling Stage: Slaughterhouse Sampling Type: food sample - meat Sampling Context: Monitoring

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: OTHER AMR MON

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.5 2 16 0.064 2 2 0.125 16 256 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 1 1 1 1 1 1 1 1 1 1 1 1 1 1 N of resistant

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 1 <=4 1 <=8 1 128 1

Bulgaria - 2019 88 Table Antimicrobial susceptibility testing of Salmonella Rissen in Meat from pig - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring - active

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: OTHER AMR MON

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.5 2 16 0.064 2 2 0.125 16 256 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 1 1 1 1 1 1 1 1 1 1 1 1 1 1 N of resistant

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 1 1 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=4 1 4 1 <=8 1 64 1 1024 1

Bulgaria - 2019 89 Table Antimicrobial susceptibility testing of Salmonella Senftenberg in Meat from pig - carcase

Sampling Stage: Slaughterhouse Sampling Type: food sample - carcase swabs Sampling Context: Monitoring

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: AMR MON

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.5 2 16 0.064 2 2 0.125 16 256 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 1 1 1 1 1 1 1 1 1 1 1 1 1 1 N of resistant

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 1 <=2 1 1 <=4 1 <=8 1 128 1

Bulgaria - 2019 90 Table Antimicrobial susceptibility testing of Salmonella Typhimurium in Meat from pig - carcase

Sampling Stage: Slaughterhouse Sampling Type: food sample - carcase swabs Sampling Context: Monitoring

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: AMR MON

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.5 2 16 0.064 2 2 0.125 16 256 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 3 3 3 3 3 3 3 3 3 3 3 3 3 3 N of resistant

Metric MIC isolates 0 0 0 0 0 0 0 0 0 0 0 0 0 0 s N <=0.015 3 <=0.03 3 <=0.25 3 3 3 <=0.5 3 3 <=1 2 3 <=2 3 2 2 1 <=4 3 4 1 <=8 3 128 3

Bulgaria - 2019 91 Table Antimicrobial susceptibility testing of Salmonella Typhimurium, monophasic in Meat from pig - meat preparation

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring - active

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: OTHER AMR MON

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.5 2 16 0.064 2 2 0.125 16 256 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 1 1 1 1 1 1 1 1 1 1 1 1 1 1 N of resistant

Metric MIC isolates 1 0 0 0 0 0 0 0 0 0 1 1 0 0 s N <=0.015 1 <=0.03 1 <=0.25 1 1 1 <=0.5 1 1 <=1 1 <=4 1 4 1 <=8 1 64 1 >64 1 >1024 1

Bulgaria - 2019 92 ANTIMICROBIAL RESISTANCE TABLES FOR INDICATOR ESCHERICHIA COLI

Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from bovine animals - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON pnl2

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test Ceftazidime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ECOFF 0.125 0.25 0.25 8 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.064 0.25 0.064 0.5 0.25 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 128 128 2 16 16 128 N of tested isolates 36 36 36 36 36 36 36 36 36 36 N of resistant

Metric MIC isolates 35 36 1 1 33 1 0 0 0 0 s N <=0.015 32 <=0.03 36 0.03 4 <=0.064 32 <=0.12 31 33 0.12 1 3 0.25 4 3 0.5 3 1 2 7 1 2 6 14 10 5 4 12 1 1 19 4 21 Bulgaria - 2019 93 AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test Ceftazidime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ECOFF 0.125 0.25 0.25 8 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.064 0.25 0.064 0.5 0.25 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 128 128 2 16 16 128 N of tested isolates 36 36 36 36 36 36 36 36 36 36 N of resistant

Metric MIC isolates 35 36 1 1 33 1 0 0 0 0 s N 8 8 1 2 8 1 8 16 5 5 3 1 32 2 7 1 1 64 8 >64 14

Bulgaria - 2019 94 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from bovine animals - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 36 36 36 36 36 36 36 36 36 36 36 36 36 36 N of resistant

Metric MIC isolates 36 2 36 33 8 24 0 7 0 16 32 28 0 15 s N <=0.015 11 <=0.03 36 0.03 1 0.12 1 <=0.25 36 12 0.25 7 <=0.5 3 24 0.5 1 6 <=1 35 1 10 5 <=2 13 7 2 7 3 1 3 <=4 15 4 16 1 6 1 >4 35 <=8 28 8 4 6 3 1 5 1 >8 4 8 16 1 1 32 4 1 2 4 >32 1 15

Bulgaria - 2019 95 AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 36 36 36 36 36 36 36 36 36 36 36 36 36 36 N of resistant

Metric MIC isolates 36 2 36 33 8 24 0 7 0 16 32 28 0 15 s N 64 2 11 >64 36 2 13 128 4 >128 4 15 512 1 1024 1 >1024 30

Bulgaria - 2019 96 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Pigs - fattening pigs

Sampling Stage: Slaughterhouse Sampling Type: animal sample - caecum Sampling Context: Monitoring

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: AMR MON

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 104 104 104 104 104 104 104 104 104 104 104 104 104 104 N of resistant

Metric MIC isolates 62 1 0 0 38 17 0 1 0 11 69 78 0 38 s N <=0.015 79 <=0.03 104 0.064 8 0.12 6 <=0.25 104 99 61 0.25 7 <=0.5 104 94 0.5 2 4 3 <=1 12 104 1 8 1 2 <=2 27 26 2 24 1 <=4 86 4 6 63 <=8 65 8 11 1 7 >8 1 16 2 1 10 1 32 8 3 8 2 >32 1 38 64 4 3 17 32

Bulgaria - 2019 97 AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 104 104 104 104 104 104 104 104 104 104 104 104 104 104 N of resistant

Metric MIC isolates 62 1 0 0 38 17 0 1 0 11 69 78 0 38 s N >64 58 1 43 128 11 3 >128 16 5 256 3 512 2 1024 6 >1024 58

Bulgaria - 2019 98 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Pigs - fattening pigs

Sampling Stage: Slaughterhouse Sampling Type: animal sample - caecum Sampling Context: Monitoring

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON pnl2

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test Ceftazidime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ECOFF 0.125 0.25 0.25 8 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.064 0.25 0.064 0.5 0.25 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 128 128 2 16 16 128 N of tested isolates 81 81 81 81 81 81 81 81 81 81 N of resistant

Metric MIC isolates 80 81 2 1 73 2 0 0 0 1 s N <=0.015 76 <=0.03 79 0.03 5 <=0.064 1 69 0.064 2 <=0.12 64 75 0.12 8 <=0.25 1 0.25 2 14 2 0.5 2 7 1 4 1 4 2 2 17 1 2 16 1 36 18 19 4 29 32 21 42 8 24 3 12 12 19 Bulgaria - 2019 99 AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test Ceftazidime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ECOFF 0.125 0.25 0.25 8 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.064 0.25 0.064 0.5 0.25 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 128 128 2 16 16 128 N of tested isolates 81 81 81 81 81 81 81 81 81 81 N of resistant

Metric MIC isolates 80 81 2 1 73 2 0 0 0 1 s N 16 3 15 4 1 32 2 18 64 27 1 >64 15 1 >128 1

Bulgaria - 2019 100 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Pigs - fattening pigs

Sampling Stage: Slaughterhouse Sampling Type: animal sample - caecum Sampling Context: Monitoring

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 81 81 81 81 81 81 81 81 81 81 81 81 81 81 N of resistant

Metric MIC isolates 81 6 81 73 25 34 4 15 0 28 62 63 0 31 s N <=0.015 39 <=0.03 80 0.03 5 0.064 3 0.12 1 1 <=0.25 80 40 0.25 10 <=0.5 8 60 0.5 4 1 7 <=1 71 1 1 23 6 3 <=2 27 17 2 1 15 1 6 <=4 47 4 39 21 8 4 1 >4 79 <=8 55 8 6 11 2 5 6 >8 3 8 16 3 1 4 8 32 1 4 2 5 2 9 9

Bulgaria - 2019 101 AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 81 81 81 81 81 81 81 81 81 81 81 81 81 81 N of resistant

Metric MIC isolates 81 6 81 73 25 34 4 15 0 28 62 63 0 31 s N >32 1 31 64 1 1 3 2 35 >64 79 1 19 128 16 1 5 >128 7 22 512 1 1024 2 >1024 54

Bulgaria - 2019 102 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from pig - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON pnl2

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test Ceftazidime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ECOFF 0.125 0.25 0.25 8 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.064 0.25 0.064 0.5 0.25 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 128 128 2 16 16 128 N of tested isolates 29 29 29 29 29 29 29 29 29 29 N of resistant

Metric MIC isolates 29 29 1 0 25 0 0 0 0 0 s N <=0.015 24 <=0.03 29 0.03 3 <=0.064 27 0.064 2 <=0.12 25 29 0.12 1 <=0.25 1 0.25 1 3 0.5 1 3 1 1 2 1 7 2 5 11 7 2 4 9 1 18 7 24 8 8 1 3 2 Bulgaria - 2019 103 AM substance Cefepime Cefotaxim Cefotaxime + Clavulanic acid Cefoxitin Ceftazidim Ceftazidime + Clavulanic acid Ertapenem Imipenem Meropenem Temocillin Cefotaxime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test Ceftazidime Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available Not Available synergy test ECOFF 0.125 0.25 0.25 8 0.5 0.5 0.06 0.5 0.125 32 Lowest limit 0.064 0.25 0.064 0.5 0.25 0.12 0.015 0.12 0.03 0.5 Highest limit 32 64 64 64 128 128 2 16 16 128 N of tested isolates 29 29 29 29 29 29 29 29 29 29 N of resistant

Metric MIC isolates 29 29 1 0 25 0 0 0 0 0 s N 16 2 5 1 1 32 2 4 64 8 >64 9

Bulgaria - 2019 104 Table Antimicrobial susceptibility testing of Escherichia coli, non-pathogenic, unspecified in Meat from pig - fresh

Sampling Stage: Retail Sampling Type: food sample - meat Sampling Context: Monitoring

Sampler: Official sampling Sampling Strategy: Objective sampling Programme Code: ESBL MON

Analytical Method:

Country of Origin: Bulgaria

Sampling Details:

AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 29 29 29 29 29 29 29 29 29 29 29 29 29 29 N of resistant

Metric MIC isolates 29 2 29 25 14 22 0 4 0 12 26 27 0 9 s N <=0.015 6 <=0.03 29 0.03 1 <=0.25 29 16 0.25 11 <=0.5 4 22 0.5 2 <=1 26 1 1 8 2 1 2 <=2 6 2 2 6 1 3 2 <=4 9 4 16 1 7 3 >4 27 <=8 15 8 3 3 4 1 8 >8 1 1 16 2 1 2 32 3 2 1 2 6 >32 9 64 1 1 1 11

Bulgaria - 2019 105 AM substance Ampicillin Azithromycin Cefotaxim Ceftazidim Chloramphenicol Ciprofloxacin Colistin Gentamicin Meropenem Nalidixic acid Sulfamethoxazole Tetracycline Tigecycline Trimethoprim ECOFF 8 16 0.25 0.5 16 0.064 2 2 0.125 16 64 8 1 2 Lowest limit 1 2 0.25 0.5 8 0.015 1 0.5 0.03 4 8 2 0.25 0.25 Highest limit 64 64 4 8 128 8 16 32 16 128 1024 64 8 32 N of tested isolates 29 29 29 29 29 29 29 29 29 29 29 29 29 29 N of resistant

Metric MIC isolates 29 2 29 25 14 22 0 4 0 12 26 27 0 9 s N >64 29 1 8 128 4 2 2 >128 6 9 >1024 24

Bulgaria - 2019 106 OTHER ANTIMICROBIAL RESISTANCE TABLES

Bulgaria - 2019 107 Specific monitoring of ESBL-/AmpC-/carbapenemase-producing bacteria and specific monitoring of carbapenemase-producing bacteria, in the absence of isolate detected

Total Total Programme Matrix Zoonotic Agent Sampling Sampling Sampling Sampling Metrics Units Units Code Detailed Detailed Strategy Stage Details Context Sampler Sample Type Sampling Unit Type Sample Origin Comment Tested Positive CARBA Meat Escherichia Objective Retail CARB/O Monitorin Official food sample - batch (food/feed) Bulgaria N_A MON from coli, non- sampling XA, g samplin meat bovine pathogenic, chromID g animals - unspecified CARBA 150 0 fresh - SMART, chilled Biomerie ux Meat Escherichia Objective Retail CARB/O Monitorin Official food sample - batch (food/feed) Bulgaria N_A from pig - coli, non- sampling XA, g samplin meat fresh - pathogenic, chromID g chilled unspecified CARBA 150 0 SMART, Biomerie ux Pigs - Escherichia Objective Slaughte CARB/O Monitorin Official animal slaughter animal Bulgaria N_A fattening coli, non- sampling rhouse XA, g samplin sample - batch pigs pathogenic, chromID g caecum unspecified CARBA 153 0 SMART, Biomerie ux

Bulgaria - 2019 108 Specific monitoring of ESBL-/AmpC-/carbapenemase-producing bacteria and specific monitoring of carbapenemase-producing bacteria, in the absence of isolate detected

Bulgaria - 2019 109 Latest Transmission set

Last submitted Metrics dataset Table Name transmission date Antimicrobial Resistance 06-Aug-2020 Esbl 06-Aug-2020 Animal Population 06-Aug-2020 Disease Status 06-Aug-2020 Food Borne Outbreaks 06-Aug-2020 Prevalence 06-Aug-2020

Bulgaria - 2019 110

Institutions and Laboratories involved in zoonoses monitoring and reporting

Bulgarian Food Safety Agency (BFSA) is the competent authority (CA) responsible for food control and food safety in Bulgaria. Since its establishment in 2011 BFSA performs the official control in the area of animal health and animal welfare; phytosanitary; plant protection products; veterinary medicinal products; animal by products not intended for human consumption; feed control; food contact materials, raw materials and food except bottled natural mineral, spring and table waters; assuring the conformity of the quality of fresh fruit and vegetables with European Union marketing standards.

BFSA assesses the compliance of food quality characteristics with the requirements of national standards, standards developed by branch organizations and approved by the competent authority and technological documentation. The remit of competences of the BFSA cover also laboratory-diagnostic and research activities and other activities in the frame of the Law on the Bulgarian Food Safety Agency.

BFSA includes also specialized structures as the National Diagnostic and Research Veterinary Medical Institute (NDRVMI - http://www.ndnivmi.bg/) with its National reference laboratories (NRL - http://www.babh.government.bg/bg/Page/315/index/315/%D0%9D%D0%B0%D1%86%D0%B8%D0% BE%D0%BD%D0%B0%D0%BB%D0%BD%D0%B8), performing laboratory diagnosis and research activities in the area of animal health and food safety which annually provide data for zoonotic agents in Bulgaria.

BFSA includes also 28 Regional Food Safety Directorates (RFSD - http://www.babh.government.bg/bg/Page/contacts-regions/index/contacts- regions/%D0%9E%D0%B1%D0%BB%D0%B0%D1%81%D1%82%D0%BD%D0%B8%20%D0%B4% EF%BF%BD), one in each administrative division in Bulgaria, responsible for the official control at regional level.

Within 8 of 28 RFSDs there are situated regional testing laboratories which annually provide data for zoonotic agents in Bulgaria as follows:

1. Testing Laboratory "Laboratory activities", RFSD Varna; 2. Testing Laboratory "Laboratory activities", RFSD Sliven; 3. Testing Laboratory "Laboratory activities", RFSD Shumen; 4. Testing Laboratory "Laboratory activities", RFSD Vratza; 5. Testing Laboratory "Laboratory activities", RFSD Plovdiv; 6. Testing Laboratory "Laboratory activities", RFSD Haskovo; 7. Testing Laboratory "Laboratory activities", RFSD Gabrovo; 8. Testing Laboratory "Laboratory activities", RFSD Blagoevgrad.

More information is available on the BFSA website on the following link: http://www.babh.government.bg/en/Page/Laboratories/index/Laboratories/Laboratories

List of laboratories (state and private) engaged in the implementation of the national programme for prevention, surveillance, control and eradication of animal diseases including zoonoses for the period 2019 – 2021 is published on the link below:

http://bfsa.bg/userfiles/files/ZJ/%20%D1%81%20%D0%B0%D0%BA%D1%80%D0%B5%D0%B4%D0 %B8%D1%82%D0%B0%D1%86%D0%B8%D0%B8%20%D0%B2%20%D1%81%D0%B8%D1%81% D1%82%D0%B5%D0%BC%D0%B0%D1%82%D0%B0%20%D0%BD%D0%B0%20%D0%91%D0%9 0%D0%91%D0%A5%20050602019.pdf

1 Bulgaria

There are also private laboratories in Bulgaria, designated by BFSA to perform activities for the purpose of the official control and involved in the diagnosis and providing data to EFSA for animal diseases and food pathogens – more information available at the following link: http://www.babh.government.bg/bg/Object/site_register/view/10/%D0%92%D0%B5%D1%82%D0% B5%D1%80%D0%B8%D0%BD%D0%B0%D1%80%D0%BD

The laboratories are accredited according under БДС EN ISO/IEC 17025:2006 and carry out tests in the area of food and feed safety and animal health. The RTL carry out tests of samples taken from more than one region in the country according to a distribution schedule. Their work is methodologically co- ordinated by the NRLs in the frame of NDRVMI.

BFSA, as a CA in Bulgaria, maintains national identification and registration system (VETIS), which contains data for livestock subject to identification (large, small ruminants, equids and pigs). It maintains information and registers regarding the livestock holdings (sites) on the Bulgarian territory as well as other information on the livestock population provided by the regional food safety directorates.

Information about the organizational structure of BFSA is available on the following link: http://www.babh.government.bg/en/Page/structure/index/structure/Organisation%20Structu

Short description of the institutions and laboratories involved in data collection and reporting

Animal population

1. Sources of information and the date(s) (months, years) the information relates to(a)

Source of information for animal population data (large ruminants – bovines and buffaloes; small ruminants – sheep and goats and pigs) for 2019 is BFSA national identification and registration system (VETIS) as per 31.12.2019.

The data for equids is provided by the Regional food safety directorates (RFSD) by regions as per 30.08.2019.

Source of information for poultry population (Gallus Gallus – laying hens and pullets and broilers), on the number of turkeys, ducks and geese is Agrostatistics within the Ministry of Agriculture, Food and Forestry of Bulgaria - http://www.mzh.government.bg/bg/statistika-i-analizi/izsledvane-zhivotnovdstvo/tseli/ as per 01.01.2019.

The data regarding the number of poultry holdings in Bulgaria and number of flocks of certain poultry categories is provided by BFSA/RFSD.

The information for wild boars population in Bulgaria for 2019 is derived from the Ministry of Agriculture, Food and Forestry of Bulgaria.

The information source for fish farms (carp; trout, silver carp holdings) is BFSA/RFSD as per 31.12.2019.

The information source for the number of bee colonies is BFSA/RFSD, as per 18.10.2019 г.

2. Definitions used for different types of animals, herds, flocks and holdings as well as the production types covered

Bovines:

2 Bulgaria

The population data includes (1) number of animal by categories (calves under 12 months of age, including 2 sub categories: calves under 3 months and calves between 3 and 12 months; young bovines between 12 - 24 months and adult animals – above 24 months); (2) data for animal holdings. Buffaloes: Data on number of animals and animal holdings.

Small ruminants (sheep and goats): The population data includes (1) number of animal by categories (animals under 12 months. female animals above 12 months for milk, meat and other animals; (2) data for animal holdings (more often practice is to have mixed holdings for sheep and goats).

Equids: Information is provided on the total number of animals in the country. It includes horses, donkeys and mules, including breeding animals.

NB! The entire population of equids in Bulgaria is not fully identified until the end of 2019. Part of the data is derived and supplemented by the animals’ registers in the RFSD in Bulgaria and there is no complete records for the numbers of equids in the national identification and registration system (VETIS) as per the end of 2019.

Pigs: Information is provided on the total number of animals in the country by categories (piglets under 2 months; sows – female animals above 11 months and “Unspecified”- pigs other than the above mentioned categories as well as data on the total number of the pig farms in Bulgaria. It is included also information (population data and number of holdings) on the so called East Balkan Pigs – local type of pigs, inhabited certain regions of Bulgaria.

Poultry - Gallus Gallus (fowl): Information is provided on the total number of (1) poultry from the following categories: laying hens and pullets (holdings with more than 100 birds kept in) and broilers. The number of birds in the backyards are not included and (2) number of holdings for laying hens and broilers.

The information is provided on the number of turkeys, ducks and geese.

Information is provided on the number of fish farms (carp; trout, silver carp holdings) in the country.

Information is provided on the number of bee colonies and apiarys.

Information is provided on the total number of wild boars in Bulgaria.

3. National changes of the numbers of susceptible population and trends

Changes in the population size and the number of holdings in 2019 are observed mainly for ruminants, and pigs compared to 2018.

For a consecutive year there is a certain reduction in the number of bovine animal holdings in Bulgaria. The main reason for this is a reduction in the number of livestock holdings (“backyards”) as well as lower number of animal owners (farmers) in 2019. Another reason is the multi - stage update of the central database (the national identification and registration system, VETIS) carried out in 2019.

Same trend is observed for small ruminants as well.

There is a decrease in the number of domestic pigs population as well as the number of pig holdings in Bulgaria in 2019, due to the implemented control measures for ASF including depopulation of a number of pig farms.

3 Bulgaria

There was a slight decrease in the equidae population in Bulgaria in 2019. These animals are mainly kept in small private farms (up to 3 adult animals and very often one to two animals). It is common practice horses and donkeys in Bulgaria to be kept in small farms and backyard by private owners for their own needs (work). The tendency for decreasing of the number of equids is due to economic reasons (it is not economically reliable), and partly due to the reduction of the number of farmers/owners.

Decrease in the number of bee colonies is observed in 2019.

4. Geographical distribution and size distribution of the herds, flocks and holdings(b)

n/a 5. Additional information

More information and national registers of animal population and holdings are available on the website of the BFSA at the following link: http://bfsa.bg/bg/Object/site_register/view/5/%D0%97%D0%B4%D1%80%D0%B0%D0%B2%D0%B5 %D0%BE%D0%BF%D0%B0%D0%B2 And on the website fo the Ministry of Agriculture, Food and Forestry - https://www.mzh.government.bg/bg/statistika-i-analizi/izsledvane-zhivotnovdstvo/danni/

(a): National identification and registration system(s), source of reported statistics (Eurostat, others) (b): Link to website with density maps if available, tables with number of herds and flocks according to geographical area

4 Bulgaria

General evaluation*: Brucellosis 1. History of the disease and/or infection in the country(a)

Bulgaria developed and implement а 3 years National Program for the Prevention, Surveillance, Control and Eradication of Animal and Zoonotic Diseases for the period 2019-2021, in accordance with the Law on the Veterinary Activities.

Bovine Brucellosis: There is no data for infected with bovine brucellosis herd on the whole territory of Bulgaria for the period 2011 – 2019. The aim of the national programme for disease prevention, surveillance, control and eradication is to prove disease absence on the country territory and to achieve bovine brucellosis free status in Bulgaria, as required by EU legislation by the end of 2021.

Ovine & Caprine brucellosis: (Brucella melitensis) The aim of the national programme for prevention, surveillance, control and eradication of brucellosis in small ruminants is timely detection of the farms affected by ovine/caprine brucellosis and timely implementation of effective measures for disease eradication in order to maintain a status of Bulgaria as a country officially free of brucellosis in small ruminants by the end of 2019.

Disease history: In 2005 г. A case of Brucella melitensis is reported in humans, who worked in Greece. For the period 2005- 2008 г., 120 human cases are registered in 12 districts in Bulgaria (most cases in Haskovo and Sliven districts) The epidemiological investigation proved that some of the cases are imported (from Greece, Cyprus, Italy and Tansania) and other cases are local – owners who consumed dairy products without efficient heat treatment)

In June 2006 the first cases of brucellosis in sheep and goats are confirmed in Smolyan district Until the end of 2016 the disease is reported in 16 villages in 4 different districts in Bulgaria: Smolyan, Yambol, Haskovo and Stara zagora districts. In total 496 goats, 117 sheep and 7 bovines are culled and destroyed as a measure for disease control. (Fig.1).

Fig.1 – Location of the aaffected villages and number of infected animals (Brucella melitensis) by regions in Bulgaria for the period 2006 – 2008 г.

5 Bulgaria

In 2015 (on 27 July 2015) new cases of brucellosis were confirmed in small ruminants in Kyustendil district, Rila town. Until 10-th of October 2015 in totally 636 goats were tested, 106 (16,7 %). of the with positive result. In addition 20 dogs were tested, 8 (40%) of them with positive result. All tested sheep (n=360), horses (n- 6) and pigs (n= 27) are negative. The total number of affected farms in Rila town is 22. (Fig. 2).

Fig. 2: Geographical distribution of brucellosis in small ruminants in Bulgaria in 2015

In case of high percentage of positive animal, than the entire herd was destroyed (according to the provisions of Directive 91/68/ЕИО). Until 10.10.2015, in totally 229 goats, 14 sheep, 4 bovines and 10 dogs were destroyed in Kyistendil district, as a measure for disease control. Until 1.10.2015 51 human cases were confirmed, including 2 cases in veterinarians.

Another outbreak was confirmed in Pleven district, Rakita village (around 200 meters from Kiystendil district). From 41 goats, 25 (61%) were positive.

In both epidemics there is a clear tendency of high level of infected goats than sheep. High percent of infected dogs were also observed.

Source of infection: The epidemiological investigations leads to the following possible source of infection: I-st epidemic (2005- 2008): goats from Greece. II-nd epidemic (2015): 2 possible courses of infection: (1) Greece or (2) North Macedonia

New outbreaks of brucellosis in small ruminants were reported in 2017. In august 2017 a new case of brucellosis in goats was confirmed in the frame of the routine national disease surveillance programme. The outbreak was located in the city of Sliven. The source of infection is an infected sheep coming from Gergevets village (Sliven district). All infected animals were culled and destroyed as a measure for disease control. The epidemiological investigations leads to sheep imported from Greece. The geographical distribution of the outbreaks of brucellosis in small ruminants in 2017 are presented on Figure 3.

6 Bulgaria

Fig. 3: Geographical distribution of the outbreaks of brucellosis in small ruminants in 2017

2. Evaluation of status, trends and relevance as a source for humans

n/a 3. Any recent specific action in the Member State or suggested for the European Union(b)

n/a 4. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_4_Prilojenie_4%20Brucella%20bovis%202019-2021.pdf http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_5_Prilojenie_5%20Brucella%20melitensis%202019- 2021.pdf * For each zoonotic agent (a): Epidemiological evaluation (trends and sources) over time until recent/current situation for the different relevant matrixes (food, feed, animal). If relevant: the official “disease status” to be specified for the whole country and/or specific regions within the country (b): If applicable

7 Bulgaria

General evaluation*: Tuberculosis 1. History of the disease and/or infection in the country(a)

The first reported case of bovine tuberculosis in Bulgaria is back in 1894.

For the period 1987 - 2012 г., in totally 125 outbreaks of tuberculosis in bovines are confirmed in the districts of Shumen, Vidin, Dobrich, Veliko Tarnovo, Sofia, Silistra, Smolyan, Razgrad, Ruse, Pleven, Lovech, Kurdzhali, Pazardzik, arna, Gabrovo, Vratza, Burgas, Blagoevgrad and Haskovo. (Fig. 1)

Fig. 1: Geographical distribution of bovine tuberculosis cases (and outbreaks in brackets) in Bulgaria for the period 1983-2012 г.

For the period January 2010 г. to 31-st of march 2018 the following cases of bovine tuberculosis are reported on the Bulgarian territory (fig. 2):

- 2010 – Staliyska mahala village (Montana district); - 2011 – Sredetz (Burgas district); - 2014 - In the districts of Razgrad; Gabrovo; Kyustendil; Vidin; Pleven and Kardjali - 2016 - In Plovdiv; Shumen; Razgrad and Silistra districts; - 2017 – In Plovdiv and Stara Zagora; Dobrich, Silistra, districts; Shumen and Pazardjik districts; - 2018 – until 31-th of March 2018, outbreaks of bovine tuberculosis are confirmed in Plovdiv and Kurdjali districts.

8 Bulgaria

Fig. 2 Bovine tuberculosis outbreaks in Bulgaria in the period 2010 – 2017.

In 2012 - 2013 – There are no outbreaks of bovine tuberculosis on the Bulgarian territory. Since 2014 until 2018 г. the number of bovine tuberculosis outbreaks in Bulgaria increased.

2. Evaluation of status, trends and relevance as a source for humans

n/a 3. Any recent specific action in the Member State or suggested for the European Union(b)

n/a 4. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_20_Prilojenie_20%20Tuberculosis%20bovis%202019- 2021_12.2018.pdf * For each zoonotic agent (a): Epidemiological evaluation (trends and sources) over time until recent/current situation for the different relevant matrixes (food, feed, animal). If relevant: the official “disease status” to be specified for the whole country and/or specific regions within the country (b): If applicable

General evaluation*: Rabies 1. History of the disease and/or infection in the country(a)

Rabies is mostly distributed in the north Bulgaria territories. The total number of confirmed rabies cases from the beginning of 1994 until august 2014 is 392, 332 (85%) of them in North Bulgaria and 60 cases (15%) in the southern part of the country. This geographical distribution and the higher prevalence of rabies positive cases in North Bulgaria can be explained by the fact that there is a mountain serving as a natural geographical barrier devising the country territory into two parts (North and South). The majority of the rabies positive cases in Bulgaria are in spring, less cases are observed during the autumn and summer period. The spring pick of the disease is related to the ecological and biological characteristics of the fox population in the country the fox reproductive period in spring, while the autumn- winter cases are due to the settlement of new areal from the young foxes.

9 Bulgaria

Up to 2009 (the year when the oral vaccination of foxes in Bulgaria started) the rabies positive cases are geographically distributed in administrative districts in North Bulgaria. From total 392 cases of rabies, 73 are in domestic animals (cattle, sheep, goats and horses), 83 in dogs and cats and 238 in wild animal – foxes and jackals. The higher percent of rabies cases in domestic animals can be explained by the type of animal keeping in pastures during the most time of the year which enable contact with wild animals. The firs rabies cases in South Bulgaria are registered in 2007. In the regions of Sofia, Pernik, Kyustendil and in 2009 – Burgas district. For the period 2010-2014 г. only 10 rabies cases are registered in the districts of Vratsa, Veliko Tarnovo, Shumen, Ruse, Blagoevgrad and Kyustendil with no positive case in 2013 and 2 positive cases in the spring of 2014.

2. Evaluation of status, trends and relevance as a source for humans

n/a 3. Any recent specific action in the Member State or suggested for the European Union(b)

n/a 4. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_6_Prilojenie_6%20Programme_Rabies_2019%20- %202021.pdf * For each zoonotic agent (a): Epidemiological evaluation (trends and sources) over time until recent/current situation for the different relevant matrixes (food, feed, animal). If relevant: the official “disease status” to be specified for the whole country and/or specific regions within the country (b): If applicable

General evaluation*: Q - fever 1. History of the disease and/or infection in the country(a) Q – Fever surveillance in Bulgaria is in place from 1950, previously by performing of ramdom serological testing. Up to the end of 2004, the mean percentage of positive animals is between 5,17% to 18%. Data for the number of tested and positive animals by years for the period 2011 – 2017 is presented in table 1.

Year Number of tested animal Number (%) positive animals bovines small ruminants bovines small ruminants 2011 1199 719 88 (7,34) 82 (11,40) sheep goats sheep goats 2012 430 354 18 21 (4,88) 36 (10,17) 6 (33,3) 2013 566 115 69 28 (4,94) 9 (7,82) 18 (26,86) 2014 860 733 71 21 (2,44) 22 (3) 1 (1,41) 2015 26 3 43 0 (0) 0 (0) 1 (2,3) 2016 344 504 97 23 (6,69) 43 (8,53) 6 (6,19) 2017 463 263 202 57 (12,31) 35 (13,3) 40 (19,8)

2. Evaluation of status, trends and relevance as a source for humans

The aim of the National Program for the Prevention, Surveillance, Control and Eradication of Q – Fever in Bulgaria is to reduce the risk of human infection and to gain economic profits by minimisation of the percent of abortions in ruminants and strict implementation of the requirements of OIE for trade of live animals.

10 Bulgaria

3. Any recent specific action in the Member State or suggested for the European Union(b)

n/a 4. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_10_Prilojenie_10%20Q_fever%202019%20-%202021.pdf * For each zoonotic agent (a): Epidemiological evaluation (trends and sources) over time until recent/current situation for the different relevant matrixes (food, feed, animal). If relevant: the official “disease status” to be specified for the whole country and/or specific regions within the country (b): If applicable

General evaluation*: West Nile Fever 1. History of the disease and/or infection in the country(a)

For the first time antibodies against West Nile Fever in Bulgaria were detected in the 1960s. In 1978, the virus was found in insects by an immunofluorescence method. Since 1974 up to 1994, Western Nile Fever Abs were found in serum samples of large and small ruminants, donkeys, hens, and humans on the whole country. For the period indicated above, the causative agent has been isolated several times from rabbits, hens and mosquitoes. Studies conducted over the last five years have reveal that there is an annual virus introducon the Bulgarian territory. For the period 2010-2014, the infection was confirmed in four high risk regions in Bulgaria: Durankulak and Shabla (Dobrich), Tutrakan (Silistra) and Staro Oryahovo (Varna). The studies conducted for that period are based on a laboratory test of blood samples from sentinel animals (equidae and poultry) and include a set of serological methods: ELISA (screening test), serum neutralization test (confirmatory test) and IgM ELISA-for detection of early antibodies in equine sera). In 2015, antibodies against West Nile Fever virus in sentinel animals were detected in the four regions with proven virus circulation and in some new risk areas as well: Rousse, Vidin, Lom, Kozloduy and Svilengrad. In addition, West Nile Fever virus (WNFV) genetic lineage II is detected in samples from wild birds in Sofia. The results of serological surveys conducted in Sofia region confirm the presence of WNFV antibodies in wild birds, equidae and dogs. In 2017 four cases of West Nile Fever (line 2) were confirmed in wild birds in Bourgas District (three birds in Burgas and 1 - in Karnobat).

The geographical distribution of WNF cases in Bulgaria in the period 2010 – 2017. are presented on fig. 1

11 Bulgaria

Fig. 1 Geographical distribution of WNF cases in Bulgaria for the period 2010 – 2017. (blue triangle – sero -positives (sentinel animals); red circle – virologicaly positives (wild birds)

2. Evaluation of status, trends and relevance as a source for humans

n/a 3. Any recent specific action in the Member State or suggested for the European Union(b)

n/a 4. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_19_Prilojenie_19%20Programme%20WNF_2019%20- %202021.pdf * For each zoonotic agent (a): Epidemiological evaluation (trends and sources) over time until recent/current situation for the different relevant matrixes (food, feed, animal). If relevant: the official “disease status” to be specified for the whole country and/or specific regions within the country (b): If applicable

12 Bulgaria

General evaluation*: Salmonella in breeding flocks of Gallus Gallus 1. History of the disease and/or infection in the country(a)

National Control Programme for Salmonella in breeding flocks of Gallus Gallus in Bulgaria is in place since 2008. The total number of the breeding flocks tested and the number of positive flocks, as well as the percentage of prevalence of Salmonella Еnteritidis and Salmonella Тyphimurium for the period 2012- 2016 are shown on the table below:

total number of the year Salmonella positive flocks % prevalence tested breeding flocks

2012 208 1 0,48

2013 216 2 1,03

2014 238 2 0,84

2015 237 1 0,42

2016 220 0 0

2017 262 1 0,38

The total number of Salmonella positive the breeding flocks for the period 2012-2016 is as follows:

 2012 – 1 positive flock (S. Infantis), 2 positive flocks (S. Corvallis) and 1 positive flock (S. Lomnita);  2013 – 2 positive flocks (S. Enteritidis) and 1 positive flock (S. Hadar);  2014 – 2 positive flocks (S. Infantis), 1 positive flock (S. Corvalis and S. Leopoldville);  2015 – 2 positive flocks (S. Infantis) and 1 positive flock (S. Typhimurium);  2016 – 1 positive flock (S. Infantis), 1 positive flock (S. Senftenberg), 1 positive flock (S. Kentucky) (self-control) and 1 positive flock (S. Agona);  2017 - 1 positive flock (S. Infantis). The total number of Salmonella serovars in breeding flocks are: S. Enteritidis (2); S. Тyphimurium (1); S. Infantis (6); S. Hadar (1) and S. Virchow (0) The most frequently detected Salmonella isolate in Bulgaria is S. Infantis followed by S. Enteritidis, S. Hadar, S. Agona и S. Senftenberg.

In 2019 the following Salmonella serotypes are confirmed: S. Enteritidis (1 flock), S. Typhimurium (1 flock) и S. Infantis (2 flocks). According to the measures described in the Salmonella control programm for breeding flocks, all flocks, positive for S. Enteritisis и S. Typhimurium are culled and disinfection of the infected farms is conducted.

In the farm with S.Infantis positive flock a revision of the biosecurity plans is carried out.

2. Evaluation of status, trends and relevance as a source for humans

n/a 3. Any recent specific action in the Member State or suggested for the European Union(b)

n/a

13 Bulgaria

4. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_13_Prilojenie_13%20Salmonella%20breeders%202019- 2021_16.10.2018.pdf

* For each zoonotic agent (a): Epidemiological evaluation (trends and sources) over time until recent/current situation for the different relevant matrixes (food, feed, animal). If relevant: the official “disease status” to be specified for the whole country and/or specific regions within the country (b): If applicable

General evaluation*: Salmonella in broiler flocks of Gallus Gallus 1. History of the disease and/or infection in the country(a)

The National Control Programme for Salmonella in broiler flocks of Gallus Gallus is in place in Bulgaria since 2009. The total number of the broiler flocks tested and the number of positive flocks, as well as the percentage of prevalence of Salmonella Еnteritidis and Salmonella Тyphimurium for the period 2009-2014 are shown on the table below:

year total number of the tested Salmonella positive % prevalence broiler flocks flocks

2009 1076 3 0.37

2010 997 1 0.1

2011 513 0 0

2012 510 0 0

2013 898 1 0.11

2014 342 1 0.29

2015 382 2 0.52

2016 365 0 0

2017 2204 1 0.045

The total number of Salmonella positive broiler flocks is as follows:

 2009 – 3 positive flocks (S. Enteritidis), 1 positive flock (S. Virchov), 2 positive flocks (S. Kottbus), 1 flock (S. Agona);  2010 – 1 positive flock (S. Typhimurium), 10 positive flocks (S. Infantis), 1 flock (S. Newport), 2 flock (S. Tennessee), 1 flock (S. Mbandaka);  2011 – 3 positive flocks (S. Infantis), 4 positive flocks (S. Livingstone), 5 – S. Hadar;

14 Bulgaria

 2012 – 4 positive flocks (S. Infantis), 1 positive flock (S. Hadar), 1 positive flock (S. Livingstone), 1 positive flock (S. Kentacky) and 1 flock (S. Mbandaka);  2013 – 1 positive flock (S.Enteritidis), 9 positive flocks (S.Infantis); 1 positive flock (S.Derby), 1 positive flock (S. Paratyphi);  2014 –total number of salmonella positives both from official control and self-control are: 1 positive flock (S. Typhimurium), 8 positive flocks (S. Infantis) and 1 positive flock (S. Agona);  2015 - total number of salmonella positives both from official control and self-control are: 3 positive flocks (S. Infantis), 1 positive flock (S. Stefenberg), 1 positive flock (S. Typhimurium) and 1 positive flocks (S. Enteritidis);  2016 - 1 positive flock (S. Infantis);  2017 - 1 positive flock (S. Infantis)

The number of different Salmonella serotypes identified in broilers are presented on the diagram below:

In 2019, S. Typhimurium is confirmed in 2 broiler flocks. The positive flocks are culled and disinfection of the infected farms is conducted.

2. Evaluation of status, trends and relevance as a source for humans

n/a 3. Any recent specific action in the Member State or suggested for the European Union(b)

n/a 4. Additional information More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_14_Prilojenie_14%20Salmonella%20broilers%202019- 2021_16.10.2018.pdf

* For each zoonotic agent (a): Epidemiological evaluation (trends and sources) over time until recent/current situation for the different relevant matrixes (food, feed, animal). If relevant: the official “disease status” to be specified for the whole country and/or specific regions within the country (b): If applicable

15 Bulgaria

General evaluation*: Salmonella in laying hens 1. History of the disease and/or infection in the country(a) The National Control Programme for Salmonella in laying hens is in place in Bulgaria since 2008. The total number of the laying hens flocks tested and the number of positive flocks, as well as the percentage of prevalence of Salmonella Еnteritidis and Salmonella Тyphimurium for the period 2008- 2016 are as below:

year total number of the Salmonella positive flocks % prevalence tested laying hens flocks

2008 224 2 0.89

2009 244 2 0.82

2010 250 2 0.80

2011 228 4 1.75

2012 297 2 0.67

2013 261 0 0

2014 290 0 0

2015 243 4 1.65

2016 233 5 2.15

2017 227 15 6.61

16 Bulgaria

300 250 200 150 100 50 0 2008 2009 2010 2011 2012 2013 2014 2015 2016 Общ брой изследвани стада 224 244 250 228 297 261 290 243 233 Позитивни за Салмонела 2 2 2 4 2 0 0 4 5 % на разпространение 0,89 0,82 0,80 1,75 0,67 0,00 0,00 1,65 2,15

The total number of Salmonella positive broiler flocks is as follows:  2008– 2 positive flocks (S. Enteritidis);  2009– 2 positive flocks (S. Enteritidis), 1 positive flocks (S. Abovy), 1 positive flocks (S. Corvallis), 1 positive flocks (S. Agona), 1 positive flocks (S. Virchov);  2010 – 2 positive flocks (S. Enteritidis), 6 positive flocks (S. Infantis), 1 positive flock (S. Parkroyal);  2011 – 4 positive flocks (S. Enteritidis), 2 (S. Agona), 1 (S. Hadar), 3 (S.Kottbus), 4 (S. Bareilly) and 1 positive flock (S. Infantis);  2012 – 1 positive flock (S. Enteritidis) and 1 positive flock (S.Typhimurium);  2013 - 3 positive flocks (S. Seftenberg), 1 (S. Mbandaka), 1 (S. Infantis), 1 (S. Cottbus);  2014 - 1 positive flock – by self-control (Salmonella Isangi); 2 positive flocks (1 self control and 1 official control) for Salmonella Infantis; positive flocks (Salmonella Kotbus); 1 flock (Salmonella Derby);  2015 - 3 positive flocks (S. Еnteritidis), 1 flock (S. Тyphimurium), 1 (S. Corvalis) and 1 positive flock (S. Thompson);  2016 - 5 positive flocks (S. Enteritidis), 1 (S. Infantis); 3 (S. Agona), 1 (S. Kentucky) and 2 flocks (S. Corvalis).  2017 - 3 positive flocks (Salm. Entereditis), 3 positive flocks (Salm. Infantis), 1 (S. Agona), 3 (S. Thomposn), 2 (S. Kotbus), 3 (Salm. Mbandaka) The number of different Salmonella serotypes identified in laying hens flocks are shown in diagram below:

17 Bulgaria

50 44 45 40 35 30 25 19 20 15 10 5 2 0 S. Typhimurium S. Enteritidis Other

In 2019, S. Enteritidis is confirmed as well as other serotypes: S.Infantis(3 flocks); S. Agona (1 flock) and S. Senftenberg (1 flock).

S. Enteritidis is the most frequently isolated strain in laying hens flocks in Bulgaria

2. Evaluation of status, trends and relevance as a source for humans

n/a 3. Any recent specific action in the Member State or suggested for the European Union(b)

n/a 4. Additional information More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19- 21/3_15_Prilojenie_15%20Salmonella%20laying%20hens%202019-2021_16.10.2018.pdf

* For each zoonotic agent (a): Epidemiological evaluation (trends and sources) over time until recent/current situation for the different relevant matrixes (food, feed, animal). If relevant: the official “disease status” to be specified for the whole country and/or specific regions within the country (b): If applicable

General evaluation*: Salmonella in turkeys

18 Bulgaria

1. History of the disease and/or infection in the country(a)

A research conducted in 2007 in Bulgaria show that there are no any positive finding for Salmonella presence in turkey flocks in Bulgaria. The National Control Programme for Salmonella in turkeys is in place in Bulgaria since 2010 (approved by Commission decision 2009/883. The programme is implemented in the entire country. The total number of the tested turkey flocks and the number tested samples and positive samples for the period 2008-2016 are as below:

Година Брой стада, включени Брой взети проби Брой положителни в програмата стада

2009 4 4 0

2010 2 2 0

2011 3 4 0

2012 7 6 0

2013 8 11 0

2014 10 7 0

2015 11 4 0

2016 8 4 0

2017 3 3 0

There are no Salmonella positive turkey flocks for the above mention period.

There are no Salmonella positive results in 2019 as well.

2. Evaluation of status, trends and relevance as a source for humans n/a 3. Any recent specific action in the Member State or suggested for the European Union(b) n/a 4. Additional information More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19- 21/3_16_Prilojenie_16%20Salmonella%20tur%D0%BAey%202019-2021_16.10%202018.pdf

* For each zoonotic agent (a): Epidemiological evaluation (trends and sources) over time until recent/current situation for the different relevant matrixes (food, feed, animal). If relevant: the official “disease status” to be specified for the whole country and/or specific regions within the country (b): If applicable

19 Bulgaria

Description of Monitoring/Surveillance/Control programmes system*: Brucellosis 1. Monitoring/Surveillance/Control programmes system(a) Bovine Brucellosis:

Programme range:  single serological testing of all bovines above 24 months of age (with an exception of milk production line in the dairy farms);  laboratory testing of bulk milk samples from dairy farms from a category 10 to 49 bovines from milk production lines, min. 3 times per year;  laboratory testing – twice a year (in minimum 3 months intervals) of bovines in dairy farms with more than 50 animals;  double serological testing of all bovines with abortions (once right after the abortion and second time 15-20 days after the abortion);  bacteriological testing of all aborted foetuses or placentas or stillborn calves;  double serological testing of all bulls in the regional center for selection and reproduction;  single serological testing during the quarantine of all imported animals from outside EU (testing is paid by animals owners);  serological testing each 2 months of all animals (bovines, small ruminants, pigs, equide, dogs) in the affected villages  culling of all positively tested animals;  compensation for owners Bulk milk samples: - Herds up to 100 dairy cows - 1 bulk milk samples – 50 ml. - Herds up to 200 dairy cows - 2 bulk milk samples – 50 ml. - Herds up to 300 dairy cows - 3 bulk milk samples – 50 ml. etc. In each heard a groups from 100 animals are created and from the first group of 100 animals – the bulk milk sample is taken from the cooling facilities for that group and then from each group of 100 animals – a milk sample (10-15 ml) is taken from each individual animal in a common plate (50 ml of which than compose the bulk milk sample for that group).

More information is available on the website of the BFSA at the following link: More information on the http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_4_Prilojenie_4%20Brucella%20bovis%202019-2021.pdf

Ovine & Caprine brucellosis: (Brucella melitensis)

Programme range:  single serological testing of all small ruminants (sheep and goats) above 6 months of age in herds with less than 50 animals;  single serological testing of 25 % of all small ruminants (sheep and goats) above 6 months of age in herds with more than 50 animals;  single serological testing of all uncastrated male breeding animals (rams) of 6 months of age and above;  single serological testing during the quarantine of all imported animals from outside EU  double serological testing of all small ruminants with abortions (once right after the abortion and second time 15-20 days after the abortion);  bacteriological testing of all aborted foetuses or placentas or stillborn animals;  serological testing each 2 months of all animals (bovines, small ruminants, pigs, equine, dogs) in the affected villages

2. Measures in place(b) na

20 Bulgaria

3. Notification system in place to the national competent authority(c)

n/a 4. Results of investigations and national evaluation of the situation, the trends (d) and sources of infection(e)

n/a 5. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_4_Prilojenie_4%20Brucella%20bovis%202019-2021.pdf http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_5_Prilojenie_5%20Brucella%20melitensis%202019- 2021.pdf

* For all combinations of zoonotic agents and matrix (Food, Feed and Animals) for ‘Prevalence’ and ‘Disease Status’: one text form reported per each combination of matrix/zoonoses or zoonotic agent (a): Sampling scheme (sampling strategy, frequency of the sampling, type of specimen taken, methods of sampling (description of sampling techniques) + testing scheme (case definition, diagnostic/analytical methods used, diagnostic flow (parallel testing, serial testing) to assign and define cases. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (b): The control program/strategies in place, including vaccination if relevant. If applicable a description of how eradication measures are/were implemented, measures in case of the positive findings or single cases; any specific action decided in the Member State or suggested for the European Union as a whole on the basis of the recent/current situation, if applicable. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (c): Mandatory: Yes/No. (d): Minimum five years. (e): Relevance of the findings in animals to findings in foodstuffs and for human cases (as a source of infection).

Description of Monitoring/Surveillance/Control programmes system*: Tuberculosis 1. Monitoring/Surveillance/Control programmes system(a) Programme range:  Diagnostic tests on the spot – annual single intradermal tuberculinisation of all bovine animals above 42 days old in herd with status of officially tuberculosis free;  Diagnostic tests on the spot – differential tuberculinisation on 42-th day of the first tuberculinisation of bovine suspect animals in herd with status of officially tuberculosis free. All bovine animal with positive result on a single intradermal test are subjected to slather for diagnostic purposes;  Diagnostic tests on the spot – single intradermal tuberculinisation with bovine tuberculin – in the period between 45 and 60-th day after the differential tuberculinisation. All bovines reacted positively on the differential tuberculinisation are subjected to slather for diagnostic purposes;  In herds with status of officially infected with bovine tuberculosis – intradermal tests each 2 months counted from the date of slaughtering of the last infected animal are performed on all bovines above 42 days old. In case of negative results that the next testing have to be performed after 6 months to prove status of herds officially tuberculosis free;  Diagnostic slaughtering of all positive or suspect animals;  Laboratory testing (microbiological test and PCR) from suspect animals or animals from routine slaughter;  Laboratory testing (microbiological test and PCR) from susceptible wild animals in case of suspicion;  Diagnostic tests on the spot – single intradermal tuberculinisation of all bovine animals above 42 days old, imported from third countries (testing is paid by animals owners);  single intradermal tuberculinisation of pigs (with bovine tuberculin) in mixed herd with tuberculosis positive bovines

More information is available on the website of the BFSA at the following link:

21 Bulgaria

http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_20_Prilojenie_20%20Tuberculosis%20bovis%202019- 2021_12.2018.pdf 2. Measures in place(b)

n/a 3. Notification system in place to the national competent authority(c)

n/a 4. Results of investigations and national evaluation of the situation, the trends (d) and sources of infection(e)

n/a 5. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_20_Prilojenie_20%20Tuberculosis%20bovis%202019- 2021_12.2018.pdf

* For all combinations of zoonotic agents and matrix (Food, Feed and Animals) for ‘Prevalence’ and ‘Disease Status’: one text form reported per each combination of matrix/zoonoses or zoonotic agent (a): Sampling scheme (sampling strategy, frequency of the sampling, type of specimen taken, methods of sampling (description of sampling techniques) + testing scheme (case definition, diagnostic/analytical methods used, diagnostic flow (parallel testing, serial testing) to assign and define cases. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (b): The control program/strategies in place, including vaccination if relevant. If applicable a description of how eradication measures are/were implemented, measures in case of the positive findings or single cases; any specific action decided in the Member State or suggested for the European Union as a whole on the basis of the recent/current situation, if applicable. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (c): Mandatory: Yes/No. (d): Minimum five years. (e): Relevance of the findings in animals to findings in foodstuffs and for human cases (as a source of infection).

Description of Monitoring/Surveillance/Control programmes system*: Rabies 1. Monitoring/Surveillance/Control programmes system(a)

The aim of the rabies National Program for the Prevention, Surveillance, Control and Eradication in Bulgaria for 2019-2021, is to eradicated rabies on the country territory by implementing of oral vaccination of foxes in the 50 km vaccination zones on the Bulgarian land borders (North, West and South land borders) and 20 km land border along the Danube River. Vaccination is performed twice a year in spring (march-may) and autumn (September-November) with vaccine bites. The vaccination will be implemented on a total territory of 57 019, 473 km2 in 22 administrative regions as follows: Pernik, Smolyan, Kyustendil, Kurdjali, Haskovo, Dobrich, Silistra, Vidin, Montana, Blagoevgrad, Pazardjik, Yambol, Burgas, Plovdiv, St. Zagora, Sofia, Shumen, Razgrad, Ruse, Veliko Tarnovo, Pleven, Vratza (Map 1)

2 campaigns of oral vaccination are conducted in 2019 as follows:

Spring vaccination – March – April 2019

Number Average per sq. Area of vac. Km

22 Bulgaria

Distribution by air

Total 60017 1425900 23,76

Manual distribution Sofia Montana 4300 12000 2,79 Autumn vaccination- November-December 2019

Number Average per sq. Area of vac. Km

Distribution by air

Total 62278 1426100 22,90

Manual distribution Sofia Montana 4300 12000 2,79

The laboratory control of the oral vaccination is done at the National Diagnostic and research veterinary Medical Institute (NDRVMI) by: - IFT test – direct immunofluorescent test for virus detection - ELISA – for Ab-es detection - Tetracycline biomarker Test - Virus isolation

Map 1. Zones for oral vaccination of wild animals in Bulgaria

The data for rabies positive cases in domestic and wild animals in Bulgaria by years for the period 2004 – 2017 are presented in the table below:

23 Bulgaria

Year Number of District (s) positive animals (domestic; wild)

2004 10 Vidin, Veliko Tarnovo, Dobrich, Vtarza, Pleven 2005 11 Vidin, Veliko Tarnovo, Dobrich, Montana, Silistra, 2006 9 Varna, Dobrich, Montana, Razgrad, Shumen 2007 40 Varna, Veliko Tarnovo, Vtarza, Razgrad, Silistra, Dobrich, Sofia, Turgovishte, Shumen 2008 49 Varna, Vidin, Veliko Tarnovo, Vtarza, Kyustendil, Lovech, Montana, Pernik, Pleven, Razgrad, Ruse, Silistra, Sofia, Shumen 2009 59 Sofia, Vidin, Vtatza, Montana, Kyustendil, Pleven, Razgrad, Silistra, Turgovishte, Shumen, Burgas, Plovdiv 2010 6 Vtarza, veliko tarnovo, Ruse and Shumen 2011 1 Kyustendil

2012 1 Kyustendil

2013 0 2014 2 Blagoevgrad 2015 0 2016 0 2017 0

2. Measures in place(b)

n/a 3. Notification system in place to the national competent authority(c)

n/a 4. Results of investigations and national evaluation of the situation, the trends (d) and sources of infection(e)

n/a 5. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_6_Prilojenie_6%20Programme_Rabies_2019%20- %202021.pdf * For all combinations of zoonotic agents and matrix (Food, Feed and Animals) for ‘Prevalence’ and ‘Disease Status’: one text form reported per each combination of matrix/zoonoses or zoonotic agent (a): Sampling scheme (sampling strategy, frequency of the sampling, type of specimen taken, methods of sampling (description of sampling techniques) + testing scheme (case definition, diagnostic/analytical methods used, diagnostic flow (parallel testing, serial testing) to assign and define cases. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (b): The control program/strategies in place, including vaccination if relevant. If applicable a description of how eradication measures are/were implemented, measures in case of the positive findings or single cases; any specific action decided in the Member State or suggested for the European Union as a whole on the basis of the recent/current situation, if applicable. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (c): Mandatory: Yes/No. (d): Minimum five years. (e): Relevance of the findings in animals to findings in foodstuffs and for human cases (as a source of infection).

Description of Monitoring/Surveillance/Control programmes system*: Q - Fever

24 Bulgaria

1. Monitoring/Surveillance/Control programmes system(a)

Programme range:  Random laboratory testing for antibodies (Abs) against Coxiella burnetii in blood samples from bovines and small ruminants (sheep and goats) from endemic regions and newly identified infected regions on the country territory;  Serological testing of ruminants in case of confirmed human cases;  Annual double serological testing of all male breeding bovines in the regional reproduction centres;  Laboratory testing of blood samples from animals originated from herds with higher percentage of abortions when Coxiella burnetii is detected by PCR.

2. Measures in place(b)

n/a 3. Notification system in place to the national competent authority(c)

n/a 4. Results of investigations and national evaluation of the situation, the trends (d) and sources of infection(e)

n/a 5. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_10_Prilojenie_10%20Q_fever%202019%20-%202021.pdf

* For all combinations of zoonotic agents and matrix (Food, Feed and Animals) for ‘Prevalence’ and ‘Disease Status’: one text form reported per each combination of matrix/zoonoses or zoonotic agent (a): Sampling scheme (sampling strategy, frequency of the sampling, type of specimen taken, methods of sampling (description of sampling techniques) + testing scheme (case definition, diagnostic/analytical methods used, diagnostic flow (parallel testing, serial testing) to assign and define cases. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (b): The control program/strategies in place, including vaccination if relevant. If applicable a description of how eradication measures are/were implemented, measures in case of the positive findings or single cases; any specific action decided in the Member State or suggested for the European Union as a whole on the basis of the recent/current situation, if applicable. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (c): Mandatory: Yes/No. (d): Minimum five years. (e): Relevance of the findings in animals to findings in foodstuffs and for human cases (as a source of infection).

Description of Monitoring/Surveillance/Control programmes system*: West Nile Fever 1. Monitoring/Surveillance/Control programmes system(a)

Programme range:  laboratory testing of blood samples from sentinel animals (equids and poultry) for Abs detection against WNFV;  laboratory testing of blood samples from wild birds and dogs for Abs detection against WNFV;  laboratory testing of blood samples, organ suspensions and brain, and insects samples (mosquitoes) for WNFV genome detection;  morphological identification of insects (mosquitoes);  clinical investigation of susceptible animals, on a montly basis in 6 administrative regions of Bulgaria (Burgas, Jambol, Haskovo, Kurdjali, Smolian and Blagoevgrad)

25 Bulgaria

2. Measures in place(b)

n/a 3. Notification system in place to the national competent authority(c)

n/a 4. Results of investigations and national evaluation of the situation, the trends (d) and sources of infection(e)

n/a 5. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_19_Prilojenie_19%20Programme%20WNF_2019%20- %202021.pdf * For all combinations of zoonotic agents and matrix (Food, Feed and Animals) for ‘Prevalence’ and ‘Disease Status’: one text form reported per each combination of matrix/zoonoses or zoonotic agent (a): Sampling scheme (sampling strategy, frequency of the sampling, type of specimen taken, methods of sampling (description of sampling techniques) + testing scheme (case definition, diagnostic/analytical methods used, diagnostic flow (parallel testing, serial testing) to assign and define cases. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (b): The control program/strategies in place, including vaccination if relevant. If applicable a description of how eradication measures are/were implemented, measures in case of the positive findings or single cases; any specific action decided in the Member State or suggested for the European Union as a whole on the basis of the recent/current situation, if applicable. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (c): Mandatory: Yes/No. (d): Minimum five years. (e): Relevance of the findings in animals to findings in foodstuffs and for human cases (as a source of infection).

Description of Monitoring/Surveillance/Control programmes system*: Salmonella in breeding flocks of Gallus Gallus 1. Monitoring/Surveillance/Control programmes system(a) The programme scope is to determine the health status of Gallus Gallus breeding flocks in Bulgaria in respect of Salmonella Enteritis, Salmonella Hadar, Salmonella infantis, Salmonella typhimurium (including monophasic S. typhimurium serotypes with the formula 1,4, (5), 12: i), Salmonella Virchow, and all other Salmonella species.

Programme range: The official sampling within the National Control Programme is done by official veterinarians. The samples are analysed by accredited laboratories (ISO/IEC 17025:2005), designated and approved by BFSA to carry out testing official and FBO samples on Salmonella in poultry within SCP. All laboratories are designated in accordance with Article 12 of Regulation (EC) No 2160/2003. The programme activities include detection of Salmonella in animal faeces, tissue, environmental samples, feed and water and serotyping.

26 Bulgaria

All samples for the purpose of the official control have to be tested in accredited state laboratories and all positive or doubtful results have to be confirmed in the Bulgarian NRL for salmonella within National Diagnostic and Research veterinary Institute in Sofia.

The samples in the frame of self-control can be tested in approved private ot state laboratories.

Sampling for official control:

1. Sampling in hatchery by initiative of the FBO: - Routine sampling each 16 weeks in the hatchery; - Routine sampling in the farm – 2 time per production cycle: once during the first 4 weeks (after moving to laying hens) and second time during the laying period but not later than 8 weeks before ending the production cycle; - For each flock the total number of the official sampling per one production cycle is 2 in the farm and 3 in the hatchery; or 3 official samplings per flock per year. - The number of the samples for official sampling is 2 samples per flock in the farm and 1 sample from hatchery 2. Sampling in the farm by initiative of the FBO: -sampling within 4 weeks after moving to laying hens; -during the laying period but not later than 8 weeks before ending the production cycle; -during the production period outside the cases pointed above; -For each flock the total number of the official sampling per one production cycle have to be 3; or 2 official samplings per calendar year; 3. Confirmatory sampling and control of antimicrobials, inhibitors and disinfectants: In case of positive flock (confirmed by the NRL), official sampling for result confirmation is done solely by official veterinarian.

The samples have to be taken as follows:

- 7 samples: 5 boot swabs/faeces and 2 dust samples; or

- bacteriological testing of organ samples (caecum) from 300 carcases; or

- 4000 eggs

Treatment with antimicrobials is not allowed in the frame of the Salmonella National control programmes! In case of suspected treatment with antimicrobials or disinfectants samples have to be taken as follows: 5 carcases (for detection of antimicrobials residues) and boot swabs, egg shells etc. for inhibitors/disinfectants detection.

2. Measures in place(b) More detailed information is available at the BFSA website on the following link: http://bfsa.bg/bg/Page/NPBJZ19-21/index/NPBJZ19- 21/%D0%9D%D0%B0%D1%86%D0%B8%D0%BE%D0%BD%D0%B0%D0%BB%D0%BD%D0%B0

3. Notification system in place to the national competent authority(c)

n/a 4. Results of investigations and national evaluation of the situation, the trends (d) and sources of infection(e)

27 Bulgaria

n/a 5. Additional information

n/a * For all combinations of zoonotic agents and matrix (Food, Feed and Animals) for ‘Prevalence’ and ‘Disease Status’: one text form reported per each combination of matrix/zoonoses or zoonotic agent (a): Sampling scheme (sampling strategy, frequency of the sampling, type of specimen taken, methods of sampling (description of sampling techniques) + testing scheme (case definition, diagnostic/analytical methods used, diagnostic flow (parallel testing, serial testing) to assign and define cases. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (b): The control program/strategies in place, including vaccination if relevant. If applicable a description of how eradication measures are/were implemented, measures in case of the positive findings or single cases; any specific action decided in the Member State or suggested for the European Union as a whole on the basis of the recent/current situation, if applicable. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (c): Mandatory: Yes/No. (d): Minimum five years. (e): Relevance of the findings in animals to findings in foodstuffs and for human cases (as a source of infection).

Description of Monitoring/Surveillance/Control programmes system*: Salmonella in broiler flocks of Gallus Gallus 1. Monitoring/Surveillance/Control programmes system(a) Programme range: The scope of the programme is to determine the health status of Gallus Gallus broiler flocks in Bulgaria in respect and to reduce the infection until less than 1% with regard to Salmonella Enteritis and Salmonella typhimurium (including monophasic S. typhimurium serovar 1,4/5/,1 2:i The official sampling within the National Control Programme is done by the official veterinarians within the BFSA structure. The samples are analysed by accredited laboratories (ISO/IEC 17025:2005), designated and approved by BFSA to carry out testing official and FBO samples on Salmonella in poultry within SCP. All laboratories are designated in accordance with Article 12 of Regulation (EC) No 2160/2003. The programme activities include detection of Salmonella in animal faeces, tissue, environmental samples, feed and water and serotyping.

All samples for the purpose of the official control have to be tested in accredited state laboratories and all positive or doubtful results have to be confirmed in the Bulgarian NRL for salmonella within National Diagnostic and Research veterinary Institute in Sofia. The samples in the frame of self-control can be tested in approved private or state laboratories. The programme is implemented in the entire country in all 28 administrative districts in Bulgaria, excluding farms with less than 2000 birds and for own consumption. There is no practice for vaccination against Salmonella in broiler flocks in Republic of Bulgaria.

Sampling scheme for the purpose of the official control: The competent authority (BFSA) in comply with Commission Regulation (EC) № 200/2012 select randomly each year 10% from the poultry farms with more than 5000 birds, taking into account the farm size and its geographical location. Samples are taken from a randomly selected flock with max. 3 weeks to slaughtering. At least 2 swabs (ore one of the samples can be dust sample). The samples for self-control are taken by the farm owner (or private veterinarian). Samples for self- control have to be taken from all broiler flocks included in the Control programme, 3 weeks before slaughter. The sampling scheme for the purpose of the official control and self-control is presented on the table below: Sampling place Sampling unit sampler Sampling time Sample type

28 Bulgaria

2 boot swabs/ faeces (2х 75 g.) 1 pool sample per FBO/official 2-3 weeks before flock; farm flock control slaughter Or 1 set of boot swabs and 1 dust sample

2. Measures in place(b)

n/a 3. Notification system in place to the national competent authority(c)

n/a 4. Results of investigations and national evaluation of the situation, the trends (d) and sources of infection(e)

n/a 5. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19-21/3_14_Prilojenie_14%20Salmonella%20broilers%202019- 2021_16.10.2018.pdf * For all combinations of zoonotic agents and matrix (Food, Feed and Animals) for ‘Prevalence’ and ‘Disease Status’: one text form reported per each combination of matrix/zoonoses or zoonotic agent (a): Sampling scheme (sampling strategy, frequency of the sampling, type of specimen taken, methods of sampling (description of sampling techniques) + testing scheme (case definition, diagnostic/analytical methods used, diagnostic flow (parallel testing, serial testing) to assign and define cases. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (b): The control program/strategies in place, including vaccination if relevant. If applicable a description of how eradication measures are/were implemented, measures in case of the positive findings or single cases; any specific action decided in the Member State or suggested for the European Union as a whole on the basis of the recent/current situation, if applicable. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (c): Mandatory: Yes/No. (d): Minimum five years. (e): Relevance of the findings in animals to findings in foodstuffs and for human cases (as a source of infection).

Description of Monitoring/Surveillance/Control programmes system*: Salmonella in laying hens 1. Monitoring/Surveillance/Control programmes system(a) The scope of the programme is to determine the health status of Gallus Gallus laying hens flocks in Bulgaria in respect Salmonella Enteritis and Salmonella Typhimurium (including monophasic S. typhimurium serovar 1,4/5/,1 2:i The official sampling within the National Control Programme is done by the official veterinarians within the BFSA structure. The programme activities include detection of Salmonella in animal faeces, tissue, environmental samples, feed and water and serotyping. The samples are analysed by accredited laboratories (ISO/IEC 17025:2005), designated and approved by BFSA to carry out testing official and FBO samples on Salmonella in poultry within SCP. All laboratories are designated in accordance with Article 12 of Regulation (EC) No 2160/2003.

29 Bulgaria

All samples for the purpose of the official control have to be tested in accredited state laboratories. The samples in the frame of self-control can be tested in approved private laboratories. All positive or doubtful results have to be confirmed in the Bulgarian NRL for salmonella within National Diagnostic and Research veterinary Institute in Sofia.

Sampling scheme: The official sampling is performed by official veterinarian in accordance with Annex to Commission regulation (ЕС) № 517/2011, e.g. Per flock (with min. 1000 birds): 1 official sampling during rearing period and second sampling 2 weeks before slaughter/for one production period.

Each official sampling consists of at least 2 samples (+ 1 additional dust/faecal sample).

It is expected to have at least 1 official sampling per year/ per flock (min 1000 birds).

The samples for self-control are taken by the farm owner or private veterinarian in relation with the approved Salmonella control plan

The sampling scheme is described in the table below:

Laying hens (GALLUS GALLUS)

Samplin Sampling unit Sampler Sampling time Sample type place

Laying hens – rearing period

Farm Batch owner 1 day old Feasec from 5 units (to be tested as a pool samle)

Farm flock owner 4 weeks 2 pars of boot swabs (to be tested възраст as a pool samle per each flock)

Or

2x150 samples/ 5х 60 samples of fresh feaces (to be tested as a pool samle per each flock)

Farm flock Owner / 2 weeks 2 pars of boot swabs (to be tested official vet before as a pool samle per each flock) movement to egg Or production 5х 60 samples of fresh feaces (to group be tested as a pool samle per each flock)

Laying hens - Adults

30 Bulgaria

Farm flock Owner 22-26 week 2 pars of boot swabs (to be tested as a pool samle per each flock)

Or

5х 60 samples of fresh feaces (to be tested as a pool samle per each flock)

Farm flock Official control Once per year 2 pars of boot swabs (to be tested as a pool samle per each flock)

Or

2x150 samples/ 5х 60 samples of fresh feaces (to be tested as a 2 individual samles per each flock)

In addition:

1 dust sample (100 grams) or 1 fecal sample

Farm flock Owner/Official 2 weeks 2 pars of boot swabs (to be tested control before as a pool samle per each flock) slaughter Or

2x150 samples/ 5х 60 samples of fresh feaces (to be tested as a pool samle per each flock)

2. Measures in place(b)

n/a 3. Notification system in place to the national competent authority(c)

n/a 4. Results of investigations and national evaluation of the situation, the trends (d) and sources of infection(e)

n/a 5. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19- 21/3_15_Prilojenie_15%20Salmonella%20laying%20hens%202019-2021_16.10.2018.pdf * For all combinations of zoonotic agents and matrix (Food, Feed and Animals) for ‘Prevalence’ and ‘Disease Status’: one text form reported per each combination of matrix/zoonoses or zoonotic agent (a): Sampling scheme (sampling strategy, frequency of the sampling, type of specimen taken, methods of sampling (description of sampling techniques) + testing scheme (case definition, diagnostic/analytical methods used, diagnostic flow (parallel testing, serial testing) to assign and define cases. If programme approved by the EC, please provide link to the specific programme in the Commission`s website.

31 Bulgaria

(b): The control program/strategies in place, including vaccination if relevant. If applicable a description of how eradication measures are/were implemented, measures in case of the positive findings or single cases; any specific action decided in the Member State or suggested for the European Union as a whole on the basis of the recent/current situation, if applicable. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (c): Mandatory: Yes/No. (d): Minimum five years. (e): Relevance of the findings in animals to findings in foodstuffs and for human cases (as a source of infection).

Description of Monitoring/Surveillance/Control programmes system*: Salmonella in Turkeys 1. Monitoring/Surveillance/Control programmes system(a)

The scope of the programme is to determine the health status of turkey flocks in Bulgaria in respect Salmonella Enteritis and Salmonella Typhimurium (including monophasic S. typhimurium serovar 1,4/5/,1 2:i) and to keep the % of Salmonella positive flocks as follows: Turkey – breeding flocks – less than 1%; Fattening turkeys - less than 1%;

Sampling scheme:

Official sampling: The sampling for the purpose of the official control covers all flocks of Fattening turkeys and all breeding flocks. The sampling scheme is in compliment with the Commission Regulation (ЕС) 1190/2012.

Turkey – breeding flocks – yearly sampling of all breeding flocks with min. 250 adults (between 30-45 weeks of age) from farms for grandparent breeding flocks and parent breeding flocks. Fattening turkeys – from farms with min. 550 fattening turkeys – sampling of at least one flock in the farm For 2017 and 2018 sampling of different categories of turkey for Salmonella are in accordance with Commission Regulation (ЕС) 2160/2003 and Commission Regulation (ЕС) 517/2011. Self-control sampling: The sampling for the purpose of self-control is as follows: breeding flocks during rearing period – from each flock– one day old, 4 weeks of age and 2 weeks before movement to egg production group; breeding flocks adults – from each flock – each 3 weeks during the production period in the farm; Fattening turkeys – from each flock– 3 weeks before slaughter (official sampling can replace the sampling for self-control). Farm sailing their production directly to the consumer – from each flock – one sampling for the entire production period The sampling scheme, the type of samples for the purpose of the official control and self-contrrol is presented in the table below: Turkey

Samplin Sampling Sampler Sampling time Sample type place unit

Fattening turkey

farm Flock Owner/official control 3 weeks before 2 boot swabs/feaces (2х slaughter 75 г.) (to be tested as a pool samle per each flock)

32 Bulgaria

Or

2 boot swabs and 1 dust sample per each flock

Turkey – breeding flocks

breeding flokcks during rearing period

farm Flock Owner 1 day old feaces/environmental samples from each unit, (to be tested as a pool samle per each flock)

farm Flock Owner 4 weeks of age per each flock:

fresh faeces from selected arear of the premises (min 1 g, from one place, or sample of 200-300 g from the entire premises depending on the number of birds) –to be tested as min. 2 pool samples or 5 boot samples, to be tested as 2 pool samples

or 1 pair of boot swabs + dust sample (900 cm²) or

2х150 g faeces to be tested as 2 pool samples

farm Flock Owner 2 weeks before per each flock: movement fresh faeces from selected arear of the premises (min 1 g, from one place, or sample of 200-300 g from the entire premises depending on the number of birds) –to be tested as min. 2 pool samples or 5 boot samples, to be tested as 2 pool samples

33 Bulgaria

or 1 pair of boot swabs + dust sample (900 cm²) or

2х150 g faeces to be tested as 2 pool samples

breeding flocks adults

farm Flock Owner Each 16 weeks per each flock:

fresh faeces from selected arear of the premises (min 1 g, from one place, or sample of 200-300 g from the entire premises depending on the number of birds) –to be tested as min. 2 pool samples or 5 boot samples, to be tested as 2 pool samples

or 1 pair of boot swabs + dust sample (900 cm²) or

2х150 g faeces to be tested as 2 pool samples

farm Flock Owner 3 weeks before per each flock: slaughter fresh faeces from selected arear of the premises (min 1 g, from one place, or sample of 200-300 g from the entire premises depending on the number of birds) –to be tested as min. 2 pool samples or 5 boot samples, to be tested as 2 pool samples

or 1 pair of boot swabs + dust sample (900 cm²) or

2х150 g faeces to be tested as 2 pool samples

farm Flock Official control Single per each flock: sampling

34 Bulgaria

fresh faeces from selected arear of the premises (min 1 g, from one place, or sample of 200-300 g from the entire premises depending on the number of birds) –to be tested as min. 2 pool samples or 5 boot samples, to be tested as 2 pool samples

or 1 pair of boot swabs + dust sample (900 cm²) or

2х150 g faeces to be tested as 2 pool samples

Farms with turkeys during rearing period or sailing their production directly to the consumer

farm Flock Owner Single per each flock: sampling fresh faeces from selected arear of the premises (min 1 g, from one place, or sample of 200-300 g from the entire premises depending on the number of birds) –to be tested as min. 2 pool samples or 5 boot samples, to be tested as 2 pool samples

or 1 pair of boot swabs + dust sample (900 cm²) or

2х150 g faeces to be tested as 2 pool samples

Official control of feeding stuffs for poultry:

The official control of feed is undertaken by authorised experts in the Regional Food safety directorates of BFSA.

 Sampling in producers premises etc.;  Sampling in the farm 2. Measures in place(b)

n/a

35 Bulgaria

3. Notification system in place to the national competent authority(c)

n/a 4. Results of investigations and national evaluation of the situation, the trends (d) and sources of infection(e)

n/a 5. Additional information

More information is available on the BFSA website at the following link: http://bfsa.bg/userfiles/files/ZJ/DPP19- 21/3_16_Prilojenie_16%20Salmonella%20tur%D0%BAey%202019-2021_16.10%202018.pdf * For all combinations of zoonotic agents and matrix (Food, Feed and Animals) for ‘Prevalence’ and ‘Disease Status’: one text form reported per each combination of matrix/zoonoses or zoonotic agent (a): Sampling scheme (sampling strategy, frequency of the sampling, type of specimen taken, methods of sampling (description of sampling techniques) + testing scheme (case definition, diagnostic/analytical methods used, diagnostic flow (parallel testing, serial testing) to assign and define cases. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (b): The control program/strategies in place, including vaccination if relevant. If applicable a description of how eradication measures are/were implemented, measures in case of the positive findings or single cases; any specific action decided in the Member State or suggested for the European Union as a whole on the basis of the recent/current situation, if applicable. If programme approved by the EC, please provide link to the specific programme in the Commission`s website. (c): Mandatory: Yes/No. (d): Minimum five years. (e): Relevance of the findings in animals to findings in foodstuffs and for human cases (as a source of infection).

Food-borne Outbreaks 1. System in place for identification, epidemiological investigations and reporting of food-borne outbreaks For each case of food-borne outbreak the healthcare experts notify the State heath control directorate (SHCD) of the Ministry of health (MH) in Bulgaria. Joined team of experts from the Ministry of health and the Ministry of agriculture, food and forestry of Bulgaria (MAFF) is established to conduct an epidemiological investigation of each case (food-borne outbreak). A report from each epidemiological investigation is send to the MH of Bulgaria with epidemiological analysis, measures taken, including hygiene measures on behalf of MAFF. The State heath control directorate of MH keeps an up to date register of FBOs in Bulgaria and prepares an annual report for all FBOs on the country territory to the Minister of Health. 2. Description of the types of outbreaks covered by the reporting The reporting of FBOs in Bulgaria covers all food borne outbreaks in restaurants, Kinder gardens, kitchens with catering activities, catering services and home FBOs.

3. National evaluation of the reported outbreaks in the country(a)

The evaluation is in the competences of the Ministry of Health of Bulgaria 4. Descriptions of single outbreaks of special interest

N/A 5. Control measures or other actions taken to improve the situation As a control measures a risk analysis of each case of FBO is done in the premises they are notified and as well as re-evaluation of the level of risk in those premises. Enhanced control by the Ministry of health and the Ministry of health and the Ministry of agriculture, food and forestry of Bulgaria. 6. Any specific action decided in the Member State or suggested for the European Union as a whole on the basis of the recent/current situation

36 Bulgaria

N/A 7. Additional information

N/A (a): Trends in numbers of outbreaks and numbers of human cases involved, relevance of the different causative agents, food categories and the agent/food category combinations, relevance of the different type of places of food production and preparation in outbreaks, evaluation of the severity of the human cases.

Description of Monitoring/Surveillance/Control programmes system*:

1. Monitoring/Surveillance/Control programmes system(a)

Program for monitoring and reporting of antimicrobial resistance in zoonotic and commensal bacteria (notified under document C(2013) 7145)(2013/652/EU) – in 2019 in Bulgaria caeca samples (153) representing the same number of epidemiological units of slaughtered pigs were tested for isolation of commensal E.coli and presence of ESBL/AmpC/ Carbapenemase producing E.coli. Also all Salmonella spp. Isolated from pig and bovine carcasses, collected according to Regulation 2073:2005 requirements were tested. Fresh chilled pig and bovine meat from retail were tested for presence of ESBL/AmpC/ Carbapenemase producing E.coli.

2. Measures in place(b)

na

3. Notification system in place to the national competent authority(c)

na

4. Results of investigations and national evaluation of the situation, the trends (d) and sources of infection(e)

na

5. Additional information

na

* For all combinations of zoonotic agents and matrix (Food, Feed and Animals) for ‘Prevalence’ and ‘Disease Status’: one text form reported per each combination of matrix/zoonoses or zoonotic agent

(a): Sampling scheme (sampling strategy, frequency of the sampling, type of specimen taken, methods of sampling (description of sampling techniques) + testing scheme (case definition, diagnostic/analytical methods used, diagnostic flow (parallel testing, serial testing) to assign and define cases. If programme approved by the EC, please provide link to the specific programme in the Commission`s website.

(b): The control program/strategies in place, including vaccination if relevant. If applicable a description of how eradication measures are/were implemented, measures in case of the positive findings or single cases; any specific action decided in the Member State or suggested for the European Union as a whole on the basis of the recent/current situation, if applicable. If programme approved by the EC, please provide link to the specific programme in the Commission`s website.

(c): Mandatory: Yes/No.

37 Bulgaria

(d): Minimum five years.

(e): Relevance of the findings in animals to findings in foodstuffs and for human cases (as a source of infection).

38 Bulgaria

Institutions and laboratories involved in antimicrobial resistance monitoring and reporting

In AMR program for 2019 were involved different departments and laboratories of Bulgarian Food Safety Agency. Starting with Department of Food Control in Head quarter of BFSA which organised collecting and transporting of caeca pig samples from slaughterhouses and pig and bovine meat from retail of 25 administrative units, covering more than 95% of human population in R.Bulgaria. Also NRL Salmonella, campylobacter, staphylococci and antimicrobial resistance was involved in receiving of samples, isolation and identification of microorganisms set up in monitoring program. NRL done testing of isolates of Salmonella spp., commensal E.coli and ESBL/AmpC/Carbapenemase producing E.coli and Salmonella spp. NRL also did reporting of data received form AMR monitoring program to Centre of Risk Assessment of Food Chain to be reported to EFSA.

Short description of the institutions and laboratories involved in data collection and reporting

General Antimicrobial Resistance Evaluation

1. Situation and epidemiological evolution (trends and sources) regarding AMR to critically important antimicrobials(a) (CIAs) over time until recent situation

na

2. Public health relevance of the findings on food-borne AMR in animals and foodstuffs

na

3. Recent actions taken to control AMR in food producing animals and food

na

4. Any specific action decided in the Member State or suggestions to the European Union for actions to be taken against food-borne AMR threat

na

5. Additional information

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(a): The CIAs depends on the bacterial species considered and the harmonised set of substances tested within the framework of the harmonised monitoring:

• For Campylobacter spp., macrolides (erythromycin) and fluoroquinolones (ciprofloxacin);

• For Salmonella and E. coli, 3rd and 4th generation cephalosporins (cefotaxime) and fluoroquinolones (ciprofloxacin) and colistin (polymyxin);

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General Description of Antimicrobial Resistance Monitoring*;

Caeca samples from slaughtered pig – 153, Fresh pig meat – 150 and fresh bovine meat – 150 samples; Commensal E.coli from caeca pig samples, ESBL/AmpC/Carbapenemase producing E.coli from caeca pig samples, ESBL/AmpC/Carbapenemase producing E.coli from pig and bovine meat

1. General description of sampling design and strategy(a)

The information reported for commensal E.coli and ESBL/AmpC/Carbapenemase producing E.coli, collected from whole territory of the country and then isolated from caeca samples of slaughtered pigs is representative for 153 epidemiological units from whole territory of R. of Bulgaria for period January – December 2019. Tested Salmonella spp. Isolates - 4 originated from pig and bovine carcasses according to requirements of Regulation 2073:2005 are representative for whole industry for slaughtering and processing of pig and bovine meat in Bulgaria, and also 4 other isolates from pig meat. We have luck of Salmonella isolates from pigs and bovines.

Program covered only group of animals (fattened pigs) according to the Decision from 2013/652/EU.

Program for monitoring and reporting of antimicrobial resistance in zoonotic and commensal bacteria (notified under document C(2013) 7145)(2013/652/EU) – in 2017 in Bulgaria caeca samples (153) representing the same number of epidemiological units of slaughtered pigs were tested for isolation of commensal E.coli and presence of ESBL/AmpC/ Carbapenemase producing E.coli. Also all Salmonella spp. Isolated from pig and bovine carcasses, collected according to Regulation 2073:2005 requirements were tested. Fresh chilled pig and bovine meat from retail were tested for presence of ESBL/AmpC/ Carbapenemase producing E.coli.

Method of sampling (caeca samples – from cecum of slaughtered pigs, aseptically, immediately send to laboratory for testing; meat samples – from retail – packed or fresh chilled pig and bovine meat sale in meat shops or supermarkets. Samples were collected by official control inspectors), Frequency of sampling (Each sample represent one single epidemiological unit, all samples were designed to be collected through all seasons of the year. Each month in laboratory received the same number of caeca and meat samples. Procedure of examination was started immediately after receiving of samples, 101 commensal E.coli isolates were tested and represent 101 different epidemiological units (pig farms). All ESBL/AmpC/Carbapenemase producing E.coli from caeca pig samples and from pig and bovine meat were tested with panel 1 EUVSEC and panel 2 EUVSEC 2 and data were filled in Excel for reporting of DCF system of EFSA.

Bacteria - Salmonella spp.

Origin of the isolates - Carcases of fattening pigs sampled for testing and verification of compliance, in accordance with point 2.1.4 of Chapter 2 of Annex I to Regulation (EC) No 2073/2005

Sampling unit type(a) - Slaughter batch

Sampling stage - Slaughterhouse

Sample type - Food sample carcase swabs

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Sampling context - Monitoring

Sampler - HACCP and own checks and/or Official sampling

Sampling strategy - Objective sampling

Programme code - AMR MON and when it is the case(e) AMR MON pnl2

Bacteria - Indicator commensal E . coli

Origin of the isolates - Caecal samples gathered at slaughter from fattening pigs

Sampling unit type(a) - Slaughter batch

Sampling stage - Slaughterhouse

Sample type - Animal sample caecum

Sampling context - Monitoring

Sampler - Official sampling

Sampling strategy - Objective sampling

Programme code - AMR MON and when it is the case(e) AMR MON pnl2 Bacteria - Specific monitoring ESBL-/AmpC/or carbapenemase-producing E . coli Origin of the isolates - Caecal samples gathered at slaughter from fattening pigs and samples of fresh pig meat and bovine meat gathered at retail Sampling unit type(a) - Slaughter batch or batch for fresh meat Sampling stage – Slaughterhouse and retail for fresh meat Sample type - Animal sample caecum and Food sample - meat Sampling context - Monitoring Sampler - Official sampling Sampling strategy - Objective sampling Programme code - AMR MON and when it is the case(e) AMR MON pnl2

2. Stratification procedure per animal population and food category

Samples of 153 caeca content of slaughtered pigs were collected in 63 pig slaughterhouses, each sample representing a separate epidemiological unit (pig farm). Bulgaria had to collect samples of not less than 150 epidemiological units. The samples were collected in 63 slaughterhouses, and information was previously collected for the previous 2018 for the separately registered pig farms and where they slaughter their animals. In the selected 63 slaughterhouses we had the opportunity to collect samples from over 200 epidemiological units. The number of the farm of origin was also recorded in the slaughterhouses where the samples were taken, so as not to duplicate the sample. The number of the farm / epidemiological unit was recorded in the laboratory and if a sample came from it again, it was dropped from testing. Samples were evenly distributed throughout the year and covered all seasons. The production of slaughterhouses included in annual program produces over 90% of fresh pork as national production.

The freshly chilled meat of pigs and calves is collected from the shops of 25 out of the 28 administrative territorial units of the Republic of Bulgaria where it lives over 92% the population of the country. Samples of fresh pork and beef were taken from various stores (large retail chains and specialized meat shops),

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taking care not to repeat the batch numbers and date of production.Samples were distributed evenly during the year, observing the random selection principle.

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3. Randomisation procedure per animal population and food category

During the preparation of the program, full information was collected on the number of registered pig slaughterhouses and their annual throughput of their production for the previous year, information was gathered on how many pig farms and when they sent pigs for slaughter to the slaughterhouse concerned. Information was collected on the number of registered and operating pig holdings for fattening pigs and where they send the fattened animals for slaughter. A sampling scheme was drawn up by pig slaughterhouses included in the program so that the epidemiological units are not repeated during sampling. Samples of meat from pigs and cattle were distributed in 25 administrative territorial units of the country where more than 92% of the population lives.

The distribution of pig faecal samples as well as fresh pork and beef samples are included in the tables. These tables include all slaughterhouses for pigs from which faecal samples will be collected, and for each slaughterhouse the registration numbers of the respective farms that send animals for slaughter are recorded. Ie the official doctor, after taking a sample from the specified farm, will wait for the arrival of a batch of animals from another non-recurring farm and will take a sample again. For countries like Bulgaria with a small number of pig farms, it is a great challenge not to re-sample from the same farm, but this problem, although with great effort, was understood and kept under control. Faecal samples from cattle up to 1 year of age were not taken because Bulgaria has a small production of beef. The samples were evenly distributed over months so that the average monthly number ranged from 12 to 15 samples. In some cases, there was a later or earlier dispatch of the sample due to the earlier or later dispatch of animals for slaughter from the relevant farm / epidemiological unit. A leading factor in the collection of faecal samples was the fact that a sample from the same epidemiological unit was not allowed to be repeated.

With regard to the samples of meat from the trade network of the country, there were no difficulties for their distribution in 25 administrative units and their distribution by months, so that each month an average of 12-14 samples of meat are taken so that annually collect a total of 150 samples.

With regard to swine salmonella reported under the Program, no further information can be provided because the NRL has the relevant salmonella isolates and the accompanying document from which matrix (carcases) they are isolated.

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4. Analytical method used for detection and confirmation(b)

Analytical method used for detection and confirmation: according to the legislation, the protocols developed by the EURL-AR used and reported here.

5. Laboratory methodology used for detection of antimicrobial resistance(C)

ISO standard 20776-1:2006

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EUVSEC and EUVSEC 2 panel for testing were used for E.coli from caeca pig samples and from pig and bivene meat and Salmonella spp. isolates from pig and bovine carcasses, according to requirements of Regulation 2073.

6. Results of investigation

104 commensal E.coli isolates from 153 caeca pig samples, 81 ESBL/AmpC/Carbapenemase producing E.coli from 153 caeca pig samples, 28 ESBL/AmpC/Carbapenemase producing E.coli isolates from 150 pig meat, 36 ESBL/AmpC/Carbapenemase producing E.coli isolates from 150 bovine meatsamples, 4 isolates of Salmonella spp. From pig carcasses and 4 Salmonella spp. Isolates from pig meat. 7. Additional information

n/a

* to be filled in per combination of bacterial species/matrix

(a): Method of sampling (description of sampling technique: stage of sampling, type of sample, sampler), Frequency of sampling, Procedure of selection of isolates for susceptibility testing, Method used for collecting data.

(b): Analytical method used for detection and confirmation: according to the legislation, the protocols developed by the EURL-AR should be used and reported here. In the case of the voluntary specific monitoring on Carbapenemase-producers, the selective media used (commercial plates, ‘in house’ media) should be also reported here. In general, any variation with regard to the EURL-AR protocols should be stated here, number of isolates isolated per sample, in particular for Campylobacter spp..

(c): Antimicrobials included, Cut-off values

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