Instructions Dp25 Microscope Digital Camera

INSTRUCTIONS DP25 MICROSCOPE DIGITAL CAMERA

This instruction manual is for the OLYMPUS DP25 Microscope Digital Camera. To ensure safety, obtain optimum performance and familiarize yourself fully with the use of this camera, we recommend that you study this manual thoroughly before operating the camera. Retain this manual in an easily accessible place near a system for future reference. ⎯⎯This publication is printed on 100% recycled paper.⎯⎯ Any copyrights relating to this manual shall belong to OLYMPUS CORPORATION. We at OLYMPUS CORPORATION have tried to make the information contained in this manual as accurate and reliable as possible. Nevertheless, OLYMPUS CORPORATION disclaims any warranty of any kind, whether expressed or implied, as to any matter whatsoever relating to this manual, including without limitation the merchantability or fitness for any particular purpose. OLYMPUS CORPORATION will from time to time revise the software described in this manual and reserves the right to make such changes without obligation to notify the purchaser. In no event shall OLYMPUS CORPORATION be liable for any indirect, special, incidental, or consequential damages arising out of purchase or use of this manual or the information contained herein.

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Printed in Germany Contents

Important information...... 3 About the contents of this manual ...... 3 Warning ...... 3 Maintenance and Storage ...... 4 Conformity of the System ...... 4 Restrictions ...... 4 Operating Environment ...... 5 System Chart...... 6 Nomenclature...... 7 Installation and operation of the DP25 for TWAIN...... 8 System Requirements ...... 8 Installation of the camera ...... 9 Using the TWAIN Interface ...... 20 Acquiring images ...... 21 Adjusting the exposure time ...... 21 Carrying out a white balance ...... 24 Selecting the resolution ...... 24 Trimming the image (subarray) ...... 25 Focusing the sample ...... 25 Zooming the image ...... 26 Shift Metering Regions ...... 26 Settings ...... 26 Installation of the DP25 for the DP2-BSW software..... 35 System Requirements ...... 35 Installation of the camera ...... 36 Acquiring your first images ...... 47 Special camera functions ...... 48 Contents

Specifications...... 50 Help with Problems...... 52 Your image doesn't look as good as it should ...... 52 "Live" and "Snap" are not active ...... 54 When connecting the camera to the Firewire interface before installing the DP25 TWAIN driver software or the DP2-BSW software ...... 54 Declarations of Conformity...... 61 Important information Important information

About the contents of this manual The DP25 is a high resolution CCD color camera for large scale applications in the brightfield microscopy field. Impressive features of this camera are its superior color quality and rendition. Not to mention the DP25 camera's compact design and ease of use. The DP25 is a camera that you can use both with the DP2-BSW software of OLYMPUS, as well as with any other software you wish, that supports TWAIN. This manual is made up of two parts. Should you work with the DP2-BSW software, you will only need to read the part "Installation of the DP25 for the DP2-BSW software", beginning on page 35. Should you wish to use the camera without the DP2-BSW software, read only the part "Installation and operation of the DP25 for TWAIN", beginning on page 8. Warning Warning Please install the DP 25 TWAIN driver software (TWAIN Interface) or the software with DP25 camera driver (especially DP2-BSW) before connecting the camera to the FireWire interface. Warning Do not disassemble the camera housing! CCD image sensors are easily damaged by static discharge.

Warning To avoid damage to the cable and the risk of fire, make sure that the camera cables do not come into contact with the light source or other sources of heat.

Warning In case of a fire toxic gases can be given off!

Warning Arrange the cables in such a way that nobody can stumble or trip over them. 3 Warning Do not touch cover slip (over the CCD chip) with your hand or have any object come in contact with glass surface. Should dust stick to the cover slip, blow it off gently with an air blower. (For dust stuck due to static electricity, ionized air is recommended.) Warning Do not subject the camera to too much mechanical shock.

Warning If the FireWire cable is damaged or severed, you risk an electric shock if you come in direct contact with it. Shut off the hardware and change the FireWire cable immediately! Important information

Warning To prevent the microscope from toppling down, avoid using microscope attachments that may make the total height of the microscope above 1 m when they are attached. Operating • Do not expose to strong light (sunlight) for long periods. Environment • High temperatures (> 40°C) and humidity (> 80%) can influence the camera's capabilities. Avoid storage or usage under such conditions. Start up • The FireWire cable plug is not symmetrical. You must make sure that the cable plug is facing the same way as the camera socket when you plug the cable into the camera jack. • Do not disconnect the FireWire cable while the TWAIN Interface or the DP2-BSW software is running. Maintenance and Storage 1) To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it with gauze slightly moistened with commercially available absolute alcohol. Warning Since the absolute alcohol is highly flammable, it must be handled carefully. Be sure to keep it away from open flames or potential sources of electric sparks - for example, electrical equipment that is being switched on or off. Also remember to always use these chemicals only in a well-ventilated room.

2) Parts other than the glass components should be cleaned by wiping with a clean cloth. Do not use organic solvents to remove major stains. Use a soft cloth slightly moistened with a neutral detergent solution. 3) Do not disassemble any part of the camera as this could result in malfunction or reduced performance. Conformity of the System

Restrictions 1) The recommended camera adapter is the U-TV0.5XC-3, U-TV0.63XC, MVX- 4 TV0.63XC or the combination U-TV1X-2 + U-CMAD3. The U-TV0.5XC should not be used because it deteriorates the image flatness. A camera adapter with a magnification below 0.5X cannot be used because part of image will be cut off. 2) When the D25 is connected to the rear port of the U-DPT or U-MPH, the peripheral part of the recorded image may be deteriorated due to the optical performance of the U-DPT or U-MPH. 3) When the U-TV0.5XC-2 or U-TV0.5XC-3 is used, using two or more intermediate attachments* may obscure or cut off the peripheral part of the field of view or may make flare noticeable. Important information

* Example of two intermediate attachments: BX microscope + Vertical illumina- tor + Intermediate attachment with a length equivalent to the U-CA 4) Under fluorescent ring illumination or other AC-driven illumination such as a phase control light intensity adjusting illumination system, the following phenomena may be observed when the light intensity is increased and exposure time is decreased: • Flickering of the displayed image. • Instability in exposure. However, provided that the brightness can be adjusted using the light intensity control knob is possible or ND filters, the above phenomena may be attenuated by adjusting the brightness so that the exposure time exceeds 20 ms. For details on the microscope models using AC-driven illumination, consult Olympus. 5) Non-Olympus microscopes and commercially available C-mount lenses can be used provided that they match a CCD with a size of no less than 2/3 inch and the lens projection length from the C-mount body attaching section is no more than 6 mm. However, problems due to optical adaptability, such as shading, may be observed. 6) When the specimen has a low contrast (near transparent) or high reflectance (mirror status) and the aperture iris diaphragm is stopped down near the smallest aperture, spot flare may be noticeable. 7) When the edge of a non-transmitting object is observed under the STM6 transmitted illumination, flare may be noticeable due to the difference in brightness between the transmitted sections (over-exposure) and non-transmitting section (underexposure). To reduce the flare, set a lower exposure using the exposure correction function or setting the exposure manually. 8) When a low-power objective (below 4X) is used, the peripheral part of the field of view may be obscured. In this case, use an ultralow-magnification condenser (U-ULC-2). 9) This system cannot photograph dark specimens in reflected light fluorescence or darkfield observation (a specimen that required exposure of 1/2 sec. or more when the ISO is equivalent to 100). 10) When using a stereo microscope at low zoom magnification, insufficient peripheral illumination may lead to the image brightness shading and/or vignetting. 5 11) Do not shut the aperture iris and field iris diaphragms of the microscope more than required.

Operating Environment Temperature: 5° to 40° C. Humidity: 5% to 80% (without condensation). System Chart System Chart Please note: Microscopes that are not listet in the system chart may also be applicable. For details, please consult Olympus.

DP25 Monitor

Camera Head IEEE 1394a IEEE 1394a PC FireWire Cable FireWire PCI Board DP25 DP2-BSW TWAIN Driver Basic Software

GX-TV0.5XC C-Mount U-CMAD3 Camera Adapter C-Mount Adapter GX-TV0.7XC C-Mount Camera Adapter

MVX-TV0.63XC U-TV1X-2 U-TV0.5XC-3 U-TV0.63XC C-Mount Camera C-Mount C-Mount Camera Adapter Adapter Camera Adapter Camera Adapter MVX-TV1 XC C-Mount Camera Adapter

MVX10 SZX16/SZX10 GX71/GX51 BX61/BX51/BX41 SZX12/SZX9 Inverted

6 STM6 IX81/IX71/IX51 CX41/CX31 SZX7/SZ61 Metallurgical Measuring Inverted MX80/MX50/MX40 Stereo Microscope Microscope Microsope MX61L/MX61/MX51 Microscope Microscope Nomenclature Nomenclature

Camera Head

Connector

C-Mount thread

FireWire PCI Board (PCI standard half size)

IEEE 1394a Connectors FireWire PCI Cable

DP25 TWAIN driver (CD-ROM) 7 Installation and operation of the DP25 for TWAIN Installation and operation of the DP25 for TWAIN With the TWAIN Interface you control the acquisition of images. You can open the TWAIN Interface from every application program that supports TWAIN. In this way, you can easily acquire, look at, and edit images, with a software that you are familiar with, for example, with Microsoft Word®. Warning Please install the TWAIN driver software (TWAIN Interface) before connecting the camera to the FireWire interface.

System Requirements To run your DP25 you have to use the Microsoft Windows XP operating system.To make proper use of your DP25 via TWAIN, the following system requirements for your PC have to be observed: Hard- / Software Required Recommended CPU Pentium 4, 2.4 GHz or higher Pentium D930, 3.0 GHz, Dual Core Pentium M, 1.4 GHz or higher Pentium M, 2.6 GHz

Hard Disk Storage 5 GB or more 20 GB

Memory 512 MB or more 1024 MB

Operating system Microsoft Windows XP Pro SP 2

Ports IEEE 1394a (Firewire™) 6 pin type Required power supply capacity : 12 V, 0.2 A

Application program Application program that supports TWAIN 8 Installation and operation of the DP25 for TWAIN

Installation of the camera PLEASE NOTE Please read this chapter carefully before you begin the installation. If you wish to run your camera with TWAIN, you will need to complete the following steps.

Check if a FireWire interface is available on your PC.

If necessary, install a FireWire PCI board. (The FireWire PCI board is included in your camera package.)

Install the TWAIN Interface.

Mount the camera on your microscope and connect it to your PC. Select the TWAIN driver.

Start the application program that supports TWAIN. 9 Installation and operation of the DP25 for TWAIN

Checking if a FireWire interface is available on your PC If your PC already has a FireWire interface onboard or a separate FireWire PCI board installed, you do not need to install the board provided with the camera. If you are not sure whether a FireWire interface is available, open the Microsoft Win- dows Device Manager via Start > Control Panel > System > System Properties > Hardware > Device Manager... . Check that the IEEE 1394 Bus host controllers entry is there.

Installing the FireWire PCI board IEEE 1394a

10 Warning When you install the FireWire PCI board, sharp edges may cut your fingers, so take extra care.

1) Turn off the PC and disconnect the power supply. 2) Remove the cover from the PC. 3) One PCI slot is required for the FireWire board. Insert the board into the slot. Installation and operation of the DP25 for TWAIN

FireWire PCI board installed

4) Replace the cover on the PC. 5) Restart your PC.

Installing the TWAIN Interface Warning Please install the TWAIN driver software (TWAIN Interface) before connecting the camera to the FireWire interface.

1) Put the CD-ROM that has been supplied into your CD-ROM drive. " The installation program will start automatically - unless you have deactiva- ted the autorun function. If so, please start the setup.exe file manually. 11 Installation and operation of the DP25 for TWAIN

The TWAIN installation will be prepared.

A dialog box with the license text will appear automatically. 12

2) Read the license agreement carefully. If you agree, select the Yes ... option and click the Next > button. Installation and operation of the DP25 for TWAIN

The Customer Informa- tion dialog box will open.

3) In the Customer Information dialog box, enter your name and your company name.The name, that has been used for the registration of the operating system, will be suggested. In this dialog box, you also specify the users who will be allowed to use the TWAIN interface on your PC. 4) Click the Next > button. The Choose Destination Location dialog box will open. 13 Installation and operation of the DP25 for TWAIN

5) If you want the change the directory on which the TWAIN interface is to be installed, click the Browse... button. Select the desired directory. You should only change the suggested directory if there is an important reason for it. 6) Click the Next > button. The Start Copying Files dialog box will open.

7) To start the actual installation click the Next > button. The installation of the TWAIN Interface has been completed. Click the Finish button to exit the Setup Wizard dialog box. 14

8) Click the Finish button to finalize the installation. " The TWAIN Interface has now been installed. Installation and operation of the DP25 for TWAIN

Mounting the camera and selecting the camera driver Warning Please install the TWAIN driver software (TWAIN Interface) before connecting the camera to the FireWire interface.

Selecting the camera driver 4) Plug either end of the FireWire cable into the camera connector. PLEASE NOTE The FireWire cable plug is not symmetrical. You must make sure that the cable plug is facing the same way as the camera socket when you plug the cable into the camera jack. The camera chip may be ruined otherwise. Make sure the cable plug is facing the right way before you plug it into the camera socket.

5) Plug the other end into one of the free connector sockets on your PC's FireWire 15 PCI board. " There are two or more sockets depending on the FireWire board. You can use any of them. " The Found New Hardware Wizard dialog will automatically appear. 6) You then answer the question Can Windows connect to Windows Update to search for software?, with No, not this time. Then click the Next> button. Installation and operation of the DP25 for TWAIN

You then answer the question Can Windows connect to Windows Update to search for software?, with No, not this time.

" A new dialog box entitled Found New Hardware Wizard will open. 7) Select the Install from a list or specific location (Advanced) option, and click the Next > button. In this dialog box, select the Install from a list or specific location (Advanced) option. 16

" A new dialog box entitled Found New Hardware Wizard will open. Installation and operation of the DP25 for TWAIN

8) Select the Don’t search. I will choose the driver to install option, and click the Next > button. In this dialog box, select the Don’t search. I will choose the driver to install option.

" A new dialog box entitled Found New Hardware Wizard will open. It shows the correct OLYMPUS CORPORATION FireWire Camera 2.0.26 (SoftHard) driver. The correct FireWire camera driver is displayed in the Model list of this dialog box. 17 Installation and operation of the DP25 for TWAIN

9) Select the OLYMPUS CORPORATION FireWire Camera 2.0.26 (SoftHard) entry in the list. 10) Click the Next > button. " The next Found New Hardware Wizard dialog box will be opened in the background, displaying the message to wait while the Wizard installs the software. The Hardware Installation dialog box shown here may pop up.

11) As the information given in this dialog box is not important for the installation, simply click the Continue Anyway button. " The Hardware Installation dialog box closes. " The Found New Hardware Wizard dialog box which had been in the background, comes to the foreground. 18 Installation and operation of the DP25 for TWAIN

12) When the camera driver for the DP25 has been selected, the following dialog box will open. 19

13) Click the Finish button to end the Found New Hardware Wizard. " The DP25 is now ready for use. Installation and operation of the DP25 for TWAIN

Starting the application program Start the application program of your choice that supports TWAIN. " The DP25 is now registered as a TWAIN device and can be selected. Using the TWAIN Interface Select the DP25 as a TWAIN source, and start the image acquisition. The exact name of the commands, used to select the TWAIN source and start the image acquisition, depends on the application program you are using. For more detailed information please refer to the user’s manual of the application program you are using. " The Olympus Twain window will open. This is the TWAIN Interface. " The TWAIN Interface's image window displays the live-image.

Image Window Camera Control 20

The TWAIN Interface's image window displays the live-image. Installation and operation of the DP25 for TWAIN

Acquiring images Click the Snap button. " The image will be transferred to the software application from which you started TWAIN. You can edit or save it there. On the right-hand side Perform a white balance, of the Olympus Twain Use Shift Metering Regions window, you will find all of the controls you need Close TWAIN Interface / to optimally adjust Acquire Image images.

Select exposure time mode and Set exposure time

Select resolution (and Binning)

Use focus indicator

Trim the image (subarray)

Open the Camera Settings dialog box

Adjusting the exposure time 21 An automatic exposure time mode, which will already produce excellent results for a great variety of acquisitions, is preset. Depending on the properties of your sample, however, it can become necessary to switch to the manual exposure time mode (e.g., samples with a strongly reflecting surface). If you want to change the exposure time mode, click the option you want in the Expo- sure group (either Manual or Automatic). Installation and operation of the DP25 for TWAIN

Manual exposure In the manual exposure time mode you can enter the exposure time yourself. This time mode mode can be recommended, for example, when you want to make a variety of different acquisitions of a sample to be able to compare their brightness.

This is how you set the exposure time manually You have the following options: • Use the slide controls. • Click the plus and minus buttons. • Click the arrow buttons at the right-hand end of the edit field. • Enter an exposure time in the edit field, then press the [Enter] key. In the manual exposure time mode you can select the exposure time yourself.

" The exposure time will be adjusted accordingly. If you wish, the TWAIN Interface can supply you with a benchmark, with the help of which you will be able to set a suitable exposure time. To obtain this, click the One time Auto Exposure button. " The value set will be that which the automatic exposure time mode currently supplies. You can then adjust this value as you wish. Automatic In the automatic exposure time mode, the TWAIN Interface automatically defines the exposure time exposure time for each single acquisition, so that the brightest part of the image mode always remains the same. This way you can always see the optimal image. In automatic exposure time mode the exposure time is constantly checked and updated automatically. 22 This is how you set the exposure time automatically If it is not already selected, click the Automatic option. You will find this option in the Exposure group. " The TWAIN Interface will then calculate the exposure time automatically. PLEASE NOTE In the camera settings, maximum exposure times that limit the upwards search for the correct exposure times, are defined. You can see these values in the Camera Settings dialog box, and can also change them there. Click the Settings button in the camera control. Activate the DP25 > Exposure option. Then you will see the Maxi- mum exposure time group. Installation and operation of the DP25 for TWAIN

This is how you configure the automatic exposure time Click the Lock Current Exposure Time button to freeze the current exposure time. " Even when you move to a completely different place on your sample which exhibits a different brightness, the exposure time will not be adjusted accordingly. " The Lock Current Exposure Time button will remain active (also for later images), until you switch it off again. Region If you want to, you can determine that the exposure time will only be measured on a part of the image, you do this in the Region field. Select an entry from the picklist.

" If you have selected a spot, you have to define the Region of Interest (ROI) on which the exposure time is to be measured, in the image. " Should you want to position the spot in the center of the image, you should use the Center Spot on Image button for this. " Select the Full Image entry, if the exposure time is to be measured on the whole image. Adjustment If you want to, you can select a value from the Adjustment picklist. With this value you can correct the automatic exposure time that has been calculated by the TWAIN Interface. " To correct the automatic exposure time, switch to the live-mode and select a value from the Adjustment list. The largest possible correction value is 4, the smallest -4. " Should your images be overexposed or appear to be too bright, when you

use the automatic exposure time, select a negative correction value. 23 Should your images be underexposed when you use the automatic exposure time, select a positive correction value. Select the value 0, if there is no need to correct the automatic exposure time. " Test the effect of the exposure time correction in the live-image. Installation and operation of the DP25 for TWAIN

Carrying out a white balance Before an acquisition you must carry out a white balance if the colors are not correctly reproduced. When using white balance, the individual color channels are scaled in such a way that the white or neutral gray area of the image displayed on the monitor is displayed correctly as white or gray. Carrying out a white balance 1) Click the White Balance on ROI button. You will find this button above the Close and Snap buttons. 2) In the image window, draw a frame with the left mouse button depressed. The area within this frame is set to white. Change the position and the size of the frame in such a way that the image area covered by the frame is well suited for performing the white balance. 3) When you are satisfied with the frame's position, click the right mouse button and select Confirm Input. Alternatively you can doubleclick in the frame. " The settings for the individual color channels will be optimized in the set area. You can see the results immediately in the image. Selecting the resolution Your DP25 can acquire images with a variety of different resolutions. The following resolutions are available: 2560 x 1920, 1280 x 960 (binning), 854 x 640 (binning) and 640 x 480 (binning). The following resolutions are available: 2560x1920, 1280x960, 854x640 and 640x480.

By default, the "2560x1920" option is always preset. When the highest resolution has been set, all of the data the camera has acquired will be read out, and therefore the image will contain the most information.

24 The other resolutions require lower exposure times. Changing the To alter the resolution, select the value you want from the Snap Resolution picklist. resolution Installation and operation of the DP25 for TWAIN

Trimming the image (subarray) If you are only interested in a part of the image on display, you can determine that only the data pertaining to this part of the image will be read out of the camera. You can define which part that is in the image. With a subarray you limit the amount of data that is exported from the camera to the TWAIN Interface. You trim the image already before the acquisition. This will increase the frame rate, and reduce the file size of the acquired images.

This is how you set up a subarray 1) Define which part of the image you are interested in. To do so, click the Set ROI for Subarray button. " The last previously defined ROI (Region of Interest) will be displayed. 2) Move the ROI to the place in the image that interests you. To do this, click in the ROI, then with the left mouse button depressed, drag it to the place you want it. 3) Then adjust its size. To do this, drag the handles of the ROI. Confirm the ROI either by doubleclicking in it, or by selecting the Confirm Input command in the context menu. Rightclick to open the context menu. " By doing so, you switch the subarray on. The image will then only show the image segment you have defined.

This is how you switch off a subarray Click the Toggle Subarray button to switch the subarray method on or off.

Focusing the sample Focus Indicator Use the Focus Indicator to bring the sample into focus. Unlike the human eye, the Focus Indicator calculates the sharpness of the image on a mathematical basis. The Focus Indicator enables you to control the focus settings. To begin with, you define a rectangular area of the image that is to be analyzed for this calculation. The maximum contrast serves as a monitor for the sharpness in this area. The Focus Indicator has a display that indicates the relative degree of sharpness by means of two superimposed bars. One of these bars is red, the other blue. The red

bar shows the greatest sharpness that has currently been achieved. The blue bar 25 shows the sharpness that is being achieved with the current focusing.

This is how you bring the sample into focus by using the Focus Indicator 1) Go to the place in the sample that interests you. 2) Click the Toggle Focus Indicator button to switch the Focus Indicator on. " The sharpness monitor in the Focus Indicator will appear. " A yellow rectangle will appear in the image. Only the pixels that are within this rectangle will be taken into account for the measurement of the sharpness of your image. Installation and operation of the DP25 for TWAIN

3) Change the position and size of the rectangle, so that an area of the sample that especially interests you will be measured. Please, make sure that the area within the yellow rectangle shows a high contrast portion of the sample. 4) Turn the precision focusing knob on your microscope to change the focusing. While doing this, watch the display in the Focus Indicator. " The red bar shows the greatest sharpness that has currently been achieved. " The blue bar shows the sharpness that is being achieved with the current focusing. 5) Change the focusing on your microscope until the display is as completely blue as possible. 6) Click the Reset Focus Indicator button, to reset the sharpness monitor's bars at the relative value of 75% for a better visualization.

PLEASE NOTE A binning that has been set does not influence the functionality of the Focus Indicator.

Zooming the image The image located in the TWAIN interface’s image window can be zoomed in or out. Zooming in the If your mouse is equipped with a mouse wheel, turn the mouse wheel backwards to image zoom in the image. Or, press the [Crtl] key and then click the left mouse button. Zooming out the If your mouse is equipped with a mouse wheel, turn the mouse wheel forwards to image zoom out the image. Or, press the [Crtl + Alt] keys simultaneously and then click the left mouse button.

Shift Metering Regions For two of the DP25 functions you will need to define a rectangular image area. One of these functions is the Spot mode, used for the calculation of the automatic exposure time, the other is the Focus Indicator. The areas you have defined can, at any time, be moved or changed in size. Release the Shift Metering Regions button when you want to fix the position of these areas on an image.

Settings 26 If you want to change the camera settings, click the Settings... button. The dialog box's tree view offers you access to the General, Color, Adjustment, Exposure and Information dialog boxes. Select an entry in the tree view to load the respective dialog box. Click the Default button to return the settings in the active dialog box to their default values. The current settings will then be lost. Confirm your settings with OK. Installation and operation of the DP25 for TWAIN

General Use this dialog box in the Camera Settings to make a variety of general camera settings. You can, for example, mirror the camera image, or select a pseudo color table. The General dialog box

Mirror Select the Horizontal check box to mirror the image horizontally during acquisition. Select the Vertical check box to mirror the image vertically during acquisition. The two forms of mirroring can be combined with each other. Image Type Select the default option "24 bit RGB Color", to acquire images of the "24-bit true- color" type. Select the "8 bit Gray Scale" option to acquire images of the "8-bit gray-value" type. Pseudo Color You can use pseudo color tables to have images displayed in color on your monitor. By default, when you acquire images, no pseudo color tables will be used. In this case, the Pseudo Color > Off option is selected. Select the Use high/low pseudo color table option to have all of the pixels with a low intensity value displayed in blue, and all of those with a high intensity value displayed 27 in red. When you acquire this image, it will show all of the pixels in their actual color. With this pseudo color table you can immediately see if the image is well illuminated. When there are both blue and red pixels in the image, the camera's complete dynamic range will be in use. If too many blue pixels can be seen, the image is underexposed. If too many red pixels can be seen, the image is overexposed. Installation and operation of the DP25 for TWAIN

Color Use this dialog box in the Camera Settings to change the camera's color settings manually. In this way you can change the Parameters Gain, Offset and Saturation individually for each color channel. To do this, move their slide controls. R, G and B stand for the Red, Green and Blue color channels. The Color dialog box

Gain With the Gain (White Balance) value, you can weight the individual color channels (White Balance) separately. Increase, e.g. the R value, to increase the amount of red in the image. Saturation Increase the Saturation value, if the color is too pale, and you want a better color saturation. 28 Installation and operation of the DP25 for TWAIN

Adjustment Use this dialog box in the Camera Settings to change the camera's contrast settings manually. The Adjustment dialog box

You will change the settings for all color channels simultaneously. If you want to accentuate or tone down one particular color channel, use the functions in the Color dialog box. Gamma Gamma increases, respectively lowers the image contrast, subject to the intensity I of a pixel, according to the following equation. 1 ⁄ γ I0 = I

with I = intensity value of the destination image, I0= the intensity value of the source image. The values for Gamma vary between 0.2 and 5. Values smaller than 1 make the image altogether darker. At the same time, the contrast in bright areas of the image will be increased. Values greater than 1 make the image altogether brighter. At the same time, the contrast in dark areas of the image will be increased. 29 Sharpness Use the sharpen filter to increase or decrease the sharpness of the image by edge enhancement. You change the strength of the sharpen filter by moving its slide control. Values ranging from -30 to 30 are possible, whereas the default value is 0. The image gains a smoother appearance when you use negative values and a sharper one when you use positive values. Raising sharpness will accentuate edges, but also increases image noise. Entering a value of 0 will result in the sharpen filter having no effect at all. Installation and operation of the DP25 for TWAIN

Gain The intensity value will be multiplied by the Gain value. The image will then become altogether brighter. Contrast Move the Contrast slide to change the contrast values of the image. Values smaller than 1 lower the image contrast. Values greater than 1 enhance the image contrast. When changing the image contrast the brightest part of the image always remains the same. After changing the image contrast it may be that dark or bright image areas are not displayed optimally anymore. In this case use the Brightness slider to make the whole image brighter or darker. Brightness Use the Brightness slider, if the whole image is too bright or too dark. Values smaller than 0 lower the image brightness. Values greater than 0 increase the image brightness.

Shading correction In the Shading Correction group you can correct two different shadings. First of all, the shading correction corrects the effect of an inhomogeneous illumination (Illumination correction). Additionally, the shading correction corrects the different sensitivity of individual pixels on the CCD chip (Noise correction). Select the Dark option to activate the Noise Correction. Select the Flatfield option to activate both the Illumination Correction and the Noise Correction. Select the Off option to disable the a shading correction. Click the Edit... button to check whether correction images have already been acquired or not. If correction images exist, you will find them listed in this dialog box. In the Image Type column the correction images used for the Noise Correction are indicated by the Dark entry, the correction images used for the Illumination Correc- tion by the Flatfield entry. In this dialog box check, whether there are correction images of the Dark or Flatfield type, or not. 30

Definition Every optical system with a camera and microscope creates, even when the devices "Shading" have been set up very carefully set up, image inhomogeneities in the illumination of the sample. These are called shading. Installation and operation of the DP25 for TWAIN

The left illustration shows an image where the online shading correction has not been activated. In compari- son to the image on the right, one can notice the inhomogoneities in the illumination.

For a shading correction, the shading will first be determined in order to be able to make corrections to the acquired image. To determine which shading there is in the image, two types of correction images will be acquired: Dark correction The Dark correction image is an acquisition during which no light falls on the camera. image Here, pixels with different brightness resulting from noise or different sensitivity of individual pixels on the camera chip can be corrected by the correction image. The Dark correction image is characteristic for each camera and need only be acquired once. It is valid for all objectives. Flatfield correction On the Flatfield correction image the illumination of the complete optical system with- image out a sample will be shown. An individual correction image must be made for every objective and for every resolution of your camera. PLEASE NOTE You will need to acquire new illumination correction images every time you make changes in your optical system. For example, when you clean your microscope, change the objectives or change the camera.

Acquiring images for the shading correction 1) Open the Camera Settings > Adjustment dialog box. Click the Calibrate... button. " The Shading Correction wizard will open. 31 Installation and operation of the DP25 for TWAIN

The wizard leads you step by step through the calibration of the shading correction.

2) If you have already acquired a Dark correction image, the Skip recording dark correction image check box will be automatically selected. Clear the check box, if you want to acquire a new Dark correction image. Click the Next > button. The Dark correction image is an acquisition during which no light falls on the camera. This image is subtracted from the acquired image.

32 3) Then follow the instructions given you by the software wizard. Close the light path selector knob on your microscope, so that no light can fall on the camera. Then click Next. " The Dark correction image will be acquired. " The next dialog box of the wizard will open. Installation and operation of the DP25 for TWAIN

On the Flatfield correction images the illumination of the complete optical system without a sample will be shown.

PLEASE NOTE The Flatfield correction image depends on the objective being used. Correspond- ingly, an individual correction image must be made for every objective. If you want to use another objective for acquiring images, you will have to acquire a new Flatfield correction image. 4) Move to a position where is no sample and no scratches, and defocus. 5) Click the Next button and acquire the correction images for the currently selected objective. " The TWAIN interface automatically acquires the correction images for all camera resolutions. 33

6) Click the Finish button to finalize the calibration and return to the Camera Set- tings > Adjustment dialog box. Installation and operation of the DP25 for TWAIN

Exposure Use this dialog box in the Camera Settings to set the maximum possible exposure times for the image acquisition. Set an upwards limit to the maximum possible exposure time, to make sure that the live-image is in any case quick enough to be able to focus comfortably (also see "Adjusting the exposure time" on page 21). The Exposure dialog box

Information Use this dialog box in the Camera Settings to view information about your DP25 camera. 34 Installation of the DP25 for the DP2-BSW software Installation of the DP25 for the DP2-BSW software

System Requirements To run your DP25 you have to use the Microsoft Windows XP operating system.To make proper use of your DP25, the following system requirements for your PC and the DP2-BSW software have to be observed: Hard- / Software Required Recommended CPU Pentium 4, 2.4 GHz or higher Pentium D930, 3.0 GHz, Pentium M, 1.4 GHz or higher Dual Core

Pentium M, 2.6 GHz

Hard Disk Storage 5 GB or more 20 GB

Memory 512 MB or more 1024 MB

Operating system Microsoft Windows XP Pro SP 2

Ports IEEE 1394a (Firewire™) 6 pin type Required power supply capacity 12 V, 0.2 A

Image analysis DP2-BSW application 35 Installation of the DP25 for the DP2-BSW software

Installation of the camera In order to avoid problems, you will have to follow the steps of the procedure in the order given here.

Check if a FireWire interface is available on your PC.

If necessary, install a FireWire PCI board. (The FireWire PCI board is included in your camera package.)

Install the DP2-BSW program on your PC. (The camera driver will also be automatically installed.)

Mount the camera on your microscope and connect it to your PC.

Select the camera driver.

Start the DP2-BSW software.

36 Checking if a FireWire interface is available on your PC If your PC already has a FireWire interface onboard or a separate FireWire PCI board installed, you do not need to install the board provided with the camera. If you are not sure whether a FireWire interface is available, open the Microsoft Win- dows Device Manager via Start > Control Panel > System > System Properties > Hardware > Device Manager... . Check that the IEEE 1394 Bus host controllers entry is there. Installation of the DP25 for the DP2-BSW software

Installing the FireWire PCI board IEEE 1394a Warning When you install the FireWire PCI board, sharp edges may cut your fingers, so take extra care.

1) Turn off the PC and disconnect the power supply. 2) Remove the cover from the PC. 3) One PCI slot is required for the FireWire board. Insert the board into the slot. 37 Installation of the DP25 for the DP2-BSW software

FireWire PCI board installed

4) Replace the cover on the PC. 5) Restart your PC.

Installing the DP2-BSW software Warning Please install the DP2-BSW software before connecting the camera to the FireWire interface. Should the DP25 have already been connected to the PC before the DP2-BSW software was installed, Microsoft Windows XP will have installed its own driver, which will prevent the installation of the correct driver. Please note that this does not apply to the DP20! Should you decide to use the DP20 instead of the DP25, the PC will not automatically install the camera driver. When using the DP20 with the DP2-BSW software, you will need to manually install the camera driver from the CD-ROM. You can install the DP2-BSW software on your PC even if you have already installed another software package from Olympus Soft Imaging Solutions on it. Insert the software protection key (dongle) into a USB port of your PC.

38 1) Installation of the DP25 for the DP2-BSW software

Your DP2-BSW software is protected by a dongle. Insert it into a USB port of your PC.

2) Turn on your PC and, if necessary, start up the operating system. " The Found New Hardware dialog box will open. The operating system will then try to install a driver for the dongle. When you have plugged in the dongle, this dialog box will appear. 39

3) Since the correct dongle driver can only be installed during the installation of the image analysis program, you will then need to click the Cancel button. " The Found New Hardware dialog box will close. During the installation of your image analysis program, the correct dongle driver will be automatically installed. 4) Close any and all application programs. 5) Put the installation CD-ROM into your CD-ROM drive. Installation of the DP25 for the DP2-BSW software

" The installation program will open automatically - unless you have deactiva- ted the auto run function. If so, start the setup.exe file via Microsoft Windows Explorer. 6) To make the installation, simply follow the instructions of the installation wizard, and each time you have made a necessary entry, click the Next > button to continue. In the Image Source Selection dialog box, select Olympus from the Manufacturer/Image Source list and the DP25 entry located in the Device list.

The installation of the DP2-BSW software is complete. Click the Finish button to exit the InstallShield Wizard Complete dialog box. 40 Installation of the DP25 for the DP2-BSW software

Mounting the camera Warning Please install the DP2-BSW software before connecting the camera to the FireWire interface.

Mounting the camera 1) Remove the protective cap from the DP25. 2) Screw the camera adapter securely to the DP25 camera. This should be done with the CCD chip side of the camera facing down, to minimize the chance of dust landing on its surface. 3) Install the camera and the camera adapter onto the microscope's camera port. You may need to align the orientation of the camera with that of the microscope stage, and also match the focus of the camera image with that of the eyepiece image. This alignment can be made later when you are able to obtain an image on the screen. 4) Plug either end of the FireWire cable into the camera connector. PLEASE NOTE 5) The FireWire cable plug is not symmetrical. You must make sure that the cable plug is facing the same way as the camera socket when you plug the cable into the camera jack. The camera chip may be ruined otherwise. Make sure the cable plug is facing the right way before you plug it into the camera socket.

6) Plug the other end into one of the free connector sockets on your PC's FireWire PCI board. There are two or more sockets depending on the FireWire board.

You can use any of them. 41 Installation of the DP25 for the DP2-BSW software

Selecting the camera driver Prerequisite You have installed the DP2-BSW software and then connected the DP25 camera to the FireWire slot. " The Found New Hardware Wizard dialog will automatically appear. 1) You then answer the question Can Windows connect to Windows Update to search for software?, with No, not this time. Then click the Next> button. You then answer the question Can Windows connect to Windows Update to search for software?, with No, not this time.

" A new dialog box entitled Found New Hardware Wizard will open. 2) Select the Install from a list or specific location (Advanced) option, and click the Next > button. 42 Installation of the DP25 for the DP2-BSW software

In this dialog box, select the Install from a list or specific location (Advanced) option.

" A new dialog box entitled Found New Hardware Wizard will open. 3) Select the Don’t search. I will choose the driver to install option, and click the Next > button. In this dialog box, select the Don’t search. I will choose the driver to install option. 43 Installation of the DP25 for the DP2-BSW software

4) Select the OLYMPUS CORPORATION FireWire Camera 2.0.26 (SoftHard) entry in the list. If this entry is missing, click the Cancel button. Repeat the installation of the DP2-BSW software including the selection of the DP25 camera. 5) Click the Next > button. " The next Found New Hardware Wizard dialog box will be opened in the background, displaying the message to wait while the Wizard installs the software. 44 Installation of the DP25 for the DP2-BSW software

The Hardware Installation dialog box shown here may pop up.

6) As the information given in this dialog box is not important for the installation, simply click the Continue Anyway button. " The Hardware Installation dialog box closes. " The Found New Hardware Wizard dialog box which had been in the background, comes to the foreground. 45

7) After completing the installation of the driver for the DP25 camera, the following dialog box is opened. Installation of the DP25 for the DP2-BSW software

8) Click the Finish button to end the Found New Hardware Wizard. " The DP25 is now ready for use.

Starting the DP2-BSW software 46 Installation of the DP25 for the DP2-BSW software

Acquiring your first images Prerequisites You have successfully installed your system (DP2-BSW software, camera, and camera driver). You would like to acquire your first images. 1) Start the DP2-BSW software. " You will automatically be in the Acquisition layout. This means that all of the software controls are optimally positioned on your monitor for acquiring images. " On the right-hand edge of your screen you will find the Camera Control tool window. There, the Live and Snap buttons are active. You can now acquire images and make changes in the camera settings. Live Click the Live button in the Camera Control tool window or use the Acquire > Live command. The acquired image is shown live in the document window. Use the live-mode for focussing or when arranging the camera or the sample. Snap Click the Snap button in the Camera Control tool window or use the Acquire > Snap command. Use the Snap mode, to stop the live-image or to acquire a snapshot. You can edit or save the image you have acquired. The way to make image acquisitions is described in detail in your DP2-BSW software manual. 47 Installation of the DP25 for the DP2-BSW software

Special camera functions In the following you will find a description of two controls that can be found in your DP2-BSW software's Camera Control tool window when you work with the DP25. You will only find these two controls when you work with the DP25. All of the other functions you will find described in detail in your DP2-BSW software's manual. The DP2-BSW program's Camera Control tool window. Here, only the two functions that are specific to the DP25, "Field Update" and "Trimming the image" will be described.

DP25 > Field Update

48 Trim the image (subarray) Installation of the DP25 for the DP2-BSW software

Trimming the image (subarray) If you are only interested in a part of the image on display, you can determine that only the data pertaining to this part of the image will be read out of the camera (Partial Readout). You can define which part that is in the live-image. With a subarray you limit the amount of data that is exported from the camera to your DP2-BSW system. You trim the image already before the acquisition. The frame rate of the live-image will then increase and the size of the file in which the acquired images are saved will be reduced.

Setting a subarray 1) First you define which part of the image you are interested in. To do so, click the Set ROI for Subarray button. " The mouse pointer will appear within the image. The last previously defined ROI (Region of Interest) will be displayed. 2) Move the ROI to the place in the image that interests you. To do this, click in the ROI, then with the left mouse button depressed, drag it to the place you want it. 3) Then adjust its size. To do this, drag the handles of the ROI. Confirm the ROI either by doubleclicking in it, or by selecting the Confirm Input command in the context menu. Rightclick to open the context menu. " By doing so, the subarray is switched on. The live-image will then only show the image segment you have defined. 4) Then determine whether the subarray is only to be employed for displaying the live-image, or also for the acquisition. If it is also to be employed for the acquired image, clear the Live only check box. " By default, the subarray will only be employed for the live-image, that is why the Live only check box is preselected. " The display will be updated.

Field Update The Field Update check box switches between a simple and an enhanced frame rate. The performance of the CPU in your PC determines the extend to which the frame rate can be enhanced. Select the Field Update check box to increase the frame rate. By default, the enhanced frame rate is set. This means that the live-image will be quickly updated, even if the image information changes quickly. However, this setting will require a 49 considerable amount of your CPU's performance. Clear the Field Update check box, if an enhanced frame rate is not especially important for you. This will avoid blocking more of your CPU's performance than necessary. Specifications Specifications

DP25 Specifications Item Specifications Camera Single-CCD color camera Image pickup device 2/3-inch color CCD Total pixels: 5.24 million pixels Effective pixels: 4.92 million pixels Pixel pitch: 3.4 µm (H) x 3.4 µm (V) Scanning method: Interline scanning Cooling Natural air cooling Recorded image sizes 2560 x 1920 (1 x 1) 1280 x 960 (2 x 2) 854 x 640 (3 x 3) 640 x 480 (4 x 4) Lens mount C-mount A/D 12 bits Exposure control* Exposure modes Auto Manual AE lock Available One time AE Available Exposure correction** Correction range: ± 4.0 EV. Steps: 1/3 (<2); 1/2 (>2) Metering area Full image Crosshair 30% Metering area is freely movable. 1% 0.1% Exposure time 200 µs to 16 s Binning 2 x 2, 3 x 3, 4 x 4 Live frame rate**,*** Field Update 2560 (H) x 1920 (V) (1 x 1): approximately 8 fps 854 (H) x 640 (V) (3 x 3): approximately 24 fps 640 (H) x 480 (V) (4 x 4): approximately 32 fps Frame Update 2560 (H) x 1920 (V) (1 x 1): approximately 4 fps 1280 (H) x 960 (V) (2 x 2): approximately 8 fps 50 854 (H) x 640 (V) (3 x 3): approximately 12 fps 640 (H) x 480 (V) (4 x 4): approximately 16 fps Color modes Color (24 bits) Gray scale ( 8 bits) White balance mode* White Balance on ROI Gain (White Balance)* Manual; Range: 1 to 10; Increment: 0.01 Offset (Black Balance)** Manual; Range: 0 to 100; Increment: 1 Saturation* Range: 0 to 100; Increment: 1 Specifications

Item Specifications Gamma* Range: 0.2 to 5; Increment: 0.001 Sharpness filter* Range: -30 to 30; Increment: 1 Gain* Range: 1 to 5; Increment: 0.01 Contrast* Range: 0.5 to 2; Increment: 0.01 Brightness* Range: -100 to 100; Increment: 1 Focus indicator* Contrast bar Interval recording** Recording time: 1 s to 9999 h; Fixed Interval: 1 s to 1000 h Image file formats** TIFF BMP JPEG JPEG 2000 PSD PNG PC Interface IEEE 1394a (FIrewire) 6 pin type Required power supply capacity for DP25: 12 V, 0.2 A Compatible OS Microsoft Windows® XP Professional SP 2 (not compatible with x64 Edition) Dimension and weight Camera head 86 mm (Ø) x 48 mm (H); approximately 350 g IEEE 1394a board 121 mm (W) x 132 mm (D) x 21.5 mm (H); approximately: 60 g IEEE 1394a cable 4.5 m length Power consumption 12 V, 0.2 A Operating environment Indoor use Ambient temperatures: 5 °C to 40 °C (41 °F to 104 °F) Relative humidity: 5% to 80% (without condensation) Supply voltage fluctuations by IEEE 1394a: ± 10% * These items can be set from the DP2-BSW V1.2 or TWAIN Interface V1.1 ** These items can be set from the DP2-BSW V1.2 *** The values of these items are variable depending on the exposure time setting and the PC operating 51 Help with Problems Help with Problems You can solve most of your image problems by changing the camera settings. To open the Camera Settings dialog box, click the Settings button located in the Camera Control tool window. By default, you will find the Camera Control tool window on the right-hand edge of your screen.

Your image doesn't look as good as it should

What should I do, when the live-image suddenly appears to be "frozen", and I receive an "Error making a snapshot" message? Check to see if the connector cable between the camera and the PC has been correctly plugged-in. Should the connector be loose or not plugged in correctly, correct this by plugging it firmly into the corresponding jack. Restart the DP2-BSW software. Should the problem still exist, contact your sales dealer.

What should I do if the image "quivers"? Quivering is caused by a frame rate that is too low. Select a lower resolution and/or a shorter exposure time. And increase the intensity.

What should I do when the image appears to be too dark? • Check on your microscope whether a diaphragm has been closed too much, or the beam splitter has been adjusted in such a way that no light can reach the camera. Should your image still be too dark, then please check the following points one after the other. • The exposure time is possibly too short. Select a longer exposure time! " In the manual exposure time mode: Click the Settings... button in the Camera Control tool window. In the Camera Settings dialog box select the Exposure entry from the tree view. In the Camera Settings > Exposure dialog box, check whether the maximum allowed exposure time has been reached. If necessary, increase this value. To do so, move the Manual slide control to the right. " In the automatic exposure time mode: You should check in the Camera Set- tings > Exposure dialog box, whether the maximum allowed exposure time has been reached. If necessary, increase the "Maximum exposure time" 52 value. To do so, move the Automatic slide control to the right. Alternatively, you can also enter a higher value directly in the text field. • It is possible that the exposure time correction has been set too low. In the Camera Settings > DP25 > Exposure dialog box, click the Exposure Correc- tion... button, and make sure that the exposure time correction does not lie below 0. Should this exposure time correction show a negative value, then please reset it to 0. Help with Problems

What should I do when the image appears to be too bright? • First of all, make sure that your microscope is correctly set up (diaphragm opening, beam splitter...). Should your image still be too bright, then please check the following points one after the other. • The exposure time is possibly too long. Select a shorter exposure time! " In the manual exposure time mode: In the camera control reduce the exposure time. " In the automatic exposure time mode: The lowest possible exposure time of the camera (200 µs) has possibly been reached. In the Camera Settings > Adjustment dialog box set the Gamma, Sharp- ness, Gain, Contrast, and Brightness values at their resp. default values. To do this, click the Default button. • In the Camera Settings > DP25 > Exposure dialog box, click the Exposure Correction... button, and make sure that the exposure time correction does not lie above 0. Set the value to 0.

What should I do when the image is monochrome? First of all, check which type of image has been set. Should the Settings > DP25 > General > Image type > 24 bit RGB color option not have been set, then set it. Should the image have been set as a "24 bit RGB color" type of image, but the image is still monochrome, carry out a white balance with an appropriate sample. Should your image show a red tinge, despite your having carried out a white balance, this is because your lamp's color temperature is too low. Increase the lamp voltage. As a next step, you should check the following settings on your PC and its monitor. Via Control Panel > Display properties > Settings > Color Quality, set your PC's color depth at a minimum value of 24 bits. Select, for example, the Highest (32 bit) option. Set your monitor's color temperature either via the monitor's menu navigation or via the operating system. This is how you set the color temperature via the operating system 1) Rightclick on the desktop. 2) Select the Properties option in the context menu. " The Display Properties dialog box will open.

3) Select the Settings tab. 53 4) Click the Advanced... button. " A further Display Properties dialog box will open. The appearance and functions of this dialog box depend on the graphics card that has been installed in your system, and on your monitor. 5) Look for a tab with either the name Color or Color Management. 6) Select a color temperature whose color impression as near as possibly matches that of the image in the eyepiece. Help with Problems

What should I do if the image is blurred and/or lacks contrast? Focus the sample visually on your microscope. Set the camera adapter so as to make the eye piece's focal plane identical to that of your camera. Take as much care as possible to ensure that your camera and your microscope optics are kept clean, and that the whole system is mechanically stable. Make sure that the condenser's aperture stop is not open too wide.

"Live" and "Snap" are not active

What should I do when the "Live" and "Snap" buttons are inactive? This chapter is only relevant, if you use the DP25 with the DP2-BSW software. Check to see if the connector cable between the camera and the PC has been correctly plugged-in. Should the connector be loose or not plugged in correctly, correct this by plugging it firmly into the corresponding jack. Restart the DP2-BSW software. Should the problem still exist, continue with the following section. In the Device Manager, check if there is an IEEE 1394 Bus host controllers entry there, and also that the OLYMPUS CORPORATION FireWire Camera 2.0.26 (Soft- Hard) entry is there under the Imaging devices branch. Open the Microsoft Device Manager via Start > Control Panel > System > System Properties > Hardware > Device Manager. Should these entries not be present, the driver will then have to be installed afterwards, independently from the installation CD-ROM (see "How can I reinstall the camera driver?" on page 54). Should the problem not have been solved even when the driver has subsequently been successfully installed, check whether an Optical Path has been entered in the Optical Path dialog box. You can open the Optical Path dialog box via the Acquire > Devices > Optical Path... command. Should no entry be displayed in the upper list in the Optical Path dialog box, set one up by using the New Wizard button. Please refer to the online help of the DP2-BSW software. There you will find a step by step instruction how to set up an Optical Path. Should the problem still exist, contact your sales dealer.

When connecting the camera to the Firewire interface before installing the DP25 TWAIN driver software or the DP2-BSW soft- 54 ware

How can I reinstall the camera driver? PLEASE NOTE Should the camera have already been connected to the PC before the TWAIN Inter- face or the DP2-BSW software was installed, Microsoft Windows XP will have installed its own driver, which will prevent the installation of the correct driver. Install- ing the DP2-BSW software afterwards or reinstalling the TWAIN interface will be of no help! The driver will have to be installed separately. Help with Problems

1) Open the Microsoft Device Manager via Start > Control Panel > System > System Properties > Hardware > Device Manager. To open the MS Device- Manager, first click Start then click Control Panel. Then click the Device Manager button on the System Properties > Hardware tab.

You can see that the camera driver has been located when the Soft- Hard Technology MV4.0 entry is shown in the Other devices branch. 55

2) Select the SoftHard Technology MV4.0 entry in the Device Manager, and click the Update Driver button in the Device Manager. " The Found New Hardware Wizard dialog box appears. 3) The question Can Windows connect to Windows Update to search for software? should be answered with No, not this time; after that click the Next> button. Help with Problems

You then answer the question Can Windows connect to Windows Update to search for software?, with No, not this time.

" A new dialog box entitled Found New Hardware Wizard will open. 4) Select the Install from a list or specific location (Advanced) option, and click the Next > button. In this dialog box, select the Install from a list or specific location (Advanced) option. 56 Help with Problems

" A new dialog box entitled Found New Hardware Wizard will open. 5) Select the Don’t search. I will choose the driver to install option. In this dialog box, select the Don’t search. I will choose the driver to install option.

6) Click the Next > button. The OLYMPUS COR- PORATION FireWire 2.0.26 (SoftHard) entry is offered in the Model list located in this dialog box. Select it. 57

7) Select the OLYMPUS CORPORATION FireWire Camera 2.0.26 (SoftHard) entry in the Model list. Help with Problems

8) Click the Next > button. " The next Found New Hardware Wizard dialog box will be opened in the background, displaying the message to wait while the Wizard installs the software. The Hardware Installation dialog box shown here may pop up.

9) As the information given in this dialog box is not important for the installation, simply click the Continue Anyway button. " You will return to the Found New Hardware Wizard dialog box. 58

10) Click the Next > button. Help with Problems

" After completing the installation of the driver for the DP25 camera, the following dialog box is opened.

11) Click the Finish button to end the Hardware Update Wizard assistant. 12) Switch to the Device Manager. " In the tree view, you will find the OLYMPUS CORPORATION FireWire Camera 2.0.26 (SoftHard) entry under the Imaging Devices branch. 13) Restart the PC. " The camera is now ready for use. 59 Help with Problems

After successfully installing the driver for the DP25, the OLYMPUS CORPORA- TION FireWire Camera 2.0.26 (SoftHard) entry is displayed in the Device Manager dialog box. The camera is now ready for use. 60 Declarations of Conformity Declarations of Conformity

WEEE declaration Waste Electrical In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic and Electronic Equipment, this symbol indicates that the product must not Equipment be disposed of as unsorted municipal waste, but should be collected separately. Refer to your local distributor for return.

FCC compliance This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense. PLEASE NOTE Changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.

CE conformity This device complies with the requirements of directive 89/336/EEC concerning electromagnetic compatibility. The CE marking indicates compliances with the above directive. 61 OLYMPUS CORPORATION Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku- Tokyo, Japan OLYMPUS LIFE AND MATERIAL SCIENCE EUROPA GMBH Postfach 10 49 08, 20034, Hamburg, Germany OLYMPUS AMERICA INC. 3500 Corporate Parkway, Center Valley, PA18034-0610, U.S.A. OLYMPUS SINGAPORE PTE LTD. 491 B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 OLYMPUS AUSTRALIA PTY. LTD. 31 Gilby Road, Mt. Waverley, VIC 3149, Melbourne, Australia OLYMPUS LATIN AMERICA, INC. 5301 Blue Lagoon Drive, Suite 290, Miami, FL 33126, U.S.A. OLYMPUS (BEIJING) SALES & SERVICE CO., LTD. 12-13F, NCI Tower, A12 Jianguomenwai Avenue, Chaoyang District, Beijing, 100022, China

Printed in Germany DP25_E_0706